Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063063.t1 | XP_003622688.1 | 95.8 | 265 | 11 | 0 | 1 | 265 | 1 | 265 | 4.60E-134 | 487.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063063.t1 | P83947 | 43.3 | 270 | 129 | 8 | 3 | 265 | 4 | 256 | 6.9e-46 | 185.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063063.t1 | G7KVX9 | 95.8 | 265 | 11 | 0 | 1 | 265 | 1 | 265 | 3.3e-134 | 487.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene063063 | -0.81305 | 3.12E-51 | -1.69E-46 |
| MS.gene049222 | MS.gene063063 | -0.806287 | 8.85E-50 | -1.69E-46 |
| MS.gene04932 | MS.gene063063 | 0.800531 | 1.38E-48 | -1.69E-46 |
| MS.gene050170 | MS.gene063063 | -0.816121 | 6.51E-52 | -1.69E-46 |
| MS.gene050577 | MS.gene063063 | -0.811402 | 7.13E-51 | -1.69E-46 |
| MS.gene050747 | MS.gene063063 | -0.811721 | 6.08E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene063063 | -0.812612 | 3.88E-51 | -1.69E-46 |
| MS.gene050871 | MS.gene063063 | -0.81113 | 8.17E-51 | -1.69E-46 |
| MS.gene050950 | MS.gene063063 | -0.810306 | 1.23E-50 | -1.69E-46 |
| MS.gene051651 | MS.gene063063 | 0.804888 | 1.74E-49 | -1.69E-46 |
| MS.gene052015 | MS.gene063063 | 0.80517 | 1.52E-49 | -1.69E-46 |
| MS.gene052211 | MS.gene063063 | 0.806462 | 8.13E-50 | -1.69E-46 |
| MS.gene052494 | MS.gene063063 | 0.808111 | 3.64E-50 | -1.69E-46 |
| MS.gene052503 | MS.gene063063 | -0.801342 | 9.42E-49 | -1.69E-46 |
| MS.gene052606 | MS.gene063063 | 0.845239 | 4.60E-59 | -1.69E-46 |
| MS.gene052702 | MS.gene063063 | 0.805057 | 1.60E-49 | -1.69E-46 |
| MS.gene052823 | MS.gene063063 | -0.808562 | 2.91E-50 | -1.69E-46 |
| MS.gene052825 | MS.gene063063 | -0.808256 | 3.39E-50 | -1.69E-46 |
| MS.gene053097 | MS.gene063063 | 0.835215 | 1.91E-56 | -1.69E-46 |
| MS.gene053108 | MS.gene063063 | 0.817336 | 3.48E-52 | -1.69E-46 |
| MS.gene053203 | MS.gene063063 | -0.804425 | 2.17E-49 | -1.69E-46 |
| MS.gene053479 | MS.gene063063 | -0.800487 | 1.41E-48 | -1.69E-46 |
| MS.gene054408 | MS.gene063063 | -0.800241 | 1.58E-48 | -1.69E-46 |
| MS.gene05470 | MS.gene063063 | -0.824077 | 9.81E-54 | -1.69E-46 |
| MS.gene054884 | MS.gene063063 | 0.818498 | 1.90E-52 | -1.69E-46 |
| MS.gene055158 | MS.gene063063 | 0.838486 | 2.79E-57 | -1.69E-46 |
| MS.gene055159 | MS.gene063063 | 0.80054 | 1.37E-48 | -1.69E-46 |
| MS.gene055502 | MS.gene063063 | -0.811021 | 8.62E-51 | -1.69E-46 |
| MS.gene055577 | MS.gene063063 | -0.800498 | 1.40E-48 | -1.69E-46 |
| MS.gene055616 | MS.gene063063 | 0.802363 | 5.81E-49 | -1.69E-46 |
| MS.gene055617 | MS.gene063063 | 0.809888 | 1.51E-50 | -1.69E-46 |
| MS.gene055618 | MS.gene063063 | 0.82642 | 2.74E-54 | -1.69E-46 |
| MS.gene055729 | MS.gene063063 | 0.802273 | 6.07E-49 | -1.69E-46 |
| MS.gene055731 | MS.gene063063 | 0.807819 | 4.20E-50 | -1.69E-46 |
| MS.gene05589 | MS.gene063063 | -0.831564 | 1.55E-55 | -1.69E-46 |
| MS.gene055977 | MS.gene063063 | 0.801622 | 8.26E-49 | -1.69E-46 |
| MS.gene056504 | MS.gene063063 | -0.800316 | 1.53E-48 | -1.69E-46 |
| MS.gene056590 | MS.gene063063 | 0.818975 | 1.48E-52 | -1.69E-46 |
| MS.gene056591 | MS.gene063063 | 0.818639 | 1.76E-52 | -1.69E-46 |
| MS.gene056694 | MS.gene063063 | 0.843054 | 1.77E-58 | -1.69E-46 |
| MS.gene056697 | MS.gene063063 | 0.805715 | 1.17E-49 | -1.69E-46 |
| MS.gene057081 | MS.gene063063 | 0.815252 | 1.02E-51 | -1.69E-46 |
| MS.gene057261 | MS.gene063063 | 0.80908 | 2.26E-50 | -1.69E-46 |
| MS.gene057768 | MS.gene063063 | 0.852925 | 3.35E-61 | -1.69E-46 |
| MS.gene058171 | MS.gene063063 | 0.80578 | 1.13E-49 | -1.69E-46 |
| MS.gene058173 | MS.gene063063 | 0.803201 | 3.90E-49 | -1.69E-46 |
| MS.gene058175 | MS.gene063063 | 0.808597 | 2.86E-50 | -1.69E-46 |
| MS.gene058334 | MS.gene063063 | 0.818539 | 1.86E-52 | -1.69E-46 |
| MS.gene05837 | MS.gene063063 | -0.834012 | 3.83E-56 | -1.69E-46 |
| MS.gene058418 | MS.gene063063 | 0.821812 | 3.31E-53 | -1.69E-46 |
| MS.gene058544 | MS.gene063063 | -0.811806 | 5.82E-51 | -1.69E-46 |
| MS.gene058838 | MS.gene063063 | -0.810772 | 9.76E-51 | -1.69E-46 |
| MS.gene059023 | MS.gene063063 | -0.807006 | 6.24E-50 | -1.69E-46 |
| MS.gene05914 | MS.gene063063 | -0.831285 | 1.82E-55 | -1.69E-46 |
| MS.gene059526 | MS.gene063063 | 0.806031 | 1.00E-49 | -1.69E-46 |
| MS.gene05960 | MS.gene063063 | 0.827801 | 1.28E-54 | -1.69E-46 |
| MS.gene059667 | MS.gene063063 | -0.805397 | 1.36E-49 | -1.69E-46 |
| MS.gene06000 | MS.gene063063 | -0.802982 | 4.33E-49 | -1.69E-46 |
| MS.gene060087 | MS.gene063063 | 0.81792 | 2.57E-52 | -1.69E-46 |
| MS.gene060593 | MS.gene063063 | 0.833948 | 3.98E-56 | -1.69E-46 |
| MS.gene060595 | MS.gene063063 | 0.800778 | 1.23E-48 | -1.69E-46 |
| MS.gene060762 | MS.gene063063 | -0.803455 | 3.46E-49 | -1.69E-46 |
| MS.gene06077 | MS.gene063063 | -0.810064 | 1.39E-50 | -1.69E-46 |
| MS.gene060982 | MS.gene063063 | -0.803665 | 3.13E-49 | -1.69E-46 |
| MS.gene061044 | MS.gene063063 | 0.831933 | 1.26E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063063.t1 | MTR_7g050870 | 95.849 | 265 | 11 | 0 | 1 | 265 | 1 | 265 | 1.28e-175 | 495 |
| MS.gene063063.t1 | MTR_7g050950 | 84.470 | 264 | 41 | 0 | 1 | 264 | 1 | 264 | 1.32e-152 | 437 |
| MS.gene063063.t1 | MTR_7g050980 | 77.820 | 266 | 58 | 1 | 1 | 265 | 1 | 266 | 1.34e-145 | 419 |
| MS.gene063063.t1 | MTR_4g025730 | 31.646 | 237 | 134 | 8 | 34 | 263 | 11 | 226 | 9.51e-24 | 100 |
| MS.gene063063.t1 | MTR_7g108905 | 30.870 | 230 | 137 | 8 | 51 | 264 | 45 | 268 | 1.34e-22 | 97.1 |
| MS.gene063063.t1 | MTR_2g044880 | 39.490 | 157 | 79 | 7 | 117 | 264 | 88 | 237 | 1.18e-21 | 94.4 |
| MS.gene063063.t1 | MTR_1g008140 | 33.668 | 199 | 100 | 6 | 97 | 265 | 123 | 319 | 3.53e-21 | 92.8 |
| MS.gene063063.t1 | MTR_8g023310 | 30.705 | 241 | 120 | 10 | 52 | 263 | 78 | 300 | 1.41e-20 | 91.3 |
| MS.gene063063.t1 | MTR_7g112580 | 28.947 | 228 | 134 | 10 | 55 | 263 | 44 | 262 | 1.14e-19 | 88.6 |
| MS.gene063063.t1 | MTR_3g010780 | 29.646 | 226 | 136 | 8 | 54 | 264 | 74 | 291 | 1.34e-18 | 85.5 |
| MS.gene063063.t1 | MTR_2g436040 | 28.700 | 223 | 142 | 7 | 52 | 265 | 41 | 255 | 2.77e-17 | 81.6 |
| MS.gene063063.t1 | MTR_1g086190 | 28.870 | 239 | 132 | 10 | 54 | 265 | 51 | 278 | 3.53e-17 | 81.3 |
| MS.gene063063.t1 | MTR_4g124550 | 27.542 | 236 | 136 | 8 | 54 | 265 | 84 | 308 | 4.16e-17 | 80.9 |
| MS.gene063063.t1 | MTR_4g120290 | 29.245 | 212 | 119 | 8 | 76 | 263 | 75 | 279 | 5.12e-17 | 80.9 |
| MS.gene063063.t1 | MTR_8g023150 | 27.854 | 219 | 138 | 6 | 54 | 259 | 80 | 291 | 1.02e-16 | 79.7 |
| MS.gene063063.t1 | MTR_3g091380 | 28.571 | 294 | 161 | 11 | 10 | 264 | 25 | 308 | 1.60e-16 | 79.3 |
| MS.gene063063.t1 | MTR_8g042900 | 31.004 | 229 | 144 | 9 | 48 | 264 | 72 | 298 | 2.28e-16 | 79.0 |
| MS.gene063063.t1 | MTR_2g436050 | 27.354 | 223 | 139 | 7 | 54 | 263 | 59 | 271 | 3.63e-16 | 78.2 |
| MS.gene063063.t1 | MTR_4g025690 | 37.419 | 155 | 74 | 7 | 117 | 265 | 92 | 229 | 3.77e-16 | 78.2 |
| MS.gene063063.t1 | MTR_4g120280 | 29.858 | 211 | 119 | 8 | 76 | 263 | 75 | 279 | 8.57e-16 | 77.0 |
| MS.gene063063.t1 | MTR_4g087980 | 33.333 | 174 | 99 | 6 | 99 | 264 | 64 | 228 | 9.18e-16 | 77.0 |
| MS.gene063063.t1 | MTR_7g110440 | 27.236 | 246 | 133 | 10 | 54 | 263 | 45 | 280 | 1.36e-15 | 76.6 |
| MS.gene063063.t1 | MTR_4g087970 | 34.868 | 152 | 86 | 6 | 120 | 263 | 82 | 228 | 1.91e-15 | 75.9 |
| MS.gene063063.t1 | MTR_7g110430 | 27.126 | 247 | 132 | 9 | 54 | 263 | 45 | 280 | 2.17e-15 | 75.9 |
| MS.gene063063.t1 | MTR_1g086210 | 28.804 | 184 | 97 | 6 | 109 | 263 | 96 | 274 | 2.64e-15 | 75.9 |
| MS.gene063063.t1 | MTR_2g436070 | 28.649 | 185 | 117 | 5 | 92 | 263 | 89 | 271 | 3.92e-15 | 74.7 |
| MS.gene063063.t1 | MTR_4g035980 | 34.722 | 144 | 85 | 4 | 124 | 262 | 108 | 247 | 1.56e-14 | 73.6 |
| MS.gene063063.t1 | MTR_7g110490 | 25.610 | 246 | 137 | 10 | 54 | 263 | 45 | 280 | 1.77e-14 | 73.2 |
| MS.gene063063.t1 | MTR_7g110510 | 25.610 | 246 | 137 | 10 | 54 | 263 | 45 | 280 | 1.77e-14 | 73.2 |
| MS.gene063063.t1 | MTR_1g083510 | 31.527 | 203 | 104 | 9 | 92 | 263 | 95 | 293 | 2.19e-14 | 73.2 |
| MS.gene063063.t1 | MTR_7g110420 | 26.016 | 246 | 136 | 10 | 54 | 263 | 45 | 280 | 4.45e-14 | 72.0 |
| MS.gene063063.t1 | MTR_7g108900 | 31.839 | 223 | 109 | 7 | 79 | 265 | 55 | 270 | 1.10e-13 | 70.9 |
| MS.gene063063.t1 | MTR_8g104655 | 27.311 | 238 | 127 | 7 | 47 | 262 | 37 | 250 | 2.32e-13 | 69.7 |
| MS.gene063063.t1 | MTR_3g033690 | 28.077 | 260 | 146 | 12 | 15 | 262 | 4 | 234 | 1.28e-12 | 67.8 |
| MS.gene063063.t1 | MTR_8g104580 | 26.891 | 238 | 128 | 7 | 47 | 262 | 29 | 242 | 1.87e-12 | 67.0 |
| MS.gene063063.t1 | MTR_1g083470 | 30.247 | 162 | 97 | 6 | 116 | 263 | 119 | 278 | 6.55e-12 | 65.5 |
| MS.gene063063.t1 | MTR_1g083500 | 30.247 | 162 | 97 | 6 | 116 | 263 | 119 | 278 | 6.55e-12 | 65.5 |
| MS.gene063063.t1 | MTR_3g008720 | 33.333 | 147 | 79 | 7 | 123 | 262 | 101 | 235 | 1.14e-11 | 64.7 |
| MS.gene063063.t1 | MTR_2g044810 | 28.571 | 189 | 113 | 7 | 83 | 265 | 76 | 248 | 1.95e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063063.t1 | AT1G11580 | 41.026 | 273 | 145 | 6 | 3 | 265 | 4 | 270 | 2.23e-50 | 174 |
| MS.gene063063.t1 | AT1G11580 | 41.026 | 273 | 145 | 6 | 3 | 265 | 25 | 291 | 4.17e-50 | 173 |
| MS.gene063063.t1 | AT1G11590 | 36.123 | 227 | 118 | 9 | 56 | 265 | 23 | 239 | 1.45e-23 | 99.8 |
| MS.gene063063.t1 | AT5G53370 | 32.065 | 184 | 108 | 5 | 97 | 265 | 152 | 333 | 3.68e-21 | 93.2 |
| MS.gene063063.t1 | AT5G53370 | 32.065 | 184 | 108 | 5 | 97 | 265 | 116 | 297 | 4.37e-21 | 92.8 |
| MS.gene063063.t1 | AT3G49220 | 33.133 | 166 | 90 | 5 | 116 | 265 | 193 | 353 | 6.66e-20 | 88.6 |
| MS.gene063063.t1 | AT4G03930 | 33.043 | 230 | 121 | 10 | 56 | 265 | 23 | 239 | 6.96e-20 | 89.0 |
| MS.gene063063.t1 | AT3G49220 | 33.133 | 166 | 90 | 5 | 116 | 265 | 193 | 353 | 2.10e-19 | 87.8 |
| MS.gene063063.t1 | AT3G49220 | 33.133 | 166 | 90 | 5 | 116 | 265 | 148 | 308 | 3.22e-19 | 87.4 |
| MS.gene063063.t1 | AT5G27870 | 32.099 | 162 | 93 | 4 | 116 | 263 | 116 | 274 | 1.55e-18 | 85.5 |
| MS.gene063063.t1 | AT3G59010 | 37.179 | 156 | 73 | 5 | 122 | 261 | 102 | 248 | 4.09e-18 | 84.0 |
| MS.gene063063.t1 | AT2G45220 | 39.073 | 151 | 72 | 7 | 117 | 262 | 92 | 227 | 4.43e-17 | 80.9 |
| MS.gene063063.t1 | AT3G27980 | 42.982 | 114 | 55 | 5 | 155 | 265 | 106 | 212 | 4.72e-17 | 80.9 |
| MS.gene063063.t1 | AT3G05610 | 28.916 | 166 | 93 | 5 | 117 | 263 | 118 | 277 | 3.79e-16 | 78.2 |
| MS.gene063063.t1 | AT2G26440 | 29.258 | 229 | 146 | 6 | 49 | 264 | 35 | 260 | 2.33e-15 | 75.9 |
| MS.gene063063.t1 | AT3G43270 | 28.141 | 199 | 108 | 7 | 87 | 264 | 53 | 237 | 2.99e-15 | 75.5 |
| MS.gene063063.t1 | AT4G33220 | 34.483 | 174 | 86 | 7 | 117 | 264 | 64 | 235 | 3.25e-15 | 75.5 |
| MS.gene063063.t1 | AT5G51500 | 34.194 | 155 | 79 | 7 | 117 | 259 | 100 | 243 | 6.04e-14 | 71.6 |
| MS.gene063063.t1 | AT4G02320 | 31.138 | 167 | 91 | 6 | 115 | 264 | 71 | 230 | 1.21e-13 | 70.9 |
| MS.gene063063.t1 | AT1G53830 | 29.918 | 244 | 129 | 11 | 54 | 264 | 65 | 299 | 6.21e-13 | 68.6 |
| MS.gene063063.t1 | AT2G43050 | 34.694 | 147 | 78 | 6 | 122 | 261 | 98 | 233 | 7.13e-12 | 65.5 |
| MS.gene063063.t1 | AT2G26450 | 26.794 | 209 | 124 | 6 | 76 | 264 | 125 | 324 | 8.35e-12 | 65.5 |
| MS.gene063063.t1 | AT4G02300 | 30.247 | 162 | 91 | 6 | 118 | 264 | 90 | 244 | 1.26e-11 | 64.7 |
Find 58 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGAGATGAGAAATAATA+AGG | 0.234584 | 7.3:+53364784 | None:intergenic |
| GAATGACTGTGAGGAATTAA+TGG | 0.257485 | 7.3:-53364517 | MS.gene063063:CDS |
| TGTACTCAATTTGTGGTCTT+TGG | 0.308331 | 7.3:+53364644 | None:intergenic |
| ATTTGTCCATGGATAGAGTT+TGG | 0.321286 | 7.3:-53364494 | MS.gene063063:CDS |
| GAGAGATTGAAGAAGGAGAT+AGG | 0.326855 | 7.3:+53364759 | None:intergenic |
| AAGTAAGGATCGAAGGCTTT+TGG | 0.339991 | 7.3:-53364211 | MS.gene063063:CDS |
| AACTTGCTTGGATGGATTAG+AGG | 0.346392 | 7.3:-53364385 | MS.gene063063:CDS |
| TTTGTCCATGGATAGAGTTT+GGG | 0.389169 | 7.3:-53364493 | MS.gene063063:CDS |
| ATGAATCCGTCGTTAGCCTT+TGG | 0.389233 | 7.3:+53364273 | None:intergenic |
| TCGAATGTGTTCAGTCGATT+TGG | 0.391545 | 7.3:+53364605 | None:intergenic |
| TACCTTGTCTTACCGTTGTC+TGG | 0.395409 | 7.3:+53364096 | None:intergenic |
| GGGTTCATCTCGGGTCGTTA+TGG | 0.403997 | 7.3:-53364364 | MS.gene063063:CDS |
| ATCTTCTGTAGGGGACATAA+AGG | 0.421877 | 7.3:-53364187 | MS.gene063063:CDS |
| CGTTATGGAGAGTAACCTTC+AGG | 0.424231 | 7.3:-53364349 | MS.gene063063:CDS |
| TCCATCTTGGATGACAAGTA+AGG | 0.431689 | 7.3:-53364226 | MS.gene063063:CDS |
| CACTAACCATGCAACTTGCT+TGG | 0.434494 | 7.3:-53364397 | MS.gene063063:CDS |
| AACCATGCAACTTGCTTGGA+TGG | 0.443880 | 7.3:-53364393 | MS.gene063063:CDS |
| GGCCAATGTTGTGGTGGCTC+AGG | 0.444253 | 7.3:-53364166 | MS.gene063063:CDS |
| GCTTGTCTAGCAAAGATTGT+TGG | 0.453804 | 7.3:+53364874 | None:intergenic |
| ATATGAGTGTACTCAATTTG+TGG | 0.460078 | 7.3:+53364637 | None:intergenic |
| AGAGCATGCTCACATAGATT+AGG | 0.476252 | 7.3:+53364729 | None:intergenic |
| TGTTTCTGTCTTGCCTCCAA+AGG | 0.485356 | 7.3:-53364289 | MS.gene063063:CDS |
| ACCTTCAGGACTTGATCTCC+AGG | 0.485514 | 7.3:-53364335 | MS.gene063063:CDS |
| AGCTGAGGAGAGATTGAAGA+AGG | 0.490290 | 7.3:+53364752 | None:intergenic |
| GTTGAGATGAGAAATAATAA+GGG | 0.504890 | 7.3:+53364785 | None:intergenic |
| CCTCTCTAGGGCTATTAACA+CGG | 0.513251 | 7.3:+53364550 | None:intergenic |
| TCCTTACTTGTCATCCAAGA+TGG | 0.513804 | 7.3:+53364225 | None:intergenic |
| AATCCGTCGTTAGCCTTTGG+AGG | 0.514243 | 7.3:+53364276 | None:intergenic |
| ATGTTGTGGTGGCTCAGGAC+GGG | 0.518219 | 7.3:-53364161 | MS.gene063063:CDS |
| TGGATTAGAGGGTTCATCTC+GGG | 0.520702 | 7.3:-53364373 | MS.gene063063:CDS |
| CATGTATCAGAAGTGGTTCA+AGG | 0.525478 | 7.3:-53364684 | MS.gene063063:CDS |
| GGAATTAATGGATTTGTCCA+TGG | 0.534928 | 7.3:-53364505 | MS.gene063063:CDS |
| ACTTGCTTGGATGGATTAGA+GGG | 0.551910 | 7.3:-53364384 | MS.gene063063:CDS |
| AATGTTGTGGTGGCTCAGGA+CGG | 0.552902 | 7.3:-53364162 | MS.gene063063:CDS |
| GTGGTGGCTCAGGACGGGAG+CGG | 0.558457 | 7.3:-53364156 | MS.gene063063:CDS |
| CTTAACTCATGTATCAGAAG+TGG | 0.560141 | 7.3:-53364691 | MS.gene063063:CDS |
| ATGGATTAGAGGGTTCATCT+CGG | 0.561420 | 7.3:-53364374 | MS.gene063063:CDS |
| CCCTGGAGATCAAGTCCTGA+AGG | 0.569086 | 7.3:+53364334 | None:intergenic |
| AGCAAGAGAAGATCTAGCCC+TGG | 0.572507 | 7.3:+53364317 | None:intergenic |
| TGAGGCCCAAACTCTATCCA+TGG | 0.582316 | 7.3:+53364488 | None:intergenic |
| TGAACACATTCGAAAGGTCA+TGG | 0.584603 | 7.3:-53364595 | MS.gene063063:CDS |
| GTCCTGAGCCACCACAACAT+TGG | 0.587886 | 7.3:+53364164 | None:intergenic |
| CACAGTATGATGCACACACA+TGG | 0.596719 | 7.3:-53364434 | MS.gene063063:CDS |
| GGACATAAAGGCCAATGTTG+TGG | 0.601608 | 7.3:-53364175 | MS.gene063063:CDS |
| CATAAAGGCCAATGTTGTGG+TGG | 0.610350 | 7.3:-53364172 | MS.gene063063:CDS |
| GGATGACAAGTAAGGATCGA+AGG | 0.612917 | 7.3:-53364218 | MS.gene063063:CDS |
| CTCACATAGATTAGGAGCTG+AGG | 0.623504 | 7.3:+53364737 | None:intergenic |
| ATCCATCCAAGCAAGTTGCA+TGG | 0.627349 | 7.3:+53364391 | None:intergenic |
| ATCGACTGAACACATTCGAA+AGG | 0.632449 | 7.3:-53364601 | MS.gene063063:CDS |
| CATTCGAAAGGTCATGGACA+CGG | 0.638521 | 7.3:-53364589 | MS.gene063063:CDS |
| CCGTGTTAATAGCCCTAGAG+AGG | 0.641594 | 7.3:-53364550 | MS.gene063063:CDS |
| TTATGATAGCTACTAAAGAG+AGG | 0.651061 | 7.3:+53364817 | None:intergenic |
| GTTGCATCTGCACCAGACAA+CGG | 0.656621 | 7.3:-53364108 | None:intergenic |
| CCTTCAGGACTTGATCTCCA+GGG | 0.682424 | 7.3:-53364334 | MS.gene063063:CDS |
| TTGCCTCCAAAGGCTAACGA+CGG | 0.696925 | 7.3:-53364279 | MS.gene063063:CDS |
| GAGCGGCAAGTTCAAGACTG+TGG | 0.711595 | 7.3:-53364139 | MS.gene063063:CDS |
| AAATGCTTTGAATGACTGTG+AGG | 0.716518 | 7.3:-53364526 | MS.gene063063:CDS |
| TTTGGTTAGAGTCAACACTG+AGG | 0.792892 | 7.3:+53364470 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTCATTGATGAAAAATTGAA+TGG | - | chr7.3:53364736-53364755 | MS.gene063063:CDS | 20.0% |
| ! | GTTGAGATGAGAAATAATAA+GGG | + | chr7.3:53364209-53364228 | None:intergenic | 25.0% |
| ! | TGTTGAGATGAGAAATAATA+AGG | + | chr7.3:53364210-53364229 | None:intergenic | 25.0% |
| !! | AATCCATTCCAAAAACATTT+TGG | - | chr7.3:53364146-53364165 | MS.gene063063:CDS | 25.0% |
| !! | AGGATTAACCAAAATGTTTT+TGG | + | chr7.3:53364157-53364176 | None:intergenic | 25.0% |
| !!! | TAACCAAAATGTTTTTGGAA+TGG | + | chr7.3:53364152-53364171 | None:intergenic | 25.0% |
| ATATGAGTGTACTCAATTTG+TGG | + | chr7.3:53364357-53364376 | None:intergenic | 30.0% | |
| TTATGATAGCTACTAAAGAG+AGG | + | chr7.3:53364177-53364196 | None:intergenic | 30.0% | |
| !!! | CTGTGAGTCAATGTTATTTT+TGG | + | chr7.3:53364542-53364561 | None:intergenic | 30.0% |
| !!! | GTTTTTGGAATGGATTTTCT+TGG | + | chr7.3:53364142-53364161 | None:intergenic | 30.0% |
| ATTTGTCCATGGATAGAGTT+TGG | - | chr7.3:53364497-53364516 | MS.gene063063:CDS | 35.0% | |
| CTTAACTCATGTATCAGAAG+TGG | - | chr7.3:53364300-53364319 | MS.gene063063:CDS | 35.0% | |
| GAATGACTGTGAGGAATTAA+TGG | - | chr7.3:53364474-53364493 | MS.gene063063:CDS | 35.0% | |
| TGTACTCAATTTGTGGTCTT+TGG | + | chr7.3:53364350-53364369 | None:intergenic | 35.0% | |
| TTTGTCCATGGATAGAGTTT+GGG | - | chr7.3:53364498-53364517 | MS.gene063063:CDS | 35.0% | |
| ! | AAATGCTTTGAATGACTGTG+AGG | - | chr7.3:53364465-53364484 | MS.gene063063:CDS | 35.0% |
| !! | GGAATTAATGGATTTGTCCA+TGG | - | chr7.3:53364486-53364505 | MS.gene063063:CDS | 35.0% |
| !! | TCAAAGCATTTTCCTCTCTA+GGG | + | chr7.3:53364456-53364475 | None:intergenic | 35.0% |
| !! | TTCAAAGCATTTTCCTCTCT+AGG | + | chr7.3:53364457-53364476 | None:intergenic | 35.0% |
| AGAGCATGCTCACATAGATT+AGG | + | chr7.3:53364265-53364284 | None:intergenic | 40.0% | |
| ATCGACTGAACACATTCGAA+AGG | - | chr7.3:53364390-53364409 | MS.gene063063:CDS | 40.0% | |
| ATCTTCTGTAGGGGACATAA+AGG | - | chr7.3:53364804-53364823 | MS.gene063063:CDS | 40.0% | |
| CATGTATCAGAAGTGGTTCA+AGG | - | chr7.3:53364307-53364326 | MS.gene063063:CDS | 40.0% | |
| GAGAGATTGAAGAAGGAGAT+AGG | + | chr7.3:53364235-53364254 | None:intergenic | 40.0% | |
| GCTTGTCTAGCAAAGATTGT+TGG | + | chr7.3:53364120-53364139 | None:intergenic | 40.0% | |
| TCCATCTTGGATGACAAGTA+AGG | - | chr7.3:53364765-53364784 | MS.gene063063:CDS | 40.0% | |
| TCCTTACTTGTCATCCAAGA+TGG | + | chr7.3:53364769-53364788 | None:intergenic | 40.0% | |
| TCGAATGTGTTCAGTCGATT+TGG | + | chr7.3:53364389-53364408 | None:intergenic | 40.0% | |
| TGAACACATTCGAAAGGTCA+TGG | - | chr7.3:53364396-53364415 | MS.gene063063:CDS | 40.0% | |
| TTTGGTTAGAGTCAACACTG+AGG | + | chr7.3:53364524-53364543 | None:intergenic | 40.0% | |
| ! | AAGTAAGGATCGAAGGCTTT+TGG | - | chr7.3:53364780-53364799 | MS.gene063063:CDS | 40.0% |
| ! | TGAATGGCGATTTTCCATCT+TGG | - | chr7.3:53364752-53364771 | MS.gene063063:CDS | 40.0% |
| !! | AACTTGCTTGGATGGATTAG+AGG | - | chr7.3:53364606-53364625 | MS.gene063063:CDS | 40.0% |
| !! | ACTTGCTTGGATGGATTAGA+GGG | - | chr7.3:53364607-53364626 | MS.gene063063:CDS | 40.0% |
| !! | ATGGATTAGAGGGTTCATCT+CGG | - | chr7.3:53364617-53364636 | MS.gene063063:CDS | 40.0% |
| !!! | AGGCTTTTGGAATCTTCTGT+AGG | - | chr7.3:53364793-53364812 | MS.gene063063:CDS | 40.0% |
| !!! | GCTTTTGGAATCTTCTGTAG+GGG | - | chr7.3:53364795-53364814 | MS.gene063063:CDS | 40.0% |
| !!! | GGCTTTTGGAATCTTCTGTA+GGG | - | chr7.3:53364794-53364813 | MS.gene063063:CDS | 40.0% |
| AGCTGAGGAGAGATTGAAGA+AGG | + | chr7.3:53364242-53364261 | None:intergenic | 45.0% | |
| ATCCATCCAAGCAAGTTGCA+TGG | + | chr7.3:53364603-53364622 | None:intergenic | 45.0% | |
| CACAGTATGATGCACACACA+TGG | - | chr7.3:53364557-53364576 | MS.gene063063:CDS | 45.0% | |
| CATAAAGGCCAATGTTGTGG+TGG | - | chr7.3:53364819-53364838 | MS.gene063063:CDS | 45.0% | |
| CATTCGAAAGGTCATGGACA+CGG | - | chr7.3:53364402-53364421 | MS.gene063063:CDS | 45.0% | |
| CGTTATGGAGAGTAACCTTC+AGG | - | chr7.3:53364642-53364661 | MS.gene063063:CDS | 45.0% | |
| CTCACATAGATTAGGAGCTG+AGG | + | chr7.3:53364257-53364276 | None:intergenic | 45.0% | |
| GGACATAAAGGCCAATGTTG+TGG | - | chr7.3:53364816-53364835 | MS.gene063063:CDS | 45.0% | |
| GGATGACAAGTAAGGATCGA+AGG | - | chr7.3:53364773-53364792 | MS.gene063063:CDS | 45.0% | |
| ! | AACCATGCAACTTGCTTGGA+TGG | - | chr7.3:53364598-53364617 | MS.gene063063:CDS | 45.0% |
| ! | CACTAACCATGCAACTTGCT+TGG | - | chr7.3:53364594-53364613 | MS.gene063063:CDS | 45.0% |
| ! | TGGATTAGAGGGTTCATCTC+GGG | - | chr7.3:53364618-53364637 | MS.gene063063:CDS | 45.0% |
| ! | TGTTTCTGTCTTGCCTCCAA+AGG | - | chr7.3:53364702-53364721 | MS.gene063063:CDS | 45.0% |
| !! | ATGAATCCGTCGTTAGCCTT+TGG | + | chr7.3:53364721-53364740 | None:intergenic | 45.0% |
| !! | CCTCTCTAGGGCTATTAACA+CGG | + | chr7.3:53364444-53364463 | None:intergenic | 45.0% |
| AATGTTGTGGTGGCTCAGGA+CGG | - | chr7.3:53364829-53364848 | MS.gene063063:CDS | 50.0% | |
| ACCTTCAGGACTTGATCTCC+AGG | - | chr7.3:53364656-53364675 | MS.gene063063:CDS | 50.0% | |
| AGCAAGAGAAGATCTAGCCC+TGG | + | chr7.3:53364677-53364696 | None:intergenic | 50.0% | |
| CCGTGTTAATAGCCCTAGAG+AGG | - | chr7.3:53364441-53364460 | MS.gene063063:CDS | 50.0% | |
| CCTTCAGGACTTGATCTCCA+GGG | - | chr7.3:53364657-53364676 | MS.gene063063:CDS | 50.0% | |
| TGAGGCCCAAACTCTATCCA+TGG | + | chr7.3:53364506-53364525 | None:intergenic | 50.0% | |
| TTGCCTCCAAAGGCTAACGA+CGG | - | chr7.3:53364712-53364731 | MS.gene063063:CDS | 50.0% | |
| !! | AATCCGTCGTTAGCCTTTGG+AGG | + | chr7.3:53364718-53364737 | None:intergenic | 50.0% |
| ATGTTGTGGTGGCTCAGGAC+GGG | - | chr7.3:53364830-53364849 | MS.gene063063:CDS | 55.0% | |
| CCCTGGAGATCAAGTCCTGA+AGG | + | chr7.3:53364660-53364679 | None:intergenic | 55.0% | |
| GAGCGGCAAGTTCAAGACTG+TGG | - | chr7.3:53364852-53364871 | MS.gene063063:CDS | 55.0% | |
| GGGTTCATCTCGGGTCGTTA+TGG | - | chr7.3:53364627-53364646 | MS.gene063063:CDS | 55.0% | |
| GTCCTGAGCCACCACAACAT+TGG | + | chr7.3:53364830-53364849 | None:intergenic | 55.0% | |
| GGCCAATGTTGTGGTGGCTC+AGG | - | chr7.3:53364825-53364844 | MS.gene063063:CDS | 60.0% | |
| GTGGTGGCTCAGGACGGGAG+CGG | - | chr7.3:53364835-53364854 | MS.gene063063:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 53364109 | 53364904 | 53364109 | ID=MS.gene063063 |
| chr7.3 | mRNA | 53364109 | 53364904 | 53364109 | ID=MS.gene063063.t1;Parent=MS.gene063063 |
| chr7.3 | exon | 53364109 | 53364904 | 53364109 | ID=MS.gene063063.t1.exon1;Parent=MS.gene063063.t1 |
| chr7.3 | CDS | 53364109 | 53364904 | 53364109 | ID=cds.MS.gene063063.t1;Parent=MS.gene063063.t1 |
| Gene Sequence |
| Protein sequence |
