Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060424.t1 | KEH18058.1 | 67.9 | 156 | 31 | 3 | 3 | 153 | 146 | 287 | 4.00E-45 | 191 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060424.t1 | A0A072TMI0 | 67.9 | 156 | 31 | 3 | 3 | 153 | 146 | 287 | 2.9e-45 | 191.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060424 | MS.gene069828 | 0.801827 | 7.49E-49 | -1.69E-46 |
| MS.gene060424 | MS.gene29794 | 0.805273 | 1.45E-49 | -1.69E-46 |
| MS.gene060424 | MS.gene41088 | 0.800309 | 1.53E-48 | -1.69E-46 |
| MS.gene060424 | MS.gene45537 | 0.807598 | 4.67E-50 | -1.69E-46 |
| MS.gene060424 | MS.gene86061 | 0.82339 | 1.42E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060424.t1 | MTR_8g010370 | 67.949 | 156 | 31 | 3 | 3 | 153 | 146 | 287 | 8.03e-65 | 199 |
| MS.gene060424.t1 | MTR_2g104470 | 85.714 | 112 | 11 | 1 | 47 | 153 | 24 | 135 | 8.01e-64 | 192 |
| MS.gene060424.t1 | MTR_4g017520 | 85.185 | 108 | 11 | 1 | 47 | 149 | 24 | 131 | 3.48e-61 | 188 |
| MS.gene060424.t1 | MTR_1g062200 | 58.228 | 79 | 20 | 2 | 1 | 79 | 108 | 173 | 9.10e-20 | 85.1 |
| MS.gene060424.t1 | MTR_1g062200 | 58.228 | 79 | 20 | 2 | 1 | 79 | 108 | 173 | 9.16e-20 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGAAACAAATCTCATTTAA+AGG | 0.268953 | 7.1:-66350502 | MS.gene060424:CDS |
| AATTCTCTGGCGGCCGAAAA+TGG | 0.315874 | 7.1:-66349836 | MS.gene060424:CDS |
| ACCATAGTTTCAACTTCTTC+AGG | 0.316823 | 7.1:+66350369 | None:intergenic |
| ATAATTGCTCTTAGTTCATC+TGG | 0.365914 | 7.1:+66349799 | None:intergenic |
| ACCTGAAGAAGTTGAAACTA+TGG | 0.384871 | 7.1:-66350370 | MS.gene060424:intron |
| CCATGGTTGCACTTATTTAG+TGG | 0.392399 | 7.1:+66350886 | None:intergenic |
| TATGAGTGGGTTGTGTTATG+AGG | 0.401994 | 7.1:-66350230 | MS.gene060424:CDS |
| TTCAACTTCTTCAGGTCATA+AGG | 0.405343 | 7.1:+66350377 | None:intergenic |
| AGTGTTGACTCTTCCTCTCT+TGG | 0.422612 | 7.1:-66350414 | MS.gene060424:CDS |
| CATGGTTGCACTTATTTAGT+GGG | 0.430381 | 7.1:+66350887 | None:intergenic |
| CTCTTCCTCTCTTGGTGATG+TGG | 0.455409 | 7.1:-66350406 | MS.gene060424:CDS |
| GAATTTCAAACTCTGTTCAA+TGG | 0.461673 | 7.1:+66350658 | None:intergenic |
| TGAATCTGAACTCGCGTAGC+TGG | 0.475898 | 7.1:+66350927 | None:intergenic |
| TTATTTAGTGGGATTGAAAG+TGG | 0.496505 | 7.1:+66350898 | None:intergenic |
| CTTAGTTCATCTGGCTCCAT+CGG | 0.502803 | 7.1:+66349808 | None:intergenic |
| TGAGGGCTACTTACATATCC+AGG | 0.532818 | 7.1:-66350212 | MS.gene060424:intron |
| AAGTTACTGTCAAGTCAGAT+AGG | 0.537870 | 7.1:-66350458 | MS.gene060424:intron |
| GAACAGAGTTTGAAATTCAG+TGG | 0.548164 | 7.1:-66350653 | MS.gene060424:CDS |
| ACATTAGTAAAAGAATTCTC+TGG | 0.553548 | 7.1:-66349849 | MS.gene060424:CDS |
| ATGAGTGGGTTGTGTTATGA+GGG | 0.554083 | 7.1:-66350229 | MS.gene060424:CDS |
| TTTCAGGGATCAAGAATCAA+GGG | 0.560098 | 7.1:-66350522 | MS.gene060424:intron |
| GCCGAAAATGGTATCACCGA+TGG | 0.562882 | 7.1:-66349824 | MS.gene060424:CDS |
| CCACTAAATAAGTGCAACCA+TGG | 0.572318 | 7.1:-66350886 | MS.gene060424:CDS |
| AGAGTCCACATCACCAAGAG+AGG | 0.586110 | 7.1:+66350401 | None:intergenic |
| AGGTTGCGAGATTATATGAG+TGG | 0.605845 | 7.1:-66350244 | MS.gene060424:CDS |
| TCACATTGAAAATTGTCCCA+TGG | 0.611171 | 7.1:+66350869 | None:intergenic |
| CACTAAATAAGTGCAACCAT+GGG | 0.612855 | 7.1:-66350885 | MS.gene060424:CDS |
| GGTTGCGAGATTATATGAGT+GGG | 0.681349 | 7.1:-66350243 | MS.gene060424:CDS |
| TTAGTAAAAGAATTCTCTGG+CGG | 0.698230 | 7.1:-66349846 | MS.gene060424:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTGAAAATATACAAAAAT+TGG | + | chr7.1:66350766-66350785 | None:intergenic | 15.0% |
| !! | AAAAATAAAATAAGCTGAGA+TGG | + | chr7.1:66350824-66350843 | None:intergenic | 20.0% |
| !! | AAAAATGATGTAAAGAAATC+AGG | + | chr7.1:66350457-66350476 | None:intergenic | 20.0% |
| !! | ATAGTATTATGTGTATAGTA+AGG | - | chr7.1:66349990-66350009 | MS.gene060424:intron | 20.0% |
| !! | CTATATATTATGTGCATAAA+AGG | - | chr7.1:66349893-66349912 | MS.gene060424:intron | 20.0% |
| ! | AAACTATGGTAAAATTGCAA+AGG | - | chr7.1:66350367-66350386 | MS.gene060424:intron | 25.0% |
| ! | ACATTAGTAAAAGAATTCTC+TGG | - | chr7.1:66350874-66350893 | MS.gene060424:CDS | 25.0% |
| ! | AGATATACACGTATGTATTT+AGG | - | chr7.1:66350018-66350037 | MS.gene060424:intron | 25.0% |
| ! | CACCATTAAAATCATTAAAG+AGG | + | chr7.1:66350603-66350622 | None:intergenic | 25.0% |
| ! | GATATTTATGAGTTTAGATG+TGG | - | chr7.1:66350391-66350410 | MS.gene060424:CDS | 25.0% |
| ! | TAAAGAAATCAGGAAAATGA+AGG | + | chr7.1:66350447-66350466 | None:intergenic | 25.0% |
| !! | GCATTTTCTGTCAAATTTTA+GGG | - | chr7.1:66349915-66349934 | MS.gene060424:intron | 25.0% |
| !! | TGCCTCTTTAATGATTTTAA+TGG | - | chr7.1:66350598-66350617 | MS.gene060424:intron | 25.0% |
| !!! | TTATTTTTTTCAGCACATCA+TGG | - | chr7.1:66350836-66350855 | MS.gene060424:intron | 25.0% |
| !!! | TTTCTTTACATCATTTTTGC+AGG | - | chr7.1:66350459-66350478 | MS.gene060424:CDS | 25.0% |
| AAAAATAATAGAAGCTGACC+TGG | + | chr7.1:66350532-66350551 | None:intergenic | 30.0% | |
| AAGAACCAGCATATTGTAAA+CGG | + | chr7.1:66350667-66350686 | None:intergenic | 30.0% | |
| AATAAAATAAGCTGAGATGG+TGG | + | chr7.1:66350821-66350840 | None:intergenic | 30.0% | |
| ATAATTGCTCTTAGTTCATC+TGG | + | chr7.1:66350927-66350946 | None:intergenic | 30.0% | |
| ATACACGTATGTATTTAGGT+AGG | - | chr7.1:66350022-66350041 | MS.gene060424:intron | 30.0% | |
| GAATTTCAAACTCTGTTCAA+TGG | + | chr7.1:66350068-66350087 | None:intergenic | 30.0% | |
| GGGAAACAAATCTCATTTAA+AGG | - | chr7.1:66350221-66350240 | MS.gene060424:CDS | 30.0% | |
| GTTTCTTTGTAGATGATCTT+AGG | - | chr7.1:66350573-66350592 | MS.gene060424:intron | 30.0% | |
| TTACACCAAGAAAAAAATCC+AGG | - | chr7.1:66350096-66350115 | MS.gene060424:intron | 30.0% | |
| TTAGTAAAAGAATTCTCTGG+CGG | - | chr7.1:66350877-66350896 | MS.gene060424:CDS | 30.0% | |
| TTATTTAGTGGGATTGAAAG+TGG | + | chr7.1:66349828-66349847 | None:intergenic | 30.0% | |
| ! | GGCATTTTCTGTCAAATTTT+AGG | - | chr7.1:66349914-66349933 | MS.gene060424:intron | 30.0% |
| ! | TGATCTCTTGCAAATTTTCA+GGG | - | chr7.1:66350186-66350205 | MS.gene060424:intron | 30.0% |
| !!! | ACAAACCTGGATTTTTTTCT+TGG | + | chr7.1:66350104-66350123 | None:intergenic | 30.0% |
| !!! | GGTCAGCTTCTATTATTTTT+TGG | - | chr7.1:66350532-66350551 | MS.gene060424:intron | 30.0% |
| AAAATCCAGGTTTGTATCGA+AGG | - | chr7.1:66350109-66350128 | MS.gene060424:intron | 35.0% | |
| AAATCCAGGTTTGTATCGAA+GGG | - | chr7.1:66350110-66350129 | MS.gene060424:intron | 35.0% | |
| AAGTTACTGTCAAGTCAGAT+AGG | - | chr7.1:66350265-66350284 | MS.gene060424:intron | 35.0% | |
| ACCATAGTTTCAACTTCTTC+AGG | + | chr7.1:66350357-66350376 | None:intergenic | 35.0% | |
| ACCTGAAGAAGTTGAAACTA+TGG | - | chr7.1:66350353-66350372 | MS.gene060424:intron | 35.0% | |
| ATATTGTAAACGGAATGAGC+TGG | + | chr7.1:66350657-66350676 | None:intergenic | 35.0% | |
| CACTAAATAAGTGCAACCAT+GGG | - | chr7.1:66349838-66349857 | MS.gene060424:CDS | 35.0% | |
| CATGGTTGCACTTATTTAGT+GGG | + | chr7.1:66349839-66349858 | None:intergenic | 35.0% | |
| GGTTGTAAAATGTCTTGCTT+TGG | - | chr7.1:66350412-66350431 | MS.gene060424:CDS | 35.0% | |
| TCACATTGAAAATTGTCCCA+TGG | + | chr7.1:66349857-66349876 | None:intergenic | 35.0% | |
| TCATTCCGTTTACAATATGC+TGG | - | chr7.1:66350659-66350678 | MS.gene060424:CDS | 35.0% | |
| TTCAACTTCTTCAGGTCATA+AGG | + | chr7.1:66350349-66350368 | None:intergenic | 35.0% | |
| TTTCAGGGATCAAGAATCAA+GGG | - | chr7.1:66350201-66350220 | MS.gene060424:intron | 35.0% | |
| ! | GAACAGAGTTTGAAATTCAG+TGG | - | chr7.1:66350070-66350089 | MS.gene060424:intron | 35.0% |
| ! | GTGATCTCTTGCAAATTTTC+AGG | - | chr7.1:66350185-66350204 | MS.gene060424:intron | 35.0% |
| !! | TTTTCAGGGATCAAGAATCA+AGG | - | chr7.1:66350200-66350219 | MS.gene060424:intron | 35.0% |
| AGGTTGCGAGATTATATGAG+TGG | - | chr7.1:66350479-66350498 | MS.gene060424:CDS | 40.0% | |
| CCACTAAATAAGTGCAACCA+TGG | - | chr7.1:66349837-66349856 | MS.gene060424:CDS | 40.0% | |
| CCATGGTTGCACTTATTTAG+TGG | + | chr7.1:66349840-66349859 | None:intergenic | 40.0% | |
| GGTTGCGAGATTATATGAGT+GGG | - | chr7.1:66350480-66350499 | MS.gene060424:CDS | 40.0% | |
| ! | TCCATCGGTGATACCATTTT+CGG | + | chr7.1:66350903-66350922 | None:intergenic | 40.0% |
| !! | ATGAGTGGGTTGTGTTATGA+GGG | - | chr7.1:66350494-66350513 | MS.gene060424:CDS | 40.0% |
| !! | TATGAGTGGGTTGTGTTATG+AGG | - | chr7.1:66350493-66350512 | MS.gene060424:CDS | 40.0% |
| ATCACCCTTCGATACAAACC+TGG | + | chr7.1:66350117-66350136 | None:intergenic | 45.0% | |
| TGAGGGCTACTTACATATCC+AGG | - | chr7.1:66350511-66350530 | MS.gene060424:CDS | 45.0% | |
| ! | CTTAGTTCATCTGGCTCCAT+CGG | + | chr7.1:66350918-66350937 | None:intergenic | 45.0% |
| !! | AGTGTTGACTCTTCCTCTCT+TGG | - | chr7.1:66350309-66350328 | MS.gene060424:intron | 45.0% |
| !! | ACATTATAATTATTATATAA+AGG | + | chr7.1:66350140-66350159 | None:intergenic | 5.0% |
| AATTCTCTGGCGGCCGAAAA+TGG | - | chr7.1:66350887-66350906 | MS.gene060424:CDS | 50.0% | |
| AGAGTCCACATCACCAAGAG+AGG | + | chr7.1:66350325-66350344 | None:intergenic | 50.0% | |
| GCCGAAAATGGTATCACCGA+TGG | - | chr7.1:66350899-66350918 | MS.gene060424:CDS | 50.0% | |
| TGAATCTGAACTCGCGTAGC+TGG | + | chr7.1:66349799-66349818 | None:intergenic | 50.0% | |
| !! | CTCTTCCTCTCTTGGTGATG+TGG | - | chr7.1:66350317-66350336 | MS.gene060424:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 66349792 | 66350953 | 66349792 | ID=MS.gene060424 |
| chr7.1 | mRNA | 66349792 | 66350953 | 66349792 | ID=MS.gene060424.t1;Parent=MS.gene060424 |
| chr7.1 | exon | 66350873 | 66350953 | 66350873 | ID=MS.gene060424.t1.exon1;Parent=MS.gene060424.t1 |
| chr7.1 | CDS | 66350873 | 66350953 | 66350873 | ID=cds.MS.gene060424.t1;Parent=MS.gene060424.t1 |
| chr7.1 | exon | 66350628 | 66350705 | 66350628 | ID=MS.gene060424.t1.exon2;Parent=MS.gene060424.t1 |
| chr7.1 | CDS | 66350628 | 66350705 | 66350628 | ID=cds.MS.gene060424.t1;Parent=MS.gene060424.t1 |
| chr7.1 | exon | 66350459 | 66350538 | 66350459 | ID=MS.gene060424.t1.exon3;Parent=MS.gene060424.t1 |
| chr7.1 | CDS | 66350459 | 66350538 | 66350459 | ID=cds.MS.gene060424.t1;Parent=MS.gene060424.t1 |
| chr7.1 | exon | 66350371 | 66350436 | 66350371 | ID=MS.gene060424.t1.exon4;Parent=MS.gene060424.t1 |
| chr7.1 | CDS | 66350371 | 66350436 | 66350371 | ID=cds.MS.gene060424.t1;Parent=MS.gene060424.t1 |
| chr7.1 | exon | 66350213 | 66350264 | 66350213 | ID=MS.gene060424.t1.exon5;Parent=MS.gene060424.t1 |
| chr7.1 | CDS | 66350213 | 66350264 | 66350213 | ID=cds.MS.gene060424.t1;Parent=MS.gene060424.t1 |
| chr7.1 | exon | 66349792 | 66349896 | 66349792 | ID=MS.gene060424.t1.exon6;Parent=MS.gene060424.t1 |
| chr7.1 | CDS | 66349792 | 66349896 | 66349792 | ID=cds.MS.gene060424.t1;Parent=MS.gene060424.t1 |
| Gene Sequence |
| Protein sequence |
