Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054564.t1 | XP_013454572.1 | 84.4 | 250 | 37 | 1 | 1 | 248 | 1 | 250 | 2.60E-86 | 328.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054564.t1 | Q9LJB7 | 36.5 | 233 | 118 | 3 | 8 | 239 | 5 | 208 | 2.2e-17 | 90.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054564.t1 | A0A072UHJ8 | 84.4 | 250 | 37 | 1 | 1 | 248 | 1 | 250 | 1.9e-86 | 328.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053162 | MS.gene054564 | 0.8698 | 2.36E-66 | -1.69E-46 |
| MS.gene053505 | MS.gene054564 | 0.804081 | 2.56E-49 | -1.69E-46 |
| MS.gene054564 | MS.gene071925 | 0.811561 | 6.58E-51 | -1.69E-46 |
| MS.gene054564 | MS.gene21345 | 0.809782 | 1.60E-50 | -1.69E-46 |
| MS.gene054564 | MS.gene38308 | 0.922244 | 1.26E-88 | -1.69E-46 |
| MS.gene054564 | MS.gene91597 | 0.801863 | 7.37E-49 | -1.69E-46 |
| MS.gene054564 | MS.gene98727 | 0.826237 | 3.03E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054564.t1 | MTR_4g008050 | 90.800 | 250 | 21 | 1 | 1 | 248 | 1 | 250 | 5.23e-154 | 428 |
| MS.gene054564.t1 | MTR_4g046640 | 48.485 | 66 | 34 | 0 | 8 | 73 | 36 | 101 | 2.49e-14 | 68.2 |
| MS.gene054564.t1 | MTR_2g089310 | 51.515 | 66 | 32 | 0 | 6 | 71 | 3 | 68 | 7.07e-14 | 69.3 |
| MS.gene054564.t1 | MTR_2g011450 | 50.000 | 58 | 29 | 0 | 6 | 63 | 3 | 60 | 1.31e-13 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054564.t1 | AT3G21150 | 30.769 | 234 | 132 | 3 | 8 | 240 | 5 | 209 | 3.23e-25 | 99.8 |
| MS.gene054564.t1 | AT4G27310 | 48.750 | 80 | 40 | 1 | 6 | 85 | 3 | 81 | 4.05e-17 | 77.8 |
| MS.gene054564.t1 | AT5G54470 | 53.333 | 75 | 34 | 1 | 3 | 77 | 1 | 74 | 9.58e-17 | 76.6 |
| MS.gene054564.t1 | AT3G21890 | 56.863 | 51 | 22 | 0 | 7 | 57 | 30 | 80 | 1.33e-13 | 65.9 |
| MS.gene054564.t1 | AT4G15248 | 42.857 | 77 | 44 | 0 | 8 | 84 | 32 | 108 | 1.81e-13 | 65.5 |
Find 63 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGAGGTTGGAGAAAATATC+AGG | 0.281886 | 4.1:+87494156 | MS.gene054564:CDS |
| CCGATTCCGCCTTTCTCTGC+TGG | 0.288877 | 4.1:+87493587 | MS.gene054564:CDS |
| ATCGGTGAAACCGTCGCATT+TGG | 0.305273 | 4.1:-87493677 | None:intergenic |
| CGCACCCTTCTTGCAATTCA+AGG | 0.341656 | 4.1:-87494239 | None:intergenic |
| GCGAAATGGAGAAGAGAGTT+AGG | 0.346518 | 4.1:+87493973 | MS.gene054564:CDS |
| TTCTTATTGTTCTCCGGTGC+CGG | 0.351468 | 4.1:-87493901 | None:intergenic |
| AAGAAGATGATCGGGGATAA+AGG | 0.373096 | 4.1:+87493922 | MS.gene054564:CDS |
| ATCTTCTTCTTATTGTTCTC+CGG | 0.395014 | 4.1:-87493907 | None:intergenic |
| TGGCTGCTGCGACGTCGTTT+TGG | 0.398824 | 4.1:+87494082 | MS.gene054564:CDS |
| ACAATAAGAAGAAGATGATC+GGG | 0.406679 | 4.1:+87493914 | MS.gene054564:CDS |
| CGAAATGGAGAAGAGAGTTA+GGG | 0.429114 | 4.1:+87493974 | MS.gene054564:CDS |
| CCAGCAGAGAAAGGCGGAAT+CGG | 0.438734 | 4.1:-87493587 | None:intergenic |
| CGGAGGAGATATTTGCGAAA+TGG | 0.441862 | 4.1:+87493959 | MS.gene054564:CDS |
| AGCTGCGACGACGCCGTTCA+CGG | 0.445971 | 4.1:+87493610 | MS.gene054564:CDS |
| TTCCGGTGAGCAAGAGCGGC+AGG | 0.464761 | 4.1:-87493743 | None:intergenic |
| GTCGCAGCAGCCACCTTCAC+CGG | 0.482296 | 4.1:-87494072 | None:intergenic |
| AACAATAAGAAGAAGATGAT+CGG | 0.487833 | 4.1:+87493913 | MS.gene054564:CDS |
| GAGAACGTGGAAGTTACTTC+CGG | 0.494776 | 4.1:+87494053 | MS.gene054564:CDS |
| GTGGCTAAAATAAGCTTAGC+TGG | 0.495492 | 4.1:-87494183 | None:intergenic |
| CGGGGATAAAGGTGTTGAAT+CGG | 0.508934 | 4.1:+87493933 | MS.gene054564:CDS |
| TTCACCGATATTCATATCTC+CGG | 0.518121 | 4.1:+87493691 | MS.gene054564:CDS |
| AGGTGTTGAATCGGTGGCGG+AGG | 0.520405 | 4.1:+87493942 | MS.gene054564:CDS |
| GTGTCGAAATTTGATGAGGT+TGG | 0.526675 | 4.1:+87494143 | MS.gene054564:CDS |
| GCCACACGATCACCGTTCAC+CGG | 0.535889 | 4.1:-87494006 | None:intergenic |
| AACCTGCCGCTCTTGCTCAC+CGG | 0.536391 | 4.1:+87493741 | MS.gene054564:CDS |
| GTCGCAGCTCCAGCAGAGAA+AGG | 0.536817 | 4.1:-87493596 | None:intergenic |
| CGATGATACATCTCCGGCAC+CGG | 0.539008 | 4.1:+87493888 | MS.gene054564:CDS |
| GAACGGTGATCGTGTGGCGG+TGG | 0.541705 | 4.1:+87494011 | MS.gene054564:CDS |
| TGAGGTTGGAGAAAATATCA+GGG | 0.543347 | 4.1:+87494157 | MS.gene054564:CDS |
| TCGTGTGGCGGTGGAAGCTA+TGG | 0.543504 | 4.1:+87494020 | MS.gene054564:CDS |
| ACGGTGGTGTAGTGTAGTGC+CGG | 0.554858 | 4.1:-87493710 | None:intergenic |
| CAGAGAAAGGCGGAATCGGA+AGG | 0.556584 | 4.1:-87493583 | None:intergenic |
| AACACCTTGAATTGCAAGAA+GGG | 0.556767 | 4.1:+87494235 | MS.gene054564:CDS |
| TCACCGGAAAATCATTCCTC+CGG | 0.557681 | 4.1:+87493757 | MS.gene054564:CDS |
| AGATTGAGAACTCGCCTCAC+CGG | 0.561454 | 4.1:-87493776 | None:intergenic |
| TCCGGTGAACGGTGATCGTG+TGG | 0.570131 | 4.1:+87494005 | MS.gene054564:CDS |
| CAACACCTTGAATTGCAAGA+AGG | 0.572050 | 4.1:+87494234 | MS.gene054564:CDS |
| GTGGAAGTTACTTCCGGTGA+AGG | 0.577957 | 4.1:+87494059 | MS.gene054564:CDS |
| GGATAAAGGTGTTGAATCGG+TGG | 0.581180 | 4.1:+87493936 | MS.gene054564:CDS |
| TAAAGGTGTTGAATCGGTGG+CGG | 0.581743 | 4.1:+87493939 | MS.gene054564:CDS |
| AGTGCCGGAGATATGAATAT+CGG | 0.586763 | 4.1:-87493695 | None:intergenic |
| GAAGTTACTTCCGGTGAAGG+TGG | 0.596707 | 4.1:+87494062 | MS.gene054564:CDS |
| GAGGAAGTTAGCGCCGTGAA+CGG | 0.601829 | 4.1:-87493623 | None:intergenic |
| ATCTTGTGCTCCAAATGCGA+CGG | 0.602290 | 4.1:+87493667 | MS.gene054564:CDS |
| AGCGGCAGGTTGATGAGAGA+CGG | 0.602586 | 4.1:-87493729 | None:intergenic |
| CGGAGATGTATCATCGGTAA+CGG | 0.606238 | 4.1:-87493881 | None:intergenic |
| CGTTACCGATGATACATCTC+CGG | 0.613031 | 4.1:+87493882 | MS.gene054564:CDS |
| CCACGTGTCGAAATTTGATG+AGG | 0.617602 | 4.1:+87494139 | MS.gene054564:CDS |
| ATTTGGAGCACAAGATGTGA+CGG | 0.631824 | 4.1:-87493660 | None:intergenic |
| CCTCATCAAATTTCGACACG+TGG | 0.641370 | 4.1:-87494139 | None:intergenic |
| TGTGGCGGTGGAAGCTATGG+CGG | 0.643579 | 4.1:+87494023 | MS.gene054564:CDS |
| TTGAGAACTCGCCTCACCGG+AGG | 0.643923 | 4.1:-87493773 | None:intergenic |
| CGGTGCCGGAGATGTATCAT+CGG | 0.645343 | 4.1:-87493887 | None:intergenic |
| GGTGAACGGTGATCGTGTGG+CGG | 0.678941 | 4.1:+87494008 | MS.gene054564:CDS |
| GGAAAATCATTCCTCCGGTG+AGG | 0.685941 | 4.1:+87493762 | MS.gene054564:CDS |
| CAATAAGAAGAAGATGATCG+GGG | 0.688051 | 4.1:+87493915 | MS.gene054564:CDS |
| ACGTGCGAGTTTCACATGTG+TGG | 0.691088 | 4.1:-87494202 | None:intergenic |
| GGCAGGTTGATGAGAGACGG+TGG | 0.691500 | 4.1:-87493726 | None:intergenic |
| TGGCGGTGTGTATGAGAACG+TGG | 0.692845 | 4.1:+87494040 | MS.gene054564:CDS |
| GCAGCTCCAGCAGAGAAAGG+CGG | 0.695059 | 4.1:-87493593 | None:intergenic |
| GAGGTTGGAGAAAATATCAG+GGG | 0.723090 | 4.1:+87494158 | MS.gene054564:CDS |
| TGGAGCACAAGATGTGACGG+AGG | 0.724489 | 4.1:-87493657 | None:intergenic |
| GACGGAGGTGACGAGCGACG+AGG | 0.727759 | 4.1:-87493642 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACAATAAGAAGAAGATGAT+CGG | + | chr4.1:87493913-87493932 | MS.gene054564:CDS | 25.0% |
| ACAATAAGAAGAAGATGATC+GGG | + | chr4.1:87493914-87493933 | MS.gene054564:CDS | 30.0% | |
| ATCTTCTTCTTATTGTTCTC+CGG | - | chr4.1:87493910-87493929 | None:intergenic | 30.0% | |
| AACACCTTGAATTGCAAGAA+GGG | + | chr4.1:87494235-87494254 | MS.gene054564:CDS | 35.0% | |
| ATGAGGTTGGAGAAAATATC+AGG | + | chr4.1:87494156-87494175 | MS.gene054564:CDS | 35.0% | |
| CAATAAGAAGAAGATGATCG+GGG | + | chr4.1:87493915-87493934 | MS.gene054564:CDS | 35.0% | |
| TGAGGTTGGAGAAAATATCA+GGG | + | chr4.1:87494157-87494176 | MS.gene054564:CDS | 35.0% | |
| TTCACCGATATTCATATCTC+CGG | + | chr4.1:87493691-87493710 | MS.gene054564:CDS | 35.0% | |
| ! | AGAGTTAGGGTTAGATTTTC+CGG | + | chr4.1:87493987-87494006 | MS.gene054564:CDS | 35.0% |
| AAGAAGATGATCGGGGATAA+AGG | + | chr4.1:87493922-87493941 | MS.gene054564:CDS | 40.0% | |
| CAACACCTTGAATTGCAAGA+AGG | + | chr4.1:87494234-87494253 | MS.gene054564:CDS | 40.0% | |
| CGAAATGGAGAAGAGAGTTA+GGG | + | chr4.1:87493974-87493993 | MS.gene054564:CDS | 40.0% | |
| GAGGTTGGAGAAAATATCAG+GGG | + | chr4.1:87494158-87494177 | MS.gene054564:CDS | 40.0% | |
| GTGGCTAAAATAAGCTTAGC+TGG | - | chr4.1:87494186-87494205 | None:intergenic | 40.0% | |
| GTGTCGAAATTTGATGAGGT+TGG | + | chr4.1:87494143-87494162 | MS.gene054564:CDS | 40.0% | |
| ! | AGTGCCGGAGATATGAATAT+CGG | - | chr4.1:87493698-87493717 | None:intergenic | 40.0% |
| ! | ATTTGGAGCACAAGATGTGA+CGG | - | chr4.1:87493663-87493682 | None:intergenic | 40.0% |
| ! | GGTTGGGGTTAAGATTTTGT+GGG | + | chr4.1:87494103-87494122 | MS.gene054564:CDS | 40.0% |
| ! | GTTGGGGTTAAGATTTTGTG+GGG | + | chr4.1:87494104-87494123 | MS.gene054564:CDS | 40.0% |
| ! | TGGTTGGGGTTAAGATTTTG+TGG | + | chr4.1:87494102-87494121 | MS.gene054564:CDS | 40.0% |
| ATCTTGTGCTCCAAATGCGA+CGG | + | chr4.1:87493667-87493686 | MS.gene054564:CDS | 45.0% | |
| CCACGTGTCGAAATTTGATG+AGG | + | chr4.1:87494139-87494158 | MS.gene054564:CDS | 45.0% | |
| CCTCATCAAATTTCGACACG+TGG | - | chr4.1:87494142-87494161 | None:intergenic | 45.0% | |
| CGGAGATGTATCATCGGTAA+CGG | - | chr4.1:87493884-87493903 | None:intergenic | 45.0% | |
| CGGAGGAGATATTTGCGAAA+TGG | + | chr4.1:87493959-87493978 | MS.gene054564:CDS | 45.0% | |
| CGTTACCGATGATACATCTC+CGG | + | chr4.1:87493882-87493901 | MS.gene054564:CDS | 45.0% | |
| GAGAACGTGGAAGTTACTTC+CGG | + | chr4.1:87494053-87494072 | MS.gene054564:CDS | 45.0% | |
| GCGAAATGGAGAAGAGAGTT+AGG | + | chr4.1:87493973-87493992 | MS.gene054564:CDS | 45.0% | |
| TCACCGGAAAATCATTCCTC+CGG | + | chr4.1:87493757-87493776 | MS.gene054564:CDS | 45.0% | |
| TTCTTATTGTTCTCCGGTGC+CGG | - | chr4.1:87493904-87493923 | None:intergenic | 45.0% | |
| ! | GGGTTAGATTTTCCGGTGAA+CGG | + | chr4.1:87493994-87494013 | MS.gene054564:CDS | 45.0% |
| ! | TCACCGGAGGAATGATTTTC+CGG | - | chr4.1:87493763-87493782 | None:intergenic | 45.0% |
| !! | CGGGGATAAAGGTGTTGAAT+CGG | + | chr4.1:87493933-87493952 | MS.gene054564:CDS | 45.0% |
| !! | GGATAAAGGTGTTGAATCGG+TGG | + | chr4.1:87493936-87493955 | MS.gene054564:CDS | 45.0% |
| !! | TAAAGGTGTTGAATCGGTGG+CGG | + | chr4.1:87493939-87493958 | MS.gene054564:CDS | 45.0% |
| ACGTGCGAGTTTCACATGTG+TGG | - | chr4.1:87494205-87494224 | None:intergenic | 50.0% | |
| AGATTGAGAACTCGCCTCAC+CGG | - | chr4.1:87493779-87493798 | None:intergenic | 50.0% | |
| ATCGGTGAAACCGTCGCATT+TGG | - | chr4.1:87493680-87493699 | None:intergenic | 50.0% | |
| CGCACCCTTCTTGCAATTCA+AGG | - | chr4.1:87494242-87494261 | None:intergenic | 50.0% | |
| GAAGTTACTTCCGGTGAAGG+TGG | + | chr4.1:87494062-87494081 | MS.gene054564:CDS | 50.0% | |
| GGAAAATCATTCCTCCGGTG+AGG | + | chr4.1:87493762-87493781 | MS.gene054564:CDS | 50.0% | |
| GTGGAAGTTACTTCCGGTGA+AGG | + | chr4.1:87494059-87494078 | MS.gene054564:CDS | 50.0% | |
| ! | GATTTTCCGGTGAGCAAGAG+CGG | - | chr4.1:87493750-87493769 | None:intergenic | 50.0% |
| ACGGTGGTGTAGTGTAGTGC+CGG | - | chr4.1:87493713-87493732 | None:intergenic | 55.0% | |
| CAGAGAAAGGCGGAATCGGA+AGG | - | chr4.1:87493586-87493605 | None:intergenic | 55.0% | |
| CCAGCAGAGAAAGGCGGAAT+CGG | - | chr4.1:87493590-87493609 | None:intergenic | 55.0% | |
| CGATGATACATCTCCGGCAC+CGG | + | chr4.1:87493888-87493907 | MS.gene054564:CDS | 55.0% | |
| GAGGAAGTTAGCGCCGTGAA+CGG | - | chr4.1:87493626-87493645 | None:intergenic | 55.0% | |
| TGGAGCACAAGATGTGACGG+AGG | - | chr4.1:87493660-87493679 | None:intergenic | 55.0% | |
| TGGCGGTGTGTATGAGAACG+TGG | + | chr4.1:87494040-87494059 | MS.gene054564:CDS | 55.0% | |
| ! | CGGTGCCGGAGATGTATCAT+CGG | - | chr4.1:87493890-87493909 | None:intergenic | 55.0% |
| !! | AGCGGCAGGTTGATGAGAGA+CGG | - | chr4.1:87493732-87493751 | None:intergenic | 55.0% |
| !! | TGCTGCGACGTCGTTTTGGT+TGG | + | chr4.1:87494086-87494105 | MS.gene054564:CDS | 55.0% |
| !!! | CTGCGACGTCGTTTTGGTTG+GGG | + | chr4.1:87494088-87494107 | MS.gene054564:CDS | 55.0% |
| !!! | GCTGCGACGTCGTTTTGGTT+GGG | + | chr4.1:87494087-87494106 | MS.gene054564:CDS | 55.0% |
| AACCTGCCGCTCTTGCTCAC+CGG | + | chr4.1:87493741-87493760 | MS.gene054564:CDS | 60.0% | |
| CCGATTCCGCCTTTCTCTGC+TGG | + | chr4.1:87493587-87493606 | MS.gene054564:CDS | 60.0% | |
| GCAGCTCCAGCAGAGAAAGG+CGG | - | chr4.1:87493596-87493615 | None:intergenic | 60.0% | |
| GCCACACGATCACCGTTCAC+CGG | - | chr4.1:87494009-87494028 | None:intergenic | 60.0% | |
| GGTGAACGGTGATCGTGTGG+CGG | + | chr4.1:87494008-87494027 | MS.gene054564:CDS | 60.0% | |
| GTCGCAGCTCCAGCAGAGAA+AGG | - | chr4.1:87493599-87493618 | None:intergenic | 60.0% | |
| TCCGGTGAACGGTGATCGTG+TGG | + | chr4.1:87494005-87494024 | MS.gene054564:CDS | 60.0% | |
| TCGTGTGGCGGTGGAAGCTA+TGG | + | chr4.1:87494020-87494039 | MS.gene054564:CDS | 60.0% | |
| TGTGGCGGTGGAAGCTATGG+CGG | + | chr4.1:87494023-87494042 | MS.gene054564:CDS | 60.0% | |
| TTGAGAACTCGCCTCACCGG+AGG | - | chr4.1:87493776-87493795 | None:intergenic | 60.0% | |
| ! | TGGCTGCTGCGACGTCGTTT+TGG | + | chr4.1:87494082-87494101 | MS.gene054564:CDS | 60.0% |
| !! | AGGTGTTGAATCGGTGGCGG+AGG | + | chr4.1:87493942-87493961 | MS.gene054564:CDS | 60.0% |
| !! | GGCAGGTTGATGAGAGACGG+TGG | - | chr4.1:87493729-87493748 | None:intergenic | 60.0% |
| AGCTGCGACGACGCCGTTCA+CGG | + | chr4.1:87493610-87493629 | MS.gene054564:CDS | 65.0% | |
| GAACGGTGATCGTGTGGCGG+TGG | + | chr4.1:87494011-87494030 | MS.gene054564:CDS | 65.0% | |
| GTCGCAGCAGCCACCTTCAC+CGG | - | chr4.1:87494075-87494094 | None:intergenic | 65.0% | |
| TTCCGGTGAGCAAGAGCGGC+AGG | - | chr4.1:87493746-87493765 | None:intergenic | 65.0% | |
| GACGGAGGTGACGAGCGACG+AGG | - | chr4.1:87493645-87493664 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 87493526 | 87494272 | 87493526 | ID=MS.gene054564 |
| chr4.1 | mRNA | 87493526 | 87494272 | 87493526 | ID=MS.gene054564.t1;Parent=MS.gene054564 |
| chr4.1 | exon | 87493526 | 87494272 | 87493526 | ID=MS.gene054564.t1.exon1;Parent=MS.gene054564.t1 |
| chr4.1 | CDS | 87493526 | 87494272 | 87493526 | ID=cds.MS.gene054564.t1;Parent=MS.gene054564.t1 |
| Gene Sequence |
| Protein sequence |
