Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059540.t1 | XP_003620790.1 | 79.8 | 89 | 18 | 0 | 1 | 89 | 1 | 89 | 3.60E-22 | 114 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059540.t1 | G7KI13 | 79.8 | 89 | 18 | 0 | 1 | 89 | 1 | 89 | 2.6e-22 | 114.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051568 | MS.gene059540 | -0.810953 | 8.92E-51 | -1.69E-46 |
| MS.gene059540 | MS.gene071623 | -0.812206 | 4.76E-51 | -1.69E-46 |
| MS.gene059540 | MS.gene071624 | -0.813511 | 2.47E-51 | -1.69E-46 |
| MS.gene059540 | MS.gene53419 | -0.8167 | 4.83E-52 | -1.69E-46 |
| MS.gene059540 | MS.gene61305 | 0.814313 | 1.64E-51 | -1.69E-46 |
| MS.gene059540 | MS.gene68982 | -0.805777 | 1.13E-49 | -1.69E-46 |
| MS.gene059540 | MS.gene71972 | 0.812788 | 3.55E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059540.t1 | MTR_6g090490 | 91.011 | 89 | 8 | 0 | 1 | 89 | 1 | 89 | 1.47e-52 | 159 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCTTGATCTTCGAAATT+TGG | 0.115608 | 6.4:+62090042 | None:intergenic |
| TGGAGCTGCTTTGGATTCTA+AGG | 0.294697 | 6.4:-62090067 | MS.gene059540:CDS |
| TGCAGAACATGGAGCTGCTT+TGG | 0.354322 | 6.4:-62090076 | MS.gene059540:CDS |
| CCTCCGTGACCTCCTTTCTT+CGG | 0.356322 | 6.4:+62090138 | None:intergenic |
| ACCTCCTTTCTTCGGTGAAC+CGG | 0.365180 | 6.4:+62090146 | None:intergenic |
| ACGAGATTCCGATGCGGAGG+AGG | 0.466592 | 6.4:+62090197 | None:intergenic |
| CTTCAGATCGGTGCAGAACA+TGG | 0.498685 | 6.4:-62090087 | MS.gene059540:CDS |
| AAAGATCGCAAATCATCCTC+TGG | 0.510278 | 6.4:-62090174 | MS.gene059540:CDS |
| ACACAACCACCGCAATCAAA+CGG | 0.536314 | 6.4:+62089978 | None:intergenic |
| AAATCATCCTCTGGAATGAC+CGG | 0.538942 | 6.4:-62090165 | MS.gene059540:CDS |
| CAATCAAACGGTAACGGAAG+CGG | 0.553668 | 6.4:+62089990 | None:intergenic |
| CGGAAAATCCTCCTCCGCAT+CGG | 0.554876 | 6.4:-62090205 | MS.gene059540:CDS |
| GTCACGGAGGGAAGTTCACA+TGG | 0.573877 | 6.4:-62090125 | MS.gene059540:CDS |
| AAAAGCCACAAATCCACCAC+CGG | 0.574764 | 6.4:-62090225 | MS.gene059540:CDS |
| GGAGGGAAGTTCACATGGAT+CGG | 0.576604 | 6.4:-62090120 | MS.gene059540:CDS |
| TGTTCTGCACCGATCTGAAG+AGG | 0.576691 | 6.4:+62090090 | None:intergenic |
| ATGACCGGTTCACCGAAGAA+AGG | 0.587391 | 6.4:-62090150 | MS.gene059540:CDS |
| AGCGGTGACAGTGTCAGTGT+CGG | 0.604133 | 6.4:+62090008 | None:intergenic |
| GGATCTGATCCTCTTCAGAT+CGG | 0.604184 | 6.4:-62090099 | MS.gene059540:CDS |
| CGAAGAAAGGAGGTCACGGA+GGG | 0.619061 | 6.4:-62090137 | MS.gene059540:CDS |
| GGTGACAGTGTCAGTGTCGG+CGG | 0.620679 | 6.4:+62090011 | None:intergenic |
| ACTTCAACAATGAAATCCAC+CGG | 0.621434 | 6.4:-62090249 | None:intergenic |
| CGGTGAACCGGTCATTCCAG+AGG | 0.635019 | 6.4:+62090158 | None:intergenic |
| TCACCGAAGAAAGGAGGTCA+CGG | 0.636627 | 6.4:-62090141 | MS.gene059540:CDS |
| TTTACGAGATTCCGATGCGG+AGG | 0.641309 | 6.4:+62090194 | None:intergenic |
| ACCGGTTCACCGAAGAAAGG+AGG | 0.641392 | 6.4:-62090147 | MS.gene059540:CDS |
| CCACCGCAATCAAACGGTAA+CGG | 0.649561 | 6.4:+62089984 | None:intergenic |
| CCGAAGAAAGGAGGTCACGG+AGG | 0.673021 | 6.4:-62090138 | MS.gene059540:CDS |
| ATCTTTACGAGATTCCGATG+CGG | 0.748893 | 6.4:+62090191 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TCTTCTTGATCTTCGAAATT+TGG | + | chr6.4:62090194-62090213 | None:intergenic | 30.0% | |
| AAAGATCGCAAATCATCCTC+TGG | - | chr6.4:62090059-62090078 | MS.gene059540:CDS | 40.0% | |
| ATCTTTACGAGATTCCGATG+CGG | + | chr6.4:62090045-62090064 | None:intergenic | 40.0% | |
| !! | AAATCATCCTCTGGAATGAC+CGG | - | chr6.4:62090068-62090087 | MS.gene059540:CDS | 40.0% |
| AAAAGCCACAAATCCACCAC+CGG | - | chr6.4:62090008-62090027 | MS.gene059540:CDS | 45.0% | |
| GGATCTGATCCTCTTCAGAT+CGG | - | chr6.4:62090134-62090153 | MS.gene059540:CDS | 45.0% | |
| ! | TGGAGCTGCTTTGGATTCTA+AGG | - | chr6.4:62090166-62090185 | MS.gene059540:CDS | 45.0% |
| !! | GGTGGATTTGTGGCTTTTTC+CGG | + | chr6.4:62090006-62090025 | None:intergenic | 45.0% |
| !!! | ATTTTCCGGTGGTGGATTTG+TGG | + | chr6.4:62090016-62090035 | None:intergenic | 45.0% |
| ACCTCCTTTCTTCGGTGAAC+CGG | + | chr6.4:62090090-62090109 | None:intergenic | 50.0% | |
| CTTCAGATCGGTGCAGAACA+TGG | - | chr6.4:62090146-62090165 | MS.gene059540:CDS | 50.0% | |
| GGAGGGAAGTTCACATGGAT+CGG | - | chr6.4:62090113-62090132 | MS.gene059540:CDS | 50.0% | |
| TCACCGAAGAAAGGAGGTCA+CGG | - | chr6.4:62090092-62090111 | MS.gene059540:CDS | 50.0% | |
| TGCAGAACATGGAGCTGCTT+TGG | - | chr6.4:62090157-62090176 | MS.gene059540:CDS | 50.0% | |
| TTTACGAGATTCCGATGCGG+AGG | + | chr6.4:62090042-62090061 | None:intergenic | 50.0% | |
| ! | ATGACCGGTTCACCGAAGAA+AGG | - | chr6.4:62090083-62090102 | MS.gene059540:CDS | 50.0% |
| ! | TGTTCTGCACCGATCTGAAG+AGG | + | chr6.4:62090146-62090165 | None:intergenic | 50.0% |
| !! | GGATTTGTGGCTTTTTCCGG+TGG | + | chr6.4:62090003-62090022 | None:intergenic | 50.0% |
| AGCGGTGACAGTGTCAGTGT+CGG | + | chr6.4:62090228-62090247 | None:intergenic | 55.0% | |
| CCTCCGTGACCTCCTTTCTT+CGG | + | chr6.4:62090098-62090117 | None:intergenic | 55.0% | |
| CGAAGAAAGGAGGTCACGGA+GGG | - | chr6.4:62090096-62090115 | MS.gene059540:CDS | 55.0% | |
| CGGAAAATCCTCCTCCGCAT+CGG | - | chr6.4:62090028-62090047 | MS.gene059540:CDS | 55.0% | |
| GTCACGGAGGGAAGTTCACA+TGG | - | chr6.4:62090108-62090127 | MS.gene059540:CDS | 55.0% | |
| ! | ACCGGTTCACCGAAGAAAGG+AGG | - | chr6.4:62090086-62090105 | MS.gene059540:CDS | 55.0% |
| ! | CGATGCGGAGGAGGATTTTC+CGG | + | chr6.4:62090030-62090049 | None:intergenic | 55.0% |
| ACGAGATTCCGATGCGGAGG+AGG | + | chr6.4:62090039-62090058 | None:intergenic | 60.0% | |
| CCGAAGAAAGGAGGTCACGG+AGG | - | chr6.4:62090095-62090114 | MS.gene059540:CDS | 60.0% | |
| CGGTGAACCGGTCATTCCAG+AGG | + | chr6.4:62090078-62090097 | None:intergenic | 60.0% | |
| ! | GGAGGAGGATTTTCCGGTGG+TGG | + | chr6.4:62090024-62090043 | None:intergenic | 60.0% |
| ! | GGTGACAGTGTCAGTGTCGG+CGG | + | chr6.4:62090225-62090244 | None:intergenic | 60.0% |
| ! | TGCGGAGGAGGATTTTCCGG+TGG | + | chr6.4:62090027-62090046 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 62089993 | 62090262 | 62089993 | ID=MS.gene059540 |
| chr6.4 | mRNA | 62089993 | 62090262 | 62089993 | ID=MS.gene059540.t1;Parent=MS.gene059540 |
| chr6.4 | exon | 62089993 | 62090262 | 62089993 | ID=MS.gene059540.t1.exon1;Parent=MS.gene059540.t1 |
| chr6.4 | CDS | 62089993 | 62090262 | 62089993 | ID=cds.MS.gene059540.t1;Parent=MS.gene059540.t1 |
| Gene Sequence |
| Protein sequence |
