Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054457.t1 | XP_013451238.1 | 95.7 | 117 | 3 | 1 | 1 | 115 | 349 | 465 | 3.30E-60 | 240.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054457.t1 | A0A072U6H8 | 95.7 | 117 | 3 | 1 | 1 | 115 | 349 | 465 | 2.4e-60 | 240.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054457 | MS.gene054459 | 0.847907 | 8.59E-60 | -1.69E-46 |
| MS.gene054457 | MS.gene054662 | 0.800813 | 1.21E-48 | -1.69E-46 |
| MS.gene054457 | MS.gene06093 | 0.807597 | 4.68E-50 | -1.69E-46 |
| MS.gene054457 | MS.gene063308 | 0.806223 | 9.13E-50 | -1.69E-46 |
| MS.gene054457 | MS.gene070800 | 0.80097 | 1.12E-48 | -1.69E-46 |
| MS.gene054457 | MS.gene23886 | 0.805655 | 1.20E-49 | -1.69E-46 |
| MS.gene054457 | MS.gene37495 | 0.801142 | 1.04E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054457.t1 | MTR_6g016895 | 95.726 | 117 | 3 | 1 | 1 | 115 | 349 | 465 | 1.27e-77 | 236 |
| MS.gene054457.t1 | MTR_6g016895 | 95.652 | 115 | 4 | 1 | 1 | 115 | 349 | 462 | 7.83e-77 | 234 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054457.t1 | AT1G67180 | 44.444 | 117 | 61 | 1 | 3 | 115 | 255 | 371 | 6.45e-28 | 105 |
| MS.gene054457.t1 | AT1G67180 | 44.444 | 117 | 61 | 1 | 3 | 115 | 255 | 371 | 6.45e-28 | 105 |
| MS.gene054457.t1 | AT1G67180 | 44.444 | 117 | 61 | 1 | 3 | 115 | 337 | 453 | 1.02e-27 | 105 |
| MS.gene054457.t1 | AT1G67180 | 44.444 | 117 | 61 | 1 | 3 | 115 | 337 | 453 | 1.02e-27 | 105 |
Find 0 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAATAAATATAGTTTAATA+TGG | + | 42369:14158-14177 | MS.gene054457:intergenic | 10.0% |
| !! | TATTAAACTATATTTATTGT+CGG | - | 42369:14158-14177 | MS.gene054457:intron | 10.0% |
| !!! | TTTTGTGAATGATAAATATT+TGG | - | 42369:14259-14278 | MS.gene054457:intron | 15.0% |
| !! | CAATTGTTGAATAACATATT+AGG | + | 42369:14383-14402 | MS.gene054457:intergenic | 20.0% |
| ! | AAGAATTCATTCTTATTGCA+TGG | - | 42369:14600-14619 | MS.gene054457:CDS | 25.0% |
| ! | ATTCTTTCATTCTAACATGA+AGG | - | 42369:14287-14306 | MS.gene054457:intron | 25.0% |
| ! | TTTCATGATCTTCATAATAC+TGG | + | 42369:14008-14027 | MS.gene054457:intergenic | 25.0% |
| !! | AATCTTTACTGTACACATTT+TGG | - | 42369:14434-14453 | MS.gene054457:intron | 25.0% |
| !! | TGTCTCTAAAAAAATTTCCT+TGG | + | 42369:14320-14339 | MS.gene054457:intergenic | 25.0% |
| !!! | ACTAGAATTTGCTCATTTTA+CGG | - | 42369:14340-14359 | MS.gene054457:intron | 25.0% |
| AATTGGGATACAAAAATCTG+AGG | + | 42369:14095-14114 | MS.gene054457:intergenic | 30.0% | |
| CTGGAAATTCTTGATCAATT+GGG | + | 42369:14111-14130 | MS.gene054457:intergenic | 30.0% | |
| TCTGGAAATTCTTGATCAAT+TGG | + | 42369:14112-14131 | MS.gene054457:intergenic | 30.0% | |
| ! | TTCACAAAATTAAAAAGCGC+AGG | + | 42369:14248-14267 | MS.gene054457:intergenic | 30.0% |
| !! | CTAGAATTTGCTCATTTTAC+GGG | - | 42369:14341-14360 | MS.gene054457:intron | 30.0% |
| AACATGAAGGATGAAAACCA+AGG | - | 42369:14300-14319 | MS.gene054457:intron | 35.0% | |
| ATTTCCAGACAATAGCTTAG+AGG | - | 42369:14123-14142 | MS.gene054457:CDS | 35.0% | |
| CAATTCTCAAGTAGATCTTC+AGG | + | 42369:14562-14581 | MS.gene054457:intergenic | 35.0% | |
| GTAATCACATGGAATTGTCT+GGG | + | 42369:14068-14087 | MS.gene054457:intergenic | 35.0% | |
| TGTAATCACATGGAATTGTC+TGG | + | 42369:14069-14088 | MS.gene054457:intergenic | 35.0% | |
| !! | TTTACTGTACACATTTTGGC+TGG | - | 42369:14438-14457 | MS.gene054457:intron | 35.0% |
| CATACCTCTAAGCTATTGTC+TGG | + | 42369:14130-14149 | MS.gene054457:intergenic | 40.0% | |
| TCAGGTGCTTGTGTAATTTG+CGG | - | 42369:14526-14545 | MS.gene054457:CDS | 40.0% | |
| TTCAAACTGTCCTTTGTGCA+AGG | - | 42369:13982-14001 | MS.gene054457:CDS | 40.0% | |
| ! | ATTTTGCTTGTACAGTCCTC+AGG | - | 42369:14508-14527 | MS.gene054457:intron | 40.0% |
| ! | TCTGAGGTAGTGTAATCACA+TGG | + | 42369:14079-14098 | MS.gene054457:intergenic | 40.0% |
| !! | ATAATACTGGCCTTGCACAA+AGG | + | 42369:13995-14014 | MS.gene054457:intergenic | 40.0% |
| !! | GGAGTATGCTTTTTGATCAG+TGG | + | 42369:14047-14066 | MS.gene054457:intergenic | 40.0% |
| ATGGACATGCACTTCTTGCA+AGG | - | 42369:14639-14658 | MS.gene054457:CDS | 45.0% | |
| GCAAATTACACAAGCACCTG+AGG | + | 42369:14527-14546 | MS.gene054457:intergenic | 45.0% | |
| ! | CTTGTGTAATTTGCGGTGGT+CGG | - | 42369:14533-14552 | MS.gene054457:CDS | 45.0% |
| ! | TTGCAAGAAGTGCATGTCCA+TGG | + | 42369:14640-14659 | MS.gene054457:intergenic | 45.0% |
| ! | TTGTGTAATTTGCGGTGGTC+GGG | - | 42369:14534-14553 | MS.gene054457:CDS | 45.0% |
| !! | AATATTAAAAAATAATAACA+AGG | + | 42369:14189-14208 | MS.gene054457:intergenic | 5.0% |
| ATGTCCATGGTCGCAATGGA+GGG | + | 42369:14627-14646 | MS.gene054457:intergenic | 50.0% | |
| ! | GGTGCTTGTGTAATTTGCGG+TGG | - | 42369:14529-14548 | MS.gene054457:CDS | 50.0% |
| CATGTCCATGGTCGCAATGG+AGG | + | 42369:14628-14647 | MS.gene054457:intergenic | 55.0% | |
| GTGCATGTCCATGGTCGCAA+TGG | + | 42369:14631-14650 | MS.gene054457:intergenic | 55.0% | |
| TGGACCCTCCATTGCGACCA+TGG | - | 42369:14620-14639 | MS.gene054457:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 42369 | gene | 13971 | 14696 | 13971 | ID=MS.gene054457 |
| 42369 | mRNA | 13971 | 14696 | 13971 | ID=MS.gene054457.t1;Parent=MS.gene054457 |
| 42369 | exon | 14523 | 14696 | 14523 | ID=MS.gene054457.t1.exon1;Parent=MS.gene054457.t1 |
| 42369 | CDS | 14523 | 14696 | 14523 | ID=cds.MS.gene054457.t1;Parent=MS.gene054457.t1 |
| 42369 | exon | 13971 | 14144 | 13971 | ID=MS.gene054457.t1.exon2;Parent=MS.gene054457.t1 |
| 42369 | CDS | 13971 | 14144 | 13971 | ID=cds.MS.gene054457.t1;Parent=MS.gene054457.t1 |
| Gene Sequence |
| Protein sequence |
