AlfalfaGEDB | MS.gene067331

M. sativa cultivar XinJiangDaYe / MS.gene067331

KEGG: K02942
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006414 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0034645 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044249 GO:0044260 GO:0071704 GO:0110165 GO:1901576
PFAM: PF00428.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067331.t1	XP_013444548.1	100	60	0	0	1	60	1	60	7.90E-24	119.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067331.t1	Q8LCW9	61.7	60	23	0	2	61	3	62	6.1e-17	88.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067331.t1	I3S0D9	100.0	60	0	0	1	60	1	60	5.7e-24	119.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049306	MS.gene067331	0.806294	8.82E-50	-1.69E-46
MS.gene049361	MS.gene067331	0.820558	6.44E-53	-1.69E-46
MS.gene049387	MS.gene067331	0.862175	6.10E-64	-1.69E-46
MS.gene050134	MS.gene067331	0.889523	2.24E-73	-1.69E-46
MS.gene050252	MS.gene067331	0.817996	2.47E-52	-1.69E-46
MS.gene050638	MS.gene067331	-0.816122	6.51E-52	-1.69E-46
MS.gene050791	MS.gene067331	0.861397	1.06E-63	-1.69E-46
MS.gene050912	MS.gene067331	0.801484	8.81E-49	-1.69E-46
MS.gene051925	MS.gene067331	0.805959	1.04E-49	-1.69E-46
MS.gene051984	MS.gene067331	0.876834	1.02E-68	-1.69E-46
MS.gene052664	MS.gene067331	0.820718	5.92E-53	-1.69E-46
MS.gene052666	MS.gene067331	0.875713	2.48E-68	-1.69E-46
MS.gene053224	MS.gene067331	0.868228	7.63E-66	-1.69E-46
MS.gene054624	MS.gene067331	0.844612	6.79E-59	-1.69E-46
MS.gene057272	MS.gene067331	0.888066	8.21E-73	-1.69E-46
MS.gene057321	MS.gene067331	0.889374	2.56E-73	-1.69E-46
MS.gene057641	MS.gene067331	0.821943	3.09E-53	-1.69E-46
MS.gene057643	MS.gene067331	0.88115	3.05E-70	-1.69E-46
MS.gene058420	MS.gene067331	-0.801395	9.19E-49	-1.69E-46
MS.gene059340	MS.gene067331	0.865979	3.99E-65	-1.69E-46
MS.gene060299	MS.gene067331	0.818373	2.03E-52	-1.69E-46
MS.gene060483	MS.gene067331	0.96627	1.07E-125	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene067331	MS.gene053952	PPI
MS.gene067331	MS.gene01425	PPI
MS.gene067331	MS.gene032942	PPI
MS.gene82009	MS.gene067331	PPI
MS.gene073078	MS.gene067331	PPI
MS.gene069339	MS.gene067331	PPI
MS.gene067331	MS.gene35379	PPI
MS.gene067331	MS.gene01952	PPI
MS.gene067331	MS.gene88792	PPI
MS.gene067331	MS.gene047578	PPI
MS.gene009874	MS.gene067331	PPI
MS.gene44900	MS.gene067331	PPI
MS.gene067331	MS.gene06617	PPI
MS.gene067331	MS.gene002442	PPI
MS.gene067331	MS.gene01091	PPI
MS.gene067331	MS.gene78764	PPI
MS.gene43071	MS.gene067331	PPI
MS.gene08521	MS.gene067331	PPI
MS.gene067331	MS.gene045633	PPI
MS.gene067331	MS.gene057237	PPI
MS.gene042719	MS.gene067331	PPI
MS.gene06833	MS.gene067331	PPI
MS.gene067331	MS.gene060483	PPI
MS.gene013506	MS.gene067331	PPI
MS.gene76615	MS.gene067331	PPI
MS.gene067331	MS.gene91826	PPI
MS.gene067331	MS.gene97876	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067331.t1	MTR_8g024350	98.148	108	2	0	1	108	1	108	1.26e-72	211
MS.gene067331.t1	MTR_3g092600	60.000	110	42	1	1	108	1	110	4.73e-29	101
MS.gene067331.t1	MTR_2g066750	60.909	110	41	1	1	108	1	110	4.73e-29	101
MS.gene067331.t1	MTR_7g118060	66.102	59	20	0	1	59	1	59	1.53e-23	87.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067331.t1	AT5G24510	57.391	115	38	4	1	108	1	111	1.06e-37	123
MS.gene067331.t1	AT1G01100	51.402	107	39	1	2	108	3	96	7.04e-32	108
MS.gene067331.t1	AT4G00810	53.153	111	48	1	2	108	3	113	2.94e-31	107
MS.gene067331.t1	AT4G00810	53.153	111	48	1	2	108	3	113	2.94e-31	107
MS.gene067331.t1	AT1G01100	53.636	110	48	1	2	108	3	112	6.42e-31	106
MS.gene067331.t1	AT1G01100	53.636	110	48	1	2	108	3	112	6.42e-31	106
MS.gene067331.t1	AT1G01100	53.636	110	48	1	2	108	3	112	6.42e-31	106
MS.gene067331.t1	AT5G47700	53.153	111	48	1	2	108	3	113	9.37e-31	105
MS.gene067331.t1	AT5G47700	53.153	111	48	1	2	108	3	113	9.37e-31	105

Find 28 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGGAGGCAGCAGCAGCAAC+TGG	0.216487	8.4:-70690465	None:intergenic
ACGTTAATGTTGAAGCTTAC+TGG	0.234944	8.4:+70690349	MS.gene067331:CDS
TGGTGCTCCGGTTTCTGTTT+CGG	0.321080	8.4:+70690440	MS.gene067331:CDS
GTCGTCGTCACTCTCATCCT+TGG	0.357062	8.4:-70690615	None:intergenic
ATCATCAAGGTTCTTCTTCT+CGG	0.364681	8.4:-70690394	None:intergenic
ACTGGGGCCGAAACAGAAAC+CGG	0.462684	8.4:-70690447	None:intergenic
TCGTCGTCACTCTCATCCTT+GGG	0.464875	8.4:-70690614	None:intergenic
TGCCGCTGGAGGTGGTGCTC+CGG	0.491299	8.4:+70690428	MS.gene067331:CDS
ATATTGAATGCCGCTGGAGG+TGG	0.496313	8.4:+70690420	MS.gene067331:CDS
GCAAGTTTAGCGAATAAACT+TGG	0.520693	8.4:-70690372	None:intergenic
TGCTCCTATCGAGGAGAAGA+AGG	0.520970	8.4:+70690497	MS.gene067331:CDS
TGGAGGCAGCAGCAGCAACT+GGG	0.544694	8.4:-70690464	None:intergenic
AGGAGCAGCAACTGCAGTGG+AGG	0.545209	8.4:-70690481	None:intergenic
GGATGAGAGTGACGACGACA+TGG	0.547177	8.4:+70690619	MS.gene067331:CDS
GTTTGCAGCTTTCAACAAAG+TGG	0.560297	8.4:-70690328	None:intergenic
GATTTGATATTGAATGCCGC+TGG	0.580711	8.4:+70690414	MS.gene067331:CDS
AACCGGAGCACCACCTCCAG+CGG	0.583475	8.4:-70690430	None:intergenic
CGGCGATGTTATCATCATGA+AGG	0.588233	8.4:-70689612	None:intergenic
CATTCAATATCAAATCATCA+AGG	0.592309	8.4:-70690407	None:intergenic
TGCAGTTGCTGCTCCTATCG+AGG	0.607768	8.4:+70690488	MS.gene067331:CDS
GGAGGCAGCAGCAGCAACTG+GGG	0.621755	8.4:-70690463	None:intergenic
CCTACCTTCTTCTCCTCGAT+AGG	0.622145	8.4:-70690501	None:intergenic
GATAGGAGCAGCAACTGCAG+TGG	0.632714	8.4:-70690484	None:intergenic
TGTAGTGTGAAAATGTCGAT+CGG	0.636502	8.4:+70689559	None:intergenic
ATTATCAAAGGATACAGTGA+CGG	0.653291	8.4:-70689632	None:intergenic
GATGAGAGTGACGACGACAT+GGG	0.653294	8.4:+70690620	MS.gene067331:CDS
GTACTTACAGGAAGAACCCA+AGG	0.654187	8.4:+70690598	MS.gene067331:intron
TTGATATTGAATGCCGCTGG+AGG	0.659375	8.4:+70690417	MS.gene067331:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAAAAATTAGGTTTTTTAA+AGG	+	chr8.4:70690022-70690041	MS.gene067331:intron	10.0%
!!!	AAAATCAATTTTTATATAGT+GGG	+	chr8.4:70690173-70690192	MS.gene067331:intron	10.0%
!!!	ATTATTACTGAATTTTTTAT+TGG	+	chr8.4:70689892-70689911	MS.gene067331:intron	10.0%
!!!	TAAAAAACCTAATTTTTTTA+AGG	-	chr8.4:70690021-70690040	None:intergenic	10.0%
!!!	TAAAATCAATTTTTATATAG+TGG	+	chr8.4:70690172-70690191	MS.gene067331:intron	10.0%
!!	AAAAATTCAGTAATAATTTG+AGG	-	chr8.4:70689889-70689908	None:intergenic	15.0%
!!	AATTAGTCCTTAAAAAAATT+AGG	+	chr8.4:70690011-70690030	MS.gene067331:intron	15.0%
!!	ATTACATTATCAATTATCAA+AGG	-	chr8.4:70689647-70689666	None:intergenic	15.0%
!!!	AAAAATTGATTTTACAACTT+TGG	-	chr8.4:70690166-70690185	None:intergenic	15.0%
!!!	TGAAAAAAAAGAGATTTTTT+TGG	+	chr8.4:70689740-70689759	MS.gene067331:intron	15.0%
!!!	ACTAAATCAACAATTTTCTA+CGG	+	chr8.4:70690133-70690152	MS.gene067331:intron	20.0%
!!!	TCTTATCTAATTGGTATTTT+TGG	+	chr8.4:70690573-70690592	MS.gene067331:intron	20.0%
!	AATGACAATTAACAACTCTT+AGG	-	chr8.4:70689970-70689989	None:intergenic	25.0%
!	CATTCAATATCAAATCATCA+AGG	-	chr8.4:70690410-70690429	None:intergenic	25.0%
!	TAATGAATTTAGAAGATGCT+AGG	+	chr8.4:70689707-70689726	MS.gene067331:intron	25.0%
!!!	AGATTTTTTTGGATGATACA+AGG	+	chr8.4:70689751-70689770	MS.gene067331:intron	25.0%
!!!	ATTTTTATATAGTGGGAATC+TGG	+	chr8.4:70690180-70690199	MS.gene067331:intron	25.0%
!!!	ATTTTTTTGGATGATACAAG+GGG	+	chr8.4:70689753-70689772	MS.gene067331:intron	25.0%
!!!	GAATTTTGTTTTGGTGAATA+AGG	+	chr8.4:70690208-70690227	MS.gene067331:intron	25.0%
!!!	GATTTTTTTGGATGATACAA+GGG	+	chr8.4:70689752-70689771	MS.gene067331:intron	25.0%
!!!	TTTTTATATAGTGGGAATCT+GGG	+	chr8.4:70690181-70690200	MS.gene067331:intron	25.0%
	ATTATCAAAGGATACAGTGA+CGG	-	chr8.4:70689635-70689654	None:intergenic	30.0%
	ATTTAGAAGATGCTAGGAAT+TGG	+	chr8.4:70689713-70689732	MS.gene067331:intron	30.0%
	TAAAGTTCTTGGATAGAGTT+AGG	+	chr8.4:70690071-70690090	MS.gene067331:intron	30.0%
	TAAGTGCTAGTTAAAGTTCT+TGG	+	chr8.4:70690060-70690079	MS.gene067331:intron	30.0%
	TCCTTAAGTCATTGTTGATA+GGG	+	chr8.4:70690294-70690313	MS.gene067331:intron	30.0%
	TGTTGGTCATCTTATCTAAT+TGG	+	chr8.4:70690564-70690583	MS.gene067331:intron	30.0%
	TTTAGAAGATGCTAGGAATT+GGG	+	chr8.4:70689714-70689733	MS.gene067331:intron	30.0%
!	ATCAACAATTTTCTACGGTA+AGG	+	chr8.4:70690138-70690157	MS.gene067331:intron	30.0%
!	TCAACAATTTTCTACGGTAA+GGG	+	chr8.4:70690139-70690158	MS.gene067331:intron	30.0%
!!!	AGATAGCGTGTTTTAAAGAT+TGG	+	chr8.4:70690267-70690286	MS.gene067331:intron	30.0%
!!!	CTGGGTTTTGAATTTTGTTT+TGG	+	chr8.4:70690199-70690218	MS.gene067331:intron	30.0%
!!!	GTATTTTTGGTTGTACTTAC+AGG	+	chr8.4:70690586-70690605	MS.gene067331:intron	30.0%
	ACCCTATCAACAATGACTTA+AGG	-	chr8.4:70690298-70690317	None:intergenic	35.0%
	ACGTTAATGTTGAAGCTTAC+TGG	+	chr8.4:70690349-70690368	MS.gene067331:CDS	35.0%
	ATCATCAAGGTTCTTCTTCT+CGG	-	chr8.4:70690397-70690416	None:intergenic	35.0%
	CTCCTTAAGTCATTGTTGAT+AGG	+	chr8.4:70690293-70690312	MS.gene067331:intron	35.0%
	GAACTTTGTCTTCTGTTTGT+TGG	+	chr8.4:70690547-70690566	MS.gene067331:intron	35.0%
	GCAAGTTTAGCGAATAAACT+TGG	-	chr8.4:70690375-70690394	None:intergenic	35.0%
	TTTGGTGAATAAGGAAAGTG+TGG	+	chr8.4:70690217-70690236	MS.gene067331:intron	35.0%
!	AATAAGGAAAGTGTGGTGTA+AGG	+	chr8.4:70690224-70690243	MS.gene067331:intron	35.0%
!	ATAAGGAAAGTGTGGTGTAA+GGG	+	chr8.4:70690225-70690244	MS.gene067331:intron	35.0%
	GATGATACAAGGGGCTATAT+AGG	+	chr8.4:70689762-70689781	MS.gene067331:intron	40.0%
	GATTTGATATTGAATGCCGC+TGG	+	chr8.4:70690414-70690433	MS.gene067331:CDS	40.0%
	GTTTGCAGCTTTCAACAAAG+TGG	-	chr8.4:70690331-70690350	None:intergenic	40.0%
!	AGGAGAAGAAGGTAGGTTTT+TGG	+	chr8.4:70690508-70690527	MS.gene067331:intron	40.0%
!!!	TCGATCAGAAGTGTCGTTTT+AGG	-	chr8.4:70689796-70689815	None:intergenic	40.0%
	CGGCGATGTTATCATCATGA+AGG	-	chr8.4:70689615-70689634	None:intergenic	45.0%
	CTTCTGATCGAAGTTGTGTC+TGG	+	chr8.4:70689805-70689824	MS.gene067331:intron	45.0%
	GTACTTACAGGAAGAACCCA+AGG	+	chr8.4:70690598-70690617	MS.gene067331:intron	45.0%
	TTGATATTGAATGCCGCTGG+AGG	+	chr8.4:70690417-70690436	MS.gene067331:CDS	45.0%
!!!	TTTTTTTATTTTCTTATTTT+AGG	+	chr8.4:70690110-70690129	MS.gene067331:intron	5.0%
	ATATTGAATGCCGCTGGAGG+TGG	+	chr8.4:70690420-70690439	MS.gene067331:CDS	50.0%
	CCTACCTTCTTCTCCTCGAT+AGG	-	chr8.4:70690504-70690523	None:intergenic	50.0%
	CCTATCGAGGAGAAGAAGGT+AGG	+	chr8.4:70690501-70690520	MS.gene067331:intron	50.0%
	GATGAGAGTGACGACGACAT+GGG	+	chr8.4:70690620-70690639	MS.gene067331:CDS	50.0%
	TCGTCGTCACTCTCATCCTT+GGG	-	chr8.4:70690617-70690636	None:intergenic	50.0%
	TGCTCCTATCGAGGAGAAGA+AGG	+	chr8.4:70690497-70690516	MS.gene067331:CDS	50.0%
	TGGTGCTCCGGTTTCTGTTT+CGG	+	chr8.4:70690440-70690459	MS.gene067331:CDS	50.0%
	TGGTGTGTGTCAGTGCTTCA+TGG	+	chr8.4:70689912-70689931	MS.gene067331:intron	50.0%
	ACTGGGGCCGAAACAGAAAC+CGG	-	chr8.4:70690450-70690469	None:intergenic	55.0%
	GATAGGAGCAGCAACTGCAG+TGG	-	chr8.4:70690487-70690506	None:intergenic	55.0%
	GGATGAGAGTGACGACGACA+TGG	+	chr8.4:70690619-70690638	MS.gene067331:CDS	55.0%
	GTCGTCGTCACTCTCATCCT+TGG	-	chr8.4:70690618-70690637	None:intergenic	55.0%
	TGCAGTTGCTGCTCCTATCG+AGG	+	chr8.4:70690488-70690507	MS.gene067331:CDS	55.0%
	AGGAGCAGCAACTGCAGTGG+AGG	-	chr8.4:70690484-70690503	None:intergenic	60.0%
	TGGAGGCAGCAGCAGCAACT+GGG	-	chr8.4:70690467-70690486	None:intergenic	60.0%
	GGAGGCAGCAGCAGCAACTG+GGG	-	chr8.4:70690466-70690485	None:intergenic	65.0%
	GTGGAGGCAGCAGCAGCAAC+TGG	-	chr8.4:70690468-70690487	None:intergenic	65.0%
!	AACCGGAGCACCACCTCCAG+CGG	-	chr8.4:70690433-70690452	None:intergenic	65.0%
	TGCCGCTGGAGGTGGTGCTC+CGG	+	chr8.4:70690428-70690447	MS.gene067331:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	70689571	70690661	70689571	ID=MS.gene067331
chr8.4	mRNA	70689571	70690661	70689571	ID=MS.gene067331.t1;Parent=MS.gene067331
chr8.4	exon	70689571	70689639	70689571	ID=MS.gene067331.t1.exon1;Parent=MS.gene067331.t1
chr8.4	CDS	70689571	70689639	70689571	ID=cds.MS.gene067331.t1;Parent=MS.gene067331.t1
chr8.4	exon	70690315	70690518	70690315	ID=MS.gene067331.t1.exon2;Parent=MS.gene067331.t1
chr8.4	CDS	70690315	70690518	70690315	ID=cds.MS.gene067331.t1;Parent=MS.gene067331.t1
chr8.4	exon	70690608	70690661	70690608	ID=MS.gene067331.t1.exon3;Parent=MS.gene067331.t1
chr8.4	CDS	70690608	70690661	70690608	ID=cds.MS.gene067331.t1;Parent=MS.gene067331.t1

Gene Sequence

>MS.gene067331

ATGTCGATCGGAGAAGTTGCTTGCAGCTACTCACTTATGATCCTTCATGATGATAACATCGCCGTCACTGTATCCTTTGATAATTGATAATGTAATTAATTTAACTATGATTTTCTTTTGTTAGTATATGTTTTTTTAATGAATTTAGAAGATGCTAGGAATTGGGTTTTGAAAAAAAAGAGATTTTTTTGGATGATACAAGGGGCTATATAGGATTATGAACCTAAAACGACACTTCTGATCGAAGTTGTGTCTGGTGTCTTTTGCGTGTTTTGTCAGCGCACTCACATTCAATTACATTTGATTAATTCATTTCCTCAAATTATTACTGAATTTTTTATTGGTGTGTGTCAGTGCTTCATGGTCTCTGATGCAAGTGAAATTCAGAATATAATTCCTAAGAGTTGTTAATTGTCATTTAGATTCAATTATTGAAATCAAATTAGTCCTTAAAAAAATTAGGTTTTTTAAAGGTTAAACTAGTTTTGATAAGTGCTAGTTAAAGTTCTTGGATAGAGTTAGGATAATGTTTTTGTGATTTTTTTTATTTTCTTATTTTAGGACTAAATCAACAATTTTCTACGGTAAGGGACCAAAGTTGTAAAATCAATTTTTATATAGTGGGAATCTGGGTTTTGAATTTTGTTTTGGTGAATAAGGAAAGTGTGGTGTAAGGGTTTGAAGTTGAATATTTGAAGATAGCGTGTTTTAAAGATTGGTTTCTCCTTAAGTCATTGTTGATAGGGTGAGAAAATCTCCACTTTGTTGAAAGCTGCAAACGTTAATGTTGAAGCTTACTGGCCAAGTTTATTCGCTAAACTTGCCGAGAAGAAGAACCTTGATGATTTGATATTGAATGCCGCTGGAGGTGGTGCTCCGGTTTCTGTTTCGGCCCCAGTTGCTGCTGCTGCCTCCACTGCAGTTGCTGCTCCTATCGAGGAGAAGAAGGTAGGTTTTTGGTGAAAAAGTTGTTGTTGAACTTTGTCTTCTGTTTGTTGGTCATCTTATCTAATTGGTATTTTTGGTTGTACTTACAGGAAGAACCCAAGGATGAGAGTGACGACGACATGGGATTCAGCTTGTTTGATTAG

Protein sequence

>MS.gene067331.t1

MSIGEVACSYSLMILHDDNIAVTGEKISTLLKAANVNVEAYWPSLFAKLAEKKNLDDLILNAAGGGAPVSVSAPVAAAASTAVAAPIEEKKEEPKDESDDDMGFSLFD