Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049112.t1 | KEH21354.1 | 94.8 | 173 | 9 | 0 | 1 | 173 | 1 | 173 | 1.50E-85 | 325.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049112.t1 | A0A072TV47 | 94.8 | 173 | 9 | 0 | 1 | 173 | 1 | 173 | 1.1e-85 | 325.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene049112 | MS.gene90225 | PPI |
| MS.gene21743 | MS.gene049112 | PPI |
| MS.gene049112 | MS.gene053583 | PPI |
| MS.gene049112 | MS.gene058121 | PPI |
| MS.gene21881 | MS.gene049112 | PPI |
| MS.gene024760 | MS.gene049112 | PPI |
| MS.gene068020 | MS.gene049112 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049112.t1 | MTR_8g103265 | 94.798 | 173 | 9 | 0 | 1 | 173 | 1 | 173 | 2.54e-120 | 337 |
| MS.gene049112.t1 | MTR_8g103265 | 94.737 | 171 | 9 | 0 | 3 | 173 | 52 | 222 | 4.05e-118 | 333 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049112.t1 | AT4G01790 | 48.936 | 141 | 60 | 1 | 1 | 141 | 38 | 166 | 2.43e-43 | 142 |
Find 40 sgRNAs with CRISPR-Local
Find 99 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCCATTGCTGTTGGAATTA+AGG | 0.157846 | 4.1:+2784850 | MS.gene049112:CDS |
| TTTCACTGACTGCAGGTTAA+AGG | 0.230538 | 4.1:+2785361 | MS.gene049112:intron |
| TATTATCCTTAACAAATATC+AGG | 0.234820 | 4.1:-2784784 | None:intergenic |
| GACAAAGCATCTTCAAAGTT+TGG | 0.272288 | 4.1:+2784742 | MS.gene049112:CDS |
| TGCAGCAACAATTTGCTATT+GGG | 0.276797 | 4.1:+2784150 | MS.gene049112:intron |
| TTGCAGCAACAATTTGCTAT+TGG | 0.304157 | 4.1:+2784149 | MS.gene049112:intron |
| GTTCTTTGCCAGAAAAGATA+TGG | 0.347038 | 4.1:+2784018 | MS.gene049112:CDS |
| AAAGAAGGTTCTTTGAGATT+AGG | 0.350873 | 4.1:+2784806 | MS.gene049112:CDS |
| ACAAAGTTTCCCTGTTTGTC+AGG | 0.362507 | 4.1:-2785487 | None:intergenic |
| AACACCTTCAGTTAAACTTC+AGG | 0.377815 | 4.1:+2784268 | MS.gene049112:CDS |
| CCAGCTGAATTTGATTCATC+AGG | 0.390898 | 4.1:-2785442 | None:intergenic |
| TGTTCCCCTGATATTTGTTA+AGG | 0.407666 | 4.1:+2784778 | MS.gene049112:CDS |
| TTTGTTAAGGATAATAAAGA+AGG | 0.432172 | 4.1:+2784791 | MS.gene049112:CDS |
| GAATCAGCAAGGCATTTAGA+TGG | 0.437664 | 4.1:+2783993 | MS.gene049112:CDS |
| AACCGGTTATTCCGATGAGC+AGG | 0.471864 | 4.1:+2783964 | None:intergenic |
| ATTATCCTTAACAAATATCA+GGG | 0.488976 | 4.1:-2784783 | None:intergenic |
| TTGAAAACTGCCATTGCTGT+TGG | 0.496333 | 4.1:+2784842 | MS.gene049112:CDS |
| CAAAGTTTCCCTGTTTGTCA+GGG | 0.499070 | 4.1:-2785486 | None:intergenic |
| TTCAAAGTTTGGCTTCATCA+AGG | 0.506495 | 4.1:+2784753 | MS.gene049112:CDS |
| CCTGATGAATCAAATTCAGC+TGG | 0.516597 | 4.1:+2785442 | MS.gene049112:CDS |
| TGAGCTGACCTTTCAAACTC+TGG | 0.516741 | 4.1:-2784215 | None:intergenic |
| GCAGCAACAATTTGCTATTG+GGG | 0.528210 | 4.1:+2784151 | MS.gene049112:intron |
| GACCTTTCAAACTCTGGACA+TGG | 0.549650 | 4.1:-2784209 | None:intergenic |
| TTATGATTGCTTGTGACTGA+AGG | 0.557148 | 4.1:-2784245 | None:intergenic |
| AACCATGTCCAGAGTTTGAA+AGG | 0.559391 | 4.1:+2784207 | MS.gene049112:CDS |
| CGGTTATTCCGATGAGCAGG+GGG | 0.559631 | 4.1:+2783967 | None:intergenic |
| GCCAGAAAAGATATGGACAA+AGG | 0.562195 | 4.1:+2784025 | MS.gene049112:CDS |
| CGCTATTAATAAAATTGTCG+AGG | 0.562774 | 4.1:+2785387 | MS.gene049112:CDS |
| AACCTTAATTCCAACAGCAA+TGG | 0.585166 | 4.1:-2784852 | None:intergenic |
| ACCGGTTATTCCGATGAGCA+GGG | 0.585921 | 4.1:+2783965 | None:intergenic |
| TATGATTGCTTGTGACTGAA+GGG | 0.589422 | 4.1:-2784244 | None:intergenic |
| TCAACCTGAAGTTTAACTGA+AGG | 0.601860 | 4.1:-2784272 | None:intergenic |
| GACATGGTTTCATACGCTCT+AGG | 0.610322 | 4.1:-2784193 | None:intergenic |
| TTTCAACACCCTGACAAACA+GGG | 0.614691 | 4.1:+2785478 | MS.gene049112:CDS |
| CTGAATTTGATTCATCAGGT+CGG | 0.627196 | 4.1:-2785438 | None:intergenic |
| CCGGTTATTCCGATGAGCAG+GGG | 0.633897 | 4.1:+2783966 | None:intergenic |
| TTATCCTTAACAAATATCAG+GGG | 0.650486 | 4.1:-2784782 | None:intergenic |
| CTTCTGACTGTAGCTCACGA+TGG | 0.681030 | 4.1:+2784717 | MS.gene049112:CDS |
| GATTGCTTGTGACTGAAGGG+AGG | 0.699866 | 4.1:-2784241 | None:intergenic |
| GGTTATTCCGATGAGCAGGG+GGG | 0.754880 | 4.1:+2783968 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTTGTAAATTATTTC+GGG | + | chr4.1:2785019-2785038 | MS.gene049112:intron | 10.0% |
| !! | AAACTAGTTATTTATTACCA+AGG | - | chr4.1:2784568-2784587 | None:intergenic | 20.0% |
| !! | ATATTAAAAATAGAGTAAGC+TGG | - | chr4.1:2784387-2784406 | None:intergenic | 20.0% |
| !! | ATTATCCTTAACAAATATCA+GGG | - | chr4.1:2784786-2784805 | None:intergenic | 20.0% |
| !! | TATTATCCTTAACAAATATC+AGG | - | chr4.1:2784787-2784806 | None:intergenic | 20.0% |
| !! | TTTGTTAAGGATAATAAAGA+AGG | + | chr4.1:2784791-2784810 | MS.gene049112:CDS | 20.0% |
| !!! | ATTTTATTTTATGATGATGC+AGG | + | chr4.1:2784682-2784701 | MS.gene049112:intron | 20.0% |
| !!! | CATTCATATGTTTTATAGTT+AGG | - | chr4.1:2784071-2784090 | None:intergenic | 20.0% |
| !!! | TTAATATTTCTACCTTTACA+TGG | + | chr4.1:2784400-2784419 | MS.gene049112:intron | 20.0% |
| !!! | TTTCTTTTATTCTGATATGA+AGG | + | chr4.1:2784295-2784314 | MS.gene049112:intron | 20.0% |
| ! | AAACTGATATATAGAAATGC+AGG | - | chr4.1:2785120-2785139 | None:intergenic | 25.0% |
| ! | ACTTGGGTAAAAGTAATATA+TGG | + | chr4.1:2784595-2784614 | MS.gene049112:intron | 25.0% |
| ! | CACAAGATATATATTCTGTT+TGG | + | chr4.1:2784889-2784908 | MS.gene049112:intron | 25.0% |
| ! | CAGATCTTATTAGCAAAATA+AGG | - | chr4.1:2785143-2785162 | None:intergenic | 25.0% |
| ! | GAATGAATTTAGCAATATGT+AGG | + | chr4.1:2784086-2784105 | MS.gene049112:intron | 25.0% |
| ! | TAGTTAGGAAAATCAAGTTA+AGG | - | chr4.1:2784056-2784075 | None:intergenic | 25.0% |
| ! | TGATTGTGATATTGAAAACT+AGG | - | chr4.1:2785079-2785098 | None:intergenic | 25.0% |
| ! | TTATCCTTAACAAATATCAG+GGG | - | chr4.1:2784785-2784804 | None:intergenic | 25.0% |
| !! | AACTAGTTTATTCTGTACTT+GGG | + | chr4.1:2784579-2784598 | MS.gene049112:intron | 25.0% |
| !! | GTGAATATATTTTCTCTTCA+AGG | - | chr4.1:2784662-2784681 | None:intergenic | 25.0% |
| !! | TAACTAGTTTATTCTGTACT+TGG | + | chr4.1:2784578-2784597 | MS.gene049112:intron | 25.0% |
| !!! | ATAACCTTCTAAAGCTATTT+TGG | + | chr4.1:2785299-2785318 | MS.gene049112:intron | 25.0% |
| AAAAGCCTAACTATAACTAC+CGG | - | chr4.1:2784445-2784464 | None:intergenic | 30.0% | |
| ATAGTCATATAACGTTTACC+TGG | + | chr4.1:2784117-2784136 | MS.gene049112:intron | 30.0% | |
| CAGAATATATATCTTGTGTG+AGG | - | chr4.1:2784887-2784906 | None:intergenic | 30.0% | |
| CGCTATTAATAAAATTGTCG+AGG | + | chr4.1:2785387-2785406 | MS.gene049112:CDS | 30.0% | |
| GACACATTAAACTCAAGAAA+TGG | - | chr4.1:2785183-2785202 | None:intergenic | 30.0% | |
| TGAAAACTAGGATTCATCAA+GGG | - | chr4.1:2785067-2785086 | None:intergenic | 30.0% | |
| TTAAAAATAGAGTAAGCTGG+AGG | - | chr4.1:2784384-2784403 | None:intergenic | 30.0% | |
| TTCATCAAGGGAAGTTATTA+TGG | - | chr4.1:2785055-2785074 | None:intergenic | 30.0% | |
| TTCTTGAGTTTAATGTGTCA+TGG | + | chr4.1:2785184-2785203 | MS.gene049112:intron | 30.0% | |
| TTGAAAACTAGGATTCATCA+AGG | - | chr4.1:2785068-2785087 | None:intergenic | 30.0% | |
| ! | AACTCCTAGTATAATTTGCA+GGG | - | chr4.1:2784487-2784506 | None:intergenic | 30.0% |
| ! | ACTAACTGTGTTTGAAATTG+TGG | - | chr4.1:2784542-2784561 | None:intergenic | 30.0% |
| !! | AAAGAAGGTTCTTTGAGATT+AGG | + | chr4.1:2784806-2784825 | MS.gene049112:CDS | 30.0% |
| !! | GAAACCAAAATAGCTTTAGA+AGG | - | chr4.1:2785306-2785325 | None:intergenic | 30.0% |
| AAATGCAGGATTGAAAAGCA+AGG | - | chr4.1:2785106-2785125 | None:intergenic | 35.0% | |
| AACACCTTCAGTTAAACTTC+AGG | + | chr4.1:2784268-2784287 | MS.gene049112:CDS | 35.0% | |
| AACCTTAATTCCAACAGCAA+TGG | - | chr4.1:2784855-2784874 | None:intergenic | 35.0% | |
| AACTATAACTACCGGTACAT+GGG | - | chr4.1:2784437-2784456 | None:intergenic | 35.0% | |
| AAGTAATATATGGCTGCTTC+TGG | + | chr4.1:2784605-2784624 | MS.gene049112:intron | 35.0% | |
| AATAGCAAATTGTTGCTGCA+AGG | - | chr4.1:2784150-2784169 | None:intergenic | 35.0% | |
| AATGCAGGATTGAAAAGCAA+GGG | - | chr4.1:2785105-2785124 | None:intergenic | 35.0% | |
| AATTGTGGATGCGAAAACAA+TGG | - | chr4.1:2784527-2784546 | None:intergenic | 35.0% | |
| CTGAATTTGATTCATCAGGT+CGG | - | chr4.1:2785441-2785460 | None:intergenic | 35.0% | |
| GTTCTTTGCCAGAAAAGATA+TGG | + | chr4.1:2784018-2784037 | MS.gene049112:CDS | 35.0% | |
| TAACTATAACTACCGGTACA+TGG | - | chr4.1:2784438-2784457 | None:intergenic | 35.0% | |
| TATGATTGCTTGTGACTGAA+GGG | - | chr4.1:2784247-2784266 | None:intergenic | 35.0% | |
| TCAACCTGAAGTTTAACTGA+AGG | - | chr4.1:2784275-2784294 | None:intergenic | 35.0% | |
| TGATCCCTGCAAATTATACT+AGG | + | chr4.1:2784480-2784499 | MS.gene049112:intron | 35.0% | |
| TTATGATTGCTTGTGACTGA+AGG | - | chr4.1:2784248-2784267 | None:intergenic | 35.0% | |
| TTGGAATTAAGGTTAGATCC+TGG | + | chr4.1:2784861-2784880 | MS.gene049112:intron | 35.0% | |
| ! | ACCTTTGTCCATATCTTTTC+TGG | - | chr4.1:2784029-2784048 | None:intergenic | 35.0% |
| ! | ATGTACCGGTAGTTATAGTT+AGG | + | chr4.1:2784437-2784456 | MS.gene049112:intron | 35.0% |
| ! | ATTTATCTTTCACTGACTGC+AGG | + | chr4.1:2785354-2785373 | MS.gene049112:intron | 35.0% |
| ! | CAACTCCTAGTATAATTTGC+AGG | - | chr4.1:2784488-2784507 | None:intergenic | 35.0% |
| ! | GACAAAGCATCTTCAAAGTT+TGG | + | chr4.1:2784742-2784761 | MS.gene049112:CDS | 35.0% |
| ! | GTATTTGAAGCACCATGTAA+AGG | - | chr4.1:2784415-2784434 | None:intergenic | 35.0% |
| ! | TGTTCCCCTGATATTTGTTA+AGG | + | chr4.1:2784778-2784797 | MS.gene049112:CDS | 35.0% |
| ! | TTCAAAGTTTGGCTTCATCA+AGG | + | chr4.1:2784753-2784772 | MS.gene049112:CDS | 35.0% |
| !! | AGAAGAATTCAAGAGTGTCA+TGG | + | chr4.1:2785209-2785228 | MS.gene049112:intron | 35.0% |
| !! | TGCAGCAACAATTTGCTATT+GGG | + | chr4.1:2784150-2784169 | MS.gene049112:intron | 35.0% |
| !! | TTGCAGCAACAATTTGCTAT+TGG | + | chr4.1:2784149-2784168 | MS.gene049112:intron | 35.0% |
| !!! | CAAACACAGTTAGTTTTCCT+TGG | + | chr4.1:2784548-2784567 | MS.gene049112:intron | 35.0% |
| AACCATGTCCAGAGTTTGAA+AGG | + | chr4.1:2784207-2784226 | MS.gene049112:CDS | 40.0% | |
| AAGCCATGGTCAAATAACAC+GGG | - | chr4.1:2785268-2785287 | None:intergenic | 40.0% | |
| ACAAAGTTTCCCTGTTTGTC+AGG | - | chr4.1:2785490-2785509 | None:intergenic | 40.0% | |
| CAAAGTTTCCCTGTTTGTCA+GGG | - | chr4.1:2785489-2785508 | None:intergenic | 40.0% | |
| CAAGCCATGGTCAAATAACA+CGG | - | chr4.1:2785269-2785288 | None:intergenic | 40.0% | |
| CATATAACGTTTACCTGGAC+TGG | + | chr4.1:2784122-2784141 | MS.gene049112:intron | 40.0% | |
| CCAGCTGAATTTGATTCATC+AGG | - | chr4.1:2785445-2785464 | None:intergenic | 40.0% | |
| CCTGATGAATCAAATTCAGC+TGG | + | chr4.1:2785442-2785461 | MS.gene049112:CDS | 40.0% | |
| GAACTAGTAACAACAGGTAC+AGG | - | chr4.1:2784344-2784363 | None:intergenic | 40.0% | |
| GAAGAATTCAAGAGTCCAAC+AGG | + | chr4.1:2785235-2785254 | MS.gene049112:intron | 40.0% | |
| GCCAGAAAAGATATGGACAA+AGG | + | chr4.1:2784025-2784044 | MS.gene049112:CDS | 40.0% | |
| TATAGTGACATCTCAAGCCA+TGG | - | chr4.1:2785282-2785301 | None:intergenic | 40.0% | |
| TATATATCTTGTGTGAGGCC+AGG | - | chr4.1:2784882-2784901 | None:intergenic | 40.0% | |
| TGCGATGAACTAGTAACAAC+AGG | - | chr4.1:2784350-2784369 | None:intergenic | 40.0% | |
| TGCTTCAAATACCCATGTAC+CGG | + | chr4.1:2784423-2784442 | MS.gene049112:intron | 40.0% | |
| TTGAAAACTGCCATTGCTGT+TGG | + | chr4.1:2784842-2784861 | MS.gene049112:CDS | 40.0% | |
| TTTCAACACCCTGACAAACA+GGG | + | chr4.1:2785478-2785497 | MS.gene049112:CDS | 40.0% | |
| ! | GAATCAGCAAGGCATTTAGA+TGG | + | chr4.1:2783993-2784012 | MS.gene049112:CDS | 40.0% |
| ! | TGCCATTGCTGTTGGAATTA+AGG | + | chr4.1:2784850-2784869 | MS.gene049112:CDS | 40.0% |
| ! | TTTCACTGACTGCAGGTTAA+AGG | + | chr4.1:2785361-2785380 | MS.gene049112:intron | 40.0% |
| ! | TTTTCAACACCCTGACAAAC+AGG | + | chr4.1:2785477-2785496 | MS.gene049112:CDS | 40.0% |
| !! | GCAGCAACAATTTGCTATTG+GGG | + | chr4.1:2784151-2784170 | MS.gene049112:intron | 40.0% |
| ATATATGGCTGCTTCTGGTG+TGG | + | chr4.1:2784610-2784629 | MS.gene049112:intron | 45.0% | |
| CTAGGAGTTGACAGTGTGTA+TGG | + | chr4.1:2784498-2784517 | MS.gene049112:intron | 45.0% | |
| GACATGGTTTCATACGCTCT+AGG | - | chr4.1:2784196-2784215 | None:intergenic | 45.0% | |
| GACCTTTCAAACTCTGGACA+TGG | - | chr4.1:2784212-2784231 | None:intergenic | 45.0% | |
| TATATGGCTGCTTCTGGTGT+GGG | + | chr4.1:2784611-2784630 | MS.gene049112:intron | 45.0% | |
| TGAGCTGACCTTTCAAACTC+TGG | - | chr4.1:2784218-2784237 | None:intergenic | 45.0% | |
| !! | GAACCCGTGTTATTTGACCA+TGG | + | chr4.1:2785262-2785281 | MS.gene049112:intron | 45.0% |
| !!! | TTTTTTTTTGTAAATTATTT+CGG | + | chr4.1:2785018-2785037 | MS.gene049112:intron | 5.0% |
| CTTCTGACTGTAGCTCACGA+TGG | + | chr4.1:2784717-2784736 | MS.gene049112:CDS | 50.0% | |
| GATTGCTTGTGACTGAAGGG+AGG | - | chr4.1:2784244-2784263 | None:intergenic | 50.0% | |
| AACACGGGTTCGTTGCCTGT+TGG | - | chr4.1:2785253-2785272 | None:intergenic | 55.0% | |
| TTGCTGCAAGGTTCCAGTCC+AGG | - | chr4.1:2784138-2784157 | None:intergenic | 55.0% | |
| GCAGGGGGGTTGAATCAGCA+AGG | + | chr4.1:2783982-2784001 | MS.gene049112:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 2783978 | 2785525 | 2783978 | ID=MS.gene049112 |
| chr4.1 | mRNA | 2783978 | 2785525 | 2783978 | ID=MS.gene049112.t1;Parent=MS.gene049112 |
| chr4.1 | exon | 2783978 | 2784046 | 2783978 | ID=MS.gene049112.t1.exon1;Parent=MS.gene049112.t1 |
| chr4.1 | CDS | 2783978 | 2784046 | 2783978 | ID=cds.MS.gene049112.t1;Parent=MS.gene049112.t1 |
| chr4.1 | exon | 2784155 | 2784289 | 2784155 | ID=MS.gene049112.t1.exon2;Parent=MS.gene049112.t1 |
| chr4.1 | CDS | 2784155 | 2784289 | 2784155 | ID=cds.MS.gene049112.t1;Parent=MS.gene049112.t1 |
| chr4.1 | exon | 2784704 | 2784871 | 2784704 | ID=MS.gene049112.t1.exon3;Parent=MS.gene049112.t1 |
| chr4.1 | CDS | 2784704 | 2784871 | 2784704 | ID=cds.MS.gene049112.t1;Parent=MS.gene049112.t1 |
| chr4.1 | exon | 2785376 | 2785525 | 2785376 | ID=MS.gene049112.t1.exon4;Parent=MS.gene049112.t1 |
| chr4.1 | CDS | 2785376 | 2785525 | 2785376 | ID=cds.MS.gene049112.t1;Parent=MS.gene049112.t1 |
| Gene Sequence |
| Protein sequence |
