AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059857

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059857.t1 MTR_2g079700 93.525 417 25 1 1 415 1 417 0.0 788
MS.gene059857.t1 MTR_6g007490 64.748 417 145 2 1 415 25 441 0.0 579
MS.gene059857.t1 MTR_6g007490 61.238 307 117 2 1 305 25 331 2.91e-139 402
MS.gene059857.t1 MTR_3g086910 48.974 390 178 7 41 415 32 415 1.58e-116 346
MS.gene059857.t1 MTR_3g086910 47.547 265 123 5 55 305 42 304 8.85e-73 231
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059857.t1 AT1G52920 67.470 415 130 1 1 415 1 410 0.0 583
MS.gene059857.t1 AT2G20770 63.462 416 140 3 1 415 1 405 0.0 549
MS.gene059857.t1 AT5G65280 45.501 389 189 7 49 415 46 433 3.49e-112 336
MS.gene059857.t1 AT5G65280 47.573 309 147 5 121 415 4 311 1.91e-96 291

Find 113 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTAAGATCATCCACATTCT+TGG 0.288609 2.2:+15556755 None:intergenic
ATACCTCTTTGAATTGTCTA+AGG 0.293167 2.2:+15555844 None:intergenic
AGACCTTCGAATAAGGAATA+AGG 0.296203 2.2:+15554696 None:intergenic
CATCCACATTCTTGGTTACT+TGG 0.297066 2.2:+15556763 None:intergenic
TCCTCCAAGACCTTCGAATA+AGG 0.318685 2.2:+15554689 None:intergenic
GGTGCTGCTCATGGACTTGC+AGG 0.320322 2.2:-15555399 MS.gene059857:CDS
CTCCTCCATGCATCTTTCCT+TGG 0.350097 2.2:+15554724 None:intergenic
CGTGCCGGTGTTTGTGCTCT+TGG 0.351585 2.2:-15555915 MS.gene059857:CDS
TTGACATCTTCCACCTCATC+TGG 0.364253 2.2:+15555342 None:intergenic
TATTCCTTATTCGAAGGTCT+TGG 0.377583 2.2:-15554693 MS.gene059857:CDS
CATTGGTGTCACGGTGCTCC+TGG 0.378975 2.2:-15555234 MS.gene059857:CDS
AATGGCATGGGAAGAAATAC+TGG 0.383288 2.2:-15555422 MS.gene059857:CDS
GAGTTATTGTATGGCAGAAC+AGG 0.390773 2.2:-15555688 MS.gene059857:CDS
AGCACTTCACTTCAAACAAT+TGG 0.392054 2.2:-15557302 MS.gene059857:intron
GGTTCTTGGAACGGCATACT+TGG 0.395964 2.2:-15556799 MS.gene059857:CDS
GCTTCTTCAAGTGTTGTCTC+TGG 0.397728 2.2:+15557424 None:intergenic
CAGGAGAAGTAGTGTGGAAG+AGG 0.407282 2.2:-15554899 MS.gene059857:CDS
CCACAAGTGGCTAGGTTCCC+TGG 0.408466 2.2:-15554636 MS.gene059857:CDS
GTTATAACTTCATCCACAAT+TGG 0.426097 2.2:+15555492 None:intergenic
GTCAGATTCACTTCCTTCAC+TGG 0.429115 2.2:+15555266 None:intergenic
CTTTCTCTGTACAGATTGAC+AGG 0.431846 2.2:-15554819 MS.gene059857:CDS
TCATTCTTAAACAAGCATAT+CGG 0.434369 2.2:-15555649 MS.gene059857:CDS
ATTCTGCTTCAGCAAATTCA+AGG 0.460631 2.2:-15556704 MS.gene059857:intron
GACCTTCGAATAAGGAATAA+GGG 0.464630 2.2:+15554697 None:intergenic
TCTGCCATACAATAACTCAT+AGG 0.466009 2.2:+15555693 None:intergenic
GTGTTTGGTAAGATAGAGAA+AGG 0.473304 2.2:+15557347 None:intergenic
TCCTTATTCGAAGGTCTTGG+AGG 0.474374 2.2:-15554690 MS.gene059857:CDS
AAATACTGGGGTGCTGCTCA+TGG 0.483810 2.2:-15555408 MS.gene059857:CDS
CGCCCTTATTCCTTATTCGA+AGG 0.485112 2.2:-15554699 MS.gene059857:CDS
TTTATGCTTGAAGATTGTTA+AGG 0.488334 2.2:-15556733 MS.gene059857:CDS
GAGGTGAGAGAGTGTGGTGT+TGG 0.490011 2.2:+15557373 None:intergenic
ATGCCTTACCGTGTAGGTAG+TGG 0.491158 2.2:+15555612 None:intergenic
AGGGGTCTTCTTAAGCGAGT+TGG 0.504459 2.2:-15554879 MS.gene059857:CDS
GAGTACTTGTACAGGGCTAA+AGG 0.505457 2.2:-15554789 MS.gene059857:CDS
TCGCAAGCAGCGGAAGATGC+AGG 0.507394 2.2:-15554918 MS.gene059857:CDS
AGTGTTGTCTCTGGCATGAA+TGG 0.507407 2.2:+15557433 None:intergenic
TTGCCCTATGAGTTATTGTA+TGG 0.507703 2.2:-15555697 MS.gene059857:CDS
AGGAGTGAGGTGAGAGAGTG+TGG 0.508542 2.2:+15557367 None:intergenic
GTGACTCAAAGTTCATAGCC+AGG 0.511069 2.2:+15554618 None:intergenic
CAAGAGCACAAACACCGGCA+CGG 0.514774 2.2:+15555916 None:intergenic
TGGATCAATCATGTCTAGAA+AGG 0.516656 2.2:+15554656 None:intergenic
GAAACAAGGAGTACTTGTAC+AGG 0.518932 2.2:-15554797 MS.gene059857:CDS
ACGTTCATCTGTGGCCGTGC+CGG 0.520688 2.2:-15555930 MS.gene059857:CDS
ATGAATGGTGGCAAGTTGTT+CGG 0.523714 2.2:+15557448 None:intergenic
GTGGATGAAGTTATAACAGC+TGG 0.524603 2.2:-15555486 MS.gene059857:CDS
TGGTGTTGGTGTTTGAGAAG+AGG 0.528091 2.2:+15557387 None:intergenic
TGGCAGAACAGGTTACTTGT+GGG 0.530458 2.2:-15555677 MS.gene059857:CDS
CTGCCATACAATAACTCATA+GGG 0.530788 2.2:+15555694 None:intergenic
ATTCGAAGGTCTTGGAGGAA+TGG 0.532742 2.2:-15554685 MS.gene059857:CDS
GGAATTTGCCACGGCATCAG+TGG 0.534765 2.2:-15554858 MS.gene059857:CDS
GGCAATTATCCCTCCAGTGA+AGG 0.536493 2.2:-15555279 MS.gene059857:CDS
GATGTCCTTTGATGTATGAA+TGG 0.539425 2.2:-15555440 MS.gene059857:CDS
AGCACCAAGAGCACAAACAC+CGG 0.540687 2.2:+15555911 None:intergenic
TGGTAGAAAATTAGCTCACA+TGG 0.549599 2.2:-15555466 MS.gene059857:CDS
CATCTTGTGCATTGGTGTCA+CGG 0.556078 2.2:-15555243 MS.gene059857:CDS
AGATGAGGTGGAAGATGTCA+AGG 0.556890 2.2:-15555340 MS.gene059857:CDS
AGTGAGACATGGGGTTCTAG+TGG 0.556968 2.2:-15556855 MS.gene059857:CDS
GTAAGATAGAGAAAGGAGTG+AGG 0.560989 2.2:+15557354 None:intergenic
CCAGGGAACCTAGCCACTTG+TGG 0.562759 2.2:+15554636 None:intergenic
TTAAGGTGGTGAGTGAGACA+TGG 0.563607 2.2:-15556866 MS.gene059857:intron
TAAGGTGGTGAGTGAGACAT+GGG 0.563863 2.2:-15556865 MS.gene059857:intron
GCGCAAGAATTGATCGGCCA+AGG 0.565355 2.2:-15554741 MS.gene059857:CDS
ATGGCAGAACAGGTTACTTG+TGG 0.565602 2.2:-15555678 MS.gene059857:CDS
AACTCATAGGGCAAATCAGT+GGG 0.573329 2.2:+15555706 None:intergenic
GAAGGACTATACGCTTTACA+CGG 0.574930 2.2:-15556823 MS.gene059857:CDS
AGGAGAAGTAGTGTGGAAGA+GGG 0.575038 2.2:-15554898 MS.gene059857:CDS
GAATTTGCCACGGCATCAGT+GGG 0.580597 2.2:-15554857 MS.gene059857:CDS
TGCCTTACCGTGTAGGTAGT+GGG 0.583545 2.2:+15555613 None:intergenic
GTACAGAGAAAGAAAGACAT+AGG 0.584385 2.2:+15554830 None:intergenic
ATCGGCCAAGGAAAGATGCA+TGG 0.587677 2.2:-15554729 MS.gene059857:CDS
AGATTCACTTCCTTCACTGG+AGG 0.588226 2.2:+15555269 None:intergenic
GCTGTTATAGCTAAGCATGC+TGG 0.588262 2.2:-15555891 MS.gene059857:CDS
GAAACTTAAGCCAGATGAGG+TGG 0.590092 2.2:-15555352 MS.gene059857:CDS
TGATTGATCCACAAGTGGCT+AGG 0.590458 2.2:-15554644 MS.gene059857:CDS
CACAATACCCACTACCTACA+CGG 0.590900 2.2:-15555620 MS.gene059857:intron
CGTGACACCAATGCACAAGA+TGG 0.593365 2.2:+15555244 None:intergenic
GATAGAGCGCAAGAATTGAT+CGG 0.594878 2.2:-15554747 MS.gene059857:CDS
GTACAGATTGACAGGAAACA+AGG 0.596022 2.2:-15554811 MS.gene059857:CDS
ACAAGCGTAAGAGTTACTCC+AGG 0.600044 2.2:+15555216 None:intergenic
ATGGCATGGGAAGAAATACT+GGG 0.602526 2.2:-15555421 MS.gene059857:CDS
CCATGCCATTCATACATCAA+AGG 0.604422 2.2:+15555435 None:intergenic
CTACCAAGTAACCAAGAATG+TGG 0.605333 2.2:-15556766 MS.gene059857:CDS
AAGGACTATACGCTTTACAC+GGG 0.607848 2.2:-15556822 MS.gene059857:CDS
AAGCGAGTTGGAATTTGCCA+CGG 0.608188 2.2:-15554867 MS.gene059857:CDS
GAACATATGCCTTACCGTGT+AGG 0.609489 2.2:+15555606 None:intergenic
GATTCACTTCCTTCACTGGA+GGG 0.611653 2.2:+15555270 None:intergenic
AGGTGTTCCCACTGATGCCG+TGG 0.612064 2.2:+15554850 None:intergenic
TTTAGCCGGAGATGTCTGAT+CGG 0.615895 2.2:-15557477 None:intergenic
GGAGAAGTAGTGTGGAAGAG+GGG 0.618091 2.2:-15554897 MS.gene059857:CDS
TTCTAGTGGGAACCGTGTGA+AGG 0.618170 2.2:-15556841 MS.gene059857:CDS
GTGAGACATGGGGTTCTAGT+GGG 0.619681 2.2:-15556854 MS.gene059857:CDS
TTACCTTAGACAATTCAAAG+AGG 0.627912 2.2:-15555847 MS.gene059857:intron
TAACTCATAGGGCAAATCAG+TGG 0.629793 2.2:+15555705 None:intergenic
CTTTGATGTATGAATGGCAT+GGG 0.630619 2.2:-15555434 MS.gene059857:CDS
AGACATGATTGATCCACAAG+TGG 0.632392 2.2:-15554649 MS.gene059857:CDS
TGACTCAAAGTTCATAGCCA+GGG 0.635792 2.2:+15554619 None:intergenic
AGGACTATACGCTTTACACG+GGG 0.636276 2.2:-15556821 MS.gene059857:CDS
AAGATGCAGGAGAAGTAGTG+TGG 0.637341 2.2:-15554905 MS.gene059857:CDS
GGCCACAGATGAACGTCACA+CGG 0.640066 2.2:+15555937 None:intergenic
TACGCTTGTAAAAGCAGCCG+AGG 0.641247 2.2:-15555202 MS.gene059857:intron
AATCTGACCATCTTGTGCAT+TGG 0.646088 2.2:-15555251 MS.gene059857:CDS
CATGAAACTTAAGCCAGATG+AGG 0.673066 2.2:-15555355 MS.gene059857:CDS
GTTGTCTCTGGCATGAATGG+TGG 0.674768 2.2:+15557436 None:intergenic
AGCCGTGTGACGTTCATCTG+TGG 0.685914 2.2:-15555939 MS.gene059857:CDS
CCTTTGATGTATGAATGGCA+TGG 0.688871 2.2:-15555435 MS.gene059857:CDS
GATGAGGTGGAAGATGTCAA+GGG 0.689281 2.2:-15555339 MS.gene059857:CDS
AAACAAGGAGTACTTGTACA+GGG 0.691885 2.2:-15554796 MS.gene059857:CDS
GGTAGAAAATTAGCTCACAT+GGG 0.694372 2.2:-15555465 MS.gene059857:CDS
GGACTATACGCTTTACACGG+GGG 0.714635 2.2:-15556820 MS.gene059857:CDS
AAGGTGGTGAGTGAGACATG+GGG 0.727439 2.2:-15556864 MS.gene059857:CDS
GGCCAAGGAAAGATGCATGG+AGG 0.732329 2.2:-15554726 MS.gene059857:CDS
AAAGCGTATAGTCCTTCACA+CGG 0.761639 2.2:+15556829 None:intergenic
TGGCATGGGAAGAAATACTG+GGG 0.762911 2.2:-15555420 MS.gene059857:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTCATTATAAAAAAAAATAA+AGG + chr2.2:15556922-15556941 None:intergenic 10.0%
!!! CTTTATTTTTTTTTATAATG+AGG - chr2.2:15556920-15556939 MS.gene059857:intron 10.0%
!!! AAATTTTCATTTTTTCTTTC+TGG - chr2.2:15554820-15554839 MS.gene059857:CDS 15.0%
!!! AATTTTCATTTTTTCTTTCT+GGG - chr2.2:15554821-15554840 MS.gene059857:CDS 15.0%
!!! AGAAAAAATGAAAATTTTTG+TGG + chr2.2:15554817-15554836 None:intergenic 15.0%
!!! TTGTTTATGTATTACTTTTA+AGG - chr2.2:15555206-15555225 MS.gene059857:CDS 15.0%
!! AAACTTTGATTGTTCAATTT+AGG + chr2.2:15556065-15556084 None:intergenic 20.0%
!! CTAATTTCTTTACCATAATT+AGG - chr2.2:15557128-15557147 MS.gene059857:intron 20.0%
!! TATTAATTACGTTAAACAGA+TGG + chr2.2:15555743-15555762 None:intergenic 20.0%
!! TTCATCATAAACCATAAATT+TGG + chr2.2:15555695-15555714 None:intergenic 20.0%
!!! AAATTAAAAGCCAATTTTCA+TGG + chr2.2:15556562-15556581 None:intergenic 20.0%
!!! TGTTAATATATTTTGTTGTC+TGG - chr2.2:15555947-15555966 MS.gene059857:intron 20.0%
!!! TTATGATGAATTTTGAAACA+TGG - chr2.2:15555705-15555724 MS.gene059857:CDS 20.0%
!!! TTTACCATAATTAGGTTTTT+GGG - chr2.2:15557136-15557155 MS.gene059857:intron 20.0%
!!! TTTATGTATTACTTTTAAGG+TGG - chr2.2:15555209-15555228 MS.gene059857:CDS 20.0%
! AATAAAGGGAAATATTACCT+CGG + chr2.2:15556907-15556926 None:intergenic 25.0%
! AATGCAATTTAGATGGTTTA+TGG - chr2.2:15555530-15555549 MS.gene059857:intron 25.0%
! AGTTGAACATCTGAAAATAA+TGG + chr2.2:15555631-15555650 None:intergenic 25.0%
! GATTTCTGTACAATTAAATG+TGG - chr2.2:15555477-15555496 MS.gene059857:CDS 25.0%
! TAATGAAGTTACATTGGTAT+TGG + chr2.2:15555585-15555604 None:intergenic 25.0%
! TCACATAAATGCTTATGTAT+AGG + chr2.2:15555155-15555174 None:intergenic 25.0%
! TCATTCTTAAACAAGCATAT+CGG - chr2.2:15556440-15556459 MS.gene059857:intron 25.0%
! TCCAATTTACACATGAATTT+CGG - chr2.2:15556306-15556325 MS.gene059857:intron 25.0%
! TGCAAAAATGCAATTCAATT+AGG + chr2.2:15554974-15554993 None:intergenic 25.0%
! TTTATCCTTAGGATTAAGTT+AGG - chr2.2:15555997-15556016 MS.gene059857:intron 25.0%
! TTTATGCTTGAAGATTGTTA+AGG - chr2.2:15555356-15555375 MS.gene059857:CDS 25.0%
!! CTTTACCATAATTAGGTTTT+TGG - chr2.2:15557135-15557154 MS.gene059857:intron 25.0%
!! TGTGTTTTCTAATTCTTCTT+AGG - chr2.2:15554948-15554967 MS.gene059857:intron 25.0%
!! TTACCATAATTAGGTTTTTG+GGG - chr2.2:15557137-15557156 MS.gene059857:intron 25.0%
!!! GTTAAACTTCTTTTTCGTAT+TGG - chr2.2:15556331-15556350 MS.gene059857:intron 25.0%
!!! TCTGTTTTTTCATTTTCGTT+AGG - chr2.2:15554856-15554875 MS.gene059857:CDS 25.0%
AGATGGTTTATGGATATATC+AGG - chr2.2:15555540-15555559 MS.gene059857:intron 30.0%
AGTGGTTAATGCAATTTAGA+TGG - chr2.2:15555523-15555542 MS.gene059857:intron 30.0%
ATACCTCTTTGAATTGTCTA+AGG + chr2.2:15556248-15556267 None:intergenic 30.0%
ATTAAAAATCGTTTCTCGAG+TGG - chr2.2:15556789-15556808 MS.gene059857:CDS 30.0%
ATTAACCACAAATTTGAACG+TGG - chr2.2:15555601-15555620 MS.gene059857:intron 30.0%
ATTTGAACACCTGGAAATTA+TGG - chr2.2:15555659-15555678 MS.gene059857:CDS 30.0%
CATTTCCTAACTTAATCCTA+AGG + chr2.2:15556005-15556024 None:intergenic 30.0%
CCAATTTACACATGAATTTC+GGG - chr2.2:15556307-15556326 MS.gene059857:intron 30.0%
CTTAAAGACATGTCATAAGT+TGG + chr2.2:15555027-15555046 None:intergenic 30.0%
CTTAGGATTAAGTTAGGAAA+TGG - chr2.2:15556003-15556022 MS.gene059857:intron 30.0%
GTCAATGACATCCAAATTTA+TGG - chr2.2:15555681-15555700 MS.gene059857:CDS 30.0%
GTGAAGCTTGTATTTGAATA+TGG + chr2.2:15556964-15556983 None:intergenic 30.0%
GTTATAACTTCATCCACAAT+TGG + chr2.2:15556600-15556619 None:intergenic 30.0%
TACGTTAAACAGATGGTTTA+TGG + chr2.2:15555736-15555755 None:intergenic 30.0%
TATCCCCAAAAACCTAATTA+TGG + chr2.2:15557143-15557162 None:intergenic 30.0%
TCAGGTTTGTGCAAATTTAT+TGG + chr2.2:15557012-15557031 None:intergenic 30.0%
TGTGGTTAATGAAGTTACAT+TGG + chr2.2:15555591-15555610 None:intergenic 30.0%
TTACCTTAGACAATTCAAAG+AGG - chr2.2:15556242-15556261 MS.gene059857:intron 30.0%
! AGGAATTATGAACGTTTTGA+TGG - chr2.2:15556710-15556729 MS.gene059857:CDS 30.0%
! TTTAAAACTACGCCTTACAA+CGG + chr2.2:15555449-15555468 None:intergenic 30.0%
!! ATAATTAGGTTTTTGGGGAT+AGG - chr2.2:15557142-15557161 MS.gene059857:intron 30.0%
!! TAATTAGGTTTTTGGGGATA+GGG - chr2.2:15557143-15557162 MS.gene059857:intron 30.0%
!!! CGTATTGGTGCTTTTATTTT+TGG - chr2.2:15556346-15556365 MS.gene059857:intron 30.0%
AAGGCATATGTTCTTCATCA+TGG - chr2.2:15556493-15556512 MS.gene059857:intron 35.0%
ACTAAGATCATCCACATTCT+TGG + chr2.2:15555337-15555356 None:intergenic 35.0%
AGACCTTCGAATAAGGAATA+AGG + chr2.2:15557396-15557415 None:intergenic 35.0%
AGCACTTCACTTCAAACAAT+TGG - chr2.2:15554787-15554806 MS.gene059857:CDS 35.0%
ATCAAAGTTTCAGCCTGAAA+TGG - chr2.2:15556075-15556094 MS.gene059857:intron 35.0%
ATTCTGCTTCAGCAAATTCA+AGG - chr2.2:15555385-15555404 MS.gene059857:CDS 35.0%
ATTTGCTCGTGTTTATCCTT+AGG - chr2.2:15555986-15556005 MS.gene059857:intron 35.0%
CAACGAAATTTAGACAGCTT+TGG + chr2.2:15555804-15555823 None:intergenic 35.0%
CAAGTTAGAAACTTTCACGT+TGG - chr2.2:15554881-15554900 MS.gene059857:CDS 35.0%
CCCGAAATTCATGTGTAAAT+TGG + chr2.2:15556310-15556329 None:intergenic 35.0%
CTGCAAAATCCATTGAAATC+AGG + chr2.2:15557030-15557049 None:intergenic 35.0%
CTGCCATACAATAACTCATA+GGG + chr2.2:15556398-15556417 None:intergenic 35.0%
CTTTGATGTATGAATGGCAT+GGG - chr2.2:15556655-15556674 MS.gene059857:intron 35.0%
GACCTTCGAATAAGGAATAA+GGG + chr2.2:15557395-15557414 None:intergenic 35.0%
GATGTCCTTTGATGTATGAA+TGG - chr2.2:15556649-15556668 MS.gene059857:intron 35.0%
GGTAGAAAATTAGCTCACAT+GGG - chr2.2:15556624-15556643 MS.gene059857:intron 35.0%
GTACAGAGAAAGAAAGACAT+AGG + chr2.2:15557262-15557281 None:intergenic 35.0%
GTGTTTGGTAAGATAGAGAA+AGG + chr2.2:15554745-15554764 None:intergenic 35.0%
GTTAACCACGTTCAAATTTG+TGG + chr2.2:15555609-15555628 None:intergenic 35.0%
TACTCTCTATTTCATGCATC+TGG - chr2.2:15555408-15555427 MS.gene059857:CDS 35.0%
TATTCCTTATTCGAAGGTCT+TGG - chr2.2:15557396-15557415 MS.gene059857:CDS 35.0%
TCAAAGTTTCAGCCTGAAAT+GGG - chr2.2:15556076-15556095 MS.gene059857:intron 35.0%
TCTGCCATACAATAACTCAT+AGG + chr2.2:15556399-15556418 None:intergenic 35.0%
TGGATCAATCATGTCTAGAA+AGG + chr2.2:15557436-15557455 None:intergenic 35.0%
TGGTAGAAAATTAGCTCACA+TGG - chr2.2:15556623-15556642 MS.gene059857:intron 35.0%
TGTCATTGACCATAATTTCC+AGG + chr2.2:15555671-15555690 None:intergenic 35.0%
TTAAATGTGGACAACTTTCG+CGG - chr2.2:15555490-15555509 MS.gene059857:CDS 35.0%
TTGCACAAACCTGATTTCAA+TGG - chr2.2:15557018-15557037 MS.gene059857:intron 35.0%
TTGCCCTATGAGTTATTGTA+TGG - chr2.2:15556392-15556411 MS.gene059857:intron 35.0%
TTGGCTACAACTTCTATAAG+TGG + chr2.2:15555785-15555804 None:intergenic 35.0%
TTTCACGTTGGTGCAATAAT+GGG - chr2.2:15554893-15554912 MS.gene059857:CDS 35.0%
TTTGCAGTGTTCATTGTATG+TGG - chr2.2:15557043-15557062 MS.gene059857:intron 35.0%
! TCCTTTACATCACATTGACT+CGG + chr2.2:15556988-15557007 None:intergenic 35.0%
! TGAAGTTACATTGGTATTGG+TGG + chr2.2:15555582-15555601 None:intergenic 35.0%
! TTTTTGACAGAGACCAATTG+TGG - chr2.2:15556584-15556603 MS.gene059857:intron 35.0%
!! AAACAAGGAGTACTTGTACA+GGG - chr2.2:15557293-15557312 MS.gene059857:intron 35.0%
!! AACGGCATACTTGGTTTTTA+AGG - chr2.2:15555299-15555318 MS.gene059857:CDS 35.0%
!! TGCTGTAACTTGTAGTTTTG+AGG + chr2.2:15554771-15554790 None:intergenic 35.0%
!! TTTCGCGGTAACTTTTTAAG+TGG - chr2.2:15555505-15555524 MS.gene059857:intron 35.0%
AAAGCGTATAGTCCTTCACA+CGG + chr2.2:15555263-15555282 None:intergenic 40.0%
AACTCATAGGGCAAATCAGT+GGG + chr2.2:15556386-15556405 None:intergenic 40.0%
AAGGACTATACGCTTTACAC+GGG - chr2.2:15555267-15555286 MS.gene059857:CDS 40.0%
AATCTGACCATCTTGTGCAT+TGG - chr2.2:15556838-15556857 MS.gene059857:CDS 40.0%
AATGGCATGGGAAGAAATAC+TGG - chr2.2:15556667-15556686 MS.gene059857:intron 40.0%
AGACATGATTGATCCACAAG+TGG - chr2.2:15557440-15557459 MS.gene059857:CDS 40.0%
ATGAATGGTGGCAAGTTGTT+CGG + chr2.2:15554644-15554663 None:intergenic 40.0%
ATGGCATGGGAAGAAATACT+GGG - chr2.2:15556668-15556687 MS.gene059857:intron 40.0%
CATCCACATTCTTGGTTACT+TGG + chr2.2:15555329-15555348 None:intergenic 40.0%
CATGAAACTTAAGCCAGATG+AGG - chr2.2:15556734-15556753 MS.gene059857:CDS 40.0%
CCATGCCATTCATACATCAA+AGG + chr2.2:15556657-15556676 None:intergenic 40.0%
CCTTTGATGTATGAATGGCA+TGG - chr2.2:15556654-15556673 MS.gene059857:intron 40.0%
CTACCAAGTAACCAAGAATG+TGG - chr2.2:15555323-15555342 MS.gene059857:CDS 40.0%
CTTTCACGTTGGTGCAATAA+TGG - chr2.2:15554892-15554911 MS.gene059857:CDS 40.0%
CTTTCTCTGTACAGATTGAC+AGG - chr2.2:15557270-15557289 MS.gene059857:intron 40.0%
GAAGGACTATACGCTTTACA+CGG - chr2.2:15555266-15555285 MS.gene059857:CDS 40.0%
GACTTAACTTAGCCGTTGTA+AGG - chr2.2:15555434-15555453 MS.gene059857:CDS 40.0%
GAGTTATTGTATGGCAGAAC+AGG - chr2.2:15556401-15556420 MS.gene059857:intron 40.0%
GATAGAGCGCAAGAATTGAT+CGG - chr2.2:15557342-15557361 MS.gene059857:CDS 40.0%
GTAAGATAGAGAAAGGAGTG+AGG + chr2.2:15554738-15554757 None:intergenic 40.0%
GTACAGATTGACAGGAAACA+AGG - chr2.2:15557278-15557297 MS.gene059857:intron 40.0%
GTGGATGAAGTTATAACAGC+TGG - chr2.2:15556603-15556622 MS.gene059857:intron 40.0%
GTGGTTAATGCACTTCGAAT+AGG + chr2.2:15555766-15555785 None:intergenic 40.0%
TAACTCATAGGGCAAATCAG+TGG + chr2.2:15556387-15556406 None:intergenic 40.0%
TCCAGGTGTTCAAATCTACA+TGG + chr2.2:15555654-15555673 None:intergenic 40.0%
TCCATGTAGATTTGAACACC+TGG - chr2.2:15555650-15555669 MS.gene059857:CDS 40.0%
!! GAAACAAGGAGTACTTGTAC+AGG - chr2.2:15557292-15557311 MS.gene059857:intron 40.0%
!! TGTCGACTTGCCATGAAAAT+TGG - chr2.2:15556549-15556568 MS.gene059857:intron 40.0%
!!! GTAGTTTTGAGGTGAGTGTT+TGG + chr2.2:15554760-15554779 None:intergenic 40.0%
AAATGGGAGGATGGGAAGTA+AGG - chr2.2:15556092-15556111 MS.gene059857:intron 45.0%
AAGATGCAGGAGAAGTAGTG+TGG - chr2.2:15557184-15557203 MS.gene059857:intron 45.0%
AAGTTTCAGCCTGAAATGGG+AGG - chr2.2:15556079-15556098 MS.gene059857:intron 45.0%
ACAAGCGTAAGAGTTACTCC+AGG + chr2.2:15556876-15556895 None:intergenic 45.0%
AGATGAGGTGGAAGATGTCA+AGG - chr2.2:15556749-15556768 MS.gene059857:CDS 45.0%
AGATTCACTTCCTTCACTGG+AGG + chr2.2:15556823-15556842 None:intergenic 45.0%
AGGACTATACGCTTTACACG+GGG - chr2.2:15555268-15555287 MS.gene059857:CDS 45.0%
AGGAGAAGTAGTGTGGAAGA+GGG - chr2.2:15557191-15557210 MS.gene059857:intron 45.0%
AGTGTTGTCTCTGGCATGAA+TGG + chr2.2:15554659-15554678 None:intergenic 45.0%
ATGGCAGAACAGGTTACTTG+TGG - chr2.2:15556411-15556430 MS.gene059857:intron 45.0%
ATTCGAAGGTCTTGGAGGAA+TGG - chr2.2:15557404-15557423 MS.gene059857:CDS 45.0%
CACAATACCCACTACCTACA+CGG - chr2.2:15556469-15556488 MS.gene059857:intron 45.0%
CGCCCTTATTCCTTATTCGA+AGG - chr2.2:15557390-15557409 MS.gene059857:CDS 45.0%
GAAACTTAAGCCAGATGAGG+TGG - chr2.2:15556737-15556756 MS.gene059857:CDS 45.0%
GAACATATGCCTTACCGTGT+AGG + chr2.2:15556486-15556505 None:intergenic 45.0%
GAGTACTTGTACAGGGCTAA+AGG - chr2.2:15557300-15557319 MS.gene059857:intron 45.0%
GATGAGGTGGAAGATGTCAA+GGG - chr2.2:15556750-15556769 MS.gene059857:CDS 45.0%
GATTCACTTCCTTCACTGGA+GGG + chr2.2:15556822-15556841 None:intergenic 45.0%
GCCGAGTCAATGTGATGTAA+AGG - chr2.2:15556984-15557003 MS.gene059857:intron 45.0%
GCTGTTATAGCTAAGCATGC+TGG - chr2.2:15556198-15556217 MS.gene059857:intron 45.0%
GTCAGATTCACTTCCTTCAC+TGG + chr2.2:15556826-15556845 None:intergenic 45.0%
TAAGGTGGTGAGTGAGACAT+GGG - chr2.2:15555224-15555243 MS.gene059857:CDS 45.0%
TCCTCCAAGACCTTCGAATA+AGG + chr2.2:15557403-15557422 None:intergenic 45.0%
TCCTTATTCGAAGGTCTTGG+AGG - chr2.2:15557399-15557418 MS.gene059857:CDS 45.0%
TGATTGATCCACAAGTGGCT+AGG - chr2.2:15557445-15557464 MS.gene059857:CDS 45.0%
TGGCAGAACAGGTTACTTGT+GGG - chr2.2:15556412-15556431 MS.gene059857:intron 45.0%
TGGCATGGGAAGAAATACTG+GGG - chr2.2:15556669-15556688 MS.gene059857:intron 45.0%
TTAAGGTGGTGAGTGAGACA+TGG - chr2.2:15555223-15555242 MS.gene059857:CDS 45.0%
TTGACATCTTCCACCTCATC+TGG + chr2.2:15556750-15556769 None:intergenic 45.0%
! AAGCGAGTTGGAATTTGCCA+CGG - chr2.2:15557222-15557241 MS.gene059857:intron 45.0%
! GCTTCTTCAAGTGTTGTCTC+TGG + chr2.2:15554668-15554687 None:intergenic 45.0%
! TAGGGAATTTTCGCAAGCAG+CGG - chr2.2:15557161-15557180 MS.gene059857:intron 45.0%
!! ACGTTGGTGCAATAATGGGT+CGG - chr2.2:15554897-15554916 MS.gene059857:CDS 45.0%
!! CATCTTGTGCATTGGTGTCA+CGG - chr2.2:15556846-15556865 MS.gene059857:CDS 45.0%
!! TGGTGTTGGTGTTTGAGAAG+AGG + chr2.2:15554705-15554724 None:intergenic 45.0%
!! TCATTATAAAAAAAAATAAA+GGG + chr2.2:15556921-15556940 None:intergenic 5.0%
AAGGTGGTGAGTGAGACATG+GGG - chr2.2:15555225-15555244 MS.gene059857:CDS 50.0%
ACTTCCCATCCTCCCATTTC+AGG + chr2.2:15556091-15556110 None:intergenic 50.0%
AGGGGTCTTCTTAAGCGAGT+TGG - chr2.2:15557210-15557229 MS.gene059857:intron 50.0%
ATCGGCCAAGGAAAGATGCA+TGG - chr2.2:15557360-15557379 MS.gene059857:CDS 50.0%
ATGCCTTACCGTGTAGGTAG+TGG + chr2.2:15556480-15556499 None:intergenic 50.0%
CAGGAGAAGTAGTGTGGAAG+AGG - chr2.2:15557190-15557209 MS.gene059857:intron 50.0%
CGTGACACCAATGCACAAGA+TGG + chr2.2:15556848-15556867 None:intergenic 50.0%
CTCCTCCATGCATCTTTCCT+TGG + chr2.2:15557368-15557387 None:intergenic 50.0%
GAATTTGCCACGGCATCAGT+GGG - chr2.2:15557232-15557251 MS.gene059857:intron 50.0%
GGACTATACGCTTTACACGG+GGG - chr2.2:15555269-15555288 MS.gene059857:CDS 50.0%
GGAGAAGTAGTGTGGAAGAG+GGG - chr2.2:15557192-15557211 MS.gene059857:intron 50.0%
GGCAATTATCCCTCCAGTGA+AGG - chr2.2:15556810-15556829 MS.gene059857:CDS 50.0%
GGTTCTTGGAACGGCATACT+TGG - chr2.2:15555290-15555309 MS.gene059857:CDS 50.0%
GTTGTCTCTGGCATGAATGG+TGG + chr2.2:15554656-15554675 None:intergenic 50.0%
TACCCACTACCTACACGGTA+AGG - chr2.2:15556474-15556493 MS.gene059857:intron 50.0%
TACGCTTGTAAAAGCAGCCG+AGG - chr2.2:15556887-15556906 MS.gene059857:intron 50.0%
TCAGCCTGAAATGGGAGGAT+GGG - chr2.2:15556084-15556103 MS.gene059857:intron 50.0%
TGCCTTACCGTGTAGGTAGT+GGG + chr2.2:15556479-15556498 None:intergenic 50.0%
TTACACGGGGGTTCTTGGAA+CGG - chr2.2:15555281-15555300 MS.gene059857:CDS 50.0%
TTCAGCCTGAAATGGGAGGA+TGG - chr2.2:15556083-15556102 MS.gene059857:intron 50.0%
TTCTAGTGGGAACCGTGTGA+AGG - chr2.2:15555248-15555267 MS.gene059857:CDS 50.0%
! AGCACCAAGAGCACAAACAC+CGG + chr2.2:15556181-15556200 None:intergenic 50.0%
! AGTGAGACATGGGGTTCTAG+TGG - chr2.2:15555234-15555253 MS.gene059857:CDS 50.0%
! GTGAGACATGGGGTTCTAGT+GGG - chr2.2:15555235-15555254 MS.gene059857:CDS 50.0%
!! AAATACTGGGGTGCTGCTCA+TGG - chr2.2:15556681-15556700 MS.gene059857:intron 50.0%
ACGCTTTACACGGGGGTTCT+TGG - chr2.2:15555276-15555295 MS.gene059857:CDS 55.0%
AGCCGTGTGACGTTCATCTG+TGG - chr2.2:15556150-15556169 MS.gene059857:intron 55.0%
AGGAGTGAGGTGAGAGAGTG+TGG + chr2.2:15554725-15554744 None:intergenic 55.0%
GCGCAAGAATTGATCGGCCA+AGG - chr2.2:15557348-15557367 MS.gene059857:CDS 55.0%
GGAATTTGCCACGGCATCAG+TGG - chr2.2:15557231-15557250 MS.gene059857:intron 55.0%
GGCCAAGGAAAGATGCATGG+AGG - chr2.2:15557363-15557382 MS.gene059857:CDS 55.0%
GGCCACAGATGAACGTCACA+CGG + chr2.2:15556155-15556174 None:intergenic 55.0%
! CAAGAGCACAAACACCGGCA+CGG + chr2.2:15556176-15556195 None:intergenic 55.0%
! GAGGTGAGAGAGTGTGGTGT+TGG + chr2.2:15554719-15554738 None:intergenic 55.0%
ACGTTCATCTGTGGCCGTGC+CGG - chr2.2:15556159-15556178 MS.gene059857:intron 60.0%
AGGTGTTCCCACTGATGCCG+TGG + chr2.2:15557242-15557261 None:intergenic 60.0%
CCACAAGTGGCTAGGTTCCC+TGG - chr2.2:15557453-15557472 MS.gene059857:CDS 60.0%
CCAGGGAACCTAGCCACTTG+TGG + chr2.2:15557456-15557475 None:intergenic 60.0%
TCGCAAGCAGCGGAAGATGC+AGG - chr2.2:15557171-15557190 MS.gene059857:intron 60.0%
! CGTGCCGGTGTTTGTGCTCT+TGG - chr2.2:15556174-15556193 MS.gene059857:intron 60.0%
!! CATTGGTGTCACGGTGCTCC+TGG - chr2.2:15556855-15556874 MS.gene059857:CDS 60.0%
!! GGTGCTGCTCATGGACTTGC+AGG - chr2.2:15556690-15556709 MS.gene059857:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 15554623 15557488 15554623 ID=MS.gene059857
chr2.2 mRNA 15554623 15557488 15554623 ID=MS.gene059857.t1;Parent=MS.gene059857
chr2.2 exon 15557303 15557488 15557303 ID=MS.gene059857.t1.exon1;Parent=MS.gene059857.t1
chr2.2 CDS 15557303 15557488 15557303 ID=cds.MS.gene059857.t1;Parent=MS.gene059857.t1
chr2.2 exon 15556705 15556883 15556705 ID=MS.gene059857.t1.exon2;Parent=MS.gene059857.t1
chr2.2 CDS 15556705 15556883 15556705 ID=cds.MS.gene059857.t1;Parent=MS.gene059857.t1
chr2.2 exon 15555848 15555959 15555848 ID=MS.gene059857.t1.exon3;Parent=MS.gene059857.t1
chr2.2 CDS 15555848 15555959 15555848 ID=cds.MS.gene059857.t1;Parent=MS.gene059857.t1
chr2.2 exon 15555621 15555737 15555621 ID=MS.gene059857.t1.exon4;Parent=MS.gene059857.t1
chr2.2 CDS 15555621 15555737 15555621 ID=cds.MS.gene059857.t1;Parent=MS.gene059857.t1
chr2.2 exon 15555203 15555517 15555203 ID=MS.gene059857.t1.exon5;Parent=MS.gene059857.t1
chr2.2 CDS 15555203 15555517 15555203 ID=cds.MS.gene059857.t1;Parent=MS.gene059857.t1
chr2.2 exon 15554623 15554961 15554623 ID=MS.gene059857.t1.exon6;Parent=MS.gene059857.t1
chr2.2 CDS 15554623 15554961 15554623 ID=cds.MS.gene059857.t1;Parent=MS.gene059857.t1
Gene Sequence

>MS.gene059857

ATGTCTGATCGGTTTTTTCCGAACAACTTGCCACCATTCATGCCAGAGACAACACTTGAAGAAGCAGAAGCAGAAGCAACCTCTTCTCAAACACCAACACCACACTCTCTCACCTCACTCCTTTCTCTATCTTACCAAACACTCACCTCAAAACTACAAGTTACAGCACTTCACTTCAAACAATTGGTACTCCACAAAAATTTTCATTTTTTCTTTCTGGGTTTCTTTCAATTTCTGTTTTTTCATTTTCGTTAGGTTCAAGTTAGAAACTTTCACGTTGGTGCAATAATGGGTCGGTGTTTATTTGCTCACAAAGTGTTTGTGCTGTGTTTTCTAATTCTTCTTAGGCCTAATTGAATTGCATTTTTGCATTTATCTACTATTTGAAAGAGCTTATGAAACCAACTTATGACATGTCTTTAAGCTGTTTTTAGCTTATTTTCATACTCGCATGACTTATGAAAACAATTCATAACTTATATGAAGTATAAATAGTTTGAATTTATTGTATCTTTTGTTAAAGAAATAACCTATACATAAGCATTTATGTGATAGACGCTTATCTTATAAGTATTTAACTAAGTTGTTTATGTATTACTTTTAAGGTGGTGAGTGAGACATGGGGTTCTAGTGGGAACCGTGTGAAGGACTATACGCTTTACACGGGGGTTCTTGGAACGGCATACTTGGTTTTTAAGGTCTACCAAGTAACCAAGAATGTGGATGATCTTAGTTTATGCTTGAAGATTGTTAAGGCTTGTGATTCTGCTTCAGCAAATTCAAGGTACTCTCTATTTCATGCATCTGGTAAGACTTAACTTAGCCGTTGTAAGGCGTAGTTTTAAAAGAGAAATGATTTCTGTACAATTAAATGTGGACAACTTTCGCGGTAACTTTTTAAGTGGTTAATGCAATTTAGATGGTTTATGGATATATCAGGTGTTGAAACTCATGTACCACCAATACCAATGTAACTTCATTAACCACAAATTTGAACGTGGTTAACCATTATTTTCAGATGTTCAACTCCATGTAGATTTGAACACCTGGAAATTATGGTCAATGACATCCAAATTTATGGTTTATGATGAATTTTGAAACATGGTATGTCCATAAACCATCTGTTTAACGTAATTAATACCTATTCGAAGTGCATTAACCACTTATAGAAGTTGTAGCCAAAGCTGTCTAAATTTCGTTGTTCAAAATGTGCAACTGTTTTAAAATGCTCAGTTAGAGAATTTTAATTGTTAAGAATAGCTTGCTATGAATAGTTAGATTTTGAAGTATAATGACAATAGAACTGCTTGTGTATTAGCTTATCTGTTAATATATTTTGTTGTCTGGTTATGTTACTTGAGAAATTTGCTCGTGTTTATCCTTAGGATTAAGTTAGGAAATGGCTTAAACTGTAGTTTCAAACTTGTGTTTTGAAATCACCTAAATTGAACAATCAAAGTTTCAGCCTGAAATGGGAGGATGGGAAGTAAGGTGATTTTAGTTTTATTTGCTAATGTATTGAGTTGCAGCCGTGTGACGTTCATCTGTGGCCGTGCCGGTGTTTGTGCTCTTGGTGCTGTTATAGCTAAGCATGCTGGTGATGAGAGATTACTTGACTATTACCTTAGACAATTCAAAGAGGTATTTTGTTGTATTTATTTATGAATCACGATCTTATGATAGTCCAATTTACACATGAATTTCGGGTGTTAAACTTCTTTTTCGTATTGGTGCTTTTATTTTTGGAACAGATTGAGCTACCCACTGATTTGCCCTATGAGTTATTGTATGGCAGAACAGGTTACTTGTGGGCTTGTTCATTCTTAAACAAGCATATCGGTAAAGACACAATACCCACTACCTACACGGTAAGGCATATGTTCTTCATCATGGCGTTCTTCTTTGCAATTTAACTTGTCAAAATCATGTCGACTTGCCATGAAAATTGGCTTTTAATTTAATTTTTGACAGAGACCAATTGTGGATGAAGTTATAACAGCTGGTAGAAAATTAGCTCACATGGGAAGATGTCCTTTGATGTATGAATGGCATGGGAAGAAATACTGGGGTGCTGCTCATGGACTTGCAGGAATTATGAACGTTTTGATGGACATGAAACTTAAGCCAGATGAGGTGGAAGATGTCAAGGGTACGCTACGCTACATGATTAAAAATCGTTTCTCGAGTGGCAATTATCCCTCCAGTGAAGGAAGTGAATCTGACCATCTTGTGCATTGGTGTCACGGTGCTCCTGGAGTAACTCTTACGCTTGTAAAAGCAGCCGAGGTAATATTTCCCTTTATTTTTTTTTATAATGAGGCAATCTTCTCATTCGTTACCATATTCAAATACAAGCTTCACGCCGAGTCAATGTGATGTAAAGGAACCAATAAATTTGCACAAACCTGATTTCAATGGATTTTGCAGTGTTCATTGTATGTGGTGTACTTAAACAGTTAAACTGAAAGTGATCTTGACAATTCAATGAAAATTAAGATCACATGACTAATTTCTTTACCATAATTAGGTTTTTGGGGATAGGGAATTTTCGCAAGCAGCGGAAGATGCAGGAGAAGTAGTGTGGAAGAGGGGTCTTCTTAAGCGAGTTGGAATTTGCCACGGCATCAGTGGGAACACCTATGTCTTTCTTTCTCTGTACAGATTGACAGGAAACAAGGAGTACTTGTACAGGGCTAAAGGATTTTCTTGCTTTCTACTTGATAGAGCGCAAGAATTGATCGGCCAAGGAAAGATGCATGGAGGAGATCGCCCTTATTCCTTATTCGAAGGTCTTGGAGGAATGGCATATACCTTTCTAGACATGATTGATCCACAAGTGGCTAGGTTCCCTGGCTATGAACTTTGA
Protein sequence

>MS.gene059857.t1

MSDRFFPNNLPPFMPETTLEEAEAEATSSQTPTPHSLTSLLSLSYQTLTSKLQVTALHFKQLVVSETWGSSGNRVKDYTLYTGVLGTAYLVFKVYQVTKNVDDLSLCLKIVKACDSASANSSRVTFICGRAGVCALGAVIAKHAGDERLLDYYLRQFKEIELPTDLPYELLYGRTGYLWACSFLNKHIGKDTIPTTYTRPIVDEVITAGRKLAHMGRCPLMYEWHGKKYWGAAHGLAGIMNVLMDMKLKPDEVEDVKGTLRYMIKNRFSSGNYPSSEGSESDHLVHWCHGAPGVTLTLVKAAEVFGDREFSQAAEDAGEVVWKRGLLKRVGICHGISGNTYVFLSLYRLTGNKEYLYRAKGFSCFLLDRAQELIGQGKMHGGDRPYSLFEGLGGMAYTFLDMIDPQVARFPGYEL