AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05812

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05812.t1 MTR_2g018750 98.522 609 7 1 1 609 1 607 0.0 1237
MS.gene05812.t1 MTR_1g064360 70.628 589 138 7 1 581 1 562 0.0 814
MS.gene05812.t1 MTR_1g094850 53.445 595 265 6 1 589 1 589 0.0 640
MS.gene05812.t1 MTR_2g048390 57.582 455 182 7 1 447 1 452 0.0 525
MS.gene05812.t1 MTR_8g078900 38.623 668 339 18 4 607 1 661 2.27e-148 444
MS.gene05812.t1 MTR_2g084595 38.356 584 320 8 9 564 6 577 6.32e-140 421
MS.gene05812.t1 MTR_2g009340 38.844 623 321 12 1 569 19 635 3.61e-139 421
MS.gene05812.t1 MTR_8g078900 38.110 635 325 17 37 607 1 631 6.15e-136 410
MS.gene05812.t1 MTR_4g093850 37.329 659 347 13 1 609 19 661 3.04e-135 410
MS.gene05812.t1 MTR_8g078900 39.310 580 302 15 74 607 46 621 1.50e-131 399
MS.gene05812.t1 MTR_4g022330 36.581 585 322 12 14 576 23 580 9.82e-118 363
MS.gene05812.t1 MTR_5g016060 35.690 594 332 11 20 604 34 586 9.71e-117 361
MS.gene05812.t1 MTR_1g014070 40.755 503 276 11 1 497 1 487 4.21e-116 359
MS.gene05812.t1 MTR_7g073980 39.083 545 284 13 26 550 33 549 8.66e-116 359
MS.gene05812.t1 MTR_5g032780 40.226 532 279 9 1 502 1 523 3.48e-115 357
MS.gene05812.t1 MTR_8g078900 40.851 470 245 11 4 447 1 463 7.07e-115 351
MS.gene05812.t1 MTR_5g015790 40.358 503 275 12 1 498 1 483 4.06e-110 344
MS.gene05812.t1 MTR_3g062540 35.765 562 277 10 12 561 11 500 6.44e-109 339
MS.gene05812.t1 MTR_1g106430 35.570 596 342 14 1 587 1 563 1.10e-108 340
MS.gene05812.t1 MTR_2g084595 38.053 452 255 5 9 447 6 445 5.84e-108 332
MS.gene05812.t1 MTR_4g093850 39.666 479 250 8 1 447 19 490 1.75e-106 331
MS.gene05812.t1 MTR_8g090220 34.046 608 308 15 26 584 52 615 6.43e-104 328
MS.gene05812.t1 MTR_4g095430 36.382 492 281 9 20 505 7 472 8.65e-104 326
MS.gene05812.t1 MTR_5g075640 35.119 504 279 7 12 505 12 477 7.84e-102 321
MS.gene05812.t1 MTR_8g090220 36.978 503 246 11 26 499 52 512 5.48e-101 317
MS.gene05812.t1 MTR_1g088885 33.649 633 365 14 2 606 13 618 1.64e-99 316
MS.gene05812.t1 MTR_4g134210 35.341 498 295 6 1 496 1 473 1.32e-98 313
MS.gene05812.t1 MTR_8g078900 42.408 382 208 8 74 447 46 423 4.16e-98 306
MS.gene05812.t1 MTR_7g108290 34.992 603 336 16 9 578 20 599 5.92e-98 313
MS.gene05812.t1 MTR_3g062540 34.659 528 262 9 46 561 2 458 4.92e-96 304
MS.gene05812.t1 MTR_8g069330 36.697 545 269 13 1 514 1 500 5.69e-95 303
MS.gene05812.t1 MTR_5g075640 34.468 470 261 6 46 505 2 434 4.26e-90 289
MS.gene05812.t1 MTR_2g027410 34.331 501 286 8 1 496 1 463 2.54e-88 285
MS.gene05812.t1 MTR_5g016060 33.725 510 290 10 104 604 2 472 1.71e-83 271
MS.gene05812.t1 MTR_1g082690 30.556 504 283 8 11 505 43 488 7.35e-83 270
MS.gene05812.t1 MTR_2g027410 34.192 427 240 7 75 496 1 391 4.42e-71 238
MS.gene05812.t1 MTR_4g011530 32.552 384 227 8 206 576 38 402 7.22e-63 214
MS.gene05812.t1 MTR_2g102730 33.894 357 211 4 214 563 24 362 1.52e-59 205
MS.gene05812.t1 MTR_4g045767 27.400 573 334 17 35 573 14 538 3.51e-55 196
MS.gene05812.t1 MTR_4g074190 27.126 494 311 12 29 502 6 470 7.74e-49 179
MS.gene05812.t1 MTR_1g035720 25.303 577 330 17 29 562 6 524 4.31e-48 176
MS.gene05812.t1 MTR_4g074190 27.716 451 279 10 70 502 3 424 5.99e-48 175
MS.gene05812.t1 MTR_4g074190 25.624 441 279 12 29 449 6 417 1.11e-36 142
MS.gene05812.t1 MTR_2g048375 50.382 131 52 2 464 581 1 131 1.80e-32 123
MS.gene05812.t1 MTR_1g105870 33.663 202 115 4 33 233 38 221 1.27e-30 121
MS.gene05812.t1 MTR_2g010890 26.008 496 300 16 94 575 1 443 2.39e-27 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05812.t1 AT5G03250 64.381 598 191 8 1 588 1 586 0.0 785
MS.gene05812.t1 AT1G30440 49.377 642 283 10 1 601 1 641 0.0 620
MS.gene05812.t1 AT5G13600 55.482 602 222 13 1 584 1 574 0.0 613
MS.gene05812.t1 AT5G03250 61.934 486 163 8 113 588 1 474 0.0 607
MS.gene05812.t1 AT5G66560 39.193 694 301 18 1 599 1 668 4.78e-151 451
MS.gene05812.t1 AT3G44820 38.820 644 322 13 8 599 5 628 2.45e-148 443
MS.gene05812.t1 AT5G66560 38.298 658 292 17 37 599 1 639 2.17e-138 417
MS.gene05812.t1 AT5G66560 40.480 583 275 16 70 599 66 629 2.28e-135 409
MS.gene05812.t1 AT3G50840 38.833 600 304 16 18 596 7 564 1.59e-127 387
MS.gene05812.t1 AT5G48800 38.087 596 288 12 14 576 30 577 4.13e-120 369
MS.gene05812.t1 AT5G67385 38.306 496 276 9 12 504 12 480 1.20e-113 352
MS.gene05812.t1 AT3G50840 37.368 570 295 15 47 596 1 528 7.29e-113 348
MS.gene05812.t1 AT3G50840 37.368 570 295 15 47 596 1 528 7.29e-113 348
MS.gene05812.t1 AT5G66560 40.200 500 225 12 1 447 1 479 1.25e-110 340
MS.gene05812.t1 AT3G08570 37.868 544 294 11 26 550 34 552 9.48e-108 337
MS.gene05812.t1 AT1G67900 39.006 523 280 8 1 498 1 509 7.21e-107 335
MS.gene05812.t1 AT1G67900 39.006 523 280 8 1 498 1 509 7.21e-107 335
MS.gene05812.t1 AT1G67900 39.006 523 280 8 1 498 1 509 7.21e-107 335
MS.gene05812.t1 AT1G67900 39.006 523 280 8 1 498 1 509 7.21e-107 335
MS.gene05812.t1 AT1G67900 39.006 523 280 8 1 498 1 509 7.21e-107 335
MS.gene05812.t1 AT1G67900 39.006 523 280 8 1 498 1 509 7.21e-107 335
MS.gene05812.t1 AT4G37590 38.012 513 255 12 1 498 1 465 1.76e-106 333
MS.gene05812.t1 AT1G03010 36.441 590 311 14 24 575 7 570 7.14e-105 330
MS.gene05812.t1 AT1G03010 35.961 609 325 15 5 575 16 597 8.54e-105 330
MS.gene05812.t1 AT1G03010 36.181 597 313 15 20 574 25 595 1.14e-104 330
MS.gene05812.t1 AT4G31820 37.822 505 270 11 1 498 1 468 1.11e-103 325
MS.gene05812.t1 AT2G30520 36.166 506 279 10 4 504 9 475 2.74e-103 325
MS.gene05812.t1 AT5G10250 33.277 589 311 12 12 567 36 575 6.18e-102 322
MS.gene05812.t1 AT3G08660 35.273 550 296 10 24 560 32 534 7.05e-102 321
MS.gene05812.t1 AT2G14820 37.647 510 292 12 1 502 1 492 5.78e-101 320
MS.gene05812.t1 AT2G14820 37.647 510 292 12 1 502 1 492 5.78e-101 320
MS.gene05812.t1 AT2G47860 34.219 602 330 14 5 573 17 585 9.67e-101 320
MS.gene05812.t1 AT5G67385 37.310 461 260 8 47 504 1 435 4.83e-100 315
MS.gene05812.t1 AT5G67385 37.310 461 260 8 47 504 1 435 4.83e-100 315
MS.gene05812.t1 AT5G67385 37.310 461 260 8 47 504 1 435 4.83e-100 315
MS.gene05812.t1 AT5G67385 37.310 461 260 8 47 504 1 435 4.83e-100 315
MS.gene05812.t1 AT5G67440 37.573 511 283 12 1 504 1 482 7.99e-100 315
MS.gene05812.t1 AT5G67440 37.573 511 283 12 1 504 1 482 7.99e-100 315
MS.gene05812.t1 AT5G10250 33.161 579 310 12 12 567 36 560 3.06e-97 310
MS.gene05812.t1 AT2G47860 32.960 625 330 15 5 573 17 608 1.81e-95 306
MS.gene05812.t1 AT2G23050 35.673 513 268 11 1 506 1 458 1.94e-95 301
MS.gene05812.t1 AT3G26490 37.072 526 284 14 1 498 1 507 2.56e-92 296
MS.gene05812.t1 AT3G26490 37.072 526 284 14 1 498 1 507 2.56e-92 296
MS.gene05812.t1 AT3G26490 37.072 526 284 14 1 498 1 507 2.56e-92 296
MS.gene05812.t1 AT3G08660 35.375 506 270 9 66 558 11 472 3.46e-92 294
MS.gene05812.t1 AT2G30520 35.560 464 256 9 46 504 2 427 2.97e-90 290
MS.gene05812.t1 AT2G30520 35.560 464 256 9 46 504 2 427 2.97e-90 290
MS.gene05812.t1 AT5G67440 36.809 470 262 11 41 504 5 445 2.42e-88 285
MS.gene05812.t1 AT5G47800 32.673 505 300 9 1 496 1 474 4.77e-87 281
MS.gene05812.t1 AT5G47800 32.673 505 300 9 1 496 1 474 4.77e-87 281
MS.gene05812.t1 AT5G47800 32.673 505 300 9 1 496 1 474 4.77e-87 281
MS.gene05812.t1 AT5G47800 32.673 505 300 9 1 496 114 587 8.64e-86 281
MS.gene05812.t1 AT4G31820 35.841 452 247 10 53 498 1 415 9.90e-84 271
MS.gene05812.t1 AT3G49970 33.778 450 237 9 36 483 4 394 1.04e-80 264
MS.gene05812.t1 AT3G49970 33.778 450 237 9 36 483 4 394 1.04e-80 264
MS.gene05812.t1 AT3G08660 34.873 433 228 9 24 449 32 417 2.42e-74 244
MS.gene05812.t1 AT2G47860 34.348 460 252 12 5 447 17 443 1.52e-72 240
MS.gene05812.t1 AT3G26490 34.244 476 266 13 1 448 1 457 3.68e-69 233
MS.gene05812.t1 AT2G47860 32.712 483 252 13 5 447 17 466 1.62e-67 227
MS.gene05812.t1 AT2G47860 31.984 494 276 12 113 573 1 467 2.80e-67 228
MS.gene05812.t1 AT2G47860 31.984 494 276 12 113 573 1 467 2.80e-67 228
MS.gene05812.t1 AT5G64330 40.909 286 148 4 7 278 33 311 2.86e-64 223
MS.gene05812.t1 AT5G64330 46.091 243 117 1 276 504 369 611 1.41e-58 208
MS.gene05812.t1 AT5G64330 40.909 286 148 4 7 278 33 311 1.08e-63 223
MS.gene05812.t1 AT5G64330 46.091 243 117 1 276 504 369 611 2.30e-58 209
MS.gene05812.t1 AT2G23050 33.501 397 206 9 113 506 1 342 1.67e-59 203
MS.gene05812.t1 AT5G64330 46.091 243 117 1 276 504 310 552 5.80e-59 208
MS.gene05812.t1 AT5G64330 40.945 254 129 4 39 278 6 252 3.00e-52 189
MS.gene05812.t1 AT1G52770 33.842 393 210 7 214 580 28 396 1.60e-58 203
MS.gene05812.t1 AT5G17580 29.918 488 285 10 28 504 22 463 1.06e-54 195
MS.gene05812.t1 AT3G15570 33.506 385 212 8 209 567 21 387 1.16e-54 192
MS.gene05812.t1 AT3G19850 28.631 482 293 10 36 497 14 464 1.26e-54 195
MS.gene05812.t1 AT5G17580 29.918 488 285 10 28 504 7 448 1.30e-54 195
MS.gene05812.t1 AT3G03510 29.412 510 259 14 82 576 8 431 2.09e-53 189
MS.gene05812.t1 AT3G22104 29.299 471 270 11 22 483 4 420 6.89e-52 186
MS.gene05812.t1 AT5G48130 26.240 625 352 19 19 601 32 589 1.18e-48 180
MS.gene05812.t1 AT1G50280 28.571 476 294 10 35 492 13 460 1.31e-47 175
MS.gene05812.t1 AT1G50280 28.146 437 273 8 70 492 18 427 1.18e-45 169
MS.gene05812.t1 AT3G22104 28.316 392 223 8 100 483 4 345 1.69e-36 142
MS.gene05812.t1 AT3G22104 28.316 392 223 8 100 483 4 345 1.69e-36 142
MS.gene05812.t1 AT3G22104 28.316 392 223 8 100 483 4 345 1.69e-36 142
MS.gene05812.t1 AT3G49900 35.220 159 80 7 20 167 59 205 2.05e-11 67.0
MS.gene05812.t1 AT3G49900 35.220 159 80 7 20 167 59 205 2.32e-11 67.0

Find 125 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATCTTCTCATTCCGAATA+TGG 0.185813 2.3:-67836249 MS.gene05812:CDS
TCTAAACCTGTCTAAATTTC+AGG 0.215961 2.3:-67836018 MS.gene05812:CDS
TCTCCATGTATAACTGAAAT+TGG 0.237203 2.3:-67836134 MS.gene05812:CDS
AGATTGTATTCAGAGGATTT+TGG 0.237388 2.3:-67836198 MS.gene05812:CDS
GCACGTTTCAAGCGCTTTGA+TGG 0.261050 2.3:+67836841 None:intergenic
TGTTATGAGCTCAAATGTTT+TGG 0.294973 2.3:+67837024 None:intergenic
AACTCCCTCAACTACTTTCA+TGG 0.296766 2.3:+67834889 None:intergenic
TATGGTGCAGATAATCTTAT+TGG 0.306224 2.3:-67836911 MS.gene05812:CDS
TTTGGATTTAGAAGAAAATC+AGG 0.306365 2.3:-67834981 MS.gene05812:CDS
TTCTAGATGCATTGATTCCT+TGG 0.328608 2.3:-67836780 MS.gene05812:CDS
TTCCTTCCAATGATAGCTTT+TGG 0.329711 2.3:+67835306 None:intergenic
CACAGCCTGTTGGTGATAAT+TGG 0.336858 2.3:-67836643 MS.gene05812:CDS
CCTCAACTACTTTCATGGTT+TGG 0.344002 2.3:+67834894 None:intergenic
CCCTCACGACGAAATGCTTC+AGG 0.350085 2.3:+67838052 None:intergenic
CGGATCCAGATTTGTTCAAT+TGG 0.351033 2.3:-67836742 MS.gene05812:CDS
ACTTGAACAATTACTCTTAG+AGG 0.351406 2.3:+67835254 None:intergenic
TTTACATTATTTGATTCATT+AGG 0.353297 2.3:+67834948 None:intergenic
CAATGCATCTAGAAACAATA+TGG 0.362633 2.3:+67836789 None:intergenic
AGGCTTTGATCCCAGCTTCA+TGG 0.366193 2.3:+67838072 None:intergenic
GAAATTGGAACATTAATTGC+TGG 0.374348 2.3:-67836119 MS.gene05812:CDS
GATGTGACACCTCTCTTATT+AGG 0.375205 2.3:+67836386 None:intergenic
TTGCAACTTCATGATGTTCC+TGG 0.376919 2.3:-67837052 MS.gene05812:CDS
CTTGGCTATGAAGGCTTGTT+CGG 0.381268 2.3:-67836762 MS.gene05812:CDS
TCAAGCGCTTTGATGGAATC+TGG 0.381679 2.3:+67836848 None:intergenic
TGATCAGCTTCTGAAGTTGT+AGG 0.382260 2.3:+67836443 None:intergenic
ACTTGCCAATTGAACAAATC+TGG 0.382711 2.3:+67836737 None:intergenic
CATTGATTCCTTGGCTATGA+AGG 0.384124 2.3:-67836771 MS.gene05812:CDS
AATTCAAGGGTTTGTACAAC+AGG 0.391467 2.3:-67837832 MS.gene05812:intron
TCTAAATTCTCTTTGCATGA+TGG 0.393396 2.3:+67836311 None:intergenic
GCTGGTAGATAGACATAAAA+TGG 0.403847 2.3:+67836174 None:intergenic
CAAAGAGAATTTAGAGAAAA+GGG 0.406093 2.3:-67836303 MS.gene05812:CDS
GCAAAGAGAATTTAGAGAAA+AGG 0.413793 2.3:-67836304 MS.gene05812:CDS
CGCAATAGATGTATACCTTA+AGG 0.419410 2.3:-67835940 MS.gene05812:intron
TCAAGGGGCCTGGCATAATC+AGG 0.421029 2.3:+67835978 None:intergenic
AGACTTCGCACGTCGATATC+CGG 0.422476 2.3:-67835212 MS.gene05812:CDS
CATCTCTTATATATTACCTT+AGG 0.423118 2.3:-67836526 MS.gene05812:CDS
ATTATTTGATTCATTAGGAT+TGG 0.433862 2.3:+67834953 None:intergenic
CTCCAAATGACTGAAAACTA+TGG 0.440150 2.3:-67836929 MS.gene05812:CDS
TTGCAATTCCTGATTATGCC+AGG 0.447101 2.3:-67835986 MS.gene05812:CDS
TTGTTCAATTGGCAAGTGAC+TGG 0.447348 2.3:-67836731 MS.gene05812:CDS
TGAATCGAAAGGAATGAAAT+CGG 0.448048 2.3:-67836561 MS.gene05812:CDS
AAATGAGTGCTGACCATGTT+TGG 0.451217 2.3:+67838029 None:intergenic
CAACCGAAGGAGATGCTTAG+AGG 0.452313 2.3:+67836364 None:intergenic
AACTTCAGAAGCTGATCAAA+GGG 0.458152 2.3:-67836438 MS.gene05812:CDS
AACCACCAATTATCACCAAC+AGG 0.459595 2.3:+67836638 None:intergenic
GATGACAATGGTCGCTAACT+TGG 0.461208 2.3:-67836078 MS.gene05812:CDS
GATCAGCTTCTGAAGTTGTA+GGG 0.461255 2.3:+67836444 None:intergenic
CACGTAATATCATCGCGGTT+CGG 0.462601 2.3:+67836339 None:intergenic
AAATTGGAACATTAATTGCT+GGG 0.462617 2.3:-67836118 MS.gene05812:CDS
ACTTGATTGTCTATTCATCA+AGG 0.463780 2.3:+67836493 None:intergenic
CTCTTATATATTACCTTAGG+AGG 0.464964 2.3:-67836523 MS.gene05812:CDS
TCGACCGAGTATCCCATATT+CGG 0.470589 2.3:+67836236 None:intergenic
AGGGCAAATATCGGTCCCTC+AGG 0.476825 2.3:-67835146 MS.gene05812:CDS
TTTCCATTACTTTCTAGAAG+TGG 0.477836 2.3:-67837130 MS.gene05812:CDS
ATTAGCATCTGAAGCGACTT+CGG 0.477899 2.3:+67836040 None:intergenic
TGTAATGGCATCCATGAAGC+TGG 0.480977 2.3:-67838083 None:intergenic
ACATACATGGCTGACAGATT+CGG 0.484321 2.3:-67835360 MS.gene05812:CDS
TTCAGATACAAAGAACCAAC+CGG 0.490239 2.3:+67835193 None:intergenic
AGTGATGTTACTATTGAAGT+TGG 0.491007 2.3:-67837802 MS.gene05812:CDS
GAATTGCCAAAAGCTATCAT+TGG 0.494936 2.3:-67835312 MS.gene05812:CDS
GATCTTCTCATTCCGAATAT+GGG 0.495062 2.3:-67836248 MS.gene05812:CDS
ATGGTCGCTAACTTGGTAGA+TGG 0.495307 2.3:-67836071 MS.gene05812:CDS
CAAGTGCCTGAAATTTAGAC+AGG 0.499556 2.3:+67836012 None:intergenic
GAGGAACATGAGTTGTTGAA+TGG 0.502381 2.3:-67836686 MS.gene05812:CDS
GTTCCAATTTCAGTTATACA+TGG 0.505634 2.3:+67836131 None:intergenic
CAAAATCCAAGGGCAAATAT+CGG 0.509120 2.3:-67835155 MS.gene05812:CDS
AGCCTGTTGGTGATAATTGG+TGG 0.510548 2.3:-67836640 MS.gene05812:CDS
TATACCGTCGTCAAGGGGCC+TGG 0.511288 2.3:+67835968 None:intergenic
ATGGCTGACAGATTCGGAAC+GGG 0.514241 2.3:-67835354 MS.gene05812:CDS
GAGGGACCGATATTTGCCCT+TGG 0.518333 2.3:+67835149 None:intergenic
TTGAAAACTCACAAAATCCA+AGG 0.525581 2.3:-67835166 MS.gene05812:CDS
GGGTTTACTAGAAGAAATTG+TGG 0.530266 2.3:-67836417 MS.gene05812:CDS
CAACTTCAGAAGCTGATCAA+AGG 0.539281 2.3:-67836439 MS.gene05812:CDS
CAATTATCACCAACAGGCTG+TGG 0.540795 2.3:+67836644 None:intergenic
CATCCTCTAAGCATCTCCTT+CGG 0.541272 2.3:-67836367 MS.gene05812:CDS
CCAAACCATGAAAGTAGTTG+AGG 0.543035 2.3:-67834894 None:intergenic
GCAGAGTCCACAAGTGAGGA+TGG 0.543325 2.3:-67837088 MS.gene05812:CDS
TATACATGGAGAAGCAGATA+AGG 0.544758 2.3:+67836145 None:intergenic
CAAACCATGAAAGTAGTTGA+GGG 0.548852 2.3:-67834893 None:intergenic
CATTCCGAATATGGGATACT+CGG 0.549415 2.3:-67836240 MS.gene05812:CDS
AAGAGAACGTCTATTAGAGT+TGG 0.549510 2.3:-67835084 MS.gene05812:CDS
GGAGAAGCAGATAAGGATGC+AGG 0.551751 2.3:+67836152 None:intergenic
ACTAGACCAGGCTTCACTTG+TGG 0.556499 2.3:-67836270 MS.gene05812:CDS
AGTCCACTTCTAGAAAGTAA+TGG 0.557324 2.3:+67837127 None:intergenic
GTGGTATATACCGTCGTCAA+GGG 0.566981 2.3:+67835962 None:intergenic
CAACTTCATGATGTTCCTGG+TGG 0.568004 2.3:-67837049 MS.gene05812:CDS
GAAGATCCACAAGTGAAGCC+TGG 0.572388 2.3:+67836264 None:intergenic
CTTGATTGTCTATTCATCAA+GGG 0.575589 2.3:+67836494 None:intergenic
GTCATGCAGCACAAAATGAA+AGG 0.578796 2.3:-67835280 MS.gene05812:CDS
TCCCTCAGGGAGTTGTCAAG+TGG 0.579187 2.3:-67835132 MS.gene05812:CDS
GAGAATTTAGAGAAAAGGGT+AGG 0.579452 2.3:-67836299 MS.gene05812:CDS
TGCCAAAAGCTATCATTGGA+AGG 0.584984 2.3:-67835308 MS.gene05812:CDS
TTGGATTTAGAAGAAAATCA+GGG 0.588050 2.3:-67834980 MS.gene05812:CDS
GCCTGAAGCATTTCGTCGTG+AGG 0.588579 2.3:-67838053 MS.gene05812:CDS
ACTTCAATAGTAACATCACT+TGG 0.592157 2.3:+67837805 None:intergenic
ACTTGATCCATCCTCACTTG+TGG 0.592828 2.3:+67837081 None:intergenic
TCAAGGGAAGAAACCTCCTA+AGG 0.594510 2.3:+67836510 None:intergenic
GGTAGGAGCTCAACTAGACC+AGG 0.597267 2.3:-67836282 MS.gene05812:CDS
AAGCAGATAAGGATGCAGGC+TGG 0.598744 2.3:+67836156 None:intergenic
ATGATATAGATTGTATTCAG+AGG 0.601793 2.3:-67836205 MS.gene05812:CDS
GTAATGGCATCCATGAAGCT+GGG 0.602173 2.3:-67838082 MS.gene05812:CDS
TCCACTTGACAACTCCCTGA+GGG 0.607481 2.3:+67835131 None:intergenic
TCGCGGTTCGGAGCAACCGA+AGG 0.609645 2.3:+67836351 None:intergenic
CCTGAAGCATTTCGTCGTGA+GGG 0.613504 2.3:-67838052 MS.gene05812:CDS
TACGTTGACACCGATGACAA+TGG 0.615680 2.3:-67836090 MS.gene05812:CDS
CGTGGTATATACCGTCGTCA+AGG 0.618223 2.3:+67835961 None:intergenic
CGAACAAGCCTTCATAGCCA+AGG 0.624649 2.3:+67836763 None:intergenic
TTCGTCGTGAGGGCCAAACA+TGG 0.625414 2.3:-67838042 MS.gene05812:intron
TTCGCACGTCGATATCCGGT+TGG 0.629496 2.3:-67835208 MS.gene05812:CDS
CATGGCTGACAGATTCGGAA+CGG 0.631652 2.3:-67835355 MS.gene05812:CDS
TGAAAACTCACAAAATCCAA+GGG 0.635633 2.3:-67835165 MS.gene05812:CDS
AAGTTAGCGACCATTGTCAT+CGG 0.635927 2.3:+67836080 None:intergenic
TGGTATATACCGTCGTCAAG+GGG 0.643375 2.3:+67835963 None:intergenic
GGGCAAATATCGGTCCCTCA+GGG 0.648762 2.3:-67835145 MS.gene05812:CDS
TATGCCAGGCCCCTTGACGA+CGG 0.651135 2.3:-67835972 MS.gene05812:CDS
AATTGCAGAGTCCACAAGTG+AGG 0.652804 2.3:-67837092 MS.gene05812:CDS
GAGTTGATTCCTAATAAGAG+AGG 0.668136 2.3:-67836395 MS.gene05812:CDS
AATACAAAAGTACCTTGTGG+AGG 0.673725 2.3:+67837764 None:intergenic
AATTGGAACATTAATTGCTG+GGG 0.676903 2.3:-67836117 MS.gene05812:CDS
ATCCACTTGACAACTCCCTG+AGG 0.676982 2.3:+67835130 None:intergenic
TAAGGTATACATCTATTGCG+TGG 0.691492 2.3:+67835943 None:intergenic
CAAAGCGCTTGAAACGTGCG+AGG 0.701306 2.3:-67836837 MS.gene05812:CDS
ACTTCAGAAGCTGATCAAAG+GGG 0.702354 2.3:-67836437 MS.gene05812:CDS
GAAAGTAAACCACAGCCTGT+TGG 0.711849 2.3:-67836653 MS.gene05812:CDS
ACTTGCACGTAATATCATCG+CGG 0.804434 2.3:+67836334 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATCAAATGAAATTTA+AGG + chr2.3:67835071-67835090 None:intergenic 10.0%
!! AAAAATTTAATTATAAATGC+TGG + chr2.3:67835736-67835755 None:intergenic 10.0%
!!! AAAATCAATCTTTTATATAA+TGG + chr2.3:67836388-67836407 None:intergenic 10.0%
!!! TAAATTTCATTTGATTTTTT+TGG - chr2.3:67835071-67835090 MS.gene05812:CDS 10.0%
!! AAAACTTAAATCATAAACAA+TGG - chr2.3:67835344-67835363 MS.gene05812:CDS 15.0%
!! AGTTCATATAAAAAAATTAC+TGG + chr2.3:67837088-67837107 None:intergenic 15.0%
!! CACATATTATATAAGATATA+AGG + chr2.3:67834990-67835009 None:intergenic 15.0%
!! TTTACATTATTTGATTCATT+AGG + chr2.3:67838030-67838049 None:intergenic 15.0%
!!! AATTAAATTTTTGTCTTTGT+TGG - chr2.3:67835744-67835763 MS.gene05812:intron 15.0%
!!! TAATCTGTGATTTTTATTTA+GGG + chr2.3:67837244-67837263 None:intergenic 15.0%
!!! TTAATCTGTGATTTTTATTT+AGG + chr2.3:67837245-67837264 None:intergenic 15.0%
!! AAAATGTAACAAAAAACTTC+AGG - chr2.3:67837591-67837610 MS.gene05812:intron 20.0%
!! ATAAACTAACAAAGTATGTT+TGG - chr2.3:67837477-67837496 MS.gene05812:intron 20.0%
!! ATGGTTATAATAAGAAAAAG+AGG - chr2.3:67837447-67837466 MS.gene05812:intron 20.0%
!! ATTATTTGATTCATTAGGAT+TGG + chr2.3:67838025-67838044 None:intergenic 20.0%
!! GAACTTTAGTACTATATATA+CGG + chr2.3:67835472-67835491 None:intergenic 20.0%
!! TAACAAAAACAAAGAAAAGT+TGG - chr2.3:67837953-67837972 MS.gene05812:intron 20.0%
!!! ATGATCTTGTTTTAATTCAA+GGG - chr2.3:67835130-67835149 MS.gene05812:CDS 20.0%
!!! TGTTACATTTTGAAATGATT+AGG + chr2.3:67837582-67837601 None:intergenic 20.0%
!!! TTAAAGTATCTAATTCATGT+TGG - chr2.3:67835785-67835804 MS.gene05812:intron 20.0%
!!! TTGTTCTAATAATTAACTTG+AGG + chr2.3:67835301-67835320 None:intergenic 20.0%
! AAAAAATTACTGGCAAAGAA+AGG + chr2.3:67837078-67837097 None:intergenic 25.0%
! AAATTGGAACATTAATTGCT+GGG - chr2.3:67836857-67836876 MS.gene05812:CDS 25.0%
! AGAAACATCTATACATAGAA+GGG - chr2.3:67837373-67837392 MS.gene05812:intron 25.0%
! ATGATATAGATTGTATTCAG+AGG - chr2.3:67836770-67836789 MS.gene05812:CDS 25.0%
! CAAAGAGAATTTAGAGAAAA+GGG - chr2.3:67836672-67836691 MS.gene05812:CDS 25.0%
! CATCTCTTATATATTACCTT+AGG - chr2.3:67836449-67836468 MS.gene05812:CDS 25.0%
! CTTGAATTAAAACAAGATCA+TGG + chr2.3:67835131-67835150 None:intergenic 25.0%
! TAGAAACATCTATACATAGA+AGG - chr2.3:67837372-67837391 MS.gene05812:intron 25.0%
! TATGTACAAATTGCAAAACA+TGG - chr2.3:67837428-67837447 MS.gene05812:intron 25.0%
! TCATATATAGACACATAGTA+GGG + chr2.3:67835562-67835581 None:intergenic 25.0%
! TTGGATTTAGAAGAAAATCA+GGG - chr2.3:67837995-67838014 MS.gene05812:intron 25.0%
! TTTGGATTTAGAAGAAAATC+AGG - chr2.3:67837994-67838013 MS.gene05812:intron 25.0%
!! AGCATTTTTCCAAAAAGATT+TGG - chr2.3:67837976-67837995 MS.gene05812:intron 25.0%
!! CTTCTAAATCCAAATCTTTT+TGG + chr2.3:67837988-67838007 None:intergenic 25.0%
!! TAAATGAAGTTTTCAGCAAT+TGG - chr2.3:67836104-67836123 MS.gene05812:CDS 25.0%
!! TCAATCTTTTATATAATGGC+AGG + chr2.3:67836384-67836403 None:intergenic 25.0%
!!! ATTTTAGCTATTGAATCGAA+AGG - chr2.3:67836403-67836422 MS.gene05812:CDS 25.0%
!!! ATTTTTTTGGATAGTGAGAA+TGG - chr2.3:67835084-67835103 MS.gene05812:CDS 25.0%
!!! CATGATCTTGTTTTAATTCA+AGG - chr2.3:67835129-67835148 MS.gene05812:CDS 25.0%
!!! TTAAATACAAAAGTACCTTG+TGG + chr2.3:67835217-67835236 None:intergenic 25.0%
!!! TTAAGGTTCTATTTTGACAT+AGG + chr2.3:67835054-67835073 None:intergenic 25.0%
!!! TTTTTTTGGATAGTGAGAAT+GGG - chr2.3:67835085-67835104 MS.gene05812:CDS 25.0%
AATTGGAACATTAATTGCTG+GGG - chr2.3:67836858-67836877 MS.gene05812:CDS 30.0%
ACTTCAATAGTAACATCACT+TGG + chr2.3:67835173-67835192 None:intergenic 30.0%
ACTTGAACAATTACTCTTAG+AGG + chr2.3:67837724-67837743 None:intergenic 30.0%
ACTTGATTGTCTATTCATCA+AGG + chr2.3:67836485-67836504 None:intergenic 30.0%
AGTACATGATTGTGATTTCA+TGG + chr2.3:67837529-67837548 None:intergenic 30.0%
AGTGATGTTACTATTGAAGT+TGG - chr2.3:67835173-67835192 MS.gene05812:CDS 30.0%
CAATGCATCTAGAAACAATA+TGG + chr2.3:67836189-67836208 None:intergenic 30.0%
CATATATAGACACATAGTAG+GGG + chr2.3:67835561-67835580 None:intergenic 30.0%
CATGGTACTAAATTTGTGAA+AGG + chr2.3:67837511-67837530 None:intergenic 30.0%
CTCATATATAGACACATAGT+AGG + chr2.3:67835563-67835582 None:intergenic 30.0%
CTCTTATATATTACCTTAGG+AGG - chr2.3:67836452-67836471 MS.gene05812:CDS 30.0%
CTTGATTGTCTATTCATCAA+GGG + chr2.3:67836484-67836503 None:intergenic 30.0%
GAAATTGGAACATTAATTGC+TGG - chr2.3:67836856-67836875 MS.gene05812:CDS 30.0%
GATGTACATAATGTTGAACA+TGG + chr2.3:67835586-67835605 None:intergenic 30.0%
GCAAAGAGAATTTAGAGAAA+AGG - chr2.3:67836671-67836690 MS.gene05812:CDS 30.0%
GTTCCAATTTCAGTTATACA+TGG + chr2.3:67836847-67836866 None:intergenic 30.0%
TACAAGATGATCTAACCTTA+AGG + chr2.3:67837053-67837072 None:intergenic 30.0%
TATGGTGCAGATAATCTTAT+TGG - chr2.3:67836064-67836083 MS.gene05812:CDS 30.0%
TCTAAACCTGTCTAAATTTC+AGG - chr2.3:67836957-67836976 MS.gene05812:CDS 30.0%
TCTAAATTCTCTTTGCATGA+TGG + chr2.3:67836667-67836686 None:intergenic 30.0%
TCTCCATGTATAACTGAAAT+TGG - chr2.3:67836841-67836860 MS.gene05812:CDS 30.0%
TGAAAACTCACAAAATCCAA+GGG - chr2.3:67837810-67837829 MS.gene05812:CDS 30.0%
TGAATCGAAAGGAATGAAAT+CGG - chr2.3:67836414-67836433 MS.gene05812:CDS 30.0%
TTGAAAACTCACAAAATCCA+AGG - chr2.3:67837809-67837828 MS.gene05812:CDS 30.0%
! AGATTGTATTCAGAGGATTT+TGG - chr2.3:67836777-67836796 MS.gene05812:CDS 30.0%
! CTCATAACAAAGTTTTGCTA+TGG - chr2.3:67835965-67835984 MS.gene05812:CDS 30.0%
! TGTTATGAGCTCAAATGTTT+TGG + chr2.3:67835954-67835973 None:intergenic 30.0%
! TTCAAGGTTGTAAGATGTTT+TGG - chr2.3:67837120-67837139 MS.gene05812:CDS 30.0%
! TTTCCATTACTTTCTAGAAG+TGG - chr2.3:67835845-67835864 MS.gene05812:intron 30.0%
AAAAACTTCAGGCACATACA+TGG - chr2.3:67837602-67837621 MS.gene05812:intron 35.0%
AACTCCTTTCTTATGCTTCA+AGG - chr2.3:67837104-67837123 MS.gene05812:CDS 35.0%
AACTTCAGAAGCTGATCAAA+GGG - chr2.3:67836537-67836556 MS.gene05812:CDS 35.0%
AAGAGAACGTCTATTAGAGT+TGG - chr2.3:67837891-67837910 MS.gene05812:intron 35.0%
AATTCAAGGGTTTGTACAAC+AGG - chr2.3:67835143-67835162 MS.gene05812:CDS 35.0%
AATTGCACCCACTAATTCAT+TGG + chr2.3:67835628-67835647 None:intergenic 35.0%
ACAACCTTGAAGCATAAGAA+AGG + chr2.3:67837111-67837130 None:intergenic 35.0%
ACAATACCAATGAACCGTAT+CGG - chr2.3:67835500-67835519 MS.gene05812:intron 35.0%
ACGAAATTATAGTCACAGAG+AGG + chr2.3:67835411-67835430 None:intergenic 35.0%
ACTTGCCAATTGAACAAATC+TGG + chr2.3:67836241-67836260 None:intergenic 35.0%
ATTGCACCCACTAATTCATT+GGG + chr2.3:67835627-67835646 None:intergenic 35.0%
ATTTGTGATGATTTCGATGC+TGG - chr2.3:67837194-67837213 MS.gene05812:intron 35.0%
CAAAATCCAAGGGCAAATAT+CGG - chr2.3:67837820-67837839 MS.gene05812:CDS 35.0%
CGCAATAGATGTATACCTTA+AGG - chr2.3:67837035-67837054 MS.gene05812:CDS 35.0%
CTCCAAATGACTGAAAACTA+TGG - chr2.3:67836046-67836065 MS.gene05812:CDS 35.0%
GAATTGCCAAAAGCTATCAT+TGG - chr2.3:67837663-67837682 MS.gene05812:intron 35.0%
GAGAATTTAGAGAAAAGGGT+AGG - chr2.3:67836676-67836695 MS.gene05812:CDS 35.0%
GATCGTTTGTTTACGTAGTT+CGG - chr2.3:67835372-67835391 MS.gene05812:intron 35.0%
GATCTTCTCATTCCGAATAT+GGG - chr2.3:67836727-67836746 MS.gene05812:CDS 35.0%
GCTGGTAGATAGACATAAAA+TGG + chr2.3:67836804-67836823 None:intergenic 35.0%
GGGTTTACTAGAAGAAATTG+TGG - chr2.3:67836558-67836577 MS.gene05812:CDS 35.0%
TAAGGTATACATCTATTGCG+TGG + chr2.3:67837035-67837054 None:intergenic 35.0%
TAATGACGCGAAAAATCAAG+AGG - chr2.3:67836270-67836289 MS.gene05812:CDS 35.0%
TATACATGGAGAAGCAGATA+AGG + chr2.3:67836833-67836852 None:intergenic 35.0%
TTCAGATACAAAGAACCAAC+CGG + chr2.3:67837785-67837804 None:intergenic 35.0%
TTTACGAAGAACTAAGATCG+TGG - chr2.3:67837158-67837177 MS.gene05812:intron 35.0%
! AATTTCTTTTCTCCTCCACA+AGG - chr2.3:67835199-67835218 MS.gene05812:CDS 35.0%
! AGTCCACTTCTAGAAAGTAA+TGG + chr2.3:67835851-67835870 None:intergenic 35.0%
! CACCATAGTTTTCAGTCATT+TGG + chr2.3:67836051-67836070 None:intergenic 35.0%
! CTTGATTTTTCGCGTCATTA+CGG + chr2.3:67836270-67836289 None:intergenic 35.0%
! GAGTTGATTCCTAATAAGAG+AGG - chr2.3:67836580-67836599 MS.gene05812:CDS 35.0%
! TTCCTTCCAATGATAGCTTT+TGG + chr2.3:67837672-67837691 None:intergenic 35.0%
!! AATACAAAAGTACCTTGTGG+AGG + chr2.3:67835214-67835233 None:intergenic 35.0%
!! TTCTAGATGCATTGATTCCT+TGG - chr2.3:67836195-67836214 MS.gene05812:CDS 35.0%
AACCACCAATTATCACCAAC+AGG + chr2.3:67836340-67836359 None:intergenic 40.0%
AAGTTAGCGACCATTGTCAT+CGG + chr2.3:67836898-67836917 None:intergenic 40.0%
ACATACATGGCTGACAGATT+CGG - chr2.3:67837615-67837634 MS.gene05812:intron 40.0%
ACTTCAGAAGCTGATCAAAG+GGG - chr2.3:67836538-67836557 MS.gene05812:CDS 40.0%
ACTTGCACGTAATATCATCG+CGG + chr2.3:67836644-67836663 None:intergenic 40.0%
CAACTTCAGAAGCTGATCAA+AGG - chr2.3:67836536-67836555 MS.gene05812:CDS 40.0%
CAAGTGCCTGAAATTTAGAC+AGG + chr2.3:67836966-67836985 None:intergenic 40.0%
CATTCCGAATATGGGATACT+CGG - chr2.3:67836735-67836754 MS.gene05812:CDS 40.0%
GAGGAACATGAGTTGTTGAA+TGG - chr2.3:67836289-67836308 MS.gene05812:CDS 40.0%
GATGTGACACCTCTCTTATT+AGG + chr2.3:67836592-67836611 None:intergenic 40.0%
GCGATACCCAATGAATTAGT+GGG - chr2.3:67835618-67835637 MS.gene05812:intron 40.0%
GGATCTTCTCATTCCGAATA+TGG - chr2.3:67836726-67836745 MS.gene05812:CDS 40.0%
GTCATGCAGCACAAAATGAA+AGG - chr2.3:67837695-67837714 MS.gene05812:intron 40.0%
GTCTAAGCTGCTCGAAAAAT+AGG + chr2.3:67837747-67837766 None:intergenic 40.0%
TGCCAAAAGCTATCATTGGA+AGG - chr2.3:67837667-67837686 MS.gene05812:intron 40.0%
TGCGATACCCAATGAATTAG+TGG - chr2.3:67835617-67835636 MS.gene05812:intron 40.0%
TTGCAACTTCATGATGTTCC+TGG - chr2.3:67835923-67835942 MS.gene05812:intron 40.0%
TTGCAATTCCTGATTATGCC+AGG - chr2.3:67836989-67837008 MS.gene05812:CDS 40.0%
TTGTTCAATTGGCAAGTGAC+TGG - chr2.3:67836244-67836263 MS.gene05812:CDS 40.0%
! ATTAGCATCTGAAGCGACTT+CGG + chr2.3:67836938-67836957 None:intergenic 40.0%
! CGGATCCAGATTTGTTCAAT+TGG - chr2.3:67836233-67836252 MS.gene05812:CDS 40.0%
!! AAATGAGTGCTGACCATGTT+TGG + chr2.3:67834949-67834968 None:intergenic 40.0%
!! CATTGATTCCTTGGCTATGA+AGG - chr2.3:67836204-67836223 MS.gene05812:CDS 40.0%
!! GATCAGCTTCTGAAGTTGTA+GGG + chr2.3:67836534-67836553 None:intergenic 40.0%
!! TGATCAGCTTCTGAAGTTGT+AGG + chr2.3:67836535-67836554 None:intergenic 40.0%
AATTGCAGAGTCCACAAGTG+AGG - chr2.3:67835883-67835902 MS.gene05812:intron 45.0%
ACGAAGAACTAAGATCGTGG+AGG - chr2.3:67837161-67837180 MS.gene05812:intron 45.0%
ACTTGATCCATCCTCACTTG+TGG + chr2.3:67835897-67835916 None:intergenic 45.0%
ATACCAATGAACCGTATCGG+AGG - chr2.3:67835503-67835522 MS.gene05812:intron 45.0%
ATAGACACATAGTAGGGGCA+AGG + chr2.3:67835556-67835575 None:intergenic 45.0%
ATGGTCGCTAACTTGGTAGA+TGG - chr2.3:67836904-67836923 MS.gene05812:CDS 45.0%
CAACTTCATGATGTTCCTGG+TGG - chr2.3:67835926-67835945 MS.gene05812:intron 45.0%
CAATTATCACCAACAGGCTG+TGG + chr2.3:67836334-67836353 None:intergenic 45.0%
CACGTAATATCATCGCGGTT+CGG + chr2.3:67836639-67836658 None:intergenic 45.0%
CATCCTCTAAGCATCTCCTT+CGG - chr2.3:67836608-67836627 MS.gene05812:CDS 45.0%
GAAAGTAAACCACAGCCTGT+TGG - chr2.3:67836322-67836341 MS.gene05812:CDS 45.0%
GATGACAATGGTCGCTAACT+TGG - chr2.3:67836897-67836916 MS.gene05812:CDS 45.0%
GTGGTATATACCGTCGTCAA+GGG + chr2.3:67837016-67837035 None:intergenic 45.0%
TACCAATGAACCGTATCGGA+GGG - chr2.3:67835504-67835523 MS.gene05812:intron 45.0%
TACGTTGACACCGATGACAA+TGG - chr2.3:67836885-67836904 MS.gene05812:CDS 45.0%
TAGACACATAGTAGGGGCAA+GGG + chr2.3:67835555-67835574 None:intergenic 45.0%
TCAAGCGCTTTGATGGAATC+TGG + chr2.3:67836130-67836149 None:intergenic 45.0%
TCAAGGGAAGAAACCTCCTA+AGG + chr2.3:67836468-67836487 None:intergenic 45.0%
TCGACCGAGTATCCCATATT+CGG + chr2.3:67836742-67836761 None:intergenic 45.0%
TGGTATATACCGTCGTCAAG+GGG + chr2.3:67837015-67837034 None:intergenic 45.0%
! CTTGGCTATGAAGGCTTGTT+CGG - chr2.3:67836213-67836232 MS.gene05812:CDS 45.0%
!! AGCCTGTTGGTGATAATTGG+TGG - chr2.3:67836335-67836354 MS.gene05812:CDS 45.0%
!! CACAGCCTGTTGGTGATAAT+TGG - chr2.3:67836332-67836351 MS.gene05812:CDS 45.0%
!!! TCAAATGTTTTGGCTCCACC+AGG + chr2.3:67835944-67835963 None:intergenic 45.0%
AAGCAGATAAGGATGCAGGC+TGG + chr2.3:67836822-67836841 None:intergenic 50.0%
ACTAGACCAGGCTTCACTTG+TGG - chr2.3:67836705-67836724 MS.gene05812:CDS 50.0%
AGACACATAGTAGGGGCAAG+GGG + chr2.3:67835554-67835573 None:intergenic 50.0%
AGACTTCGCACGTCGATATC+CGG - chr2.3:67837763-67837782 MS.gene05812:intron 50.0%
ATCCACTTGACAACTCCCTG+AGG + chr2.3:67837848-67837867 None:intergenic 50.0%
ATGGCTGACAGATTCGGAAC+GGG - chr2.3:67837621-67837640 MS.gene05812:intron 50.0%
CAACCGAAGGAGATGCTTAG+AGG + chr2.3:67836614-67836633 None:intergenic 50.0%
CATGGCTGACAGATTCGGAA+CGG - chr2.3:67837620-67837639 MS.gene05812:intron 50.0%
CGAACAAGCCTTCATAGCCA+AGG + chr2.3:67836215-67836234 None:intergenic 50.0%
CGTGGTATATACCGTCGTCA+AGG + chr2.3:67837017-67837036 None:intergenic 50.0%
GAAGATCCACAAGTGAAGCC+TGG + chr2.3:67836714-67836733 None:intergenic 50.0%
GCACGTTTCAAGCGCTTTGA+TGG + chr2.3:67836137-67836156 None:intergenic 50.0%
GGAGAAGCAGATAAGGATGC+AGG + chr2.3:67836826-67836845 None:intergenic 50.0%
TAACGTAGCTCCCTCCGATA+CGG + chr2.3:67835517-67835536 None:intergenic 50.0%
TCCACTTGACAACTCCCTGA+GGG + chr2.3:67837847-67837866 None:intergenic 50.0%
! AGGCTTTGATCCCAGCTTCA+TGG + chr2.3:67834906-67834925 None:intergenic 50.0%
! CCTGAAGCATTTCGTCGTGA+GGG - chr2.3:67834923-67834942 MS.gene05812:CDS 50.0%
AGGGCAAATATCGGTCCCTC+AGG - chr2.3:67837829-67837848 MS.gene05812:intron 55.0%
CCCTCACGACGAAATGCTTC+AGG + chr2.3:67834926-67834945 None:intergenic 55.0%
CTCCCTCCGATACGGTTCAT+TGG + chr2.3:67835509-67835528 None:intergenic 55.0%
GAGGGACCGATATTTGCCCT+TGG + chr2.3:67837829-67837848 None:intergenic 55.0%
GCAGAGTCCACAAGTGAGGA+TGG - chr2.3:67835887-67835906 MS.gene05812:intron 55.0%
GGGCAAATATCGGTCCCTCA+GGG - chr2.3:67837830-67837849 MS.gene05812:intron 55.0%
GGTAGGAGCTCAACTAGACC+AGG - chr2.3:67836693-67836712 MS.gene05812:CDS 55.0%
TCAAGGGGCCTGGCATAATC+AGG + chr2.3:67837000-67837019 None:intergenic 55.0%
TCCCTCAGGGAGTTGTCAAG+TGG - chr2.3:67837843-67837862 MS.gene05812:intron 55.0%
TTCGCACGTCGATATCCGGT+TGG - chr2.3:67837767-67837786 MS.gene05812:intron 55.0%
TTCGTCGTGAGGGCCAAACA+TGG - chr2.3:67834933-67834952 MS.gene05812:CDS 55.0%
! CAAAGCGCTTGAAACGTGCG+AGG - chr2.3:67836138-67836157 MS.gene05812:CDS 55.0%
! GCCTGAAGCATTTCGTCGTG+AGG - chr2.3:67834922-67834941 MS.gene05812:CDS 55.0%
ACATAGTAGGGGCAAGGGGC+GGG + chr2.3:67835550-67835569 None:intergenic 60.0%
CACATAGTAGGGGCAAGGGG+CGG + chr2.3:67835551-67835570 None:intergenic 60.0%
TATACCGTCGTCAAGGGGCC+TGG + chr2.3:67837010-67837029 None:intergenic 60.0%
TATGCCAGGCCCCTTGACGA+CGG - chr2.3:67837003-67837022 MS.gene05812:CDS 60.0%
ATAGTAGGGGCAAGGGGCGG+GGG + chr2.3:67835548-67835567 None:intergenic 65.0%
CATAGTAGGGGCAAGGGGCG+GGG + chr2.3:67835549-67835568 None:intergenic 65.0%
! TCGCGGTTCGGAGCAACCGA+AGG + chr2.3:67836627-67836646 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 67834896 67838101 67834896 ID=MS.gene05812
chr2.3 mRNA 67834896 67838101 67834896 ID=MS.gene05812.t1;Parent=MS.gene05812
chr2.3 exon 67838043 67838101 67838043 ID=MS.gene05812.t1.exon1;Parent=MS.gene05812.t1
chr2.3 CDS 67838043 67838101 67838043 ID=cds.MS.gene05812.t1;Parent=MS.gene05812.t1
chr2.3 exon 67837777 67837846 67837777 ID=MS.gene05812.t1.exon2;Parent=MS.gene05812.t1
chr2.3 CDS 67837777 67837846 67837777 ID=cds.MS.gene05812.t1;Parent=MS.gene05812.t1
chr2.3 exon 67835941 67837152 67835941 ID=MS.gene05812.t1.exon3;Parent=MS.gene05812.t1
chr2.3 CDS 67835941 67837152 67835941 ID=cds.MS.gene05812.t1;Parent=MS.gene05812.t1
chr2.3 exon 67834896 67835384 67834896 ID=MS.gene05812.t1.exon4;Parent=MS.gene05812.t1
chr2.3 CDS 67834896 67835384 67834896 ID=cds.MS.gene05812.t1;Parent=MS.gene05812.t1
Gene Sequence

>MS.gene05812

ATGGCATCCATGAAGCTGGGATCAAAGCCTGAAGCATTTCGTCGTGAGGGCCAAACATGGTCAGCACTCATTTCTCTATATCAATATTCATCCTTATATCTTATATAATATGTGACATTAACAATGTGTGTAATTTTTTTATTTCTGTCAATGTTCCTATGTCAAAATAGAACCTTAAATTTCATTTGATTTTTTTGGATAGTGAGAATGGGTTTCTCAATTGTATAATTGACCATGATCTTGTTTTAATTCAAGGGTTTGTACAACAGGACTTCCAAGTGATGTTACTATTGAAGTTGGCGAAATTTCTTTTCTCCTCCACAAGGTACTTTTGTATTTAACTTTAAAACTTATTGCACACTTTTTCTTAATGTAACATTAGTTAATTTATTTCAATTTTATCCTCAAGTTAATTATTAGAACAACTACTAGTAACAGATTATTAAAGAAAACTTAAATCATAAACAATGGAATGTGATCGTTTGTTTACGTAGTTCGGTCAATTGTGTATGCCTCTCTGTGACTATAATTTCGTATATTACTTGAGTGAATTAGTGTTCAAAGTGTTACAAGCCGTATATATAGTACTAAAGTTCAGATTACAACAATACCAATGAACCGTATCGGAGGGAGCTACGTTAGCTCAGCTCCCCCGCCCCTTGCCCCTACTATGTGTCTATATATGAGCCATGTTCAACATTATGTACATCGTCAAATTTTGTGCGATACCCAATGAATTAGTGGGTGCAATTTTTAAAATATCGAGACTCGTTCAAATTGCAACAAAAGTACAAAAATACAGTGAATTGTGTGTTAAAAGAGTGTTTTTCTAAATTTCCAGCATTTATAATTAAATTTTTGTCTTTGTTGGTCTATGCAACATGTTAAATTAAAGTATCTAATTCATGTTGGTCATTAGTTTTTTTCAACATTTGTGATTTTCATGCAGTTTCCATTACTTTCTAGAAGTGGACTACTAAAGAAACTAATTGCAGAGTCCACAAGTGAGGATGGATCAAGTAGTGTTTTGCAACTTCATGATGTTCCTGGTGGAGCCAAAACATTTGAGCTCATAACAAAGTTTTGCTATGGTGTGAAATTAGAAATCACAGCATTGAATGTAGTTAGTCTAAGATGTGCAGCAGAGTATCTCCAAATGACTGAAAACTATGGTGCAGATAATCTTATTGGTCACACAGAATCTTTTCTAAATGAAGTTTTCAGCAATTGGCCAGATTCCATCAAAGCGCTTGAAACGTGCGAGGAAGTGCAAAACTTTGCAGAAGATCTCCATATTGTTTCTAGATGCATTGATTCCTTGGCTATGAAGGCTTGTTCGGATCCAGATTTGTTCAATTGGCAAGTGACTGGCCGTAATGACGCGAAAAATCAAGAGGAACATGAGTTGTTGAATGGAATGTCTTGTGAAAGTAAACCACAGCCTGTTGGTGATAATTGGTGGTTTTATGATGTGTCTTTGTTGAGCCTGCCATTATATAAAAGATTGATTTTAGCTATTGAATCGAAAGGAATGAAATCGGAGACTATTGCTGCATCTCTTATATATTACCTTAGGAGGTTTCTTCCCTTGATGAATAGACAATCAAGTTTCAAAGATACAAATCATGCAACAATCCCTACAACTTCAGAAGCTGATCAAAGGGGTTTACTAGAAGAAATTGTGGAGTTGATTCCTAATAAGAGAGGTGTCACATCCTCTAAGCATCTCCTTCGGTTGCTCCGAACCGCGATGATATTACGTGCAAGTCCATCATGCAAAGAGAATTTAGAGAAAAGGGTAGGAGCTCAACTAGACCAGGCTTCACTTGTGGATCTTCTCATTCCGAATATGGGATACTCGGTCGAGACGCTCTATGATATAGATTGTATTCAGAGGATTTTGGACCATTTTATGTCTATCTACCAGCCTGCATCCTTATCTGCTTCTCCATGTATAACTGAAATTGGAACATTAATTGCTGGGGTTGATACGTTGACACCGATGACAATGGTCGCTAACTTGGTAGATGGATATCTCGCCGAAGTCGCTTCAGATGCTAATCTAAACCTGTCTAAATTTCAGGCACTTGCTGTTGCAATTCCTGATTATGCCAGGCCCCTTGACGACGGTATATACCACGCAATAGATGTATACCTTAAGGTTAGATCATCTTGTATACCTTTCTTTGCCAGTAATTTTTTTATATGAACTCCTTTCTTATGCTTCAAGGTTGTAAGATGTTTTGGCATAATAATAAAAAGTTTACGAAGAACTAAGATCGTGGAGGAAATAATGAGATTTGTGATGATTTCGATGCTGGTGAATTAATGAATCAAAAAATTGTCCCTAAATAAAAATCACAGATTAAATTTCTCAGTACATTTTTTTCTTCTCAAAATATACTTCTAATTACTGCTACTAAATGTTGAGAGATGAGAAGTAAGAGTTGAAGATATCATATACAAGATGATATTTTAGAAACATCTATACATAGAAGGGACATGTTAAATATACTACTACTTTGTTTAATATATGTACAAATTGCAAAACATGGTTATAATAAGAAAAAGAGGTAGCAAAATAAACTAACAAAGTATGTTTGGTGAATGAACCTTTCACAAATTTAGTACCATGAAATCACAATCATGTACTAATTAAAATTTACAATTGTTTTCTTTTGTTCCTAATCATTTCAAAATGTAACAAAAAACTTCAGGCACATACATGGCTGACAGATTCGGAACGGGAACAATTATGCAGACTAATGAATTGCCAAAAGCTATCATTGGAAGGAAGCAGTCATGCAGCACAAAATGAAAGGTTGCCTCTAAGAGTAATTGTTCAAGTCCTATTTTTCGAGCAGCTTAGACTTCGCACGTCGATATCCGGTTGGTTCTTTGTATCTGAAAATCTTGAAAACTCACAAAATCCAAGGGCAAATATCGGTCCCTCAGGGAGTTGTCAAGTGGATTCTGCAGAGACAACTGAGAATGTAAGAGAACGTCTATTAGAGTTGGAGAAAGAATGTTCAAGCATTCGAAAAGAGTTGCAGAAGCTAACAAAAACAAAGAAAAGTTGGAGCATTTTTCCAAAAAGATTTGGATTTAGAAGAAAATCAGGGTCTTCCAATCCTAATGAATCAAATAATGTAAATACATCATCATCATCATCTGTGAATAAAAATCCAAACCATGAAAGTAGTTGA
Protein sequence

>MS.gene05812.t1

MASMKLGSKPEAFRREGQTWVCTTGLPSDVTIEVGEISFLLHKFPLLSRSGLLKKLIAESTSEDGSSSVLQLHDVPGGAKTFELITKFCYGVKLEITALNVVSLRCAAEYLQMTENYGADNLIGHTESFLNEVFSNWPDSIKALETCEEVQNFAEDLHIVSRCIDSLAMKACSDPDLFNWQVTGRNDAKNQEEHELLNGMSCESKPQPVGDNWWFYDVSLLSLPLYKRLILAIESKGMKSETIAASLIYYLRRFLPLMNRQSSFKDTNHATIPTTSEADQRGLLEEIVELIPNKRGVTSSKHLLRLLRTAMILRASPSCKENLEKRVGAQLDQASLVDLLIPNMGYSVETLYDIDCIQRILDHFMSIYQPASLSASPCITEIGTLIAGVDTLTPMTMVANLVDGYLAEVASDANLNLSKFQALAVAIPDYARPLDDGIYHAIDVYLKAHTWLTDSEREQLCRLMNCQKLSLEGSSHAAQNERLPLRVIVQVLFFEQLRLRTSISGWFFVSENLENSQNPRANIGPSGSCQVDSAETTENVRERLLELEKECSSIRKELQKLTKTKKSWSIFPKRFGFRRKSGSSNPNESNNVNTSSSSSVNKNPNHESS