Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94266.t1 | XP_003623195.1 | 95.1 | 224 | 11 | 0 | 1 | 224 | 1 | 224 | 3.60E-119 | 437.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94266.t1 | P32110 | 73.3 | 225 | 59 | 1 | 1 | 224 | 1 | 225 | 3.9e-90 | 332.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94266.t1 | G7L6G9 | 95.1 | 224 | 11 | 0 | 1 | 224 | 1 | 224 | 2.6e-119 | 437.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049181 | MS.gene94266 | 0.804034 | 2.62E-49 | -1.69E-46 |
| MS.gene049182 | MS.gene94266 | 0.818367 | 2.03E-52 | -1.69E-46 |
| MS.gene04920 | MS.gene94266 | 0.808161 | 3.55E-50 | -1.69E-46 |
| MS.gene049283 | MS.gene94266 | 0.830418 | 2.97E-55 | -1.69E-46 |
| MS.gene049499 | MS.gene94266 | 0.801805 | 7.57E-49 | -1.69E-46 |
| MS.gene049680 | MS.gene94266 | 0.827206 | 1.78E-54 | -1.69E-46 |
| MS.gene050379 | MS.gene94266 | 0.823317 | 1.48E-53 | -1.69E-46 |
| MS.gene050945 | MS.gene94266 | 0.818529 | 1.87E-52 | -1.69E-46 |
| MS.gene05156 | MS.gene94266 | 0.817796 | 2.74E-52 | -1.69E-46 |
| MS.gene051637 | MS.gene94266 | 0.801942 | 7.10E-49 | -1.69E-46 |
| MS.gene051699 | MS.gene94266 | 0.816777 | 4.64E-52 | -1.69E-46 |
| MS.gene051959 | MS.gene94266 | 0.840072 | 1.08E-57 | -1.69E-46 |
| MS.gene051960 | MS.gene94266 | 0.838841 | 2.26E-57 | -1.69E-46 |
| MS.gene052040 | MS.gene94266 | 0.826429 | 2.73E-54 | -1.69E-46 |
| MS.gene052053 | MS.gene94266 | 0.849116 | 3.98E-60 | -1.69E-46 |
| MS.gene052533 | MS.gene94266 | 0.810179 | 1.31E-50 | -1.69E-46 |
| MS.gene052579 | MS.gene94266 | 0.807665 | 4.52E-50 | -1.69E-46 |
| MS.gene053602 | MS.gene94266 | 0.845425 | 4.10E-59 | -1.69E-46 |
| MS.gene053963 | MS.gene94266 | 0.80919 | 2.14E-50 | -1.69E-46 |
| MS.gene054413 | MS.gene94266 | 0.817791 | 2.74E-52 | -1.69E-46 |
| MS.gene054892 | MS.gene94266 | 0.810868 | 9.31E-51 | -1.69E-46 |
| MS.gene054893 | MS.gene94266 | 0.812749 | 3.63E-51 | -1.69E-46 |
| MS.gene054957 | MS.gene94266 | 0.840428 | 8.75E-58 | -1.69E-46 |
| MS.gene054960 | MS.gene94266 | 0.809826 | 1.56E-50 | -1.69E-46 |
| MS.gene055147 | MS.gene94266 | 0.814956 | 1.18E-51 | -1.69E-46 |
| MS.gene055148 | MS.gene94266 | 0.81816 | 2.27E-52 | -1.69E-46 |
| MS.gene055150 | MS.gene94266 | 0.927803 | 7.06E-92 | -1.69E-46 |
| MS.gene055151 | MS.gene94266 | 0.834767 | 2.48E-56 | -1.69E-46 |
| MS.gene055155 | MS.gene94266 | 0.822641 | 2.12E-53 | -1.69E-46 |
| MS.gene056100 | MS.gene94266 | 0.816197 | 6.26E-52 | -1.69E-46 |
| MS.gene05624 | MS.gene94266 | 0.836964 | 6.87E-57 | -1.69E-46 |
| MS.gene056530 | MS.gene94266 | 0.849413 | 3.29E-60 | -1.69E-46 |
| MS.gene05667 | MS.gene94266 | 0.811072 | 8.40E-51 | -1.69E-46 |
| MS.gene05686 | MS.gene94266 | 0.802501 | 5.45E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene94266 | 0.8186 | 1.80E-52 | -1.69E-46 |
| MS.gene057148 | MS.gene94266 | -0.83361 | 4.83E-56 | -1.69E-46 |
| MS.gene057472 | MS.gene94266 | 0.861135 | 1.27E-63 | -1.69E-46 |
| MS.gene057622 | MS.gene94266 | 0.82842 | 9.08E-55 | -1.69E-46 |
| MS.gene05808 | MS.gene94266 | 0.805057 | 1.60E-49 | -1.69E-46 |
| MS.gene058095 | MS.gene94266 | 0.808277 | 3.35E-50 | -1.69E-46 |
| MS.gene058592 | MS.gene94266 | 0.837649 | 4.59E-57 | -1.69E-46 |
| MS.gene058781 | MS.gene94266 | 0.815754 | 7.86E-52 | -1.69E-46 |
| MS.gene05878 | MS.gene94266 | 0.855915 | 4.58E-62 | -1.69E-46 |
| MS.gene059436 | MS.gene94266 | 0.83314 | 6.33E-56 | -1.69E-46 |
| MS.gene05952 | MS.gene94266 | 0.872642 | 2.72E-67 | -1.69E-46 |
| MS.gene05956 | MS.gene94266 | 0.827473 | 1.53E-54 | -1.69E-46 |
| MS.gene05985 | MS.gene94266 | -0.868677 | 5.47E-66 | -1.69E-46 |
| MS.gene059867 | MS.gene94266 | 0.803923 | 2.77E-49 | -1.69E-46 |
| MS.gene059901 | MS.gene94266 | -0.824963 | 6.07E-54 | -1.69E-46 |
| MS.gene060237 | MS.gene94266 | 0.802007 | 6.88E-49 | -1.69E-46 |
| MS.gene06023 | MS.gene94266 | 0.833281 | 5.84E-56 | -1.69E-46 |
| MS.gene060244 | MS.gene94266 | 0.817037 | 4.06E-52 | -1.69E-46 |
| MS.gene060338 | MS.gene94266 | 0.837166 | 6.10E-57 | -1.69E-46 |
| MS.gene060522 | MS.gene94266 | 0.809957 | 1.46E-50 | -1.69E-46 |
| MS.gene060935 | MS.gene94266 | 0.81933 | 1.23E-52 | -1.69E-46 |
| MS.gene060937 | MS.gene94266 | 0.830596 | 2.69E-55 | -1.69E-46 |
| MS.gene060975 | MS.gene94266 | 0.834083 | 3.68E-56 | -1.69E-46 |
| MS.gene060977 | MS.gene94266 | 0.843244 | 1.58E-58 | -1.69E-46 |
| MS.gene061188 | MS.gene94266 | 0.801131 | 1.04E-48 | -1.69E-46 |
| MS.gene061243 | MS.gene94266 | 0.809167 | 2.16E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94266.t1 | MTR_7g065590 | 95.089 | 224 | 11 | 0 | 1 | 224 | 1 | 224 | 9.88e-158 | 436 |
| MS.gene94266.t1 | MTR_7g065600 | 81.250 | 224 | 42 | 0 | 1 | 224 | 1 | 224 | 6.82e-135 | 378 |
| MS.gene94266.t1 | MTR_7g065290 | 80.357 | 224 | 44 | 0 | 1 | 224 | 1 | 224 | 1.34e-129 | 364 |
| MS.gene94266.t1 | MTR_7g065230 | 79.464 | 224 | 41 | 1 | 1 | 224 | 1 | 219 | 3.75e-125 | 353 |
| MS.gene94266.t1 | MTR_7g065265 | 78.571 | 224 | 48 | 0 | 1 | 224 | 1 | 224 | 9.01e-125 | 352 |
| MS.gene94266.t1 | MTR_7g065270 | 77.232 | 224 | 51 | 0 | 1 | 224 | 1 | 224 | 2.73e-124 | 351 |
| MS.gene94266.t1 | MTR_7g065260 | 78.667 | 225 | 47 | 1 | 1 | 224 | 8 | 232 | 2.20e-116 | 331 |
| MS.gene94266.t1 | MTR_7g065660 | 69.058 | 223 | 69 | 0 | 1 | 223 | 1 | 223 | 1.81e-114 | 326 |
| MS.gene94266.t1 | MTR_7g065720 | 69.778 | 225 | 67 | 1 | 1 | 224 | 1 | 225 | 5.24e-114 | 325 |
| MS.gene94266.t1 | MTR_7g065630 | 70.404 | 223 | 62 | 1 | 1 | 223 | 19 | 237 | 1.77e-113 | 324 |
| MS.gene94266.t1 | MTR_7g065740 | 65.179 | 224 | 77 | 1 | 1 | 223 | 1 | 224 | 4.07e-109 | 313 |
| MS.gene94266.t1 | MTR_7g065750 | 66.964 | 224 | 73 | 1 | 1 | 223 | 1 | 224 | 4.07e-109 | 313 |
| MS.gene94266.t1 | MTR_4g019790 | 62.780 | 223 | 83 | 0 | 1 | 223 | 1 | 223 | 5.05e-105 | 302 |
| MS.gene94266.t1 | MTR_7g065640 | 64.574 | 223 | 61 | 1 | 1 | 223 | 1 | 205 | 5.24e-102 | 294 |
| MS.gene94266.t1 | MTR_7g065680 | 67.568 | 222 | 68 | 1 | 1 | 222 | 1 | 218 | 3.40e-98 | 285 |
| MS.gene94266.t1 | MTR_7g065710 | 66.667 | 207 | 65 | 1 | 8 | 214 | 5 | 207 | 1.96e-97 | 283 |
| MS.gene94266.t1 | MTR_4g019780 | 63.084 | 214 | 77 | 2 | 3 | 215 | 4 | 216 | 4.62e-96 | 280 |
| MS.gene94266.t1 | MTR_7g065700 | 67.290 | 214 | 66 | 1 | 1 | 214 | 1 | 210 | 1.33e-93 | 273 |
| MS.gene94266.t1 | MTR_3g099757 | 48.182 | 220 | 112 | 2 | 5 | 223 | 3 | 221 | 1.76e-65 | 202 |
| MS.gene94266.t1 | MTR_6g080440 | 47.596 | 208 | 107 | 2 | 7 | 213 | 3 | 209 | 2.31e-65 | 202 |
| MS.gene94266.t1 | MTR_1g090150 | 46.606 | 221 | 113 | 3 | 5 | 222 | 1 | 219 | 6.50e-64 | 198 |
| MS.gene94266.t1 | MTR_8g087410 | 47.465 | 217 | 111 | 3 | 1 | 216 | 1 | 215 | 1.20e-62 | 195 |
| MS.gene94266.t1 | MTR_8g087425 | 45.161 | 217 | 117 | 2 | 4 | 219 | 2 | 217 | 4.52e-61 | 191 |
| MS.gene94266.t1 | MTR_1g115195 | 44.545 | 220 | 115 | 3 | 7 | 221 | 3 | 220 | 8.19e-60 | 187 |
| MS.gene94266.t1 | MTR_5g040430 | 42.793 | 222 | 119 | 3 | 1 | 215 | 1 | 221 | 1.55e-56 | 179 |
| MS.gene94266.t1 | MTR_1g090070 | 42.534 | 221 | 122 | 3 | 7 | 223 | 9 | 228 | 1.64e-56 | 179 |
| MS.gene94266.t1 | MTR_4g059730 | 42.056 | 214 | 118 | 3 | 6 | 215 | 3 | 214 | 8.22e-56 | 177 |
| MS.gene94266.t1 | MTR_1g090060 | 42.857 | 224 | 118 | 5 | 7 | 224 | 3 | 222 | 1.79e-55 | 176 |
| MS.gene94266.t1 | MTR_1g090100 | 41.818 | 220 | 122 | 3 | 8 | 223 | 3 | 220 | 2.69e-53 | 171 |
| MS.gene94266.t1 | MTR_1g090090 | 42.381 | 210 | 115 | 3 | 7 | 212 | 3 | 210 | 5.14e-52 | 167 |
| MS.gene94266.t1 | MTR_8g061950 | 44.292 | 219 | 114 | 5 | 5 | 215 | 3 | 221 | 2.37e-50 | 164 |
| MS.gene94266.t1 | MTR_5g037380 | 42.273 | 220 | 117 | 4 | 1 | 212 | 1 | 218 | 6.08e-49 | 160 |
| MS.gene94266.t1 | MTR_2g070150 | 38.235 | 204 | 121 | 4 | 6 | 207 | 3 | 203 | 6.48e-43 | 144 |
| MS.gene94266.t1 | MTR_3g467420 | 42.211 | 199 | 110 | 5 | 16 | 212 | 18 | 213 | 3.54e-42 | 142 |
| MS.gene94266.t1 | MTR_2g070200 | 38.863 | 211 | 124 | 4 | 6 | 213 | 3 | 211 | 3.95e-42 | 142 |
| MS.gene94266.t1 | MTR_2g070130 | 38.350 | 206 | 122 | 4 | 6 | 209 | 3 | 205 | 4.30e-42 | 142 |
| MS.gene94266.t1 | MTR_4g124130 | 45.263 | 190 | 98 | 5 | 5 | 191 | 2 | 188 | 1.78e-41 | 140 |
| MS.gene94266.t1 | MTR_2g070140 | 37.949 | 195 | 116 | 4 | 21 | 213 | 2 | 193 | 7.63e-41 | 138 |
| MS.gene94266.t1 | MTR_2g070120 | 39.906 | 213 | 117 | 7 | 6 | 213 | 3 | 209 | 2.53e-40 | 137 |
| MS.gene94266.t1 | MTR_2g070180 | 36.765 | 204 | 124 | 4 | 6 | 207 | 3 | 203 | 4.94e-40 | 137 |
| MS.gene94266.t1 | MTR_3g467430 | 38.863 | 211 | 124 | 5 | 4 | 212 | 6 | 213 | 7.25e-40 | 137 |
| MS.gene94266.t1 | MTR_2g070210 | 38.679 | 212 | 123 | 5 | 6 | 213 | 3 | 211 | 8.03e-40 | 136 |
| MS.gene94266.t1 | MTR_8g056940 | 38.356 | 219 | 125 | 6 | 6 | 218 | 3 | 217 | 8.30e-39 | 134 |
| MS.gene94266.t1 | MTR_2g070060 | 37.500 | 208 | 125 | 4 | 7 | 212 | 4 | 208 | 1.69e-37 | 130 |
| MS.gene94266.t1 | MTR_2g070070 | 37.500 | 216 | 130 | 4 | 7 | 220 | 4 | 216 | 3.65e-37 | 129 |
| MS.gene94266.t1 | MTR_5g076900 | 36.321 | 212 | 130 | 4 | 3 | 212 | 2 | 210 | 1.88e-35 | 125 |
| MS.gene94266.t1 | MTR_2g070110 | 39.500 | 200 | 115 | 5 | 7 | 204 | 4 | 199 | 3.00e-32 | 117 |
| MS.gene94266.t1 | MTR_0186s0030 | 54.255 | 94 | 42 | 1 | 16 | 108 | 13 | 106 | 1.46e-31 | 112 |
| MS.gene94266.t1 | MTR_2g072120 | 45.714 | 70 | 36 | 2 | 51 | 119 | 2 | 70 | 6.48e-16 | 70.5 |
| MS.gene94266.t1 | MTR_4g134370 | 30.000 | 230 | 129 | 9 | 1 | 207 | 1 | 221 | 1.52e-12 | 65.1 |
| MS.gene94266.t1 | MTR_1g067180 | 26.667 | 225 | 131 | 8 | 8 | 217 | 25 | 230 | 9.67e-12 | 62.8 |
| MS.gene94266.t1 | MTR_1g067180 | 26.667 | 225 | 131 | 8 | 8 | 217 | 28 | 233 | 2.36e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94266.t1 | AT3G09270 | 53.571 | 224 | 99 | 5 | 4 | 223 | 2 | 224 | 1.08e-75 | 228 |
| MS.gene94266.t1 | AT2G29420 | 49.772 | 219 | 108 | 2 | 3 | 220 | 5 | 222 | 2.84e-75 | 227 |
| MS.gene94266.t1 | AT2G29450 | 49.275 | 207 | 103 | 2 | 3 | 207 | 2 | 208 | 2.15e-60 | 189 |
| MS.gene94266.t1 | AT2G29490 | 48.325 | 209 | 105 | 3 | 1 | 207 | 1 | 208 | 4.40e-60 | 188 |
| MS.gene94266.t1 | AT2G29480 | 47.847 | 209 | 106 | 3 | 1 | 207 | 1 | 208 | 1.73e-58 | 184 |
| MS.gene94266.t1 | AT2G29460 | 45.778 | 225 | 119 | 3 | 1 | 223 | 1 | 224 | 4.87e-58 | 183 |
| MS.gene94266.t1 | AT2G29470 | 46.190 | 210 | 109 | 4 | 1 | 207 | 1 | 209 | 1.59e-56 | 179 |
| MS.gene94266.t1 | AT2G29440 | 46.411 | 209 | 108 | 4 | 1 | 207 | 1 | 207 | 1.67e-55 | 177 |
| MS.gene94266.t1 | AT1G69930 | 38.938 | 226 | 132 | 3 | 2 | 221 | 7 | 232 | 7.46e-50 | 162 |
| MS.gene94266.t1 | AT1G59700 | 44.037 | 218 | 114 | 4 | 5 | 214 | 4 | 221 | 2.60e-48 | 159 |
| MS.gene94266.t1 | AT1G10370 | 41.176 | 221 | 121 | 4 | 1 | 214 | 1 | 219 | 8.77e-48 | 157 |
| MS.gene94266.t1 | AT1G59670 | 42.202 | 218 | 118 | 4 | 5 | 214 | 4 | 221 | 3.98e-47 | 155 |
| MS.gene94266.t1 | AT1G78320 | 40.865 | 208 | 118 | 4 | 5 | 210 | 2 | 206 | 7.03e-47 | 154 |
| MS.gene94266.t1 | AT5G62480 | 40.271 | 221 | 121 | 4 | 5 | 215 | 6 | 225 | 8.97e-47 | 155 |
| MS.gene94266.t1 | AT1G74590 | 39.091 | 220 | 127 | 3 | 1 | 214 | 1 | 219 | 1.48e-46 | 154 |
| MS.gene94266.t1 | AT1G27130 | 37.333 | 225 | 132 | 4 | 1 | 217 | 1 | 224 | 1.05e-45 | 152 |
| MS.gene94266.t1 | AT1G78340 | 42.105 | 209 | 116 | 4 | 6 | 212 | 3 | 208 | 1.22e-45 | 151 |
| MS.gene94266.t1 | AT1G78380 | 39.904 | 208 | 120 | 4 | 7 | 212 | 4 | 208 | 6.62e-44 | 147 |
| MS.gene94266.t1 | AT1G78370 | 40.201 | 199 | 114 | 4 | 16 | 212 | 13 | 208 | 1.08e-43 | 146 |
| MS.gene94266.t1 | AT1G10360 | 37.037 | 216 | 129 | 3 | 6 | 214 | 4 | 219 | 1.62e-43 | 146 |
| MS.gene94266.t1 | AT1G17170 | 38.710 | 217 | 127 | 5 | 6 | 220 | 3 | 215 | 2.23e-43 | 145 |
| MS.gene94266.t1 | AT1G27140 | 36.564 | 227 | 134 | 4 | 1 | 218 | 1 | 226 | 4.12e-43 | 145 |
| MS.gene94266.t1 | AT1G69920 | 37.118 | 229 | 130 | 5 | 1 | 217 | 26 | 252 | 2.47e-42 | 144 |
| MS.gene94266.t1 | AT1G17190 | 42.487 | 193 | 104 | 5 | 1 | 191 | 1 | 188 | 3.48e-41 | 140 |
| MS.gene94266.t1 | AT1G78360 | 40.191 | 209 | 119 | 5 | 7 | 212 | 4 | 209 | 1.03e-39 | 136 |
| MS.gene94266.t1 | AT1G17180 | 37.799 | 209 | 125 | 4 | 6 | 212 | 3 | 208 | 2.17e-39 | 135 |
| MS.gene94266.t1 | AT3G43800 | 37.198 | 207 | 121 | 6 | 4 | 204 | 2 | 205 | 2.01e-38 | 133 |
| MS.gene94266.t1 | AT1G53680 | 37.143 | 210 | 127 | 4 | 1 | 207 | 1 | 208 | 3.61e-38 | 132 |
| MS.gene94266.t1 | AT1G78320 | 32.850 | 207 | 109 | 4 | 5 | 210 | 2 | 179 | 9.25e-29 | 107 |
| MS.gene94266.t1 | AT5G62480 | 31.364 | 220 | 115 | 4 | 5 | 215 | 6 | 198 | 1.01e-27 | 105 |
| MS.gene94266.t1 | AT5G62480 | 31.364 | 220 | 116 | 4 | 5 | 215 | 6 | 199 | 1.04e-27 | 105 |
| MS.gene94266.t1 | AT2G02380 | 28.704 | 216 | 133 | 7 | 5 | 207 | 9 | 216 | 1.45e-14 | 70.5 |
| MS.gene94266.t1 | AT2G02390 | 28.444 | 225 | 133 | 7 | 3 | 207 | 4 | 220 | 2.18e-13 | 67.4 |
| MS.gene94266.t1 | AT2G02390 | 29.358 | 218 | 133 | 7 | 3 | 207 | 4 | 213 | 3.51e-13 | 66.6 |
Find 38 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAGCCTTGGCTCGTTTC+TGG | 0.160272 | 7.3:+40589673 | MS.gene94266:CDS |
| ATTTCAACCCTTGTAGAAAA+TGG | 0.235130 | 7.3:-40589444 | None:intergenic |
| ACAGGAACTTTCTTATAAAC+AGG | 0.283283 | 7.3:-40589549 | None:intergenic |
| CCTGTTACTTCTTGTACAAT+AGG | 0.286054 | 7.3:-40590186 | None:intergenic |
| TTGGGTGGTTGTTGAATTCT+TGG | 0.290435 | 7.3:-40590257 | None:intergenic |
| GAGCTCAAGGGCAAGTTCTT+TGG | 0.302605 | 7.3:+40590108 | MS.gene94266:CDS |
| GCCTCGTAGAGAAGGGCTTT+TGG | 0.332731 | 7.3:+40590296 | MS.gene94266:CDS |
| TAAGGATCAGAAGGCAAGAT+TGG | 0.379369 | 7.3:-40589648 | None:intergenic |
| GTTCCTGTATTAGTTCATAA+TGG | 0.400419 | 7.3:+40589564 | MS.gene94266:CDS |
| CACTAAGATGGCATCAAATC+AGG | 0.403482 | 7.3:+40589389 | None:intergenic |
| TTCTTTGGTGGAGATGAGAT+TGG | 0.406091 | 7.3:+40590123 | MS.gene94266:CDS |
| CCTATTGTACAAGAAGTAAC+AGG | 0.429315 | 7.3:+40590186 | MS.gene94266:CDS |
| GTCCAAGTTCATAGATGATA+AGG | 0.434027 | 7.3:+40589695 | MS.gene94266:CDS |
| CTGTTACTTCTTGTACAATA+GGG | 0.437463 | 7.3:-40590185 | None:intergenic |
| TGGCTCCAATTGTAAAGCTT+AGG | 0.444725 | 7.3:-40590237 | None:intergenic |
| AATTTCCTAAGCTTTACAAT+TGG | 0.445365 | 7.3:+40590232 | MS.gene94266:CDS |
| TGAAATAGCTCTAAAATTGA+AGG | 0.446842 | 7.3:+40589461 | MS.gene94266:CDS |
| CAAATATGAGGAAGAAAAGC+AGG | 0.457444 | 7.3:+40589494 | MS.gene94266:CDS |
| TACCTTATCATCTATGAACT+TGG | 0.459673 | 7.3:-40589697 | None:intergenic |
| ATCACAAGTGACTCTGATAT+AGG | 0.471671 | 7.3:-40589591 | None:intergenic |
| GAAAATTTGCCTCGTAGAGA+AGG | 0.475442 | 7.3:+40590288 | MS.gene94266:CDS |
| TTGCCATTATGAACTAATAC+AGG | 0.495358 | 7.3:-40589567 | None:intergenic |
| CTCAAGGGCAAGTTCTTTGG+TGG | 0.497594 | 7.3:+40590111 | MS.gene94266:CDS |
| TTCATAACGAGCTTTGAAGA+AGG | 0.517849 | 7.3:-40590319 | None:intergenic |
| GGGTGTTGAATACAAATATG+AGG | 0.518356 | 7.3:+40589482 | MS.gene94266:CDS |
| TTGAATACATCGATGAGACT+TGG | 0.524005 | 7.3:+40589616 | MS.gene94266:CDS |
| TGATCCTTATAAAAGAGCCT+TGG | 0.539754 | 7.3:+40589662 | MS.gene94266:CDS |
| AAAATTTGCCTCGTAGAGAA+GGG | 0.551755 | 7.3:+40590289 | MS.gene94266:CDS |
| ATTTCTTGAGAATGAGCTCA+AGG | 0.554660 | 7.3:+40590095 | MS.gene94266:CDS |
| CAGGAACTTTCTTATAAACA+GGG | 0.564477 | 7.3:-40589548 | None:intergenic |
| GAAATAGCTCTAAAATTGAA+GGG | 0.600047 | 7.3:+40589462 | MS.gene94266:CDS |
| CAACAACCACCCAATTGTCA+AGG | 0.606941 | 7.3:+40590266 | MS.gene94266:CDS |
| TTTCTTGAGAATGAGCTCAA+GGG | 0.611348 | 7.3:+40590096 | MS.gene94266:CDS |
| AAATATGAGGAAGAAAAGCA+GGG | 0.615123 | 7.3:+40589495 | MS.gene94266:CDS |
| GCCAAAAGCCCTTCTCTACG+AGG | 0.649932 | 7.3:-40590297 | None:intergenic |
| ACCAGTGTATGATTCCTGCA+TGG | 0.670185 | 7.3:+40590004 | MS.gene94266:intron |
| CTTGGACCAGAAACGAGCCA+AGG | 0.694386 | 7.3:-40589679 | None:intergenic |
| AATATGAGGAAGAAAAGCAG+GGG | 0.696106 | 7.3:+40589496 | MS.gene94266:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATCATATTTGAGAAATAT+TGG | - | chr7.3:40589891-40589910 | None:intergenic | 15.0% |
| !! | TTAAGGTCATTATAAAAATT+AGG | - | chr7.3:40589976-40589995 | None:intergenic | 15.0% |
| !!! | TTGAATATTGATTAGAAAAA+GGG | + | chr7.3:40589864-40589883 | MS.gene94266:intron | 15.0% |
| !!! | TTTGAATATTGATTAGAAAA+AGG | + | chr7.3:40589863-40589882 | MS.gene94266:intron | 15.0% |
| ! | AATTTCCTAAGCTTTACAAT+TGG | + | chr7.3:40590232-40590251 | MS.gene94266:CDS | 25.0% |
| ! | ACACACATATATAATGAACT+TGG | - | chr7.3:40589766-40589785 | None:intergenic | 25.0% |
| ! | CAAATATGATTAAGTTAGCT+GGG | + | chr7.3:40589899-40589918 | MS.gene94266:intron | 25.0% |
| ! | TCAAATATGATTAAGTTAGC+TGG | + | chr7.3:40589898-40589917 | MS.gene94266:intron | 25.0% |
| !!! | GAAATAGCTCTAAAATTGAA+GGG | + | chr7.3:40589462-40589481 | MS.gene94266:CDS | 25.0% |
| !!! | TGAAATAGCTCTAAAATTGA+AGG | + | chr7.3:40589461-40589480 | MS.gene94266:CDS | 25.0% |
| AAATATGAGGAAGAAAAGCA+GGG | + | chr7.3:40589495-40589514 | MS.gene94266:CDS | 30.0% | |
| ACAGGAACTTTCTTATAAAC+AGG | - | chr7.3:40589552-40589571 | None:intergenic | 30.0% | |
| ATTTCAACCCTTGTAGAAAA+TGG | - | chr7.3:40589447-40589466 | None:intergenic | 30.0% | |
| CAGGAACTTTCTTATAAACA+GGG | - | chr7.3:40589551-40589570 | None:intergenic | 30.0% | |
| GTTCCTGTATTAGTTCATAA+TGG | + | chr7.3:40589564-40589583 | MS.gene94266:CDS | 30.0% | |
| TACCTTATCATCTATGAACT+TGG | - | chr7.3:40589700-40589719 | None:intergenic | 30.0% | |
| TTGCCATTATGAACTAATAC+AGG | - | chr7.3:40589570-40589589 | None:intergenic | 30.0% | |
| ! | ATATAATGAACTTGGACTAG+AGG | - | chr7.3:40589758-40589777 | None:intergenic | 30.0% |
| ! | CTGTTACTTCTTGTACAATA+GGG | - | chr7.3:40590188-40590207 | None:intergenic | 30.0% |
| ! | GCAAATTTTCCTTGACAATT+GGG | - | chr7.3:40590278-40590297 | None:intergenic | 30.0% |
| AAAATTTGCCTCGTAGAGAA+GGG | + | chr7.3:40590289-40590308 | MS.gene94266:CDS | 35.0% | |
| AAGAAAAGGGATCAGAAGAT+TGG | - | chr7.3:40589726-40589745 | None:intergenic | 35.0% | |
| AATATGAGGAAGAAAAGCAG+GGG | + | chr7.3:40589496-40589515 | MS.gene94266:CDS | 35.0% | |
| ATCACAAGTGACTCTGATAT+AGG | - | chr7.3:40589594-40589613 | None:intergenic | 35.0% | |
| ATTTCTTGAGAATGAGCTCA+AGG | + | chr7.3:40590095-40590114 | MS.gene94266:CDS | 35.0% | |
| CAAATATGAGGAAGAAAAGC+AGG | + | chr7.3:40589494-40589513 | MS.gene94266:CDS | 35.0% | |
| CCTATTGTACAAGAAGTAAC+AGG | + | chr7.3:40590186-40590205 | MS.gene94266:CDS | 35.0% | |
| GTCCAAGTTCATAGATGATA+AGG | + | chr7.3:40589695-40589714 | MS.gene94266:CDS | 35.0% | |
| TGATCCTTATAAAAGAGCCT+TGG | + | chr7.3:40589662-40589681 | MS.gene94266:CDS | 35.0% | |
| TTGAATACATCGATGAGACT+TGG | + | chr7.3:40589616-40589635 | MS.gene94266:CDS | 35.0% | |
| TTTCTTGAGAATGAGCTCAA+GGG | + | chr7.3:40590096-40590115 | MS.gene94266:CDS | 35.0% | |
| ! | AATTTTCCTTGACAATTGGG+TGG | - | chr7.3:40590275-40590294 | None:intergenic | 35.0% |
| ! | CCTGTTACTTCTTGTACAAT+AGG | - | chr7.3:40590189-40590208 | None:intergenic | 35.0% |
| ! | CTGCTGTCTTTATAGCTTTT+TGG | + | chr7.3:40590160-40590179 | MS.gene94266:CDS | 35.0% |
| ! | GCTCTTTTATAAGGATCAGA+AGG | - | chr7.3:40589660-40589679 | None:intergenic | 35.0% |
| ! | GGCAAATTTTCCTTGACAAT+TGG | - | chr7.3:40590279-40590298 | None:intergenic | 35.0% |
| ! | TACACTGGTCTCACAAATTA+AGG | - | chr7.3:40589993-40590012 | None:intergenic | 35.0% |
| !! | GGGTGTTGAATACAAATATG+AGG | + | chr7.3:40589482-40589501 | MS.gene94266:CDS | 35.0% |
| !! | TTCATAACGAGCTTTGAAGA+AGG | - | chr7.3:40590322-40590341 | None:intergenic | 35.0% |
| !!! | GAGTTTTGAGGAAACAAAAG+AGG | + | chr7.3:40590065-40590084 | MS.gene94266:CDS | 35.0% |
| GAAAATTTGCCTCGTAGAGA+AGG | + | chr7.3:40590288-40590307 | MS.gene94266:CDS | 40.0% | |
| TAAGGATCAGAAGGCAAGAT+TGG | - | chr7.3:40589651-40589670 | None:intergenic | 40.0% | |
| TGGCTCCAATTGTAAAGCTT+AGG | - | chr7.3:40590240-40590259 | None:intergenic | 40.0% | |
| TTCTTTGGTGGAGATGAGAT+TGG | + | chr7.3:40590123-40590142 | MS.gene94266:CDS | 40.0% | |
| TTGGGTGGTTGTTGAATTCT+TGG | - | chr7.3:40590260-40590279 | None:intergenic | 40.0% | |
| ! | AGAACGTGAGAAGAGTTTTG+AGG | + | chr7.3:40590053-40590072 | MS.gene94266:CDS | 40.0% |
| ! | CAGGAAGAAGTGAAGCTTTT+CGG | + | chr7.3:40589408-40589427 | MS.gene94266:CDS | 40.0% |
| ! | GGGAAGTCCATTTTCTACAA+GGG | + | chr7.3:40589437-40589456 | MS.gene94266:CDS | 40.0% |
| ! | TGGGAAGTCCATTTTCTACA+AGG | + | chr7.3:40589436-40589455 | MS.gene94266:CDS | 40.0% |
| ACCAGTGTATGATTCCTGCA+TGG | + | chr7.3:40590004-40590023 | MS.gene94266:intron | 45.0% | |
| AGAGGTCACACAGCAAGAAA+AGG | - | chr7.3:40589740-40589759 | None:intergenic | 45.0% | |
| CAACAACCACCCAATTGTCA+AGG | + | chr7.3:40590266-40590285 | MS.gene94266:CDS | 45.0% | |
| GAGGTCACACAGCAAGAAAA+GGG | - | chr7.3:40589739-40589758 | None:intergenic | 45.0% | |
| TCCATGCAGGAATCATACAC+TGG | - | chr7.3:40590008-40590027 | None:intergenic | 45.0% | |
| ! | AGTGAAGCTTTTCGGACTTG+TGG | + | chr7.3:40589416-40589435 | MS.gene94266:CDS | 45.0% |
| ! | CGAGCCAAGGCTCTTTTATA+AGG | - | chr7.3:40589669-40589688 | None:intergenic | 45.0% |
| ! | GTGAAAGCAGTTTTCCATGC+AGG | - | chr7.3:40590021-40590040 | None:intergenic | 45.0% |
| ! | GTGAAGCTTTTCGGACTTGT+GGG | + | chr7.3:40589417-40589436 | MS.gene94266:CDS | 45.0% |
| !!! | TTTTTTCTTTTTTTTTTTAA+TGG | - | chr7.3:40589817-40589836 | None:intergenic | 5.0% |
| CTCAAGGGCAAGTTCTTTGG+TGG | + | chr7.3:40590111-40590130 | MS.gene94266:CDS | 50.0% | |
| GAGCTCAAGGGCAAGTTCTT+TGG | + | chr7.3:40590108-40590127 | MS.gene94266:CDS | 50.0% | |
| ! | AAAGAGCCTTGGCTCGTTTC+TGG | + | chr7.3:40589673-40589692 | MS.gene94266:CDS | 50.0% |
| CTTGGACCAGAAACGAGCCA+AGG | - | chr7.3:40589682-40589701 | None:intergenic | 55.0% | |
| GCCAAAAGCCCTTCTCTACG+AGG | - | chr7.3:40590300-40590319 | None:intergenic | 55.0% | |
| ! | GCCTCGTAGAGAAGGGCTTT+TGG | + | chr7.3:40590296-40590315 | MS.gene94266:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 40589396 | 40590362 | 40589396 | ID=MS.gene94266 |
| chr7.3 | mRNA | 40589396 | 40590362 | 40589396 | ID=MS.gene94266.t1;Parent=MS.gene94266 |
| chr7.3 | exon | 40589396 | 40589716 | 40589396 | ID=MS.gene94266.t1.exon1;Parent=MS.gene94266.t1 |
| chr7.3 | CDS | 40589396 | 40589716 | 40589396 | ID=cds.MS.gene94266.t1;Parent=MS.gene94266.t1 |
| chr7.3 | exon | 40590009 | 40590362 | 40590009 | ID=MS.gene94266.t1.exon2;Parent=MS.gene94266.t1 |
| chr7.3 | CDS | 40590009 | 40590362 | 40590009 | ID=cds.MS.gene94266.t1;Parent=MS.gene94266.t1 |
| Gene Sequence |
| Protein sequence |
