Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41088.t1 | XP_013467905.1 | 97.6 | 868 | 21 | 0 | 1 | 868 | 1 | 868 | 0.00E+00 | 1572.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41088.t1 | F4IYF8 | 54.8 | 891 | 366 | 13 | 2 | 866 | 4 | 883 | 3.1e-252 | 872.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41088.t1 | A0A072VJI1 | 97.6 | 868 | 21 | 0 | 1 | 868 | 1 | 868 | 0.0e+00 | 1572.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene41088 | 0.805468 | 1.32E-49 | -1.69E-46 |
| MS.gene049208 | MS.gene41088 | 0.824321 | 8.60E-54 | -1.69E-46 |
| MS.gene049283 | MS.gene41088 | 0.815969 | 7.04E-52 | -1.69E-46 |
| MS.gene049368 | MS.gene41088 | 0.801287 | 9.67E-49 | -1.69E-46 |
| MS.gene049411 | MS.gene41088 | 0.819713 | 1.01E-52 | -1.69E-46 |
| MS.gene049807 | MS.gene41088 | 0.805042 | 1.62E-49 | -1.69E-46 |
| MS.gene049887 | MS.gene41088 | 0.807429 | 5.08E-50 | -1.69E-46 |
| MS.gene050084 | MS.gene41088 | 0.815546 | 8.75E-52 | -1.69E-46 |
| MS.gene050085 | MS.gene41088 | 0.844694 | 6.45E-59 | -1.69E-46 |
| MS.gene050114 | MS.gene41088 | 0.811638 | 6.33E-51 | -1.69E-46 |
| MS.gene050359 | MS.gene41088 | 0.82031 | 7.34E-53 | -1.69E-46 |
| MS.gene050414 | MS.gene41088 | 0.804971 | 1.67E-49 | -1.69E-46 |
| MS.gene050551 | MS.gene41088 | 0.841447 | 4.73E-58 | -1.69E-46 |
| MS.gene050556 | MS.gene41088 | 0.821005 | 5.08E-53 | -1.69E-46 |
| MS.gene050646 | MS.gene41088 | 0.82666 | 2.40E-54 | -1.69E-46 |
| MS.gene050654 | MS.gene41088 | 0.809521 | 1.82E-50 | -1.69E-46 |
| MS.gene050689 | MS.gene41088 | 0.852167 | 5.51E-61 | -1.69E-46 |
| MS.gene050752 | MS.gene41088 | 0.811282 | 7.57E-51 | -1.69E-46 |
| MS.gene050838 | MS.gene41088 | 0.908213 | 2.18E-81 | -1.69E-46 |
| MS.gene050945 | MS.gene41088 | 0.860491 | 1.99E-63 | -1.69E-46 |
| MS.gene051003 | MS.gene41088 | 0.858392 | 8.50E-63 | -1.69E-46 |
| MS.gene051043 | MS.gene41088 | 0.81283 | 3.48E-51 | -1.69E-46 |
| MS.gene051102 | MS.gene41088 | 0.825268 | 5.14E-54 | -1.69E-46 |
| MS.gene051233 | MS.gene41088 | 0.851517 | 8.43E-61 | -1.69E-46 |
| MS.gene051234 | MS.gene41088 | 0.88311 | 5.92E-71 | -1.69E-46 |
| MS.gene051343 | MS.gene41088 | 0.887539 | 1.31E-72 | -1.69E-46 |
| MS.gene051381 | MS.gene41088 | 0.86133 | 1.11E-63 | -1.69E-46 |
| MS.gene051382 | MS.gene41088 | 0.819514 | 1.12E-52 | -1.69E-46 |
| MS.gene051450 | MS.gene41088 | 0.822762 | 1.99E-53 | -1.69E-46 |
| MS.gene051642 | MS.gene41088 | 0.823114 | 1.65E-53 | -1.69E-46 |
| MS.gene051676 | MS.gene41088 | 0.840614 | 7.82E-58 | -1.69E-46 |
| MS.gene051700 | MS.gene41088 | 0.803717 | 3.05E-49 | -1.69E-46 |
| MS.gene051748 | MS.gene41088 | 0.817321 | 3.50E-52 | -1.69E-46 |
| MS.gene051850 | MS.gene41088 | 0.815672 | 8.20E-52 | -1.69E-46 |
| MS.gene051885 | MS.gene41088 | 0.814294 | 1.66E-51 | -1.69E-46 |
| MS.gene05188 | MS.gene41088 | 0.807847 | 4.14E-50 | -1.69E-46 |
| MS.gene051902 | MS.gene41088 | 0.86231 | 5.55E-64 | -1.69E-46 |
| MS.gene051975 | MS.gene41088 | 0.844859 | 5.82E-59 | -1.69E-46 |
| MS.gene052072 | MS.gene41088 | 0.851132 | 1.08E-60 | -1.69E-46 |
| MS.gene052242 | MS.gene41088 | -0.83439 | 3.08E-56 | -1.69E-46 |
| MS.gene052307 | MS.gene41088 | 0.859144 | 5.07E-63 | -1.69E-46 |
| MS.gene052335 | MS.gene41088 | 0.846069 | 2.74E-59 | -1.69E-46 |
| MS.gene052342 | MS.gene41088 | 0.828664 | 7.93E-55 | -1.69E-46 |
| MS.gene052397 | MS.gene41088 | 0.807577 | 4.72E-50 | -1.69E-46 |
| MS.gene052434 | MS.gene41088 | 0.824263 | 8.87E-54 | -1.69E-46 |
| MS.gene052609 | MS.gene41088 | 0.863883 | 1.81E-64 | -1.69E-46 |
| MS.gene052932 | MS.gene41088 | 0.837226 | 5.89E-57 | -1.69E-46 |
| MS.gene052947 | MS.gene41088 | 0.832692 | 8.17E-56 | -1.69E-46 |
| MS.gene053058 | MS.gene41088 | 0.894102 | 3.38E-75 | -1.69E-46 |
| MS.gene053079 | MS.gene41088 | 0.831036 | 2.10E-55 | -1.69E-46 |
| MS.gene053122 | MS.gene41088 | 0.825305 | 5.04E-54 | -1.69E-46 |
| MS.gene053143 | MS.gene41088 | 0.862695 | 4.22E-64 | -1.69E-46 |
| MS.gene053167 | MS.gene41088 | 0.809012 | 2.33E-50 | -1.69E-46 |
| MS.gene053564 | MS.gene41088 | 0.829486 | 5.01E-55 | -1.69E-46 |
| MS.gene053581 | MS.gene41088 | 0.802747 | 4.84E-49 | -1.69E-46 |
| MS.gene05364 | MS.gene41088 | 0.8242 | 9.18E-54 | -1.69E-46 |
| MS.gene053786 | MS.gene41088 | 0.822554 | 2.23E-53 | -1.69E-46 |
| MS.gene053952 | MS.gene41088 | 0.830735 | 2.48E-55 | -1.69E-46 |
| MS.gene054004 | MS.gene41088 | 0.80418 | 2.45E-49 | -1.69E-46 |
| MS.gene054403 | MS.gene41088 | 0.810268 | 1.25E-50 | -1.69E-46 |
| MS.gene054413 | MS.gene41088 | 0.845748 | 3.35E-59 | -1.69E-46 |
| MS.gene054824 | MS.gene41088 | 0.815831 | 7.56E-52 | -1.69E-46 |
| MS.gene055311 | MS.gene41088 | 0.847933 | 8.45E-60 | -1.69E-46 |
| MS.gene055313 | MS.gene41088 | 0.844065 | 9.52E-59 | -1.69E-46 |
| MS.gene055314 | MS.gene41088 | 0.851717 | 7.40E-61 | -1.69E-46 |
| MS.gene055424 | MS.gene41088 | 0.819706 | 1.01E-52 | -1.69E-46 |
| MS.gene055469 | MS.gene41088 | 0.820738 | 5.85E-53 | -1.69E-46 |
| MS.gene055513 | MS.gene41088 | 0.883504 | 4.24E-71 | -1.69E-46 |
| MS.gene055975 | MS.gene41088 | 0.807524 | 4.85E-50 | -1.69E-46 |
| MS.gene056043 | MS.gene41088 | -0.804418 | 2.18E-49 | -1.69E-46 |
| MS.gene056045 | MS.gene41088 | 0.850922 | 1.24E-60 | -1.69E-46 |
| MS.gene056097 | MS.gene41088 | 0.842779 | 2.10E-58 | -1.69E-46 |
| MS.gene056181 | MS.gene41088 | 0.87021 | 1.73E-66 | -1.69E-46 |
| MS.gene056308 | MS.gene41088 | 0.826304 | 2.92E-54 | -1.69E-46 |
| MS.gene056390 | MS.gene41088 | 0.817865 | 2.64E-52 | -1.69E-46 |
| MS.gene056403 | MS.gene41088 | 0.855535 | 5.91E-62 | -1.69E-46 |
| MS.gene056507 | MS.gene41088 | 0.800287 | 1.55E-48 | -1.69E-46 |
| MS.gene056578 | MS.gene41088 | 0.851599 | 7.99E-61 | -1.69E-46 |
| MS.gene056605 | MS.gene41088 | 0.844295 | 8.26E-59 | -1.69E-46 |
| MS.gene056787 | MS.gene41088 | 0.81226 | 4.64E-51 | -1.69E-46 |
| MS.gene05689 | MS.gene41088 | 0.83344 | 5.33E-56 | -1.69E-46 |
| MS.gene056986 | MS.gene41088 | 0.815313 | 9.85E-52 | -1.69E-46 |
| MS.gene057094 | MS.gene41088 | 0.838472 | 2.82E-57 | -1.69E-46 |
| MS.gene057102 | MS.gene41088 | 0.832764 | 7.85E-56 | -1.69E-46 |
| MS.gene057705 | MS.gene41088 | 0.811924 | 5.49E-51 | -1.69E-46 |
| MS.gene05781 | MS.gene41088 | 0.85202 | 6.07E-61 | -1.69E-46 |
| MS.gene057890 | MS.gene41088 | -0.822296 | 2.56E-53 | -1.69E-46 |
| MS.gene057999 | MS.gene41088 | 0.808296 | 3.32E-50 | -1.69E-46 |
| MS.gene058054 | MS.gene41088 | 0.821383 | 4.16E-53 | -1.69E-46 |
| MS.gene058055 | MS.gene41088 | 0.801719 | 7.89E-49 | -1.69E-46 |
| MS.gene058074 | MS.gene41088 | 0.818632 | 1.77E-52 | -1.69E-46 |
| MS.gene058261 | MS.gene41088 | 0.820706 | 5.95E-53 | -1.69E-46 |
| MS.gene058292 | MS.gene41088 | 0.807904 | 4.02E-50 | -1.69E-46 |
| MS.gene058638 | MS.gene41088 | 0.834169 | 3.50E-56 | -1.69E-46 |
| MS.gene058639 | MS.gene41088 | 0.840596 | 7.91E-58 | -1.69E-46 |
| MS.gene058758 | MS.gene41088 | 0.808143 | 3.58E-50 | -1.69E-46 |
| MS.gene05880 | MS.gene41088 | 0.812638 | 3.83E-51 | -1.69E-46 |
| MS.gene058852 | MS.gene41088 | 0.818511 | 1.89E-52 | -1.69E-46 |
| MS.gene059007 | MS.gene41088 | 0.822019 | 2.96E-53 | -1.69E-46 |
| MS.gene059146 | MS.gene41088 | 0.874294 | 7.56E-68 | -1.69E-46 |
| MS.gene059170 | MS.gene41088 | 0.807295 | 5.42E-50 | -1.69E-46 |
| MS.gene059171 | MS.gene41088 | 0.858305 | 9.03E-63 | -1.69E-46 |
| MS.gene059227 | MS.gene41088 | 0.827643 | 1.40E-54 | -1.69E-46 |
| MS.gene059778 | MS.gene41088 | 0.823526 | 1.32E-53 | -1.69E-46 |
| MS.gene05978 | MS.gene41088 | 0.835068 | 2.08E-56 | -1.69E-46 |
| MS.gene05985 | MS.gene41088 | -0.806335 | 8.65E-50 | -1.69E-46 |
| MS.gene059965 | MS.gene41088 | 0.817565 | 3.09E-52 | -1.69E-46 |
| MS.gene06001 | MS.gene41088 | 0.810486 | 1.13E-50 | -1.69E-46 |
| MS.gene06011 | MS.gene41088 | 0.812374 | 4.38E-51 | -1.69E-46 |
| MS.gene060343 | MS.gene41088 | -0.820983 | 5.14E-53 | -1.69E-46 |
| MS.gene060365 | MS.gene41088 | 0.815348 | 9.68E-52 | -1.69E-46 |
| MS.gene060424 | MS.gene41088 | 0.800309 | 1.53E-48 | -1.69E-46 |
| MS.gene060563 | MS.gene41088 | 0.804269 | 2.34E-49 | -1.69E-46 |
| MS.gene060763 | MS.gene41088 | 0.848214 | 7.07E-60 | -1.69E-46 |
| MS.gene060933 | MS.gene41088 | 0.840408 | 8.85E-58 | -1.69E-46 |
| MS.gene060937 | MS.gene41088 | 0.810015 | 1.42E-50 | -1.69E-46 |
| MS.gene061006 | MS.gene41088 | 0.868978 | 4.37E-66 | -1.69E-46 |
| MS.gene061100 | MS.gene41088 | 0.874561 | 6.14E-68 | -1.69E-46 |
| MS.gene061160 | MS.gene41088 | 0.80012 | 1.67E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene43889 | MS.gene41088 | PPI |
| MS.gene35246 | MS.gene41088 | PPI |
| MS.gene45075 | MS.gene41088 | PPI |
| MS.gene82886 | MS.gene41088 | PPI |
| MS.gene026998 | MS.gene41088 | PPI |
| MS.gene027585 | MS.gene41088 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41088.t1 | MTR_1g057860 | 97.581 | 868 | 21 | 0 | 1 | 868 | 1 | 868 | 0.0 | 1725 |
| MS.gene41088.t1 | MTR_1g022010 | 69.756 | 205 | 32 | 3 | 582 | 780 | 49 | 229 | 3.04e-85 | 275 |
| MS.gene41088.t1 | MTR_4g129940 | 78.472 | 144 | 29 | 1 | 428 | 569 | 40 | 183 | 4.15e-72 | 235 |
| MS.gene41088.t1 | MTR_1g057880 | 78.873 | 142 | 9 | 1 | 626 | 767 | 1 | 121 | 1.89e-66 | 218 |
| MS.gene41088.t1 | MTR_5g091860 | 89.655 | 116 | 12 | 0 | 627 | 742 | 13 | 128 | 3.44e-63 | 210 |
| MS.gene41088.t1 | MTR_7g101940 | 28.438 | 429 | 280 | 11 | 434 | 853 | 87 | 497 | 7.31e-42 | 160 |
| MS.gene41088.t1 | MTR_7g012030 | 69.318 | 88 | 27 | 0 | 445 | 532 | 1 | 88 | 2.84e-33 | 127 |
| MS.gene41088.t1 | MTR_6g038890 | 62.500 | 88 | 28 | 2 | 445 | 531 | 1 | 84 | 1.53e-27 | 111 |
| MS.gene41088.t1 | MTR_5g091840 | 54.032 | 124 | 33 | 3 | 768 | 868 | 1 | 123 | 1.29e-26 | 105 |
| MS.gene41088.t1 | MTR_6g046330 | 67.105 | 76 | 25 | 0 | 457 | 532 | 3 | 78 | 1.47e-25 | 108 |
| MS.gene41088.t1 | MTR_4g067160 | 26.535 | 456 | 276 | 20 | 439 | 862 | 788 | 1216 | 2.51e-23 | 107 |
| MS.gene41088.t1 | MTR_1g088900 | 50.667 | 75 | 15 | 1 | 496 | 570 | 95 | 147 | 2.27e-13 | 71.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41088.t1 | AT3G02760 | 56.341 | 891 | 352 | 12 | 2 | 866 | 4 | 883 | 0.0 | 905 |
| MS.gene41088.t1 | AT3G02760 | 56.341 | 891 | 352 | 12 | 2 | 866 | 4 | 883 | 0.0 | 905 |
| MS.gene41088.t1 | AT3G46100 | 30.324 | 432 | 268 | 14 | 434 | 853 | 72 | 482 | 3.26e-45 | 170 |
| MS.gene41088.t1 | AT3G59410 | 25.814 | 430 | 261 | 16 | 447 | 840 | 796 | 1203 | 2.33e-28 | 123 |
| MS.gene41088.t1 | AT3G59410 | 25.814 | 430 | 261 | 16 | 447 | 840 | 796 | 1203 | 2.33e-28 | 123 |
| MS.gene41088.t1 | AT3G59410 | 25.814 | 430 | 261 | 16 | 447 | 840 | 796 | 1203 | 2.55e-28 | 123 |
| MS.gene41088.t1 | AT3G59410 | 25.814 | 430 | 261 | 16 | 447 | 840 | 797 | 1204 | 2.68e-28 | 123 |
| MS.gene41088.t1 | AT3G59410 | 25.814 | 430 | 261 | 16 | 447 | 840 | 820 | 1227 | 3.06e-28 | 123 |
| MS.gene41088.t1 | AT5G03406 | 32.620 | 187 | 119 | 4 | 434 | 614 | 72 | 257 | 1.18e-20 | 93.2 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 10674 | gene | 5235 | 14771 | 5235 | ID=MS.gene41088 |
| 10674 | mRNA | 5235 | 14771 | 5235 | ID=MS.gene41088.t1;Parent=MS.gene41088 |
| 10674 | exon | 13467 | 14771 | 13467 | ID=MS.gene41088.t1.exon1;Parent=MS.gene41088.t1 |
| 10674 | CDS | 13467 | 14771 | 13467 | ID=cds.MS.gene41088.t1;Parent=MS.gene41088.t1 |
| 10674 | exon | 12628 | 12807 | 12628 | ID=MS.gene41088.t1.exon2;Parent=MS.gene41088.t1 |
| 10674 | CDS | 12628 | 12807 | 12628 | ID=cds.MS.gene41088.t1;Parent=MS.gene41088.t1 |
| 10674 | exon | 12107 | 12364 | 12107 | ID=MS.gene41088.t1.exon3;Parent=MS.gene41088.t1 |
| 10674 | CDS | 12107 | 12364 | 12107 | ID=cds.MS.gene41088.t1;Parent=MS.gene41088.t1 |
| 10674 | exon | 11247 | 11381 | 11247 | ID=MS.gene41088.t1.exon4;Parent=MS.gene41088.t1 |
| 10674 | CDS | 11247 | 11381 | 11247 | ID=cds.MS.gene41088.t1;Parent=MS.gene41088.t1 |
| 10674 | exon | 10146 | 10427 | 10146 | ID=MS.gene41088.t1.exon5;Parent=MS.gene41088.t1 |
| 10674 | CDS | 10146 | 10427 | 10146 | ID=cds.MS.gene41088.t1;Parent=MS.gene41088.t1 |
| 10674 | exon | 5972 | 6112 | 5972 | ID=MS.gene41088.t1.exon6;Parent=MS.gene41088.t1 |
| 10674 | CDS | 5972 | 6112 | 5972 | ID=cds.MS.gene41088.t1;Parent=MS.gene41088.t1 |
| 10674 | exon | 5235 | 5540 | 5235 | ID=MS.gene41088.t1.exon7;Parent=MS.gene41088.t1 |
| 10674 | CDS | 5235 | 5540 | 5235 | ID=cds.MS.gene41088.t1;Parent=MS.gene41088.t1 |
| Gene Sequence |
| Protein sequence |
