Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058768.t1 | XP_003598033.2 | 96.8 | 493 | 15 | 1 | 3 | 495 | 49 | 540 | 3.30E-258 | 900.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058768.t1 | Q9FVW4 | 48.2 | 498 | 191 | 12 | 3 | 495 | 47 | 482 | 1.1e-97 | 358.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058768.t1 | G7IYF2 | 96.8 | 493 | 15 | 1 | 3 | 495 | 49 | 540 | 2.4e-258 | 900.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene30557 | MS.gene058768 | PPI |
| MS.gene033864 | MS.gene058768 | PPI |
| MS.gene021158 | MS.gene058768 | PPI |
| MS.gene41129 | MS.gene058768 | PPI |
| MS.gene069519 | MS.gene058768 | PPI |
| MS.gene003999 | MS.gene058768 | PPI |
| MS.gene75866 | MS.gene058768 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058768.t1 | MTR_3g005510 | 96.559 | 494 | 16 | 1 | 2 | 495 | 48 | 540 | 0.0 | 965 |
| MS.gene058768.t1 | MTR_2g005600 | 41.551 | 503 | 235 | 18 | 10 | 495 | 50 | 510 | 3.47e-84 | 269 |
| MS.gene058768.t1 | MTR_2g005600 | 39.587 | 533 | 233 | 18 | 10 | 495 | 50 | 540 | 1.23e-81 | 263 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058768.t1 | AT1G33980 | 48.503 | 501 | 187 | 14 | 2 | 495 | 46 | 482 | 7.47e-122 | 365 |
| MS.gene058768.t1 | AT1G33980 | 48.303 | 501 | 188 | 14 | 2 | 495 | 48 | 484 | 3.69e-121 | 363 |
Find 115 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATCCCCGTCTGGTATTAC+TGG | 0.217254 | 3.1:+745769 | None:intergenic |
| GAGTTTGCGGAGTTCTTTAA+TGG | 0.236801 | 3.1:-748231 | MS.gene058768:CDS |
| TTCCACCGGCTTCACGCTTT+TGG | 0.251325 | 3.1:+741474 | None:intergenic |
| AAATTTATATGTGCAGTTAT+AGG | 0.264693 | 3.1:-748308 | None:intergenic |
| GGTAAAAGAAAATGCTTATT+CGG | 0.273466 | 3.1:-740916 | MS.gene058768:CDS |
| ATTCTGAAGGGGAATCTAAA+AGG | 0.281820 | 3.1:-741277 | MS.gene058768:CDS |
| TCTTCTGAATCAAGTATAAA+AGG | 0.294483 | 3.1:-743983 | MS.gene058768:CDS |
| CTTGCCTTGCATAGCAGCTC+TGG | 0.315043 | 3.1:+745799 | None:intergenic |
| AGGCGTCCACCTCGTCCTTT+TGG | 0.319628 | 3.1:-741353 | MS.gene058768:CDS |
| GTCATACCTTCAACTGATTC+AGG | 0.322351 | 3.1:+741068 | None:intergenic |
| TCTGTATGCAGAAACAAGTT+TGG | 0.325968 | 3.1:-740526 | MS.gene058768:intron |
| GCTGCTATGCAAGGCAAGCC+TGG | 0.337012 | 3.1:-745794 | MS.gene058768:CDS |
| CAGGCCTGATCGTGGTGTTT+GGG | 0.338312 | 3.1:-741171 | MS.gene058768:CDS |
| CCATCATCCTTCACTATATA+GGG | 0.349986 | 3.1:+740676 | None:intergenic |
| ACAGGCCTGATCGTGGTGTT+TGG | 0.351241 | 3.1:-741172 | MS.gene058768:CDS |
| GGCATTGAAGGTTCCGTCTC+TGG | 0.374822 | 3.1:-741977 | MS.gene058768:CDS |
| ACCATCATCCTTCACTATAT+AGG | 0.376532 | 3.1:+740675 | None:intergenic |
| AGGGGAATCTAAAAGGGATT+TGG | 0.379049 | 3.1:-741270 | MS.gene058768:CDS |
| AAAATGCTTATTCGGGAAAC+AGG | 0.394937 | 3.1:-740908 | MS.gene058768:CDS |
| ACGGGGATCTGAAAAGAAAA+AGG | 0.396521 | 3.1:-745756 | MS.gene058768:intron |
| AGAAGAATGGTCCTCACGCC+TGG | 0.398858 | 3.1:+744000 | None:intergenic |
| TGGGTTCAGAAATCATCTTC+AGG | 0.399182 | 3.1:-740506 | MS.gene058768:CDS |
| CTGTATGCAGAAACAAGTTT+GGG | 0.400640 | 3.1:-740525 | MS.gene058768:intron |
| CGTGAAGGGACTATATATAA+AGG | 0.410366 | 3.1:-747501 | MS.gene058768:intron |
| TTGAGGAACTCGAGATAGTC+TGG | 0.416889 | 3.1:+747359 | None:intergenic |
| GTAAAAGAAAATGCTTATTC+GGG | 0.425492 | 3.1:-740915 | MS.gene058768:CDS |
| TCCAAAGCCGAGTACCAAAA+AGG | 0.427328 | 3.1:-747529 | MS.gene058768:CDS |
| TCCGAGTGCAGAAATACAAT+TGG | 0.428949 | 3.1:-747309 | MS.gene058768:CDS |
| CAAATCTTAACTTCTGAGAA+TGG | 0.431636 | 3.1:-741377 | MS.gene058768:CDS |
| AAACTTTCAAAGAAAGGTGT+TGG | 0.453145 | 3.1:-740631 | MS.gene058768:CDS |
| CTTTCAGGCCACTGAAGGTA+TGG | 0.458031 | 3.1:-741555 | MS.gene058768:intron |
| GATCCCCGTCTGGTATTACT+GGG | 0.459889 | 3.1:+745770 | None:intergenic |
| CATCTTGTGTCAGGTTTCAT+CGG | 0.460552 | 3.1:-745846 | MS.gene058768:intron |
| TATACAGTGCATGGCATTGA+AGG | 0.462061 | 3.1:-741989 | MS.gene058768:CDS |
| GAGGCTTTGAAATGAGTTTG+AGG | 0.464705 | 3.1:+747342 | None:intergenic |
| AACTTTCAAAGAAAGGTGTT+GGG | 0.465614 | 3.1:-740630 | MS.gene058768:CDS |
| TTATACTTGATTCAGAAGAA+TGG | 0.467658 | 3.1:+743987 | None:intergenic |
| ATTACTAGAATGTGAAGAAT+CGG | 0.475433 | 3.1:+741133 | None:intergenic |
| CTGTTTAACCATACCTTCAG+TGG | 0.475898 | 3.1:+741547 | None:intergenic |
| CGTCTGGTATTACTGGGGCC+AGG | 0.479425 | 3.1:+745776 | None:intergenic |
| TCTGACGTATATATGCCATC+AGG | 0.484252 | 3.1:+747083 | None:intergenic |
| AGAAATACAATTGGAAAGAA+AGG | 0.484703 | 3.1:-747300 | MS.gene058768:CDS |
| CTTTATATATAGTCCCTTCA+CGG | 0.485953 | 3.1:+747502 | None:intergenic |
| GGTGAATTCTCAACTTCTGC+AGG | 0.489502 | 3.1:-740882 | MS.gene058768:CDS |
| GCCATCAGGGGTGTGACAAT+AGG | 0.490885 | 3.1:+747097 | None:intergenic |
| TTCTGAAGGGGAATCTAAAA+GGG | 0.490928 | 3.1:-741276 | MS.gene058768:CDS |
| GCAAACTCGAAAACATCATC+AGG | 0.492867 | 3.1:+748246 | None:intergenic |
| ACAATGTCAAGAATGCTAAT+CGG | 0.510501 | 3.1:-744060 | MS.gene058768:CDS |
| TCATATCCTGAATCAGTTGA+AGG | 0.510630 | 3.1:-741074 | MS.gene058768:intron |
| CAGAACCAAAAGCGTGAAGC+CGG | 0.512531 | 3.1:-741479 | MS.gene058768:CDS |
| AAGCCGGTGGAAGATTGATC+AGG | 0.514442 | 3.1:-741463 | MS.gene058768:CDS |
| GGTGCCCAAACACCACGATC+AGG | 0.517553 | 3.1:+741167 | None:intergenic |
| GAAGAATGGTCCTCACGCCT+GGG | 0.520144 | 3.1:+744001 | None:intergenic |
| TCCAATTGTATTTCTGCACT+CGG | 0.526926 | 3.1:+747308 | None:intergenic |
| TCATCGGCCGCAACAAGAGT+TGG | 0.528330 | 3.1:-745830 | MS.gene058768:CDS |
| GAAGGGAAACTTTCAAAGAA+AGG | 0.528593 | 3.1:-740637 | MS.gene058768:CDS |
| TGCTGTTGACAGTGGTCCAC+TGG | 0.532809 | 3.1:-747056 | MS.gene058768:intron |
| CCCTATATAGTGAAGGATGA+TGG | 0.537091 | 3.1:-740676 | MS.gene058768:CDS |
| AATCCAGACCTTGAACCAAA+AGG | 0.539385 | 3.1:+741338 | None:intergenic |
| CTGCCTTTACTGTGGTCCCC+AGG | 0.539742 | 3.1:-744018 | MS.gene058768:CDS |
| ACGCCTGGGGACCACAGTAA+AGG | 0.540090 | 3.1:+744015 | None:intergenic |
| TGGTTGTCAGTATGTTCAGA+AGG | 0.540774 | 3.1:-744083 | MS.gene058768:intron |
| CTCCTTTCTAGGGGCTAGCA+AGG | 0.541253 | 3.1:-747125 | MS.gene058768:intron |
| TCAACTGATTCAGGATATGA+TGG | 0.541254 | 3.1:+741077 | None:intergenic |
| ACCTATTGTCACACCCCTGA+TGG | 0.547863 | 3.1:-747098 | MS.gene058768:CDS |
| ACTCAACTACTGCTTTATGC+TGG | 0.548532 | 3.1:+747571 | None:intergenic |
| AGTAGTCCCTCTGTTGAGAA+TGG | 0.556313 | 3.1:-740852 | MS.gene058768:intron |
| CTGACGTATATATGCCATCA+GGG | 0.556567 | 3.1:+747084 | None:intergenic |
| CATGTCTCTAGTCATGATGC+TGG | 0.556919 | 3.1:-741308 | MS.gene058768:CDS |
| CCTTCTGAAAGGAAAACAGC+GGG | 0.562206 | 3.1:-741915 | MS.gene058768:CDS |
| AGAGCCAGAGCTGCTATGCA+AGG | 0.562280 | 3.1:-745803 | MS.gene058768:CDS |
| TCTTAACTTCTGAGAATGGA+AGG | 0.562840 | 3.1:-741373 | MS.gene058768:CDS |
| GTGCAGTCAGTTCGAGTGAA+GGG | 0.563790 | 3.1:-740654 | MS.gene058768:CDS |
| GGCTTTGGAACACGCTGAGA+AGG | 0.570985 | 3.1:+747543 | None:intergenic |
| TACTGTGGTCCCCAGGCGTG+AGG | 0.571346 | 3.1:-744011 | MS.gene058768:CDS |
| TGGAACTTTCAGGCCACTGA+AGG | 0.573276 | 3.1:-741560 | MS.gene058768:intron |
| TGGCCCCAGTAATACCAGAC+GGG | 0.576005 | 3.1:-745774 | MS.gene058768:CDS |
| GCTGGGCATGCTAATTCTGA+AGG | 0.580615 | 3.1:-741290 | MS.gene058768:CDS |
| GCTCCTGATCAATCTTCCAC+CGG | 0.582381 | 3.1:+741460 | None:intergenic |
| TGAGCTCTCATCATCCTCAC+TGG | 0.585263 | 3.1:-741111 | MS.gene058768:CDS |
| AGAAAGGAAGCTGAGCAAGC+TGG | 0.587029 | 3.1:-747284 | MS.gene058768:intron |
| CACCAACCATTCTCAACAGA+GGG | 0.588071 | 3.1:+740846 | None:intergenic |
| GCACTCGGAAGATGTTCTTG+AGG | 0.590407 | 3.1:+747323 | None:intergenic |
| GGTGCAGTCAGTTCGAGTGA+AGG | 0.593819 | 3.1:-740655 | MS.gene058768:CDS |
| CTGGGCATGCTAATTCTGAA+GGG | 0.594470 | 3.1:-741289 | MS.gene058768:CDS |
| TCTTCTGCCAACTCTTGTTG+CGG | 0.594967 | 3.1:+745823 | None:intergenic |
| ATGTCTCTAGTCATGATGCT+GGG | 0.599416 | 3.1:-741307 | MS.gene058768:CDS |
| AACCAAAAGCGTGAAGCCGG+TGG | 0.606148 | 3.1:-741476 | MS.gene058768:CDS |
| GAATTCTCAACTTCTGCAGG+TGG | 0.610336 | 3.1:-740879 | MS.gene058768:CDS |
| TTTCCTGAGTCAGAAGTCAG+TGG | 0.613047 | 3.1:+741950 | None:intergenic |
| ATACCACTGACTTCTGACTC+AGG | 0.622853 | 3.1:-741953 | MS.gene058768:CDS |
| CTGGCCCCAGTAATACCAGA+CGG | 0.624233 | 3.1:-745775 | MS.gene058768:CDS |
| GACGTACTAGAAACAAGGAC+AGG | 0.626561 | 3.1:-741190 | MS.gene058768:CDS |
| TGGGGCTCCCTATATAGTGA+AGG | 0.637270 | 3.1:-740683 | MS.gene058768:CDS |
| ATTGATGAATATACAGTGCA+TGG | 0.638195 | 3.1:-741998 | MS.gene058768:CDS |
| AACAAGGACAGGCCTGATCG+TGG | 0.639778 | 3.1:-741179 | MS.gene058768:CDS |
| AGTCAGTGGTATTCCAGAGA+CGG | 0.644574 | 3.1:+741964 | None:intergenic |
| CAGAAGCGTGCTGTTGACAG+TGG | 0.645146 | 3.1:-747064 | MS.gene058768:CDS |
| TGGGCATGCTAATTCTGAAG+GGG | 0.646561 | 3.1:-741288 | MS.gene058768:CDS |
| GACCTTGAACCAAAAGGACG+AGG | 0.650876 | 3.1:+741344 | None:intergenic |
| TGTTGGGAATAGTACTCATG+AGG | 0.651580 | 3.1:-740614 | MS.gene058768:intron |
| GCTTATTCGGGAAACAGGAG+TGG | 0.655321 | 3.1:-740903 | MS.gene058768:CDS |
| AGAAAGACGTACTAGAAACA+AGG | 0.658009 | 3.1:-741195 | MS.gene058768:CDS |
| AAGAATGGTCCTCACGCCTG+GGG | 0.661060 | 3.1:+744002 | None:intergenic |
| TCCTTCTGAAAGGAAAACAG+CGG | 0.668874 | 3.1:-741916 | MS.gene058768:CDS |
| CTTGAACCAAAAGGACGAGG+TGG | 0.668946 | 3.1:+741347 | None:intergenic |
| GAAGAATCGGAACGGCGAAG+AGG | 0.673314 | 3.1:+741146 | None:intergenic |
| ACACCAACCATTCTCAACAG+AGG | 0.678931 | 3.1:+740845 | None:intergenic |
| AGACAAATCTGCCTTTACTG+TGG | 0.679939 | 3.1:-744026 | MS.gene058768:CDS |
| GGCCCCAGTAATACCAGACG+GGG | 0.682231 | 3.1:-745773 | MS.gene058768:CDS |
| TAGAATGTGAAGAATCGGAA+CGG | 0.692170 | 3.1:+741138 | None:intergenic |
| GCAGTAGATGTAGACTGACT+CGG | 0.696602 | 3.1:+741413 | None:intergenic |
| TGACGTATATATGCCATCAG+GGG | 0.713880 | 3.1:+747085 | None:intergenic |
| ATCCCCGTCTGGTATTACTG+GGG | 0.722921 | 3.1:+745771 | None:intergenic |
| TGGTGCAGACTGTGCCAGTG+AGG | 0.729152 | 3.1:+741097 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 740499 | 748322 | 740499 | ID=MS.gene058768 |
| chr3.1 | mRNA | 740499 | 748322 | 740499 | ID=MS.gene058768.t1;Parent=MS.gene058768 |
| chr3.1 | exon | 748208 | 748322 | 748208 | ID=MS.gene058768.t1.exon1;Parent=MS.gene058768.t1 |
| chr3.1 | CDS | 748208 | 748322 | 748208 | ID=cds.MS.gene058768.t1;Parent=MS.gene058768.t1 |
| chr3.1 | exon | 747502 | 747597 | 747502 | ID=MS.gene058768.t1.exon2;Parent=MS.gene058768.t1 |
| chr3.1 | CDS | 747502 | 747597 | 747502 | ID=cds.MS.gene058768.t1;Parent=MS.gene058768.t1 |
| chr3.1 | exon | 747285 | 747383 | 747285 | ID=MS.gene058768.t1.exon3;Parent=MS.gene058768.t1 |
| chr3.1 | CDS | 747285 | 747383 | 747285 | ID=cds.MS.gene058768.t1;Parent=MS.gene058768.t1 |
| chr3.1 | exon | 747057 | 747136 | 747057 | ID=MS.gene058768.t1.exon4;Parent=MS.gene058768.t1 |
| chr3.1 | CDS | 747057 | 747136 | 747057 | ID=cds.MS.gene058768.t1;Parent=MS.gene058768.t1 |
| chr3.1 | exon | 745757 | 745855 | 745757 | ID=MS.gene058768.t1.exon5;Parent=MS.gene058768.t1 |
| chr3.1 | CDS | 745757 | 745855 | 745757 | ID=cds.MS.gene058768.t1;Parent=MS.gene058768.t1 |
| chr3.1 | exon | 743963 | 744095 | 743963 | ID=MS.gene058768.t1.exon6;Parent=MS.gene058768.t1 |
| chr3.1 | CDS | 743963 | 744095 | 743963 | ID=cds.MS.gene058768.t1;Parent=MS.gene058768.t1 |
| chr3.1 | exon | 741904 | 742028 | 741904 | ID=MS.gene058768.t1.exon7;Parent=MS.gene058768.t1 |
| chr3.1 | CDS | 741904 | 742028 | 741904 | ID=cds.MS.gene058768.t1;Parent=MS.gene058768.t1 |
| chr3.1 | exon | 741075 | 741570 | 741075 | ID=MS.gene058768.t1.exon8;Parent=MS.gene058768.t1 |
| chr3.1 | CDS | 741075 | 741570 | 741075 | ID=cds.MS.gene058768.t1;Parent=MS.gene058768.t1 |
| chr3.1 | exon | 740853 | 740942 | 740853 | ID=MS.gene058768.t1.exon9;Parent=MS.gene058768.t1 |
| chr3.1 | CDS | 740853 | 740942 | 740853 | ID=cds.MS.gene058768.t1;Parent=MS.gene058768.t1 |
| chr3.1 | exon | 740615 | 740730 | 740615 | ID=MS.gene058768.t1.exon10;Parent=MS.gene058768.t1 |
| chr3.1 | CDS | 740615 | 740730 | 740615 | ID=cds.MS.gene058768.t1;Parent=MS.gene058768.t1 |
| chr3.1 | exon | 740499 | 740537 | 740499 | ID=MS.gene058768.t1.exon11;Parent=MS.gene058768.t1 |
| chr3.1 | CDS | 740499 | 740537 | 740499 | ID=cds.MS.gene058768.t1;Parent=MS.gene058768.t1 |
| Gene Sequence |
| Protein sequence |
