Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055618.t1 | XP_013467691.1 | 97.1 | 204 | 3 | 3 | 1 | 201 | 1 | 204 | 2.30E-101 | 378.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055618.t1 | P46665 | 41.8 | 251 | 85 | 6 | 1 | 201 | 1 | 240 | 3.4e-29 | 129.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055618.t1 | A0A072VJV2 | 97.1 | 204 | 3 | 3 | 1 | 201 | 1 | 204 | 1.7e-101 | 378.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene055618.t1 | TF | HB-other |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055616 | MS.gene055618 | 0.833416 | 5.40E-56 | -1.69E-46 |
| MS.gene055617 | MS.gene055618 | 0.980991 | 1.63E-151 | -1.69E-46 |
| MS.gene055618 | MS.gene063063 | 0.82642 | 2.74E-54 | -1.69E-46 |
| MS.gene055618 | MS.gene44439 | -0.800036 | 1.74E-48 | -1.69E-46 |
| MS.gene055618 | MS.gene91215 | 0.8135 | 2.48E-51 | -1.69E-46 |
| MS.gene055618 | MS.gene97258 | 0.806833 | 6.79E-50 | -1.69E-46 |
| MS.gene055618 | MS.gene97259 | 0.80317 | 3.96E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055618.t1 | MTR_1g054285 | 97.059 | 204 | 3 | 3 | 1 | 201 | 1 | 204 | 3.78e-141 | 395 |
| MS.gene055618.t1 | MTR_7g093430 | 60.377 | 212 | 71 | 8 | 1 | 201 | 1 | 210 | 8.57e-69 | 212 |
| MS.gene055618.t1 | MTR_2g061030 | 42.366 | 262 | 78 | 10 | 1 | 201 | 1 | 250 | 1.08e-33 | 123 |
| MS.gene055618.t1 | MTR_4g100550 | 53.659 | 123 | 46 | 2 | 81 | 201 | 57 | 170 | 4.45e-33 | 120 |
| MS.gene055618.t1 | MTR_5g013010 | 53.488 | 129 | 39 | 4 | 81 | 201 | 71 | 186 | 1.63e-32 | 116 |
| MS.gene055618.t1 | MTR_5g013010 | 53.488 | 129 | 39 | 4 | 81 | 201 | 71 | 186 | 1.03e-31 | 117 |
| MS.gene055618.t1 | MTR_4g097600 | 80.000 | 65 | 11 | 1 | 139 | 201 | 113 | 177 | 2.94e-28 | 107 |
| MS.gene055618.t1 | MTR_8g006705 | 54.054 | 111 | 45 | 1 | 97 | 201 | 86 | 196 | 2.18e-25 | 100 |
| MS.gene055618.t1 | MTR_5g014890 | 75.410 | 61 | 14 | 1 | 142 | 201 | 113 | 173 | 2.02e-23 | 94.4 |
| MS.gene055618.t1 | MTR_1g017090 | 44.444 | 135 | 55 | 5 | 79 | 201 | 54 | 180 | 5.24e-21 | 88.2 |
| MS.gene055618.t1 | MTR_3g103590 | 68.333 | 60 | 19 | 0 | 142 | 201 | 136 | 195 | 8.54e-21 | 88.2 |
| MS.gene055618.t1 | MTR_4g084750 | 73.684 | 57 | 14 | 1 | 146 | 201 | 59 | 115 | 1.17e-19 | 82.8 |
| MS.gene055618.t1 | MTR_4g126900 | 69.388 | 49 | 15 | 0 | 153 | 201 | 90 | 138 | 1.02e-16 | 75.5 |
| MS.gene055618.t1 | MTR_2g038625 | 57.353 | 68 | 28 | 1 | 134 | 201 | 66 | 132 | 3.95e-16 | 73.9 |
| MS.gene055618.t1 | MTR_2g038580 | 57.353 | 68 | 28 | 1 | 134 | 201 | 66 | 132 | 3.95e-16 | 73.9 |
| MS.gene055618.t1 | MTR_4g107650 | 50.602 | 83 | 37 | 2 | 119 | 201 | 67 | 145 | 3.19e-14 | 70.1 |
| MS.gene055618.t1 | MTR_7g010020 | 42.391 | 92 | 52 | 1 | 110 | 201 | 40 | 130 | 6.48e-14 | 68.9 |
| MS.gene055618.t1 | MTR_5g038280 | 48.611 | 72 | 34 | 1 | 130 | 201 | 69 | 137 | 2.83e-12 | 64.7 |
| MS.gene055618.t1 | MTR_8g089895 | 43.617 | 94 | 49 | 2 | 108 | 201 | 15 | 104 | 3.76e-12 | 64.3 |
| MS.gene055618.t1 | MTR_1g061660 | 50.909 | 55 | 27 | 0 | 147 | 201 | 55 | 109 | 6.07e-12 | 62.8 |
| MS.gene055618.t1 | MTR_6g007647 | 58.824 | 51 | 21 | 0 | 151 | 201 | 33 | 83 | 6.26e-12 | 63.2 |
| MS.gene055618.t1 | MTR_6g007647 | 58.824 | 51 | 21 | 0 | 151 | 201 | 67 | 117 | 8.15e-12 | 63.2 |
| MS.gene055618.t1 | MTR_3g086790 | 40.196 | 102 | 59 | 2 | 101 | 201 | 10 | 110 | 9.75e-12 | 62.8 |
| MS.gene055618.t1 | MTR_8g469430 | 54.902 | 51 | 23 | 0 | 151 | 201 | 89 | 139 | 4.18e-11 | 61.2 |
| MS.gene055618.t1 | MTR_5g039000 | 50.847 | 59 | 29 | 0 | 143 | 201 | 69 | 127 | 4.35e-11 | 60.8 |
| MS.gene055618.t1 | MTR_7g103340 | 48.276 | 58 | 30 | 0 | 144 | 201 | 56 | 113 | 5.20e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055618.t1 | AT5G06710 | 41.406 | 256 | 79 | 7 | 1 | 201 | 1 | 240 | 7.50e-40 | 137 |
| MS.gene055618.t1 | AT5G06710 | 41.406 | 256 | 79 | 7 | 1 | 201 | 1 | 240 | 2.98e-39 | 137 |
| MS.gene055618.t1 | AT5G47370 | 48.795 | 166 | 66 | 8 | 48 | 201 | 22 | 180 | 1.76e-34 | 124 |
| MS.gene055618.t1 | AT4G17460 | 44.262 | 183 | 55 | 8 | 48 | 201 | 21 | 185 | 4.64e-30 | 112 |
| MS.gene055618.t1 | AT3G60390 | 51.429 | 140 | 46 | 6 | 81 | 201 | 76 | 212 | 5.64e-30 | 112 |
| MS.gene055618.t1 | AT4G16780 | 55.645 | 124 | 40 | 3 | 81 | 201 | 68 | 179 | 6.88e-28 | 106 |
| MS.gene055618.t1 | AT2G44910 | 48.905 | 137 | 47 | 4 | 81 | 201 | 84 | 213 | 9.67e-27 | 104 |
| MS.gene055618.t1 | AT2G22800 | 76.667 | 60 | 14 | 0 | 142 | 201 | 104 | 163 | 8.97e-25 | 98.2 |
| MS.gene055618.t1 | AT4G37790 | 76.667 | 60 | 14 | 0 | 142 | 201 | 117 | 176 | 2.55e-24 | 97.1 |
| MS.gene055618.t1 | AT5G06710 | 35.652 | 230 | 77 | 7 | 1 | 175 | 1 | 214 | 2.29e-20 | 85.9 |
| MS.gene055618.t1 | AT5G06710 | 35.652 | 230 | 77 | 7 | 1 | 175 | 1 | 214 | 2.29e-20 | 85.9 |
| MS.gene055618.t1 | AT2G01430 | 60.294 | 68 | 27 | 0 | 134 | 201 | 122 | 189 | 2.87e-20 | 85.1 |
| MS.gene055618.t1 | AT2G01430 | 60.294 | 68 | 27 | 0 | 134 | 201 | 122 | 189 | 9.28e-20 | 84.7 |
| MS.gene055618.t1 | AT1G70920 | 65.079 | 63 | 20 | 1 | 141 | 201 | 27 | 89 | 4.51e-17 | 75.5 |
| MS.gene055618.t1 | AT1G70920 | 65.079 | 63 | 20 | 1 | 141 | 201 | 57 | 119 | 7.70e-17 | 75.9 |
| MS.gene055618.t1 | AT2G36610 | 45.312 | 64 | 27 | 1 | 146 | 201 | 66 | 129 | 5.23e-11 | 59.7 |
| MS.gene055618.t1 | AT3G01470 | 56.863 | 51 | 22 | 0 | 151 | 201 | 68 | 118 | 6.77e-11 | 60.5 |
Find 0 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTAAATAAGAAATTTAC+AGG | - | 10841:7076-7095 | MS.gene055618:intergenic | 10.0% |
| !! | AAATATATAAAACCATTTAG+AGG | - | 10841:6438-6457 | MS.gene055618:intergenic | 15.0% |
| !!! | TGAAATGTCAATTTTTTTAA+CGG | + | 10841:6634-6653 | MS.gene055618:intron | 15.0% |
| !!! | TTGTTTCTTCTTTTTAATTA+AGG | + | 10841:7108-7127 | MS.gene055618:intron | 15.0% |
| !! | ATAGTAGATAGAAAATTACA+TGG | - | 10841:6401-6420 | MS.gene055618:intergenic | 20.0% |
| !!! | TTTTTGTTTTTGTTATCATC+AGG | + | 10841:6761-6780 | MS.gene055618:intron | 20.0% |
| ! | AGATAGAAAATTACATGGTT+GGG | - | 10841:6396-6415 | MS.gene055618:intergenic | 25.0% |
| ! | TAGATAGAAAATTACATGGT+TGG | - | 10841:6397-6416 | MS.gene055618:intergenic | 25.0% |
| !! | AGAAAAGAGATAGGTAATTT+TGG | - | 10841:6203-6222 | MS.gene055618:intergenic | 25.0% |
| !! | ATCATTTTCTTTCCTCTAAA+TGG | + | 10841:6423-6442 | MS.gene055618:intron | 25.0% |
| !! | TTAAAGTGCTTGAAAATTGT+GGG | + | 10841:6538-6557 | MS.gene055618:intron | 25.0% |
| !! | TTTAAAGTGCTTGAAAATTG+TGG | + | 10841:6537-6556 | MS.gene055618:intron | 25.0% |
| !!! | TCAATTTTTTTAACGGATCT+AGG | + | 10841:6641-6660 | MS.gene055618:intron | 25.0% |
| AAAGCTTCAGAAGAAAAAAG+AGG | + | 10841:6275-6294 | MS.gene055618:CDS | 30.0% | |
| AATAACAATGCTGAATATGG+AGG | + | 10841:6908-6927 | MS.gene055618:CDS | 30.0% | |
| AGAAACAAGAGAGAAAATGA+AGG | + | 10841:6932-6951 | MS.gene055618:CDS | 30.0% | |
| AGGAATAACAATGCTGAATA+TGG | + | 10841:6905-6924 | MS.gene055618:CDS | 30.0% | |
| ATACTGAAATCCATTGCAAA+TGG | - | 10841:6884-6903 | MS.gene055618:intergenic | 30.0% | |
| GAAATTTACAGGATTAAGAG+TGG | - | 10841:7065-7084 | MS.gene055618:intergenic | 30.0% | |
| ! | GAAGTGATGATGATGAAAAT+GGG | + | 10841:6969-6988 | MS.gene055618:CDS | 30.0% |
| !!! | TTGTTTTTGTTATCATCAGG+TGG | + | 10841:6764-6783 | MS.gene055618:intron | 30.0% |
| ATGATGAAAATGGGTCAACT+AGG | + | 10841:6978-6997 | MS.gene055618:CDS | 35.0% | |
| CAAACCTTGAACCAAATCTT+GGG | - | 10841:6576-6595 | MS.gene055618:intergenic | 35.0% | |
| GGTTTGTCAAGAAAAGAGAT+AGG | - | 10841:6212-6231 | MS.gene055618:intergenic | 35.0% | |
| TTGAATTTGCACCCAAGATT+TGG | + | 10841:6562-6581 | MS.gene055618:intron | 35.0% | |
| ! | GGAAGTGATGATGATGAAAA+TGG | + | 10841:6968-6987 | MS.gene055618:CDS | 35.0% |
| ! | TTGCAATGGATTTCAGTATG+AGG | + | 10841:6885-6904 | MS.gene055618:CDS | 35.0% |
| !!! | TTCTGAAGCTTTTCCTTGTT+GGG | - | 10841:6267-6286 | MS.gene055618:intergenic | 35.0% |
| AACCAAACTTCAACTTGACG+AGG | - | 10841:7213-7232 | MS.gene055618:intergenic | 40.0% | |
| AATGAAGGTGAAGCTGAAAG+AGG | + | 10841:6947-6966 | MS.gene055618:CDS | 40.0% | |
| AGAAGGTCTAGTTGAATAGG+TGG | - | 10841:6311-6330 | MS.gene055618:intergenic | 40.0% | |
| AGTTTCTTCTCAACCCAACA+AGG | + | 10841:6251-6270 | MS.gene055618:CDS | 40.0% | |
| GATCTAAGAACAGGAGAGAA+AGG | - | 10841:6335-6354 | MS.gene055618:intergenic | 40.0% | |
| GCAAACCTTGAACCAAATCT+TGG | - | 10841:6577-6596 | MS.gene055618:intergenic | 40.0% | |
| GGAAGAAGGTCTAGTTGAAT+AGG | - | 10841:6314-6333 | MS.gene055618:intergenic | 40.0% | |
| GTAGAAGCAAAAACTTGCAC+TGG | + | 10841:7167-7186 | MS.gene055618:intron | 40.0% | |
| GTTGAGAAGAAACTTGGTAG+TGG | - | 10841:6245-6264 | MS.gene055618:intergenic | 40.0% | |
| TTCTGTGTCACCATTTGCAA+TGG | + | 10841:6871-6890 | MS.gene055618:CDS | 40.0% | |
| ! | ATGGAACTTGCTTTGAGCTT+AGG | + | 10841:6170-6189 | MS.gene055618:CDS | 40.0% |
| ! | TTTGTTATCATCAGGTGGAC+CGG | + | 10841:6769-6788 | MS.gene055618:intron | 40.0% |
| !! | ACTTGGTAGTGGATCTTTGT+TGG | - | 10841:6234-6253 | MS.gene055618:intergenic | 40.0% |
| !! | CTTGGTAGTGGATCTTTGTT+GGG | - | 10841:6233-6252 | MS.gene055618:intergenic | 40.0% |
| !! | TGTTGGGTTGAGAAGAAACT+TGG | - | 10841:6251-6270 | MS.gene055618:intergenic | 40.0% |
| !!! | CTTCTGAAGCTTTTCCTTGT+TGG | - | 10841:6268-6287 | MS.gene055618:intergenic | 40.0% |
| AAATGGTGACACAGAACTGC+TGG | - | 10841:6867-6886 | MS.gene055618:intergenic | 45.0% | |
| AATGGTGACACAGAACTGCT+GGG | - | 10841:6866-6885 | MS.gene055618:intergenic | 45.0% | |
| GAACAGGAGAGAAAGGAAGA+AGG | - | 10841:6328-6347 | MS.gene055618:intergenic | 45.0% | |
| ! | TGCACCCAAGATTTGGTTCA+AGG | + | 10841:6569-6588 | MS.gene055618:intron | 45.0% |
| !! | GTCCTCGTCAAGTTGAAGTT+TGG | + | 10841:7208-7227 | MS.gene055618:CDS | 45.0% |
| ATGGTGACACAGAACTGCTG+GGG | - | 10841:6865-6884 | MS.gene055618:intergenic | 50.0% | |
| GAATGGGGAGGTGAGAAGAA+GGG | - | 10841:6364-6383 | MS.gene055618:intergenic | 50.0% | |
| GGGTGAGGAGATCTAAGAAC+AGG | - | 10841:6344-6363 | MS.gene055618:intergenic | 50.0% | |
| TTACATGGTTGGGTGAGCCA+AGG | - | 10841:6386-6405 | MS.gene055618:intergenic | 50.0% | |
| ! | GCTAGACGTGAACCGTTTTC+CGG | + | 10841:6799-6818 | MS.gene055618:CDS | 50.0% |
| ACTGCTGGGGGATGACAATG+AGG | - | 10841:6852-6871 | MS.gene055618:intergenic | 55.0% | |
| ATCATCAGGTGGACCGGTGA+GGG | + | 10841:6775-6794 | MS.gene055618:intron | 55.0% | |
| CTTCTCACCTCCCCATTCCT+TGG | + | 10841:6366-6385 | MS.gene055618:CDS | 55.0% | |
| GGAATGGGGAGGTGAGAAGA+AGG | - | 10841:6365-6384 | MS.gene055618:intergenic | 55.0% | |
| GGTTGGGTGAGCCAAGGAAT+GGG | - | 10841:6380-6399 | MS.gene055618:intergenic | 55.0% | |
| GTTGGGTGAGCCAAGGAATG+GGG | - | 10841:6379-6398 | MS.gene055618:intergenic | 55.0% | |
| TATCATCAGGTGGACCGGTG+AGG | + | 10841:6774-6793 | MS.gene055618:intron | 55.0% | |
| TGGTGACACAGAACTGCTGG+GGG | - | 10841:6864-6883 | MS.gene055618:intergenic | 55.0% | |
| TGGTTGGGTGAGCCAAGGAA+TGG | - | 10841:6381-6400 | MS.gene055618:intergenic | 55.0% | |
| ! | TTCACGTCTAGCTCCCTCAC+CGG | - | 10841:6791-6810 | MS.gene055618:intergenic | 55.0% |
| ACGACCGAAGACGTCGACGA+CGG | + | 10841:6824-6843 | MS.gene055618:CDS | 60.0% | |
| CTTCGGTCGTCGCCGGAAAA+CGG | - | 10841:6814-6833 | MS.gene055618:intergenic | 60.0% | |
| GGGAGGTGAGAAGAAGGGTG+AGG | - | 10841:6359-6378 | MS.gene055618:intergenic | 60.0% | |
| GGTACCGTCGTCGACGTCTT+CGG | - | 10841:6831-6850 | MS.gene055618:intergenic | 60.0% | |
| GGGTGAGCCAAGGAATGGGG+AGG | - | 10841:6376-6395 | MS.gene055618:intergenic | 65.0% | |
| TCGACGTCTTCGGTCGTCGC+CGG | - | 10841:6821-6840 | MS.gene055618:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 10841 | gene | 6170 | 7250 | 6170 | ID=MS.gene055618 |
| 10841 | mRNA | 6170 | 7250 | 6170 | ID=MS.gene055618.t1;Parent=MS.gene055618 |
| 10841 | exon | 6170 | 6401 | 6170 | ID=MS.gene055618.t1.exon1;Parent=MS.gene055618.t1 |
| 10841 | CDS | 6170 | 6401 | 6170 | ID=cds.MS.gene055618.t1;Parent=MS.gene055618.t1 |
| 10841 | exon | 6783 | 7075 | 6783 | ID=MS.gene055618.t1.exon2;Parent=MS.gene055618.t1 |
| 10841 | CDS | 6783 | 7075 | 6783 | ID=cds.MS.gene055618.t1;Parent=MS.gene055618.t1 |
| 10841 | exon | 7171 | 7250 | 7171 | ID=MS.gene055618.t1.exon3;Parent=MS.gene055618.t1 |
| 10841 | CDS | 7171 | 7250 | 7171 | ID=cds.MS.gene055618.t1;Parent=MS.gene055618.t1 |
| Gene Sequence |
| Protein sequence |
