Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061582.t1 | XP_003600619.2 | 95 | 201 | 10 | 0 | 1 | 201 | 5 | 205 | 1.40E-82 | 315.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061582.t1 | I3S5Y6 | 95.0 | 201 | 10 | 0 | 1 | 201 | 5 | 205 | 1.0e-82 | 315.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049678 | MS.gene061582 | 0.978749 | 1.76E-146 | -1.69E-46 |
| MS.gene05031 | MS.gene061582 | 0.931806 | 2.19E-94 | -1.69E-46 |
| MS.gene050463 | MS.gene061582 | 0.841568 | 4.39E-58 | -1.69E-46 |
| MS.gene050528 | MS.gene061582 | 0.845969 | 2.92E-59 | -1.69E-46 |
| MS.gene051569 | MS.gene061582 | 0.851204 | 1.03E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061582.t1 | MTR_3g064350 | 98.010 | 201 | 4 | 0 | 1 | 201 | 5 | 205 | 3.17e-141 | 392 |
| MS.gene061582.t1 | MTR_6g090460 | 47.368 | 114 | 49 | 4 | 72 | 183 | 64 | 168 | 5.35e-22 | 88.6 |
| MS.gene061582.t1 | MTR_8g102350 | 55.556 | 63 | 26 | 2 | 71 | 131 | 75 | 137 | 5.13e-15 | 69.7 |
| MS.gene061582.t1 | MTR_3g105210 | 40.659 | 91 | 50 | 2 | 70 | 156 | 54 | 144 | 1.34e-12 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061582.t1 | AT5G56550 | 51.613 | 93 | 42 | 1 | 71 | 160 | 74 | 166 | 5.51e-18 | 77.8 |
| MS.gene061582.t1 | AT4G26288 | 56.061 | 66 | 21 | 2 | 71 | 132 | 59 | 120 | 1.78e-12 | 62.8 |
| MS.gene061582.t1 | AT5G21940 | 50.847 | 59 | 21 | 1 | 71 | 121 | 74 | 132 | 6.27e-11 | 60.5 |
Find 34 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGAAGTGGAAGTAACTTTA+TGG | 0.218762 | 3.2:+59881475 | MS.gene061582:CDS |
| TAGATTTAAAACTTGATTCT+TGG | 0.251748 | 3.2:-59881376 | None:intergenic |
| AAAGAAGACATATCACTTAA+TGG | 0.271135 | 3.2:-59881061 | None:intergenic |
| ATTAACAAATGTGAAGAGTT+TGG | 0.281541 | 3.2:+59881277 | MS.gene061582:CDS |
| CTCTTGTTGTAAGGACTCTC+TGG | 0.389294 | 3.2:-59881314 | None:intergenic |
| TTCTTCTTTGAATGCAAGAA+AGG | 0.409881 | 3.2:+59881454 | MS.gene061582:CDS |
| GACTTCAACCTCTTGTTGTA+AGG | 0.410223 | 3.2:-59881323 | None:intergenic |
| TCATCATAGTCTTCATGAAA+TGG | 0.421118 | 3.2:-59880887 | None:intergenic |
| GACTATGATGATATTATAGA+TGG | 0.427357 | 3.2:+59880899 | MS.gene061582:CDS |
| GATCTATTATGAGGATGAAT+TGG | 0.430717 | 3.2:-59881509 | None:intergenic |
| TCTGATTCTGAAGTTTCAAA+TGG | 0.436088 | 3.2:+59880935 | MS.gene061582:CDS |
| GGAAGTGGAAGTAACTTTAT+GGG | 0.451892 | 3.2:+59881476 | MS.gene061582:CDS |
| GGGTGAATTCAGCAAGTTCA+AGG | 0.456612 | 3.2:+59881423 | MS.gene061582:CDS |
| GAAGTAACTTTATGGGTAGT+AGG | 0.457592 | 3.2:+59881483 | MS.gene061582:CDS |
| TTGAAGTCATGTAGAAGCTA+TGG | 0.472853 | 3.2:+59881338 | MS.gene061582:CDS |
| TGTCAAGGCTTGTTTCTAAG+AGG | 0.473310 | 3.2:+59881399 | MS.gene061582:CDS |
| GAGGATGAAGAAGATGAAGT+AGG | 0.495656 | 3.2:-59881010 | None:intergenic |
| GTCAAGGCTTGTTTCTAAGA+GGG | 0.514801 | 3.2:+59881400 | MS.gene061582:CDS |
| AGTTTGATTAGAGATATTGT+TGG | 0.516221 | 3.2:-59881543 | None:intergenic |
| CTAGTAGATGATCTATTATG+AGG | 0.516955 | 3.2:-59881518 | None:intergenic |
| TTGAATGCAAGAAAGGGAAG+TGG | 0.520993 | 3.2:+59881461 | MS.gene061582:CDS |
| TCTTCTTTGAATGCAAGAAA+GGG | 0.526225 | 3.2:+59881455 | MS.gene061582:CDS |
| CAGAGAGTCCTTACAACAAG+AGG | 0.555710 | 3.2:+59881315 | MS.gene061582:CDS |
| CAAGGCTTGTTTCTAAGAGG+GGG | 0.561375 | 3.2:+59881402 | MS.gene061582:CDS |
| GGTGAATTCAGCAAGTTCAA+GGG | 0.566478 | 3.2:+59881424 | MS.gene061582:CDS |
| AAGTCATGTAGAAGCTATGG+AGG | 0.581269 | 3.2:+59881341 | MS.gene061582:CDS |
| AACAAATGTGAAGAGTTTGG+AGG | 0.582899 | 3.2:+59881280 | MS.gene061582:CDS |
| CTATTATGAGGATGAATTGG+TGG | 0.590564 | 3.2:-59881506 | None:intergenic |
| GTGAATTCAGCAAGTTCAAG+GGG | 0.594667 | 3.2:+59881425 | MS.gene061582:CDS |
| TCAAGGCTTGTTTCTAAGAG+GGG | 0.607183 | 3.2:+59881401 | MS.gene061582:CDS |
| GGACTTTCAAAATTCTACCA+AGG | 0.610945 | 3.2:+59881233 | MS.gene061582:CDS |
| AAGGCTTGTTTCTAAGAGGG+GGG | 0.642551 | 3.2:+59881403 | MS.gene061582:CDS |
| AGTCATGTAGAAGCTATGGA+GGG | 0.680331 | 3.2:+59881342 | MS.gene061582:CDS |
| TTCAAGTTGATGAGTAGATG+AGG | 0.689907 | 3.2:-59881029 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTATAGCTTTTTTTAA+TGG | + | chr3.2:59881125-59881144 | MS.gene061582:intron | 10.0% |
| !!! | TTTTTATAGCTTTTTTTAAT+GGG | + | chr3.2:59881126-59881145 | MS.gene061582:intron | 10.0% |
| !! | AAAAAAGCTATAAAAATTGA+TGG | - | chr3.2:59881122-59881141 | None:intergenic | 15.0% |
| !!! | TTCTTTTCATATTTGTTTTT+GGG | + | chr3.2:59881158-59881177 | MS.gene061582:intron | 15.0% |
| !!! | TTTCTTTTCATATTTGTTTT+TGG | + | chr3.2:59881157-59881176 | MS.gene061582:intron | 15.0% |
| !!! | TAGATTTAAAACTTGATTCT+TGG | - | chr3.2:59881379-59881398 | None:intergenic | 20.0% |
| ! | AAAGAAGACATATCACTTAA+TGG | - | chr3.2:59881064-59881083 | None:intergenic | 25.0% |
| ! | AGTTTGATTAGAGATATTGT+TGG | - | chr3.2:59881546-59881565 | None:intergenic | 25.0% |
| ! | ATTAACAAATGTGAAGAGTT+TGG | + | chr3.2:59881277-59881296 | MS.gene061582:CDS | 25.0% |
| ! | GACAAAAAAACTTACTTGAT+TGG | - | chr3.2:59881100-59881119 | None:intergenic | 25.0% |
| ! | GACTATGATGATATTATAGA+TGG | + | chr3.2:59880899-59880918 | MS.gene061582:CDS | 25.0% |
| !! | AAAGTTTGTGTCTTTTCTAT+AGG | + | chr3.2:59881207-59881226 | MS.gene061582:intron | 25.0% |
| !! | TAAAAGATTGTGATTTTCCT+TGG | - | chr3.2:59881253-59881272 | None:intergenic | 25.0% |
| !!! | CAAGTTTTAAATCTATGTCA+AGG | + | chr3.2:59881384-59881403 | MS.gene061582:CDS | 25.0% |
| CTAGTAGATGATCTATTATG+AGG | - | chr3.2:59881521-59881540 | None:intergenic | 30.0% | |
| GATCTATTATGAGGATGAAT+TGG | - | chr3.2:59881512-59881531 | None:intergenic | 30.0% | |
| TCATCATAGTCTTCATGAAA+TGG | - | chr3.2:59880890-59880909 | None:intergenic | 30.0% | |
| TCTTCTTTGAATGCAAGAAA+GGG | + | chr3.2:59881455-59881474 | MS.gene061582:CDS | 30.0% | |
| TTCTTCTTTGAATGCAAGAA+AGG | + | chr3.2:59881454-59881473 | MS.gene061582:CDS | 30.0% | |
| ! | TCATGAAATGGTCTTTTCTT+TGG | - | chr3.2:59880878-59880897 | None:intergenic | 30.0% |
| ! | TTTGTGTCTTTTCTATAGGA+GGG | + | chr3.2:59881211-59881230 | MS.gene061582:intron | 30.0% |
| !! | TCTGATTCTGAAGTTTCAAA+TGG | + | chr3.2:59880935-59880954 | MS.gene061582:CDS | 30.0% |
| AACAAATGTGAAGAGTTTGG+AGG | + | chr3.2:59881280-59881299 | MS.gene061582:CDS | 35.0% | |
| CTATTATGAGGATGAATTGG+TGG | - | chr3.2:59881509-59881528 | None:intergenic | 35.0% | |
| GAAGTAACTTTATGGGTAGT+AGG | + | chr3.2:59881483-59881502 | MS.gene061582:CDS | 35.0% | |
| GGAAGTGGAAGTAACTTTAT+GGG | + | chr3.2:59881476-59881495 | MS.gene061582:CDS | 35.0% | |
| GGACTTTCAAAATTCTACCA+AGG | + | chr3.2:59881233-59881252 | MS.gene061582:CDS | 35.0% | |
| TTGAAGTCATGTAGAAGCTA+TGG | + | chr3.2:59881338-59881357 | MS.gene061582:CDS | 35.0% | |
| ! | GTTTGTGTCTTTTCTATAGG+AGG | + | chr3.2:59881210-59881229 | MS.gene061582:intron | 35.0% |
| ! | TTGTGTCTTTTCTATAGGAG+GGG | + | chr3.2:59881212-59881231 | MS.gene061582:intron | 35.0% |
| !! | TTCAAGTTGATGAGTAGATG+AGG | - | chr3.2:59881032-59881051 | None:intergenic | 35.0% |
| AAGTCATGTAGAAGCTATGG+AGG | + | chr3.2:59881341-59881360 | MS.gene061582:CDS | 40.0% | |
| AGTCATGTAGAAGCTATGGA+GGG | + | chr3.2:59881342-59881361 | MS.gene061582:CDS | 40.0% | |
| GAGGATGAAGAAGATGAAGT+AGG | - | chr3.2:59881013-59881032 | None:intergenic | 40.0% | |
| GGGAAGTGGAAGTAACTTTA+TGG | + | chr3.2:59881475-59881494 | MS.gene061582:CDS | 40.0% | |
| GGTGAATTCAGCAAGTTCAA+GGG | + | chr3.2:59881424-59881443 | MS.gene061582:CDS | 40.0% | |
| GTGAATTCAGCAAGTTCAAG+GGG | + | chr3.2:59881425-59881444 | MS.gene061582:CDS | 40.0% | |
| TTGAATGCAAGAAAGGGAAG+TGG | + | chr3.2:59881461-59881480 | MS.gene061582:CDS | 40.0% | |
| ! | GACTTCAACCTCTTGTTGTA+AGG | - | chr3.2:59881326-59881345 | None:intergenic | 40.0% |
| !! | GTCAAGGCTTGTTTCTAAGA+GGG | + | chr3.2:59881400-59881419 | MS.gene061582:CDS | 40.0% |
| !! | TCAAGGCTTGTTTCTAAGAG+GGG | + | chr3.2:59881401-59881420 | MS.gene061582:CDS | 40.0% |
| !! | TGTCAAGGCTTGTTTCTAAG+AGG | + | chr3.2:59881399-59881418 | MS.gene061582:CDS | 40.0% |
| CAGAGAGTCCTTACAACAAG+AGG | + | chr3.2:59881315-59881334 | MS.gene061582:CDS | 45.0% | |
| GGGTGAATTCAGCAAGTTCA+AGG | + | chr3.2:59881423-59881442 | MS.gene061582:CDS | 45.0% | |
| ! | CTCTTGTTGTAAGGACTCTC+TGG | - | chr3.2:59881317-59881336 | None:intergenic | 45.0% |
| !! | AAGGCTTGTTTCTAAGAGGG+GGG | + | chr3.2:59881403-59881422 | MS.gene061582:CDS | 45.0% |
| !! | CAAGGCTTGTTTCTAAGAGG+GGG | + | chr3.2:59881402-59881421 | MS.gene061582:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 59880851 | 59881580 | 59880851 | ID=MS.gene061582 |
| chr3.2 | mRNA | 59880851 | 59881580 | 59880851 | ID=MS.gene061582.t1;Parent=MS.gene061582 |
| chr3.2 | exon | 59880851 | 59881104 | 59880851 | ID=MS.gene061582.t1.exon1;Parent=MS.gene061582.t1 |
| chr3.2 | CDS | 59880851 | 59881104 | 59880851 | ID=cds.MS.gene061582.t1;Parent=MS.gene061582.t1 |
| chr3.2 | exon | 59881229 | 59881580 | 59881229 | ID=MS.gene061582.t1.exon2;Parent=MS.gene061582.t1 |
| chr3.2 | CDS | 59881229 | 59881580 | 59881229 | ID=cds.MS.gene061582.t1;Parent=MS.gene061582.t1 |
| Gene Sequence |
| Protein sequence |
