Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30725.t1 | XP_013452188.1 | 79.3 | 116 | 17 | 1 | 1 | 109 | 22 | 137 | 8.20E-45 | 189.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30725.t1 | P62929 | 40.6 | 64 | 38 | 0 | 41 | 104 | 66 | 129 | 7.3e-10 | 64.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30725.t1 | A0A072U9W9 | 79.3 | 116 | 17 | 1 | 1 | 109 | 22 | 137 | 5.9e-45 | 189.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene30725 | 0.813762 | 2.17E-51 | -1.69E-46 |
| MS.gene05240 | MS.gene30725 | 0.839514 | 1.51E-57 | -1.69E-46 |
| MS.gene052702 | MS.gene30725 | 0.832365 | 9.86E-56 | -1.69E-46 |
| MS.gene053031 | MS.gene30725 | 0.819199 | 1.32E-52 | -1.69E-46 |
| MS.gene054181 | MS.gene30725 | 0.859833 | 3.15E-63 | -1.69E-46 |
| MS.gene054742 | MS.gene30725 | 0.815627 | 8.39E-52 | -1.69E-46 |
| MS.gene056590 | MS.gene30725 | 0.816512 | 5.32E-52 | -1.69E-46 |
| MS.gene056591 | MS.gene30725 | 0.814229 | 1.71E-51 | -1.69E-46 |
| MS.gene057544 | MS.gene30725 | 0.806164 | 9.40E-50 | -1.69E-46 |
| MS.gene059401 | MS.gene30725 | 0.814 | 1.93E-51 | -1.69E-46 |
| MS.gene059402 | MS.gene30725 | 0.867015 | 1.87E-65 | -1.69E-46 |
| MS.gene059955 | MS.gene30725 | 0.814776 | 1.30E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30725.t1 | MTR_6g047880 | 79.310 | 116 | 17 | 1 | 1 | 109 | 22 | 137 | 2.52e-61 | 184 |
| MS.gene30725.t1 | MTR_6g047900 | 75.221 | 113 | 24 | 3 | 1 | 109 | 22 | 134 | 6.27e-55 | 167 |
| MS.gene30725.t1 | MTR_7g029540 | 61.224 | 98 | 33 | 2 | 1 | 93 | 24 | 121 | 1.61e-35 | 118 |
| MS.gene30725.t1 | MTR_3g463570 | 49.541 | 109 | 48 | 3 | 1 | 103 | 23 | 130 | 7.45e-23 | 86.3 |
| MS.gene30725.t1 | MTR_3g067270 | 43.636 | 110 | 56 | 2 | 1 | 105 | 22 | 130 | 1.14e-22 | 85.9 |
| MS.gene30725.t1 | MTR_3g467750 | 43.636 | 110 | 56 | 2 | 1 | 105 | 22 | 130 | 1.14e-22 | 85.9 |
| MS.gene30725.t1 | MTR_3g463570 | 49.541 | 109 | 48 | 3 | 1 | 103 | 43 | 150 | 2.10e-22 | 85.9 |
| MS.gene30725.t1 | MTR_3g067280 | 41.818 | 110 | 58 | 2 | 1 | 105 | 22 | 130 | 3.22e-21 | 82.0 |
| MS.gene30725.t1 | MTR_3g467760 | 47.561 | 82 | 41 | 1 | 24 | 105 | 27 | 106 | 2.43e-20 | 79.3 |
| MS.gene30725.t1 | MTR_7g056817 | 40.870 | 115 | 57 | 4 | 1 | 105 | 22 | 135 | 7.12e-20 | 79.0 |
| MS.gene30725.t1 | MTR_7g044980 | 44.898 | 98 | 44 | 4 | 1 | 92 | 21 | 114 | 2.51e-19 | 77.8 |
| MS.gene30725.t1 | MTR_6g036620 | 40.000 | 110 | 58 | 3 | 2 | 105 | 23 | 130 | 6.32e-19 | 76.3 |
| MS.gene30725.t1 | MTR_7g056803 | 43.860 | 114 | 54 | 4 | 1 | 105 | 22 | 134 | 6.59e-19 | 76.3 |
| MS.gene30725.t1 | MTR_3g067500 | 38.393 | 112 | 62 | 3 | 1 | 106 | 1 | 111 | 2.98e-18 | 73.9 |
| MS.gene30725.t1 | MTR_3g067437 | 40.000 | 110 | 56 | 3 | 1 | 102 | 22 | 129 | 3.09e-18 | 74.3 |
| MS.gene30725.t1 | MTR_3g067550 | 40.517 | 116 | 57 | 3 | 1 | 105 | 1 | 115 | 2.65e-17 | 71.6 |
| MS.gene30725.t1 | MTR_3g067555 | 40.517 | 116 | 57 | 3 | 1 | 105 | 1 | 115 | 2.65e-17 | 71.6 |
| MS.gene30725.t1 | MTR_3g067540 | 40.517 | 116 | 57 | 3 | 1 | 105 | 22 | 136 | 3.34e-17 | 72.0 |
| MS.gene30725.t1 | MTR_3g067555 | 40.517 | 116 | 57 | 3 | 1 | 105 | 22 | 136 | 3.34e-17 | 72.0 |
| MS.gene30725.t1 | MTR_3g067555 | 40.517 | 116 | 57 | 3 | 1 | 105 | 9 | 123 | 3.39e-17 | 71.6 |
| MS.gene30725.t1 | MTR_6g017150 | 50.746 | 67 | 32 | 1 | 40 | 105 | 67 | 133 | 4.05e-17 | 71.6 |
| MS.gene30725.t1 | MTR_3g436100 | 42.105 | 76 | 43 | 1 | 28 | 102 | 59 | 134 | 6.18e-17 | 71.6 |
| MS.gene30725.t1 | MTR_3g067535 | 40.351 | 114 | 56 | 3 | 1 | 105 | 22 | 132 | 1.97e-16 | 70.1 |
| MS.gene30725.t1 | MTR_3g067570 | 43.158 | 95 | 43 | 4 | 1 | 88 | 1 | 91 | 2.18e-16 | 68.6 |
| MS.gene30725.t1 | MTR_8g022400 | 38.889 | 90 | 53 | 2 | 14 | 102 | 42 | 130 | 6.99e-16 | 68.9 |
| MS.gene30725.t1 | MTR_8g022420 | 42.667 | 75 | 42 | 1 | 29 | 102 | 55 | 129 | 4.43e-15 | 66.6 |
| MS.gene30725.t1 | MTR_3g067580 | 38.393 | 112 | 61 | 4 | 1 | 105 | 30 | 140 | 5.26e-15 | 66.6 |
| MS.gene30725.t1 | MTR_8g022430 | 39.744 | 78 | 46 | 1 | 32 | 108 | 59 | 136 | 6.18e-15 | 66.2 |
| MS.gene30725.t1 | MTR_1g019650 | 43.299 | 97 | 47 | 3 | 1 | 96 | 22 | 111 | 6.61e-15 | 65.5 |
| MS.gene30725.t1 | MTR_6g017170 | 38.235 | 102 | 56 | 3 | 1 | 95 | 22 | 123 | 2.09e-14 | 64.7 |
| MS.gene30725.t1 | MTR_3g067430 | 37.288 | 118 | 58 | 4 | 1 | 105 | 22 | 136 | 3.12e-14 | 64.3 |
| MS.gene30725.t1 | MTR_7g044920 | 33.333 | 117 | 65 | 2 | 1 | 106 | 21 | 135 | 8.11e-14 | 63.5 |
| MS.gene30725.t1 | MTR_4g026590 | 35.135 | 111 | 54 | 3 | 1 | 96 | 23 | 130 | 2.21e-13 | 62.4 |
| MS.gene30725.t1 | MTR_3g067510 | 38.053 | 113 | 54 | 5 | 1 | 105 | 22 | 126 | 3.89e-13 | 61.2 |
| MS.gene30725.t1 | MTR_3g067510 | 38.053 | 113 | 54 | 5 | 1 | 105 | 27 | 131 | 4.33e-13 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTAGTCCTAATGTTGATTA+TGG | 0.176691 | 6.2:-41961868 | MS.gene30725:CDS |
| CACAACCTACACCGCATGTT+TGG | 0.290947 | 6.2:+41962022 | None:intergenic |
| ACATGAGATTGACAAAAGTT+AGG | 0.309257 | 6.2:+41961934 | None:intergenic |
| AGAAGCAAACCGTTGTTATC+TGG | 0.377036 | 6.2:-41962064 | MS.gene30725:CDS |
| ACAACCTACACCGCATGTTT+GGG | 0.389533 | 6.2:+41962023 | None:intergenic |
| CATGAGATTGACAAAAGTTA+GGG | 0.391241 | 6.2:+41961935 | None:intergenic |
| ACAAGATCTTTATATGATGC+AGG | 0.450360 | 6.2:+41961976 | None:intergenic |
| CACAAACCATAATCAACATT+AGG | 0.450638 | 6.2:+41961862 | None:intergenic |
| TTTGCTTCTACTTTCTTCAT+CGG | 0.487039 | 6.2:+41962078 | None:intergenic |
| CTTCTTGACCGTGCTTGAGA+TGG | 0.487144 | 6.2:-41961773 | MS.gene30725:CDS |
| ATCTTCTGCCTCAGATAAAG+AGG | 0.487203 | 6.2:+41961834 | None:intergenic |
| AAAGATCTTGTAAAGATACC+TGG | 0.510848 | 6.2:-41961964 | MS.gene30725:CDS |
| GGTGTAGGTTGTGTTTGTTT+CGG | 0.512311 | 6.2:-41962012 | MS.gene30725:CDS |
| GATCTTTATATGATGCAGGT+TGG | 0.554280 | 6.2:+41961980 | None:intergenic |
| TCATGTGGAATGCAAAGAGA+AGG | 0.561795 | 6.2:-41961917 | MS.gene30725:CDS |
| GTGTGTTGCCTCTTTATCTG+AGG | 0.572934 | 6.2:-41961842 | MS.gene30725:CDS |
| ACATCCCAAACATGCGGTGT+AGG | 0.582167 | 6.2:-41962027 | MS.gene30725:CDS |
| CCATAATCAACATTAGGACT+AGG | 0.583229 | 6.2:+41961868 | None:intergenic |
| AAGATCTTGTAAAGATACCT+GGG | 0.599370 | 6.2:-41961963 | MS.gene30725:CDS |
| CATGTGGAATGCAAAGAGAA+GGG | 0.618807 | 6.2:-41961916 | MS.gene30725:CDS |
| ATATCAAGCCATCTCAAGCA+CGG | 0.656087 | 6.2:+41961765 | None:intergenic |
| TCAGAGACATCCCAAACATG+CGG | 0.712944 | 6.2:-41962033 | MS.gene30725:CDS |
| AACAAACAGCCAGATAACAA+CGG | 0.721899 | 6.2:+41962055 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGATCTTGTAAAGATACC+TGG | - | chr6.2:41961879-41961898 | MS.gene30725:CDS | 30.0% | |
| AAGATCTTGTAAAGATACCT+GGG | - | chr6.2:41961880-41961899 | MS.gene30725:CDS | 30.0% | |
| ACAAGATCTTTATATGATGC+AGG | + | chr6.2:41961870-41961889 | None:intergenic | 30.0% | |
| ACATGAGATTGACAAAAGTT+AGG | + | chr6.2:41961912-41961931 | None:intergenic | 30.0% | |
| CACAAACCATAATCAACATT+AGG | + | chr6.2:41961984-41962003 | None:intergenic | 30.0% | |
| CATGAGATTGACAAAAGTTA+GGG | + | chr6.2:41961911-41961930 | None:intergenic | 30.0% | |
| ! | TAACTTTTGTCAATCTCATG+TGG | - | chr6.2:41961911-41961930 | MS.gene30725:CDS | 30.0% |
| AACAAACAGCCAGATAACAA+CGG | + | chr6.2:41961791-41961810 | None:intergenic | 35.0% | |
| CCATAATCAACATTAGGACT+AGG | + | chr6.2:41961978-41961997 | None:intergenic | 35.0% | |
| GATCTTTATATGATGCAGGT+TGG | + | chr6.2:41961866-41961885 | None:intergenic | 35.0% | |
| ! | CCTAGTCCTAATGTTGATTA+TGG | - | chr6.2:41961975-41961994 | MS.gene30725:CDS | 35.0% |
| ! | TTTTGCGACTGTAGATTTAG+AGG | + | chr6.2:41962048-41962067 | None:intergenic | 35.0% |
| AGAAGCAAACCGTTGTTATC+TGG | - | chr6.2:41961779-41961798 | MS.gene30725:CDS | 40.0% | |
| ATCTTCTGCCTCAGATAAAG+AGG | + | chr6.2:41962012-41962031 | None:intergenic | 40.0% | |
| CATGTGGAATGCAAAGAGAA+GGG | - | chr6.2:41961927-41961946 | MS.gene30725:CDS | 40.0% | |
| GGTGTAGGTTGTGTTTGTTT+CGG | - | chr6.2:41961831-41961850 | MS.gene30725:CDS | 40.0% | |
| TCATGTGGAATGCAAAGAGA+AGG | - | chr6.2:41961926-41961945 | MS.gene30725:CDS | 40.0% | |
| ! | CAAAAGTTAGGGTTTTTCCC+AGG | + | chr6.2:41961900-41961919 | None:intergenic | 40.0% |
| ACAACCTACACCGCATGTTT+GGG | + | chr6.2:41961823-41961842 | None:intergenic | 45.0% | |
| GTGTGTTGCCTCTTTATCTG+AGG | - | chr6.2:41962001-41962020 | MS.gene30725:CDS | 45.0% | |
| TCAGAGACATCCCAAACATG+CGG | - | chr6.2:41961810-41961829 | MS.gene30725:CDS | 45.0% | |
| ACATCCCAAACATGCGGTGT+AGG | - | chr6.2:41961816-41961835 | MS.gene30725:CDS | 50.0% | |
| CACAACCTACACCGCATGTT+TGG | + | chr6.2:41961824-41961843 | None:intergenic | 50.0% | |
| CTTCTTGACCGTGCTTGAGA+TGG | - | chr6.2:41962070-41962089 | MS.gene30725:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 41961768 | 41962097 | 41961768 | ID=MS.gene30725 |
| chr6.2 | mRNA | 41961768 | 41962097 | 41961768 | ID=MS.gene30725.t1;Parent=MS.gene30725 |
| chr6.2 | exon | 41961768 | 41962097 | 41961768 | ID=MS.gene30725.t1.exon1;Parent=MS.gene30725.t1 |
| chr6.2 | CDS | 41961768 | 41962097 | 41961768 | ID=cds.MS.gene30725.t1;Parent=MS.gene30725.t1 |
| Gene Sequence |
| Protein sequence |
