Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06156.t1 | XP_003625515.1 | 99.3 | 143 | 1 | 0 | 1 | 143 | 1 | 143 | 6.50E-74 | 286.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06156.t1 | O82201 | 90.8 | 141 | 13 | 0 | 1 | 141 | 1 | 141 | 2.8e-70 | 265.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06156.t1 | G7L0L8 | 99.3 | 143 | 1 | 0 | 1 | 143 | 1 | 143 | 4.7e-74 | 286.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05955 | MS.gene06156 | 0.820968 | 5.18E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene27073 | MS.gene06156 | PPI |
| MS.gene75781 | MS.gene06156 | PPI |
| MS.gene021005 | MS.gene06156 | PPI |
| MS.gene64013 | MS.gene06156 | PPI |
| MS.gene017498 | MS.gene06156 | PPI |
| MS.gene64216 | MS.gene06156 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06156.t1 | MTR_7g100010 | 99.301 | 143 | 1 | 0 | 1 | 143 | 1 | 143 | 4.64e-104 | 293 |
| MS.gene06156.t1 | MTR_8g102180 | 99.301 | 143 | 1 | 0 | 1 | 143 | 1 | 143 | 4.64e-104 | 293 |
| MS.gene06156.t1 | MTR_5g098940 | 42.029 | 138 | 80 | 0 | 3 | 140 | 2 | 139 | 9.50e-39 | 129 |
| MS.gene06156.t1 | MTR_1g025190 | 47.482 | 139 | 73 | 0 | 3 | 141 | 2 | 140 | 1.17e-38 | 128 |
| MS.gene06156.t1 | MTR_5g087820 | 41.791 | 134 | 78 | 0 | 3 | 136 | 26 | 159 | 8.53e-35 | 119 |
| MS.gene06156.t1 | MTR_8g077600 | 37.162 | 148 | 80 | 3 | 3 | 141 | 2 | 145 | 1.15e-30 | 108 |
| MS.gene06156.t1 | MTR_8g077600 | 35.484 | 93 | 47 | 3 | 3 | 86 | 2 | 90 | 1.40e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06156.t1 | AT2G19790 | 90.141 | 142 | 14 | 0 | 1 | 142 | 1 | 142 | 2.48e-96 | 274 |
| MS.gene06156.t1 | AT1G47830 | 48.201 | 139 | 72 | 0 | 3 | 141 | 2 | 140 | 2.48e-39 | 129 |
| MS.gene06156.t1 | AT2G17380 | 41.727 | 139 | 81 | 0 | 3 | 141 | 2 | 140 | 1.62e-38 | 128 |
| MS.gene06156.t1 | AT4G35410 | 41.727 | 139 | 81 | 0 | 3 | 141 | 2 | 140 | 3.23e-37 | 125 |
| MS.gene06156.t1 | AT3G50860 | 39.583 | 144 | 82 | 1 | 3 | 141 | 2 | 145 | 5.28e-35 | 119 |
| MS.gene06156.t1 | AT3G50860 | 39.583 | 144 | 82 | 1 | 3 | 141 | 2 | 145 | 5.57e-35 | 119 |
| MS.gene06156.t1 | AT4G35410 | 45.361 | 97 | 53 | 0 | 3 | 99 | 2 | 98 | 2.05e-25 | 93.2 |
Find 23 sgRNAs with CRISPR-Local
Find 101 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAGAATGAGCTTGCTATTT+TGG | 0.125124 | 4.2:-2969795 | MS.gene06156:intron |
| GAAACCATGGATCGACATTT+TGG | 0.210891 | 4.2:-2969752 | MS.gene06156:CDS |
| GGGGATCCGATTCGTGTTAA+TGG | 0.262612 | 4.2:-2971209 | MS.gene06156:CDS |
| TACGAACAATTTCGCCTTCA+AGG | 0.397557 | 4.2:+2971119 | None:intergenic |
| GCAACTACAAAATTATATAT+AGG | 0.415613 | 4.2:-2970185 | MS.gene06156:CDS |
| GAAGAACGACGAACCCTTGA+AGG | 0.434936 | 4.2:-2971133 | MS.gene06156:CDS |
| TGTTCACCATTAACACGAAT+CGG | 0.437301 | 4.2:+2971203 | None:intergenic |
| TATCATGTTTCATTTAGAAA+AGG | 0.473226 | 4.2:-2969531 | MS.gene06156:CDS |
| TTCATAATACTGAGCAAGAC+GGG | 0.525077 | 4.2:+2971168 | None:intergenic |
| TCTGACTCCTATTCAGCTGA+TGG | 0.529053 | 4.2:-2969444 | MS.gene06156:CDS |
| ACGAACAATTTCGCCTTCAA+GGG | 0.529281 | 4.2:+2971120 | None:intergenic |
| TTCCTGGTTGGAGTTGATGA+CGG | 0.536130 | 4.2:-2970144 | MS.gene06156:CDS |
| CGCCGTCATCAACTCCAACC+AGG | 0.570939 | 4.2:+2970142 | None:intergenic |
| CCATGGTTTCGACTAAGAGA+TGG | 0.572456 | 4.2:+2969765 | None:intergenic |
| GGTCATGAATGGTTGTATTG+TGG | 0.573456 | 4.2:-2969486 | MS.gene06156:CDS |
| CCTCGCCCGTAATGAAAACC+AGG | 0.578527 | 4.2:-2971092 | MS.gene06156:intron |
| GTTGCCAAAATGTCGATCCA+TGG | 0.581731 | 4.2:+2969748 | None:intergenic |
| GTGTTAATGGTGAACAAACA+AGG | 0.589164 | 4.2:-2971196 | MS.gene06156:CDS |
| TTGGAGGAAATGGTCATGAA+TGG | 0.600198 | 4.2:-2969497 | MS.gene06156:CDS |
| CCATCTCTTAGTCGAAACCA+TGG | 0.604583 | 4.2:-2969765 | MS.gene06156:CDS |
| ATTCATAATACTGAGCAAGA+CGG | 0.623435 | 4.2:+2971167 | None:intergenic |
| TTCGTCGTTCTTCAACAGTG+AGG | 0.697820 | 4.2:+2971143 | None:intergenic |
| GGTTGGAGTTGATGACGGCG+AGG | 0.701215 | 4.2:-2970139 | MS.gene06156:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAATAAAATCAATTTGTTT+TGG | + | chr4.2:2969873-2969892 | None:intergenic | 10.0% |
| !! | ATGAAAATAAAAGAAAAGAA+TGG | + | chr4.2:2969582-2969601 | None:intergenic | 15.0% |
| !! | ATATTTATCTACAATGAAAG+TGG | + | chr4.2:2970767-2970786 | None:intergenic | 20.0% |
| !! | ATTTAAACATGTGATGAAAT+TGG | + | chr4.2:2970148-2970167 | None:intergenic | 20.0% |
| !! | GCAACTACAAAATTATATAT+AGG | - | chr4.2:2970456-2970475 | MS.gene06156:intron | 20.0% |
| !! | TATCATGTTTCATTTAGAAA+AGG | - | chr4.2:2971110-2971129 | MS.gene06156:CDS | 20.0% |
| !! | TGAAATTGGAAAGAAAAAAA+AGG | + | chr4.2:2970134-2970153 | None:intergenic | 20.0% |
| !!! | ATTCTAGTTGATTTATACAA+AGG | - | chr4.2:2970549-2970568 | MS.gene06156:intron | 20.0% |
| !!! | TGAATATTTTTACTATAGAC+AGG | - | chr4.2:2970010-2970029 | MS.gene06156:intron | 20.0% |
| ! | AAACAATGACAATGTGAATT+AGG | + | chr4.2:2969637-2969656 | None:intergenic | 25.0% |
| ! | AGAAACAAAAAATCAGTGTT+TGG | + | chr4.2:2971048-2971067 | None:intergenic | 25.0% |
| ! | ATTAGGTAAAATTGAAGGTA+AGG | + | chr4.2:2969620-2969639 | None:intergenic | 25.0% |
| ! | GGAAGAAAACAATAGAATTA+TGG | + | chr4.2:2971000-2971019 | None:intergenic | 25.0% |
| ! | TAAATAAGACATAGATGTCT+TGG | - | chr4.2:2969975-2969994 | MS.gene06156:intron | 25.0% |
| ! | TAGATTATATAATCTGTGAG+TGG | - | chr4.2:2970193-2970212 | MS.gene06156:CDS | 25.0% |
| ! | TGTGAATTAGGTAAAATTGA+AGG | + | chr4.2:2969625-2969644 | None:intergenic | 25.0% |
| ! | TGTTTATTATTATTCAACCC+TGG | - | chr4.2:2969652-2969671 | MS.gene06156:intron | 25.0% |
| ! | TTGAATAATAAAGAATGACG+AGG | + | chr4.2:2970236-2970255 | None:intergenic | 25.0% |
| !!! | AGAAAAGGTACATTTTATGT+TGG | - | chr4.2:2971125-2971144 | MS.gene06156:CDS | 25.0% |
| !!! | CACTGTGTTATATTTTAGTT+GGG | - | chr4.2:2970106-2970125 | MS.gene06156:intron | 25.0% |
| AGAATTATGGAAGTAATGTG+AGG | + | chr4.2:2970987-2971006 | None:intergenic | 30.0% | |
| AGTAAGAGAAAGTCAAAAGA+TGG | + | chr4.2:2970311-2970330 | None:intergenic | 30.0% | |
| ATTCATAATACTGAGCAAGA+CGG | + | chr4.2:2969477-2969496 | None:intergenic | 30.0% | |
| ATTCTTTATTATTCAACCCG+AGG | - | chr4.2:2970240-2970259 | MS.gene06156:intron | 30.0% | |
| CCAACTAAAATATAACACAG+TGG | + | chr4.2:2970108-2970127 | None:intergenic | 30.0% | |
| GCATATTGTGTTAAATAGTC+TGG | - | chr4.2:2970592-2970611 | MS.gene06156:intron | 30.0% | |
| GGTTTATACCAGAAACATAA+GGG | - | chr4.2:2970345-2970364 | MS.gene06156:intron | 30.0% | |
| TATAGACAGGAGATAATTGT+TGG | - | chr4.2:2970023-2970042 | MS.gene06156:intron | 30.0% | |
| TGAGTGGAATGAAATTGAAA+TGG | - | chr4.2:2970209-2970228 | MS.gene06156:intron | 30.0% | |
| TGGTTTATACCAGAAACATA+AGG | - | chr4.2:2970344-2970363 | MS.gene06156:intron | 30.0% | |
| TTAACAAGACTAAGAACTTC+AGG | + | chr4.2:2970713-2970732 | None:intergenic | 30.0% | |
| TTATTATTATTCAACCCTGG+AGG | - | chr4.2:2969655-2969674 | MS.gene06156:intron | 30.0% | |
| TTTCATAATCTATCATGGAG+AGG | + | chr4.2:2969827-2969846 | None:intergenic | 30.0% | |
| ! | ACATTTTATGTTGGAGGAAA+TGG | - | chr4.2:2971134-2971153 | MS.gene06156:CDS | 30.0% |
| ! | CATAAACACTTCTGAGAATA+TGG | - | chr4.2:2969753-2969772 | MS.gene06156:CDS | 30.0% |
| ! | CTAGTTGATTTATACAAAGG+CGG | - | chr4.2:2970552-2970571 | MS.gene06156:intron | 30.0% |
| ! | GCTGATTTCATAATCTATCA+TGG | + | chr4.2:2969832-2969851 | None:intergenic | 30.0% |
| !! | AAAGGTACATTTTATGTTGG+AGG | - | chr4.2:2971128-2971147 | MS.gene06156:CDS | 30.0% |
| !! | AACATACCTGGTTTTCATTA+CGG | + | chr4.2:2969558-2969577 | None:intergenic | 30.0% |
| !!! | CCACTGTGTTATATTTTAGT+TGG | - | chr4.2:2970105-2970124 | MS.gene06156:intron | 30.0% |
| AAGTCAATCCCTTATGTTTC+TGG | + | chr4.2:2970356-2970375 | None:intergenic | 35.0% | |
| AATCTATCATGGAGAGGTTA+TGG | + | chr4.2:2969821-2969840 | None:intergenic | 35.0% | |
| AGCAAACTTGAATCCAAAGA+TGG | + | chr4.2:2970273-2970292 | None:intergenic | 35.0% | |
| AGGTAAAATTGAAGGTAAGG+AGG | + | chr4.2:2969617-2969636 | None:intergenic | 35.0% | |
| GAAAAGAATGGAAACATACC+TGG | + | chr4.2:2969570-2969589 | None:intergenic | 35.0% | |
| GTGTTAATGGTGAACAAACA+AGG | - | chr4.2:2969445-2969464 | MS.gene06156:CDS | 35.0% | |
| TCTACAATGAAAGTGGTACA+TGG | + | chr4.2:2970760-2970779 | None:intergenic | 35.0% | |
| TCTTACTCATATTGATGCGT+TGG | - | chr4.2:2970324-2970343 | MS.gene06156:intron | 35.0% | |
| TGTTCACCATTAACACGAAT+CGG | + | chr4.2:2969441-2969460 | None:intergenic | 35.0% | |
| TTCATAATACTGAGCAAGAC+GGG | + | chr4.2:2969476-2969495 | None:intergenic | 35.0% | |
| TTGAAGCTAAGTTCAGAAGA+TGG | + | chr4.2:2971025-2971044 | None:intergenic | 35.0% | |
| ! | ATTATTCAACCCGAGGTTTT+AGG | - | chr4.2:2970247-2970266 | MS.gene06156:intron | 35.0% |
| ! | GTTTTATCCATCAGCTGAAT+AGG | + | chr4.2:2971207-2971226 | None:intergenic | 35.0% |
| ! | TGGCTGATACATTTTTGTCA+TGG | - | chr4.2:2970410-2970429 | MS.gene06156:intron | 35.0% |
| !! | ACATACCTGGTTTTCATTAC+GGG | + | chr4.2:2969557-2969576 | None:intergenic | 35.0% |
| !!! | CTATGCATCTTTGTTTTTCC+TGG | - | chr4.2:2970481-2970500 | MS.gene06156:intron | 35.0% |
| AAAAGATGGCCTAAAACCTC+GGG | + | chr4.2:2970297-2970316 | None:intergenic | 40.0% | |
| ACGAACAATTTCGCCTTCAA+GGG | + | chr4.2:2969524-2969543 | None:intergenic | 40.0% | |
| ACTTTCTCTTCCCCAATTCT+GGG | + | chr4.2:2970061-2970080 | None:intergenic | 40.0% | |
| AGCTAAGTTCAGAAGATGGA+AGG | + | chr4.2:2971021-2971040 | None:intergenic | 40.0% | |
| AGTGGTCATCACTATGTGAT+CGG | - | chr4.2:2970632-2970651 | MS.gene06156:intron | 40.0% | |
| CAAAAGATGGCCTAAAACCT+CGG | + | chr4.2:2970298-2970317 | None:intergenic | 40.0% | |
| CAATCTAATTGCTACCTCCA+GGG | + | chr4.2:2969672-2969691 | None:intergenic | 40.0% | |
| CCAGTTTCTTTAGACAAGCA+AGG | + | chr4.2:2970663-2970682 | None:intergenic | 40.0% | |
| CCTTGCTTGTCTAAAGAAAC+TGG | - | chr4.2:2970660-2970679 | MS.gene06156:intron | 40.0% | |
| GGTCATGAATGGTTGTATTG+TGG | - | chr4.2:2971155-2971174 | MS.gene06156:CDS | 40.0% | |
| TACGAACAATTTCGCCTTCA+AGG | + | chr4.2:2969525-2969544 | None:intergenic | 40.0% | |
| TACTTTCTCTTCCCCAATTC+TGG | + | chr4.2:2970062-2970081 | None:intergenic | 40.0% | |
| TCAATCTAATTGCTACCTCC+AGG | + | chr4.2:2969673-2969692 | None:intergenic | 40.0% | |
| TCTGCAAAATTGTCGACAGA+AGG | + | chr4.2:2970831-2970850 | None:intergenic | 40.0% | |
| TGTATCAGCCAGATGAATCA+TGG | + | chr4.2:2970401-2970420 | None:intergenic | 40.0% | |
| TTCCTTTCACACCCAGAATT+GGG | - | chr4.2:2970047-2970066 | MS.gene06156:intron | 40.0% | |
| TTGGAGGAAATGGTCATGAA+TGG | - | chr4.2:2971144-2971163 | MS.gene06156:CDS | 40.0% | |
| TTTCCTTTCACACCCAGAAT+TGG | - | chr4.2:2970046-2970065 | MS.gene06156:intron | 40.0% | |
| ! | GAAACCATGGATCGACATTT+TGG | - | chr4.2:2970889-2970908 | MS.gene06156:intron | 40.0% |
| !!! | GCAGAATGAGCTTGCTATTT+TGG | - | chr4.2:2970846-2970865 | MS.gene06156:intron | 40.0% |
| !!! | GCATCTTTGTTTTTCCTGGT+TGG | - | chr4.2:2970485-2970504 | MS.gene06156:intron | 40.0% |
| CAAAGATGGCCTAAAACCTC+GGG | + | chr4.2:2970259-2970278 | None:intergenic | 45.0% | |
| CCAAAGATGGCCTAAAACCT+CGG | + | chr4.2:2970260-2970279 | None:intergenic | 45.0% | |
| CCATCTCTTAGTCGAAACCA+TGG | - | chr4.2:2970876-2970895 | MS.gene06156:intron | 45.0% | |
| CCATGGTTTCGACTAAGAGA+TGG | + | chr4.2:2970879-2970898 | None:intergenic | 45.0% | |
| GATCACATAGTGATGACCAC+TGG | + | chr4.2:2970633-2970652 | None:intergenic | 45.0% | |
| GCTTTGATCTTACTGACCAG+TGG | - | chr4.2:2970614-2970633 | MS.gene06156:intron | 45.0% | |
| GTTGCCAAAATGTCGATCCA+TGG | + | chr4.2:2970896-2970915 | None:intergenic | 45.0% | |
| TCCTTTCACACCCAGAATTG+GGG | - | chr4.2:2970048-2970067 | MS.gene06156:intron | 45.0% | |
| TCTGACTCCTATTCAGCTGA+TGG | - | chr4.2:2971197-2971216 | MS.gene06156:CDS | 45.0% | |
| TCTGCTCACCATGATTCATC+TGG | - | chr4.2:2970390-2970409 | MS.gene06156:intron | 45.0% | |
| TTCGTCGTTCTTCAACAGTG+AGG | + | chr4.2:2969501-2969520 | None:intergenic | 45.0% | |
| !! | TTCCTGGTTGGAGTTGATGA+CGG | - | chr4.2:2970497-2970516 | MS.gene06156:intron | 45.0% |
| !!! | AGTTTGCTACCCGAGGTTTT+AGG | - | chr4.2:2970285-2970304 | MS.gene06156:intron | 45.0% |
| ACAGAGACTCTAGTCACAGC+AGG | + | chr4.2:2970928-2970947 | None:intergenic | 50.0% | |
| GAAGAACGACGAACCCTTGA+AGG | - | chr4.2:2969508-2969527 | MS.gene06156:CDS | 50.0% | |
| TCCCCAATTCTGGGTGTGAA+AGG | + | chr4.2:2970052-2970071 | None:intergenic | 50.0% | |
| ! | GGGGATCCGATTCGTGTTAA+TGG | - | chr4.2:2969432-2969451 | MS.gene06156:CDS | 50.0% |
| !! | GGATCGACATTTTGGCAACG+TGG | - | chr4.2:2970897-2970916 | MS.gene06156:intron | 50.0% |
| !! | GGATTCAAGTTTGCTACCCG+AGG | - | chr4.2:2970278-2970297 | MS.gene06156:intron | 50.0% |
| !!! | CCGAGGTTTTAGGCCATCTT+TGG | - | chr4.2:2970257-2970276 | MS.gene06156:intron | 50.0% |
| CCTCGCCCGTAATGAAAACC+AGG | - | chr4.2:2969549-2969568 | MS.gene06156:intron | 55.0% | |
| !! | CCTGGTTTTCATTACGGGCG+AGG | + | chr4.2:2969552-2969571 | None:intergenic | 55.0% |
| CGCCGTCATCAACTCCAACC+AGG | + | chr4.2:2970502-2970521 | None:intergenic | 60.0% | |
| !! | GGTTGGAGTTGATGACGGCG+AGG | - | chr4.2:2970502-2970521 | MS.gene06156:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 2969430 | 2971233 | 2969430 | ID=MS.gene06156 |
| chr4.2 | mRNA | 2969430 | 2971233 | 2969430 | ID=MS.gene06156.t1;Parent=MS.gene06156 |
| chr4.2 | exon | 2971093 | 2971233 | 2971093 | ID=MS.gene06156.t1.exon1;Parent=MS.gene06156.t1 |
| chr4.2 | CDS | 2971093 | 2971233 | 2971093 | ID=cds.MS.gene06156.t1;Parent=MS.gene06156.t1 |
| chr4.2 | exon | 2970140 | 2970226 | 2970140 | ID=MS.gene06156.t1.exon2;Parent=MS.gene06156.t1 |
| chr4.2 | CDS | 2970140 | 2970226 | 2970140 | ID=cds.MS.gene06156.t1;Parent=MS.gene06156.t1 |
| chr4.2 | exon | 2969745 | 2969813 | 2969745 | ID=MS.gene06156.t1.exon3;Parent=MS.gene06156.t1 |
| chr4.2 | CDS | 2969745 | 2969813 | 2969745 | ID=cds.MS.gene06156.t1;Parent=MS.gene06156.t1 |
| chr4.2 | exon | 2969430 | 2969564 | 2969430 | ID=MS.gene06156.t1.exon4;Parent=MS.gene06156.t1 |
| chr4.2 | CDS | 2969430 | 2969564 | 2969430 | ID=cds.MS.gene06156.t1;Parent=MS.gene06156.t1 |
| Gene Sequence |
| Protein sequence |
