Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053162.t1 | XP_013454572.1 | 87.2 | 250 | 32 | 0 | 1 | 250 | 1 | 250 | 8.30E-93 | 350.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053162.t1 | Q9LJB7 | 36.6 | 235 | 117 | 3 | 8 | 241 | 5 | 208 | 5.8e-18 | 92.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053162.t1 | A0A072UHJ8 | 87.2 | 250 | 32 | 0 | 1 | 250 | 1 | 250 | 6.0e-93 | 350.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053162 | MS.gene054564 | 0.8698 | 2.36E-66 | -1.69E-46 |
| MS.gene053162 | MS.gene33339 | 0.806066 | 9.85E-50 | -1.69E-46 |
| MS.gene053162 | MS.gene38308 | 0.903134 | 4.75E-79 | -1.69E-46 |
| MS.gene053162 | MS.gene65812 | 0.801301 | 9.60E-49 | -1.69E-46 |
| MS.gene053162 | MS.gene84140 | 0.811999 | 5.28E-51 | -1.69E-46 |
| MS.gene053162 | MS.gene91597 | 0.81182 | 5.78E-51 | -1.69E-46 |
| MS.gene053162 | MS.gene93738 | 0.807222 | 5.62E-50 | -1.69E-46 |
| MS.gene053162 | MS.gene98727 | 0.810328 | 1.22E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053162.t1 | MTR_4g008050 | 92.000 | 250 | 20 | 0 | 1 | 250 | 1 | 250 | 6.98e-161 | 446 |
| MS.gene053162.t1 | MTR_4g046640 | 50.000 | 66 | 33 | 0 | 8 | 73 | 36 | 101 | 1.06e-15 | 72.0 |
| MS.gene053162.t1 | MTR_2g089310 | 50.000 | 66 | 33 | 0 | 6 | 71 | 3 | 68 | 2.68e-12 | 64.7 |
| MS.gene053162.t1 | MTR_2g011450 | 43.939 | 66 | 37 | 0 | 6 | 71 | 3 | 68 | 4.17e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053162.t1 | AT3G21150 | 30.769 | 234 | 132 | 3 | 8 | 241 | 5 | 208 | 9.31e-26 | 101 |
| MS.gene053162.t1 | AT4G27310 | 47.500 | 80 | 41 | 1 | 6 | 85 | 3 | 81 | 2.10e-15 | 73.2 |
| MS.gene053162.t1 | AT3G21890 | 54.386 | 57 | 26 | 0 | 7 | 63 | 30 | 86 | 5.97e-15 | 69.7 |
| MS.gene053162.t1 | AT5G54470 | 52.000 | 75 | 35 | 1 | 3 | 77 | 1 | 74 | 6.66e-15 | 71.6 |
| MS.gene053162.t1 | AT4G15248 | 44.156 | 77 | 43 | 0 | 8 | 84 | 32 | 108 | 1.48e-14 | 68.6 |
Find 61 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGAGGTTGGAGAAAATATC+AGG | 0.281886 | 4.2:+91944991 | MS.gene053162:CDS |
| TTCGGTGAAACCGTCGCATT+TGG | 0.282536 | 4.2:-91944506 | None:intergenic |
| GGTGCCAGCTAAGCTGATTT+TGG | 0.291167 | 4.2:+91945014 | MS.gene053162:CDS |
| CGCACCCTTCTTGCAATTCA+AGG | 0.341656 | 4.2:-91945074 | None:intergenic |
| CCGATTCCGCCTTTCTCTGC+CGG | 0.365012 | 4.2:+91944416 | MS.gene053162:CDS |
| TTGTTATTGTTCTCCGGTGC+CGG | 0.383054 | 4.2:-91944730 | None:intergenic |
| TGGCTGCTGCGACGTCGTTT+TGG | 0.398824 | 4.2:+91944917 | MS.gene053162:CDS |
| TTCCTCTTGTTATTGTTCTC+CGG | 0.408628 | 4.2:-91944736 | None:intergenic |
| AACAAGAGGAAGAAGATGAT+TGG | 0.414945 | 4.2:+91944748 | MS.gene053162:CDS |
| AGCTGCGACGACGCCGTTCA+CGG | 0.441391 | 4.2:+91944439 | MS.gene053162:CDS |
| CGGAGGAGATATTTGCGAAA+TGG | 0.441862 | 4.2:+91944794 | MS.gene053162:CDS |
| ACAAGAGGAAGAAGATGATT+GGG | 0.446433 | 4.2:+91944749 | MS.gene053162:CDS |
| CCGGCAGAGAAAGGCGGAAT+CGG | 0.453844 | 4.2:-91944416 | None:intergenic |
| TTCCGGTGAGCAAGAGCGGC+AGG | 0.464761 | 4.2:-91944572 | None:intergenic |
| GAACGGCGTCGTCGCAGCTC+CGG | 0.476153 | 4.2:-91944435 | None:intergenic |
| GTCGCAGCAGCCACCTTCAC+CGG | 0.482296 | 4.2:-91944907 | None:intergenic |
| AGTGCCGGAGATATGAATTT+CGG | 0.485587 | 4.2:-91944524 | None:intergenic |
| GAGAACGTGGAAGTTACTTC+CGG | 0.494776 | 4.2:+91944888 | MS.gene053162:CDS |
| GTCGCAGCTCCGGCAGAGAA+AGG | 0.496029 | 4.2:-91944425 | None:intergenic |
| GTGGCCAAAATCAGCTTAGC+TGG | 0.499961 | 4.2:-91945018 | None:intergenic |
| TTCACCGAAATTCATATCTC+CGG | 0.509514 | 4.2:+91944520 | MS.gene053162:CDS |
| GTGTCGAAATTTGATGAGGT+TGG | 0.526675 | 4.2:+91944978 | MS.gene053162:CDS |
| GCCACACGATCACCGTTCAC+CGG | 0.535889 | 4.2:-91944841 | None:intergenic |
| AACCTGCCGCTCTTGCTCAC+CGG | 0.536391 | 4.2:+91944570 | MS.gene053162:CDS |
| CGATGATACATCTCCGGCAC+CGG | 0.539008 | 4.2:+91944717 | MS.gene053162:CDS |
| GAACGGTGATCGTGTGGCGG+TGG | 0.541705 | 4.2:+91944846 | MS.gene053162:CDS |
| TGAGGTTGGAGAAAATATCA+GGG | 0.543347 | 4.2:+91944992 | MS.gene053162:CDS |
| TCGTGTGGCGGTGGAAGCTA+TGG | 0.543504 | 4.2:+91944855 | MS.gene053162:CDS |
| CAGAGAAAGGCGGAATCGGA+AGG | 0.554491 | 4.2:-91944412 | None:intergenic |
| ACGGTGTTGTAGTGTAGTGC+CGG | 0.554858 | 4.2:-91944539 | None:intergenic |
| AACACCTTGAATTGCAAGAA+GGG | 0.556767 | 4.2:+91945070 | MS.gene053162:CDS |
| TCACCGGAAAATCATTCCTC+CGG | 0.557681 | 4.2:+91944586 | MS.gene053162:CDS |
| TGAAAGTGTTGGATCGGTGG+CGG | 0.559970 | 4.2:+91944774 | MS.gene053162:CDS |
| AAGTGTTGGATCGGTGGCGG+AGG | 0.560614 | 4.2:+91944777 | MS.gene053162:CDS |
| AGATTGAGAACTCGCCTCAC+CGG | 0.561454 | 4.2:-91944605 | None:intergenic |
| GACTGAAAGTGTTGGATCGG+TGG | 0.563595 | 4.2:+91944771 | MS.gene053162:CDS |
| TCCGGTGAACGGTGATCGTG+TGG | 0.570131 | 4.2:+91944840 | MS.gene053162:CDS |
| CAACACCTTGAATTGCAAGA+AGG | 0.572050 | 4.2:+91945069 | MS.gene053162:CDS |
| GTGGAAGTTACTTCCGGTGA+AGG | 0.577957 | 4.2:+91944894 | MS.gene053162:CDS |
| ATGATTGGGACTGAAAGTGT+TGG | 0.593930 | 4.2:+91944763 | MS.gene053162:CDS |
| TGTTACCGATGATACATCTC+CGG | 0.594095 | 4.2:+91944711 | MS.gene053162:CDS |
| GAAGTTACTTCCGGTGAAGG+TGG | 0.596707 | 4.2:+91944897 | MS.gene053162:CDS |
| GAGGAAGTTAGCGCCGTGAA+CGG | 0.601829 | 4.2:-91944452 | None:intergenic |
| ATCTTGTGCTCCAAATGCGA+CGG | 0.602290 | 4.2:+91944496 | MS.gene053162:CDS |
| AGCGGCAGGTTGATGAGAGA+CGG | 0.602586 | 4.2:-91944558 | None:intergenic |
| CACCGGAGAACAATAACAAG+AGG | 0.613856 | 4.2:+91944734 | MS.gene053162:CDS |
| CCACGTGTCGAAATTTGATG+AGG | 0.617602 | 4.2:+91944974 | MS.gene053162:CDS |
| TGGGACTGAAAGTGTTGGAT+CGG | 0.630097 | 4.2:+91944768 | MS.gene053162:CDS |
| ATTTGGAGCACAAGATGTGA+CGG | 0.631824 | 4.2:-91944489 | None:intergenic |
| GCAGCTCCGGCAGAGAAAGG+CGG | 0.638220 | 4.2:-91944422 | None:intergenic |
| CCTCATCAAATTTCGACACG+TGG | 0.641370 | 4.2:-91944974 | None:intergenic |
| TGTGGCGGTGGAAGCTATGG+CGG | 0.643579 | 4.2:+91944858 | MS.gene053162:CDS |
| TTGAGAACTCGCCTCACCGG+AGG | 0.643923 | 4.2:-91944602 | None:intergenic |
| CGGTGCCGGAGATGTATCAT+CGG | 0.645343 | 4.2:-91944716 | None:intergenic |
| GGTGAACGGTGATCGTGTGG+CGG | 0.678941 | 4.2:+91944843 | MS.gene053162:CDS |
| GGAAAATCATTCCTCCGGTG+AGG | 0.685941 | 4.2:+91944591 | MS.gene053162:CDS |
| ACGTGCGAGTTTCACATGTG+TGG | 0.686351 | 4.2:-91945037 | None:intergenic |
| TGGCGGTGTGTATGAGAACG+TGG | 0.692845 | 4.2:+91944875 | MS.gene053162:CDS |
| GAGGTTGGAGAAAATATCAG+GGG | 0.723090 | 4.2:+91944993 | MS.gene053162:CDS |
| TGGAGCACAAGATGTGACGG+AGG | 0.724489 | 4.2:-91944486 | None:intergenic |
| GACGGAGGTGACGAGCGACG+AGG | 0.727759 | 4.2:-91944471 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAGTTAGAGTTAGATTTTC+CGG | + | chr4.2:91944822-91944841 | MS.gene053162:CDS | 30.0% |
| AACAAGAGGAAGAAGATGAT+TGG | + | chr4.2:91944748-91944767 | MS.gene053162:CDS | 35.0% | |
| AACACCTTGAATTGCAAGAA+GGG | + | chr4.2:91945070-91945089 | MS.gene053162:CDS | 35.0% | |
| ACAAGAGGAAGAAGATGATT+GGG | + | chr4.2:91944749-91944768 | MS.gene053162:CDS | 35.0% | |
| ATGAGGTTGGAGAAAATATC+AGG | + | chr4.2:91944991-91945010 | MS.gene053162:CDS | 35.0% | |
| TGAGGTTGGAGAAAATATCA+GGG | + | chr4.2:91944992-91945011 | MS.gene053162:CDS | 35.0% | |
| TTCACCGAAATTCATATCTC+CGG | + | chr4.2:91944520-91944539 | MS.gene053162:CDS | 35.0% | |
| !! | TTCCTCTTGTTATTGTTCTC+CGG | - | chr4.2:91944739-91944758 | None:intergenic | 35.0% |
| CAACACCTTGAATTGCAAGA+AGG | + | chr4.2:91945069-91945088 | MS.gene053162:CDS | 40.0% | |
| GAGGTTGGAGAAAATATCAG+GGG | + | chr4.2:91944993-91945012 | MS.gene053162:CDS | 40.0% | |
| GTGTCGAAATTTGATGAGGT+TGG | + | chr4.2:91944978-91944997 | MS.gene053162:CDS | 40.0% | |
| TGTTACCGATGATACATCTC+CGG | + | chr4.2:91944711-91944730 | MS.gene053162:CDS | 40.0% | |
| ! | AGTGCCGGAGATATGAATTT+CGG | - | chr4.2:91944527-91944546 | None:intergenic | 40.0% |
| ! | ATGATTGGGACTGAAAGTGT+TGG | + | chr4.2:91944763-91944782 | MS.gene053162:CDS | 40.0% |
| ! | ATTTGGAGCACAAGATGTGA+CGG | - | chr4.2:91944492-91944511 | None:intergenic | 40.0% |
| ! | GAGTTAGATTTTCCGGTGAA+CGG | + | chr4.2:91944829-91944848 | MS.gene053162:CDS | 40.0% |
| ! | GGTTGGGGTTAAGATTTTGT+GGG | + | chr4.2:91944938-91944957 | MS.gene053162:CDS | 40.0% |
| ! | GTTGGGGTTAAGATTTTGTG+GGG | + | chr4.2:91944939-91944958 | MS.gene053162:CDS | 40.0% |
| ! | TGGTTGGGGTTAAGATTTTG+TGG | + | chr4.2:91944937-91944956 | MS.gene053162:CDS | 40.0% |
| ATCTTGTGCTCCAAATGCGA+CGG | + | chr4.2:91944496-91944515 | MS.gene053162:CDS | 45.0% | |
| CACCGGAGAACAATAACAAG+AGG | + | chr4.2:91944734-91944753 | MS.gene053162:CDS | 45.0% | |
| CCACGTGTCGAAATTTGATG+AGG | + | chr4.2:91944974-91944993 | MS.gene053162:CDS | 45.0% | |
| CCTCATCAAATTTCGACACG+TGG | - | chr4.2:91944977-91944996 | None:intergenic | 45.0% | |
| CGGAGGAGATATTTGCGAAA+TGG | + | chr4.2:91944794-91944813 | MS.gene053162:CDS | 45.0% | |
| GAGAACGTGGAAGTTACTTC+CGG | + | chr4.2:91944888-91944907 | MS.gene053162:CDS | 45.0% | |
| TCACCGGAAAATCATTCCTC+CGG | + | chr4.2:91944586-91944605 | MS.gene053162:CDS | 45.0% | |
| ! | TCACCGGAGGAATGATTTTC+CGG | - | chr4.2:91944592-91944611 | None:intergenic | 45.0% |
| ! | TTGTTATTGTTCTCCGGTGC+CGG | - | chr4.2:91944733-91944752 | None:intergenic | 45.0% |
| !! | TGGGACTGAAAGTGTTGGAT+CGG | + | chr4.2:91944768-91944787 | MS.gene053162:CDS | 45.0% |
| ACGTGCGAGTTTCACATGTG+TGG | - | chr4.2:91945040-91945059 | None:intergenic | 50.0% | |
| AGATTGAGAACTCGCCTCAC+CGG | - | chr4.2:91944608-91944627 | None:intergenic | 50.0% | |
| CGCACCCTTCTTGCAATTCA+AGG | - | chr4.2:91945077-91945096 | None:intergenic | 50.0% | |
| GAAGTTACTTCCGGTGAAGG+TGG | + | chr4.2:91944897-91944916 | MS.gene053162:CDS | 50.0% | |
| GGAAAATCATTCCTCCGGTG+AGG | + | chr4.2:91944591-91944610 | MS.gene053162:CDS | 50.0% | |
| GTGGAAGTTACTTCCGGTGA+AGG | + | chr4.2:91944894-91944913 | MS.gene053162:CDS | 50.0% | |
| GTGGCCAAAATCAGCTTAGC+TGG | - | chr4.2:91945021-91945040 | None:intergenic | 50.0% | |
| TTCGGTGAAACCGTCGCATT+TGG | - | chr4.2:91944509-91944528 | None:intergenic | 50.0% | |
| ! | GATTTTCCGGTGAGCAAGAG+CGG | - | chr4.2:91944579-91944598 | None:intergenic | 50.0% |
| !! | ACGGTGTTGTAGTGTAGTGC+CGG | - | chr4.2:91944542-91944561 | None:intergenic | 50.0% |
| !! | GACTGAAAGTGTTGGATCGG+TGG | + | chr4.2:91944771-91944790 | MS.gene053162:CDS | 50.0% |
| !! | GGTGCCAGCTAAGCTGATTT+TGG | + | chr4.2:91945014-91945033 | MS.gene053162:CDS | 50.0% |
| !! | TGAAAGTGTTGGATCGGTGG+CGG | + | chr4.2:91944774-91944793 | MS.gene053162:CDS | 50.0% |
| CAGAGAAAGGCGGAATCGGA+AGG | - | chr4.2:91944415-91944434 | None:intergenic | 55.0% | |
| CGATGATACATCTCCGGCAC+CGG | + | chr4.2:91944717-91944736 | MS.gene053162:CDS | 55.0% | |
| GAGGAAGTTAGCGCCGTGAA+CGG | - | chr4.2:91944455-91944474 | None:intergenic | 55.0% | |
| TGGAGCACAAGATGTGACGG+AGG | - | chr4.2:91944489-91944508 | None:intergenic | 55.0% | |
| TGGCGGTGTGTATGAGAACG+TGG | + | chr4.2:91944875-91944894 | MS.gene053162:CDS | 55.0% | |
| ! | CGGTGCCGGAGATGTATCAT+CGG | - | chr4.2:91944719-91944738 | None:intergenic | 55.0% |
| !! | AGCGGCAGGTTGATGAGAGA+CGG | - | chr4.2:91944561-91944580 | None:intergenic | 55.0% |
| !! | TGCTGCGACGTCGTTTTGGT+TGG | + | chr4.2:91944921-91944940 | MS.gene053162:CDS | 55.0% |
| !!! | CTGCGACGTCGTTTTGGTTG+GGG | + | chr4.2:91944923-91944942 | MS.gene053162:CDS | 55.0% |
| !!! | GCTGCGACGTCGTTTTGGTT+GGG | + | chr4.2:91944922-91944941 | MS.gene053162:CDS | 55.0% |
| AACCTGCCGCTCTTGCTCAC+CGG | + | chr4.2:91944570-91944589 | MS.gene053162:CDS | 60.0% | |
| CCGATTCCGCCTTTCTCTGC+CGG | + | chr4.2:91944416-91944435 | MS.gene053162:CDS | 60.0% | |
| CCGGCAGAGAAAGGCGGAAT+CGG | - | chr4.2:91944419-91944438 | None:intergenic | 60.0% | |
| GCCACACGATCACCGTTCAC+CGG | - | chr4.2:91944844-91944863 | None:intergenic | 60.0% | |
| GGTGAACGGTGATCGTGTGG+CGG | + | chr4.2:91944843-91944862 | MS.gene053162:CDS | 60.0% | |
| TCCGGTGAACGGTGATCGTG+TGG | + | chr4.2:91944840-91944859 | MS.gene053162:CDS | 60.0% | |
| TCGTGTGGCGGTGGAAGCTA+TGG | + | chr4.2:91944855-91944874 | MS.gene053162:CDS | 60.0% | |
| TGTGGCGGTGGAAGCTATGG+CGG | + | chr4.2:91944858-91944877 | MS.gene053162:CDS | 60.0% | |
| TTGAGAACTCGCCTCACCGG+AGG | - | chr4.2:91944605-91944624 | None:intergenic | 60.0% | |
| ! | TGGCTGCTGCGACGTCGTTT+TGG | + | chr4.2:91944917-91944936 | MS.gene053162:CDS | 60.0% |
| !! | AAGTGTTGGATCGGTGGCGG+AGG | + | chr4.2:91944777-91944796 | MS.gene053162:CDS | 60.0% |
| AGCTGCGACGACGCCGTTCA+CGG | + | chr4.2:91944439-91944458 | MS.gene053162:CDS | 65.0% | |
| GAACGGTGATCGTGTGGCGG+TGG | + | chr4.2:91944846-91944865 | MS.gene053162:CDS | 65.0% | |
| GCAGCTCCGGCAGAGAAAGG+CGG | - | chr4.2:91944425-91944444 | None:intergenic | 65.0% | |
| GTCGCAGCAGCCACCTTCAC+CGG | - | chr4.2:91944910-91944929 | None:intergenic | 65.0% | |
| GTCGCAGCTCCGGCAGAGAA+AGG | - | chr4.2:91944428-91944447 | None:intergenic | 65.0% | |
| TTCCGGTGAGCAAGAGCGGC+AGG | - | chr4.2:91944575-91944594 | None:intergenic | 65.0% | |
| GAACGGCGTCGTCGCAGCTC+CGG | - | chr4.2:91944438-91944457 | None:intergenic | 70.0% | |
| GACGGAGGTGACGAGCGACG+AGG | - | chr4.2:91944474-91944493 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 91944355 | 91945107 | 91944355 | ID=MS.gene053162 |
| chr4.2 | mRNA | 91944355 | 91945107 | 91944355 | ID=MS.gene053162.t1;Parent=MS.gene053162 |
| chr4.2 | exon | 91944355 | 91945107 | 91944355 | ID=MS.gene053162.t1.exon1;Parent=MS.gene053162.t1 |
| chr4.2 | CDS | 91944355 | 91945107 | 91944355 | ID=cds.MS.gene053162.t1;Parent=MS.gene053162.t1 |
| Gene Sequence |
| Protein sequence |
