Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049678.t1 | XP_003600619.2 | 95 | 201 | 10 | 0 | 1 | 201 | 5 | 205 | 3.10E-82 | 314.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049678.t1 | I3S5Y6 | 95.0 | 201 | 10 | 0 | 1 | 201 | 5 | 205 | 2.2e-82 | 314.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049678 | MS.gene05031 | 0.91408 | 2.92E-84 | -1.69E-46 |
| MS.gene049678 | MS.gene050463 | 0.833828 | 4.26E-56 | -1.69E-46 |
| MS.gene049678 | MS.gene050528 | 0.837303 | 5.63E-57 | -1.69E-46 |
| MS.gene049678 | MS.gene051569 | 0.831205 | 1.90E-55 | -1.69E-46 |
| MS.gene049678 | MS.gene061582 | 0.978749 | 1.76E-146 | -1.69E-46 |
| MS.gene049678 | MS.gene33033 | 0.829112 | 6.18E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049678.t1 | MTR_3g064350 | 98.010 | 201 | 4 | 0 | 1 | 201 | 5 | 205 | 9.40e-141 | 391 |
| MS.gene049678.t1 | MTR_6g090460 | 47.368 | 114 | 49 | 4 | 72 | 183 | 64 | 168 | 6.41e-22 | 88.2 |
| MS.gene049678.t1 | MTR_8g102350 | 55.556 | 63 | 26 | 2 | 71 | 131 | 75 | 137 | 5.69e-15 | 69.7 |
| MS.gene049678.t1 | MTR_3g105210 | 40.659 | 91 | 50 | 2 | 70 | 156 | 54 | 144 | 1.52e-12 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049678.t1 | AT5G56550 | 51.613 | 93 | 42 | 1 | 71 | 160 | 74 | 166 | 6.95e-18 | 77.8 |
| MS.gene049678.t1 | AT4G26288 | 56.061 | 66 | 21 | 2 | 71 | 132 | 59 | 120 | 1.99e-12 | 62.8 |
| MS.gene049678.t1 | AT5G21940 | 50.847 | 59 | 21 | 1 | 71 | 121 | 74 | 132 | 7.12e-11 | 60.1 |
Find 35 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGAAGTGGAAGTAACTTTA+TGG | 0.218762 | 3.3:+57903859 | MS.gene049678:CDS |
| TAGATTTAAAACTTGATTCT+TGG | 0.251748 | 3.3:-57903760 | None:intergenic |
| AAAGAAGACATATCACTTAA+TGG | 0.271135 | 3.3:-57903445 | None:intergenic |
| ATTAACAAATGTGAAGAGTT+TGG | 0.281541 | 3.3:+57903661 | MS.gene049678:CDS |
| TTCTTTGGTGATTCAATCAT+AGG | 0.303697 | 3.3:-57903244 | None:intergenic |
| CTCTTGTTGTAAGGACTCTC+TGG | 0.389294 | 3.3:-57903698 | None:intergenic |
| TTCTTCTTTGAATGCAAGAA+AGG | 0.409881 | 3.3:+57903838 | MS.gene049678:CDS |
| GACTTCAACCTCTTGTTGTA+AGG | 0.410223 | 3.3:-57903707 | None:intergenic |
| TCATCATAGTCTTCATGAAA+TGG | 0.421118 | 3.3:-57903271 | None:intergenic |
| GACTATGATGATATTATAGA+TGG | 0.427357 | 3.3:+57903283 | MS.gene049678:CDS |
| GATCTATTATGAGGATGAAT+TGG | 0.430717 | 3.3:-57903893 | None:intergenic |
| TCTGATTCTGAAGTTTCAAA+TGG | 0.436088 | 3.3:+57903319 | MS.gene049678:CDS |
| GGAAGTGGAAGTAACTTTAT+GGG | 0.451892 | 3.3:+57903860 | MS.gene049678:CDS |
| GGGTGAATTCAGCAAGTTCA+AGG | 0.456612 | 3.3:+57903807 | MS.gene049678:CDS |
| GAAGTAACTTTATGGGTAGT+AGG | 0.457592 | 3.3:+57903867 | MS.gene049678:CDS |
| TTGAAGTCATGTAGAAGCTA+TGG | 0.472853 | 3.3:+57903722 | MS.gene049678:CDS |
| TGTCAAGGCTTGTTTCTAAG+AGG | 0.473310 | 3.3:+57903783 | MS.gene049678:CDS |
| GAGGATGAAGAAGATGAAGT+AGG | 0.495656 | 3.3:-57903394 | None:intergenic |
| GTCAAGGCTTGTTTCTAAGA+GGG | 0.514801 | 3.3:+57903784 | MS.gene049678:CDS |
| AGTTTGATTAGAGATATTGT+TGG | 0.516221 | 3.3:-57903927 | None:intergenic |
| CTAGTAGATGATCTATTATG+AGG | 0.516955 | 3.3:-57903902 | None:intergenic |
| TTGAATGCAAGAAAGGGAAG+TGG | 0.520993 | 3.3:+57903845 | MS.gene049678:CDS |
| TCTTCTTTGAATGCAAGAAA+GGG | 0.526225 | 3.3:+57903839 | MS.gene049678:CDS |
| CAGAGAGTCCTTACAACAAG+AGG | 0.555710 | 3.3:+57903699 | MS.gene049678:CDS |
| CAAGGCTTGTTTCTAAGAGG+GGG | 0.561375 | 3.3:+57903786 | MS.gene049678:CDS |
| GGTGAATTCAGCAAGTTCAA+GGG | 0.566478 | 3.3:+57903808 | MS.gene049678:CDS |
| AAGTCATGTAGAAGCTATGG+AGG | 0.581269 | 3.3:+57903725 | MS.gene049678:CDS |
| AACAAATGTGAAGAGTTTGG+AGG | 0.582899 | 3.3:+57903664 | MS.gene049678:CDS |
| CTATTATGAGGATGAATTGG+TGG | 0.590564 | 3.3:-57903890 | None:intergenic |
| GTGAATTCAGCAAGTTCAAG+GGG | 0.594667 | 3.3:+57903809 | MS.gene049678:CDS |
| TCAAGGCTTGTTTCTAAGAG+GGG | 0.607183 | 3.3:+57903785 | MS.gene049678:CDS |
| GGACTTTCAAAATTCTACCA+AGG | 0.610945 | 3.3:+57903617 | MS.gene049678:CDS |
| AAGGCTTGTTTCTAAGAGGG+GGG | 0.642551 | 3.3:+57903787 | MS.gene049678:CDS |
| AGTCATGTAGAAGCTATGGA+GGG | 0.680331 | 3.3:+57903726 | MS.gene049678:CDS |
| TTCAAGTTGATGAGTAGATG+AGG | 0.689907 | 3.3:-57903413 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTATAGCTTTTTTTAA+TGG | + | chr3.3:57903509-57903528 | MS.gene049678:intron | 10.0% |
| !!! | TTTTTATAGCTTTTTTTAAT+GGG | + | chr3.3:57903510-57903529 | MS.gene049678:intron | 10.0% |
| !! | AAAAAAGCTATAAAAATTGA+TGG | - | chr3.3:57903506-57903525 | None:intergenic | 15.0% |
| !!! | TTCTTTTCATATTTGTTTTT+GGG | + | chr3.3:57903542-57903561 | MS.gene049678:intron | 15.0% |
| !!! | TTTCTTTTCATATTTGTTTT+TGG | + | chr3.3:57903541-57903560 | MS.gene049678:intron | 15.0% |
| !!! | TAGATTTAAAACTTGATTCT+TGG | - | chr3.3:57903763-57903782 | None:intergenic | 20.0% |
| ! | AAAGAAGACATATCACTTAA+TGG | - | chr3.3:57903448-57903467 | None:intergenic | 25.0% |
| ! | AGTTTGATTAGAGATATTGT+TGG | - | chr3.3:57903930-57903949 | None:intergenic | 25.0% |
| ! | ATTAACAAATGTGAAGAGTT+TGG | + | chr3.3:57903661-57903680 | MS.gene049678:CDS | 25.0% |
| ! | GACAAAAAAACTTACTTGAT+TGG | - | chr3.3:57903484-57903503 | None:intergenic | 25.0% |
| ! | GACTATGATGATATTATAGA+TGG | + | chr3.3:57903283-57903302 | MS.gene049678:CDS | 25.0% |
| !! | AAAGTTTGTGTCTTTTCTAT+AGG | + | chr3.3:57903591-57903610 | MS.gene049678:intron | 25.0% |
| !! | TAAAAGATTGTGATTTTCCT+TGG | - | chr3.3:57903637-57903656 | None:intergenic | 25.0% |
| !!! | CAAGTTTTAAATCTATGTCA+AGG | + | chr3.3:57903768-57903787 | MS.gene049678:CDS | 25.0% |
| CTAGTAGATGATCTATTATG+AGG | - | chr3.3:57903905-57903924 | None:intergenic | 30.0% | |
| GATCTATTATGAGGATGAAT+TGG | - | chr3.3:57903896-57903915 | None:intergenic | 30.0% | |
| TCATCATAGTCTTCATGAAA+TGG | - | chr3.3:57903274-57903293 | None:intergenic | 30.0% | |
| TCTTCTTTGAATGCAAGAAA+GGG | + | chr3.3:57903839-57903858 | MS.gene049678:CDS | 30.0% | |
| TTCTTCTTTGAATGCAAGAA+AGG | + | chr3.3:57903838-57903857 | MS.gene049678:CDS | 30.0% | |
| ! | TCATGAAATGGTCTTTTCTT+TGG | - | chr3.3:57903262-57903281 | None:intergenic | 30.0% |
| ! | TTCTTTGGTGATTCAATCAT+AGG | - | chr3.3:57903247-57903266 | None:intergenic | 30.0% |
| ! | TTTGTGTCTTTTCTATAGGA+GGG | + | chr3.3:57903595-57903614 | MS.gene049678:intron | 30.0% |
| !! | TCTGATTCTGAAGTTTCAAA+TGG | + | chr3.3:57903319-57903338 | MS.gene049678:CDS | 30.0% |
| AACAAATGTGAAGAGTTTGG+AGG | + | chr3.3:57903664-57903683 | MS.gene049678:CDS | 35.0% | |
| CTATTATGAGGATGAATTGG+TGG | - | chr3.3:57903893-57903912 | None:intergenic | 35.0% | |
| GAAGTAACTTTATGGGTAGT+AGG | + | chr3.3:57903867-57903886 | MS.gene049678:CDS | 35.0% | |
| GGAAGTGGAAGTAACTTTAT+GGG | + | chr3.3:57903860-57903879 | MS.gene049678:CDS | 35.0% | |
| GGACTTTCAAAATTCTACCA+AGG | + | chr3.3:57903617-57903636 | MS.gene049678:CDS | 35.0% | |
| TTGAAGTCATGTAGAAGCTA+TGG | + | chr3.3:57903722-57903741 | MS.gene049678:CDS | 35.0% | |
| ! | GTTTGTGTCTTTTCTATAGG+AGG | + | chr3.3:57903594-57903613 | MS.gene049678:intron | 35.0% |
| ! | TTGTGTCTTTTCTATAGGAG+GGG | + | chr3.3:57903596-57903615 | MS.gene049678:intron | 35.0% |
| !! | TTCAAGTTGATGAGTAGATG+AGG | - | chr3.3:57903416-57903435 | None:intergenic | 35.0% |
| AAGTCATGTAGAAGCTATGG+AGG | + | chr3.3:57903725-57903744 | MS.gene049678:CDS | 40.0% | |
| AGTCATGTAGAAGCTATGGA+GGG | + | chr3.3:57903726-57903745 | MS.gene049678:CDS | 40.0% | |
| GAGGATGAAGAAGATGAAGT+AGG | - | chr3.3:57903397-57903416 | None:intergenic | 40.0% | |
| GGGAAGTGGAAGTAACTTTA+TGG | + | chr3.3:57903859-57903878 | MS.gene049678:CDS | 40.0% | |
| GGTGAATTCAGCAAGTTCAA+GGG | + | chr3.3:57903808-57903827 | MS.gene049678:CDS | 40.0% | |
| GTGAATTCAGCAAGTTCAAG+GGG | + | chr3.3:57903809-57903828 | MS.gene049678:CDS | 40.0% | |
| TTGAATGCAAGAAAGGGAAG+TGG | + | chr3.3:57903845-57903864 | MS.gene049678:CDS | 40.0% | |
| ! | GACTTCAACCTCTTGTTGTA+AGG | - | chr3.3:57903710-57903729 | None:intergenic | 40.0% |
| !! | GTCAAGGCTTGTTTCTAAGA+GGG | + | chr3.3:57903784-57903803 | MS.gene049678:CDS | 40.0% |
| !! | TCAAGGCTTGTTTCTAAGAG+GGG | + | chr3.3:57903785-57903804 | MS.gene049678:CDS | 40.0% |
| !! | TGTCAAGGCTTGTTTCTAAG+AGG | + | chr3.3:57903783-57903802 | MS.gene049678:CDS | 40.0% |
| CAGAGAGTCCTTACAACAAG+AGG | + | chr3.3:57903699-57903718 | MS.gene049678:CDS | 45.0% | |
| GGGTGAATTCAGCAAGTTCA+AGG | + | chr3.3:57903807-57903826 | MS.gene049678:CDS | 45.0% | |
| ! | CTCTTGTTGTAAGGACTCTC+TGG | - | chr3.3:57903701-57903720 | None:intergenic | 45.0% |
| !! | AAGGCTTGTTTCTAAGAGGG+GGG | + | chr3.3:57903787-57903806 | MS.gene049678:CDS | 45.0% |
| !! | CAAGGCTTGTTTCTAAGAGG+GGG | + | chr3.3:57903786-57903805 | MS.gene049678:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 57903235 | 57903964 | 57903235 | ID=MS.gene049678 |
| chr3.3 | mRNA | 57903235 | 57903964 | 57903235 | ID=MS.gene049678.t1;Parent=MS.gene049678 |
| chr3.3 | exon | 57903235 | 57903488 | 57903235 | ID=MS.gene049678.t1.exon1;Parent=MS.gene049678.t1 |
| chr3.3 | CDS | 57903235 | 57903488 | 57903235 | ID=cds.MS.gene049678.t1;Parent=MS.gene049678.t1 |
| chr3.3 | exon | 57903613 | 57903964 | 57903613 | ID=MS.gene049678.t1.exon2;Parent=MS.gene049678.t1 |
| chr3.3 | CDS | 57903613 | 57903964 | 57903613 | ID=cds.MS.gene049678.t1;Parent=MS.gene049678.t1 |
| Gene Sequence |
| Protein sequence |
