Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08869.t1 | XP_013468200.1 | 100 | 150 | 0 | 0 | 1 | 150 | 1 | 150 | 3.70E-75 | 290.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08869.t1 | Q9FF52 | 94.0 | 150 | 9 | 0 | 1 | 150 | 1 | 150 | 6.0e-74 | 278.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08869.t1 | B7FH91 | 100.0 | 150 | 0 | 0 | 1 | 150 | 1 | 150 | 2.7e-75 | 290.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049306 | MS.gene08869 | 0.878998 | 1.78E-69 | -1.69E-46 |
| MS.gene049387 | MS.gene08869 | 0.848428 | 6.17E-60 | -1.69E-46 |
| MS.gene050134 | MS.gene08869 | 0.950487 | 1.49E-108 | -1.69E-46 |
| MS.gene050252 | MS.gene08869 | 0.842375 | 2.69E-58 | -1.69E-46 |
| MS.gene050791 | MS.gene08869 | 0.844986 | 5.38E-59 | -1.69E-46 |
| MS.gene050912 | MS.gene08869 | 0.800152 | 1.65E-48 | -1.69E-46 |
| MS.gene051925 | MS.gene08869 | 0.879962 | 8.11E-70 | -1.69E-46 |
| MS.gene051927 | MS.gene08869 | 0.853511 | 2.28E-61 | -1.69E-46 |
| MS.gene051984 | MS.gene08869 | 0.838127 | 3.46E-57 | -1.69E-46 |
| MS.gene052664 | MS.gene08869 | 0.846359 | 2.28E-59 | -1.69E-46 |
| MS.gene052666 | MS.gene08869 | 0.893015 | 9.31E-75 | -1.69E-46 |
| MS.gene053224 | MS.gene08869 | 0.859665 | 3.53E-63 | -1.69E-46 |
| MS.gene054624 | MS.gene08869 | 0.848991 | 4.31E-60 | -1.69E-46 |
| MS.gene057272 | MS.gene08869 | 0.911659 | 4.73E-83 | -1.69E-46 |
| MS.gene057321 | MS.gene08869 | 0.838579 | 2.64E-57 | -1.69E-46 |
| MS.gene057641 | MS.gene08869 | 0.829122 | 6.14E-55 | -1.69E-46 |
| MS.gene057643 | MS.gene08869 | 0.871826 | 5.08E-67 | -1.69E-46 |
| MS.gene059340 | MS.gene08869 | 0.878004 | 3.99E-69 | -1.69E-46 |
| MS.gene060299 | MS.gene08869 | 0.811466 | 6.90E-51 | -1.69E-46 |
| MS.gene060483 | MS.gene08869 | 0.893461 | 6.15E-75 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08869.t1 | MTR_4g059400 | 98.684 | 152 | 2 | 0 | 1 | 152 | 1 | 152 | 1.58e-104 | 296 |
| MS.gene08869.t1 | MTR_1g064500 | 98.684 | 152 | 2 | 0 | 1 | 152 | 1 | 152 | 1.97e-104 | 296 |
| MS.gene08869.t1 | MTR_2g094300 | 82.895 | 152 | 26 | 0 | 1 | 152 | 1 | 152 | 1.14e-89 | 258 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08869.t1 | AT5G60670 | 92.763 | 152 | 11 | 0 | 1 | 152 | 1 | 152 | 2.31e-99 | 283 |
| MS.gene08869.t1 | AT3G53430 | 90.789 | 152 | 14 | 0 | 1 | 152 | 1 | 152 | 3.79e-98 | 280 |
| MS.gene08869.t1 | AT2G37190 | 92.000 | 150 | 12 | 0 | 1 | 150 | 1 | 150 | 4.28e-98 | 280 |
Find 0 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACTAAGAACATCAAGCATAA+TGG | + | 32374:9886-9905 | MS.gene08869:CDS | 30.0% | |
| CTAAGAACATCAAGCATAAT+GGG | + | 32374:9887-9906 | MS.gene08869:CDS | 30.0% | |
| ! | TGATGTTCTTAGTCTTCTTG+CGG | - | 32374:9879-9898 | MS.gene08869:intergenic | 35.0% |
| ATGTCTTCTCCGATCTTCTT+TGG | - | 32374:9718-9737 | MS.gene08869:intergenic | 40.0% | |
| GACGTAAACTTCGACGATTT+GGG | - | 32374:9626-9645 | MS.gene08869:intergenic | 40.0% | |
| ! | TAAACTTCGACGATTTGGGA+TGG | - | 32374:9622-9641 | MS.gene08869:intergenic | 40.0% |
| ATCTCCTGCTGCAAATCCTT+TGG | - | 32374:10049-10068 | MS.gene08869:intergenic | 45.0% | |
| CAAAATCTCCTTCACCGTTC+CGG | - | 32374:9983-10002 | MS.gene08869:intergenic | 45.0% | |
| CAAGGATTGGAAGGGTCTTA+GGG | + | 32374:9753-9772 | MS.gene08869:CDS | 45.0% | |
| CTGTTGGGTGTACTGTTGAT+GGG | + | 32374:10016-10035 | MS.gene08869:CDS | 45.0% | |
| CTTAGCCTGACGATTCTGAA+CGG | - | 32374:9794-9813 | MS.gene08869:intergenic | 45.0% | |
| GGACGTAAACTTCGACGATT+TGG | - | 32374:9627-9646 | MS.gene08869:intergenic | 45.0% | |
| GGTCTCTCACCAAAGAAGAT+CGG | + | 32374:9706-9725 | MS.gene08869:CDS | 45.0% | |
| TCTCCTGCTGCAAATCCTTT+GGG | - | 32374:10048-10067 | MS.gene08869:intergenic | 45.0% | |
| TCTGTTGGGTGTACTGTTGA+TGG | + | 32374:10015-10034 | MS.gene08869:CDS | 45.0% | |
| TTGCGAAGATTATGAAGCCG+AGG | + | 32374:9938-9957 | MS.gene08869:CDS | 45.0% | |
| ! | ATTTGGGATGGATCGAGCTT+TGG | - | 32374:9610-9629 | MS.gene08869:intergenic | 45.0% |
| ! | GAACGGTGAAGGAGATTTTG+GGG | + | 32374:9983-10002 | MS.gene08869:CDS | 45.0% |
| ! | GCTGGTTATCAAGGCTTTGA+AGG | + | 32374:9840-9859 | MS.gene08869:CDS | 45.0% |
| ! | GGAACGGTGAAGGAGATTTT+GGG | + | 32374:9982-10001 | MS.gene08869:CDS | 45.0% |
| ! | GTTGATGGGAAAGATCCCAA+AGG | + | 32374:10030-10049 | MS.gene08869:CDS | 45.0% |
| ! | TGGGGACTTGTGTTTCTGTT+GGG | + | 32374:10001-10020 | MS.gene08869:CDS | 45.0% |
| ! | TTGGGGACTTGTGTTTCTGT+TGG | + | 32374:10000-10019 | MS.gene08869:CDS | 45.0% |
| !! | AGGCTTTGAAGGAACCAGAA+AGG | + | 32374:9851-9870 | MS.gene08869:CDS | 45.0% |
| !! | GGCTTTGAAGGAACCAGAAA+GGG | + | 32374:9852-9871 | MS.gene08869:CDS | 45.0% |
| AAGACCCTTCCAATCCTTGG+CGG | - | 32374:9752-9771 | MS.gene08868:intergenic | 50.0% | |
| AGAAACCGCCAAGGATTGGA+AGG | + | 32374:9744-9763 | MS.gene08869:CDS | 50.0% | |
| CCAAAGAAACCGCCAAGGAT+TGG | + | 32374:9740-9759 | MS.gene08869:CDS | 50.0% | |
| CCAAGGATTGGAAGGGTCTT+AGG | + | 32374:9752-9771 | MS.gene08869:CDS | 50.0% | |
| CCAATCCTTGGCGGTTTCTT+TGG | - | 32374:9743-9762 | MS.gene08869:intergenic | 50.0% | |
| CCTAAGACCCTTCCAATCCT+TGG | - | 32374:9755-9774 | MS.gene08869:intergenic | 50.0% | |
| CGTTCAGAATCGTCAGGCTA+AGG | + | 32374:9792-9811 | MS.gene08869:CDS | 50.0% | |
| GAAACCGCCAAGGATTGGAA+GGG | + | 32374:9745-9764 | MS.gene08869:CDS | 50.0% | |
| GATCCCAAAGGATTTGCAGC+AGG | + | 32374:10042-10061 | MS.gene08869:CDS | 50.0% | |
| GATTATGAAGCCGAGGTCGA+TGG | + | 32374:9945-9964 | MS.gene08869:CDS | 50.0% | |
| TTCTTTGGTGAGAGACCGAG+AGG | - | 32374:9703-9722 | MS.gene08869:intergenic | 50.0% | |
| ! | CGGAACGGTGAAGGAGATTT+TGG | + | 32374:9981-10000 | MS.gene08869:CDS | 50.0% |
| AGACCGAGAGGACCGATCTT+GGG | - | 32374:9691-9710 | MS.gene08869:intergenic | 55.0% | |
| AGTTCCCTCGCTCCCAAGAT+CGG | + | 32374:9676-9695 | MS.gene08869:CDS | 55.0% | |
| CATCGCCAAAGAAACCGCCA+AGG | + | 32374:9735-9754 | MS.gene08869:CDS | 55.0% | |
| GAAGTTTACGTCCGCGTCAC+CGG | + | 32374:9634-9653 | MS.gene08869:CDS | 55.0% | |
| GCTCACCGTTCAGAATCGTC+AGG | + | 32374:9786-9805 | MS.gene08869:CDS | 55.0% | |
| GTCTTCTTGCGGTCCCTTTC+TGG | - | 32374:9868-9887 | MS.gene08869:intergenic | 55.0% | |
| ! | TGGGATGGATCGAGCTTTGG+CGG | - | 32374:9607-9626 | MS.gene08869:intergenic | 55.0% |
| AGCTCCTTCGCCATCGACCT+CGG | - | 32374:9958-9977 | MS.gene08869:intergenic | 60.0% | |
| AGGACCGATCTTGGGAGCGA+GGG | - | 32374:9683-9702 | MS.gene08869:intergenic | 60.0% | |
| GAGACCGAGAGGACCGATCT+TGG | - | 32374:9692-9711 | MS.gene08869:intergenic | 60.0% | |
| GATCTTGGGAGCGAGGGAAC+TGG | - | 32374:9677-9696 | MS.gene08869:intergenic | 60.0% | |
| GCTCCCAAGATCGGTCCTCT+CGG | + | 32374:9685-9704 | MS.gene08869:CDS | 60.0% | |
| GGTCGCTGTTGTTCCGTCTG+CGG | + | 32374:9813-9832 | MS.gene08869:CDS | 60.0% | |
| TCGATGGCGAAGGAGCTTGC+CGG | + | 32374:9961-9980 | MS.gene08869:CDS | 60.0% | |
| ATAACCAGCGCCGCCGCAGA+CGG | - | 32374:9829-9848 | MS.gene08869:intergenic | 65.0% | |
| CGCTGTTGTTCCGTCTGCGG+CGG | + | 32374:9816-9835 | MS.gene08869:CDS | 65.0% | |
| GAAGCCGAGGTCGATGGCGA+AGG | + | 32374:9951-9970 | MS.gene08869:CDS | 65.0% | |
| GAGGACCGATCTTGGGAGCG+AGG | - | 32374:9684-9703 | MS.gene08869:intergenic | 65.0% | |
| GGAGCTTGCCGGAACGGTGA+AGG | + | 32374:9972-9991 | MS.gene08869:CDS | 65.0% | |
| GGCGAAGGAGCTTGCCGGAA+CGG | + | 32374:9966-9985 | MS.gene08869:CDS | 65.0% | |
| GTTTACGTCCGCGTCACCGG+CGG | + | 32374:9637-9656 | MS.gene08869:CDS | 65.0% | |
| !! | TGCGGCGGCGCTGGTTATCA+AGG | + | 32374:9831-9850 | MS.gene08869:CDS | 65.0% |
| CGACTTCACCGCCGGTGACG+CGG | - | 32374:9648-9667 | MS.gene08869:intergenic | 70.0% | |
| CGCGTCACCGGCGGTGAAGT+CGG | + | 32374:9646-9665 | MS.gene08869:CDS | 70.0% | |
| GGCAGCTCCGACTTCACCGC+CGG | - | 32374:9656-9675 | MS.gene08869:intergenic | 70.0% | |
| TGTTCCGTCTGCGGCGGCGC+TGG | + | 32374:9822-9841 | MS.gene08869:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32374 | gene | 9601 | 10071 | 9601 | ID=MS.gene08869 |
| 32374 | mRNA | 9601 | 10071 | 9601 | ID=MS.gene08869.t1;Parent=MS.gene08869 |
| 32374 | exon | 9601 | 10071 | 9601 | ID=MS.gene08869.t1.exon1;Parent=MS.gene08869.t1 |
| 32374 | CDS | 9601 | 10071 | 9601 | ID=cds.MS.gene08869.t1;Parent=MS.gene08869.t1 |
| Gene Sequence |
| Protein sequence |
