AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060669

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060669.t1 MTR_2g078390 92.076 631 38 3 1 624 1 626 0.0 1142
MS.gene060669.t1 MTR_2g078390 92.272 427 23 2 1 420 1 424 0.0 753
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060669.t1 AT1G53200 32.897 611 369 13 23 620 29 611 2.07e-91 295
MS.gene060669.t1 AT1G53200 34.157 445 255 12 189 620 53 472 3.06e-67 227

Find 123 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAACAACCGTTTCAAATTT+TGG 0.046983 2.2:-16252941 MS.gene060669:CDS
TCACAAATCTTTATGATATT+TGG 0.111730 2.2:-16251822 MS.gene060669:CDS
TGCTCCATTCAAAATGTAAA+AGG 0.178752 2.2:-16243688 MS.gene060669:CDS
CAATGATTTATTGCGATTCT+TGG 0.202579 2.2:-16243416 MS.gene060669:CDS
GGAAACAATCATATCTTTAT+TGG 0.207231 2.2:+16250074 None:intergenic
TTCTACGAGCTGTGGTATTC+TGG 0.211645 2.2:-16250333 MS.gene060669:CDS
ATGGGTGATGGGCAAATTTC+TGG 0.238821 2.2:-16250108 MS.gene060669:CDS
ATGCTCCATACCTGAATTAA+TGG 0.243542 2.2:+16249408 None:intergenic
CTATGGTCTTGAATCTCTTC+TGG 0.293428 2.2:-16243818 MS.gene060669:CDS
ACTGATCTCTGTGCAGGATA+TGG 0.296067 2.2:+16249511 None:intergenic
AATCTGAGTCACATTGATAT+TGG 0.321184 2.2:+16250139 None:intergenic
TAAAGATTTGTGATCCTTGT+TGG 0.321614 2.2:+16251832 None:intergenic
AGCATTACTTCCATTAATTC+AGG 0.321669 2.2:-16249418 MS.gene060669:intron
TGCTCTGTTCTGAGAGGTTT+AGG 0.347230 2.2:+16253077 None:intergenic
GGGCAAATTTCTGGGGAGAT+TGG 0.347692 2.2:-16250099 MS.gene060669:CDS
GATATTTGGTCAAAGAAGAT+TGG 0.350852 2.2:-16251808 MS.gene060669:CDS
TTTGAGTCAAATATTAAAAC+TGG 0.351863 2.2:-16243544 MS.gene060669:CDS
GACTCGTTAATCAGCAAGAT+TGG 0.359537 2.2:-16253025 MS.gene060669:CDS
CCGTTTGTGAGTTCTCTTGA+CGG 0.362937 2.2:+16250268 None:intergenic
CGTCCTTTCTCTTGCGGCGA+GGG 0.365479 2.2:+16253189 None:intergenic
ACTTATAAAGTTTCACCAAA+TGG 0.372174 2.2:-16243961 MS.gene060669:CDS
CAGACAGCTGAGATTCTGTT+TGG 0.375487 2.2:+16251093 None:intergenic
TGATATGTTGCAGGGTACTA+TGG 0.378590 2.2:-16243835 MS.gene060669:intron
CGGCGAGGGGTTGGTTTCGT+TGG 0.383582 2.2:+16253203 None:intergenic
CATACTGATGATCTTGATCT+TGG 0.388425 2.2:-16249949 MS.gene060669:intron
TCTCTCCACTTAAATTCATC+TGG 0.389703 2.2:+16250306 None:intergenic
AGGGAACCTCATTCAGCAAC+AGG 0.396382 2.2:-16250245 MS.gene060669:CDS
AGGGAATCTCATTCAGCAAC+AGG 0.401600 2.2:-16250212 MS.gene060669:CDS
GAGCAGGTATGCAGTTCAAT+TGG 0.404846 2.2:-16251153 MS.gene060669:CDS
TGCAGAAAATTATTTACGTC+TGG 0.413203 2.2:-16249469 MS.gene060669:CDS
AATATTTATAGAGGGTATAT+CGG 0.416338 2.2:-16243626 MS.gene060669:CDS
AATTTGAAACGGTTGTTGTT+GGG 0.419255 2.2:+16252945 None:intergenic
TCTACGAGCTGTGGTATTCT+GGG 0.420238 2.2:-16250332 MS.gene060669:CDS
CATATTCTGTACTCCCAATC+AGG 0.422877 2.2:-16248003 MS.gene060669:intron
AGGGTATATCGGCAAAATCT+TGG 0.424429 2.2:-16243615 MS.gene060669:CDS
ATCTGAGTCACATTGATATT+GGG 0.426327 2.2:+16250140 None:intergenic
TTTCTCTTGCGGCGAGGGGT+TGG 0.426726 2.2:+16253194 None:intergenic
CCCATCATCATCTTTGAAAC+GGG 0.427031 2.2:+16250363 None:intergenic
TGGGTGATGGGCAAATTTCT+GGG 0.427046 2.2:-16250107 MS.gene060669:CDS
CTTATAAAGTTTCACCAAAT+GGG 0.431244 2.2:-16243960 MS.gene060669:intron
AAACCTCCTACCTCAATGAT+TGG 0.431981 2.2:+16250012 None:intergenic
TGACAGCTGCTGTTGATTAG+AGG 0.432399 2.2:-16248073 MS.gene060669:intron
AAATTTGAAACGGTTGTTGT+TGG 0.436770 2.2:+16252944 None:intergenic
TACGAATGAAGGTACCCATT+TGG 0.438397 2.2:+16243946 None:intergenic
TAAAGATATGATTGTTTCCA+TGG 0.440531 2.2:-16250070 MS.gene060669:CDS
GATTCATCTGTCATGGGTGA+TGG 0.440687 2.2:-16250120 MS.gene060669:CDS
ATTCAGCAACAGGGTACATA+TGG 0.446434 2.2:-16250235 MS.gene060669:CDS
ATAAGGGATGTGTACCTGAT+TGG 0.450077 2.2:+16247989 None:intergenic
CATTATAAATATACCTCTAC+TGG 0.452130 2.2:+16251769 None:intergenic
TAAGGGATGTGTACCTGATT+GGG 0.452135 2.2:+16247990 None:intergenic
CAGAATCTCAGCTGTCTGTA+TGG 0.452523 2.2:-16251088 MS.gene060669:intron
ATGTAGACGTGTGGTCTTCA+AGG 0.456750 2.2:+16243710 None:intergenic
ATTTAGGGAAACTTGATTTG+TGG 0.458709 2.2:-16249578 MS.gene060669:intron
AACCCCTCGCCGCAAGAGAA+AGG 0.461280 2.2:-16253192 MS.gene060669:CDS
ACCCATCATCATCTTTGAAA+CGG 0.462419 2.2:+16250362 None:intergenic
GTTCTTAGTGTTTACTTGAA+AGG 0.465759 2.2:-16252987 MS.gene060669:CDS
GGAATCTTCGCTGTCGGTGG+TGG 0.466273 2.2:-16243594 MS.gene060669:CDS
CCCGTTTCAAAGATGATGAT+GGG 0.470053 2.2:-16250363 MS.gene060669:CDS
CTCTCAGAACAGAGCATCGC+CGG 0.472530 2.2:-16253070 MS.gene060669:CDS
AAGGTCTTGTAAGTGATAAA+CGG 0.480340 2.2:+16253120 None:intergenic
TGGTTTCGTTGGAGTAATGT+TGG 0.483320 2.2:+16253214 None:intergenic
GCATGTGCATCGTACATGTA+TGG 0.486793 2.2:-16243493 MS.gene060669:CDS
GCTAAAGAATAACGACATGT+TGG 0.495921 2.2:+16243987 None:intergenic
TCCCGTTTCAAAGATGATGA+TGG 0.496382 2.2:-16250364 MS.gene060669:CDS
ACTCGTTAATCAGCAAGATT+GGG 0.501434 2.2:-16253024 MS.gene060669:CDS
CATTAAACTGATCTCTGTGC+AGG 0.506396 2.2:+16249505 None:intergenic
GAGCACAAGAGAAATTCATA+TGG 0.511099 2.2:-16243391 MS.gene060669:CDS
CATTCAAAATGTAAAAGGGC+AGG 0.516124 2.2:-16243683 MS.gene060669:CDS
AGAATACCCAAAATTTGAAA+CGG 0.524639 2.2:+16252934 None:intergenic
GCTCCATTCAAAATGTAAAA+GGG 0.524806 2.2:-16243687 MS.gene060669:CDS
GGATTCCAGATGAATTTAAG+TGG 0.524814 2.2:-16250311 MS.gene060669:CDS
CAGAATATGAACTATTGCAT+TGG 0.528562 2.2:+16248017 None:intergenic
CAGATGCAGAATACAATACA+TGG 0.529334 2.2:-16243768 MS.gene060669:CDS
TGTAGACGTGTGGTCTTCAA+GGG 0.535444 2.2:+16243711 None:intergenic
ATTCATCTGTCATGGGTGAT+GGG 0.535729 2.2:-16250119 MS.gene060669:CDS
TTGTTGTTCCGTCTTATGCA+AGG 0.541275 2.2:-16251125 MS.gene060669:CDS
ATCTCATTCAGCAACAGGGT+TGG 0.545631 2.2:-16250207 MS.gene060669:CDS
CATCCAATCATTGAGGTAGG+AGG 0.550659 2.2:-16250015 MS.gene060669:CDS
TCACGCAGACCGTTCCCCTC+CGG 0.553656 2.2:+16249537 None:intergenic
GTCCTTTCTCTTGCGGCGAG+GGG 0.554539 2.2:+16253190 None:intergenic
CAGCAACAGGGTACATATGG+AGG 0.556031 2.2:-16250232 MS.gene060669:CDS
CAGAGACGAAGAACGTAAGA+AGG 0.556102 2.2:+16253101 None:intergenic
ATGGTTAGCATCAACATCCA+TGG 0.556205 2.2:+16250053 None:intergenic
TGACTCAGATTCATCTGTCA+TGG 0.560390 2.2:-16250127 MS.gene060669:CDS
ATGTACCCTGTTGCTGAATG+AGG 0.560495 2.2:+16250239 None:intergenic
CTTGGAATCTTCGCTGTCGG+TGG 0.563284 2.2:-16243597 MS.gene060669:CDS
ACAGGGTTGGACAGTCAGAA+TGG 0.565846 2.2:-16250194 MS.gene060669:CDS
TTCAAAATGTAAAAGGGCAG+GGG 0.566206 2.2:-16243681 MS.gene060669:CDS
TCGTCCTTTCTCTTGCGGCG+AGG 0.566647 2.2:+16253188 None:intergenic
ATTCAAAATGTAAAAGGGCA+GGG 0.568958 2.2:-16243682 MS.gene060669:CDS
CAAGATTGGGTTGCAGCTAG+TGG 0.569050 2.2:-16253011 MS.gene060669:CDS
GTTCCCCTCCGGAAAACGCA+AGG 0.580836 2.2:+16249548 None:intergenic
AGATTGGATTAATGAAGACT+TGG 0.581929 2.2:-16251792 MS.gene060669:CDS
CCGTCAAGAGAACTCACAAA+CGG 0.588500 2.2:-16250268 MS.gene060669:CDS
AAAATCACATCCAATCATTG+AGG 0.588856 2.2:-16250022 MS.gene060669:CDS
TAGCATCGTCCTTTCTCTTG+CGG 0.592844 2.2:+16253183 None:intergenic
CTAACCACCAACTCTCCGCC+CGG 0.596614 2.2:-16253163 MS.gene060669:CDS
TCACATCCAATCATTGAGGT+AGG 0.599389 2.2:-16250018 MS.gene060669:CDS
AATGAAGACTTGGCCAGTAG+AGG 0.600519 2.2:-16251782 MS.gene060669:intron
AGAGTTCAGCTATGTTGTGA+AGG 0.602120 2.2:-16243467 MS.gene060669:CDS
GCAGCAAATACTGCAATCTC+CGG 0.603469 2.2:+16253051 None:intergenic
AATCTTGGAATCTTCGCTGT+CGG 0.606501 2.2:-16243600 MS.gene060669:CDS
CGGCGATGCTCTGTTCTGAG+AGG 0.610034 2.2:+16253071 None:intergenic
AGTATGCAAGATATCTTGTG+TGG 0.617781 2.2:+16249966 None:intergenic
AGCAACAGGGTACATATGGA+GGG 0.617900 2.2:-16250231 MS.gene060669:CDS
TAGCATCGCCTTGCATAAGA+CGG 0.619281 2.2:+16251117 None:intergenic
TTCCCCTCCGGAAAACGCAA+GGG 0.629792 2.2:+16249549 None:intergenic
AAGTAATGCTGCTGATGCAA+AGG 0.630805 2.2:+16249431 None:intergenic
TAATTCAGTTGAGTCGCACA+TGG 0.631264 2.2:-16250169 MS.gene060669:CDS
TGCAGAATACAATACATGGA+AGG 0.632136 2.2:-16243764 MS.gene060669:CDS
GGGTGATGGGCAAATTTCTG+GGG 0.633440 2.2:-16250106 MS.gene060669:CDS
GACTCAGATTCATCTGTCAT+GGG 0.637315 2.2:-16250126 MS.gene060669:CDS
CAAGAGAACTCACAAACGGA+GGG 0.637632 2.2:-16250264 MS.gene060669:CDS
GTGATTTACAGTTACTCACA+TGG 0.648324 2.2:-16243522 MS.gene060669:CDS
GTATGCAAGATATCTTGTGT+GGG 0.649348 2.2:+16249967 None:intergenic
GGGAATCTCATTCAGCAACA+GGG 0.655502 2.2:-16250211 MS.gene060669:CDS
GTTTGACATTCTACGAGCTG+TGG 0.664988 2.2:-16250341 MS.gene060669:CDS
TCAAGAGAACTCACAAACGG+AGG 0.667122 2.2:-16250265 MS.gene060669:CDS
GGAGATTGCAGTATTTGCTG+CGG 0.680529 2.2:-16253049 MS.gene060669:CDS
GGGAACCTCATTCAGCAACA+GGG 0.682719 2.2:-16250244 MS.gene060669:CDS
GAATGGAGCATGTAGACGTG+TGG 0.691338 2.2:+16243701 None:intergenic
GTAGACGTGTGGTCTTCAAG+GGG 0.694535 2.2:+16243712 None:intergenic
ACTATTATATGAATCCAACA+AGG 0.729245 2.2:-16251846 MS.gene060669:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr2.2 gene 16243366 16253273 16243366 ID=MS.gene060669
chr2.2 mRNA 16243366 16253273 16243366 ID=MS.gene060669.t1;Parent=MS.gene060669
chr2.2 exon 16252941 16253273 16252941 ID=MS.gene060669.t1.exon1;Parent=MS.gene060669.t1
chr2.2 CDS 16252941 16253273 16252941 ID=cds.MS.gene060669.t1;Parent=MS.gene060669.t1
chr2.2 exon 16251783 16251899 16251783 ID=MS.gene060669.t1.exon2;Parent=MS.gene060669.t1
chr2.2 CDS 16251783 16251899 16251783 ID=cds.MS.gene060669.t1;Parent=MS.gene060669.t1
chr2.2 exon 16251089 16251174 16251089 ID=MS.gene060669.t1.exon3;Parent=MS.gene060669.t1
chr2.2 CDS 16251089 16251174 16251089 ID=cds.MS.gene060669.t1;Parent=MS.gene060669.t1
chr2.2 exon 16249950 16250413 16249950 ID=MS.gene060669.t1.exon4;Parent=MS.gene060669.t1
chr2.2 CDS 16249950 16250413 16249950 ID=cds.MS.gene060669.t1;Parent=MS.gene060669.t1
chr2.2 exon 16249419 16249594 16249419 ID=MS.gene060669.t1.exon5;Parent=MS.gene060669.t1
chr2.2 CDS 16249419 16249594 16249419 ID=cds.MS.gene060669.t1;Parent=MS.gene060669.t1
chr2.2 exon 16248004 16248089 16248004 ID=MS.gene060669.t1.exon6;Parent=MS.gene060669.t1
chr2.2 CDS 16248004 16248089 16248004 ID=cds.MS.gene060669.t1;Parent=MS.gene060669.t1
chr2.2 exon 16243961 16244094 16243961 ID=MS.gene060669.t1.exon7;Parent=MS.gene060669.t1
chr2.2 CDS 16243961 16244094 16243961 ID=cds.MS.gene060669.t1;Parent=MS.gene060669.t1
chr2.2 exon 16243366 16243844 16243366 ID=MS.gene060669.t1.exon8;Parent=MS.gene060669.t1
chr2.2 CDS 16243366 16243844 16243366 ID=cds.MS.gene060669.t1;Parent=MS.gene060669.t1
Gene Sequence

>MS.gene060669

ATGGAAGTCGAAGCGTTAGATATAATACAAGCAGATACCAACATTACTCCAACGAAACCAACCCCTCGCCGCAAGAGAAAGGACGATGCTAACCACCAACTCTCCGCCCGGAAAAAGAAGGAGATGAGAATCCGTTTATCACTTACAAGACCTTCTTACGTTCTTCGTCTCTGTCCTAAACCTCTCAGAACAGAGCATCGCCGGAGATTGCAGTATTTGCTGCGGAGACTCGTTAATCAGCAAGATTGGGTTGCAGCTAGTGGTGTTCTTAGTGTTTACTTGAAAGGAACTGTTAACGATGCTTCTCCCAACAACAACCGTTTCAAATTTTGGGTATTCTTCATCTTTCTTGTTTCTGAATATCCCAAATTTCTTCAATTAAGTTTAGAATGATTATGCTATTCTTTTAAATGATTATGCTGTTGTTTGAAAGATTTAACAATTTAGTTTAGAGATTAATTTCTATGCACTGATGGTGTAAAACTTGTTTACACATGCATCCAATGCCATTTTTATTGGTTAGTTTTTAAAATATCTCAACAAATGTCTACATGTTGTAATTGAATGGATGAATGTGTAAAAAAGTTTTACACTGTCGATGTATACAAATTATATTGATACGTTTAGAGGTTAATTTATATGCACAGACAGTGTAATTTTTTATAGGTTGGGATAAAATATACAAATTATATTGATACGTTTAGACGTTTAGATGTATACAATCAAGTCACAATGTAGTGCCATTTTTATAGGTTGGTTTATAAAATATATCCACAAATGTCTATATCACGTAAATTGATTGGATGCATGTGTTAAAAAGTTTTACACTGTCGGTGTATACAAATTCTACTGACATCTGATAGGAGCTCTCTCATAACATCAATTTTAAGATACATTTACAAAAGTGGAATGGCATGGCGAAACAGTAGATTTCTATTGGATGTCAGTGGAAAACTGGTTTGCTCTGACCGTGTATATCCTTTAAATTTAAAGGGGATTGAATCGATTTGGAAAAGCTTTGTAGCACTTATATGAAAAATAAATGTTTTTAATTTTTGTTGTAAACCATGGGTTTAATCATGTAGGAATACTTCATGTTTGAGGAGTTGTGAGACACTTGATATGGGCACCTATTTGGACATTTGAGCTGTGGTTTGCCATGTTATGGAAGACTAGCTCCTTTTGCTATAACTGACTAGTATTTGTATAGTTAACCACATGTTGAAATCATATTACTTTACGCATAGATTGAAACCAGCCTCCCTTACTTCTGTTACCTTTTACCCCCTCTTTCTAAAGTTGTTATACATTTTTGCTGTAGTTTCTTTCATCATGGATATCCTGAATCCAAAATGTATGCCTGTGTATATGTAGGTTTTACTCGAGCTTATTAAGCATGTGAAAAACTATTATATGAATCCAACAAGGATCACAAATCTTTATGATATTTGGTCAAAGAAGATTGGATTAATGAAGACTTGGCCAGTAGAGGTATATTTATAATGGCTACCTTTATAATACTATTTTGAATAGGTATTTGAAAAATTCTTGCATATGCAGCTATGCTCGTCTTTTCACATACTAACATTATCTTCTACAATATTCAAGATCTTGATGGCTGAGTTCTAACCAAATTCCAACTTTAATGCTCAGTGTTCATTACCATCTATTAAGTTTTTTTTTTTTTCCTTCACTTTTTTCTACTTTAGCACAAAGGTTACAATTTTATAACAGATTAGCTGAATTTTACATGCAGAAAATTCTTTCAGTTCATTATTGAAGCTTAACTTTCAAAGATTCCCTAAATTTTAGATGTAATTCTTAGTAATTTTGTAAAGATTTCTTATTAATTTATTGCATGTGCAAGCTAAGTGAGAGTAATCAGTGTTGTGTTTAATGATTATATGTTTGTTCCTATTTACATTTTGTTGCAGCCTGCTGTTGCACAACTCTTCTATATCCAATGCAAATAATCTCACCAAATCTCTTATAACTTCAGTTCTAGTTGATAAATAACTTATATTTGCAATTGAAAAACTGTTATGGGTAGGTTGATTTAGAACATTCAAATTTATTGTGTATTTTTACTTGGAAAGCAGAGCAGGTATGCAGTTCAATTGGAGTTCTTGTTGTTCCGTCTTATGCAAGGCGATGCTAACCAAACAGAATCTCAGCTGTCTGTATGGTATGCTATATTAAATTTTAATGCTTAGCTCTTTACCTGTAGGGGAGCCTTGGCACAATGATTATATTTGCTATTGTGTAACTACTAGAAAGTCATAGGTTTGAGTAATGAAACCAAATGGATCCGACTCTTACTGAACATTGGCGTGGAAAGAGCGGCTAGTTTCAGTAGAGACATATGTGTTGCATCATGATGCCAAGGAGTGAATTGTTTCAAAACTTGTCATCACAAGATAATGTGGTCAATTGATAATGTGGTCAATTGGTTATTTACCATTACCGTTCTATTCTTTAGCGGGTGATATATTGTTCTGTAAAAGTTATATTGCTCATAATTGTTGATGAGGTATTAATGATTATCGCCATTAGTATTCTAATTCTCAAAAAATTTAGGAATAATACCTTAACTAAGAATATGTATGGTTCTCAACTATTCATTATGAAATACTGACGACTTATAACTTAACTGATAATGACTTAAACACCTTTGACTTTTTTCAATTGCTAACTTTCCTTGATAAAGCATTTTGTCCTGCATTAATGACGATTTTTACTTCAAAAATAAATTGCACCTTTTGAGAAATACTGTTATAAGTATGGTTGCTTAGTTGGCTCTAATTTTGTCCTGCATCTTATCCCCTGGTCTTTTTTTTTTGTCCTTTTGTGTCTTCATTAGCATTGAGCAAGAGAAAACTGATAATGATCCCGTTTCAAAGATGATGATGGGTTTGACATTCTACGAGCTGTGGTATTCTGGGATTCCAGATGAATTTAAGTGGAGAGATTTAGATCAAATTGACCGTCAAGAGAACTCACAAACGGAGGGAACCTCATTCAGCAACAGGGTACATATGGAGGGAATCTCATTCAGCAACAGGGTTGGACAGTCAGAATGGCATAATTCAGTTGAGTCGCACATGGCAGACTCCCAATATCAATGTGACTCAGATTCATCTGTCATGGGTGATGGGCAAATTTCTGGGGAGATTGGCACCAATAAAGATATGATTGTTTCCATGGATGTTGATGCTAACCATAGAAGAGAAAAATCACATCCAATCATTGAGGTAGGAGGTTTTTCCTTGACATCTGATGAAAATCCCACACAAGATATCTTGCATACTGATGATCTTGATCTTGGTAAGATTTTTGTTTTCTGATTAATCATTAACGGAAGATTATCTATTTCTTGTGGCTGCACACCTGTATGAGAATGTGCATGCACATATGCTTGTATATTTTCAATTTTTCCTATCTGATTTTTTTCTTCCCAGATTGAAAATATTGATTTTGTCTTGCATAATAGTTTGATAAGGCTAGATTTTAATTTTCTCATGCCTGCGATTACTGTTTTACAGCAGCCACTTATTAGTCTTATTCTAAATATTGCTCTTCATTTAGCATCTATACTCTCACATCAGTCTATATTTTAGATAGCGTTTGCTATTCAGCTCACCTGTAACTTTACTTTTGATTTTCTTATGCATTTATTTAGGGAAACTTGATTTGTGGTTGTTACCCTTGCGTTTTCCGGAGGGGAACGGTCTGCGTGAGTTCCATATCCTGCACAGAGATCAGTTTAATGACTACTATAACAATGCAGAAAATTATTTACGTCTGGCTCTAAATTCAACATCCTTTGCATCAGCAGCATTACTTCCATTAATTCAGGTATGGAGCATACTTTTCAATTTCAAATAATTTTGGAACCACTTCACTACTTTTGGTATTGGTATTTGTAATTATTGAATAAACTGAAAAGATGGTTTAAAACTTTGAAGTTATAACAAGTCACTAGTTACATAACCCCATCCCCAAAATAAAACAAAATTTAACTTAGATTTCACTAGTAGTGTAAAACTTTTCTGAACTATGTTATTCAATAATAATATCTGGCCACATCGCAAAAAAAAAAACATTTTTTTTATGTAAATTGATATTCTTTTAAGAAGAAGCCGGGCCAAACAAGTTTTTAAAGAAGATCTAATAATAAGAAGTAGCTTACTTTACATGAGTAAAAACACAACCAGTAGCTTCAATATAAGCCATAAATAGTAACTTTGAAATGGCTTCACTTTAGTGGAGCTTTTGTGCCATAACCTTCCTTCGACTCCTACTCATCATATTTTGTATAATACATTTCGTAATTTTTATTGCTGTCCGTGTAGAAATGTTAAACTCTTATTTCTATTTGACATATACAATTATAAATCCGTTGCCCCGTTCAAATTCATCTACTCTATTTCACATTTTCTAACCTTCCCTTGATGACTTAAGCTTTTGGATCACATCCCTTATAGTGTCTCAAAAACCTTTATTACCAACTGATTAGGAGCTCAATCCTTGCAATTTGCGATCCACATCCATCATTATTTAATTAAATTCCAGTACTTGGATAAGAGGGTGTTTTGGCAAAGCTTATTCGGGTTTTTCTTATGACATAAGTGTTTGTGTAACTGTTTGGAGACTTATTTTCATAAGCTACCTCAGATGTCCTATTTTCTAAAGCTATTTTCAGTGTATGCAGAGTTTATTTTCATTTCCTCTTTGATGGTAAAAACAACTTATTCATAAACTCTTTTACTTAAGTTGTTTATCCAGACATTCTCTAAGTAGGCCGGAGCACAACCTTCAATTTTCTACATGCATTTAAGAGTGTTACTAGTGATTAAAAAAAAAAAAAATTGTAATCTAAACAGACCATTTTTAGTTGTTGGGATACTTGGTCCTTTATTTGACCTTCTCTTCCATATGCATGTCAGCATGTGTACAAGCATAATATGAAAATATAATTGGCTTGGATTATTTGAAATCAATTTTTCATTTTAACGTGCACAAAACAACTATATGTGCTCTGCAACTCTGCTGTATGTTTCCTTTCTGTATGTACATGCACATGCATATTTTCCATCTGCTTGAAACAATATATACGATAATTATTTTCTCCATGGAATAATTTTGAGATATCAACAATTGCTTCACTTTATTTTCTGTTGACAGCTGCTGTTGATTAGAGGTCGAGTTAATGATGTTCTAACTTTACTTGAGAACCAATGCAATAGTTCATATTCTGTACTCCCAATCAGGTACACATCCCTTATCTTATTGTTGAAAGAGAAAACAAGAGTGCCATATGGTTAAAGCGTGTGTTTGAATTATACTCTGGAGTCAATTTTATCTTGGCTCGAAGTAATAATTGCAAAAAATATCGGAGATACTATCCCTATTTACATGATATGATATTCCGATACAATAAAATACAAAGATCGTAATATTAATATGCTGAAATAGCGGCGCTATAGCGCTATTGCATAGCTGTATGTAAGTGCAGAAACAAATCCGAAATGTGTGCACAATATCAGATATACGCAGAAACATATTTAAAAATAGAGCGAATACTAGAGGGAGACCAGAAGCTACCGATCTGAATCAGACAAATATTCACTAGAAAAGACAAAGAAGTTGCTAGTGAGTTGAACTCTGGAATCGGCGTCATAATTGGATCTCTGGAAGGAGGCGTGTGAGTCTCACATGCCGGTGGTCTGGGCACAGCGTAGATGGTTTTACGGCATTCACCGTGCAAGGCGGCAGTCCGACGGTCGCAGCGGCAGCCCTATGAGATTCTGACTTGGTTCTTCTAGAACAAAGAGTCAGCACTTACTGTTGCTGAAAAACCCTAACAAAATTGAGAGAAAAAAATAAACCAGAGCTTGAGATACCAATTAGAATATATTATTAGAATTGCTTGTGAGAAATTACTTTCAGCCTTGTTATTTATAACCAAGTATTTACAATCAAATCCCTTGATAAATAGTGAAGTTAAAACTAAATAGTTACAACTTTTCAACATCTCAACACCCCCCCTCAAGGTGGTACGTATACATAAGTTATCCGAGGACTCCATAGAGATTTAGTAAAGATATCTGCGAGTTGATCCTTGGAATTGACAAATGAGGTTCTAATGATTTCGGTGATAATCTTCTCTCTAAGAAAGTGACAGTCACCTTCAATATGCTTTGTCCTCTCGTGAAAAACTGGATTGGAGGAGAGGTGTAAAGTTGATTGATTATCACATACAAGTTCCATTTGACCAACCTCACAAAAATGTAATTCTTGCAGTAAATGTTTCAACCACACAAGCTCACATGTAGTATGAGCCTTAGCTTGATACTCGGCTTCTGTATTCGACCTGGAGACAACAGTCTGTTTTTTGCTCTTCCATGAAATCAGATTTCCGCCCACAAAAATGCAATAACCAGAAGTAGACCGTCTATCACTTGCATCTCCTGCCCAATCGGCATCTGAATGCCCAACGATATTTGTATGGCCTCTATCCTTTTCCAGGTGCTCTTTTGATGTACTTAGAATTCGACACAAGGAGAGATAAGACTGACTTACGATGCTAACCAGAAAAGATATGTCTGGTCTGGTCAAGGTGAGATAATTCAATTTTCCCACTAGTCTTCGGTATCTGCATGGATAAGAATAGGGCTCCCCCTGGTTAGGAAGAAGCTTTACATTAGGATACATATGAGTATCAACTGGTTTACTATCTATAAGACCTCTTTGCTCCAAAATATCAAGGGCCTACTTCCTCTGAGAGATAGCAATACTAGAGTTTGATTGAGCCACTTCAATCCCGAGAAAGTAACTTAGAGGACCCAAGTCTGTAGTCTGAATGTTTTGAATAGATGTTGTTTTAATGCCTTGATACCTTCATAATCATCACATGTAATTACAATGTCATCAACATAAACCACAAGGTAGATATATTTATTTGATGATGAGCATTTAAGAAAAATAGAATGATCTGATTCGCAACGGGTCATCCCAAGCTGTTGTAGAACTTTGCTGAGTTGACCAAACCATGCCCGAGGTGATTGTTTCAAACCATACAAGAACCTTTTCAATTTGCAAACCGAACTATGCCCCCCGAGCAACAAACCCAGGAGATTGCTCCATATAAATCTCTTCTTCCAAGTCCCCATGCAAAAAAGCATTCTTGATATCCAACAGAAACAAGGGTCAACGTTTCATGGCAGCCATAGCTATAAAGAGACGAACAAATGTTTCGGAACTGGTGAGAAGGTATCACTATAATCTTGACCATATAATACCATGAGAAGCCATGAAGGAGTTAAACGATGTAGAAAAATATTCTTTTGCATTGTCACTCCATAAAATTCAAATTGTTTTACCAAATTGGGTCTTTATCTCTGACCAAAACATTTGAAAGGCACCAAATAATTCTGAACGATCCTTCAATAAGGTGATCCAGGTGCATCTAGAGAAATCATCATTGAAAGTGACATAATATTTGTATCCCAAATTAAACGGCACACGACTAGGACCCCAAACATCAGAATGGATGTTATCAAAGGGAGAGATTGATTTTTTATTGGCACTACTTGGAAAAGATACCCTGACATGTTTGCCAAGCTGACATGATTCACAATCCAGTGGCTTTAAATACGAAAGATGAGGAACTAATACCTTTAACTTAACCAAACTTGGATGACCCAAACGACGATGAATGATATCTGGAGATGTAGAAACACCACAAATAGTGGATGGATGAGAATGGAGATAATAGAGACCATGTGATTCAGATCCTGTTCCAATTGTCTTTCCCGTACTTCGGTCTTGTATAAGGAAAGAGTCATAAGAAAATGTTATAGATCAATTGAGAGATTGAGTAAGTTTACTGACCGAGATCAAATTAAAAGCACTTCCAAGTACGAATAACACATAATTCAAGGGTAGAGATGGCAGAGGTGAAGCTTGACCAATACCAGTAACTTGTGCCTTAGAAGCATTAGCAAGAGTAATGTTGTGAGGTATCTTAGGAGGTGACAAGTTAGAGATAAGAGAGGGATTACCAACTACATGATCAGAAGCACCAGAATCTAAAATCCATAATCCCGTGGTGTGAGATAGGTATACCACATAATTATTGAAATGAGCCAAAGTGGCTGAAGAGGAAACCAGTTGGATCGCCATTATGCAACACGAAACAAAAACCAGGACGGTGCCCTTTGGACACCCAACAACTGAATTATTATATATATATATAGAAACACACACACACACACACTACAATAGATCCACCTTGTTCTATGAAGGAGCATTTTAGCAACCAACACCTTTTCATTTAGACCAAAAACCCAATTTTTTTTTTTAAATATCAAAAGTCAGGGATACAATTGTAATTCAAGTTTGATCATTAATGTTTTTTTTTTTGTTCTGGCTGGGTTGTCTCTCACACATTGATTCTCTCTCTTTCATTCTTCCATCTTTGAAAAGTGAATATTCTCGATTAACGTTTGAAGCCACCGTCGATGTTTTCTCCGATTCCGAACACGTAACATCAAAGAAACTAAGATTCGATTTCCCTGTATTTTTATTATATTCTATTCCTATATTTCCACACGTAGCATCAAAGAAAAATCACTAAAAAAATCAAAGATGTTCTATATTTTTATTATATTCCGAACACGTAACATCAAAGAAACCTGCTGTTTTAAGCGCTTGTCCAGTATGTTTCAATTTGGATTGAAGTTGCAACACCTTCAAATTTAGGCTTCAGGTCCTATAAGCATGGACTCAATATCTTTAGATTCAGGGGAATGATAGAATATAAGGAAAATATATAATATTAGCATGAACTTGAGTTATCTCATAGGATCAATTTCATATTTTAGAACATCTCTTATTATTAGTTTCCTATTATTAATTATTTATTATAATGACTTGTTTTTCAATTAGGATTAGAAGTCTTGTATCCTTATAAATAGGGGTTAGTACTCAATCTTTTTACATCATTGAATTGTCAAACATATTTTAATCATTATTGTTTCTATGGTCTCTTTATCTAAAACCCACTACTTCAGCACGTGCATATAATCATTAAATGAATTTGACAAAAGCAAGTTGTGGTAATTTACTCATATTGCTTTTTCGTTATGCTTATGAAGTTACTGATGTGGTGATTACAGATTACGAGCTATTCTCCTAGAGCATTTTGATCGAAAAAACACTCTCTTGATTCAAAGCTGTTTTGAGGATATATTAAAGAAGGATCCAACATGTCGTTATTCTTTAGCAAAACTTATAAAGTTTCACCAAATGGGTACCTTCATTCGTAACAATTTCAAATAAATTGAATATTTTTTTTTCTTCTTATCTCTGTTTCACTTGCTATAATTTCCTTTCCTTCATTAGTTGGTTTCTTCTGATATGTTGCAGGGTACTATGGTCTTGAATCTCTTCTGGAAATGATTGCTTTACATTTAGATGCTACAGATGCAGAATACAATACATGGAAGGAGCTCTCTGTATGTTTTTCTAAATTCTCTCCCCTTGAAGACCACACGTCTACATGCTCCATTCAAAATGTAAAAGGGCAGGGGCAGCAACATCACTCCTTTCGCCATACCCCAAAAATATTTATAGAGGGTATATCGGCAAAATCTTGGAATCTTCGCTGTCGGTGGTGGCGTACAAAATATTTCAGCAATAGCAAGTTTGAGTCAAATATTAAAACTGGTGATTTACAGTTACTCACATGGAAAGCAGCATGTGCATCGTACATGTATGGACAAGAGTTCAGCTATGTTGTGAAGGCCTATTCTCATTTACAAAAAGAAAATGACAATGATTTATTGCGATTCTTGGATGAGCACAAGAGAAATTCATATGGAATTTACAACAAAAATAGAAAATAG
Protein sequence

>MS.gene060669.t1

MEVEALDIIQADTNITPTKPTPRRKRKDDANHQLSARKKKEMRIRLSLTRPSYVLRLCPKPLRTEHRRRLQYLLRRLVNQQDWVAASGVLSVYLKGTVNDASPNNNRFKFWVLLELIKHVKNYYMNPTRITNLYDIWSKKIGLMKTWPVESRYAVQLEFLLFRLMQGDANQTESQLSVCIEQEKTDNDPVSKMMMGLTFYELWYSGIPDEFKWRDLDQIDRQENSQTEGTSFSNRVHMEGISFSNRVGQSEWHNSVESHMADSQYQCDSDSSVMGDGQISGEIGTNKDMIVSMDVDANHRREKSHPIIEVGGFSLTSDENPTQDILHTDDLDLGKLDLWLLPLRFPEGNGLREFHILHRDQFNDYYNNAENYLRLALNSTSFASAALLPLIQLLLIRGRVNDVLTLLENQCNSSYSVLPIRLRAILLEHFDRKNTLLIQSCFEDILKKDPTCRYSLAKLIKFHQMGYYGLESLLEMIALHLDATDAEYNTWKELSVCFSKFSPLEDHTSTCSIQNVKGQGQQHHSFRHTPKIFIEGISAKSWNLRCRWWRTKYFSNSKFESNIKTGDLQLLTWKAACASYMYGQEFSYVVKAYSHLQKENDNDLLRFLDEHKRNSYGIYNKNRK