AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055617


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055617.t1 MTR_1g054285 97.059 204 3 3 1 201 1 204 3.78e-141 395
MS.gene055617.t1 MTR_7g093430 60.377 212 71 8 1 201 1 210 8.57e-69 212
MS.gene055617.t1 MTR_2g061030 42.366 262 78 10 1 201 1 250 1.08e-33 123
MS.gene055617.t1 MTR_4g100550 53.659 123 46 2 81 201 57 170 4.45e-33 120
MS.gene055617.t1 MTR_5g013010 53.488 129 39 4 81 201 71 186 1.63e-32 116
MS.gene055617.t1 MTR_5g013010 53.488 129 39 4 81 201 71 186 1.03e-31 117
MS.gene055617.t1 MTR_4g097600 80.000 65 11 1 139 201 113 177 2.94e-28 107
MS.gene055617.t1 MTR_8g006705 54.054 111 45 1 97 201 86 196 2.18e-25 100
MS.gene055617.t1 MTR_5g014890 75.410 61 14 1 142 201 113 173 2.02e-23 94.4
MS.gene055617.t1 MTR_1g017090 44.444 135 55 5 79 201 54 180 5.24e-21 88.2
MS.gene055617.t1 MTR_3g103590 68.333 60 19 0 142 201 136 195 8.54e-21 88.2
MS.gene055617.t1 MTR_4g084750 73.684 57 14 1 146 201 59 115 1.17e-19 82.8
MS.gene055617.t1 MTR_4g126900 69.388 49 15 0 153 201 90 138 1.02e-16 75.5
MS.gene055617.t1 MTR_2g038625 57.353 68 28 1 134 201 66 132 3.95e-16 73.9
MS.gene055617.t1 MTR_2g038580 57.353 68 28 1 134 201 66 132 3.95e-16 73.9
MS.gene055617.t1 MTR_4g107650 50.602 83 37 2 119 201 67 145 3.19e-14 70.1
MS.gene055617.t1 MTR_7g010020 42.391 92 52 1 110 201 40 130 6.48e-14 68.9
MS.gene055617.t1 MTR_5g038280 48.611 72 34 1 130 201 69 137 2.83e-12 64.7
MS.gene055617.t1 MTR_8g089895 43.617 94 49 2 108 201 15 104 3.76e-12 64.3
MS.gene055617.t1 MTR_1g061660 50.909 55 27 0 147 201 55 109 6.07e-12 62.8
MS.gene055617.t1 MTR_6g007647 58.824 51 21 0 151 201 33 83 6.26e-12 63.2
MS.gene055617.t1 MTR_6g007647 58.824 51 21 0 151 201 67 117 8.15e-12 63.2
MS.gene055617.t1 MTR_3g086790 40.196 102 59 2 101 201 10 110 9.75e-12 62.8
MS.gene055617.t1 MTR_8g469430 54.902 51 23 0 151 201 89 139 4.18e-11 61.2
MS.gene055617.t1 MTR_5g039000 50.847 59 29 0 143 201 69 127 4.35e-11 60.8
MS.gene055617.t1 MTR_7g103340 48.276 58 30 0 144 201 56 113 5.20e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055617.t1 AT5G06710 41.406 256 79 7 1 201 1 240 7.50e-40 137
MS.gene055617.t1 AT5G06710 41.406 256 79 7 1 201 1 240 2.98e-39 137
MS.gene055617.t1 AT5G47370 48.795 166 66 8 48 201 22 180 1.76e-34 124
MS.gene055617.t1 AT4G17460 44.262 183 55 8 48 201 21 185 4.64e-30 112
MS.gene055617.t1 AT3G60390 51.429 140 46 6 81 201 76 212 5.64e-30 112
MS.gene055617.t1 AT4G16780 55.645 124 40 3 81 201 68 179 6.88e-28 106
MS.gene055617.t1 AT2G44910 48.905 137 47 4 81 201 84 213 9.67e-27 104
MS.gene055617.t1 AT2G22800 76.667 60 14 0 142 201 104 163 8.97e-25 98.2
MS.gene055617.t1 AT4G37790 76.667 60 14 0 142 201 117 176 2.55e-24 97.1
MS.gene055617.t1 AT5G06710 35.652 230 77 7 1 175 1 214 2.29e-20 85.9
MS.gene055617.t1 AT5G06710 35.652 230 77 7 1 175 1 214 2.29e-20 85.9
MS.gene055617.t1 AT2G01430 60.294 68 27 0 134 201 122 189 2.87e-20 85.1
MS.gene055617.t1 AT2G01430 60.294 68 27 0 134 201 122 189 9.28e-20 84.7
MS.gene055617.t1 AT1G70920 65.079 63 20 1 141 201 27 89 4.51e-17 75.5
MS.gene055617.t1 AT1G70920 65.079 63 20 1 141 201 57 119 7.70e-17 75.9
MS.gene055617.t1 AT2G36610 45.312 64 27 1 146 201 66 129 5.23e-11 59.7
MS.gene055617.t1 AT3G01470 56.863 51 22 0 151 201 68 118 6.77e-11 60.5

Find 50 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAAGAGATAGGTAATTT+TGG 0.264059 1.3:-39109282 None:intergenic
AGGAATAACAATGCTGAATA+TGG 0.265099 1.3:+39109989 MS.gene055617:CDS
GGAAGTGATGATGATGAAAA+TGG 0.308043 1.3:+39110052 MS.gene055617:CDS
CTTCTCACCTCCCCATTCCT+TGG 0.329294 1.3:+39109448 MS.gene055617:CDS
ATGGAACTTGCTTTGAGCTT+AGG 0.368374 1.3:+39109252 MS.gene055617:CDS
CTTGGTAGTGGATCTTTGTT+GGG 0.374601 1.3:-39109312 None:intergenic
GGAAGAAGGTCTAGTTGAAT+AGG 0.392211 1.3:-39109393 None:intergenic
GTCCTCGTCAAGTTGAAGTT+TGG 0.396562 1.3:+39110292 MS.gene055617:CDS
ACTTGGTAGTGGATCTTTGT+TGG 0.408944 1.3:-39109313 None:intergenic
GGTTGGGTGAGCCAAGGAAT+GGG 0.421883 1.3:-39109459 None:intergenic
CTTCGGTCGTCGCCGGAAAA+CGG 0.438226 1.3:-39109895 None:intergenic
TAGATAGAAAATTACATGGT+TGG 0.446528 1.3:-39109476 None:intergenic
TTGCAATGGATTTCAGTATG+AGG 0.457926 1.3:+39109969 MS.gene055617:CDS
AGATAGAAAATTACATGGTT+GGG 0.461778 1.3:-39109475 None:intergenic
TTCTGTGTCACCATTTGCAA+TGG 0.477662 1.3:+39109955 MS.gene055617:CDS
GGGTGAGGAGATCTAAGAAC+AGG 0.479275 1.3:-39109423 None:intergenic
GGAATGGGGAGGTGAGAAGA+AGG 0.480630 1.3:-39109444 None:intergenic
ATACTGAAATCCATTGCAAA+TGG 0.496930 1.3:-39109965 None:intergenic
GATCTAAGAACAGGAGAGAA+AGG 0.503681 1.3:-39109414 None:intergenic
TGGTTGGGTGAGCCAAGGAA+TGG 0.507151 1.3:-39109460 None:intergenic
TCGACGTCTTCGGTCGTCGC+CGG 0.507773 1.3:-39109902 None:intergenic
GGTACCGTCGTCGACGTCTT+CGG 0.509499 1.3:-39109912 None:intergenic
AGAAACAAGAGAGAAAATGA+AGG 0.510237 1.3:+39110016 MS.gene055617:CDS
ATCATCAGGTGGACCGGTGA+GGG 0.515305 1.3:+39109859 MS.gene055617:intron
GGTTTGTCAAGAAAAGAGAT+AGG 0.515910 1.3:-39109291 None:intergenic
AATGAAGGTGAAGCTGAAAG+AGG 0.521199 1.3:+39110031 MS.gene055617:CDS
TTTGTTATCATCAGGTGGAC+CGG 0.521239 1.3:+39109853 MS.gene055617:intron
GAATGGGGAGGTGAGAAGAA+GGG 0.522886 1.3:-39109443 None:intergenic
GAAGTGATGATGATGAAAAT+GGG 0.525282 1.3:+39110053 MS.gene055617:CDS
GGGAGGTGAGAAGAAGGGTG+AGG 0.527359 1.3:-39109438 None:intergenic
AAATGGTGACACAGAACTGC+TGG 0.531470 1.3:-39109948 None:intergenic
TGTTGGGTTGAGAAGAAACT+TGG 0.533644 1.3:-39109330 None:intergenic
ATGATGAAAATGGGTCAACT+AGG 0.551419 1.3:+39110062 MS.gene055617:CDS
GAACAGGAGAGAAAGGAAGA+AGG 0.553836 1.3:-39109407 None:intergenic
GTTGAGAAGAAACTTGGTAG+TGG 0.575951 1.3:-39109324 None:intergenic
GAAATTTACAGGATTAAGAG+TGG 0.579010 1.3:-39110146 None:intergenic
AGAAGGTCTAGTTGAATAGG+TGG 0.598462 1.3:-39109390 None:intergenic
GGTTTCAAAATAGAAGAGCA+AGG 0.607323 1.3:+39110313 MS.gene055617:CDS
AATGGTGACACAGAACTGCT+GGG 0.607777 1.3:-39109947 None:intergenic
AGTTTCTTCTCAACCCAACA+AGG 0.609680 1.3:+39109333 MS.gene055617:CDS
TTACATGGTTGGGTGAGCCA+AGG 0.611815 1.3:-39109465 None:intergenic
TTCACGTCTAGCTCCCTCAC+CGG 0.612339 1.3:-39109872 None:intergenic
AATAACAATGCTGAATATGG+AGG 0.617867 1.3:+39109992 MS.gene055617:CDS
AACCAAACTTCAACTTGACG+AGG 0.618703 1.3:-39110294 None:intergenic
GTTGGGTGAGCCAAGGAATG+GGG 0.619312 1.3:-39109458 None:intergenic
TATCATCAGGTGGACCGGTG+AGG 0.627118 1.3:+39109858 MS.gene055617:intron
ATGGTGACACAGAACTGCTG+GGG 0.640069 1.3:-39109946 None:intergenic
ACGACCGAAGACGTCGACGA+CGG 0.675975 1.3:+39109908 MS.gene055617:CDS
GGGTGAGCCAAGGAATGGGG+AGG 0.690782 1.3:-39109455 None:intergenic
TGGTGACACAGAACTGCTGG+GGG 0.743324 1.3:-39109945 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAAATAAGAAATTTAC+AGG - chr1.3:39110160-39110179 None:intergenic 10.0%
!! AAATATATAAAACCATTTAG+AGG - chr1.3:39109520-39109539 None:intergenic 15.0%
!!! TGAAATGTCAATTTTTTTAA+CGG + chr1.3:39109718-39109737 MS.gene055617:intron 15.0%
!!! TTGTTTCTTCTTTTTAATTA+AGG + chr1.3:39110192-39110211 MS.gene055617:intron 15.0%
!! ATAGTAGATAGAAAATTACA+TGG - chr1.3:39109483-39109502 None:intergenic 20.0%
!!! TTTTTGTTTTTGTTATCATC+AGG + chr1.3:39109845-39109864 MS.gene055617:intron 20.0%
! AGATAGAAAATTACATGGTT+GGG - chr1.3:39109478-39109497 None:intergenic 25.0%
! TAGATAGAAAATTACATGGT+TGG - chr1.3:39109479-39109498 None:intergenic 25.0%
!! AGAAAAGAGATAGGTAATTT+TGG - chr1.3:39109285-39109304 None:intergenic 25.0%
!! ATCATTTTCTTTCCTCTAAA+TGG + chr1.3:39109505-39109524 MS.gene055617:intron 25.0%
!! TTAAAGTGCTTGAAAATTGT+GGG + chr1.3:39109621-39109640 MS.gene055617:intron 25.0%
!! TTTAAAGTGCTTGAAAATTG+TGG + chr1.3:39109620-39109639 MS.gene055617:intron 25.0%
!!! TCAATTTTTTTAACGGATCT+AGG + chr1.3:39109725-39109744 MS.gene055617:intron 25.0%
AAAACCTTGAACCAAATCTT+GGG - chr1.3:39109659-39109678 None:intergenic 30.0%
AAAGCTTCAGAAGAAAAAAG+AGG + chr1.3:39109357-39109376 MS.gene055617:CDS 30.0%
AATAACAATGCTGAATATGG+AGG + chr1.3:39109992-39110011 MS.gene055617:CDS 30.0%
AGAAACAAGAGAGAAAATGA+AGG + chr1.3:39110016-39110035 MS.gene055617:CDS 30.0%
AGGAATAACAATGCTGAATA+TGG + chr1.3:39109989-39110008 MS.gene055617:CDS 30.0%
ATACTGAAATCCATTGCAAA+TGG - chr1.3:39109968-39109987 None:intergenic 30.0%
GAAATTTACAGGATTAAGAG+TGG - chr1.3:39110149-39110168 None:intergenic 30.0%
! GAAGTGATGATGATGAAAAT+GGG + chr1.3:39110053-39110072 MS.gene055617:CDS 30.0%
!!! TTGTTTTTGTTATCATCAGG+TGG + chr1.3:39109848-39109867 MS.gene055617:intron 30.0%
ATGATGAAAATGGGTCAACT+AGG + chr1.3:39110062-39110081 MS.gene055617:CDS 35.0%
CAAAACCTTGAACCAAATCT+TGG - chr1.3:39109660-39109679 None:intergenic 35.0%
GGTTTGTCAAGAAAAGAGAT+AGG - chr1.3:39109294-39109313 None:intergenic 35.0%
TTGAATTTGCACCCAAGATT+TGG + chr1.3:39109645-39109664 MS.gene055617:intron 35.0%
! GGAAGTGATGATGATGAAAA+TGG + chr1.3:39110052-39110071 MS.gene055617:CDS 35.0%
! TTGCAATGGATTTCAGTATG+AGG + chr1.3:39109969-39109988 MS.gene055617:CDS 35.0%
!!! TTCTGAAGCTTTTCCTTGTT+GGG - chr1.3:39109349-39109368 None:intergenic 35.0%
AACCAAACTTCAACTTGACG+AGG - chr1.3:39110297-39110316 None:intergenic 40.0%
AATGAAGGTGAAGCTGAAAG+AGG + chr1.3:39110031-39110050 MS.gene055617:CDS 40.0%
AGAAGGTCTAGTTGAATAGG+TGG - chr1.3:39109393-39109412 None:intergenic 40.0%
AGTTTCTTCTCAACCCAACA+AGG + chr1.3:39109333-39109352 MS.gene055617:CDS 40.0%
GATCTAAGAACAGGAGAGAA+AGG - chr1.3:39109417-39109436 None:intergenic 40.0%
GGAAGAAGGTCTAGTTGAAT+AGG - chr1.3:39109396-39109415 None:intergenic 40.0%
GTAGAAGCAAAAACTTGCAC+TGG + chr1.3:39110251-39110270 MS.gene055617:intron 40.0%
GTTGAGAAGAAACTTGGTAG+TGG - chr1.3:39109327-39109346 None:intergenic 40.0%
TTCTGTGTCACCATTTGCAA+TGG + chr1.3:39109955-39109974 MS.gene055617:CDS 40.0%
! ATGGAACTTGCTTTGAGCTT+AGG + chr1.3:39109252-39109271 MS.gene055617:CDS 40.0%
! TTTGTTATCATCAGGTGGAC+CGG + chr1.3:39109853-39109872 MS.gene055617:intron 40.0%
!! ACTTGGTAGTGGATCTTTGT+TGG - chr1.3:39109316-39109335 None:intergenic 40.0%
!! CTTGGTAGTGGATCTTTGTT+GGG - chr1.3:39109315-39109334 None:intergenic 40.0%
!! TGTTGGGTTGAGAAGAAACT+TGG - chr1.3:39109333-39109352 None:intergenic 40.0%
!!! CTTCTGAAGCTTTTCCTTGT+TGG - chr1.3:39109350-39109369 None:intergenic 40.0%
AAATGGTGACACAGAACTGC+TGG - chr1.3:39109951-39109970 None:intergenic 45.0%
AATGGTGACACAGAACTGCT+GGG - chr1.3:39109950-39109969 None:intergenic 45.0%
GAACAGGAGAGAAAGGAAGA+AGG - chr1.3:39109410-39109429 None:intergenic 45.0%
! TGCACCCAAGATTTGGTTCA+AGG + chr1.3:39109652-39109671 MS.gene055617:intron 45.0%
!! GTCCTCGTCAAGTTGAAGTT+TGG + chr1.3:39110292-39110311 MS.gene055617:CDS 45.0%
ATGGTGACACAGAACTGCTG+GGG - chr1.3:39109949-39109968 None:intergenic 50.0%
GAATGGGGAGGTGAGAAGAA+GGG - chr1.3:39109446-39109465 None:intergenic 50.0%
GGGTGAGGAGATCTAAGAAC+AGG - chr1.3:39109426-39109445 None:intergenic 50.0%
TTACATGGTTGGGTGAGCCA+AGG - chr1.3:39109468-39109487 None:intergenic 50.0%
! GCTAGACGTGAACCGTTTTC+CGG + chr1.3:39109883-39109902 MS.gene055617:CDS 50.0%
ACTGCTGGGGGATGACAATG+AGG - chr1.3:39109936-39109955 None:intergenic 55.0%
ATCATCAGGTGGACCGGTGA+GGG + chr1.3:39109859-39109878 MS.gene055617:intron 55.0%
CTTCTCACCTCCCCATTCCT+TGG + chr1.3:39109448-39109467 MS.gene055617:CDS 55.0%
GGAATGGGGAGGTGAGAAGA+AGG - chr1.3:39109447-39109466 None:intergenic 55.0%
GGTTGGGTGAGCCAAGGAAT+GGG - chr1.3:39109462-39109481 None:intergenic 55.0%
GTTGGGTGAGCCAAGGAATG+GGG - chr1.3:39109461-39109480 None:intergenic 55.0%
TATCATCAGGTGGACCGGTG+AGG + chr1.3:39109858-39109877 MS.gene055617:intron 55.0%
TGGTGACACAGAACTGCTGG+GGG - chr1.3:39109948-39109967 None:intergenic 55.0%
TGGTTGGGTGAGCCAAGGAA+TGG - chr1.3:39109463-39109482 None:intergenic 55.0%
! TTCACGTCTAGCTCCCTCAC+CGG - chr1.3:39109875-39109894 None:intergenic 55.0%
ACGACCGAAGACGTCGACGA+CGG + chr1.3:39109908-39109927 MS.gene055617:CDS 60.0%
CTTCGGTCGTCGCCGGAAAA+CGG - chr1.3:39109898-39109917 None:intergenic 60.0%
GGGAGGTGAGAAGAAGGGTG+AGG - chr1.3:39109441-39109460 None:intergenic 60.0%
GGTACCGTCGTCGACGTCTT+CGG - chr1.3:39109915-39109934 None:intergenic 60.0%
GGGTGAGCCAAGGAATGGGG+AGG - chr1.3:39109458-39109477 None:intergenic 65.0%
TCGACGTCTTCGGTCGTCGC+CGG - chr1.3:39109905-39109924 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 39109252 39110334 39109252 ID=MS.gene055617
chr1.3 mRNA 39109252 39110334 39109252 ID=MS.gene055617.t1;Parent=MS.gene055617
chr1.3 exon 39109252 39109483 39109252 ID=MS.gene055617.t1.exon1;Parent=MS.gene055617.t1
chr1.3 CDS 39109252 39109483 39109252 ID=cds.MS.gene055617.t1;Parent=MS.gene055617.t1
chr1.3 exon 39109867 39110159 39109867 ID=MS.gene055617.t1.exon2;Parent=MS.gene055617.t1
chr1.3 CDS 39109867 39110159 39109867 ID=cds.MS.gene055617.t1;Parent=MS.gene055617.t1
chr1.3 exon 39110255 39110334 39110255 ID=MS.gene055617.t1.exon3;Parent=MS.gene055617.t1
chr1.3 CDS 39110255 39110334 39110255 ID=cds.MS.gene055617.t1;Parent=MS.gene055617.t1
Gene Sequence

>MS.gene055617

ATGGAACTTGCTTTGAGCTTAGGTGACACACCAAAATTACCTATCTCTTTTCTTGACAAACCCAACAAAGATCCACTACCAAGTTTCTTCTCAACCCAACAAGGAAAAGCTTCAGAAGAAAAAAGAGGTTCTTCAGATCCACCTATTCAACTAGACCTTCTTCCTTTCTCTCCTGTTCTTAGATCTCCTCACCCTTCTTCTCACCTCCCCATTCCTTGGCTCACCCAACCATGTAATTTTCTATCTACTATAAATCATTTTCTTTCCTCTAAATGGTTTTATATATTTGTTTAATATCTAGATAAATTTGTTTAATTTTATTTTGTTTTTATAGATTTAATTTTATCTTTTTTGTTTTTTTTTTTGTTTTTAAAGTGCTTGAAAATTGTGGGTTTGAATTTGCACCCAAGATTTGGTTCAAGGTTTTGCATATTCTTGACTAATGCTTATTGAAAAATATTTGTTATGAAATGTCAATTTTTTTAACGGATCTAGGTTACTTCGAGATATTAGTCTTTGCAGTTACGCGCATGAATTCATATTGTTATAAAAAAAAATTGAAAATATATTTAAAGTTGTATATACTCAATCATTTTTTGTTTTTGTTATCATCAGGTGGACCGGTGAGGGAGCTAGACGTGAACCGTTTTCCGGCGACGACCGAAGACGTCGACGACGGTACCTCATTGTCATCCCCCAGCAGTTCTGTGTCACCATTTGCAATGGATTTCAGTATGAGGAATAACAATGCTGAATATGGAGGAAGAAACAAGAGAGAAAATGAAGGTGAAGCTGAAAGAGGAAGTGATGATGATGAAAATGGGTCAACTAGGAAGAAACTAAGACTCTCTAAAGATCAATCTGCTTTTCTTGAAGAAAGTTTCAAAGAACACACCACTCTTAATCCTGTAAATTTCTTATTTAATTTTTGTTTATTTACTTGTTTCTTCTTTTTAATTAAGGTTTATTTGTTAATTAATCATGTTTATTTATTTGCTTGTAGAAGCAAAAACTTGCACTGGCAAAACAGTTGAATCTTAGTCCTCGTCAAGTTGAAGTTTGGTTTCAAAATAGAAGAGCAAG

Protein sequence

>MS.gene055617.t1

MELALSLGDTPKLPISFLDKPNKDPLPSFFSTQQGKASEEKRGSSDPPIQLDLLPFSPVLRSPHPSSHLPIPWLTQPCGPVRELDVNRFPATTEDVDDGTSLSSPSSSVSPFAMDFSMRNNNAEYGGRNKRENEGEAERGSDDDENGSTRKKLRLSKDQSAFLEESFKEHTTLNPKQKLALAKQLNLSPRQVEVWFQNRRA