AlfalfaGEDB | MS.gene08521

M. sativa cultivar XinJiangDaYe / MS.gene08521

KEGG: K02942
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006414 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0034645 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044249 GO:0044260 GO:0071704 GO:0110165 GO:1901576
PFAM: PF00428.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08521.t1	XP_013444548.1	100	60	0	0	1	60	1	60	7.90E-24	119.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08521.t1	Q8LCW9	61.7	60	23	0	2	61	3	62	6.1e-17	88.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08521.t1	I3S0D9	100.0	60	0	0	1	60	1	60	5.7e-24	119.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049361	MS.gene08521	0.827667	1.38E-54	-1.69E-46
MS.gene049387	MS.gene08521	0.849535	3.04E-60	-1.69E-46
MS.gene050134	MS.gene08521	0.905595	3.64E-80	-1.69E-46
MS.gene050252	MS.gene08521	0.846495	2.10E-59	-1.69E-46
MS.gene050638	MS.gene08521	-0.813076	3.07E-51	-1.69E-46
MS.gene050791	MS.gene08521	0.897584	1.22E-76	-1.69E-46
MS.gene050912	MS.gene08521	0.834636	2.67E-56	-1.69E-46
MS.gene051904	MS.gene08521	0.801959	7.04E-49	-1.69E-46
MS.gene051984	MS.gene08521	0.8884	6.11E-73	-1.69E-46
MS.gene052412	MS.gene08521	0.80745	5.02E-50	-1.69E-46
MS.gene052664	MS.gene08521	0.822807	1.94E-53	-1.69E-46
MS.gene052666	MS.gene08521	0.856589	2.90E-62	-1.69E-46
MS.gene053224	MS.gene08521	0.887049	2.01E-72	-1.69E-46
MS.gene054624	MS.gene08521	0.833704	4.58E-56	-1.69E-46
MS.gene055232	MS.gene08521	0.813662	2.28E-51	-1.69E-46
MS.gene057272	MS.gene08521	0.910401	1.95E-82	-1.69E-46
MS.gene057321	MS.gene08521	0.851099	1.11E-60	-1.69E-46
MS.gene057641	MS.gene08521	0.828846	7.16E-55	-1.69E-46
MS.gene057643	MS.gene08521	0.88462	1.64E-71	-1.69E-46
MS.gene059340	MS.gene08521	0.858564	7.55E-63	-1.69E-46
MS.gene060299	MS.gene08521	0.824815	6.58E-54	-1.69E-46
MS.gene060483	MS.gene08521	0.951935	7.11E-110	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene82009	MS.gene08521	PPI
MS.gene073078	MS.gene08521	PPI
MS.gene069339	MS.gene08521	PPI
MS.gene08521	MS.gene032942	PPI
MS.gene009874	MS.gene08521	PPI
MS.gene08521	MS.gene35379	PPI
MS.gene08521	MS.gene06617	PPI
MS.gene08521	MS.gene88792	PPI
MS.gene44900	MS.gene08521	PPI
MS.gene08521	MS.gene047578	PPI
MS.gene08521	MS.gene002442	PPI
MS.gene08521	MS.gene045633	PPI
MS.gene08521	MS.gene057237	PPI
MS.gene08521	MS.gene01091	PPI
MS.gene08521	MS.gene91826	PPI
MS.gene08521	MS.gene78764	PPI
MS.gene08521	MS.gene97876	PPI
MS.gene08521	MS.gene053952	PPI
MS.gene042719	MS.gene08521	PPI
MS.gene06833	MS.gene08521	PPI
MS.gene013506	MS.gene08521	PPI
MS.gene76615	MS.gene08521	PPI
MS.gene08521	MS.gene060483	PPI
MS.gene08521	MS.gene067331	PPI
MS.gene08521	MS.gene76615	PPI
MS.gene43071	MS.gene08521	PPI
MS.gene08521	MS.gene01952	PPI
MS.gene08521	MS.gene01425	PPI
MS.gene08521	MS.gene82009	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08521.t1	MTR_8g024350	98.148	108	2	0	1	108	1	108	1.26e-72	211
MS.gene08521.t1	MTR_3g092600	60.000	110	42	1	1	108	1	110	4.73e-29	101
MS.gene08521.t1	MTR_2g066750	60.909	110	41	1	1	108	1	110	4.73e-29	101
MS.gene08521.t1	MTR_7g118060	66.102	59	20	0	1	59	1	59	1.53e-23	87.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08521.t1	AT5G24510	57.391	115	38	4	1	108	1	111	1.06e-37	123
MS.gene08521.t1	AT1G01100	51.402	107	39	1	2	108	3	96	7.04e-32	108
MS.gene08521.t1	AT4G00810	53.153	111	48	1	2	108	3	113	2.94e-31	107
MS.gene08521.t1	AT4G00810	53.153	111	48	1	2	108	3	113	2.94e-31	107
MS.gene08521.t1	AT1G01100	53.636	110	48	1	2	108	3	112	6.42e-31	106
MS.gene08521.t1	AT1G01100	53.636	110	48	1	2	108	3	112	6.42e-31	106
MS.gene08521.t1	AT1G01100	53.636	110	48	1	2	108	3	112	6.42e-31	106
MS.gene08521.t1	AT5G47700	53.153	111	48	1	2	108	3	113	9.37e-31	105
MS.gene08521.t1	AT5G47700	53.153	111	48	1	2	108	3	113	9.37e-31	105

Find 28 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGGAGGCAGCAGCAGCAAC+TGG	0.216487	8.3:-68089016	None:intergenic
ACGTTAATGTTGAAGCTTAC+TGG	0.234944	8.3:+68088900	MS.gene08521:CDS
TGGTGCTCCGGTTTCTGTTT+CGG	0.321080	8.3:+68088991	MS.gene08521:CDS
GTCGTCGTCACTCTCATCCT+TGG	0.357062	8.3:-68089166	None:intergenic
ATCATCAAGGTTCTTCTTCT+CGG	0.364681	8.3:-68088945	None:intergenic
ACTGGGGCCGAAACAGAAAC+CGG	0.462684	8.3:-68088998	None:intergenic
TCGTCGTCACTCTCATCCTT+GGG	0.464875	8.3:-68089165	None:intergenic
TGCCGCTGGAGGTGGTGCTC+CGG	0.491299	8.3:+68088979	MS.gene08521:CDS
ATATTGAATGCCGCTGGAGG+TGG	0.496313	8.3:+68088971	MS.gene08521:CDS
GCAAGTTTAGCGAATAAACT+TGG	0.520693	8.3:-68088923	None:intergenic
TGCTCCTATCGAGGAGAAGA+AGG	0.520970	8.3:+68089048	MS.gene08521:CDS
TGGAGGCAGCAGCAGCAACT+GGG	0.544694	8.3:-68089015	None:intergenic
AGGAGCAGCAACTGCAGTGG+AGG	0.545209	8.3:-68089032	None:intergenic
GGATGAGAGTGACGACGACA+TGG	0.547177	8.3:+68089170	MS.gene08521:CDS
GTTTGCAGCTTTCAACAAAG+TGG	0.560297	8.3:-68088879	None:intergenic
GATTTGATATTGAATGCCGC+TGG	0.580711	8.3:+68088965	MS.gene08521:CDS
AACCGGAGCACCACCTCCAG+CGG	0.583475	8.3:-68088981	None:intergenic
CGGCGATGTTATCATCATGA+AGG	0.588233	8.3:-68088164	None:intergenic
CATTCAATATCAAATCATCA+AGG	0.592309	8.3:-68088958	None:intergenic
TGCAGTTGCTGCTCCTATCG+AGG	0.607768	8.3:+68089039	MS.gene08521:CDS
GGAGGCAGCAGCAGCAACTG+GGG	0.621755	8.3:-68089014	None:intergenic
CCTACCTTCTTCTCCTCGAT+AGG	0.622145	8.3:-68089052	None:intergenic
GATAGGAGCAGCAACTGCAG+TGG	0.632714	8.3:-68089035	None:intergenic
TGTAGTGTGAAAATGTCGAT+CGG	0.636502	8.3:+68088111	None:intergenic
ATTATCAAAGGATACAGTGA+CGG	0.653291	8.3:-68088184	None:intergenic
GATGAGAGTGACGACGACAT+GGG	0.653294	8.3:+68089171	MS.gene08521:CDS
GTACTTACAGGAAGAACCCA+AGG	0.654187	8.3:+68089149	MS.gene08521:intron
TTGATATTGAATGCCGCTGG+AGG	0.659375	8.3:+68088968	MS.gene08521:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAAAAATTAGGTTTTTTAA+AGG	+	chr8.3:68088573-68088592	MS.gene08521:intron	10.0%
!!!	AAAATCAATTTTTATATAGT+GGG	+	chr8.3:68088724-68088743	MS.gene08521:intron	10.0%
!!!	TAAAAAACCTAATTTTTTTA+AGG	-	chr8.3:68088572-68088591	None:intergenic	10.0%
!!!	TAAAATCAATTTTTATATAG+TGG	+	chr8.3:68088723-68088742	MS.gene08521:intron	10.0%
!!	AAAAATTCAGTAATAATTTG+AGG	-	chr8.3:68088440-68088459	None:intergenic	15.0%
!!	AATTAGTCCTTAAAAAAATT+AGG	+	chr8.3:68088562-68088581	MS.gene08521:intron	15.0%
!!	ATTACATTATCAATTATCAA+AGG	-	chr8.3:68088199-68088218	None:intergenic	15.0%
!!!	AAAAATTGATTTTACAACTT+TGG	-	chr8.3:68088717-68088736	None:intergenic	15.0%
!!!	ATTATTACTGAATTTTTTAC+TGG	+	chr8.3:68088443-68088462	MS.gene08521:intron	15.0%
!!!	TTGAAAAAAAGAGATTTTTT+TGG	+	chr8.3:68088291-68088310	MS.gene08521:intron	15.0%
!!	AAATTCAGAATATAATTCCT+AGG	+	chr8.3:68088501-68088520	MS.gene08521:intron	20.0%
!!!	TCTTATCTAATTGGTATTTT+TGG	+	chr8.3:68089124-68089143	MS.gene08521:intron	20.0%
!	CATTCAATATCAAATCATCA+AGG	-	chr8.3:68088961-68088980	None:intergenic	25.0%
!	TAAGTGATAGTTAAAGTTCT+TGG	+	chr8.3:68088611-68088630	MS.gene08521:intron	25.0%
!	TAATGAATTTAGAAGATGCT+AGG	+	chr8.3:68088259-68088278	MS.gene08521:intron	25.0%
!!	ATTAACAATTTTCTACCGTT+AGG	+	chr8.3:68088689-68088708	MS.gene08521:intron	25.0%
!!	TTAACAATTTTCTACCGTTA+GGG	+	chr8.3:68088690-68088709	MS.gene08521:intron	25.0%
!!!	AGATTTTTTTGGATGATACA+AGG	+	chr8.3:68088302-68088321	MS.gene08521:intron	25.0%
!!!	ATTTTTATATAGTGGGAATC+TGG	+	chr8.3:68088731-68088750	MS.gene08521:intron	25.0%
!!!	ATTTTTTTGGATGATACAAG+GGG	+	chr8.3:68088304-68088323	MS.gene08521:intron	25.0%
!!!	GAATTTTGTTTTGGTGAATA+AGG	+	chr8.3:68088759-68088778	MS.gene08521:intron	25.0%
!!!	GATTTTTTTGGATGATACAA+GGG	+	chr8.3:68088303-68088322	MS.gene08521:intron	25.0%
!!!	TTTTTATATAGTGGGAATCT+GGG	+	chr8.3:68088732-68088751	MS.gene08521:intron	25.0%
	AATGACAATTAACAACTCCT+AGG	-	chr8.3:68088521-68088540	None:intergenic	30.0%
	ATTATCAAAGGATACAGTGA+CGG	-	chr8.3:68088187-68088206	None:intergenic	30.0%
	ATTTAGAAGATGCTAGGAAT+TGG	+	chr8.3:68088265-68088284	MS.gene08521:intron	30.0%
	TAAAGTTCTTGGATAGAGTT+AGG	+	chr8.3:68088622-68088641	MS.gene08521:intron	30.0%
	TAGATAAGATGACCAACAAA+CGG	-	chr8.3:68089113-68089132	None:intergenic	30.0%
	TCCTTAAGTCATTGTTGATA+GGG	+	chr8.3:68088845-68088864	MS.gene08521:intron	30.0%
	TGTTGGTCATCTTATCTAAT+TGG	+	chr8.3:68089115-68089134	MS.gene08521:intron	30.0%
	TTTAGAAGATGCTAGGAATT+GGG	+	chr8.3:68088266-68088285	MS.gene08521:intron	30.0%
!!!	AGATAGCGTGTTTTAAAGAT+TGG	+	chr8.3:68088818-68088837	MS.gene08521:intron	30.0%
!!!	CTGGGTTTTGAATTTTGTTT+TGG	+	chr8.3:68088750-68088769	MS.gene08521:intron	30.0%
!!!	GTATTTTTGGTTGTACTTAC+AGG	+	chr8.3:68089137-68089156	MS.gene08521:intron	30.0%
	ACCCTATCAACAATGACTTA+AGG	-	chr8.3:68088849-68088868	None:intergenic	35.0%
	ACGTTAATGTTGAAGCTTAC+TGG	+	chr8.3:68088900-68088919	MS.gene08521:CDS	35.0%
	ATCATCAAGGTTCTTCTTCT+CGG	-	chr8.3:68088948-68088967	None:intergenic	35.0%
	CTCCTTAAGTCATTGTTGAT+AGG	+	chr8.3:68088844-68088863	MS.gene08521:intron	35.0%
	GCAAGTTTAGCGAATAAACT+TGG	-	chr8.3:68088926-68088945	None:intergenic	35.0%
	TTTACAACTTTGGTCCCTAA+CGG	-	chr8.3:68088707-68088726	None:intergenic	35.0%
	TTTGGTGAATAAGGAAAGTG+TGG	+	chr8.3:68088768-68088787	MS.gene08521:intron	35.0%
!	AATAAGGAAAGTGTGGTGTA+AGG	+	chr8.3:68088775-68088794	MS.gene08521:intron	35.0%
!	ATAAGGAAAGTGTGGTGTAA+GGG	+	chr8.3:68088776-68088795	MS.gene08521:intron	35.0%
	GAACTTTGTCTTCCGTTTGT+TGG	+	chr8.3:68089098-68089117	MS.gene08521:intron	40.0%
	GATGATACAAGGGGCTATAT+AGG	+	chr8.3:68088313-68088332	MS.gene08521:intron	40.0%
	GATTTGATATTGAATGCCGC+TGG	+	chr8.3:68088965-68088984	MS.gene08521:CDS	40.0%
	GTTTGCAGCTTTCAACAAAG+TGG	-	chr8.3:68088882-68088901	None:intergenic	40.0%
!	AGGAGAAGAAGGTAGGTTTT+TGG	+	chr8.3:68089059-68089078	MS.gene08521:intron	40.0%
!!!	TCGATCAGAAGTGTCGTTTT+AGG	-	chr8.3:68088347-68088366	None:intergenic	40.0%
	CGGCGATGTTATCATCATGA+AGG	-	chr8.3:68088167-68088186	None:intergenic	45.0%
	CTTCTGATCGAAGTTGTGTC+TGG	+	chr8.3:68088356-68088375	MS.gene08521:intron	45.0%
	GTACTTACAGGAAGAACCCA+AGG	+	chr8.3:68089149-68089168	MS.gene08521:intron	45.0%
	TTGATATTGAATGCCGCTGG+AGG	+	chr8.3:68088968-68088987	MS.gene08521:CDS	45.0%
!!!	TTTTTTTATTTTCTTATTTT+AGG	+	chr8.3:68088661-68088680	MS.gene08521:intron	5.0%
	ATATTGAATGCCGCTGGAGG+TGG	+	chr8.3:68088971-68088990	MS.gene08521:CDS	50.0%
	CCTACCTTCTTCTCCTCGAT+AGG	-	chr8.3:68089055-68089074	None:intergenic	50.0%
	CCTATCGAGGAGAAGAAGGT+AGG	+	chr8.3:68089052-68089071	MS.gene08521:intron	50.0%
	GATGAGAGTGACGACGACAT+GGG	+	chr8.3:68089171-68089190	MS.gene08521:CDS	50.0%
	TCGTCGTCACTCTCATCCTT+GGG	-	chr8.3:68089168-68089187	None:intergenic	50.0%
	TGCTCCTATCGAGGAGAAGA+AGG	+	chr8.3:68089048-68089067	MS.gene08521:CDS	50.0%
	TGGTGCTCCGGTTTCTGTTT+CGG	+	chr8.3:68088991-68089010	MS.gene08521:CDS	50.0%
	TGGTGTGTGTCAGTGCTTCA+TGG	+	chr8.3:68088463-68088482	MS.gene08521:intron	50.0%
	ACTGGGGCCGAAACAGAAAC+CGG	-	chr8.3:68089001-68089020	None:intergenic	55.0%
	GATAGGAGCAGCAACTGCAG+TGG	-	chr8.3:68089038-68089057	None:intergenic	55.0%
	GGATGAGAGTGACGACGACA+TGG	+	chr8.3:68089170-68089189	MS.gene08521:CDS	55.0%
	GTCGTCGTCACTCTCATCCT+TGG	-	chr8.3:68089169-68089188	None:intergenic	55.0%
	TGCAGTTGCTGCTCCTATCG+AGG	+	chr8.3:68089039-68089058	MS.gene08521:CDS	55.0%
	AGGAGCAGCAACTGCAGTGG+AGG	-	chr8.3:68089035-68089054	None:intergenic	60.0%
	TGGAGGCAGCAGCAGCAACT+GGG	-	chr8.3:68089018-68089037	None:intergenic	60.0%
	GGAGGCAGCAGCAGCAACTG+GGG	-	chr8.3:68089017-68089036	None:intergenic	65.0%
	GTGGAGGCAGCAGCAGCAAC+TGG	-	chr8.3:68089019-68089038	None:intergenic	65.0%
!	AACCGGAGCACCACCTCCAG+CGG	-	chr8.3:68088984-68089003	None:intergenic	65.0%
	TGCCGCTGGAGGTGGTGCTC+CGG	+	chr8.3:68088979-68088998	MS.gene08521:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	68088123	68089212	68088123	ID=MS.gene08521
chr8.3	mRNA	68088123	68089212	68088123	ID=MS.gene08521.t1;Parent=MS.gene08521
chr8.3	exon	68088123	68088191	68088123	ID=MS.gene08521.t1.exon1;Parent=MS.gene08521.t1
chr8.3	CDS	68088123	68088191	68088123	ID=cds.MS.gene08521.t1;Parent=MS.gene08521.t1
chr8.3	exon	68088866	68089069	68088866	ID=MS.gene08521.t1.exon2;Parent=MS.gene08521.t1
chr8.3	CDS	68088866	68089069	68088866	ID=cds.MS.gene08521.t1;Parent=MS.gene08521.t1
chr8.3	exon	68089159	68089212	68089159	ID=MS.gene08521.t1.exon3;Parent=MS.gene08521.t1
chr8.3	CDS	68089159	68089212	68089159	ID=cds.MS.gene08521.t1;Parent=MS.gene08521.t1

Gene Sequence

>MS.gene08521

ATGTCGATCGGAGAAGTTGCTTGCAGCTACTCACTTATGATCCTTCATGATGATAACATCGCCGTCACTGTATCCTTTGATAATTGATAATGTAATTAATTTAATTATGATTTTCTTTTGTTAGTATATGTTTTTTTAATGAATTTAGAAGATGCTAGGAATTGGGTTTTGAAAAAAAGAGATTTTTTTGGATGATACAAGGGGCTATATAGGATTATGAACCTAAAACGACACTTCTGATCGAAGTTGTGTCTGGTGTATTTTGCGTGTTTTGTCAGCGCACTCACATTCAATTACATTTGATTAATTCATTTCCTCAAATTATTACTGAATTTTTTACTGGTGTGTGTCAGTGCTTCATGGTCTCTGATGCAAGTGAAATTCAGAATATAATTCCTAGGAGTTGTTAATTGTCATTTAGATTCAATTATTGAAATCAAATTAGTCCTTAAAAAAATTAGGTTTTTTAAAGGTTAAACTAGTTTTGATAAGTGATAGTTAAAGTTCTTGGATAGAGTTAGGATAATGTTTTTGTGATTTTTTTTATTTTCTTATTTTAGGACTAAATTAACAATTTTCTACCGTTAGGGACCAAAGTTGTAAAATCAATTTTTATATAGTGGGAATCTGGGTTTTGAATTTTGTTTTGGTGAATAAGGAAAGTGTGGTGTAAGGGTTTGAAGTTGAATATTTGAAGATAGCGTGTTTTAAAGATTGGTTTCTCCTTAAGTCATTGTTGATAGGGTGAGAAAATCTCCACTTTGTTGAAAGCTGCAAACGTTAATGTTGAAGCTTACTGGCCAAGTTTATTCGCTAAACTTGCCGAGAAGAAGAACCTTGATGATTTGATATTGAATGCCGCTGGAGGTGGTGCTCCGGTTTCTGTTTCGGCCCCAGTTGCTGCTGCTGCCTCCACTGCAGTTGCTGCTCCTATCGAGGAGAAGAAGGTAGGTTTTTGGTGAAAAAGTTGTTGTTGAACTTTGTCTTCCGTTTGTTGGTCATCTTATCTAATTGGTATTTTTGGTTGTACTTACAGGAAGAACCCAAGGATGAGAGTGACGACGACATGGGATTCAGCTTGTTTGATTAG

Protein sequence

>MS.gene08521.t1

MSIGEVACSYSLMILHDDNIAVTGEKISTLLKAANVNVEAYWPSLFAKLAEKKNLDDLILNAAGGGAPVSVSAPVAAAASTAVAAPIEEKKEEPKDESDDDMGFSLFD