AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053583

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053583.t1 MTR_5g007880 96.154 312 12 0 1 312 1 312 0.0 627
MS.gene053583.t1 MTR_5g007880 96.071 280 11 0 1 280 1 280 0.0 562
MS.gene053583.t1 MTR_5g014220 76.923 39 9 0 115 153 43 81 2.71e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053583.t1 AT2G43190 54.274 234 102 2 75 308 62 290 8.15e-84 254
MS.gene053583.t1 AT2G43190 54.274 234 102 2 75 308 62 290 8.15e-84 254
MS.gene053583.t1 AT2G43190 57.407 216 91 1 93 308 75 289 2.27e-83 253
MS.gene053583.t1 AT2G43190 57.407 216 91 1 93 308 75 289 2.27e-83 253
MS.gene053583.t1 AT2G43190 44.017 234 91 2 75 308 62 255 8.57e-57 184

Find 58 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTGAAACTGCAGAAGCTTT+TGG 0.202603 4.4:+21417505 MS.gene053583:CDS
GAAAGAGGAGGTTCAGTTTC+CGG 0.213770 4.4:+21415960 MS.gene053583:CDS
TGCCACCAGTTCCAGTAAAA+TGG 0.225932 4.4:-21417474 None:intergenic
AACTTACTTATGGAACTCTT+TGG 0.264485 4.4:-21416201 None:intergenic
GAAAATCTGCTCACAACTAA+TGG 0.271857 4.4:+21415778 MS.gene053583:CDS
AAAATCTGCTCACAACTAAT+GGG 0.354040 4.4:+21415779 MS.gene053583:CDS
TTGTACTACAAGTTGATTGC+TGG 0.357928 4.4:+21418525 MS.gene053583:CDS
ACAGAAAGTTGGGCATATTC+TGG 0.385740 4.4:-21415745 None:intergenic
TCCGATGATGGACGACATAA+AGG 0.385790 4.4:-21414932 None:intergenic
GAAACGTGCAATGGAGGCTT+TGG 0.387822 4.4:+21414751 MS.gene053583:CDS
CATACTGAATTACCTTTGTT+GGG 0.393983 4.4:-21414959 None:intergenic
AAGAAGTTCATGCAATATTC+CGG 0.396909 4.4:-21415979 None:intergenic
GAAGTAGAGGGTGGTGGAGT+AGG 0.406154 4.4:-21414893 None:intergenic
CATTTACAGTCACAGAAAGT+TGG 0.409130 4.4:-21415756 None:intergenic
TCATACTGAATTACCTTTGT+TGG 0.421242 4.4:-21414960 None:intergenic
AAACTAGATTCAAGAAAGGT+TGG 0.424013 4.4:+21418569 MS.gene053583:CDS
ATTTACAGTCACAGAAAGTT+GGG 0.425288 4.4:-21415755 None:intergenic
ACTTACTTATGGAACTCTTT+GGG 0.426320 4.4:-21416200 None:intergenic
TTCATTCAAAACGTTCTAAG+AGG 0.430799 4.4:+21416132 MS.gene053583:CDS
CAGTACAGTACCTTGTGAAA+TGG 0.436121 4.4:-21415578 None:intergenic
TGCTGGAAAGCCACTCTGCT+TGG 0.452437 4.4:+21418542 MS.gene053583:CDS
TTGTTCCAGGAATAATCAAT+TGG 0.458394 4.4:+21416641 MS.gene053583:intron
AAGTTGTTGCTTAAATCCAC+TGG 0.460350 4.4:+21416507 MS.gene053583:CDS
GCCTTTATGTCGTCCATCAT+CGG 0.460662 4.4:+21414931 MS.gene053583:CDS
GCCACCAGTTCCAGTAAAAT+GGG 0.464672 4.4:-21417473 None:intergenic
TTCTTCATCTTCTCAGACAA+GGG 0.467025 4.4:+21416100 MS.gene053583:CDS
AATTGCAGTTGTTCCGAAGA+AGG 0.469187 4.4:+21418493 MS.gene053583:intron
TATCAATGGCTGCTGAATCA+AGG 0.478212 4.4:+21414729 None:intergenic
ACAAATACAGAACCCTTCTT+CGG 0.485262 4.4:-21418506 None:intergenic
CAATTGGCTCAATGTCTCCT+AGG 0.493446 4.4:+21416657 MS.gene053583:CDS
TTTCTTCATCTTCTCAGACA+AGG 0.512698 4.4:+21416099 MS.gene053583:CDS
TCTTTCCACATGTCATGCAT+TGG 0.514242 4.4:-21416477 None:intergenic
TTGTTGGGTGTATCCGATGA+TGG 0.532259 4.4:-21414944 None:intergenic
ATTGCAGTTGTTCCGAAGAA+GGG 0.540540 4.4:+21418494 MS.gene053583:intron
CTTGCACAAAATTATCAAGA+AGG 0.550602 4.4:-21416067 None:intergenic
ATTGCATGAACTTCTTCAGA+AGG 0.551548 4.4:+21415986 MS.gene053583:CDS
TCATTAGAAGTAGAGGGTGG+TGG 0.559781 4.4:-21414899 None:intergenic
AGATAAACTAGATTCAAGAA+AGG 0.561327 4.4:+21418565 MS.gene053583:CDS
ACTGAAGCTAATAAATTCCA+AGG 0.566213 4.4:+21417535 MS.gene053583:CDS
TTTGGTCGTGCCATTTCACA+AGG 0.566627 4.4:+21415568 MS.gene053583:CDS
CTCCTAGGTGCAGATCTACA+TGG 0.569680 4.4:+21416672 MS.gene053583:CDS
GGACGGATCATTAGAAGTAG+AGG 0.570525 4.4:-21414906 None:intergenic
ATCAAGGAAACGTGCAATGG+AGG 0.577785 4.4:+21414745 MS.gene053583:CDS
TAATCTGTAGAGAAAGAAGA+TGG 0.577837 4.4:+21415721 MS.gene053583:intron
TTGCATGAACTTCTTCAGAA+GGG 0.595042 4.4:+21415987 MS.gene053583:CDS
AGTTGTTGCTTAAATCCACT+GGG 0.610453 4.4:+21416508 MS.gene053583:CDS
TCAAGCCAATGCATGACATG+TGG 0.623887 4.4:+21416472 MS.gene053583:CDS
GGACGACATAAAGGCAAGGA+CGG 0.625054 4.4:-21414923 None:intergenic
GACGGATCATTAGAAGTAGA+GGG 0.632740 4.4:-21414905 None:intergenic
GCAGCACAAAAGTACATGCA+AGG 0.640542 4.4:+21416014 MS.gene053583:CDS
TCTGCTCACAACTAATGGGG+AGG 0.647144 4.4:+21415783 MS.gene053583:CDS
TGAATCAAGGAAACGTGCAA+TGG 0.657298 4.4:+21414742 MS.gene053583:CDS
AAATCTGCTCACAACTAATG+GGG 0.672764 4.4:+21415780 MS.gene053583:CDS
TCATCGGATACACCCAACAA+AGG 0.673500 4.4:+21414947 MS.gene053583:CDS
TAGTTTATCTCCAAGCAGAG+TGG 0.678445 4.4:-21418552 None:intergenic
TGATGGACGACATAAAGGCA+AGG 0.683011 4.4:-21414927 None:intergenic
CACCATGTAGATCTGCACCT+AGG 0.706017 4.4:-21416674 None:intergenic
GGATCATTAGAAGTAGAGGG+TGG 0.734876 4.4:-21414902 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTAAAAAATCAAAGTATT+TGG - chr4.4:21418477-21418496 None:intergenic 10.0%
!!! TTGATAATTAATGAATTATA+AGG + chr4.4:21417698-21417717 MS.gene053583:intron 10.0%
!! AAAAGAAAAGAAAAACAAAA+GGG + chr4.4:21414802-21414821 MS.gene053583:CDS 15.0%
!! GAGTAAAAAAAAAAATCAAT+AGG - chr4.4:21418141-21418160 None:intergenic 15.0%
!! TAAAAGAAAAGAAAAACAAA+AGG + chr4.4:21414801-21414820 MS.gene053583:CDS 15.0%
!!! AAGAATTTTGAAAAGAAAAT+AGG - chr4.4:21418245-21418264 None:intergenic 15.0%
!!! AATATTTGTATTTGTTTTGA+AGG - chr4.4:21417366-21417385 None:intergenic 15.0%
!!! TTTGCTTATGATTATTTTTA+AGG + chr4.4:21415261-21415280 MS.gene053583:intron 15.0%
!!! TTTTTTATTTCCTTTTCTTA+GGG + chr4.4:21415533-21415552 MS.gene053583:intron 15.0%
!! AATATTTCATATGCATAGAA+AGG + chr4.4:21416880-21416899 MS.gene053583:intron 20.0%
!! AGTCATTCTTATAAACAATA+TGG + chr4.4:21417985-21418004 MS.gene053583:intron 20.0%
!! ATAATTCCAAAAAGTCAATA+TGG + chr4.4:21415833-21415852 MS.gene053583:intron 20.0%
!! ATGAAACAACATAAAAAACA+TGG + chr4.4:21416164-21416183 MS.gene053583:CDS 20.0%
!! ATTTGTAACTTCAATTTATG+TGG + chr4.4:21415677-21415696 MS.gene053583:intron 20.0%
!! TTTGTAACTTCAATTTATGT+GGG + chr4.4:21415678-21415697 MS.gene053583:intron 20.0%
!!! AAAGCTCTAGATAAATAAAA+TGG - chr4.4:21416781-21416800 None:intergenic 20.0%
!!! ATGTTTTTTATGTTGTTTCA+TGG - chr4.4:21416165-21416184 None:intergenic 20.0%
!!! ATTCTATTTTAAGTAGTGAA+AGG - chr4.4:21417565-21417584 None:intergenic 20.0%
!!! ATTTGCTACATATAATTGTA+CGG + chr4.4:21418081-21418100 MS.gene053583:intron 20.0%
!!! CTCTATTTTTGTTTTGTTTT+TGG + chr4.4:21416720-21416739 MS.gene053583:intron 20.0%
!!! CTTTTTTATTTCCTTTTCTT+AGG + chr4.4:21415532-21415551 MS.gene053583:intron 20.0%
!!! TACATTTTATGGAAGTATTT+TGG + chr4.4:21416239-21416258 MS.gene053583:intron 20.0%
!!! TCATAATTTTCTTTTCTTTC+AGG - chr4.4:21414847-21414866 None:intergenic 20.0%
!!! TTTATTTATCTAGAGCTTTT+TGG + chr4.4:21416782-21416801 MS.gene053583:intron 20.0%
!!! TTTCATTTTCTTAGTAAAAG+AGG - chr4.4:21418268-21418287 None:intergenic 20.0%
!!! TTTTGATCTAACTAAAACTT+TGG - chr4.4:21418221-21418240 None:intergenic 20.0%
! AAAAAAAAATCCCAAGTGTA+AGG - chr4.4:21417155-21417174 None:intergenic 25.0%
! AAAACTTCAACTTAAGCAAA+TGG - chr4.4:21418397-21418416 None:intergenic 25.0%
! AAGTCAAATTAGAACAAACA+GGG - chr4.4:21415914-21415933 None:intergenic 25.0%
! ACAATTATATGTAGCAAATC+TGG - chr4.4:21418080-21418099 None:intergenic 25.0%
! ACATAAAAAACATGGATCAA+TGG + chr4.4:21416172-21416191 MS.gene053583:CDS 25.0%
! AGATAAACTAGATTCAAGAA+AGG + chr4.4:21418565-21418584 MS.gene053583:CDS 25.0%
! ATTTAAATGAGAAACATTCC+CGG - chr4.4:21417087-21417106 None:intergenic 25.0%
! GCTTATATGTTTGAATGTAA+AGG + chr4.4:21417862-21417881 MS.gene053583:intron 25.0%
! TAACTAGCAGACAATTATTT+AGG - chr4.4:21417402-21417421 None:intergenic 25.0%
! TAAGTCAAATTAGAACAAAC+AGG - chr4.4:21415915-21415934 None:intergenic 25.0%
!! ACTATGCTGAATTTTCAAAA+CGG - chr4.4:21417735-21417754 None:intergenic 25.0%
!! CAACTCTTTTCTAAATTCAA+AGG - chr4.4:21417330-21417349 None:intergenic 25.0%
!! GAACTAATGTTGAATGTTTT+AGG + chr4.4:21417186-21417205 MS.gene053583:intron 25.0%
!! GTAAAAAAAGTACATGTATG+TGG + chr4.4:21415221-21415240 MS.gene053583:intron 25.0%
!! GTCTCTAAACTTACATTTTA+TGG + chr4.4:21416228-21416247 MS.gene053583:intron 25.0%
!! TACTTATATTTAAAGCCTGA+TGG - chr4.4:21415204-21415223 None:intergenic 25.0%
!! TTTTCATTTCGTCTTTCATT+GGG + chr4.4:21416309-21416328 MS.gene053583:intron 25.0%
!! TTTTTCATTTCGTCTTTCAT+TGG + chr4.4:21416308-21416327 MS.gene053583:intron 25.0%
!!! CTTATGATTATTTTTAAGGC+TGG + chr4.4:21415265-21415284 MS.gene053583:intron 25.0%
!!! GAGTTCTCACTTTTTATTTA+GGG + chr4.4:21418186-21418205 MS.gene053583:intron 25.0%
!!! TGAGTTCTCACTTTTTATTT+AGG + chr4.4:21418185-21418204 MS.gene053583:intron 25.0%
!!! TTCTACATTTTGATTTCTGA+AGG + chr4.4:21415637-21415656 MS.gene053583:intron 25.0%
AAAATCTGCTCACAACTAAT+GGG + chr4.4:21415779-21415798 MS.gene053583:CDS 30.0%
AAACTAGATTCAAGAAAGGT+TGG + chr4.4:21418569-21418588 MS.gene053583:CDS 30.0%
AACTTACTTATGGAACTCTT+TGG - chr4.4:21416204-21416223 None:intergenic 30.0%
AAGAAGTTCATGCAATATTC+CGG - chr4.4:21415982-21416001 None:intergenic 30.0%
ACTGAAGCTAATAAATTCCA+AGG + chr4.4:21417535-21417554 MS.gene053583:CDS 30.0%
ACTTACTTATGGAACTCTTT+GGG - chr4.4:21416203-21416222 None:intergenic 30.0%
ATATGGCTGAGATTCTTATT+TGG + chr4.4:21418002-21418021 MS.gene053583:intron 30.0%
ATGTGACACTTGCATTATTA+TGG + chr4.4:21418324-21418343 MS.gene053583:intron 30.0%
ATTTACAGTCACAGAAAGTT+GGG - chr4.4:21415758-21415777 None:intergenic 30.0%
CATACTGAATTACCTTTGTT+GGG - chr4.4:21414962-21414981 None:intergenic 30.0%
CATTAATACTTGAGAAACAC+AGG - chr4.4:21417616-21417635 None:intergenic 30.0%
CTTGCACAAAATTATCAAGA+AGG - chr4.4:21416070-21416089 None:intergenic 30.0%
GATATTCTTAGATTCATGCT+AGG + chr4.4:21416331-21416350 MS.gene053583:intron 30.0%
TAAATACTTGCATGACTGTT+TGG - chr4.4:21415436-21415455 None:intergenic 30.0%
TAATCTGTAGAGAAAGAAGA+TGG + chr4.4:21415721-21415740 MS.gene053583:intron 30.0%
TACTAAAGATATAACCTGAC+AGG - chr4.4:21418042-21418061 None:intergenic 30.0%
TCATACTGAATTACCTTTGT+TGG - chr4.4:21414963-21414982 None:intergenic 30.0%
TTATATGTAGCAAATCTGGT+TGG - chr4.4:21418076-21418095 None:intergenic 30.0%
TTATTCCTGGAACAATAAAC+AGG - chr4.4:21416636-21416655 None:intergenic 30.0%
TTGCATTATTATGGCTGAAT+TGG + chr4.4:21418333-21418352 MS.gene053583:intron 30.0%
TTGGATGTTAATTATTCCCA+CGG + chr4.4:21416836-21416855 MS.gene053583:intron 30.0%
TTGTTCCAGGAATAATCAAT+TGG + chr4.4:21416641-21416660 MS.gene053583:intron 30.0%
! GAACTATTTTACGCAATCAT+TGG + chr4.4:21416981-21417000 MS.gene053583:intron 30.0%
! GAGAAGATGTATTTTATGAC+AGG + chr4.4:21417908-21417927 MS.gene053583:intron 30.0%
! GAGCTTTTTGGATAATTACA+AGG + chr4.4:21416794-21416813 MS.gene053583:intron 30.0%
! TAAACCCAAATGCATTTTTC+AGG + chr4.4:21415466-21415485 MS.gene053583:intron 30.0%
! TGTAAACTAACCCATTTTAC+TGG + chr4.4:21417463-21417482 MS.gene053583:CDS 30.0%
! TGTATGCTTTTCTTATACCA+GGG + chr4.4:21417661-21417680 MS.gene053583:intron 30.0%
! TTCATTCAAAACGTTCTAAG+AGG + chr4.4:21416132-21416151 MS.gene053583:CDS 30.0%
! TTCCTTTTCTTAGGGAATTT+CGG + chr4.4:21415541-21415560 MS.gene053583:intron 30.0%
! TTGACTCGAAAGTCATTAAT+AGG + chr4.4:21418296-21418315 MS.gene053583:intron 30.0%
! TTTCTAAGAACACCAAAAGT+AGG - chr4.4:21416950-21416969 None:intergenic 30.0%
!! AAGGACAGTTATTTTGTCAT+TGG + chr4.4:21416813-21416832 MS.gene053583:intron 30.0%
!! ACAGTTATTTTGTCATTGGT+TGG + chr4.4:21416817-21416836 MS.gene053583:intron 30.0%
!! ACTCAGCTGTTTCTAATTTA+CGG + chr4.4:21418157-21418176 MS.gene053583:intron 30.0%
!! CTTCTTGATAATTTTGTGCA+AGG + chr4.4:21416068-21416087 MS.gene053583:CDS 30.0%
!! TTTTGATGACTTATCAGTTC+TGG + chr4.4:21418413-21418432 MS.gene053583:intron 30.0%
!!! ATGATTATTTTTAAGGCTGG+AGG + chr4.4:21415268-21415287 MS.gene053583:intron 30.0%
!!! CTACATGGTGCTATTATTTT+AGG + chr4.4:21416687-21416706 MS.gene053583:CDS 30.0%
!!! GATTGACCATATTGACTTTT+TGG - chr4.4:21415842-21415861 None:intergenic 30.0%
!!! GTGTTCAGTTGCTTTTAAAA+TGG + chr4.4:21417815-21417834 MS.gene053583:intron 30.0%
!!! TGATTATTTTTAAGGCTGGA+GGG + chr4.4:21415269-21415288 MS.gene053583:intron 30.0%
!!! TGCTTTTAAAATGGAACTTG+TGG + chr4.4:21417824-21417843 MS.gene053583:intron 30.0%
!!! TTGGCTTATTTTTTAGCTCA+TGG + chr4.4:21415002-21415021 MS.gene053583:intron 30.0%
!!! TTTTAGAGTTCAGACTTCAA+AGG - chr4.4:21415866-21415885 None:intergenic 30.0%
AAATCTGCTCACAACTAATG+GGG + chr4.4:21415780-21415799 MS.gene053583:CDS 35.0%
AAGTAGTGAAAGGATTACCT+TGG - chr4.4:21417555-21417574 None:intergenic 35.0%
ACAAACCTGTTTATTGTTCC+AGG + chr4.4:21416628-21416647 MS.gene053583:intron 35.0%
ACAAATACAGAACCCTTCTT+CGG - chr4.4:21418509-21418528 None:intergenic 35.0%
AGAGACCTTGAACTTACTTA+TGG - chr4.4:21416214-21416233 None:intergenic 35.0%
AGGTAATTCAGTATGACACT+TGG + chr4.4:21414967-21414986 MS.gene053583:intron 35.0%
ATAAAGACATAAGCATCCGT+GGG - chr4.4:21416855-21416874 None:intergenic 35.0%
ATTGCATGAACTTCTTCAGA+AGG + chr4.4:21415986-21416005 MS.gene053583:CDS 35.0%
CAAAACTAAGCCAATCCAAA+CGG + chr4.4:21415169-21415188 MS.gene053583:intron 35.0%
CATTTACAGTCACAGAAAGT+TGG - chr4.4:21415759-21415778 None:intergenic 35.0%
CTAGCAGACAATTATTTAGG+AGG - chr4.4:21417399-21417418 None:intergenic 35.0%
CTAGCCTGAAAAATGCATTT+GGG - chr4.4:21415473-21415492 None:intergenic 35.0%
GAAAATCTGCTCACAACTAA+TGG + chr4.4:21415778-21415797 MS.gene053583:CDS 35.0%
GACAATTATTTAGGAGGTAG+AGG - chr4.4:21417393-21417412 None:intergenic 35.0%
GAGTTCCATAAGTAAGTTCA+AGG + chr4.4:21416206-21416225 MS.gene053583:intron 35.0%
GGGATATAGTAGTAATGACA+AGG - chr4.4:21416266-21416285 None:intergenic 35.0%
GGTAATTCAGTATGACACTT+GGG + chr4.4:21414968-21414987 MS.gene053583:intron 35.0%
TAAAATGGAACTTGTGGACA+TGG + chr4.4:21417830-21417849 MS.gene053583:intron 35.0%
TAGTAAGATGAGCTTCTGAT+TGG + chr4.4:21417035-21417054 MS.gene053583:intron 35.0%
TATACAATAACAGTACCCAG+TGG - chr4.4:21416526-21416545 None:intergenic 35.0%
TCTAGCCTGAAAAATGCATT+TGG - chr4.4:21415474-21415493 None:intergenic 35.0%
TTCTTCATCTTCTCAGACAA+GGG + chr4.4:21416100-21416119 MS.gene053583:CDS 35.0%
TTGAGCCAATTGATTATTCC+TGG - chr4.4:21416649-21416668 None:intergenic 35.0%
TTGCATGAACTTCTTCAGAA+GGG + chr4.4:21415987-21416006 MS.gene053583:CDS 35.0%
TTTCTTCATCTTCTCAGACA+AGG + chr4.4:21416099-21416118 MS.gene053583:CDS 35.0%
! AAGTTGTTGCTTAAATCCAC+TGG + chr4.4:21416507-21416526 MS.gene053583:CDS 35.0%
! AGTGTAAGGAGCATGAAAAA+AGG - chr4.4:21417141-21417160 None:intergenic 35.0%
! AGTTGTTGCTTAAATCCACT+GGG + chr4.4:21416508-21416527 MS.gene053583:CDS 35.0%
! ATGCTGAATTTTCAAAACGG+AGG - chr4.4:21417732-21417751 None:intergenic 35.0%
! GATGTATTTTATGACAGGCA+TGG + chr4.4:21417913-21417932 MS.gene053583:intron 35.0%
! GGTTTAGCATTTTCTACTGT+AGG - chr4.4:21416760-21416779 None:intergenic 35.0%
! GTGTATGCTTTTCTTATACC+AGG + chr4.4:21417660-21417679 MS.gene053583:intron 35.0%
! TTAGGGAATTTCGGTCTTTT+TGG + chr4.4:21415550-21415569 MS.gene053583:intron 35.0%
! TTTAAAGCCTGATGGAACAA+TGG - chr4.4:21415196-21415215 None:intergenic 35.0%
! TTTTCATGCTCCTTACACTT+GGG + chr4.4:21417142-21417161 MS.gene053583:intron 35.0%
! TTTTTCATGCTCCTTACACT+TGG + chr4.4:21417141-21417160 MS.gene053583:intron 35.0%
!! AGCTGTTTCTACATTTCAAG+AGG + chr4.4:21415322-21415341 MS.gene053583:intron 35.0%
!! GCTGTTTCTACATTTCAAGA+GGG + chr4.4:21415323-21415342 MS.gene053583:intron 35.0%
!! TTGTACTACAAGTTGATTGC+TGG + chr4.4:21418525-21418544 MS.gene053583:CDS 35.0%
AAAAAGGTTATGCATGCCAC+TGG - chr4.4:21417125-21417144 None:intergenic 40.0%
AAAAGGTTATGCATGCCACT+GGG - chr4.4:21417124-21417143 None:intergenic 40.0%
AAACACAGGAATAACAGCTC+TGG - chr4.4:21417602-21417621 None:intergenic 40.0%
AATTGCAGTTGTTCCGAAGA+AGG + chr4.4:21418493-21418512 MS.gene053583:intron 40.0%
ACACAGAATTGTGATGCTCA+AGG - chr4.4:21418373-21418392 None:intergenic 40.0%
ACAGAAAGTTGGGCATATTC+TGG - chr4.4:21415748-21415767 None:intergenic 40.0%
ATTGCAGTTGTTCCGAAGAA+GGG + chr4.4:21418494-21418513 MS.gene053583:intron 40.0%
CAAATTTGTACAGCAAACCC+TGG - chr4.4:21417681-21417700 None:intergenic 40.0%
CACTATTAGATTGTCCTGTC+AGG + chr4.4:21418025-21418044 MS.gene053583:intron 40.0%
CAGTACAGTACCTTGTGAAA+TGG - chr4.4:21415581-21415600 None:intergenic 40.0%
CATAAAGACATAAGCATCCG+TGG - chr4.4:21416856-21416875 None:intergenic 40.0%
GACCGAAATTCCCTAAGAAA+AGG - chr4.4:21415546-21415565 None:intergenic 40.0%
GACGGATCATTAGAAGTAGA+GGG - chr4.4:21414908-21414927 None:intergenic 40.0%
GATGACCTAATCAGCAATCA+CGG + chr4.4:21416422-21416441 MS.gene053583:intron 40.0%
TACTATATCCCTGCAGTTTG+TGG + chr4.4:21416275-21416294 MS.gene053583:intron 40.0%
TAGTTTATCTCCAAGCAGAG+TGG - chr4.4:21418555-21418574 None:intergenic 40.0%
TCAAGAGGGAATAAGCAATG+TGG + chr4.4:21415337-21415356 MS.gene053583:intron 40.0%
TCTTTCCACATGTCATGCAT+TGG - chr4.4:21416480-21416499 None:intergenic 40.0%
TGAATCAAGGAAACGTGCAA+TGG + chr4.4:21414742-21414761 MS.gene053583:CDS 40.0%
TGAGTCATCTAGACAGAGTT+TGG - chr4.4:21417296-21417315 None:intergenic 40.0%
TTCACAGGTTTGAGTACATG+CGG - chr4.4:21416925-21416944 None:intergenic 40.0%
! AACCTGTGAAAGCCTACTTT+TGG + chr4.4:21416935-21416954 MS.gene053583:intron 40.0%
! ATGAACCGTGATTGCTGATT+AGG - chr4.4:21416430-21416449 None:intergenic 40.0%
! CTAACCCATTTTACTGGAAC+TGG + chr4.4:21417469-21417488 MS.gene053583:CDS 40.0%
! CTGCTTGTTACTCAAGAAAG+CGG - chr4.4:21417958-21417977 None:intergenic 40.0%
! GTGAAACTGCAGAAGCTTTT+GGG + chr4.4:21417506-21417525 MS.gene053583:CDS 40.0%
!! CAGTTTTCTTCTGAAAGAGG+AGG + chr4.4:21415948-21415967 MS.gene053583:intron 40.0%
!! CTGCAGTTTTCTTCTGAAAG+AGG + chr4.4:21415945-21415964 MS.gene053583:intron 40.0%
!!! CACTTGGGATCTGTTTTGAT+TGG + chr4.4:21414983-21415002 MS.gene053583:intron 40.0%
AAACGGACCATTGTTCCATC+AGG + chr4.4:21415186-21415205 MS.gene053583:intron 45.0%
ACACTCCACAACTGCATAAC+AGG - chr4.4:21417446-21417465 None:intergenic 45.0%
AGAGGGAATAAGCAATGTGG+AGG + chr4.4:21415340-21415359 MS.gene053583:intron 45.0%
ATACCTAGGTCTTCTTCTCC+GGG + chr4.4:21417066-21417085 MS.gene053583:intron 45.0%
ATCAAGGAAACGTGCAATGG+AGG + chr4.4:21414745-21414764 MS.gene053583:CDS 45.0%
ATCCACCTGTTATGCAGTTG+TGG + chr4.4:21417438-21417457 MS.gene053583:intron 45.0%
CACCAAAAGTAGGCTTTCAC+AGG - chr4.4:21416940-21416959 None:intergenic 45.0%
CATACCTAGGTCTTCTTCTC+CGG + chr4.4:21417065-21417084 MS.gene053583:intron 45.0%
GAAAGAGGAGGTTCAGTTTC+CGG + chr4.4:21415960-21415979 MS.gene053583:CDS 45.0%
GAACAATGGTCCGTTTGGAT+TGG - chr4.4:21415182-21415201 None:intergenic 45.0%
GCCACCAGTTCCAGTAAAAT+GGG - chr4.4:21417476-21417495 None:intergenic 45.0%
GCCTTTATGTCGTCCATCAT+CGG + chr4.4:21414931-21414950 MS.gene053583:CDS 45.0%
GGACGGATCATTAGAAGTAG+AGG - chr4.4:21414909-21414928 None:intergenic 45.0%
GGATCATTAGAAGTAGAGGG+TGG - chr4.4:21414905-21414924 None:intergenic 45.0%
TCAAGCCAATGCATGACATG+TGG + chr4.4:21416472-21416491 MS.gene053583:CDS 45.0%
TCATCGGATACACCCAACAA+AGG + chr4.4:21414947-21414966 MS.gene053583:CDS 45.0%
TCATTAGAAGTAGAGGGTGG+TGG - chr4.4:21414902-21414921 None:intergenic 45.0%
TGATGGAACAATGGTCCGTT+TGG - chr4.4:21415187-21415206 None:intergenic 45.0%
TGCCACCAGTTCCAGTAAAA+TGG - chr4.4:21417477-21417496 None:intergenic 45.0%
TTGTTGGGTGTATCCGATGA+TGG - chr4.4:21414947-21414966 None:intergenic 45.0%
! ACCCATTTTACTGGAACTGG+TGG + chr4.4:21417472-21417491 MS.gene053583:CDS 45.0%
! CAATTGGCTCAATGTCTCCT+AGG + chr4.4:21416657-21416676 MS.gene053583:CDS 45.0%
! CGTGAAACTGCAGAAGCTTT+TGG + chr4.4:21417505-21417524 MS.gene053583:CDS 45.0%
! GATGAGCTTCTGATTGGTTG+AGG + chr4.4:21417041-21417060 MS.gene053583:intron 45.0%
! GATTGGTTGAGGTCATACCT+AGG + chr4.4:21417052-21417071 MS.gene053583:intron 45.0%
! GCAGCACAAAAGTACATGCA+AGG + chr4.4:21416014-21416033 MS.gene053583:CDS 45.0%
! TCCTTCCTTTTCATTCCCAG+TGG + chr4.4:21417106-21417125 MS.gene053583:intron 45.0%
! TGATGGACGACATAAAGGCA+AGG - chr4.4:21414930-21414949 None:intergenic 45.0%
!! TCCGATGATGGACGACATAA+AGG - chr4.4:21414935-21414954 None:intergenic 45.0%
!! TTTGGTCGTGCCATTTCACA+AGG + chr4.4:21415568-21415587 MS.gene053583:CDS 45.0%
ATTCCCGGAGAAGAAGACCT+AGG - chr4.4:21417072-21417091 None:intergenic 50.0%
CACCATGTAGATCTGCACCT+AGG - chr4.4:21416677-21416696 None:intergenic 50.0%
CTCCACAACTGCATAACAGG+TGG - chr4.4:21417443-21417462 None:intergenic 50.0%
CTCCTAGGTGCAGATCTACA+TGG + chr4.4:21416672-21416691 MS.gene053583:CDS 50.0%
GAAACGTGCAATGGAGGCTT+TGG + chr4.4:21414751-21414770 MS.gene053583:CDS 50.0%
GCATGCCACTGGGAATGAAA+AGG - chr4.4:21417114-21417133 None:intergenic 50.0%
GCCACTGGGAATGAAAAGGA+AGG - chr4.4:21417110-21417129 None:intergenic 50.0%
GGACGACATAAAGGCAAGGA+CGG - chr4.4:21414926-21414945 None:intergenic 50.0%
TCTCACTGCCACAAACTGCA+GGG - chr4.4:21416286-21416305 None:intergenic 50.0%
TCTGCTCACAACTAATGGGG+AGG + chr4.4:21415783-21415802 MS.gene053583:CDS 50.0%
TTCTCACTGCCACAAACTGC+AGG - chr4.4:21416287-21416306 None:intergenic 50.0%
TGCTGGAAAGCCACTCTGCT+TGG + chr4.4:21418542-21418561 MS.gene053583:CDS 55.0%
!! GAAGTAGAGGGTGGTGGAGT+AGG - chr4.4:21414896-21414915 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 21414734 21418598 21414734 ID=MS.gene053583
chr4.4 mRNA 21414734 21418598 21414734 ID=MS.gene053583.t1;Parent=MS.gene053583
chr4.4 exon 21414734 21414968 21414734 ID=MS.gene053583.t1.exon1;Parent=MS.gene053583.t1
chr4.4 CDS 21414734 21414968 21414734 ID=cds.MS.gene053583.t1;Parent=MS.gene053583.t1
chr4.4 exon 21415554 21415589 21415554 ID=MS.gene053583.t1.exon2;Parent=MS.gene053583.t1
chr4.4 CDS 21415554 21415589 21415554 ID=cds.MS.gene053583.t1;Parent=MS.gene053583.t1
chr4.4 exon 21415731 21415804 21415731 ID=MS.gene053583.t1.exon3;Parent=MS.gene053583.t1
chr4.4 CDS 21415731 21415804 21415731 ID=cds.MS.gene053583.t1;Parent=MS.gene053583.t1
chr4.4 exon 21415951 21416216 21415951 ID=MS.gene053583.t1.exon4;Parent=MS.gene053583.t1
chr4.4 CDS 21415951 21416216 21415951 ID=cds.MS.gene053583.t1;Parent=MS.gene053583.t1
chr4.4 exon 21416461 21416529 21416461 ID=MS.gene053583.t1.exon5;Parent=MS.gene053583.t1
chr4.4 CDS 21416461 21416529 21416461 ID=cds.MS.gene053583.t1;Parent=MS.gene053583.t1
chr4.4 exon 21416650 21416708 21416650 ID=MS.gene053583.t1.exon6;Parent=MS.gene053583.t1
chr4.4 CDS 21416650 21416708 21416650 ID=cds.MS.gene053583.t1;Parent=MS.gene053583.t1
chr4.4 exon 21417455 21417556 21417455 ID=MS.gene053583.t1.exon7;Parent=MS.gene053583.t1
chr4.4 CDS 21417455 21417556 21417455 ID=cds.MS.gene053583.t1;Parent=MS.gene053583.t1
chr4.4 exon 21418501 21418598 21418501 ID=MS.gene053583.t1.exon8;Parent=MS.gene053583.t1
chr4.4 CDS 21418501 21418598 21418501 ID=cds.MS.gene053583.t1;Parent=MS.gene053583.t1
Gene Sequence

>MS.gene053583

ATGGCTGCTGAATCAAGGAAACGTGCAATGGAGGCTTTGGAGAGAAGAATTCAAACTGAACATAAACTAAAAGAAAAGAAAAACAAAAGGGATATAAATCATCAACATATCCTGAAAGAAAAGAAAATTATGAAGTCTATAAATGAAGATGTAAAATCACCTACTCCACCACCCTCTACTTCTAATGATCCGTCCTTGCCTTTATGTCGTCCATCATCGGATACACCCAACAAAGGTAATTCAGTATGACACTTGGGATCTGTTTTGATTGGCTTATTTTTTAGCTCATGGAAATAAATACGCTTTTATGTTAATTCTTATGTTCTCACTAACAAAAATTGTATCTTCATAAGCTGTTTTTTCATAAACTACATTGACAAGCTTATGAAAATACATAAAAGTTTATTTATTTGCTTAAGCTGTTCTGCATAAGCTCAAAACTAAGCCAATCCAAACGGACCATTGTTCCATCAGGCTTTAAATATAAGTAAAAAAAGTACATGTATGTGGTTCAAATACATGTACTTTTTGCTTATGATTATTTTTAAGGCTGGAGGGAGTATCATATCATACTAGAGCTTATGCATAAGCTGTTTCTACATTTCAAGAGGGAATAAGCAATGTGGAGGAGCTTATGAACATAAGCTGAAAACAGTTCATGATCATATCATAATTCATAAACTATTTTCTTAAGCTCTTCCAAACAGTCATGCAAGTATTTATGACATAAGATAAACCCAAATGCATTTTTCAGGCTAGAATATAAGATTTTATTCTTGTTTATTGTTAAAGCTGATACTTTTTTATTTCCTTTTCTTAGGGAATTTCGGTCTTTTTGGTCGTGCCATTTCACAAGGTACTGTACTGTCGATATAACAGCAAGACTAAGATTCTTTCTAAAGTTTCTACATTTTGATTTCTGAAGGTTTTGTAATTATATTTAATTTGTAACTTCAATTTATGTGGGAAACTGAAGTAACTAGCTATTAATCTGTAGAGAAAGAAGATGGTCCAGAATATGCCCAACTTTCTGTGACTGTAAATGAAAATCTGCTCACAACTAATGGGGAGGTATTTAATTCAGATCAACTTCTCTTTTATAATTCCAAAAAGTCAATATGGTCAATCTCCTTTGAAGTCTGAACTCTAAAAAAGAATTAATCTAAGATTATAACTTCCCTGTTTGTTCTAATTTGACTTATATTCATATTCTGCAGTTTTCTTCTGAAAGAGGAGGTTCAGTTTCCGGAATATTGCATGAACTTCTTCAGAAGGGAGATGCAGCACAAAAGTACATGCAAGGATCTAGAAGCATGAAAATCGACAGCTATATCCTTCTTGATAATTTTGTGCAAGGACGTGCGCTTTCTTCATCTTCTCAGACAAGGGCTTTGCAAATTCATTCAAAACGTTCTAAGAGGCATATGTCCATGAAACAACATAAAAAACATGGATCAATGGATCTTCCCAAAGAGTTCCATAAGTAAGTTCAAGGTCTCTAAACTTACATTTTATGGAAGTATTTTGGTCCTTGTCATTACTACTATATCCCTGCAGTTTGTGGCAGTGAGAAATTTTTCATTTCGTCTTTCATTGGGATATTCTTAGATTCATGCTAGGAACAAATAAAGTGTTATACATTTTACTTTATTCTAATTGCTTAAATTTGTCTACAATGATATTATTTTGATGACCTAATCAGCAATCACGGTTCATTTTGATTACAGATTTGACATTTTCAAGCCAATGCATGACATGTGGAAAGACTATATAAAGTTGTTGCTTAAATCCACTGGGTACTGTTATTGTATATTTGCTATTGATGAATTTCACAGCTTCTTATCTTCGAGTTTTTCAGCACTTTTATTCTCTCACTGCTTTTATAATTATGACAACAAACCTGTTTATTGTTCCAGGAATAATCAATTGGCTCAATGTCTCCTAGGTGCAGATCTACATGGTGCTATTATTTTAGGTTTGAGCACACTCTATTTTTGTTTTGTTTTTGGATCAGTTGTGAACACCTACAGTAGAAAATGCTAAACCATTTTATTTATCTAGAGCTTTTTGGATAATTACAAGGACAGTTATTTTGTCATTGGTTGGATGTTAATTATTCCCACGGATGCTTATGTCTTTATGTGTAAATATTTCATATGCATAGAAAGGTGTGTTAAAATTGTCGTTGCCGCATGTACTCAAACCTGTGAAAGCCTACTTTTGGTGTTCTTAGAAAGAGTTAGAGTAGAACTATTTTACGCAATCATTGGTATTTAATGTTGCAACTTGCAATAAGTTCAATAGTAAGATGAGCTTCTGATTGGTTGAGGTCATACCTAGGTCTTCTTCTCCGGGAATGTTTCTCATTTAAATCCTTCCTTTTCATTCCCAGTGGCATGCATAACCTTTTTTCATGCTCCTTACACTTGGGATTTTTTTTTAAAAGTTGCTAGAACTAATGTTGAATGTTTTAGGTGTTTAATCGAATCAATTCTTTTAAAATGCATTTCTATTAGTGTTGTTTTAGTTTAATGTTATCATGTTGATAATTTTCTATATCCAAACTCTGTCTAGATGACTCAACATGCATTGTCCTTTGAATTTAGAAAAGAGTTGTGCTTTTGAGACTCCTTCAAAACAAATACAAATATTTTAGCCTCTACCTCCTAAATAATTGTCTGCTAGTTATCACTGACTGCACTATATCCACCTGTTATGCAGTTGTGGAGTGTAAACTAACCCATTTTACTGGAACTGGTGGCATCATGATTCGTGAAACTGCAGAAGCTTTTGGGATAATTACTGAAGCTAATAAATTCCAAGGTAATCCTTTCACTACTTAAAATAGAATCTGTTCTAGCATTACCAGAGCTGTTATTCCTGTGTTTCTCAAGTATTAATGCTTATTGAATATACACAGTCGTTTGTGTATGCTTTTCTTATACCAGGGTTTGCTGTACAAATTTGATAATTAATGAATTATAAGGAGTTTGCACCTCCGTTTTGAAAATTCAGCATAGTTTAAACAGTAATTGTTTTTGAATTTTTATATAAATTAAAGACTAATGACAAATAATCAATGTGTTCAGTTGCTTTTAAAATGGAACTTGTGGACATGGAAAAGTACAGCTTATATGTTTGAATGTAAAGGAAAATTTCTGTTTTGCTTAACTTGAGAAGATGTATTTTATGACAGGCATGGAATTTTCTGTTTCATGTTTCCGCTTTCTTGAGTAACAAGCAGATATGATAGTCATTCTTATAAACAATATGGCTGAGATTCTTATTTGGCACTATTAGATTGTCCTGTCAGGTTATATCTTTAGTATTCTGCAGAAACCAACCAGATTTGCTACATATAATTGTACGGAGATCTGAAGCTGTTTTAATGTGATTGTATATTTCCTATTGATTTTTTTTTTTACTCAGCTGTTTCTAATTTACGGTTGAATGAGTTCTCACTTTTTATTTAGGGTAGAATGCTCCAAAGTTTTAGTTAGATCAAAAACCTATTTTCTTTTCAAAATTCTTCCTCTTTTACTAAGAAAATGAAATATATACTTTGACTCGAAAGTCATTAATAGGTTCATATGTGACACTTGCATTATTATGGCTGAATTGGTTAAGAATTAGTGACCTTGAGCATCACAATTCTGTGTTCCATTTGCTTAAGTTGAAGTTTTGATGACTTATCAGTTCTGGTATATTTTTTCTCTAATATCTTTTCAAAAATGTGTTCACCAAATACTTTGATTTTTTAATTGCAGTTGTTCCGAAGAAGGGTTCTGTATTTGTACTACAAGTTGATTGCTGGAAAGCCACTCTGCTTGGAGATAAACTAGATTCAAGAAAGGTTGGATTATGA
Protein sequence

>MS.gene053583.t1

MAAESRKRAMEALERRIQTEHKLKEKKNKRDINHQHILKEKKIMKSINEDVKSPTPPPSTSNDPSLPLCRPSSDTPNKGNFGLFGRAISQEKEDGPEYAQLSVTVNENLLTTNGEFSSERGGSVSGILHELLQKGDAAQKYMQGSRSMKIDSYILLDNFVQGRALSSSSQTRALQIHSKRSKRHMSMKQHKKHGSMDLPKEFHKFDIFKPMHDMWKDYIKLLLKSTGNNQLAQCLLGADLHGAIILVVECKLTHFTGTGGIMIRETAEAFGIITEANKFQVVPKKGSVFVLQVDCWKATLLGDKLDSRKVGL