Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061380.t1 | XP_003621734.2 | 92.4 | 367 | 28 | 0 | 1 | 367 | 1 | 367 | 9.20E-165 | 589.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061380.t1 | G7KUQ5 | 92.4 | 367 | 28 | 0 | 1 | 367 | 1 | 367 | 6.6e-165 | 589.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050750 | MS.gene061380 | 0.813888 | 2.04E-51 | -1.69E-46 |
| MS.gene050953 | MS.gene061380 | 0.814202 | 1.74E-51 | -1.69E-46 |
| MS.gene052519 | MS.gene061380 | 0.811414 | 7.08E-51 | -1.69E-46 |
| MS.gene053006 | MS.gene061380 | 0.810175 | 1.31E-50 | -1.69E-46 |
| MS.gene053953 | MS.gene061380 | 0.807745 | 4.35E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene061380 | 0.809819 | 1.57E-50 | -1.69E-46 |
| MS.gene054405 | MS.gene061380 | 0.808903 | 2.46E-50 | -1.69E-46 |
| MS.gene055623 | MS.gene061380 | 0.800761 | 1.24E-48 | -1.69E-46 |
| MS.gene055818 | MS.gene061380 | 0.8281 | 1.08E-54 | -1.69E-46 |
| MS.gene058979 | MS.gene061380 | 0.814783 | 1.29E-51 | -1.69E-46 |
| MS.gene060572 | MS.gene061380 | 0.842715 | 2.18E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061380.t1 | MTR_7g022230 | 95.368 | 367 | 17 | 0 | 1 | 367 | 1 | 367 | 0.0 | 714 |
| MS.gene061380.t1 | MTR_3g082850 | 25.316 | 237 | 165 | 5 | 1 | 232 | 5 | 234 | 6.60e-16 | 78.2 |
| MS.gene061380.t1 | MTR_3g082850 | 25.316 | 237 | 165 | 5 | 1 | 232 | 5 | 234 | 7.16e-16 | 78.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061380.t1 | AT1G24265 | 40.149 | 269 | 152 | 6 | 1 | 264 | 1 | 265 | 3.66e-58 | 192 |
| MS.gene061380.t1 | AT1G24265 | 40.149 | 269 | 152 | 6 | 1 | 264 | 1 | 265 | 3.66e-58 | 192 |
| MS.gene061380.t1 | AT1G24267 | 34.605 | 367 | 200 | 10 | 1 | 359 | 1 | 335 | 9.91e-52 | 176 |
| MS.gene061380.t1 | AT1G24267 | 34.783 | 368 | 199 | 11 | 1 | 359 | 1 | 336 | 6.50e-51 | 173 |
| MS.gene061380.t1 | AT1G24265 | 35.197 | 304 | 153 | 7 | 1 | 264 | 1 | 300 | 2.20e-50 | 173 |
| MS.gene061380.t1 | AT1G04960 | 25.862 | 232 | 155 | 7 | 1 | 223 | 1 | 224 | 1.08e-14 | 74.7 |
| MS.gene061380.t1 | AT1G04960 | 23.810 | 210 | 148 | 5 | 19 | 223 | 39 | 241 | 2.47e-11 | 64.7 |
Find 99 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTAGTTCTGGTGGCTTTCT+TGG | 0.198980 | 7.1:+76640034 | MS.gene061380:CDS |
| TATGCAGCAGTGGTCATTAT+TGG | 0.289388 | 7.1:+76634807 | MS.gene061380:CDS |
| GATAAAATCACTTTAATAGA+AGG | 0.293332 | 7.1:+76638688 | MS.gene061380:CDS |
| TTGAAAGATACCCTTGATAT+TGG | 0.304389 | 7.1:-76639383 | None:intergenic |
| ATGCAGCAGTGGTCATTATT+GGG | 0.324473 | 7.1:+76634808 | MS.gene061380:CDS |
| CTAGTTCTGGTGGCTTTCTT+GGG | 0.336790 | 7.1:+76640035 | MS.gene061380:CDS |
| CACCAGTTTCACCATCTTCC+AGG | 0.340313 | 7.1:+76639081 | MS.gene061380:CDS |
| GAAGGTTTATGAATCAATTC+AGG | 0.342315 | 7.1:+76635894 | MS.gene061380:CDS |
| CTTTGCAGTCAGGACCTTCC+AGG | 0.349606 | 7.1:+76639333 | MS.gene061380:CDS |
| TCTTGTATCTGGTACTTTCA+AGG | 0.361589 | 7.1:+76631007 | MS.gene061380:CDS |
| CAGTACTGTTATTAACTACT+TGG | 0.361844 | 7.1:-76640133 | None:intergenic |
| GGGTAGGATGTGGATGCCTT+TGG | 0.375016 | 7.1:+76634829 | MS.gene061380:CDS |
| AGATCTCTCTCATCAGATAT+TGG | 0.376614 | 7.1:-76639953 | None:intergenic |
| CAACACGACACAGAGGAAAT+AGG | 0.382761 | 7.1:+76638540 | MS.gene061380:CDS |
| TCCCTTCACTCTATGATTTG+TGG | 0.393347 | 7.1:-76638834 | None:intergenic |
| CATCGAATCCACACGACTAC+TGG | 0.402244 | 7.1:-76639978 | None:intergenic |
| CACTTTAATAGAAGGGAATC+AGG | 0.402610 | 7.1:+76638696 | MS.gene061380:CDS |
| GCAAATGACTTACAATAGTT+CGG | 0.404220 | 7.1:-76638593 | None:intergenic |
| ATTGTAAATCCAAGTGGAAC+AGG | 0.409925 | 7.1:+76634553 | MS.gene061380:CDS |
| CTTGATATTGGTGTAATAGA+AGG | 0.417272 | 7.1:-76639371 | None:intergenic |
| CTCTACTCCACAGTATATTC+AGG | 0.422270 | 7.1:+76638889 | MS.gene061380:CDS |
| CTGAGAACGCGCAGTGTGAC+AGG | 0.423830 | 7.1:+76640088 | MS.gene061380:CDS |
| ATTATACAGGCTTTGCAGTC+AGG | 0.424003 | 7.1:+76639323 | MS.gene061380:intron |
| CAGGAAGCCGAGGGGAAGAT+AGG | 0.428041 | 7.1:+76638364 | MS.gene061380:intron |
| TAGGATGTGGATGCCTTTGG+TGG | 0.442173 | 7.1:+76634832 | MS.gene061380:CDS |
| CAATGATGCGTTGCTTGATC+AGG | 0.444499 | 7.1:+76633136 | MS.gene061380:CDS |
| AAGATAGGTGGAAGAATTAT+CGG | 0.446531 | 7.1:+76638379 | MS.gene061380:CDS |
| ACCCTGGAAGATGGTGAAAC+TGG | 0.449403 | 7.1:-76639083 | None:intergenic |
| AAGATCAATGAATCTAGTTC+TGG | 0.452565 | 7.1:+76640022 | MS.gene061380:CDS |
| CTTCTATTACACCAATATCA+AGG | 0.467188 | 7.1:+76639372 | MS.gene061380:CDS |
| GTCATTATTGGGGTAGGATG+TGG | 0.469037 | 7.1:+76634819 | MS.gene061380:CDS |
| TCAGATCCGACAGTATTTGA+TGG | 0.482692 | 7.1:-76639529 | None:intergenic |
| TCATTGATCTTGTTTCCTGC+AGG | 0.483863 | 7.1:-76640010 | None:intergenic |
| TTGATCTTGTTTCCTGCAGG+AGG | 0.484112 | 7.1:-76640007 | None:intergenic |
| GATCACAGGTTAGTAACCTT+CGG | 0.487093 | 7.1:+76634488 | MS.gene061380:intron |
| GATCTTGTTTCCTGCAGGAG+GGG | 0.487459 | 7.1:-76640005 | None:intergenic |
| CTGTTATTAACTACTTGGTC+GGG | 0.489966 | 7.1:-76640128 | None:intergenic |
| AGTTTAAATGATGCTTGTAC+AGG | 0.492731 | 7.1:+76635853 | MS.gene061380:CDS |
| TGATCTTGTTTCCTGCAGGA+GGG | 0.500746 | 7.1:-76640006 | None:intergenic |
| AATGATGCTTGTACAGGCAT+AGG | 0.503064 | 7.1:+76635859 | MS.gene061380:CDS |
| TGGTAGCTCAATGGCTGGCA+TGG | 0.504873 | 7.1:-76639060 | None:intergenic |
| TACAGGCATAGGTAAACAGC+TGG | 0.507727 | 7.1:+76635870 | MS.gene061380:CDS |
| ACTGTTATTAACTACTTGGT+CGG | 0.508126 | 7.1:-76640129 | None:intergenic |
| CTGGATCTGTGCCTCCAACT+AGG | 0.508414 | 7.1:+76639488 | MS.gene061380:CDS |
| TAACCTTCGGAAGCAAATAG+AGG | 0.516381 | 7.1:+76634501 | MS.gene061380:CDS |
| TCTTCCACCTATCTTCCCCT+CGG | 0.518512 | 7.1:-76638371 | None:intergenic |
| AGGCTGCTCAAGATATAGCC+TGG | 0.521316 | 7.1:-76639351 | None:intergenic |
| TGTACCTGCGCCGAGGAGAA+TGG | 0.523625 | 7.1:-76630691 | None:intergenic |
| GGCTTCTAAAGAAGGTGGAT+TGG | 0.524365 | 7.1:+76630971 | MS.gene061380:CDS |
| GATCCTCTATTTGCTTCCGA+AGG | 0.525550 | 7.1:-76634504 | None:intergenic |
| CACACCATGGCTCTCTCACT+CGG | 0.526772 | 7.1:+76630660 | None:intergenic |
| GGTGGATCAGCTGACCTAGT+TGG | 0.532601 | 7.1:-76639502 | None:intergenic |
| GGAGCAATGGCTTCTAAAGA+AGG | 0.537633 | 7.1:+76630963 | MS.gene061380:CDS |
| ACCAGTTTCACCATCTTCCA+GGG | 0.538108 | 7.1:+76639082 | MS.gene061380:CDS |
| TAAGCATAACCTGTTCCACT+TGG | 0.542443 | 7.1:-76634562 | None:intergenic |
| TCGATGAAAACCCCTCCTGC+AGG | 0.547660 | 7.1:+76639995 | MS.gene061380:CDS |
| AAAACGCTTACCCTGGAAGA+TGG | 0.549999 | 7.1:-76639092 | None:intergenic |
| AAATACTGTCGGATCTGACC+AGG | 0.554979 | 7.1:+76639534 | MS.gene061380:CDS |
| CTCATATGATGTTTGCAACA+AGG | 0.557317 | 7.1:+76635827 | MS.gene061380:CDS |
| ATAAAATCACTTTAATAGAA+GGG | 0.558423 | 7.1:+76638689 | MS.gene061380:CDS |
| CTTACCGAGAGTTTGCAGAA+TGG | 0.561316 | 7.1:+76638866 | MS.gene061380:CDS |
| CCGACAGTATTTGATGGAGA+TGG | 0.566249 | 7.1:-76639523 | None:intergenic |
| CTCACCATTCTCCTCGGCGC+AGG | 0.567185 | 7.1:+76630687 | MS.gene061380:CDS |
| TGCTCAAGATATAGCCTGGA+AGG | 0.567858 | 7.1:-76639347 | None:intergenic |
| ATCATTATTGTAAATCCAAG+TGG | 0.576815 | 7.1:+76634547 | MS.gene061380:CDS |
| CTTGTTGCAAACATCATATG+AGG | 0.577200 | 7.1:-76635826 | None:intergenic |
| ATCAATGAATCTAGTTCTGG+TGG | 0.578012 | 7.1:+76640025 | MS.gene061380:CDS |
| GGATCAGCTGACCTAGTTGG+AGG | 0.578994 | 7.1:-76639499 | None:intergenic |
| TGCCACAAATCATAGAGTGA+AGG | 0.579848 | 7.1:+76638832 | MS.gene061380:CDS |
| AGAGCCATTCTGCAAACTCT+CGG | 0.583473 | 7.1:-76638870 | None:intergenic |
| GCCACAAATCATAGAGTGAA+GGG | 0.587625 | 7.1:+76638833 | MS.gene061380:CDS |
| GCAGTGGTCATTATTGGGGT+AGG | 0.588721 | 7.1:+76634813 | MS.gene061380:CDS |
| GAAGCCGAGGGGAAGATAGG+TGG | 0.596262 | 7.1:+76638367 | MS.gene061380:CDS |
| CATAGGTAAACAGCTGGGGA+AGG | 0.596495 | 7.1:+76635876 | MS.gene061380:CDS |
| TTCTATTACACCAATATCAA+GGG | 0.598085 | 7.1:+76639373 | MS.gene061380:CDS |
| ATGTGGATGCCTTTGGTGGA+AGG | 0.598433 | 7.1:+76634836 | MS.gene061380:CDS |
| TGATGATTTCAGGAAGCCGA+GGG | 0.598749 | 7.1:+76638355 | MS.gene061380:intron |
| TGAAACTGGTGGTAGCTCAA+TGG | 0.608807 | 7.1:-76639069 | None:intergenic |
| AATGCCCTACCTTCCACCAA+AGG | 0.612201 | 7.1:-76634845 | None:intergenic |
| ACACTGCGCGTTCTCAGCAA+CGG | 0.612630 | 7.1:-76640082 | None:intergenic |
| ACAGTATTTGATGGAGATGG+TGG | 0.613685 | 7.1:-76639520 | None:intergenic |
| TGCAGCAGTGGTCATTATTG+GGG | 0.615945 | 7.1:+76634809 | MS.gene061380:CDS |
| AGGAAGAAAATATGCAGCAG+TGG | 0.626274 | 7.1:+76634797 | MS.gene061380:CDS |
| ACAGGCATAGGTAAACAGCT+GGG | 0.626357 | 7.1:+76635871 | MS.gene061380:CDS |
| GTGATGATTTCAGGAAGCCG+AGG | 0.627990 | 7.1:+76638354 | MS.gene061380:intron |
| CCATCTCCATCAAATACTGT+CGG | 0.628130 | 7.1:+76639523 | MS.gene061380:CDS |
| ACTGGTGGTAGCTCAATGGC+TGG | 0.629514 | 7.1:-76639065 | None:intergenic |
| GCAATGGCTTCTAAAGAAGG+TGG | 0.631408 | 7.1:+76630966 | MS.gene061380:CDS |
| AGATCTCTCCAGTAGTCGTG+TGG | 0.635591 | 7.1:+76639970 | MS.gene061380:CDS |
| GAGAGAGTGTACCTGCGCCG+AGG | 0.645397 | 7.1:-76630698 | None:intergenic |
| GGAAAGCTCACCATTCTCCT+CGG | 0.658989 | 7.1:+76630681 | MS.gene061380:CDS |
| TCAAGCAACGCATCATTGTG+TGG | 0.663243 | 7.1:-76633131 | None:intergenic |
| CACGACACAGAGGAAATAGG+TGG | 0.667004 | 7.1:+76638543 | MS.gene061380:CDS |
| CAGGCATAGGTAAACAGCTG+GGG | 0.669956 | 7.1:+76635872 | MS.gene061380:CDS |
| CTTTCCGAGTGAGAGAGCCA+TGG | 0.684415 | 7.1:-76630664 | None:intergenic |
| TGCAATACAACACGACACAG+AGG | 0.711051 | 7.1:+76638533 | MS.gene061380:CDS |
| CTGGAAGATGGTGAAACTGG+TGG | 0.738406 | 7.1:-76639080 | None:intergenic |
| GTTCATGCCTGAATATACTG+TGG | 0.744829 | 7.1:-76638896 | None:intergenic |
| GATGATTTCAGGAAGCCGAG+GGG | 0.766630 | 7.1:+76638356 | MS.gene061380:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 76630666 | 76640144 | 76630666 | ID=MS.gene061380 |
| chr7.1 | mRNA | 76630666 | 76640144 | 76630666 | ID=MS.gene061380.t1;Parent=MS.gene061380 |
| chr7.1 | exon | 76630666 | 76630708 | 76630666 | ID=MS.gene061380.t1.exon1;Parent=MS.gene061380.t1 |
| chr7.1 | CDS | 76630666 | 76630708 | 76630666 | ID=cds.MS.gene061380.t1;Parent=MS.gene061380.t1 |
| chr7.1 | exon | 76630952 | 76631028 | 76630952 | ID=MS.gene061380.t1.exon2;Parent=MS.gene061380.t1 |
| chr7.1 | CDS | 76630952 | 76631028 | 76630952 | ID=cds.MS.gene061380.t1;Parent=MS.gene061380.t1 |
| chr7.1 | exon | 76633080 | 76633157 | 76633080 | ID=MS.gene061380.t1.exon3;Parent=MS.gene061380.t1 |
| chr7.1 | CDS | 76633080 | 76633157 | 76633080 | ID=cds.MS.gene061380.t1;Parent=MS.gene061380.t1 |
| chr7.1 | exon | 76634496 | 76634574 | 76634496 | ID=MS.gene061380.t1.exon4;Parent=MS.gene061380.t1 |
| chr7.1 | CDS | 76634496 | 76634574 | 76634496 | ID=cds.MS.gene061380.t1;Parent=MS.gene061380.t1 |
| chr7.1 | exon | 76634796 | 76634857 | 76634796 | ID=MS.gene061380.t1.exon5;Parent=MS.gene061380.t1 |
| chr7.1 | CDS | 76634796 | 76634857 | 76634796 | ID=cds.MS.gene061380.t1;Parent=MS.gene061380.t1 |
| chr7.1 | exon | 76635817 | 76635915 | 76635817 | ID=MS.gene061380.t1.exon6;Parent=MS.gene061380.t1 |
| chr7.1 | CDS | 76635817 | 76635915 | 76635817 | ID=cds.MS.gene061380.t1;Parent=MS.gene061380.t1 |
| chr7.1 | exon | 76638367 | 76638456 | 76638367 | ID=MS.gene061380.t1.exon7;Parent=MS.gene061380.t1 |
| chr7.1 | CDS | 76638367 | 76638456 | 76638367 | ID=cds.MS.gene061380.t1;Parent=MS.gene061380.t1 |
| chr7.1 | exon | 76638528 | 76638602 | 76638528 | ID=MS.gene061380.t1.exon8;Parent=MS.gene061380.t1 |
| chr7.1 | CDS | 76638528 | 76638602 | 76638528 | ID=cds.MS.gene061380.t1;Parent=MS.gene061380.t1 |
| chr7.1 | exon | 76638685 | 76638717 | 76638685 | ID=MS.gene061380.t1.exon9;Parent=MS.gene061380.t1 |
| chr7.1 | CDS | 76638685 | 76638717 | 76638685 | ID=cds.MS.gene061380.t1;Parent=MS.gene061380.t1 |
| chr7.1 | exon | 76638830 | 76638910 | 76638830 | ID=MS.gene061380.t1.exon10;Parent=MS.gene061380.t1 |
| chr7.1 | CDS | 76638830 | 76638910 | 76638830 | ID=cds.MS.gene061380.t1;Parent=MS.gene061380.t1 |
| chr7.1 | exon | 76639050 | 76639103 | 76639050 | ID=MS.gene061380.t1.exon11;Parent=MS.gene061380.t1 |
| chr7.1 | CDS | 76639050 | 76639103 | 76639050 | ID=cds.MS.gene061380.t1;Parent=MS.gene061380.t1 |
| chr7.1 | exon | 76639332 | 76639394 | 76639332 | ID=MS.gene061380.t1.exon12;Parent=MS.gene061380.t1 |
| chr7.1 | CDS | 76639332 | 76639394 | 76639332 | ID=cds.MS.gene061380.t1;Parent=MS.gene061380.t1 |
| chr7.1 | exon | 76639487 | 76639555 | 76639487 | ID=MS.gene061380.t1.exon13;Parent=MS.gene061380.t1 |
| chr7.1 | CDS | 76639487 | 76639555 | 76639487 | ID=cds.MS.gene061380.t1;Parent=MS.gene061380.t1 |
| chr7.1 | exon | 76639944 | 76640144 | 76639944 | ID=MS.gene061380.t1.exon14;Parent=MS.gene061380.t1 |
| chr7.1 | CDS | 76639944 | 76640144 | 76639944 | ID=cds.MS.gene061380.t1;Parent=MS.gene061380.t1 |
| Gene Sequence |
| Protein sequence |
