Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053778.t1 | RHN55952.1 | 92.3 | 439 | 33 | 1 | 5 | 443 | 3 | 440 | 2.30E-234 | 821.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053778.t1 | Q99JW2 | 42.2 | 410 | 221 | 6 | 30 | 437 | 5 | 400 | 3.7e-92 | 340.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053778.t1 | A0A396HRI7 | 92.3 | 439 | 33 | 1 | 5 | 443 | 3 | 440 | 1.6e-234 | 821.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene053778 | MS.gene91126 | PPI |
| MS.gene053778 | MS.gene88610 | PPI |
| MS.gene053778 | MS.gene026174 | PPI |
| MS.gene053778 | MS.gene29220 | PPI |
| MS.gene053778 | MS.gene91122 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053778.t1 | MTR_5g058430 | 95.455 | 352 | 16 | 0 | 92 | 443 | 4 | 355 | 0.0 | 682 |
| MS.gene053778.t1 | MTR_1g039010 | 54.034 | 409 | 187 | 1 | 32 | 440 | 28 | 435 | 2.99e-166 | 475 |
| MS.gene053778.t1 | MTR_1g038920 | 54.208 | 404 | 184 | 1 | 37 | 440 | 36 | 438 | 4.29e-163 | 467 |
| MS.gene053778.t1 | MTR_1g038920 | 53.808 | 407 | 184 | 2 | 37 | 440 | 36 | 441 | 2.93e-161 | 462 |
| MS.gene053778.t1 | MTR_0400s0010 | 55.932 | 177 | 75 | 1 | 241 | 414 | 1 | 177 | 2.94e-70 | 220 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053778.t1 | AT1G44820 | 67.447 | 427 | 136 | 2 | 12 | 438 | 7 | 430 | 0.0 | 620 |
| MS.gene053778.t1 | AT1G44180 | 66.047 | 430 | 145 | 1 | 12 | 441 | 7 | 435 | 0.0 | 615 |
| MS.gene053778.t1 | AT1G44180 | 66.047 | 430 | 145 | 1 | 12 | 441 | 7 | 435 | 0.0 | 615 |
| MS.gene053778.t1 | AT1G44180 | 66.047 | 430 | 145 | 1 | 12 | 441 | 7 | 435 | 0.0 | 615 |
| MS.gene053778.t1 | AT4G38220 | 53.171 | 410 | 186 | 2 | 33 | 442 | 21 | 424 | 1.04e-163 | 468 |
| MS.gene053778.t1 | AT4G38220 | 53.285 | 411 | 187 | 3 | 33 | 442 | 21 | 427 | 1.73e-163 | 468 |
Find 138 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCTTCTCTTTACATGGCC+TGG | 0.205801 | 5.1:+45419505 | MS.gene053778:CDS |
| CTTCGGTTGGTGATGAGTTT+AGG | 0.214559 | 5.1:+45419860 | MS.gene053778:CDS |
| AGGACCCATCAGAGACTATT+TGG | 0.216846 | 5.1:+45426403 | MS.gene053778:CDS |
| GTTGTGGATTGAAGAATTTC+AGG | 0.246803 | 5.1:-45426518 | None:intergenic |
| TCATTAGTTGCAGTCATTAA+AGG | 0.282446 | 5.1:-45426431 | None:intergenic |
| TTCGGTTGGTGATGAGTTTA+GGG | 0.303395 | 5.1:+45419861 | MS.gene053778:CDS |
| TCCTTCTCTTTACATGGCCT+GGG | 0.318452 | 5.1:+45419506 | MS.gene053778:CDS |
| GCGGAGGTGTAATCTGGATT+TGG | 0.322659 | 5.1:-45419403 | None:intergenic |
| GGATGGAAGGGAGAGAAGGG+TGG | 0.335116 | 5.1:-45419531 | None:intergenic |
| GATGAGGAAGTTGGTGGATT+TGG | 0.346416 | 5.1:+45419763 | MS.gene053778:CDS |
| ACTCCAGCCTTAACATAAAC+TGG | 0.359930 | 5.1:-45420081 | None:intergenic |
| CAGAGTCGAGATGGGAGTTC+AGG | 0.364976 | 5.1:-45419555 | None:intergenic |
| GTTGAATTCCGAGGTTGTTT+CGG | 0.366528 | 5.1:+45420053 | MS.gene053778:CDS |
| TGGGAGTTCAGGAGGATGGA+AGG | 0.377147 | 5.1:-45419544 | None:intergenic |
| GAAATAGCGGAGGTGTAATC+TGG | 0.382097 | 5.1:-45419409 | None:intergenic |
| GGAAGGGATGGAGTTGGATT+GGG | 0.382893 | 5.1:-45419443 | None:intergenic |
| CAGTTTGATGTTGTTAAGGC+TGG | 0.384823 | 5.1:+45420024 | MS.gene053778:CDS |
| TCCCTGCTGAACCTTCTAAA+TGG | 0.394541 | 5.1:+45419578 | MS.gene053778:CDS |
| GGACCCATCAGAGACTATTT+GGG | 0.396497 | 5.1:+45426404 | MS.gene053778:CDS |
| ATTCTCCTCTTCATTTCGTC+AGG | 0.396666 | 5.1:-45420493 | None:intergenic |
| TGGTGGTTGGCAGTTGTTTG+TGG | 0.402438 | 5.1:+45419783 | MS.gene053778:CDS |
| GGAGGATGGAAGGGAGAGAA+GGG | 0.410132 | 5.1:-45419534 | None:intergenic |
| AGCCTCTCCTTCTCTTTACA+TGG | 0.413508 | 5.1:+45419500 | MS.gene053778:CDS |
| CGGAGGTGTAATCTGGATTT+GGG | 0.414688 | 5.1:-45419402 | None:intergenic |
| GAGTCAGTTTGATGTTGTTA+AGG | 0.415690 | 5.1:+45420020 | MS.gene053778:CDS |
| CAGAAAGGGATTCCTGTACT+TGG | 0.416073 | 5.1:+45426560 | MS.gene053778:CDS |
| AGGAGGATGGAAGGGAGAGA+AGG | 0.421404 | 5.1:-45419535 | None:intergenic |
| GGAGGATGGAGCCATTTAGA+AGG | 0.426494 | 5.1:-45419589 | None:intergenic |
| ATGGCTCCATCCTCCTTTCT+CGG | 0.428377 | 5.1:+45419597 | MS.gene053778:CDS |
| GATTAAAGCGAAAGGGCAAC+CGG | 0.433872 | 5.1:+45419918 | MS.gene053778:CDS |
| AGGTTGTTGTTGTTGAGAGA+AGG | 0.435970 | 5.1:-45419312 | None:intergenic |
| GCTTCCCTGGTGAGAATTCA+AGG | 0.436460 | 5.1:-45419480 | None:intergenic |
| TGCAATACCTCGAAGCAATT+CGG | 0.438262 | 5.1:+45419683 | MS.gene053778:CDS |
| TTGTGGTCATGAAGCAAGAT+AGG | 0.439411 | 5.1:-45426605 | None:intergenic |
| GGTTAATCCAGTTTATGTTA+AGG | 0.441495 | 5.1:+45420074 | MS.gene053778:CDS |
| AAGTTGGTGGATTTGGTGGT+TGG | 0.442213 | 5.1:+45419770 | MS.gene053778:CDS |
| GAGGAAGTTGGTGGATTTGG+TGG | 0.443100 | 5.1:+45419766 | MS.gene053778:CDS |
| GGGAGTTCAGGAGGATGGAA+GGG | 0.446162 | 5.1:-45419543 | None:intergenic |
| TGGAAGGGATGGAGTTGGAT+TGG | 0.447611 | 5.1:-45419444 | None:intergenic |
| GGGAGAGAAGGGTGGGACCC+AGG | 0.448707 | 5.1:-45419523 | None:intergenic |
| CAACTTCCTCATCAGGAACT+AGG | 0.449328 | 5.1:-45419753 | None:intergenic |
| GTTGGATTTGCGTTAGATGA+AGG | 0.463575 | 5.1:+45419832 | MS.gene053778:CDS |
| CAACCTTCGGAGGCAGAAGC+TGG | 0.469578 | 5.1:+45420439 | MS.gene053778:CDS |
| GAATTTGAAAAGATGAATGT+TGG | 0.470089 | 5.1:+45419814 | MS.gene053778:CDS |
| GTTGGATTGGGAGGTGAGGT+AGG | 0.470149 | 5.1:-45419431 | None:intergenic |
| GAGATATGAACCGTCCTAAC+CGG | 0.472738 | 5.1:-45419730 | None:intergenic |
| AATCCAGTTTATGTTAAGGC+TGG | 0.478162 | 5.1:+45420078 | MS.gene053778:CDS |
| GTGCACCACGGGCGAAGATA+AGG | 0.479507 | 5.1:-45419639 | None:intergenic |
| GCTTTAATCTTCAAATGCCA+TGG | 0.489025 | 5.1:-45419904 | None:intergenic |
| TATCTTCGCCCGTGGTGCAC+AGG | 0.489257 | 5.1:+45419642 | MS.gene053778:CDS |
| AATCCACCAACTTCCTCATC+AGG | 0.490507 | 5.1:-45419760 | None:intergenic |
| TAGCGTTGGAAACGACTGAT+TGG | 0.490663 | 5.1:-45419358 | None:intergenic |
| TGTGATGAATGTGCAACCTT+CGG | 0.491674 | 5.1:+45420426 | MS.gene053778:CDS |
| CTAGTTCCTGATGAGGAAGT+TGG | 0.492909 | 5.1:+45419754 | MS.gene053778:CDS |
| AGCCATTTAGAAGGTTCAGC+AGG | 0.495114 | 5.1:-45419580 | None:intergenic |
| CAGCAGGGACAGAGTCGAGA+TGG | 0.495587 | 5.1:-45419564 | None:intergenic |
| AATATATCGAGCATCGGTTG+TGG | 0.496361 | 5.1:-45426534 | None:intergenic |
| TTTATCATCCTGTGCACCAC+GGG | 0.498318 | 5.1:-45419650 | None:intergenic |
| TGTGTACATGAAGGGAATAA+AGG | 0.500676 | 5.1:+45426757 | MS.gene053778:CDS |
| ATGGATGGAAGGGATGGAGT+TGG | 0.501034 | 5.1:-45419449 | None:intergenic |
| GTTAGATGAAGGACAAGCTT+CGG | 0.501465 | 5.1:+45419843 | MS.gene053778:CDS |
| GAAAACTAACCACCATGGAT+TGG | 0.501858 | 5.1:-45426456 | None:intergenic |
| GAGGAGAATTGCTGCTGAAT+GGG | 0.503044 | 5.1:+45420507 | MS.gene053778:CDS |
| GCGTCGCGAACGGCCGAGAA+AGG | 0.508774 | 5.1:-45419610 | None:intergenic |
| GCTCGATATATTCGACAGAA+AGG | 0.510109 | 5.1:+45426545 | MS.gene053778:CDS |
| TCGGCCGTTCGCGACGCGGA+CGG | 0.510855 | 5.1:+45419616 | MS.gene053778:CDS |
| AAAATCCTTGAATTCTCACC+AGG | 0.511241 | 5.1:+45419475 | MS.gene053778:CDS |
| TGTTAGAAATATGTCATATG+AGG | 0.518886 | 5.1:+45420537 | MS.gene053778:CDS |
| TACAATCTCGAACCATGCCC+CGG | 0.520996 | 5.1:-45419937 | None:intergenic |
| GGAGGTGAGGTAGGAAATAG+CGG | 0.521837 | 5.1:-45419422 | None:intergenic |
| AGAGGAGAATTGCTGCTGAA+TGG | 0.522314 | 5.1:+45420506 | MS.gene053778:CDS |
| TAAGATTCCGAATTGCTTCG+AGG | 0.522352 | 5.1:-45419690 | None:intergenic |
| TGTTTATGCGAAGGTAGCGT+TGG | 0.524429 | 5.1:-45419372 | None:intergenic |
| TTTGTGATGGATGGAAGGGA+TGG | 0.525198 | 5.1:-45419455 | None:intergenic |
| AAGCAGGCTATAGCCTCAGC+TGG | 0.528719 | 5.1:+45426479 | MS.gene053778:CDS |
| AAGAGAAGGAGAGGCTTCCC+TGG | 0.528914 | 5.1:-45419493 | None:intergenic |
| GTTCCTGATGAGGAAGTTGG+TGG | 0.535928 | 5.1:+45419757 | MS.gene053778:CDS |
| CTCGATATATTCGACAGAAA+GGG | 0.537271 | 5.1:+45426546 | MS.gene053778:CDS |
| TTTATGCGGATCGGTTTCCA+TGG | 0.541080 | 5.1:+45419887 | MS.gene053778:CDS |
| TGATGTTGTTAAGGCTGGGA+AGG | 0.542641 | 5.1:+45420029 | MS.gene053778:CDS |
| ATTTGAAGATTAAAGCGAAA+GGG | 0.543919 | 5.1:+45419911 | MS.gene053778:CDS |
| ACGCCCCAAATAGTCTCTGA+TGG | 0.548595 | 5.1:-45426408 | None:intergenic |
| GCAGTTGTTTGTGGAGTCTG+AGG | 0.551332 | 5.1:+45419792 | MS.gene053778:CDS |
| ATTAAAGCGAAAGGGCAACC+GGG | 0.555182 | 5.1:+45419919 | MS.gene053778:CDS |
| AACTTCCTCATCAGGAACTA+GGG | 0.558763 | 5.1:-45419752 | None:intergenic |
| AGCAGGGACAGAGTCGAGAT+GGG | 0.559470 | 5.1:-45419563 | None:intergenic |
| GATGGAAGGGAGAGAAGGGT+GGG | 0.559746 | 5.1:-45419530 | None:intergenic |
| ATTGGGGAGAAACCAAGTAC+AGG | 0.562420 | 5.1:-45426572 | None:intergenic |
| ACGAGATACTGTGTACATGA+AGG | 0.562710 | 5.1:+45426748 | MS.gene053778:CDS |
| GAGATGGGAGTTCAGGAGGA+TGG | 0.567152 | 5.1:-45419548 | None:intergenic |
| AGTCGAGATGGGAGTTCAGG+AGG | 0.568950 | 5.1:-45419552 | None:intergenic |
| AAACCAGCTTCTGCCTCCGA+AGG | 0.573740 | 5.1:-45420442 | None:intergenic |
| TCGCGAACGGCCGAGAAAGG+AGG | 0.573764 | 5.1:-45419607 | None:intergenic |
| AAGGCCGTCCGCGTCGCGAA+CGG | 0.576733 | 5.1:-45419620 | None:intergenic |
| GCGAAAGGGCAACCGGGGCA+TGG | 0.576892 | 5.1:+45419925 | MS.gene053778:CDS |
| GGCCATGTAAAGAGAAGGAG+AGG | 0.579297 | 5.1:-45419502 | None:intergenic |
| TGGAGTTGGATTGGGAGGTG+AGG | 0.579366 | 5.1:-45419435 | None:intergenic |
| GATGAAGGACAAGCTTCGGT+TGG | 0.580873 | 5.1:+45419847 | MS.gene053778:CDS |
| CGCCCCAAATAGTCTCTGAT+GGG | 0.582098 | 5.1:-45426407 | None:intergenic |
| CAACTAATGATTCCAATCCA+TGG | 0.582221 | 5.1:+45426444 | MS.gene053778:CDS |
| GAACGGCCGAGAAAGGAGGA+TGG | 0.583617 | 5.1:-45419603 | None:intergenic |
| AGTTTGATGTTGTTAAGGCT+GGG | 0.586632 | 5.1:+45420025 | MS.gene053778:CDS |
| CTGTCGAATATATCGAGCAT+CGG | 0.589926 | 5.1:-45426540 | None:intergenic |
| ATTTCGTCAGGATCTGTCGT+AGG | 0.590363 | 5.1:-45420481 | None:intergenic |
| CAGATCCTGACGAAATGAAG+AGG | 0.592088 | 5.1:+45420488 | MS.gene053778:CDS |
| ATTGTATGATGATAGTGCTA+TGG | 0.595056 | 5.1:+45419954 | MS.gene053778:CDS |
| CGAGATACTGTGTACATGAA+GGG | 0.595091 | 5.1:+45426749 | MS.gene053778:CDS |
| ATATTGCAGATCATAGAGAA+AGG | 0.596427 | 5.1:+45426383 | MS.gene053778:intron |
| ATTCGGAATCTTAACAACAA+GGG | 0.596614 | 5.1:+45419700 | MS.gene053778:CDS |
| TGCCAAACTCACATCATGAG+TGG | 0.600194 | 5.1:-45420106 | None:intergenic |
| GAATTTGATGAAGAGTGTTG+AGG | 0.601037 | 5.1:+45419978 | MS.gene053778:CDS |
| AGCTTCAGTAAACGAACTCA+AGG | 0.603836 | 5.1:-45426801 | None:intergenic |
| ACCCAGGCCATGTAAAGAGA+AGG | 0.604148 | 5.1:-45419507 | None:intergenic |
| AGAGTGTTGAGGTTGTGAGT+AGG | 0.605145 | 5.1:+45419989 | MS.gene053778:CDS |
| TGGATTAACCGAAACAACCT+CGG | 0.607325 | 5.1:-45420061 | None:intergenic |
| CTTGCTTCATGACCACAATG+AGG | 0.609150 | 5.1:+45426610 | MS.gene053778:CDS |
| AATTCGGAATCTTAACAACA+AGG | 0.615006 | 5.1:+45419699 | MS.gene053778:CDS |
| TATGACATATTTCTAACAGA+GGG | 0.617026 | 5.1:-45420532 | None:intergenic |
| GCCAAACTCACATCATGAGT+GGG | 0.619380 | 5.1:-45420105 | None:intergenic |
| TTTCTCGGCCGTTCGCGACG+CGG | 0.619967 | 5.1:+45419612 | MS.gene053778:CDS |
| CATTTGAAGATTAAAGCGAA+AGG | 0.620511 | 5.1:+45419910 | MS.gene053778:CDS |
| GACGGCCTTATCTTCGCCCG+TGG | 0.620875 | 5.1:+45419634 | MS.gene053778:CDS |
| GCCATTTAGAAGGTTCAGCA+GGG | 0.636542 | 5.1:-45419579 | None:intergenic |
| TGGGAAGGCGTTGAATTCCG+AGG | 0.643000 | 5.1:+45420044 | MS.gene053778:CDS |
| TATCTCCCTAGTTCCTGATG+AGG | 0.655059 | 5.1:+45419747 | MS.gene053778:CDS |
| TTAAAGCGAAAGGGCAACCG+GGG | 0.660611 | 5.1:+45419920 | MS.gene053778:CDS |
| ATTTATCATCCTGTGCACCA+CGG | 0.661983 | 5.1:-45419651 | None:intergenic |
| TGCTTGAAAACTAACCACCA+TGG | 0.670201 | 5.1:-45426461 | None:intergenic |
| AGGGATGGAGTTGGATTGGG+AGG | 0.670881 | 5.1:-45419440 | None:intergenic |
| ATATGACATATTTCTAACAG+AGG | 0.671509 | 5.1:-45420533 | None:intergenic |
| GATGAATGTGCAACCTTCGG+AGG | 0.672443 | 5.1:+45420429 | MS.gene053778:CDS |
| GGTGAGGTAGGAAATAGCGG+AGG | 0.683458 | 5.1:-45419419 | None:intergenic |
| GACCCATCAGAGACTATTTG+GGG | 0.690098 | 5.1:+45426405 | MS.gene053778:CDS |
| GGTGTGCGGTGTTTATGCGA+AGG | 0.690804 | 5.1:-45419381 | None:intergenic |
| AAATCCTTGAATTCTCACCA+GGG | 0.697803 | 5.1:+45419476 | MS.gene053778:CDS |
| GTAATCTGGATTTGGGTGTG+CGG | 0.704552 | 5.1:-45419395 | None:intergenic |
| CTAATGATTCCAATCCATGG+TGG | 0.746783 | 5.1:+45426447 | MS.gene053778:CDS |
| AAAGTTGTGTAACCTCATTG+TGG | 0.788736 | 5.1:-45426622 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 45419253 | 45426832 | 45419253 | ID=MS.gene053778 |
| chr5.1 | mRNA | 45419253 | 45426832 | 45419253 | ID=MS.gene053778.t1;Parent=MS.gene053778 |
| chr5.1 | exon | 45419253 | 45420116 | 45419253 | ID=MS.gene053778.t1.exon1;Parent=MS.gene053778.t1 |
| chr5.1 | CDS | 45419253 | 45420116 | 45419253 | ID=cds.MS.gene053778.t1;Parent=MS.gene053778.t1 |
| chr5.1 | exon | 45420421 | 45420558 | 45420421 | ID=MS.gene053778.t1.exon2;Parent=MS.gene053778.t1 |
| chr5.1 | CDS | 45420421 | 45420558 | 45420421 | ID=cds.MS.gene053778.t1;Parent=MS.gene053778.t1 |
| chr5.1 | exon | 45426392 | 45426631 | 45426392 | ID=MS.gene053778.t1.exon3;Parent=MS.gene053778.t1 |
| chr5.1 | CDS | 45426392 | 45426631 | 45426392 | ID=cds.MS.gene053778.t1;Parent=MS.gene053778.t1 |
| chr5.1 | exon | 45426743 | 45426832 | 45426743 | ID=MS.gene053778.t1.exon4;Parent=MS.gene053778.t1 |
| chr5.1 | CDS | 45426743 | 45426832 | 45426743 | ID=cds.MS.gene053778.t1;Parent=MS.gene053778.t1 |
| Gene Sequence |
| Protein sequence |
