Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063308.t1 | XP_013453176.1 | 88.5 | 937 | 100 | 3 | 1 | 931 | 1 | 935 | 0.00E+00 | 1481.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063308.t1 | Q94F88 | 54.3 | 786 | 340 | 10 | 139 | 911 | 48 | 827 | 3.3e-236 | 819.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063308.t1 | A0A072UBK7 | 88.5 | 937 | 100 | 3 | 1 | 931 | 1 | 935 | 0.0e+00 | 1481.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049251 | MS.gene063308 | 0.804544 | 2.05E-49 | -1.69E-46 |
| MS.gene049596 | MS.gene063308 | 0.800919 | 1.15E-48 | -1.69E-46 |
| MS.gene049844 | MS.gene063308 | 0.844836 | 5.91E-59 | -1.69E-46 |
| MS.gene050874 | MS.gene063308 | 0.825606 | 4.28E-54 | -1.69E-46 |
| MS.gene050875 | MS.gene063308 | 0.850903 | 1.26E-60 | -1.69E-46 |
| MS.gene05124 | MS.gene063308 | 0.821034 | 5.00E-53 | -1.69E-46 |
| MS.gene051896 | MS.gene063308 | 0.811664 | 6.25E-51 | -1.69E-46 |
| MS.gene051914 | MS.gene063308 | 0.863712 | 2.05E-64 | -1.69E-46 |
| MS.gene052169 | MS.gene063308 | 0.801766 | 7.71E-49 | -1.69E-46 |
| MS.gene052308 | MS.gene063308 | 0.835952 | 1.24E-56 | -1.69E-46 |
| MS.gene052367 | MS.gene063308 | 0.857627 | 1.43E-62 | -1.69E-46 |
| MS.gene053173 | MS.gene063308 | 0.879989 | 7.94E-70 | -1.69E-46 |
| MS.gene053348 | MS.gene063308 | 0.805544 | 1.27E-49 | -1.69E-46 |
| MS.gene053416 | MS.gene063308 | 0.812113 | 4.99E-51 | -1.69E-46 |
| MS.gene053417 | MS.gene063308 | 0.814997 | 1.16E-51 | -1.69E-46 |
| MS.gene054018 | MS.gene063308 | 0.839142 | 1.89E-57 | -1.69E-46 |
| MS.gene054457 | MS.gene063308 | 0.806223 | 9.13E-50 | -1.69E-46 |
| MS.gene054459 | MS.gene063308 | 0.848614 | 5.48E-60 | -1.69E-46 |
| MS.gene054551 | MS.gene063308 | 0.867681 | 1.14E-65 | -1.69E-46 |
| MS.gene054593 | MS.gene063308 | 0.846085 | 2.71E-59 | -1.69E-46 |
| MS.gene054633 | MS.gene063308 | 0.844918 | 5.61E-59 | -1.69E-46 |
| MS.gene054662 | MS.gene063308 | 0.907449 | 5.01E-81 | -1.69E-46 |
| MS.gene055308 | MS.gene063308 | 0.856177 | 3.84E-62 | -1.69E-46 |
| MS.gene055578 | MS.gene063308 | 0.869189 | 3.73E-66 | -1.69E-46 |
| MS.gene055957 | MS.gene063308 | 0.804125 | 2.51E-49 | -1.69E-46 |
| MS.gene056041 | MS.gene063308 | 0.839471 | 1.55E-57 | -1.69E-46 |
| MS.gene05640 | MS.gene063308 | 0.884663 | 1.58E-71 | -1.69E-46 |
| MS.gene05652 | MS.gene063308 | 0.825182 | 5.39E-54 | -1.69E-46 |
| MS.gene05732 | MS.gene063308 | 0.840584 | 7.96E-58 | -1.69E-46 |
| MS.gene057367 | MS.gene063308 | 0.852236 | 5.27E-61 | -1.69E-46 |
| MS.gene057458 | MS.gene063308 | 0.855232 | 7.24E-62 | -1.69E-46 |
| MS.gene057669 | MS.gene063308 | 0.805876 | 1.08E-49 | -1.69E-46 |
| MS.gene058013 | MS.gene063308 | 0.817005 | 4.13E-52 | -1.69E-46 |
| MS.gene058128 | MS.gene063308 | 0.819137 | 1.36E-52 | -1.69E-46 |
| MS.gene058818 | MS.gene063308 | 0.807882 | 4.07E-50 | -1.69E-46 |
| MS.gene058849 | MS.gene063308 | 0.810715 | 1.00E-50 | -1.69E-46 |
| MS.gene058853 | MS.gene063308 | 0.847368 | 1.21E-59 | -1.69E-46 |
| MS.gene059553 | MS.gene063308 | 0.824957 | 6.09E-54 | -1.69E-46 |
| MS.gene060275 | MS.gene063308 | 0.81694 | 4.27E-52 | -1.69E-46 |
| MS.gene060291 | MS.gene063308 | 0.806943 | 6.43E-50 | -1.69E-46 |
| MS.gene060473 | MS.gene063308 | 0.819422 | 1.17E-52 | -1.69E-46 |
| MS.gene060565 | MS.gene063308 | 0.809499 | 1.84E-50 | -1.69E-46 |
| MS.gene06093 | MS.gene063308 | 0.902061 | 1.42E-78 | -1.69E-46 |
| MS.gene061260 | MS.gene063308 | 0.852393 | 4.75E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene063308 | MS.gene98833 | PPI |
| MS.gene80834 | MS.gene063308 | PPI |
| MS.gene041707 | MS.gene063308 | PPI |
| MS.gene42498 | MS.gene063308 | PPI |
| MS.gene063308 | MS.gene40435 | PPI |
| MS.gene05453 | MS.gene063308 | PPI |
| MS.gene063308 | MS.gene05149 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063308.t1 | MTR_6g088715 | 89.904 | 941 | 79 | 5 | 1 | 931 | 1 | 935 | 0.0 | 1713 |
| MS.gene063308.t1 | MTR_5g066780 | 69.958 | 942 | 130 | 9 | 1 | 931 | 1 | 800 | 0.0 | 1239 |
| MS.gene063308.t1 | MTR_6g084890 | 56.170 | 778 | 317 | 9 | 141 | 912 | 60 | 819 | 0.0 | 874 |
| MS.gene063308.t1 | MTR_5g023660 | 56.387 | 775 | 315 | 9 | 141 | 912 | 61 | 815 | 0.0 | 867 |
| MS.gene063308.t1 | MTR_5g023660 | 55.954 | 781 | 314 | 10 | 141 | 912 | 61 | 820 | 0.0 | 863 |
| MS.gene063308.t1 | MTR_4g017850 | 52.375 | 842 | 352 | 11 | 90 | 912 | 16 | 827 | 0.0 | 858 |
| MS.gene063308.t1 | MTR_7g007090 | 55.226 | 775 | 324 | 9 | 141 | 912 | 57 | 811 | 0.0 | 856 |
| MS.gene063308.t1 | MTR_6g037900 | 53.090 | 793 | 326 | 8 | 141 | 912 | 60 | 827 | 0.0 | 825 |
| MS.gene063308.t1 | MTR_8g071100 | 49.808 | 781 | 367 | 13 | 141 | 911 | 194 | 959 | 0.0 | 761 |
| MS.gene063308.t1 | MTR_5g066830 | 84.896 | 192 | 29 | 0 | 740 | 931 | 1 | 192 | 6.73e-115 | 350 |
| MS.gene063308.t1 | MTR_8g062550 | 43.956 | 455 | 137 | 12 | 218 | 651 | 49 | 406 | 6.09e-93 | 300 |
| MS.gene063308.t1 | MTR_2g046020 | 37.260 | 365 | 196 | 9 | 529 | 888 | 1230 | 1566 | 1.70e-63 | 234 |
| MS.gene063308.t1 | MTR_6g065580 | 37.534 | 365 | 195 | 9 | 529 | 888 | 1204 | 1540 | 2.10e-63 | 234 |
| MS.gene063308.t1 | MTR_5g074180 | 34.896 | 192 | 65 | 5 | 206 | 397 | 11 | 142 | 2.75e-22 | 94.4 |
| MS.gene063308.t1 | MTR_4g098770 | 77.333 | 75 | 17 | 0 | 280 | 354 | 34 | 108 | 1.13e-19 | 86.3 |
| MS.gene063308.t1 | MTR_8g442780 | 46.429 | 112 | 32 | 2 | 423 | 534 | 54 | 137 | 4.97e-19 | 86.3 |
| MS.gene063308.t1 | MTR_5g074190 | 39.370 | 127 | 46 | 3 | 275 | 400 | 406 | 502 | 9.76e-17 | 84.7 |
| MS.gene063308.t1 | MTR_2g054840 | 44.828 | 87 | 48 | 0 | 646 | 732 | 15 | 101 | 1.47e-16 | 78.2 |
| MS.gene063308.t1 | MTR_6g088740 | 60.784 | 51 | 20 | 0 | 864 | 914 | 95 | 145 | 3.03e-15 | 74.3 |
| MS.gene063308.t1 | MTR_0331s0010 | 51.562 | 64 | 31 | 0 | 667 | 730 | 33 | 96 | 4.29e-12 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063308.t1 | AT1G69770 | 54.945 | 819 | 345 | 14 | 106 | 911 | 20 | 827 | 0.0 | 856 |
| MS.gene063308.t1 | AT4G19020 | 48.568 | 838 | 392 | 14 | 81 | 911 | 428 | 1233 | 0.0 | 791 |
| MS.gene063308.t1 | AT4G19020 | 48.568 | 838 | 392 | 14 | 81 | 911 | 476 | 1281 | 0.0 | 791 |
| MS.gene063308.t1 | AT1G80740 | 49.491 | 786 | 361 | 11 | 141 | 912 | 19 | 782 | 0.0 | 736 |
| MS.gene063308.t1 | AT5G49160 | 38.606 | 373 | 184 | 12 | 527 | 888 | 1091 | 1429 | 2.43e-61 | 228 |
| MS.gene063308.t1 | AT5G49160 | 38.606 | 373 | 184 | 12 | 527 | 888 | 1179 | 1517 | 3.17e-61 | 228 |
| MS.gene063308.t1 | AT4G14140 | 36.240 | 367 | 201 | 7 | 527 | 888 | 1164 | 1502 | 1.93e-60 | 225 |
| MS.gene063308.t1 | AT4G14140 | 36.240 | 367 | 201 | 7 | 527 | 888 | 1164 | 1502 | 1.93e-60 | 225 |
| MS.gene063308.t1 | AT4G14140 | 36.240 | 367 | 201 | 7 | 527 | 888 | 1190 | 1528 | 2.09e-60 | 225 |
| MS.gene063308.t1 | AT4G08990 | 35.150 | 367 | 205 | 7 | 527 | 888 | 1157 | 1495 | 8.61e-58 | 217 |
| MS.gene063308.t1 | AT4G13610 | 35.616 | 365 | 194 | 9 | 527 | 888 | 1066 | 1392 | 2.47e-57 | 216 |
Find 193 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGCAATTAATGGTCTTTA+TGG | 0.121671 | 6.1:-78154308 | MS.gene063308:CDS |
| TTTCTGGATGGTTGAGTTTA+AGG | 0.146275 | 6.1:+78156628 | None:intergenic |
| TATCCAATTACCTCAGTTTC+TGG | 0.167645 | 6.1:+78156612 | None:intergenic |
| CGCTTAATCTAATAAACTTT+TGG | 0.175666 | 6.1:+78153374 | None:intergenic |
| AATTGGATCTCCGAGAAATT+TGG | 0.220327 | 6.1:+78159876 | None:intergenic |
| TACTCAGAAATTAAAGAAAA+TGG | 0.228338 | 6.1:-78156988 | MS.gene063308:intron |
| TATGCCAGGTACATTCAAAT+TGG | 0.241454 | 6.1:-78151519 | MS.gene063308:intron |
| TAAGCGGCTTCAACCGTTTC+AGG | 0.268356 | 6.1:-78154363 | MS.gene063308:CDS |
| TCATCCACTTCTTCCGCTTC+CGG | 0.293249 | 6.1:-78160048 | MS.gene063308:CDS |
| TTGTCTTGGCCTTGCATCTC+AGG | 0.296396 | 6.1:-78151457 | MS.gene063308:CDS |
| GACACTTGGGAGCCCATTAC+TGG | 0.307373 | 6.1:-78155468 | MS.gene063308:CDS |
| TCTCAGGGTGCTTCTGTTGA+TGG | 0.315920 | 6.1:-78151441 | MS.gene063308:CDS |
| GTTACCGCTGATTCTGTTTC+CGG | 0.316286 | 6.1:-78160240 | MS.gene063308:CDS |
| TGAAAAGAACAAGCAATTAA+TGG | 0.326867 | 6.1:-78154317 | MS.gene063308:CDS |
| TATACCTGATTGTGGGGTTC+TGG | 0.327007 | 6.1:+78152072 | None:intergenic |
| TGTTCGTATGCGGAACAAAA+AGG | 0.332293 | 6.1:-78152657 | MS.gene063308:CDS |
| ATAAACATATACCTTCCTTT+TGG | 0.334429 | 6.1:+78152940 | None:intergenic |
| GGTAACGGCGTTACCGGATA+TGG | 0.342094 | 6.1:+78160257 | None:intergenic |
| ATTCTAGCTGCTGGTGCCTA+TGG | 0.347090 | 6.1:-78154151 | MS.gene063308:CDS |
| TTCTGTACCTGTGGAGTTTG+AGG | 0.349718 | 6.1:-78153745 | MS.gene063308:intron |
| TTGTGACAAACTAGGTTTCT+TGG | 0.354186 | 6.1:-78155512 | MS.gene063308:intron |
| TAACTGCAAGGAAAGGATTA+AGG | 0.363628 | 6.1:-78154613 | MS.gene063308:CDS |
| CACAAAAGAAATAGCATAGT+TGG | 0.374643 | 6.1:+78152280 | None:intergenic |
| CGCGAAAATGCTAGATTACA+AGG | 0.375043 | 6.1:-78151890 | MS.gene063308:CDS |
| CGTTATGATGATAAGGAAAC+TGG | 0.377370 | 6.1:-78155656 | MS.gene063308:CDS |
| GCGTTGGCCGAAACGGTATC+AGG | 0.380355 | 6.1:-78159833 | MS.gene063308:CDS |
| CAGGAACTATGCTAATCCTT+TGG | 0.384045 | 6.1:-78154344 | MS.gene063308:CDS |
| TTGTGGCGGACCTCCTTGTC+AGG | 0.385472 | 6.1:-78154389 | MS.gene063308:CDS |
| CTAGATTTGTACTCTGGTTG+TGG | 0.385732 | 6.1:-78156847 | MS.gene063308:CDS |
| GTCTCTGCTGATTCAACATC+TGG | 0.385813 | 6.1:-78160012 | MS.gene063308:CDS |
| GCCGCTGCCGATTCAGCTAC+TGG | 0.387797 | 6.1:-78160111 | MS.gene063308:CDS |
| GTCAAGCATCTTACCATTGC+CGG | 0.391508 | 6.1:-78154568 | MS.gene063308:intron |
| ATCTCCGAGAAATTTGGAAT+TGG | 0.392896 | 6.1:+78159882 | None:intergenic |
| ATCAGCGGTAACGGCGTTAC+CGG | 0.394737 | 6.1:+78160251 | None:intergenic |
| ACCTTCTCCTGATACCGTTT+CGG | 0.396188 | 6.1:+78159826 | None:intergenic |
| AGTCGTGATGACATGGAATA+TGG | 0.400598 | 6.1:-78153418 | MS.gene063308:CDS |
| GTTACCGCCGATTCAACTTC+CGG | 0.404821 | 6.1:-78160204 | MS.gene063308:CDS |
| TGGATATTGTTCAATACCTT+CGG | 0.408157 | 6.1:-78154288 | MS.gene063308:CDS |
| TTTATTAGATTAAGCGAAAG+TGG | 0.408399 | 6.1:-78153367 | MS.gene063308:intron |
| TTAGCCTCCTCTATCGGAAT+TGG | 0.409510 | 6.1:+78159859 | None:intergenic |
| AAAATGTAGTTGATCTTCTC+AGG | 0.410171 | 6.1:-78154246 | MS.gene063308:CDS |
| CTCAGGGTGCTTCTGTTGAT+GGG | 0.422570 | 6.1:-78151440 | MS.gene063308:CDS |
| AATCCAATGGATTGTCATCC+TGG | 0.423498 | 6.1:+78157818 | None:intergenic |
| TTCAGATCCGTCTTCTTCTT+AGG | 0.425978 | 6.1:+78159973 | None:intergenic |
| TATCCTAGAGCACGACCAAC+TGG | 0.430266 | 6.1:+78151480 | None:intergenic |
| TTCTAGCTGCTGGTGCCTAT+GGG | 0.431768 | 6.1:-78154150 | MS.gene063308:CDS |
| GCTGACCTCAAACCAGTAAT+GGG | 0.433529 | 6.1:+78155456 | None:intergenic |
| GGAAGATGAAGAGGAGGAAG+AGG | 0.437300 | 6.1:-78155744 | MS.gene063308:CDS |
| GGGAGCCCATTACTGGTTTG+AGG | 0.437959 | 6.1:-78155461 | MS.gene063308:intron |
| GTTCCAGTTGGTCGTGCTCT+AGG | 0.442405 | 6.1:-78151483 | MS.gene063308:CDS |
| GTGAGACTCGTAGTAGATCT+AGG | 0.450528 | 6.1:-78160089 | MS.gene063308:CDS |
| AAAAGTTCACGCTCCACATC+AGG | 0.452211 | 6.1:+78152623 | None:intergenic |
| ATGAATTATCAAAGTCGGTT+GGG | 0.454442 | 6.1:-78154175 | MS.gene063308:CDS |
| CGGAGATCCAATTCCGATAG+AGG | 0.454597 | 6.1:-78159866 | MS.gene063308:CDS |
| GTTTCCAATTCCAAATTTCT+CGG | 0.457331 | 6.1:-78159886 | MS.gene063308:CDS |
| ATGCCAGGTACATTCAAATT+GGG | 0.458977 | 6.1:-78151518 | MS.gene063308:intron |
| AGCTGACCTCAAACCAGTAA+TGG | 0.468162 | 6.1:+78155455 | None:intergenic |
| ATGCTTGTAGGGTTGTTTAG+TGG | 0.469486 | 6.1:-78153634 | MS.gene063308:intron |
| CCTCTCGTTGTTGAAGGAGT+GGG | 0.474584 | 6.1:-78155840 | MS.gene063308:CDS |
| AATGAATTATCAAAGTCGGT+TGG | 0.476288 | 6.1:-78154176 | MS.gene063308:CDS |
| TCCTCTCGTTGTTGAAGGAG+TGG | 0.477495 | 6.1:-78155841 | MS.gene063308:CDS |
| GTTAAGATATACCTGATTGT+GGG | 0.480775 | 6.1:+78152065 | None:intergenic |
| GGTTGTGGAGGAATGTCAAC+AGG | 0.483575 | 6.1:-78156832 | MS.gene063308:CDS |
| AATCTCGTTCAAGATCGAAT+AGG | 0.485068 | 6.1:-78158581 | MS.gene063308:intron |
| ACTCATGATGTTATAGAAAG+AGG | 0.488972 | 6.1:-78153768 | MS.gene063308:CDS |
| TAGACCAGAACCCCACAATC+AGG | 0.490039 | 6.1:-78152076 | MS.gene063308:intron |
| TCCCACCATATCCGAGCAAA+AGG | 0.490769 | 6.1:+78152123 | None:intergenic |
| ATTAACAGTGTCACCGGAGG+AGG | 0.491863 | 6.1:+78160140 | None:intergenic |
| CTAAGCTGCGTTGGCCGAAA+CGG | 0.492035 | 6.1:-78159840 | MS.gene063308:CDS |
| ACATTTGTTAAACTACCATC+AGG | 0.492493 | 6.1:-78157097 | MS.gene063308:intron |
| GAGATGTTTGAAGCAGTTGA+TGG | 0.492805 | 6.1:-78158054 | MS.gene063308:CDS |
| TAGTTTAACAAATGTGGAGT+AGG | 0.493722 | 6.1:+78157105 | None:intergenic |
| AGTCTCACCAGTAGCTGAAT+CGG | 0.495085 | 6.1:+78160104 | None:intergenic |
| TATGCAACATGCAGAGATGT+TGG | 0.496031 | 6.1:-78153059 | MS.gene063308:intron |
| TTTCAGAAGGCTTTCTAGGA+AGG | 0.497252 | 6.1:-78154222 | MS.gene063308:CDS |
| ACAAAGTCATCTTCCTCCTC+CGG | 0.498431 | 6.1:-78160153 | MS.gene063308:CDS |
| ACAAAAGAAATAGCATAGTT+GGG | 0.498692 | 6.1:+78152281 | None:intergenic |
| GCAGCACTGTTCGTAAATCT+AGG | 0.504123 | 6.1:-78160182 | MS.gene063308:CDS |
| AAGTTGCTAGATTTGTACTC+TGG | 0.505648 | 6.1:-78156853 | MS.gene063308:CDS |
| CGCAGCTTAGCCTCCTCTAT+CGG | 0.506729 | 6.1:+78159853 | None:intergenic |
| AGTGCTGCCGGAAGTTGAAT+CGG | 0.507191 | 6.1:+78160197 | None:intergenic |
| TTTCTAGGAAGGTATGCATT+GGG | 0.508340 | 6.1:-78154211 | MS.gene063308:CDS |
| CATTCGAAAATTACCGGCAA+TGG | 0.508465 | 6.1:+78154555 | None:intergenic |
| ATGCAACATGCAGAGATGTT+GGG | 0.516499 | 6.1:-78153058 | MS.gene063308:intron |
| CTTTCTAGGAAGGTATGCAT+TGG | 0.516728 | 6.1:-78154212 | MS.gene063308:CDS |
| CTTGAGCTCTAATCTGAGAA+TGG | 0.516934 | 6.1:+78157777 | None:intergenic |
| TAATGAGGAAGATGAAGAGG+AGG | 0.518697 | 6.1:-78155750 | MS.gene063308:CDS |
| TGTCTTGGCCTTGCATCTCA+GGG | 0.519037 | 6.1:-78151456 | MS.gene063308:CDS |
| GATAGCACTCTATCTTGCTC+TGG | 0.520203 | 6.1:+78151917 | None:intergenic |
| GATTAGCATAGTTCCTGAAA+CGG | 0.521337 | 6.1:+78154350 | None:intergenic |
| TTAAAATGAATTATCAAAGT+CGG | 0.522583 | 6.1:-78154180 | MS.gene063308:CDS |
| TGATGTACGTTATGATGATA+AGG | 0.525372 | 6.1:-78155663 | MS.gene063308:CDS |
| CAATTACCTCAGTTTCTGGA+TGG | 0.527764 | 6.1:+78156616 | None:intergenic |
| CGTGCTCTAGGATATTGTCT+TGG | 0.528052 | 6.1:-78151471 | MS.gene063308:CDS |
| GGCAGTATGTCCTTCGCTAT+AGG | 0.530310 | 6.1:+78153611 | None:intergenic |
| CGGTTGGGAATTCTAGCTGC+TGG | 0.531267 | 6.1:-78154160 | MS.gene063308:CDS |
| GACAAACTAGGTTTCTTGGA+AGG | 0.531491 | 6.1:-78155508 | MS.gene063308:intron |
| ATGGGGAGCCGCACCTTCAG+AGG | 0.535321 | 6.1:-78154098 | MS.gene063308:intron |
| TGGCTGGGAAGCGTGAATCT+AGG | 0.536174 | 6.1:-78160290 | MS.gene063308:CDS |
| GGATCTGAATGCTGGTGTAA+AGG | 0.536302 | 6.1:-78159959 | MS.gene063308:CDS |
| AGATTTACGAACAGTGCTGC+CGG | 0.536628 | 6.1:+78160185 | None:intergenic |
| AGTTTAACAAATGTGGAGTA+GGG | 0.540220 | 6.1:+78157106 | None:intergenic |
| AAGACCGATGATCATGAAGC+AGG | 0.541642 | 6.1:+78153005 | None:intergenic |
| TAGGAAGGTATGCATTGGGC+CGG | 0.544757 | 6.1:-78154207 | MS.gene063308:CDS |
| ATAACATCATGAGTAGGAAG+TGG | 0.545114 | 6.1:+78153777 | None:intergenic |
| AACGCAGTTGCTGTTCCAGT+TGG | 0.547289 | 6.1:-78151495 | MS.gene063308:CDS |
| GGACATGTGGATGTAGTTTG+TGG | 0.548140 | 6.1:-78154406 | MS.gene063308:CDS |
| GGAAACAGAATCAGCGGTAA+CGG | 0.548453 | 6.1:+78160242 | None:intergenic |
| AATCCAGGATGACAATCCAT+TGG | 0.551080 | 6.1:-78157821 | MS.gene063308:CDS |
| TATGCGGATCTGTCAAAGAA+AGG | 0.551479 | 6.1:-78151847 | MS.gene063308:intron |
| AGTTAAGATATACCTGATTG+TGG | 0.553141 | 6.1:+78152064 | None:intergenic |
| GATGATAAGGAAACTGGAGC+TGG | 0.553364 | 6.1:-78155650 | MS.gene063308:CDS |
| GTCAACAGGATTATGTCAAG+GGG | 0.554921 | 6.1:-78156818 | MS.gene063308:CDS |
| ATTCACAGCATCACCGGAAG+CGG | 0.556186 | 6.1:+78160035 | None:intergenic |
| ATTTACCGTGTGTTCGTATG+CGG | 0.556301 | 6.1:-78152667 | MS.gene063308:CDS |
| GAAGAGGAGGAAGAGGATGA+TGG | 0.557865 | 6.1:-78155737 | MS.gene063308:CDS |
| TAGAGGAGGCTAAGCTGCGT+TGG | 0.559672 | 6.1:-78159849 | MS.gene063308:CDS |
| ACCAGTAGCTGAATCGGCAG+CGG | 0.560197 | 6.1:+78160110 | None:intergenic |
| CATTCTCAGATTAGAGCTCA+AGG | 0.564124 | 6.1:-78157776 | MS.gene063308:intron |
| TGATGGTAGTTTAACAAATG+TGG | 0.564819 | 6.1:+78157099 | None:intergenic |
| AAGAAGACGGATCTGAATGC+TGG | 0.566247 | 6.1:-78159967 | MS.gene063308:CDS |
| ACAAACTAGGTTTCTTGGAA+GGG | 0.566319 | 6.1:-78155507 | MS.gene063308:intron |
| AGCAACATTAACAGTGTCAC+CGG | 0.569757 | 6.1:+78160134 | None:intergenic |
| GTGCATGTCGAATCCCCAAA+AGG | 0.570792 | 6.1:-78152955 | MS.gene063308:CDS |
| AATAGTAGGTATACCTCTGA+AGG | 0.571890 | 6.1:+78154085 | None:intergenic |
| GGTAACAGAAACAGCATCGC+CGG | 0.573863 | 6.1:+78160221 | None:intergenic |
| AAGACGAAGTTCTTCTAGCT+AGG | 0.575054 | 6.1:-78158440 | MS.gene063308:CDS |
| AGTCCCTGCTTCATGATCAT+CGG | 0.580310 | 6.1:-78153009 | MS.gene063308:CDS |
| TGTGGCGGACCTCCTTGTCA+GGG | 0.586532 | 6.1:-78154388 | MS.gene063308:CDS |
| AAGACACGAAGGCGAAATTG+AGG | 0.587215 | 6.1:+78154124 | None:intergenic |
| TTTGCCTTCAGGAAAACCAC+TGG | 0.588625 | 6.1:-78152603 | MS.gene063308:intron |
| GCATCAGTAACTGCAAGGAA+AGG | 0.590910 | 6.1:-78154620 | MS.gene063308:intron |
| GCCGAAACGGTATCAGGAGA+AGG | 0.593567 | 6.1:-78159827 | MS.gene063308:intron |
| ACAATTCTGATAAAGACACT+TGG | 0.593863 | 6.1:-78155482 | MS.gene063308:CDS |
| TGATGAAAGTCGTGATGACA+TGG | 0.595321 | 6.1:-78153425 | MS.gene063308:CDS |
| TCACCGCCGTCAACATTCAT+CGG | 0.595913 | 6.1:+78159916 | None:intergenic |
| AGATTTCTGTATTCCTCATG+TGG | 0.596033 | 6.1:+78155776 | None:intergenic |
| AGAGACATTCACAGCATCAC+CGG | 0.596233 | 6.1:+78160029 | None:intergenic |
| GATTTGTACTCTGGTTGTGG+AGG | 0.597555 | 6.1:-78156844 | MS.gene063308:CDS |
| TGTCAACAGGATTATGTCAA+GGG | 0.597856 | 6.1:-78156819 | MS.gene063308:CDS |
| TACAGAGGCACAAGTTGATG+AGG | 0.598512 | 6.1:-78158409 | MS.gene063308:CDS |
| TGTTATCATGTAGGGACATG+TGG | 0.599079 | 6.1:-78154419 | MS.gene063308:intron |
| TTCTTAACAAGACAATCCAA+TGG | 0.599271 | 6.1:+78157805 | None:intergenic |
| GCGAAATTGAGGAAGCCCAT+AGG | 0.600633 | 6.1:+78154135 | None:intergenic |
| TCGTAGTAGATCTAGGAAAG+TGG | 0.601711 | 6.1:-78160082 | MS.gene063308:CDS |
| TGTTTAGTGGCCTATAGCGA+AGG | 0.602249 | 6.1:-78153621 | MS.gene063308:CDS |
| ACACATACCTCAAACTCCAC+AGG | 0.602368 | 6.1:+78153738 | None:intergenic |
| TTTCAGGTCATCAAACAACT+AGG | 0.603040 | 6.1:-78157880 | MS.gene063308:intron |
| ATGTCAACAGGATTATGTCA+AGG | 0.605261 | 6.1:-78156820 | MS.gene063308:CDS |
| CAGAAGCACCCTGAGATGCA+AGG | 0.608985 | 6.1:+78151448 | None:intergenic |
| CAAGAAGCCTAAGAAGAAGA+CGG | 0.610921 | 6.1:-78159980 | MS.gene063308:CDS |
| AGAAAGAGGTTCTGTACCTG+TGG | 0.614587 | 6.1:-78153754 | MS.gene063308:CDS |
| ATCACCGGAAGCGGAAGAAG+TGG | 0.616411 | 6.1:+78160044 | None:intergenic |
| AAATCTAGCAACTTCATCTG+TGG | 0.618134 | 6.1:+78156862 | None:intergenic |
| TCTGGTCTAGTGACAACAGT+AGG | 0.618650 | 6.1:+78152090 | None:intergenic |
| ATGTCGAATCCCCAAAAGGA+AGG | 0.619248 | 6.1:-78152951 | MS.gene063308:intron |
| CCCACTCCTTCAACAACGAG+AGG | 0.622287 | 6.1:+78155840 | None:intergenic |
| AGACCGATGATCATGAAGCA+GGG | 0.623183 | 6.1:+78153006 | None:intergenic |
| TTAAGATATACCTGATTGTG+GGG | 0.623431 | 6.1:+78152066 | None:intergenic |
| TGAGTAGGAAGTGGAAATTG+AGG | 0.626150 | 6.1:+78153786 | None:intergenic |
| ATTAAGGACTTTGTCACTCG+CGG | 0.627454 | 6.1:-78154597 | MS.gene063308:CDS |
| CCTATGCATCAGTAACTGCA+AGG | 0.629663 | 6.1:-78154625 | MS.gene063308:intron |
| TAGGTATACCTCTGAAGGTG+CGG | 0.630743 | 6.1:+78154090 | None:intergenic |
| CAATTCTGATAAAGACACTT+GGG | 0.632620 | 6.1:-78155481 | MS.gene063308:CDS |
| GGTCGAATTGAATCCTGATG+TGG | 0.634295 | 6.1:-78152636 | MS.gene063308:CDS |
| CCTCCTTGTCAGGGAGTAAG+CGG | 0.635996 | 6.1:-78154379 | MS.gene063308:CDS |
| CTTTCTATAACATCATGAGT+AGG | 0.640155 | 6.1:+78153771 | None:intergenic |
| TTGTTCCGCATACGAACACA+CGG | 0.643671 | 6.1:+78152662 | None:intergenic |
| TTATGATGATGCGTATGTGC+AGG | 0.645095 | 6.1:-78158370 | MS.gene063308:intron |
| TTAATGAAAATCTAGGCCGA+AGG | 0.647575 | 6.1:+78154272 | None:intergenic |
| TCTGAATGCTGGTGTAAAGG+AGG | 0.649821 | 6.1:-78159956 | MS.gene063308:CDS |
| ACAGAGGCACAAGTTGATGA+GGG | 0.649951 | 6.1:-78158408 | MS.gene063308:CDS |
| AAAAGAACAAGCTTCTACTG+CGG | 0.653435 | 6.1:-78151382 | MS.gene063308:CDS |
| GTATCGCAGTAATAATCGCA+AGG | 0.655981 | 6.1:+78157142 | None:intergenic |
| TGATCAGGTAGCGTGTACAA+AGG | 0.658061 | 6.1:+78151417 | None:intergenic |
| ACTGTTCCGATGAATGTTGA+CGG | 0.661075 | 6.1:-78159922 | MS.gene063308:CDS |
| AACATTAACAGTGTCACCGG+AGG | 0.670529 | 6.1:+78160137 | None:intergenic |
| ACTCAACCATCCAGAAACTG+AGG | 0.671083 | 6.1:-78156622 | MS.gene063308:intron |
| AGATCCAATTCCGATAGAGG+AGG | 0.671781 | 6.1:-78159863 | MS.gene063308:CDS |
| ATCGCCGGAAACAGAATCAG+CGG | 0.674669 | 6.1:+78160236 | None:intergenic |
| ATGTTTGAAGCAGTTGATGG+AGG | 0.674704 | 6.1:-78158051 | MS.gene063308:CDS |
| CCGCTTACTCCCTGACAAGG+AGG | 0.675819 | 6.1:+78154379 | None:intergenic |
| CAACAGGATTATGTCAAGGG+GGG | 0.682738 | 6.1:-78156816 | MS.gene063308:CDS |
| TGAAGTGAATGCAAAATCTG+AGG | 0.683128 | 6.1:-78156932 | MS.gene063308:CDS |
| AAGCCGCTTACTCCCTGACA+AGG | 0.684437 | 6.1:+78154376 | None:intergenic |
| CGTGATGACATGGAATATGG+CGG | 0.689162 | 6.1:-78153415 | MS.gene063308:CDS |
| CATGTGGATGTAGTTTGTGG+CGG | 0.689828 | 6.1:-78154403 | MS.gene063308:CDS |
| GCTGCCGGAAGTTGAATCGG+CGG | 0.702269 | 6.1:+78160200 | None:intergenic |
| TCAACAGGATTATGTCAAGG+GGG | 0.717776 | 6.1:-78156817 | MS.gene063308:CDS |
| AGCTAGGTGTCACTATACAG+AGG | 0.742048 | 6.1:-78158424 | MS.gene063308:CDS |
| TCGCAGTAATAATCGCAAGG+TGG | 0.748769 | 6.1:+78157145 | None:intergenic |
| GACTGTATTTCACAGCACAG+TGG | 0.764329 | 6.1:-78158029 | MS.gene063308:CDS |
| GTTCCGATGAATGTTGACGG+CGG | 0.834763 | 6.1:-78159919 | MS.gene063308:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 78151350 | 78160313 | 78151350 | ID=MS.gene063308 |
| chr6.1 | mRNA | 78151350 | 78160313 | 78151350 | ID=MS.gene063308.t1;Parent=MS.gene063308 |
| chr6.1 | exon | 78159828 | 78160313 | 78159828 | ID=MS.gene063308.t1.exon1;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78159828 | 78160313 | 78159828 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| chr6.1 | exon | 78158582 | 78158613 | 78158582 | ID=MS.gene063308.t1.exon2;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78158582 | 78158613 | 78158582 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| chr6.1 | exon | 78158371 | 78158467 | 78158371 | ID=MS.gene063308.t1.exon3;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78158371 | 78158467 | 78158371 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| chr6.1 | exon | 78158008 | 78158115 | 78158008 | ID=MS.gene063308.t1.exon4;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78158008 | 78158115 | 78158008 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| chr6.1 | exon | 78157777 | 78157896 | 78157777 | ID=MS.gene063308.t1.exon5;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78157777 | 78157896 | 78157777 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| chr6.1 | exon | 78157098 | 78157197 | 78157098 | ID=MS.gene063308.t1.exon6;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78157098 | 78157197 | 78157098 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| chr6.1 | exon | 78156789 | 78157003 | 78156789 | ID=MS.gene063308.t1.exon7;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78156789 | 78157003 | 78156789 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| chr6.1 | exon | 78156623 | 78156688 | 78156623 | ID=MS.gene063308.t1.exon8;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78156623 | 78156688 | 78156623 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| chr6.1 | exon | 78155637 | 78155888 | 78155637 | ID=MS.gene063308.t1.exon9;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78155637 | 78155888 | 78155637 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| chr6.1 | exon | 78155462 | 78155520 | 78155462 | ID=MS.gene063308.t1.exon10;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78155462 | 78155520 | 78155462 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| chr6.1 | exon | 78154569 | 78154635 | 78154569 | ID=MS.gene063308.t1.exon11;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78154569 | 78154635 | 78154569 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| chr6.1 | exon | 78154099 | 78154428 | 78154099 | ID=MS.gene063308.t1.exon12;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78154099 | 78154428 | 78154099 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| chr6.1 | exon | 78153746 | 78153814 | 78153746 | ID=MS.gene063308.t1.exon13;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78153746 | 78153814 | 78153746 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| chr6.1 | exon | 78153560 | 78153646 | 78153560 | ID=MS.gene063308.t1.exon14;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78153560 | 78153646 | 78153560 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| chr6.1 | exon | 78153368 | 78153455 | 78153368 | ID=MS.gene063308.t1.exon15;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78153368 | 78153455 | 78153368 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| chr6.1 | exon | 78152952 | 78153067 | 78152952 | ID=MS.gene063308.t1.exon16;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78152952 | 78153067 | 78152952 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| chr6.1 | exon | 78152604 | 78152705 | 78152604 | ID=MS.gene063308.t1.exon17;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78152604 | 78152705 | 78152604 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| chr6.1 | exon | 78152263 | 78152306 | 78152263 | ID=MS.gene063308.t1.exon18;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78152263 | 78152306 | 78152263 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| chr6.1 | exon | 78152077 | 78152146 | 78152077 | ID=MS.gene063308.t1.exon19;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78152077 | 78152146 | 78152077 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| chr6.1 | exon | 78151848 | 78151951 | 78151848 | ID=MS.gene063308.t1.exon20;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78151848 | 78151951 | 78151848 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| chr6.1 | exon | 78151350 | 78151533 | 78151350 | ID=MS.gene063308.t1.exon21;Parent=MS.gene063308.t1 |
| chr6.1 | CDS | 78151350 | 78151533 | 78151350 | ID=cds.MS.gene063308.t1;Parent=MS.gene063308.t1 |
| Gene Sequence |
| Protein sequence |
