Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26792.t1 | XP_013450365.1 | 100 | 188 | 0 | 0 | 1 | 188 | 1 | 188 | 7.90E-96 | 359.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26792.t1 | Q940B0 | 84.6 | 188 | 28 | 1 | 1 | 188 | 1 | 187 | 1.9e-82 | 306.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26792.t1 | A0A072U5K2 | 100.0 | 188 | 0 | 0 | 1 | 188 | 1 | 188 | 5.7e-96 | 359.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049306 | MS.gene26792 | 0.888329 | 6.50E-73 | -1.69E-46 |
| MS.gene049376 | MS.gene26792 | 0.81574 | 7.92E-52 | -1.69E-46 |
| MS.gene051925 | MS.gene26792 | 0.897406 | 1.45E-76 | -1.69E-46 |
| MS.gene051927 | MS.gene26792 | 0.897407 | 1.45E-76 | -1.69E-46 |
| MS.gene052666 | MS.gene26792 | 0.83865 | 2.54E-57 | -1.69E-46 |
| MS.gene05491 | MS.gene26792 | 0.809821 | 1.57E-50 | -1.69E-46 |
| MS.gene057321 | MS.gene26792 | 0.890989 | 5.98E-74 | -1.69E-46 |
| MS.gene057605 | MS.gene26792 | 0.809647 | 1.71E-50 | -1.69E-46 |
| MS.gene059340 | MS.gene26792 | 0.809279 | 2.05E-50 | -1.69E-46 |
| MS.gene060299 | MS.gene26792 | 0.80157 | 8.46E-49 | -1.69E-46 |
| MS.gene060483 | MS.gene26792 | 0.80069 | 1.28E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene40831 | MS.gene26792 | PPI |
| MS.gene025709 | MS.gene26792 | PPI |
| MS.gene22957 | MS.gene26792 | PPI |
| MS.gene06999 | MS.gene26792 | PPI |
| MS.gene26792 | MS.gene23126 | PPI |
| MS.gene23126 | MS.gene26792 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26792.t1 | MTR_7g108805 | 100.000 | 188 | 0 | 0 | 1 | 188 | 1 | 188 | 2.08e-134 | 374 |
| MS.gene26792.t1 | MTR_1g083460 | 91.489 | 188 | 15 | 1 | 1 | 188 | 1 | 187 | 1.02e-121 | 342 |
| MS.gene26792.t1 | MTR_7g112880 | 86.702 | 188 | 24 | 1 | 1 | 188 | 1 | 187 | 8.53e-117 | 329 |
| MS.gene26792.t1 | MTR_7g112880 | 84.444 | 135 | 20 | 1 | 54 | 188 | 1 | 134 | 1.17e-79 | 233 |
| MS.gene26792.t1 | MTR_4g005170 | 83.929 | 56 | 8 | 1 | 133 | 188 | 582 | 636 | 8.87e-24 | 97.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26792.t1 | AT5G27850 | 84.574 | 188 | 28 | 1 | 1 | 188 | 1 | 187 | 2.25e-113 | 320 |
| MS.gene26792.t1 | AT3G05590 | 83.511 | 188 | 30 | 1 | 1 | 188 | 1 | 187 | 3.60e-111 | 315 |
| MS.gene26792.t1 | AT2G47570 | 77.540 | 187 | 40 | 2 | 3 | 188 | 1 | 186 | 9.40e-101 | 288 |
| MS.gene26792.t1 | AT5G27850 | 82.963 | 135 | 22 | 1 | 54 | 188 | 1 | 134 | 1.19e-77 | 228 |
| MS.gene26792.t1 | AT3G05590 | 82.222 | 135 | 23 | 1 | 54 | 188 | 1 | 134 | 4.63e-77 | 227 |
| MS.gene26792.t1 | AT3G05590 | 82.222 | 135 | 23 | 1 | 54 | 188 | 1 | 134 | 4.63e-77 | 227 |
Find 57 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCGGTACGTTTAGTCTTCT+TGG | 0.192478 | 8.2:+10345386 | None:intergenic |
| TTATGAAGTACCTGCAATTA+AGG | 0.289178 | 8.2:-10345145 | MS.gene26792:CDS |
| CTGGCTCTTAGAGCTCCTTT+AGG | 0.297513 | 8.2:-10345045 | MS.gene26792:CDS |
| AGGTAGATATCATTGGATTT+TGG | 0.303386 | 8.2:+10345363 | None:intergenic |
| AGCCACCACTATCACTTTCT+AGG | 0.308293 | 8.2:-10345242 | MS.gene26792:CDS |
| TGGCTCTTAGAGCTCCTTTA+GGG | 0.321716 | 8.2:-10345044 | MS.gene26792:CDS |
| GTGCCGTGACAGATGACATT+AGG | 0.331949 | 8.2:-10345170 | MS.gene26792:CDS |
| GACTGAAAGTAATTTCAATA+AGG | 0.349360 | 8.2:-10345301 | MS.gene26792:CDS |
| AGAGCAGTTACCTTAATTGC+AGG | 0.362185 | 8.2:+10345135 | None:intergenic |
| TCAATAAGGTTATCCTTAAG+AGG | 0.371127 | 8.2:-10345287 | MS.gene26792:CDS |
| CAGAACACGGTTCTTCTTAG+AGG | 0.376334 | 8.2:-10345021 | MS.gene26792:CDS |
| TGCTCATGAAAAGCCTCTTA+AGG | 0.380870 | 8.2:+10345274 | None:intergenic |
| TTGAAGCCGCTAGAGGAAAA+AGG | 0.383923 | 8.2:-10344897 | MS.gene26792:CDS |
| TGCCGTGACAGATGACATTA+GGG | 0.403697 | 8.2:-10345169 | MS.gene26792:CDS |
| CAATTAAGGTAACTGCTCTA+AGG | 0.406837 | 8.2:-10345131 | MS.gene26792:CDS |
| AAGCTTGAGGTAGATATCAT+TGG | 0.411467 | 8.2:+10345356 | None:intergenic |
| GTGCCTCACCTTTGATCAGC+TGG | 0.415929 | 8.2:-10345064 | MS.gene26792:CDS |
| CACCATGGGTATCGATTTGA+AGG | 0.445042 | 8.2:-10345421 | None:intergenic |
| AATCTTACCCTCCTTTCCCT+TGG | 0.452146 | 8.2:+10345206 | None:intergenic |
| GGTAAGATTGCCGTAGTTGT+TGG | 0.465007 | 8.2:-10345192 | MS.gene26792:CDS |
| TGCTCTAAGGTTCACCGAAA+AGG | 0.469427 | 8.2:-10345118 | MS.gene26792:CDS |
| AAGCACTTTGGACGAGCTCC+TGG | 0.482073 | 8.2:-10344973 | MS.gene26792:CDS |
| CGTGAGGCTGTCAAGCACTT+TGG | 0.482505 | 8.2:-10344985 | MS.gene26792:CDS |
| TAAGGTTCACCGAAAAGGCC+AGG | 0.486544 | 8.2:-10345113 | MS.gene26792:CDS |
| AGCCTTCTCAATTCTAGCCC+TGG | 0.500353 | 8.2:+10345095 | None:intergenic |
| ATGGGTATCGATTTGAAGGC+AGG | 0.526453 | 8.2:-10345417 | MS.gene26792:CDS |
| CAGTCCTTCGAACAAGGAAA+CGG | 0.531384 | 8.2:+10345319 | None:intergenic |
| TACTTTCAGTCCTTCGAACA+AGG | 0.547564 | 8.2:+10345313 | None:intergenic |
| AGAACCGTGTTCTGCCCTAA+AGG | 0.548212 | 8.2:+10345030 | None:intergenic |
| ATCAACCTAGAAAGTGATAG+TGG | 0.548653 | 8.2:+10345237 | None:intergenic |
| CTAGAATTGAGAAGGCTGGT+GGG | 0.553032 | 8.2:-10345089 | MS.gene26792:CDS |
| GATTCGTTACACCAAGGGAA+AGG | 0.556958 | 8.2:-10345217 | MS.gene26792:CDS |
| AGGTTGATTCGTTACACCAA+GGG | 0.558600 | 8.2:-10345222 | MS.gene26792:CDS |
| AAAGTGCTTGACAGCCTCAC+GGG | 0.558822 | 8.2:+10344987 | None:intergenic |
| TATACCGTTTCCTTGTTCGA+AGG | 0.568847 | 8.2:-10345323 | MS.gene26792:CDS |
| GTGTTGCTGTGTGGAACACC+AGG | 0.574552 | 8.2:+10344955 | None:intergenic |
| AGGGCTAGAATTGAGAAGGC+TGG | 0.575107 | 8.2:-10345093 | MS.gene26792:CDS |
| CAAAGTGCTTGACAGCCTCA+CGG | 0.580659 | 8.2:+10344986 | None:intergenic |
| CGAAAGGTTGAAGCCGCTAG+AGG | 0.583684 | 8.2:-10344904 | MS.gene26792:CDS |
| GCTAGAATTGAGAAGGCTGG+TGG | 0.586828 | 8.2:-10345090 | MS.gene26792:CDS |
| TCGTTACACCAAGGGAAAGG+AGG | 0.587203 | 8.2:-10345214 | MS.gene26792:CDS |
| TCTAAGAGCCAGCTGATCAA+AGG | 0.589919 | 8.2:+10345056 | None:intergenic |
| TGCCTTCAAATCGATACCCA+TGG | 0.601064 | 8.2:+10345419 | None:intergenic |
| TAGAATTGAGAAGGCTGGTG+GGG | 0.601753 | 8.2:-10345088 | MS.gene26792:CDS |
| TGTCACGGCACCAACAACTA+CGG | 0.603846 | 8.2:+10345182 | None:intergenic |
| AGCTCCTTTAGGGCAGAACA+CGG | 0.606817 | 8.2:-10345034 | MS.gene26792:CDS |
| AACCCTAATGTCATCTGTCA+CGG | 0.612086 | 8.2:+10345167 | None:intergenic |
| GGCCAGGGCTAGAATTGAGA+AGG | 0.617622 | 8.2:-10345097 | MS.gene26792:CDS |
| AACACTAAGCCCTATGTCCG+TGG | 0.624133 | 8.2:-10344937 | MS.gene26792:CDS |
| AAGGTTCACCGAAAAGGCCA+GGG | 0.631182 | 8.2:-10345112 | MS.gene26792:CDS |
| CGTTACACCAAGGGAAAGGA+GGG | 0.633506 | 8.2:-10345213 | MS.gene26792:CDS |
| TAGGTTGATTCGTTACACCA+AGG | 0.646840 | 8.2:-10345223 | MS.gene26792:CDS |
| ATAGCTTAACGAGAAGCTTG+AGG | 0.649481 | 8.2:+10345343 | None:intergenic |
| AACCTAGAAAGTGATAGTGG+TGG | 0.668105 | 8.2:+10345240 | None:intergenic |
| GAGCCAGCTGATCAAAGGTG+AGG | 0.684975 | 8.2:+10345061 | None:intergenic |
| GGTATCGATTTGAAGGCAGG+AGG | 0.701621 | 8.2:-10345414 | MS.gene26792:CDS |
| TAGGGCTTAGTGTTGCTGTG+TGG | 0.715711 | 8.2:+10344946 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GACTGAAAGTAATTTCAATA+AGG | - | chr8.2:10344989-10345008 | MS.gene26792:CDS | 25.0% |
| TCAATAAGGTTATCCTTAAG+AGG | - | chr8.2:10345003-10345022 | MS.gene26792:CDS | 30.0% | |
| ! | AGGTAGATATCATTGGATTT+TGG | + | chr8.2:10344930-10344949 | None:intergenic | 30.0% |
| ! | TTATGAAGTACCTGCAATTA+AGG | - | chr8.2:10345145-10345164 | MS.gene26792:CDS | 30.0% |
| AAGCTTGAGGTAGATATCAT+TGG | + | chr8.2:10344937-10344956 | None:intergenic | 35.0% | |
| ATCAACCTAGAAAGTGATAG+TGG | + | chr8.2:10345056-10345075 | None:intergenic | 35.0% | |
| CAATTAAGGTAACTGCTCTA+AGG | - | chr8.2:10345159-10345178 | MS.gene26792:CDS | 35.0% | |
| !! | GATATCATTGGATTTTGGTG+CGG | + | chr8.2:10344925-10344944 | None:intergenic | 35.0% |
| !!! | CCTTTCGTGATTTTTTTCCA+CGG | + | chr8.2:10345373-10345392 | None:intergenic | 35.0% |
| !!! | GATTTTTTTCCACGGACATA+GGG | + | chr8.2:10345365-10345384 | None:intergenic | 35.0% |
| !!! | TGATTTTTTTCCACGGACAT+AGG | + | chr8.2:10345366-10345385 | None:intergenic | 35.0% |
| AACCCTAATGTCATCTGTCA+CGG | + | chr8.2:10345126-10345145 | None:intergenic | 40.0% | |
| AGAGCAGTTACCTTAATTGC+AGG | + | chr8.2:10345158-10345177 | None:intergenic | 40.0% | |
| ATAGCTTAACGAGAAGCTTG+AGG | + | chr8.2:10344950-10344969 | None:intergenic | 40.0% | |
| CCGTGGAAAAAAATCACGAA+AGG | - | chr8.2:10345370-10345389 | MS.gene26792:CDS | 40.0% | |
| TACTTTCAGTCCTTCGAACA+AGG | + | chr8.2:10344980-10344999 | None:intergenic | 40.0% | |
| TATACCGTTTCCTTGTTCGA+AGG | - | chr8.2:10344967-10344986 | MS.gene26792:CDS | 40.0% | |
| TGCTCATGAAAAGCCTCTTA+AGG | + | chr8.2:10345019-10345038 | None:intergenic | 40.0% | |
| !! | AACCTAGAAAGTGATAGTGG+TGG | + | chr8.2:10345053-10345072 | None:intergenic | 40.0% |
| !! | AGGTTGATTCGTTACACCAA+GGG | - | chr8.2:10345068-10345087 | MS.gene26792:CDS | 40.0% |
| !! | TAGGTTGATTCGTTACACCA+AGG | - | chr8.2:10345067-10345086 | MS.gene26792:CDS | 40.0% |
| AATCTTACCCTCCTTTCCCT+TGG | + | chr8.2:10345087-10345106 | None:intergenic | 45.0% | |
| AGAGGAAAAAGGAAGAGCAG+AGG | - | chr8.2:10345404-10345423 | MS.gene26792:CDS | 45.0% | |
| AGCCACCACTATCACTTTCT+AGG | - | chr8.2:10345048-10345067 | MS.gene26792:CDS | 45.0% | |
| CAGAACACGGTTCTTCTTAG+AGG | - | chr8.2:10345269-10345288 | MS.gene26792:CDS | 45.0% | |
| CAGTCCTTCGAACAAGGAAA+CGG | + | chr8.2:10344974-10344993 | None:intergenic | 45.0% | |
| CTAGAATTGAGAAGGCTGGT+GGG | - | chr8.2:10345201-10345220 | MS.gene26792:CDS | 45.0% | |
| GATTCGTTACACCAAGGGAA+AGG | - | chr8.2:10345073-10345092 | MS.gene26792:CDS | 45.0% | |
| GGTAAGATTGCCGTAGTTGT+TGG | - | chr8.2:10345098-10345117 | MS.gene26792:CDS | 45.0% | |
| TAGAATTGAGAAGGCTGGTG+GGG | - | chr8.2:10345202-10345221 | MS.gene26792:CDS | 45.0% | |
| TCTAAGAGCCAGCTGATCAA+AGG | + | chr8.2:10345237-10345256 | None:intergenic | 45.0% | |
| TGCCGTGACAGATGACATTA+GGG | - | chr8.2:10345121-10345140 | MS.gene26792:CDS | 45.0% | |
| TGGCTCTTAGAGCTCCTTTA+GGG | - | chr8.2:10345246-10345265 | MS.gene26792:CDS | 45.0% | |
| TTGAAGCCGCTAGAGGAAAA+AGG | - | chr8.2:10345393-10345412 | MS.gene26792:CDS | 45.0% | |
| ! | GCTCTTCCTTTTTCCTCTAG+CGG | + | chr8.2:10345402-10345421 | None:intergenic | 45.0% |
| ! | TGCGGTACGTTTAGTCTTCT+TGG | + | chr8.2:10344907-10344926 | None:intergenic | 45.0% |
| !! | AATTCTAGCCCTGGCCTTTT+CGG | + | chr8.2:10345189-10345208 | None:intergenic | 45.0% |
| !! | ATGGGTATCGATTTGAAGGC+AGG | - | chr8.2:10344873-10344892 | MS.gene26792:CDS | 45.0% |
| !! | TGCTCTAAGGTTCACCGAAA+AGG | - | chr8.2:10345172-10345191 | MS.gene26792:CDS | 45.0% |
| AACACTAAGCCCTATGTCCG+TGG | - | chr8.2:10345353-10345372 | MS.gene26792:CDS | 50.0% | |
| AAGGTTCACCGAAAAGGCCA+GGG | - | chr8.2:10345178-10345197 | MS.gene26792:CDS | 50.0% | |
| AGAACCGTGTTCTGCCCTAA+AGG | + | chr8.2:10345263-10345282 | None:intergenic | 50.0% | |
| AGCCTTCTCAATTCTAGCCC+TGG | + | chr8.2:10345198-10345217 | None:intergenic | 50.0% | |
| AGGGCTAGAATTGAGAAGGC+TGG | - | chr8.2:10345197-10345216 | MS.gene26792:CDS | 50.0% | |
| CGTTACACCAAGGGAAAGGA+GGG | - | chr8.2:10345077-10345096 | MS.gene26792:CDS | 50.0% | |
| GCTAGAATTGAGAAGGCTGG+TGG | - | chr8.2:10345200-10345219 | MS.gene26792:CDS | 50.0% | |
| GTGCCGTGACAGATGACATT+AGG | - | chr8.2:10345120-10345139 | MS.gene26792:CDS | 50.0% | |
| TAAGGTTCACCGAAAAGGCC+AGG | - | chr8.2:10345177-10345196 | MS.gene26792:CDS | 50.0% | |
| TCGTTACACCAAGGGAAAGG+AGG | - | chr8.2:10345076-10345095 | MS.gene26792:CDS | 50.0% | |
| TGTCACGGCACCAACAACTA+CGG | + | chr8.2:10345111-10345130 | None:intergenic | 50.0% | |
| ! | AAAGTGCTTGACAGCCTCAC+GGG | + | chr8.2:10345306-10345325 | None:intergenic | 50.0% |
| ! | ACAGCCTCACGGGCATTTTT+GGG | + | chr8.2:10345296-10345315 | None:intergenic | 50.0% |
| ! | CAAAGTGCTTGACAGCCTCA+CGG | + | chr8.2:10345307-10345326 | None:intergenic | 50.0% |
| ! | CTGGCTCTTAGAGCTCCTTT+AGG | - | chr8.2:10345245-10345264 | MS.gene26792:CDS | 50.0% |
| ! | TAGGGCTTAGTGTTGCTGTG+TGG | + | chr8.2:10345347-10345366 | None:intergenic | 50.0% |
| !! | AGCTCCTTTAGGGCAGAACA+CGG | - | chr8.2:10345256-10345275 | MS.gene26792:CDS | 50.0% |
| !! | GGTATCGATTTGAAGGCAGG+AGG | - | chr8.2:10344876-10344895 | MS.gene26792:CDS | 50.0% |
| AAGCACTTTGGACGAGCTCC+TGG | - | chr8.2:10345317-10345336 | MS.gene26792:CDS | 55.0% | |
| AGGTCCCAAAAATGCCCGTG+AGG | - | chr8.2:10345289-10345308 | MS.gene26792:CDS | 55.0% | |
| CGAAAGGTTGAAGCCGCTAG+AGG | - | chr8.2:10345386-10345405 | MS.gene26792:CDS | 55.0% | |
| CGTGAGGCTGTCAAGCACTT+TGG | - | chr8.2:10345305-10345324 | MS.gene26792:CDS | 55.0% | |
| GAGCCAGCTGATCAAAGGTG+AGG | + | chr8.2:10345232-10345251 | None:intergenic | 55.0% | |
| GGCCAGGGCTAGAATTGAGA+AGG | - | chr8.2:10345193-10345212 | MS.gene26792:CDS | 55.0% | |
| GTGCCTCACCTTTGATCAGC+TGG | - | chr8.2:10345226-10345245 | MS.gene26792:CDS | 55.0% | |
| ! | GACAGCCTCACGGGCATTTT+TGG | + | chr8.2:10345297-10345316 | None:intergenic | 55.0% |
| ! | GTGTTGCTGTGTGGAACACC+AGG | + | chr8.2:10345338-10345357 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 10344873 | 10345439 | 10344873 | ID=MS.gene26792 |
| chr8.2 | mRNA | 10344873 | 10345439 | 10344873 | ID=MS.gene26792.t1;Parent=MS.gene26792 |
| chr8.2 | exon | 10344873 | 10345439 | 10344873 | ID=MS.gene26792.t1.exon1;Parent=MS.gene26792.t1 |
| chr8.2 | CDS | 10344873 | 10345439 | 10344873 | ID=cds.MS.gene26792.t1;Parent=MS.gene26792.t1 |
| Gene Sequence |
| Protein sequence |
