AlfalfaGEDB | MS.gene86061

M. sativa cultivar XinJiangDaYe / MS.gene86061

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86061.t1	KEH25024.1	41.2	148	59	2	75	194	54	201	4.90E-19	104.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86061.t1	A0A072U5J3	41.2	148	59	2	75	194	54	201	3.5e-19	104.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04915	MS.gene86061	0.812392	4.34E-51	-1.69E-46
MS.gene04917	MS.gene86061	0.813045	3.12E-51	-1.69E-46
MS.gene04998	MS.gene86061	0.818075	2.37E-52	-1.69E-46
MS.gene050114	MS.gene86061	0.809547	1.79E-50	-1.69E-46
MS.gene050317	MS.gene86061	0.814861	1.24E-51	-1.69E-46
MS.gene050320	MS.gene86061	0.807402	5.15E-50	-1.69E-46
MS.gene050414	MS.gene86061	0.801857	7.39E-49	-1.69E-46
MS.gene050654	MS.gene86061	0.816938	4.27E-52	-1.69E-46
MS.gene050752	MS.gene86061	0.834256	3.33E-56	-1.69E-46
MS.gene050838	MS.gene86061	0.868854	4.79E-66	-1.69E-46
MS.gene050945	MS.gene86061	0.809265	2.06E-50	-1.69E-46
MS.gene050955	MS.gene86061	0.815303	9.91E-52	-1.69E-46
MS.gene051003	MS.gene86061	0.839586	1.45E-57	-1.69E-46
MS.gene051043	MS.gene86061	0.805723	1.16E-49	-1.69E-46
MS.gene051233	MS.gene86061	0.87243	3.20E-67	-1.69E-46
MS.gene051234	MS.gene86061	0.828409	9.14E-55	-1.69E-46
MS.gene051343	MS.gene86061	0.835344	1.77E-56	-1.69E-46
MS.gene051676	MS.gene86061	0.800316	1.53E-48	-1.69E-46
MS.gene051748	MS.gene86061	0.813882	2.04E-51	-1.69E-46
MS.gene051902	MS.gene86061	0.863265	2.82E-64	-1.69E-46
MS.gene051903	MS.gene86061	0.818846	1.58E-52	-1.69E-46
MS.gene052242	MS.gene86061	-0.808159	3.55E-50	-1.69E-46
MS.gene052296	MS.gene86061	0.844013	9.83E-59	-1.69E-46
MS.gene052335	MS.gene86061	0.831603	1.52E-55	-1.69E-46
MS.gene052342	MS.gene86061	0.841728	3.98E-58	-1.69E-46
MS.gene052434	MS.gene86061	0.814558	1.45E-51	-1.69E-46
MS.gene052479	MS.gene86061	0.821305	4.33E-53	-1.69E-46
MS.gene052818	MS.gene86061	-0.869557	2.83E-66	-1.69E-46
MS.gene052947	MS.gene86061	0.823503	1.34E-53	-1.69E-46
MS.gene053058	MS.gene86061	0.847617	1.03E-59	-1.69E-46
MS.gene053298	MS.gene86061	0.826473	2.66E-54	-1.69E-46
MS.gene053564	MS.gene86061	0.828482	8.78E-55	-1.69E-46
MS.gene053677	MS.gene86061	0.818523	1.87E-52	-1.69E-46
MS.gene053786	MS.gene86061	0.825112	5.60E-54	-1.69E-46
MS.gene053879	MS.gene86061	0.802405	5.70E-49	-1.69E-46
MS.gene054403	MS.gene86061	0.836165	1.10E-56	-1.69E-46
MS.gene05469	MS.gene86061	0.800203	1.61E-48	-1.69E-46
MS.gene055174	MS.gene86061	0.805868	1.08E-49	-1.69E-46
MS.gene055424	MS.gene86061	0.813783	2.15E-51	-1.69E-46
MS.gene056181	MS.gene86061	0.807037	6.15E-50	-1.69E-46
MS.gene056580	MS.gene86061	0.806061	9.87E-50	-1.69E-46
MS.gene056752	MS.gene86061	0.849911	2.39E-60	-1.69E-46
MS.gene056764	MS.gene86061	0.806654	7.41E-50	-1.69E-46
MS.gene056830	MS.gene86061	-0.825245	5.21E-54	-1.69E-46
MS.gene057057	MS.gene86061	0.815084	1.11E-51	-1.69E-46
MS.gene057094	MS.gene86061	0.816544	5.24E-52	-1.69E-46
MS.gene057234	MS.gene86061	0.812983	3.22E-51	-1.69E-46
MS.gene057452	MS.gene86061	-0.83575	1.40E-56	-1.69E-46
MS.gene057576	MS.gene86061	0.812259	4.64E-51	-1.69E-46
MS.gene057705	MS.gene86061	0.825276	5.12E-54	-1.69E-46
MS.gene05781	MS.gene86061	0.832436	9.46E-56	-1.69E-46
MS.gene057890	MS.gene86061	-0.83528	1.84E-56	-1.69E-46
MS.gene057999	MS.gene86061	0.856821	2.48E-62	-1.69E-46
MS.gene058054	MS.gene86061	0.802616	5.16E-49	-1.69E-46
MS.gene058074	MS.gene86061	0.851024	1.16E-60	-1.69E-46
MS.gene058238	MS.gene86061	0.80074	1.25E-48	-1.69E-46
MS.gene058261	MS.gene86061	0.857498	1.57E-62	-1.69E-46
MS.gene058654	MS.gene86061	0.803009	4.28E-49	-1.69E-46
MS.gene059146	MS.gene86061	0.833465	5.25E-56	-1.69E-46
MS.gene059171	MS.gene86061	0.805013	1.64E-49	-1.69E-46
MS.gene059872	MS.gene86061	-0.842663	2.25E-58	-1.69E-46
MS.gene06011	MS.gene86061	0.838957	2.11E-57	-1.69E-46
MS.gene060259	MS.gene86061	0.803837	2.88E-49	-1.69E-46
MS.gene060424	MS.gene86061	0.82339	1.42E-53	-1.69E-46
MS.gene060563	MS.gene86061	0.807795	4.24E-50	-1.69E-46
MS.gene060763	MS.gene86061	0.824013	1.02E-53	-1.69E-46
MS.gene061100	MS.gene86061	0.806201	9.23E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86061.t1	MTR_6g013220	40.816	147	59	2	76	194	55	201	1.04e-26	101
MS.gene86061.t1	MTR_6g048070	46.392	97	36	2	2	82	1	97	3.55e-19	79.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 35 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCATAACACTCGACTGTTAA+AGG	0.149363	6.3:+8428280	None:intergenic
TCGCCATTGGAACGTGTTTC+CGG	0.199168	6.3:+8428658	None:intergenic
AATTTACCCATCATCATAAA+AGG	0.231721	6.3:+8428427	None:intergenic
TTAAAGGATGGTGGAAGGAG+GGG	0.305387	6.3:-8428705	MS.gene86061:CDS
CTCACTGAGGAAAAGGTTAA+AGG	0.307407	6.3:-8428721	MS.gene86061:CDS
AACAAATAAGAGATGGATCT+TGG	0.327273	6.3:-8428575	MS.gene86061:CDS
GTCAAGAGAAAGCAAATTTA+CGG	0.332482	6.3:-8428486	MS.gene86061:CDS
TCAATCAATCCAACCATAAC+TGG	0.335455	6.3:+8428760	None:intergenic
TCATATGTTTAATCCAGTTA+TGG	0.410645	6.3:-8428773	MS.gene86061:CDS
TAAAGGATGGTGGAAGGAGG+GGG	0.411581	6.3:-8428704	MS.gene86061:CDS
GCTTTCTCTTGACTACAATC+TGG	0.415041	6.3:+8428496	None:intergenic
GGTTAAAGGATGGTGGAAGG+AGG	0.417552	6.3:-8428707	MS.gene86061:CDS
AGTAGGCTGGATAGATGTTC+CGG	0.432114	6.3:-8428677	MS.gene86061:CDS
TTCAAGGCTCACTGAGGAAA+AGG	0.447645	6.3:-8428728	MS.gene86061:CDS
GTTAAAGGATGGTGGAAGGA+GGG	0.464998	6.3:-8428706	MS.gene86061:CDS
ATGATGGGTAAATTTAAAGA+TGG	0.496580	6.3:-8428418	MS.gene86061:CDS
AAGAGATGGATCTTGGTTGA+AGG	0.508170	6.3:-8428568	MS.gene86061:CDS
GATGTGAAATTGTCTTAATG+AGG	0.509961	6.3:+8428449	None:intergenic
ATGTTTAATCCAGTTATGGT+TGG	0.517438	6.3:-8428769	MS.gene86061:CDS
TGATTGACACAAAACATTCA+AGG	0.536208	6.3:-8428744	MS.gene86061:CDS
CTTCAGAAACGTATTGATGT+GGG	0.540400	6.3:-8428538	MS.gene86061:CDS
GAAGTTTAACAAATAAGAGA+TGG	0.544075	6.3:-8428582	MS.gene86061:CDS
GCTTCAGAAACGTATTGATG+TGG	0.565440	6.3:-8428539	MS.gene86061:CDS
TGGCGAGAAAAGTGAGGGAA+TGG	0.574001	6.3:-8428641	MS.gene86061:CDS
TGAATGCTCTCAGATTGTCC+TGG	0.580896	6.3:+8428805	None:intergenic
GTGAGGGAATGGTATTCGCA+TGG	0.581460	6.3:-8428630	MS.gene86061:CDS
AGGAAAAGGTTAAAGGATGG+TGG	0.583886	6.3:-8428714	MS.gene86061:CDS
ACGATGTCGGGATTAGAGAC+AGG	0.585282	6.3:-8428319	MS.gene86061:CDS
AAAGGTTAAAGGATGGTGGA+AGG	0.586600	6.3:-8428710	MS.gene86061:CDS
GTTCCGGAAACACGTTCCAA+TGG	0.594759	6.3:-8428661	MS.gene86061:CDS
TCCAATGGCGAGAAAAGTGA+GGG	0.612722	6.3:-8428646	MS.gene86061:CDS
TTCCAATGGCGAGAAAAGTG+AGG	0.630676	6.3:-8428647	MS.gene86061:CDS
GTCACAGGAATTAATCAACC+AGG	0.651057	6.3:-8428823	MS.gene86061:CDS
CTGAGGAAAAGGTTAAAGGA+TGG	0.658281	6.3:-8428717	MS.gene86061:CDS
AAAACATTCAAGGCTCACTG+AGG	0.736310	6.3:-8428734	MS.gene86061:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATGAACAAAATGTCAATTTT+AGG	-	chr6.3:8428727-8428746	MS.gene86061:CDS	20.0%
!!!	TGAACAAAATGTCAATTTTA+GGG	-	chr6.3:8428728-8428747	MS.gene86061:CDS	20.0%
!!!	TTAAAAAGCTCTGAAAATTT+AGG	+	chr6.3:8428765-8428784	None:intergenic	20.0%
!	AATTTACCCATCATCATAAA+AGG	+	chr6.3:8428687-8428706	None:intergenic	25.0%
!	GAAGTTTAACAAATAAGAGA+TGG	-	chr6.3:8428529-8428548	MS.gene86061:CDS	25.0%
!	TCATATGTTTAATCCAGTTA+TGG	-	chr6.3:8428338-8428357	MS.gene86061:CDS	25.0%
!!	AAATTGACATTTTGTTCATC+TGG	+	chr6.3:8428726-8428745	None:intergenic	25.0%
!!!	ATGATGGGTAAATTTAAAGA+TGG	-	chr6.3:8428693-8428712	MS.gene86061:CDS	25.0%
	AACAAATAAGAGATGGATCT+TGG	-	chr6.3:8428536-8428555	MS.gene86061:CDS	30.0%
	ATGTTTAATCCAGTTATGGT+TGG	-	chr6.3:8428342-8428361	MS.gene86061:CDS	30.0%
	GATGTGAAATTGTCTTAATG+AGG	+	chr6.3:8428665-8428684	None:intergenic	30.0%
	GTCAAGAGAAAGCAAATTTA+CGG	-	chr6.3:8428625-8428644	MS.gene86061:CDS	30.0%
	TGATTGACACAAAACATTCA+AGG	-	chr6.3:8428367-8428386	MS.gene86061:CDS	30.0%
!	TATTGATGTGGGTTTTCTTT+TGG	-	chr6.3:8428584-8428603	MS.gene86061:CDS	30.0%
!	TCACATCCTTTTATGATGAT+GGG	-	chr6.3:8428678-8428697	MS.gene86061:CDS	30.0%
!	TTCACATCCTTTTATGATGA+TGG	-	chr6.3:8428677-8428696	MS.gene86061:CDS	30.0%
!!!	TTTTAAGTTTTCGACGATGT+CGG	-	chr6.3:8428779-8428798	MS.gene86061:CDS	30.0%
	TCAATCAATCCAACCATAAC+TGG	+	chr6.3:8428354-8428373	None:intergenic	35.0%
	TCATAACACTCGACTGTTAA+AGG	+	chr6.3:8428834-8428853	None:intergenic	35.0%
!	CTTCAGAAACGTATTGATGT+GGG	-	chr6.3:8428573-8428592	MS.gene86061:CDS	35.0%
!	TTGACATTTTGTTCATCTGG+TGG	+	chr6.3:8428723-8428742	None:intergenic	35.0%
!!	TTTAAGTTTTCGACGATGTC+GGG	-	chr6.3:8428780-8428799	MS.gene86061:CDS	35.0%
!!!	ATTTTAGGTTGGAATGTCAC+AGG	-	chr6.3:8428273-8428292	MS.gene86061:CDS	35.0%
!!!	GTCAATTTTAGGGTTTTTGC+AGG	-	chr6.3:8428738-8428757	MS.gene86061:CDS	35.0%
	AAAACATTCAAGGCTCACTG+AGG	-	chr6.3:8428377-8428396	MS.gene86061:CDS	40.0%
	AAGAGATGGATCTTGGTTGA+AGG	-	chr6.3:8428543-8428562	MS.gene86061:CDS	40.0%
	AGGAAAAGGTTAAAGGATGG+TGG	-	chr6.3:8428397-8428416	MS.gene86061:CDS	40.0%
	CTCACTGAGGAAAAGGTTAA+AGG	-	chr6.3:8428390-8428409	MS.gene86061:CDS	40.0%
	CTGAGGAAAAGGTTAAAGGA+TGG	-	chr6.3:8428394-8428413	MS.gene86061:CDS	40.0%
	GCTTTCTCTTGACTACAATC+TGG	+	chr6.3:8428618-8428637	None:intergenic	40.0%
	GTCACAGGAATTAATCAACC+AGG	-	chr6.3:8428288-8428307	MS.gene86061:CDS	40.0%
!	GCTTCAGAAACGTATTGATG+TGG	-	chr6.3:8428572-8428591	MS.gene86061:CDS	40.0%
!!	AAAGGTTAAAGGATGGTGGA+AGG	-	chr6.3:8428401-8428420	MS.gene86061:CDS	40.0%
	AGTAGGCTGGATAGATGTTC+CGG	-	chr6.3:8428434-8428453	MS.gene86061:CDS	45.0%
	TCCAATGGCGAGAAAAGTGA+GGG	-	chr6.3:8428465-8428484	MS.gene86061:CDS	45.0%
	TGAATGCTCTCAGATTGTCC+TGG	+	chr6.3:8428309-8428328	None:intergenic	45.0%
	TTCAAGGCTCACTGAGGAAA+AGG	-	chr6.3:8428383-8428402	MS.gene86061:CDS	45.0%
	TTCCAATGGCGAGAAAAGTG+AGG	-	chr6.3:8428464-8428483	MS.gene86061:CDS	45.0%
!!	GTTAAAGGATGGTGGAAGGA+GGG	-	chr6.3:8428405-8428424	MS.gene86061:CDS	45.0%
!!	TTAAAGGATGGTGGAAGGAG+GGG	-	chr6.3:8428406-8428425	MS.gene86061:CDS	45.0%
	ACGATGTCGGGATTAGAGAC+AGG	-	chr6.3:8428792-8428811	MS.gene86061:CDS	50.0%
	GTGAGGGAATGGTATTCGCA+TGG	-	chr6.3:8428481-8428500	MS.gene86061:CDS	50.0%
	GTTCCGGAAACACGTTCCAA+TGG	-	chr6.3:8428450-8428469	MS.gene86061:CDS	50.0%
	TCGCCATTGGAACGTGTTTC+CGG	+	chr6.3:8428456-8428475	None:intergenic	50.0%
	TGGCGAGAAAAGTGAGGGAA+TGG	-	chr6.3:8428470-8428489	MS.gene86061:CDS	50.0%
!!	GGTTAAAGGATGGTGGAAGG+AGG	-	chr6.3:8428404-8428423	MS.gene86061:CDS	50.0%
!!	TAAAGGATGGTGGAAGGAGG+GGG	-	chr6.3:8428407-8428426	MS.gene86061:CDS	50.0%
!!	TCCCTCACTTTTCTCGCCAT+TGG	+	chr6.3:8428469-8428488	None:intergenic	50.0%
	TGGAAGGAGGGGGACAAAGT+AGG	-	chr6.3:8428417-8428436	MS.gene86061:CDS	55.0%
	AGGAGGGGGACAAAGTAGGC+TGG	-	chr6.3:8428421-8428440	MS.gene86061:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	8428264	8428869	8428264	ID=MS.gene86061
chr6.3	mRNA	8428264	8428869	8428264	ID=MS.gene86061.t1;Parent=MS.gene86061
chr6.3	exon	8428264	8428869	8428264	ID=MS.gene86061.t1.exon1;Parent=MS.gene86061.t1
chr6.3	CDS	8428264	8428869	8428264	ID=cds.MS.gene86061.t1;Parent=MS.gene86061.t1

Gene Sequence

>MS.gene86061

ATGATGAATATTTTAGGTTGGAATGTCACAGGAATTAATCAACCAGGACAATCTGAGAGCATTCATCAGCTTGTTCATATGTTTAATCCAGTTATGGTTGGATTGATTGACACAAAACATTCAAGGCTCACTGAGGAAAAGGTTAAAGGATGGTGGAAGGAGGGGGACAAAGTAGGCTGGATAGATGTTCCGGAAACACGTTCCAATGGCGAGAAAAGTGAGGGAATGGTATTCGCATGGAATGAACGTTGTTTTATGAAATCAAGAAGTTTAACAAATAAGAGATGGATCTTGGTTGAAGGAAAGCTGCTTCAGAAACGTATTGATGTGGGTTTTCTTTTGGTTTTTGAACCAGATTGTAGTCAAGAGAAAGCAAATTTACGGAATGATTTGACGAACCTCATTAAGACAATTTCACATCCTTTTATGATGATGGGTAAATTTAAAGATGGTTCGCCACCAGATGAACAAAATGTCAATTTTAGGGTTTTTGCAGGTCCTAAATTTTCAGAGCTTTTTAAGTTTTCGACGATGTCGGGATTAGAGACAGGTCTCTCAGATCACTGTCCTTTAACAGTCGAGTGTTATGAAAAAGATAATATTTGA

Protein sequence

>MS.gene86061.t1

MMNILGWNVTGINQPGQSESIHQLVHMFNPVMVGLIDTKHSRLTEEKVKGWWKEGDKVGWIDVPETRSNGEKSEGMVFAWNERCFMKSRSLTNKRWILVEGKLLQKRIDVGFLLVFEPDCSQEKANLRNDLTNLIKTISHPFMMMGKFKDGSPPDEQNVNFRVFAGPKFSELFKFSTMSGLETGLSDHCPLTVECYEKDNI