AlfalfaGEDB | MS.gene060299

M. sativa cultivar XinJiangDaYe / MS.gene060299

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060299.t1	XP_013470020.1	97.1	103	3	0	1	103	1	103	1.70E-44	188.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060299.t1	A0A072VRJ2	97.1	103	3	0	1	103	1	103	1.2e-44	188.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene060299	MS.gene067331	0.818373	2.03E-52	-1.69E-46
MS.gene060299	MS.gene08521	0.824815	6.58E-54	-1.69E-46
MS.gene060299	MS.gene08869	0.811466	6.90E-51	-1.69E-46
MS.gene060299	MS.gene26792	0.80157	8.46E-49	-1.69E-46
MS.gene060299	MS.gene31056	0.818309	2.10E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060299.t1	MTR_1g107085	97.087	103	3	0	1	103	1	103	4.76e-65	191
MS.gene060299.t1	MTR_1g107085	93.814	97	6	0	1	97	1	97	1.05e-57	173
MS.gene060299.t1	MTR_1g107085	96.809	94	3	0	1	94	1	94	1.71e-57	172
MS.gene060299.t1	MTR_1g107085	95.789	95	4	0	1	95	1	95	2.51e-47	146
MS.gene060299.t1	MTR_5g016330	49.485	97	36	4	1	93	1	88	1.02e-12	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060299.t1	AT2G15000	56.190	105	40	4	1	103	1	101	5.39e-25	90.5
MS.gene060299.t1	AT2G15000	56.190	105	40	4	1	103	1	101	5.39e-25	90.5
MS.gene060299.t1	AT2G15000	56.190	105	40	4	1	103	1	101	5.39e-25	90.5
MS.gene060299.t1	AT2G15000	56.190	105	40	4	1	103	1	101	5.39e-25	90.5
MS.gene060299.t1	AT2G15000	54.639	97	38	4	1	95	1	93	7.90e-19	74.7
MS.gene060299.t1	AT4G34265	47.368	114	33	5	1	103	1	98	1.59e-18	73.9
MS.gene060299.t1	AT4G34265	47.368	114	33	5	1	103	1	98	1.59e-18	73.9
MS.gene060299.t1	AT4G34265	47.368	114	33	5	1	103	1	98	1.59e-18	73.9
MS.gene060299.t1	AT2G15000	54.167	96	38	4	1	94	1	92	3.86e-18	73.2
MS.gene060299.t1	AT2G15000	54.167	96	38	4	1	94	1	92	3.86e-18	73.2
MS.gene060299.t1	AT4G34265	45.283	106	31	5	1	95	1	90	1.54e-13	61.2
MS.gene060299.t1	AT4G34265	44.340	106	32	5	1	95	1	90	6.59e-13	59.7
MS.gene060299.t1	AT4G34265	44.340	106	32	5	1	95	1	90	6.59e-13	59.7

Find 43 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCATGCCTGCTACCTCTTCC+AGG	0.234541	1.2:-79729726	MS.gene060299:intron
GTGCAGGAATTTGGGGCAAT+TGG	0.247985	1.2:-79729610	MS.gene060299:intron
TGCAGGAATTTGGGGCAATT+GGG	0.381549	1.2:-79729609	MS.gene060299:intron
CGGAGGCGAGGAAGGGAAGA+AGG	0.397485	1.2:+79729793	None:intergenic
ATAACAATGGCGTTGCGATC+TGG	0.400559	1.2:-79729871	None:intergenic
TGGCGAGTGGCGGAGGACGG+AGG	0.410736	1.2:+79729776	None:intergenic
CGAGGGGTGGCGAGTGGCGG+AGG	0.419804	1.2:+79729769	None:intergenic
GCGGAGGACGGAGGCGAGGA+AGG	0.437115	1.2:+79729785	None:intergenic
AGAAGGAGCCGCCGGACGAA+GGG	0.456281	1.2:+79729810	None:intergenic
AAGAGGCGGGGCTGAGTGCG+AGG	0.481512	1.2:+79729751	None:intergenic
GGCCAGGTCTTCCCTTCGTC+CGG	0.483656	1.2:-79729821	MS.gene060299:CDS
AGCAGGCATGGAAAAGAGGC+GGG	0.485396	1.2:+79729738	None:intergenic
AGACCTGGCCGTGGAGATGA+AGG	0.492112	1.2:+79729834	None:intergenic
GCTCCTTCATCTCCACGGCC+AGG	0.498839	1.2:-79729837	MS.gene060299:CDS
CGGAGGACGGAGGCGAGGAA+GGG	0.500771	1.2:+79729786	None:intergenic
GTGCGAGGGGTGGCGAGTGG+CGG	0.502077	1.2:+79729766	None:intergenic
CCTGGAAGAGGTAGCAGGCA+TGG	0.513058	1.2:+79729726	None:intergenic
CGCCGGACGAAGGGAAGACC+TGG	0.513153	1.2:+79729819	None:intergenic
GGGTGGCGAGTGGCGGAGGA+CGG	0.517554	1.2:+79729773	None:intergenic
TTAGGGTTTGGACCTGGAAG+AGG	0.524458	1.2:+79729714	None:intergenic
TTGGACCTGGAAGAGGTAGC+AGG	0.529380	1.2:+79729721	None:intergenic
AGGTAGCAGGCATGGAAAAG+AGG	0.531493	1.2:+79729734	None:intergenic
AGTGGCGGAGGACGGAGGCG+AGG	0.533816	1.2:+79729781	None:intergenic
ACGAAGGGAAGACCTGGCCG+TGG	0.538799	1.2:+79729825	None:intergenic
AGAGGCGGGGCTGAGTGCGA+GGG	0.539518	1.2:+79729752	None:intergenic
AAGAAGGAGCCGCCGGACGA+AGG	0.539975	1.2:+79729809	None:intergenic
ATCTCGCTCCTTCATCTCCA+CGG	0.540316	1.2:-79729842	MS.gene060299:CDS
AAGCTCGTGCGCTGACGGCA+AGG	0.543327	1.2:+79729526	None:intergenic
GAGGCGGGGCTGAGTGCGAG+GGG	0.547076	1.2:+79729753	None:intergenic
GGAAGGGAAGAAGGAGCCGC+CGG	0.548993	1.2:+79729802	None:intergenic
GCGGGGCTGAGTGCGAGGGG+TGG	0.550387	1.2:+79729756	None:intergenic
TGAGTGCGAGGGGTGGCGAG+TGG	0.550653	1.2:+79729763	None:intergenic
GCTACAAGCTCGTGCGCTGA+CGG	0.567501	1.2:+79729521	None:intergenic
AGAACGACGGAAGGTACCAT+TGG	0.577356	1.2:+79729491	None:intergenic
TAGCAGGCATGGAAAAGAGG+CGG	0.583179	1.2:+79729737	None:intergenic
TTTGTTGGTGCAGGAATTTG+GGG	0.587970	1.2:-79729617	MS.gene060299:intron
GCTTGTAGCGAGCTGTCCAA+TGG	0.595505	1.2:-79729507	MS.gene060299:CDS
ACAGCCATCGTGCTATGCAG+TGG	0.621490	1.2:+79729567	None:intergenic
ATCTTGACAAGAACGACGGA+AGG	0.626544	1.2:+79729482	None:intergenic
CAGGTCTTCCCTTCGTCCGG+CGG	0.627826	1.2:-79729818	MS.gene060299:CDS
GCAGGCATGGAAAAGAGGCG+GGG	0.645770	1.2:+79729739	None:intergenic
TTTGCCACTGCATAGCACGA+TGG	0.666045	1.2:-79729571	MS.gene060299:CDS
AGCGATCTTGACAAGAACGA+CGG	0.707528	1.2:+79729478	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TCTGAATGTGTTTTTTTTGT+TGG	-	chr1.2:79729709-79729728	MS.gene060299:intron	25.0%
	ATTGTCGTTGAGCTTGAATT+GGG	+	chr1.2:79729655-79729674	None:intergenic	35.0%
	TGAGCTTGAATTGGGAATTA+GGG	+	chr1.2:79729647-79729666	None:intergenic	35.0%
	TTGAATTGGGAATTAGGGTT+TGG	+	chr1.2:79729642-79729661	None:intergenic	35.0%
	TTGAGCTTGAATTGGGAATT+AGG	+	chr1.2:79729648-79729667	None:intergenic	35.0%
!	TTTTGTTGGTGCAGGAATTT+GGG	-	chr1.2:79729723-79729742	MS.gene060299:intron	35.0%
!	TTTTTGTTGGTGCAGGAATT+TGG	-	chr1.2:79729722-79729741	MS.gene060299:intron	35.0%
!!!	TGTGTTTTTTTTGTTGGTGC+AGG	-	chr1.2:79729715-79729734	MS.gene060299:intron	35.0%
	CATTGTCGTTGAGCTTGAAT+TGG	+	chr1.2:79729656-79729675	None:intergenic	40.0%
	TTTGTTGGTGCAGGAATTTG+GGG	-	chr1.2:79729724-79729743	MS.gene060299:intron	40.0%
	AGCGATCTTGACAAGAACGA+CGG	+	chr1.2:79729866-79729885	None:intergenic	45.0%
	ATCTTGACAAGAACGACGGA+AGG	+	chr1.2:79729862-79729881	None:intergenic	45.0%
!	TGCAGGAATTTGGGGCAATT+GGG	-	chr1.2:79729732-79729751	MS.gene060299:CDS	45.0%
	AGGTAGCAGGCATGGAAAAG+AGG	+	chr1.2:79729610-79729629	None:intergenic	50.0%
	ATCTCGCTCCTTCATCTCCA+CGG	-	chr1.2:79729499-79729518	MS.gene060299:CDS	50.0%
	TAGCAGGCATGGAAAAGAGG+CGG	+	chr1.2:79729607-79729626	None:intergenic	50.0%
	TGGGAATTAGGGTTTGGACC+TGG	+	chr1.2:79729636-79729655	None:intergenic	50.0%
	TTAGGGTTTGGACCTGGAAG+AGG	+	chr1.2:79729630-79729649	None:intergenic	50.0%
	TTTGCCACTGCATAGCACGA+TGG	-	chr1.2:79729770-79729789	MS.gene060299:CDS	50.0%
!	GTGCAGGAATTTGGGGCAAT+TGG	-	chr1.2:79729731-79729750	MS.gene060299:CDS	50.0%
!!	AGAACGACGGAAGGTACCAT+TGG	+	chr1.2:79729853-79729872	None:intergenic	50.0%
	ACAGCCATCGTGCTATGCAG+TGG	+	chr1.2:79729777-79729796	None:intergenic	55.0%
	AGCAGGCATGGAAAAGAGGC+GGG	+	chr1.2:79729606-79729625	None:intergenic	55.0%
	GCTTGTAGCGAGCTGTCCAA+TGG	-	chr1.2:79729834-79729853	MS.gene060299:CDS	55.0%
	TTGGACCTGGAAGAGGTAGC+AGG	+	chr1.2:79729623-79729642	None:intergenic	55.0%
	AGACCTGGCCGTGGAGATGA+AGG	+	chr1.2:79729510-79729529	None:intergenic	60.0%
	CCATGCCTGCTACCTCTTCC+AGG	-	chr1.2:79729615-79729634	MS.gene060299:intron	60.0%
	CCTGGAAGAGGTAGCAGGCA+TGG	+	chr1.2:79729618-79729637	None:intergenic	60.0%
	GCAGGCATGGAAAAGAGGCG+GGG	+	chr1.2:79729605-79729624	None:intergenic	60.0%
	GCTACAAGCTCGTGCGCTGA+CGG	+	chr1.2:79729823-79729842	None:intergenic	60.0%
	AAGAAGGAGCCGCCGGACGA+AGG	+	chr1.2:79729535-79729554	None:intergenic	65.0%
	ACGAAGGGAAGACCTGGCCG+TGG	+	chr1.2:79729519-79729538	None:intergenic	65.0%
	AGAAGGAGCCGCCGGACGAA+GGG	+	chr1.2:79729534-79729553	None:intergenic	65.0%
	CAGGTCTTCCCTTCGTCCGG+CGG	-	chr1.2:79729523-79729542	MS.gene060299:CDS	65.0%
	CGGAGGCGAGGAAGGGAAGA+AGG	+	chr1.2:79729551-79729570	None:intergenic	65.0%
	GCTCCTTCATCTCCACGGCC+AGG	-	chr1.2:79729504-79729523	MS.gene060299:CDS	65.0%
	GGAAGGGAAGAAGGAGCCGC+CGG	+	chr1.2:79729542-79729561	None:intergenic	65.0%
	GGCCAGGTCTTCCCTTCGTC+CGG	-	chr1.2:79729520-79729539	MS.gene060299:CDS	65.0%
!	AAGCTCGTGCGCTGACGGCA+AGG	+	chr1.2:79729818-79729837	None:intergenic	65.0%
	AAGAGGCGGGGCTGAGTGCG+AGG	+	chr1.2:79729593-79729612	None:intergenic	70.0%
	AGAGGCGGGGCTGAGTGCGA+GGG	+	chr1.2:79729592-79729611	None:intergenic	70.0%
	CGCCGGACGAAGGGAAGACC+TGG	+	chr1.2:79729525-79729544	None:intergenic	70.0%
	CGGAGGACGGAGGCGAGGAA+GGG	+	chr1.2:79729558-79729577	None:intergenic	70.0%
	TGAGTGCGAGGGGTGGCGAG+TGG	+	chr1.2:79729581-79729600	None:intergenic	70.0%
	GAGGCGGGGCTGAGTGCGAG+GGG	+	chr1.2:79729591-79729610	None:intergenic	75.0%
	GCGGAGGACGGAGGCGAGGA+AGG	+	chr1.2:79729559-79729578	None:intergenic	75.0%
	GTGCGAGGGGTGGCGAGTGG+CGG	+	chr1.2:79729578-79729597	None:intergenic	75.0%
!	AGTGGCGGAGGACGGAGGCG+AGG	+	chr1.2:79729563-79729582	None:intergenic	75.0%
!	GGGTGGCGAGTGGCGGAGGA+CGG	+	chr1.2:79729571-79729590	None:intergenic	75.0%
!	TGGCGAGTGGCGGAGGACGG+AGG	+	chr1.2:79729568-79729587	None:intergenic	75.0%
!	CGAGGGGTGGCGAGTGGCGG+AGG	+	chr1.2:79729575-79729594	None:intergenic	80.0%
!	GCGGGGCTGAGTGCGAGGGG+TGG	+	chr1.2:79729588-79729607	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	79729476	79729887	79729476	ID=MS.gene060299
chr1.2	mRNA	79729476	79729887	79729476	ID=MS.gene060299.t1;Parent=MS.gene060299
chr1.2	exon	79729727	79729887	79729727	ID=MS.gene060299.t1.exon1;Parent=MS.gene060299.t1
chr1.2	CDS	79729727	79729887	79729727	ID=cds.MS.gene060299.t1;Parent=MS.gene060299.t1
chr1.2	exon	79729476	79729626	79729476	ID=MS.gene060299.t1.exon2;Parent=MS.gene060299.t1
chr1.2	CDS	79729476	79729626	79729476	ID=cds.MS.gene060299.t1;Parent=MS.gene060299.t1

Gene Sequence

>MS.gene060299

ATGGCGTTGCGATCTGGATCTCTATCTCGCTCCTTCATCTCCACGGCCAGGTCTTCCCTTCGTCCGGCGGCTCCTTCTTCCCTTCCTCGCCTCCGTCCTCCGCCACTCGCCACCCCTCGCACTCAGCCCCGCCTCTTTTCCATGCCTGCTACCTCTTCCAGGTCCAAACCCTAATTCCCAATTCAAGCTCAACGACAATGAAATTGATTATTAATTTGATTGATATTGTTGATTCTGAATGTGTTTTTTTTGTTGGTGCAGGAATTTGGGGCAATTGGGTTGCGTACAATCACTTTTGCCACTGCATAGCACGATGGCTGTAGCGTGTCTCACGTCTCACCTTGCCGTCAGCGCACGAGCTTGTAGCGAGCTGTCCAATGGTACCTTCCGTCGTTCTTGTCAAGATCGCTAA

Protein sequence

>MS.gene060299.t1

MALRSGSLSRSFISTARSSLRPAAPSSLPRLRPPPLATPRTQPRLFSMPATSSRNLGQLGCVQSLLPLHSTMAVACLTSHLAVSARACSELSNGTFRRSCQDR