Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061539.t1 | XP_024639340.1 | 98.9 | 188 | 2 | 0 | 1 | 188 | 266 | 453 | 4.90E-106 | 393.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061539.t1 | A0A396HSG0 | 98.9 | 188 | 2 | 0 | 1 | 188 | 266 | 453 | 3.6e-106 | 393.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049958 | MS.gene061539 | 0.841984 | 3.41E-58 | -1.69E-46 |
| MS.gene050780 | MS.gene061539 | 0.818934 | 1.51E-52 | -1.69E-46 |
| MS.gene051490 | MS.gene061539 | 0.838909 | 2.17E-57 | -1.69E-46 |
| MS.gene051492 | MS.gene061539 | 0.804742 | 1.87E-49 | -1.69E-46 |
| MS.gene053641 | MS.gene061539 | 0.826038 | 3.38E-54 | -1.69E-46 |
| MS.gene054468 | MS.gene061539 | 0.803088 | 4.12E-49 | -1.69E-46 |
| MS.gene05468 | MS.gene061539 | 0.811478 | 6.86E-51 | -1.69E-46 |
| MS.gene058477 | MS.gene061539 | 0.802157 | 6.41E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 37 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCCATTCACACCATATTA+TGG | 0.214195 | 5.4:-52027930 | None:intergenic |
| GATTCAGAATATTTGCAATT+TGG | 0.215007 | 5.4:-52027895 | None:intergenic |
| AGAGGCCTTGTTCTCTCTTT+AGG | 0.218594 | 5.4:+52028118 | MS.gene061539:CDS |
| TTCACACAGGTTTCAGCTTT+TGG | 0.219860 | 5.4:-52028217 | None:intergenic |
| CTTGTTCTCTCTTTAGGTCT+TGG | 0.247801 | 5.4:+52028124 | MS.gene061539:CDS |
| GTGGTCTGCTTCGTGATTAT+CGG | 0.253798 | 5.4:+52028020 | MS.gene061539:CDS |
| TGGTGGAACTTTGGGCTATA+TGG | 0.256656 | 5.4:+52028095 | MS.gene061539:CDS |
| TACTTTCTTGGTGGAACTTT+GGG | 0.281464 | 5.4:+52028087 | MS.gene061539:CDS |
| GTCTTGGGATAAAATCAATA+TGG | 0.303390 | 5.4:+52028140 | MS.gene061539:CDS |
| TTCGAGACAAATGCGCAAAT+TGG | 0.310319 | 5.4:-52028049 | None:intergenic |
| TTGTTCTCTCTTTAGGTCTT+GGG | 0.332936 | 5.4:+52028125 | MS.gene061539:CDS |
| AATAAAGAAACAGCTGGTTT+TGG | 0.354908 | 5.4:+52027998 | MS.gene061539:CDS |
| CATATTATGGATCTAGAAAC+TGG | 0.383191 | 5.4:-52027917 | None:intergenic |
| TCCTCAAGGAGATACTTTCT+TGG | 0.388568 | 5.4:+52028075 | MS.gene061539:CDS |
| ATACTTTCTTGGTGGAACTT+TGG | 0.389611 | 5.4:+52028086 | MS.gene061539:CDS |
| CAGTTTCTAGATCCATAATA+TGG | 0.399797 | 5.4:+52027918 | MS.gene061539:CDS |
| TGGTCTGCTTCGTGATTATC+GGG | 0.429907 | 5.4:+52028021 | MS.gene061539:CDS |
| GATCCATAATATGGTGTGAA+TGG | 0.430360 | 5.4:+52027927 | MS.gene061539:CDS |
| AGCAGATCATCTTGCAAAGA+TGG | 0.464394 | 5.4:+52028312 | MS.gene061539:CDS |
| CTTGCAAAGATGGCTGTTTG+TGG | 0.484608 | 5.4:+52028322 | MS.gene061539:CDS |
| TGAGTAAGTTTGATGAGTCT+CGG | 0.487445 | 5.4:+52028257 | MS.gene061539:CDS |
| TCAAGGAGATACTTTCTTGG+TGG | 0.510759 | 5.4:+52028078 | MS.gene061539:CDS |
| TCTGTAAATAAAGAAACAGC+TGG | 0.514986 | 5.4:+52027992 | MS.gene061539:CDS |
| TCTTGGGATAAAATCAATAT+GGG | 0.519546 | 5.4:+52028141 | MS.gene061539:CDS |
| GATTGTCTTCACAACACTCA+TGG | 0.524206 | 5.4:-52028176 | None:intergenic |
| TTCTTTATTTACAGAACCGT+CGG | 0.577442 | 5.4:-52027984 | None:intergenic |
| AGTCTCGGATTTCTCACTCA+TGG | 0.579409 | 5.4:+52028272 | MS.gene061539:CDS |
| GGCTGTTTGTGGAAATGATG+TGG | 0.583486 | 5.4:+52028333 | MS.gene061539:CDS |
| GAGGAACAAAATATCTAAGA+CGG | 0.590469 | 5.4:+52028413 | MS.gene061539:CDS |
| CAAGACCTAAAGAGAGAACA+AGG | 0.605269 | 5.4:-52028123 | None:intergenic |
| CGTCTTGAATGATATTGCAA+AGG | 0.607459 | 5.4:-52028384 | None:intergenic |
| TTTGTCTCGAAAGCTCCTCA+AGG | 0.608294 | 5.4:+52028061 | MS.gene061539:CDS |
| GAACTTTGGGCTATATGGAG+AGG | 0.625001 | 5.4:+52028100 | MS.gene061539:CDS |
| ACCAAGAAAGTATCTCCTTG+AGG | 0.666708 | 5.4:-52028076 | None:intergenic |
| ATCATTCAAGACGATGCCAG+AGG | 0.688719 | 5.4:+52028394 | MS.gene061539:CDS |
| TGGACAAAACTAAATACCGA+CGG | 0.689514 | 5.4:+52027968 | MS.gene061539:CDS |
| GGTCTGCTTCGTGATTATCG+GGG | 0.768767 | 5.4:+52028022 | MS.gene061539:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GATTCAGAATATTTGCAATT+TGG | - | chr5.4:52027898-52027917 | None:intergenic | 25.0% |
| ! | TCTTGGGATAAAATCAATAT+GGG | + | chr5.4:52028141-52028160 | MS.gene061539:CDS | 25.0% |
| AAACCTGTGTGAAAAAAATC+TGG | + | chr5.4:52028227-52028246 | MS.gene061539:CDS | 30.0% | |
| CATATTATGGATCTAGAAAC+TGG | - | chr5.4:52027920-52027939 | None:intergenic | 30.0% | |
| GAGGAACAAAATATCTAAGA+CGG | + | chr5.4:52028413-52028432 | MS.gene061539:CDS | 30.0% | |
| GTCTTGGGATAAAATCAATA+TGG | + | chr5.4:52028140-52028159 | MS.gene061539:CDS | 30.0% | |
| TCTGTAAATAAAGAAACAGC+TGG | + | chr5.4:52027992-52028011 | MS.gene061539:CDS | 30.0% | |
| TTTCCATTCACACCATATTA+TGG | - | chr5.4:52027933-52027952 | None:intergenic | 30.0% | |
| ! | CAGTTTCTAGATCCATAATA+TGG | + | chr5.4:52027918-52027937 | MS.gene061539:CDS | 30.0% |
| ! | TCTTAGATATTTTGTTCCTC+TGG | - | chr5.4:52028413-52028432 | None:intergenic | 30.0% |
| ! | TTCTTTATTTACAGAACCGT+CGG | - | chr5.4:52027987-52028006 | None:intergenic | 30.0% |
| !! | AATAAAGAAACAGCTGGTTT+TGG | + | chr5.4:52027998-52028017 | MS.gene061539:CDS | 30.0% |
| !!! | TTTCCAGATTTTTTTCACAC+AGG | - | chr5.4:52028233-52028252 | None:intergenic | 30.0% |
| ATACTTTCTTGGTGGAACTT+TGG | + | chr5.4:52028086-52028105 | MS.gene061539:CDS | 35.0% | |
| CGTCTTGAATGATATTGCAA+AGG | - | chr5.4:52028387-52028406 | None:intergenic | 35.0% | |
| GATCCATAATATGGTGTGAA+TGG | + | chr5.4:52027927-52027946 | MS.gene061539:CDS | 35.0% | |
| TACTTTCTTGGTGGAACTTT+GGG | + | chr5.4:52028087-52028106 | MS.gene061539:CDS | 35.0% | |
| TGAGTAAGTTTGATGAGTCT+CGG | + | chr5.4:52028257-52028276 | MS.gene061539:CDS | 35.0% | |
| TGGACAAAACTAAATACCGA+CGG | + | chr5.4:52027968-52027987 | MS.gene061539:CDS | 35.0% | |
| TTGTTCTCTCTTTAGGTCTT+GGG | + | chr5.4:52028125-52028144 | MS.gene061539:CDS | 35.0% | |
| AGCAGATCATCTTGCAAAGA+TGG | + | chr5.4:52028312-52028331 | MS.gene061539:CDS | 40.0% | |
| AGGCTATGTGAAAAATCAGC+TGG | - | chr5.4:52028367-52028386 | None:intergenic | 40.0% | |
| ATGGTGTGAATGGAAAAAGC+CGG | + | chr5.4:52027937-52027956 | MS.gene061539:CDS | 40.0% | |
| CAAGACCTAAAGAGAGAACA+AGG | - | chr5.4:52028126-52028145 | None:intergenic | 40.0% | |
| CTTGTTCTCTCTTTAGGTCT+TGG | + | chr5.4:52028124-52028143 | MS.gene061539:CDS | 40.0% | |
| GAATGGAAAAAGCCGGAATT+CGG | + | chr5.4:52027944-52027963 | MS.gene061539:CDS | 40.0% | |
| GATTGTCTTCACAACACTCA+TGG | - | chr5.4:52028179-52028198 | None:intergenic | 40.0% | |
| TCAAGGAGATACTTTCTTGG+TGG | + | chr5.4:52028078-52028097 | MS.gene061539:CDS | 40.0% | |
| TCCTCAAGGAGATACTTTCT+TGG | + | chr5.4:52028075-52028094 | MS.gene061539:CDS | 40.0% | |
| TTCGAGACAAATGCGCAAAT+TGG | - | chr5.4:52028052-52028071 | None:intergenic | 40.0% | |
| ! | TTCACACAGGTTTCAGCTTT+TGG | - | chr5.4:52028220-52028239 | None:intergenic | 40.0% |
| !! | ACCAAGAAAGTATCTCCTTG+AGG | - | chr5.4:52028079-52028098 | None:intergenic | 40.0% |
| !! | AGTTTTGTCCATCCGAATTC+CGG | - | chr5.4:52027959-52027978 | None:intergenic | 40.0% |
| !! | GTGGAAATGATGTGGTTTTG+TGG | + | chr5.4:52028341-52028360 | MS.gene061539:CDS | 40.0% |
| !!! | AAAGAAACAGCTGGTTTTGG+TGG | + | chr5.4:52028001-52028020 | MS.gene061539:CDS | 40.0% |
| AGTCTCGGATTTCTCACTCA+TGG | + | chr5.4:52028272-52028291 | MS.gene061539:CDS | 45.0% | |
| ATCATTCAAGACGATGCCAG+AGG | + | chr5.4:52028394-52028413 | MS.gene061539:CDS | 45.0% | |
| CTTGCAAAGATGGCTGTTTG+TGG | + | chr5.4:52028322-52028341 | MS.gene061539:CDS | 45.0% | |
| GAACTTTGGGCTATATGGAG+AGG | + | chr5.4:52028100-52028119 | MS.gene061539:CDS | 45.0% | |
| GGCTGTTTGTGGAAATGATG+TGG | + | chr5.4:52028333-52028352 | MS.gene061539:CDS | 45.0% | |
| GTGGTCTGCTTCGTGATTAT+CGG | + | chr5.4:52028020-52028039 | MS.gene061539:CDS | 45.0% | |
| TGGTCTGCTTCGTGATTATC+GGG | + | chr5.4:52028021-52028040 | MS.gene061539:CDS | 45.0% | |
| TGGTGGAACTTTGGGCTATA+TGG | + | chr5.4:52028095-52028114 | MS.gene061539:CDS | 45.0% | |
| TTTGTCTCGAAAGCTCCTCA+AGG | + | chr5.4:52028061-52028080 | MS.gene061539:CDS | 45.0% | |
| !! | AGAGGCCTTGTTCTCTCTTT+AGG | + | chr5.4:52028118-52028137 | MS.gene061539:CDS | 45.0% |
| GGAAAAAGCCGGAATTCGGA+TGG | + | chr5.4:52027948-52027967 | MS.gene061539:CDS | 50.0% | |
| GGTCTGCTTCGTGATTATCG+GGG | + | chr5.4:52028022-52028041 | MS.gene061539:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 52027872 | 52028438 | 52027872 | ID=MS.gene061539 |
| chr5.4 | mRNA | 52027872 | 52028438 | 52027872 | ID=MS.gene061539.t1;Parent=MS.gene061539 |
| chr5.4 | exon | 52027872 | 52028438 | 52027872 | ID=MS.gene061539.t1.exon1;Parent=MS.gene061539.t1 |
| chr5.4 | CDS | 52027872 | 52028438 | 52027872 | ID=cds.MS.gene061539.t1;Parent=MS.gene061539.t1 |
| Gene Sequence |
| Protein sequence |
