Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05906.t1 | KEH21354.1 | 95.4 | 173 | 8 | 0 | 1 | 173 | 1 | 173 | 4.00E-86 | 327.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05906.t1 | A0A072TV47 | 95.4 | 173 | 8 | 0 | 1 | 173 | 1 | 173 | 2.9e-86 | 327.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene05906 | MS.gene058121 | PPI |
| MS.gene05906 | MS.gene90225 | PPI |
| MS.gene05906 | MS.gene21881 | PPI |
| MS.gene05906 | MS.gene053583 | PPI |
| MS.gene05906 | MS.gene21743 | PPI |
| MS.gene05906 | MS.gene068020 | PPI |
| MS.gene05906 | MS.gene024760 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05906.t1 | MTR_8g103265 | 95.376 | 173 | 8 | 0 | 1 | 173 | 1 | 173 | 3.72e-121 | 339 |
| MS.gene05906.t1 | MTR_8g103265 | 95.322 | 171 | 8 | 0 | 3 | 173 | 52 | 222 | 3.48e-119 | 336 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05906.t1 | AT4G01790 | 48.936 | 141 | 60 | 1 | 1 | 141 | 38 | 166 | 1.32e-43 | 142 |
Find 39 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCCATTGCTGTTGGAATTA+AGG | 0.157846 | 4.4:+3113925 | MS.gene05906:CDS |
| TTTCACTGACTGCAGGTTAA+AGG | 0.230538 | 4.4:+3114435 | MS.gene05906:intron |
| TATTATCCTTAACAAATATC+AGG | 0.234820 | 4.4:-3113859 | None:intergenic |
| GACAAAGCATCTTCAAAGTT+TGG | 0.272288 | 4.4:+3113817 | MS.gene05906:CDS |
| TGCAGCAACAATTTGCTATT+GGG | 0.276797 | 4.4:+3113225 | MS.gene05906:intron |
| TTGCAGCAACAATTTGCTAT+TGG | 0.304157 | 4.4:+3113224 | MS.gene05906:intron |
| GTTCTTTGCCAGAAAAGATA+TGG | 0.347038 | 4.4:+3113093 | MS.gene05906:CDS |
| AAAGAAGGTTCTTTGAGATT+AGG | 0.350873 | 4.4:+3113881 | MS.gene05906:CDS |
| ACAAAGTTTCCCTGTTTGTC+AGG | 0.362507 | 4.4:-3114561 | None:intergenic |
| AACACCTTCAGTTAAACTTC+AGG | 0.377815 | 4.4:+3113343 | MS.gene05906:CDS |
| CCAGCTGAATTTGATTCATC+AGG | 0.390898 | 4.4:-3114516 | None:intergenic |
| TGTTCCCCTGATATTTGTTA+AGG | 0.407666 | 4.4:+3113853 | MS.gene05906:CDS |
| TTTGTTAAGGATAATAAAGA+AGG | 0.432172 | 4.4:+3113866 | MS.gene05906:CDS |
| GAATCAGCAAGGCATTTAGA+TGG | 0.437664 | 4.4:+3113068 | MS.gene05906:CDS |
| AACCGGTTATTCCGATGAGC+AGG | 0.471864 | 4.4:+3113039 | None:intergenic |
| ATTATCCTTAACAAATATCA+GGG | 0.488976 | 4.4:-3113858 | None:intergenic |
| TTGAAAACTGCCATTGCTGT+TGG | 0.496333 | 4.4:+3113917 | MS.gene05906:CDS |
| CAAAGTTTCCCTGTTTGTCA+GGG | 0.499070 | 4.4:-3114560 | None:intergenic |
| TTCAAAGTTTGGCTTCATCA+AGG | 0.506495 | 4.4:+3113828 | MS.gene05906:CDS |
| CCTGATGAATCAAATTCAGC+TGG | 0.516597 | 4.4:+3114516 | MS.gene05906:CDS |
| GCAGCAACAATTTGCTATTG+GGG | 0.528210 | 4.4:+3113226 | MS.gene05906:intron |
| AACCGTGTGCAGAGTTTGAA+AGG | 0.553964 | 4.4:+3113282 | MS.gene05906:CDS |
| TTATGATTGCTTGTGACTGA+AGG | 0.557148 | 4.4:-3113320 | None:intergenic |
| CGGTTATTCCGATGAGCAGG+GGG | 0.559631 | 4.4:+3113042 | None:intergenic |
| GCCAGAAAAGATATGGACAA+AGG | 0.562195 | 4.4:+3113100 | MS.gene05906:CDS |
| CGCTATTAATAAAATTGTCG+AGG | 0.562774 | 4.4:+3114461 | MS.gene05906:CDS |
| CACACGGTTTCATACGCTCT+AGG | 0.572427 | 4.4:-3113268 | None:intergenic |
| GACCTTTCAAACTCTGCACA+CGG | 0.575228 | 4.4:-3113284 | None:intergenic |
| AACCTTAATTCCAACAGCAA+TGG | 0.585166 | 4.4:-3113927 | None:intergenic |
| ACCGGTTATTCCGATGAGCA+GGG | 0.585921 | 4.4:+3113040 | None:intergenic |
| TATGATTGCTTGTGACTGAA+GGG | 0.589422 | 4.4:-3113319 | None:intergenic |
| TCAACCTGAAGTTTAACTGA+AGG | 0.601860 | 4.4:-3113347 | None:intergenic |
| TTTCAACACCCTGACAAACA+GGG | 0.614691 | 4.4:+3114552 | MS.gene05906:CDS |
| CTGAATTTGATTCATCAGGT+CGG | 0.627196 | 4.4:-3114512 | None:intergenic |
| CCGGTTATTCCGATGAGCAG+GGG | 0.633897 | 4.4:+3113041 | None:intergenic |
| TTATCCTTAACAAATATCAG+GGG | 0.650486 | 4.4:-3113857 | None:intergenic |
| CTTCTGACTGTAGCTCACGA+TGG | 0.681030 | 4.4:+3113792 | MS.gene05906:CDS |
| GATTGCTTGTGACTGAAGGG+AGG | 0.699866 | 4.4:-3113316 | None:intergenic |
| GGTTATTCCGATGAGCAGGG+GGG | 0.754880 | 4.4:+3113043 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTTTTTGTAAATTATTTC+GGG | + | chr4.4:3114093-3114112 | MS.gene05906:intron | 10.0% |
| !! | AAACTAGTTATTTATTACCA+AGG | - | chr4.4:3113643-3113662 | None:intergenic | 20.0% |
| !! | ACTTAGGTAAAAGTAATATA+TGG | + | chr4.4:3113670-3113689 | MS.gene05906:intron | 20.0% |
| !! | ATATTAAAAATAGAGTAAGC+TGG | - | chr4.4:3113462-3113481 | None:intergenic | 20.0% |
| !! | ATTATCCTTAACAAATATCA+GGG | - | chr4.4:3113861-3113880 | None:intergenic | 20.0% |
| !! | TATTATCCTTAACAAATATC+AGG | - | chr4.4:3113862-3113881 | None:intergenic | 20.0% |
| !! | TTTGTTAAGGATAATAAAGA+AGG | + | chr4.4:3113866-3113885 | MS.gene05906:CDS | 20.0% |
| !!! | ATTTTATTTTATGATGATGC+AGG | + | chr4.4:3113757-3113776 | MS.gene05906:intron | 20.0% |
| !!! | CATTCATATGTTTTATAGTT+AGG | - | chr4.4:3113146-3113165 | None:intergenic | 20.0% |
| !!! | TTAATATTTCTACCTTTACA+TGG | + | chr4.4:3113475-3113494 | MS.gene05906:intron | 20.0% |
| !!! | TTTCTTTTATTCTGATATGA+AGG | + | chr4.4:3113370-3113389 | MS.gene05906:intron | 20.0% |
| ! | AAACTGATATATAGAAATGC+AGG | - | chr4.4:3114194-3114213 | None:intergenic | 25.0% |
| ! | AACTCCTAGTATAATTTGAA+GGG | - | chr4.4:3113562-3113581 | None:intergenic | 25.0% |
| ! | CACAAGATATATATTCTGTT+TGG | + | chr4.4:3113964-3113983 | MS.gene05906:intron | 25.0% |
| ! | CAGATCTTATTAGCAAAATA+AGG | - | chr4.4:3114217-3114236 | None:intergenic | 25.0% |
| ! | GAATGAATTTAGCAATATGT+AGG | + | chr4.4:3113161-3113180 | MS.gene05906:intron | 25.0% |
| ! | TAGTTAGGAAAATCAAGTTA+AGG | - | chr4.4:3113131-3113150 | None:intergenic | 25.0% |
| ! | TGATTGTGATATTGAAAACT+AGG | - | chr4.4:3114153-3114172 | None:intergenic | 25.0% |
| ! | TTATCCTTAACAAATATCAG+GGG | - | chr4.4:3113860-3113879 | None:intergenic | 25.0% |
| !! | AACTAGTTTATTCTGTACTT+AGG | + | chr4.4:3113654-3113673 | MS.gene05906:intron | 25.0% |
| !! | GTGAATATATTTTCTCTTCA+AGG | - | chr4.4:3113737-3113756 | None:intergenic | 25.0% |
| !!! | ATAACCTTCTAAAGCTATTT+TGG | + | chr4.4:3114373-3114392 | MS.gene05906:intron | 25.0% |
| AAAAGCCTAACTATAACTAC+CGG | - | chr4.4:3113520-3113539 | None:intergenic | 30.0% | |
| ATAGTCATATAACGTTTACC+TGG | + | chr4.4:3113192-3113211 | MS.gene05906:intron | 30.0% | |
| CAACTCCTAGTATAATTTGA+AGG | - | chr4.4:3113563-3113582 | None:intergenic | 30.0% | |
| CAGAATATATATCTTGTGTG+AGG | - | chr4.4:3113962-3113981 | None:intergenic | 30.0% | |
| CGCTATTAATAAAATTGTCG+AGG | + | chr4.4:3114461-3114480 | MS.gene05906:CDS | 30.0% | |
| GACACATTAAACTCAAGAAA+TGG | - | chr4.4:3114257-3114276 | None:intergenic | 30.0% | |
| TGAAAACTAGGATTCAACAA+GGG | - | chr4.4:3114141-3114160 | None:intergenic | 30.0% | |
| TGATCCCTTCAAATTATACT+AGG | + | chr4.4:3113555-3113574 | MS.gene05906:intron | 30.0% | |
| TTAAAAATAGAGTAAGCTGG+AGG | - | chr4.4:3113459-3113478 | None:intergenic | 30.0% | |
| TTCTTGAGTTTAATGTGTCA+TGG | + | chr4.4:3114258-3114277 | MS.gene05906:intron | 30.0% | |
| TTGAAAACTAGGATTCAACA+AGG | - | chr4.4:3114142-3114161 | None:intergenic | 30.0% | |
| ! | ACTAACTGTGTTTGAAATTG+TGG | - | chr4.4:3113617-3113636 | None:intergenic | 30.0% |
| !! | AAAGAAGGTTCTTTGAGATT+AGG | + | chr4.4:3113881-3113900 | MS.gene05906:CDS | 30.0% |
| !! | GAAACCAAAATAGCTTTAGA+AGG | - | chr4.4:3114380-3114399 | None:intergenic | 30.0% |
| AAATGCAGGATTGAAAAGCA+AGG | - | chr4.4:3114180-3114199 | None:intergenic | 35.0% | |
| AACACCTTCAGTTAAACTTC+AGG | + | chr4.4:3113343-3113362 | MS.gene05906:CDS | 35.0% | |
| AACCTTAATTCCAACAGCAA+TGG | - | chr4.4:3113930-3113949 | None:intergenic | 35.0% | |
| AAGTAATATATGGCTGCTTC+TGG | + | chr4.4:3113680-3113699 | MS.gene05906:intron | 35.0% | |
| AATAGCAAATTGTTGCTGCA+AGG | - | chr4.4:3113225-3113244 | None:intergenic | 35.0% | |
| AATGCAGGATTGAAAAGCAA+GGG | - | chr4.4:3114179-3114198 | None:intergenic | 35.0% | |
| AATTGTGGATGCGAAAACAA+TGG | - | chr4.4:3113602-3113621 | None:intergenic | 35.0% | |
| CTGAATTTGATTCATCAGGT+CGG | - | chr4.4:3114515-3114534 | None:intergenic | 35.0% | |
| GTTCTTTGCCAGAAAAGATA+TGG | + | chr4.4:3113093-3113112 | MS.gene05906:CDS | 35.0% | |
| TATGATTGCTTGTGACTGAA+GGG | - | chr4.4:3113322-3113341 | None:intergenic | 35.0% | |
| TCAACCTGAAGTTTAACTGA+AGG | - | chr4.4:3113350-3113369 | None:intergenic | 35.0% | |
| TTATGATTGCTTGTGACTGA+AGG | - | chr4.4:3113323-3113342 | None:intergenic | 35.0% | |
| TTCAACAAGGGAAGCTATTA+TGG | - | chr4.4:3114129-3114148 | None:intergenic | 35.0% | |
| TTGGAATTAAGGTTAGATCC+TGG | + | chr4.4:3113936-3113955 | MS.gene05906:intron | 35.0% | |
| ! | ACCTTTGTCCATATCTTTTC+TGG | - | chr4.4:3113104-3113123 | None:intergenic | 35.0% |
| ! | ATTTATCTTTCACTGACTGC+AGG | + | chr4.4:3114428-3114447 | MS.gene05906:intron | 35.0% |
| ! | GACAAAGCATCTTCAAAGTT+TGG | + | chr4.4:3113817-3113836 | MS.gene05906:CDS | 35.0% |
| ! | GTATTTGAAGCACCATGTAA+AGG | - | chr4.4:3113490-3113509 | None:intergenic | 35.0% |
| ! | TGTTCCCCTGATATTTGTTA+AGG | + | chr4.4:3113853-3113872 | MS.gene05906:CDS | 35.0% |
| ! | TTCAAAGTTTGGCTTCATCA+AGG | + | chr4.4:3113828-3113847 | MS.gene05906:CDS | 35.0% |
| !! | AGAAGAATTCAAGAGTGTCA+TGG | + | chr4.4:3114283-3114302 | MS.gene05906:intron | 35.0% |
| !! | TGCAGCAACAATTTGCTATT+GGG | + | chr4.4:3113225-3113244 | MS.gene05906:intron | 35.0% |
| !! | TTGCAGCAACAATTTGCTAT+TGG | + | chr4.4:3113224-3113243 | MS.gene05906:intron | 35.0% |
| !!! | CAAACACAGTTAGTTTTCCT+TGG | + | chr4.4:3113623-3113642 | MS.gene05906:intron | 35.0% |
| AACTATAACTACCGGTGCAT+GGG | - | chr4.4:3113512-3113531 | None:intergenic | 40.0% | |
| AAGCCATGGTCAAATAACAC+GGG | - | chr4.4:3114342-3114361 | None:intergenic | 40.0% | |
| ACAAAGTTTCCCTGTTTGTC+AGG | - | chr4.4:3114564-3114583 | None:intergenic | 40.0% | |
| CAAAGTTTCCCTGTTTGTCA+GGG | - | chr4.4:3114563-3114582 | None:intergenic | 40.0% | |
| CAAGCCATGGTCAAATAACA+CGG | - | chr4.4:3114343-3114362 | None:intergenic | 40.0% | |
| CATATAACGTTTACCTGGAC+TGG | + | chr4.4:3113197-3113216 | MS.gene05906:intron | 40.0% | |
| CCAGCTGAATTTGATTCATC+AGG | - | chr4.4:3114519-3114538 | None:intergenic | 40.0% | |
| CCTGATGAATCAAATTCAGC+TGG | + | chr4.4:3114516-3114535 | MS.gene05906:CDS | 40.0% | |
| GAACTAGTAACAACAGGTAC+AGG | - | chr4.4:3113419-3113438 | None:intergenic | 40.0% | |
| GAAGAATTCAAGAGTCCAAC+AGG | + | chr4.4:3114309-3114328 | MS.gene05906:intron | 40.0% | |
| GCCAGAAAAGATATGGACAA+AGG | + | chr4.4:3113100-3113119 | MS.gene05906:CDS | 40.0% | |
| TAACTATAACTACCGGTGCA+TGG | - | chr4.4:3113513-3113532 | None:intergenic | 40.0% | |
| TATAGTGACATCTCAAGCCA+TGG | - | chr4.4:3114356-3114375 | None:intergenic | 40.0% | |
| TATATATCTTGTGTGAGGCC+AGG | - | chr4.4:3113957-3113976 | None:intergenic | 40.0% | |
| TGCGATGAACTAGTAACAAC+AGG | - | chr4.4:3113425-3113444 | None:intergenic | 40.0% | |
| TTGAAAACTGCCATTGCTGT+TGG | + | chr4.4:3113917-3113936 | MS.gene05906:CDS | 40.0% | |
| TTTCAACACCCTGACAAACA+GGG | + | chr4.4:3114552-3114571 | MS.gene05906:CDS | 40.0% | |
| ! | ATGCACCGGTAGTTATAGTT+AGG | + | chr4.4:3113512-3113531 | MS.gene05906:intron | 40.0% |
| ! | GAATCAGCAAGGCATTTAGA+TGG | + | chr4.4:3113068-3113087 | MS.gene05906:CDS | 40.0% |
| ! | TGCCATTGCTGTTGGAATTA+AGG | + | chr4.4:3113925-3113944 | MS.gene05906:CDS | 40.0% |
| ! | TTTCACTGACTGCAGGTTAA+AGG | + | chr4.4:3114435-3114454 | MS.gene05906:intron | 40.0% |
| ! | TTTTCAACACCCTGACAAAC+AGG | + | chr4.4:3114551-3114570 | MS.gene05906:CDS | 40.0% |
| !! | GCAGCAACAATTTGCTATTG+GGG | + | chr4.4:3113226-3113245 | MS.gene05906:intron | 40.0% |
| AACCGTGTGCAGAGTTTGAA+AGG | + | chr4.4:3113282-3113301 | MS.gene05906:CDS | 45.0% | |
| ATATATGGCTGCTTCTGGTG+TGG | + | chr4.4:3113685-3113704 | MS.gene05906:intron | 45.0% | |
| CTAGGAGTTGACAGTGTGTA+TGG | + | chr4.4:3113573-3113592 | MS.gene05906:intron | 45.0% | |
| GACCTTTCAAACTCTGCACA+CGG | - | chr4.4:3113287-3113306 | None:intergenic | 45.0% | |
| TATATGGCTGCTTCTGGTGT+GGG | + | chr4.4:3113686-3113705 | MS.gene05906:intron | 45.0% | |
| TGCTTCAAATACCCATGCAC+CGG | + | chr4.4:3113498-3113517 | MS.gene05906:intron | 45.0% | |
| !! | GAACCCGTGTTATTTGACCA+TGG | + | chr4.4:3114336-3114355 | MS.gene05906:intron | 45.0% |
| !!! | TTATTTTTTGTAAATTATTT+CGG | + | chr4.4:3114092-3114111 | MS.gene05906:intron | 5.0% |
| CACACGGTTTCATACGCTCT+AGG | - | chr4.4:3113271-3113290 | None:intergenic | 50.0% | |
| CTTCTGACTGTAGCTCACGA+TGG | + | chr4.4:3113792-3113811 | MS.gene05906:CDS | 50.0% | |
| GATTGCTTGTGACTGAAGGG+AGG | - | chr4.4:3113319-3113338 | None:intergenic | 50.0% | |
| AACACGGGTTCGTTGCCTGT+TGG | - | chr4.4:3114327-3114346 | None:intergenic | 55.0% | |
| TTGCTGCAAGGTTCCAGTCC+AGG | - | chr4.4:3113213-3113232 | None:intergenic | 55.0% | |
| GCAGGGGGGTTGAATCAGCA+AGG | + | chr4.4:3113057-3113076 | MS.gene05906:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 3113053 | 3114599 | 3113053 | ID=MS.gene05906 |
| chr4.4 | mRNA | 3113053 | 3114599 | 3113053 | ID=MS.gene05906.t1;Parent=MS.gene05906 |
| chr4.4 | exon | 3113053 | 3113121 | 3113053 | ID=MS.gene05906.t1.exon1;Parent=MS.gene05906.t1 |
| chr4.4 | CDS | 3113053 | 3113121 | 3113053 | ID=cds.MS.gene05906.t1;Parent=MS.gene05906.t1 |
| chr4.4 | exon | 3113230 | 3113364 | 3113230 | ID=MS.gene05906.t1.exon2;Parent=MS.gene05906.t1 |
| chr4.4 | CDS | 3113230 | 3113364 | 3113230 | ID=cds.MS.gene05906.t1;Parent=MS.gene05906.t1 |
| chr4.4 | exon | 3113779 | 3113946 | 3113779 | ID=MS.gene05906.t1.exon3;Parent=MS.gene05906.t1 |
| chr4.4 | CDS | 3113779 | 3113946 | 3113779 | ID=cds.MS.gene05906.t1;Parent=MS.gene05906.t1 |
| chr4.4 | exon | 3114450 | 3114599 | 3114450 | ID=MS.gene05906.t1.exon4;Parent=MS.gene05906.t1 |
| chr4.4 | CDS | 3114450 | 3114599 | 3114450 | ID=cds.MS.gene05906.t1;Parent=MS.gene05906.t1 |
| Gene Sequence |
| Protein sequence |
