AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069828


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069828.t1 MTR_2g083180 100.000 110 0 0 1 110 1 110 1.41e-79 229
MS.gene069828.t1 MTR_2g083180 92.727 110 8 0 1 110 1 110 2.18e-72 211
MS.gene069828.t1 MTR_2g083180 100.000 100 0 0 1 100 1 100 1.35e-71 209
MS.gene069828.t1 MTR_4g046733 88.000 100 11 1 1 100 1 99 1.17e-62 186
MS.gene069828.t1 MTR_2g083180 95.699 93 4 0 8 100 10 102 2.29e-62 186
MS.gene069828.t1 MTR_2g083180 100.000 83 0 0 1 83 1 83 1.69e-58 175
MS.gene069828.t1 MTR_3g051950 78.000 100 20 1 1 100 1 98 1.39e-51 158
MS.gene069828.t1 MTR_5g092820 73.196 97 24 1 4 100 2 96 2.78e-47 147
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069828.t1 AT4G27130 88.000 100 11 1 1 100 1 99 2.63e-62 185
MS.gene069828.t1 AT5G54760 87.000 100 12 1 1 100 1 99 1.29e-61 184
MS.gene069828.t1 AT5G54760 87.000 100 12 1 1 100 1 99 1.29e-61 184
MS.gene069828.t1 AT5G54760 87.000 100 12 1 1 100 1 99 1.29e-61 184
MS.gene069828.t1 AT5G54760 87.000 100 12 1 1 100 1 99 1.29e-61 184
MS.gene069828.t1 AT1G54290 86.000 100 13 1 1 100 1 99 7.15e-61 182
MS.gene069828.t1 AT5G54940 75.000 100 23 1 1 100 1 98 3.03e-50 155
MS.gene069828.t1 AT5G54940 75.000 100 23 1 1 100 1 98 3.03e-50 155
MS.gene069828.t1 AT5G54940 75.000 100 23 1 1 100 1 98 3.03e-50 155

Find 25 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGAATGACTTCACATCTT+TGG 0.277476 2.1:-17893301 MS.gene069828:CDS
AATGCTGAGGACGCCGGTGC+TGG 0.285317 2.1:-17893627 MS.gene069828:CDS
TTCGCTGCTGTATACGAATA+TGG 0.326573 2.1:+17893586 None:intergenic
ATTCATTACCTGGACTAAAA+AGG 0.327921 2.1:+17893317 None:intergenic
CCTGTCCTTGCTCTGGGTCC+TGG 0.375764 2.1:+17893457 None:intergenic
AGACATACCTGTCCTTGCTC+TGG 0.415595 2.1:+17893450 None:intergenic
TACAACAAAACTCCTTCTTA+AGG 0.416659 2.1:+17893493 None:intergenic
GACATACCTGTCCTTGCTCT+GGG 0.463686 2.1:+17893451 None:intergenic
TGAATTCTTTCTTCAATCCC+TGG 0.465760 2.1:+17893538 None:intergenic
GTGTAGGTTATTCAACTTCA+AGG 0.470369 2.1:-17893363 MS.gene069828:intron
TTGTAATGGTACAGTTGTCC+AGG 0.470439 2.1:-17893475 MS.gene069828:CDS
ATGCTGAGGACGCCGGTGCT+GGG 0.477491 2.1:-17893626 MS.gene069828:CDS
TCAGCATTTGCCTCAGCAAA+AGG 0.478903 2.1:+17893642 None:intergenic
ATTCGTATACAGCAGCGAAA+TGG 0.491023 2.1:-17893582 MS.gene069828:CDS
CGCCGGTGCTGGGTCATCAA+AGG 0.522725 2.1:-17893616 MS.gene069828:CDS
AAAAGCTTGACAACTGTCCA+GGG 0.558576 2.1:-17893555 MS.gene069828:CDS
ATGTGAAGTCATTCATTACC+TGG 0.562547 2.1:+17893307 None:intergenic
TTCAACTTCAAGGCGATCAG+AGG 0.577444 2.1:-17893353 MS.gene069828:CDS
GAGGCAAATGCTGAGGACGC+CGG 0.578099 2.1:-17893633 MS.gene069828:CDS
CTCCTTTGATGACCCAGCAC+CGG 0.583753 2.1:+17893614 None:intergenic
GATTCTGAAAGACCTTAAGA+AGG 0.584832 2.1:-17893505 MS.gene069828:CDS
CCAGGACCCAGAGCAAGGAC+AGG 0.587475 2.1:-17893457 MS.gene069828:intron
GCACAAATTCCTACTGCCTA+CGG 0.609588 2.1:-17894597 MS.gene069828:intron
GTTGTCCAGGACCCAGAGCA+AGG 0.630590 2.1:-17893462 MS.gene069828:CDS
TCCAACTTACCGTAGGCAGT+AGG 0.690363 2.1:+17894588 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTATAAGTAAAAAAAAATA+GGG + chr2.1:17893742-17893761 None:intergenic 10.0%
!!! TTTGAATTTTTATTATCTTT+AGG - chr2.1:17893506-17893525 MS.gene069828:CDS 10.0%
!! CGTTATAAGTAAAAAAAAAT+AGG + chr2.1:17893743-17893762 None:intergenic 15.0%
!!! CTTCAAATAACATTTTTTTA+TGG - chr2.1:17894520-17894539 MS.gene069828:intron 15.0%
!!! GTTTTTAATGTTTATACTTT+GGG - chr2.1:17893919-17893938 MS.gene069828:intron 15.0%
!!! TTTCTAAAATAGTTTTTTCT+GGG - chr2.1:17893872-17893891 MS.gene069828:intron 15.0%
!!! TTTTCTAAAATAGTTTTTTC+TGG - chr2.1:17893871-17893890 MS.gene069828:intron 15.0%
!!! AAATTGGATTTTTAAAGCTT+AGG - chr2.1:17894054-17894073 MS.gene069828:intron 20.0%
!!! ATAACATTTTTTTATGGTGT+AGG - chr2.1:17894526-17894545 MS.gene069828:intron 20.0%
!!! ATATTGCTTTTACTTTTACT+TGG - chr2.1:17893704-17893723 MS.gene069828:intron 20.0%
!!! GGTTTTTAATGTTTATACTT+TGG - chr2.1:17893918-17893937 MS.gene069828:intron 20.0%
!!! TTCTAAAATAGTTTTTTCTG+GGG - chr2.1:17893873-17893892 MS.gene069828:intron 20.0%
!!! TTGATCTGAAATAGTATTTT+GGG - chr2.1:17894015-17894034 MS.gene069828:intron 20.0%
!!! TTTGATCTGAAATAGTATTT+TGG - chr2.1:17894014-17894033 MS.gene069828:intron 20.0%
! AAAAAATTCTGTGTGATTCT+TGG + chr2.1:17893854-17893873 None:intergenic 25.0%
! AGATGAAGAATAGAAATAGA+TGG - chr2.1:17894145-17894164 MS.gene069828:intron 25.0%
! ATAGAAGAATTTCTCATTTC+TGG + chr2.1:17893613-17893632 None:intergenic 25.0%
! TAAAAGCAATATCAGTAAGA+AGG + chr2.1:17893696-17893715 None:intergenic 25.0%
! TCAAAGTTATGTACAATGAT+TGG + chr2.1:17893999-17894018 None:intergenic 25.0%
! TTAAGCATCTTGTTCAATAA+TGG + chr2.1:17893970-17893989 None:intergenic 25.0%
!! AAATGTTATTTGAAGCAACA+AGG + chr2.1:17894515-17894534 None:intergenic 25.0%
!! AATGTTATTTGAAGCAACAA+GGG + chr2.1:17894514-17894533 None:intergenic 25.0%
!!! TGATCTGAAATAGTATTTTG+GGG - chr2.1:17894016-17894035 MS.gene069828:intron 25.0%
AATGAATGACTTCACATCTT+TGG - chr2.1:17894604-17894623 MS.gene069828:CDS 30.0%
ATATGATATATGACTCCTGT+GGG - chr2.1:17894218-17894237 MS.gene069828:intron 30.0%
ATTCATTACCTGGACTAAAA+AGG + chr2.1:17894591-17894610 None:intergenic 30.0%
CAATTATACTGTGTACTGTT+GGG - chr2.1:17893435-17893454 MS.gene069828:intron 30.0%
GAATTTACTAGTCAATTGTC+AGG + chr2.1:17893547-17893566 None:intergenic 30.0%
TACAACAAAACTCCTTCTTA+AGG + chr2.1:17894415-17894434 None:intergenic 30.0%
TCAATTATACTGTGTACTGT+TGG - chr2.1:17893434-17893453 MS.gene069828:intron 30.0%
! AATTTTAGATCCTTTTGCTG+AGG - chr2.1:17894253-17894272 MS.gene069828:intron 30.0%
! ATGTTATTTGAAGCAACAAG+GGG + chr2.1:17894513-17894532 None:intergenic 30.0%
!!! AAGAAGGAGTTTTGTTGTAA+TGG - chr2.1:17894416-17894435 MS.gene069828:intron 30.0%
AGGATCTTAACGTTTGTGAA+GGG - chr2.1:17894074-17894093 MS.gene069828:intron 35.0%
ATGTGAAGTCATTCATTACC+TGG + chr2.1:17894601-17894620 None:intergenic 35.0%
GATATGATATATGACTCCTG+TGG - chr2.1:17894217-17894236 MS.gene069828:intron 35.0%
GTGTAGGTTATTCAACTTCA+AGG - chr2.1:17894542-17894561 MS.gene069828:intron 35.0%
TACATAATGAATGCTGTGTG+AGG + chr2.1:17894126-17894145 None:intergenic 35.0%
TAGGATCTTAACGTTTGTGA+AGG - chr2.1:17894073-17894092 MS.gene069828:intron 35.0%
TGAATTCTTTCTTCAATCCC+TGG + chr2.1:17894370-17894389 None:intergenic 35.0%
TTCATGATATGCAGTCATTC+TGG - chr2.1:17893391-17893410 MS.gene069828:intron 35.0%
! ATATGCAGTCATTCTGGAAA+GGG - chr2.1:17893397-17893416 MS.gene069828:intron 35.0%
! GATTCTGAAAGACCTTAAGA+AGG - chr2.1:17894400-17894419 MS.gene069828:intron 35.0%
! GCTTAACGTGGTTTAAGATT+GGG - chr2.1:17893897-17893916 MS.gene069828:intron 35.0%
! GTCTTGAGGTGGTTTAAAAT+TGG - chr2.1:17894038-17894057 MS.gene069828:intron 35.0%
! TGCTTAACGTGGTTTAAGAT+TGG - chr2.1:17893896-17893915 MS.gene069828:intron 35.0%
!! AATCTGAGACAGCATTTTCA+AGG + chr2.1:17893463-17893482 None:intergenic 35.0%
!! CATTTCTGGAGTTTAGTTGA+AGG + chr2.1:17893668-17893687 None:intergenic 35.0%
!!! AATAGTATTTTGGGGTCTTG+AGG - chr2.1:17894024-17894043 MS.gene069828:intron 35.0%
AAAAAGCTTGACAACTGTCC+AGG - chr2.1:17894349-17894368 MS.gene069828:intron 40.0%
AAAAGCTTGACAACTGTCCA+GGG - chr2.1:17894350-17894369 MS.gene069828:intron 40.0%
ATAGAGTTCCAACTTACCGT+AGG + chr2.1:17893327-17893346 None:intergenic 40.0%
ATTCGTATACAGCAGCGAAA+TGG - chr2.1:17894323-17894342 MS.gene069828:intron 40.0%
GTAAGAAGGCAACTCATTTC+TGG + chr2.1:17893682-17893701 None:intergenic 40.0%
TTCGCTGCTGTATACGAATA+TGG + chr2.1:17894322-17894341 None:intergenic 40.0%
TTGTAATGGTACAGTTGTCC+AGG - chr2.1:17894430-17894449 MS.gene069828:intron 40.0%
! GATATGCAGTCATTCTGGAA+AGG - chr2.1:17893396-17893415 MS.gene069828:intron 40.0%
!! TGTGTCTACCTTTTTAGTCC+AGG - chr2.1:17894580-17894599 MS.gene069828:intron 40.0%
!! TTGGGTTTTATCGTGTGTGA+AGG - chr2.1:17893937-17893956 MS.gene069828:intron 40.0%
GACAGACAACACGCATCAAT+AGG + chr2.1:17893356-17893375 None:intergenic 45.0%
GCACAAATTCCTACTGCCTA+CGG - chr2.1:17893308-17893327 MS.gene069828:CDS 45.0%
TCAGCATTTGCCTCAGCAAA+AGG + chr2.1:17894266-17894285 None:intergenic 45.0%
TTCAACTTCAAGGCGATCAG+AGG - chr2.1:17894552-17894571 MS.gene069828:intron 45.0%
! TTTTGCTGAGGCAAATGCTG+AGG - chr2.1:17894265-17894284 MS.gene069828:intron 45.0%
!!! AGTATTTTGGGGTCTTGAGG+TGG - chr2.1:17894027-17894046 MS.gene069828:intron 45.0%
!!! TTTTTCTGGGGTGCTTAACG+TGG - chr2.1:17893885-17893904 MS.gene069828:intron 45.0%
AGACATACCTGTCCTTGCTC+TGG + chr2.1:17894458-17894477 None:intergenic 50.0%
ATTGTTGCCACACATCCCAC+AGG + chr2.1:17894236-17894255 None:intergenic 50.0%
GACATACCTGTCCTTGCTCT+GGG + chr2.1:17894457-17894476 None:intergenic 50.0%
TATGACTCCTGTGGGATGTG+TGG - chr2.1:17894226-17894245 MS.gene069828:intron 50.0%
TCCAACTTACCGTAGGCAGT+AGG + chr2.1:17893320-17893339 None:intergenic 50.0%
TCCTACTGCCTACGGTAAGT+TGG - chr2.1:17893316-17893335 MS.gene069828:CDS 50.0%
! CTCCTTTGATGACCCAGCAC+CGG + chr2.1:17894294-17894313 None:intergenic 55.0%
GAGGCAAATGCTGAGGACGC+CGG - chr2.1:17894272-17894291 MS.gene069828:intron 60.0%
GTTGTCCAGGACCCAGAGCA+AGG - chr2.1:17894443-17894462 MS.gene069828:intron 60.0%
AATGCTGAGGACGCCGGTGC+TGG - chr2.1:17894278-17894297 MS.gene069828:intron 65.0%
ATGCTGAGGACGCCGGTGCT+GGG - chr2.1:17894279-17894298 MS.gene069828:intron 65.0%
CCAGGACCCAGAGCAAGGAC+AGG - chr2.1:17894448-17894467 MS.gene069828:intron 65.0%
! CCTGTCCTTGCTCTGGGTCC+TGG + chr2.1:17894451-17894470 None:intergenic 65.0%
! CGCCGGTGCTGGGTCATCAA+AGG - chr2.1:17894289-17894308 MS.gene069828:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 17893293 17894634 17893293 ID=MS.gene069828
chr2.1 mRNA 17893293 17894634 17893293 ID=MS.gene069828.t1;Parent=MS.gene069828
chr2.1 exon 17894598 17894634 17894598 ID=MS.gene069828.t1.exon1;Parent=MS.gene069828.t1
chr2.1 CDS 17894598 17894634 17894598 ID=cds.MS.gene069828.t1;Parent=MS.gene069828.t1
chr2.1 exon 17893458 17893666 17893458 ID=MS.gene069828.t1.exon2;Parent=MS.gene069828.t1
chr2.1 CDS 17893458 17893666 17893458 ID=cds.MS.gene069828.t1;Parent=MS.gene069828.t1
chr2.1 exon 17893293 17893379 17893293 ID=MS.gene069828.t1.exon3;Parent=MS.gene069828.t1
chr2.1 CDS 17893293 17893379 17893293 ID=cds.MS.gene069828.t1;Parent=MS.gene069828.t1
Gene Sequence

>MS.gene069828

ATGTCTGAATTAGACGCACAAATTCCTACTGCCTACGGTAAGTTGGAACTCTATACTATACCTATTGATGCGTGTTGTCTGTCAGATTAATTGTTCTGTTCATGATATGCAGTCATTCTGGAAAGGGTAGTCAATAGTTGTTCAATTATACTGTGTACTGTTGGGTTCCTTGAAAATGCTGTCTCAGATTATTAACATTAGCATATCTTTTGTTTTGAATTTTTATTATCTTTAGGTTTTCATTGTTTATACCTGACAATTGACTAGTAAATTCTATTTTCTCTTTTATGTTAAAACGTAGATGTATTATCTAAACTCCAGAAATGAGAAATTCTTCTATTAACTCTTATGTTAAAATGTAGATGCATTATACCTTCAACTAAACTCCAGAAATGAGTTGCCTTCTTACTGATATTGCTTTTACTTTTACTTGGACATGATGTTGACCCTATTTTTTTTTACTTATAACGATAATCAGTGTGTTAATAGTATTGTTCTTGCAAATGTTAAATGTTAATTAGATGTCATTTCATTATTTAACGAGATGCTTAAAAGATGCCAAGAATCACACAGAATTTTTTTCTAAAATAGTTTTTTCTGGGGTGCTTAACGTGGTTTAAGATTGGGTTTTTAATGTTTATACTTTGGGTTTTATCGTGTGTGAAGGTTTCGTTCCATTATTGAACAAGATGCTTAAAAGATGCCAATCATTGTACATAACTTTGATCTGAAATAGTATTTTGGGGTCTTGAGGTGGTTTAAAATTGGATTTTTAAAGCTTAGGATCTTAACGTTTGTGAAGGGTAAAAATGTAAAATTGATACTTACAGCCTCACACAGCATTCATTATGTAGATGAAGAATAGAAATAGATGGTTTCTGTGTGATCATGATAATTAAGTTTGACTATTGATTATCTTACTTTGATATGATATATGACTCCTGTGGGATGTGTGGCAACAATTTTAGATCCTTTTGCTGAGGCAAATGCTGAGGACGCCGGTGCTGGGTCATCAAAGGAGTACGTCCATATTCGTATACAGCAGCGAAATGGTAGAAAAAGCTTGACAACTGTCCAGGGATTGAAGAAAGAATTCAGTTACAACAAGATTCTGAAAGACCTTAAGAAGGAGTTTTGTTGTAATGGTACAGTTGTCCAGGACCCAGAGCAAGGACAGGTATGTCTTGATGTTCTCTTGTTAGATAGCGATCTATCTACCCCTTGTTGCTTCAAATAACATTTTTTTATGGTGTAGGTTATTCAACTTCAAGGCGATCAGAGGAAAAATGTGTCTACCTTTTTAGTCCAGGTAATGAATGACTTCACATCTTTGGTTTTATGA

Protein sequence

>MS.gene069828.t1

MSELDAQIPTAYDPFAEANAEDAGAGSSKEYVHIRIQQRNGRKSLTTVQGLKKEFSYNKILKDLKKEFCCNGTVVQDPEQGQVIQLQGDQRKNVSTFLVQVMNDFTSLVL