Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05031.t1 | XP_003600619.2 | 95.1 | 205 | 10 | 0 | 1 | 205 | 1 | 205 | 1.50E-84 | 322.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05031.t1 | I3S5Y6 | 95.1 | 205 | 10 | 0 | 1 | 205 | 1 | 205 | 1.1e-84 | 322.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049678 | MS.gene05031 | 0.91408 | 2.92E-84 | -1.69E-46 |
| MS.gene05031 | MS.gene050463 | 0.843014 | 1.82E-58 | -1.69E-46 |
| MS.gene05031 | MS.gene050528 | 0.84291 | 1.94E-58 | -1.69E-46 |
| MS.gene05031 | MS.gene051569 | 0.843961 | 1.02E-58 | -1.69E-46 |
| MS.gene05031 | MS.gene061582 | 0.931806 | 2.19E-94 | -1.69E-46 |
| MS.gene05031 | MS.gene33033 | 0.84667 | 1.88E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05031.t1 | MTR_3g064350 | 98.049 | 205 | 4 | 0 | 1 | 205 | 1 | 205 | 8.83e-145 | 401 |
| MS.gene05031.t1 | MTR_6g090460 | 47.368 | 114 | 49 | 4 | 76 | 187 | 64 | 168 | 5.29e-22 | 88.6 |
| MS.gene05031.t1 | MTR_8g102350 | 55.556 | 63 | 26 | 2 | 75 | 135 | 75 | 137 | 5.53e-15 | 69.7 |
| MS.gene05031.t1 | MTR_3g105210 | 40.659 | 91 | 50 | 2 | 74 | 160 | 54 | 144 | 1.55e-12 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05031.t1 | AT5G56550 | 51.613 | 93 | 42 | 1 | 75 | 164 | 74 | 166 | 5.24e-18 | 78.2 |
| MS.gene05031.t1 | AT4G26288 | 56.061 | 66 | 21 | 2 | 75 | 136 | 59 | 120 | 1.80e-12 | 63.2 |
| MS.gene05031.t1 | AT5G21940 | 50.847 | 59 | 21 | 1 | 75 | 125 | 74 | 132 | 7.38e-11 | 60.5 |
Find 36 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGAAGTGGAAGTAACTTTA+TGG | 0.218762 | 3.4:+66092113 | MS.gene05031:CDS |
| TAGATTTAAAACTTGATTCT+TGG | 0.251748 | 3.4:-66092014 | None:intergenic |
| ATTAACAAATGTGAAGAGTT+TGG | 0.281541 | 3.4:+66091915 | MS.gene05031:CDS |
| AGAGAAGACATATCACTTAA+TGG | 0.324873 | 3.4:-66091699 | None:intergenic |
| AGGATGAAGAAGATGAAGTA+GGG | 0.381879 | 3.4:-66091647 | None:intergenic |
| CTCTTGTTGTAAGGACTCTC+TGG | 0.389294 | 3.4:-66091952 | None:intergenic |
| TTCTTCTTTGAATGCAAGAA+AGG | 0.409881 | 3.4:+66092092 | MS.gene05031:CDS |
| GACTTCAACCTCTTGTTGTA+AGG | 0.410223 | 3.4:-66091961 | None:intergenic |
| TCATCATAGTCTTCATGAAA+TGG | 0.421118 | 3.4:-66091525 | None:intergenic |
| GAGGATGAAGAAGATGAAGT+AGG | 0.424863 | 3.4:-66091648 | None:intergenic |
| GACTATGATGATATTATAGA+TGG | 0.427357 | 3.4:+66091537 | MS.gene05031:CDS |
| GATCTATTATGAGGATGAAT+TGG | 0.430717 | 3.4:-66092147 | None:intergenic |
| TCTGATTCTGAAGTTTCAAA+TGG | 0.436088 | 3.4:+66091573 | MS.gene05031:CDS |
| GGAAGTGGAAGTAACTTTAT+GGG | 0.451892 | 3.4:+66092114 | MS.gene05031:CDS |
| GGGTGAATTCAGCAAGTTCA+AGG | 0.456612 | 3.4:+66092061 | MS.gene05031:CDS |
| GAAGTAACTTTATGGGTAGT+AGG | 0.457592 | 3.4:+66092121 | MS.gene05031:CDS |
| TTGAAGTCATGTAGAAGCTA+TGG | 0.472853 | 3.4:+66091976 | MS.gene05031:CDS |
| TGTCAAGGCTTGTTTCTAAG+AGG | 0.473310 | 3.4:+66092037 | MS.gene05031:CDS |
| GTCAAGGCTTGTTTCTAAGA+GGG | 0.514801 | 3.4:+66092038 | MS.gene05031:CDS |
| AGTTTGATTAGAGATATTGT+TGG | 0.516221 | 3.4:-66092181 | None:intergenic |
| CTAGTAGATGATCTATTATG+AGG | 0.516955 | 3.4:-66092156 | None:intergenic |
| TTGAATGCAAGAAAGGGAAG+TGG | 0.520993 | 3.4:+66092099 | MS.gene05031:CDS |
| TCTTCTTTGAATGCAAGAAA+GGG | 0.526225 | 3.4:+66092093 | MS.gene05031:CDS |
| CAGAGAGTCCTTACAACAAG+AGG | 0.555710 | 3.4:+66091953 | MS.gene05031:CDS |
| CAAGGCTTGTTTCTAAGAGG+GGG | 0.561375 | 3.4:+66092040 | MS.gene05031:CDS |
| GGATGAAGAAGATGAAGTAG+GGG | 0.561740 | 3.4:-66091646 | None:intergenic |
| GGTGAATTCAGCAAGTTCAA+GGG | 0.566478 | 3.4:+66092062 | MS.gene05031:CDS |
| AAGTCATGTAGAAGCTATGG+AGG | 0.581269 | 3.4:+66091979 | MS.gene05031:CDS |
| AACAAATGTGAAGAGTTTGG+AGG | 0.582899 | 3.4:+66091918 | MS.gene05031:CDS |
| CTATTATGAGGATGAATTGG+TGG | 0.590564 | 3.4:-66092144 | None:intergenic |
| GTGAATTCAGCAAGTTCAAG+GGG | 0.594667 | 3.4:+66092063 | MS.gene05031:CDS |
| TCAAGGCTTGTTTCTAAGAG+GGG | 0.607183 | 3.4:+66092039 | MS.gene05031:CDS |
| GGACTTTCAAAATTCTACCA+AGG | 0.610945 | 3.4:+66091871 | MS.gene05031:CDS |
| AAGGCTTGTTTCTAAGAGGG+GGG | 0.642551 | 3.4:+66092041 | MS.gene05031:CDS |
| AGTCATGTAGAAGCTATGGA+GGG | 0.680331 | 3.4:+66091980 | MS.gene05031:CDS |
| TTCAAGTTGATGAGTAGATG+AGG | 0.689907 | 3.4:-66091667 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTATAGCTTTTTTTAA+TGG | + | chr3.4:66091763-66091782 | MS.gene05031:intron | 10.0% |
| !!! | TTTTTATAGCTTTTTTTAAT+GGG | + | chr3.4:66091764-66091783 | MS.gene05031:intron | 10.0% |
| !! | AAAAAAGCTATAAAAATTGA+TGG | - | chr3.4:66091760-66091779 | None:intergenic | 15.0% |
| !!! | TTCTTTTCATATTTGTTTTT+GGG | + | chr3.4:66091796-66091815 | MS.gene05031:intron | 15.0% |
| !!! | TTTCTTTTCATATTTGTTTT+TGG | + | chr3.4:66091795-66091814 | MS.gene05031:intron | 15.0% |
| !!! | TAGATTTAAAACTTGATTCT+TGG | - | chr3.4:66092017-66092036 | None:intergenic | 20.0% |
| ! | AGTTTGATTAGAGATATTGT+TGG | - | chr3.4:66092184-66092203 | None:intergenic | 25.0% |
| ! | ATTAACAAATGTGAAGAGTT+TGG | + | chr3.4:66091915-66091934 | MS.gene05031:CDS | 25.0% |
| ! | GACAAAAAAACTTACTTGAT+TGG | - | chr3.4:66091738-66091757 | None:intergenic | 25.0% |
| ! | GACTATGATGATATTATAGA+TGG | + | chr3.4:66091537-66091556 | MS.gene05031:CDS | 25.0% |
| !! | AAAGTTTGTGTCTTTTCTAT+AGG | + | chr3.4:66091845-66091864 | MS.gene05031:intron | 25.0% |
| !! | TAAAAGATTGTGATTTTCCT+TGG | - | chr3.4:66091891-66091910 | None:intergenic | 25.0% |
| !!! | CAAGTTTTAAATCTATGTCA+AGG | + | chr3.4:66092022-66092041 | MS.gene05031:CDS | 25.0% |
| AGAGAAGACATATCACTTAA+TGG | - | chr3.4:66091702-66091721 | None:intergenic | 30.0% | |
| CTAGTAGATGATCTATTATG+AGG | - | chr3.4:66092159-66092178 | None:intergenic | 30.0% | |
| GATCTATTATGAGGATGAAT+TGG | - | chr3.4:66092150-66092169 | None:intergenic | 30.0% | |
| TCATCATAGTCTTCATGAAA+TGG | - | chr3.4:66091528-66091547 | None:intergenic | 30.0% | |
| TCTTCTTTGAATGCAAGAAA+GGG | + | chr3.4:66092093-66092112 | MS.gene05031:CDS | 30.0% | |
| TTCTTCTTTGAATGCAAGAA+AGG | + | chr3.4:66092092-66092111 | MS.gene05031:CDS | 30.0% | |
| ! | TCATGAAATGGTCTTTTCTT+TGG | - | chr3.4:66091516-66091535 | None:intergenic | 30.0% |
| ! | TTTGTGTCTTTTCTATAGGA+GGG | + | chr3.4:66091849-66091868 | MS.gene05031:intron | 30.0% |
| !! | TCTGATTCTGAAGTTTCAAA+TGG | + | chr3.4:66091573-66091592 | MS.gene05031:CDS | 30.0% |
| AACAAATGTGAAGAGTTTGG+AGG | + | chr3.4:66091918-66091937 | MS.gene05031:CDS | 35.0% | |
| AGGATGAAGAAGATGAAGTA+GGG | - | chr3.4:66091650-66091669 | None:intergenic | 35.0% | |
| CTATTATGAGGATGAATTGG+TGG | - | chr3.4:66092147-66092166 | None:intergenic | 35.0% | |
| GAAGTAACTTTATGGGTAGT+AGG | + | chr3.4:66092121-66092140 | MS.gene05031:CDS | 35.0% | |
| GGAAGTGGAAGTAACTTTAT+GGG | + | chr3.4:66092114-66092133 | MS.gene05031:CDS | 35.0% | |
| GGACTTTCAAAATTCTACCA+AGG | + | chr3.4:66091871-66091890 | MS.gene05031:CDS | 35.0% | |
| TTGAAGTCATGTAGAAGCTA+TGG | + | chr3.4:66091976-66091995 | MS.gene05031:CDS | 35.0% | |
| ! | GTTTGTGTCTTTTCTATAGG+AGG | + | chr3.4:66091848-66091867 | MS.gene05031:intron | 35.0% |
| ! | TTGTGTCTTTTCTATAGGAG+GGG | + | chr3.4:66091850-66091869 | MS.gene05031:intron | 35.0% |
| !! | TTCAAGTTGATGAGTAGATG+AGG | - | chr3.4:66091670-66091689 | None:intergenic | 35.0% |
| AAGTCATGTAGAAGCTATGG+AGG | + | chr3.4:66091979-66091998 | MS.gene05031:CDS | 40.0% | |
| AGTCATGTAGAAGCTATGGA+GGG | + | chr3.4:66091980-66091999 | MS.gene05031:CDS | 40.0% | |
| GAGGATGAAGAAGATGAAGT+AGG | - | chr3.4:66091651-66091670 | None:intergenic | 40.0% | |
| GGATGAAGAAGATGAAGTAG+GGG | - | chr3.4:66091649-66091668 | None:intergenic | 40.0% | |
| GGGAAGTGGAAGTAACTTTA+TGG | + | chr3.4:66092113-66092132 | MS.gene05031:CDS | 40.0% | |
| GGTGAATTCAGCAAGTTCAA+GGG | + | chr3.4:66092062-66092081 | MS.gene05031:CDS | 40.0% | |
| GTGAATTCAGCAAGTTCAAG+GGG | + | chr3.4:66092063-66092082 | MS.gene05031:CDS | 40.0% | |
| TTGAATGCAAGAAAGGGAAG+TGG | + | chr3.4:66092099-66092118 | MS.gene05031:CDS | 40.0% | |
| ! | GACTTCAACCTCTTGTTGTA+AGG | - | chr3.4:66091964-66091983 | None:intergenic | 40.0% |
| !! | GTCAAGGCTTGTTTCTAAGA+GGG | + | chr3.4:66092038-66092057 | MS.gene05031:CDS | 40.0% |
| !! | TCAAGGCTTGTTTCTAAGAG+GGG | + | chr3.4:66092039-66092058 | MS.gene05031:CDS | 40.0% |
| !! | TGTCAAGGCTTGTTTCTAAG+AGG | + | chr3.4:66092037-66092056 | MS.gene05031:CDS | 40.0% |
| CAGAGAGTCCTTACAACAAG+AGG | + | chr3.4:66091953-66091972 | MS.gene05031:CDS | 45.0% | |
| GGGTGAATTCAGCAAGTTCA+AGG | + | chr3.4:66092061-66092080 | MS.gene05031:CDS | 45.0% | |
| ! | CTCTTGTTGTAAGGACTCTC+TGG | - | chr3.4:66091955-66091974 | None:intergenic | 45.0% |
| !! | AAGGCTTGTTTCTAAGAGGG+GGG | + | chr3.4:66092041-66092060 | MS.gene05031:CDS | 45.0% |
| !! | CAAGGCTTGTTTCTAAGAGG+GGG | + | chr3.4:66092040-66092059 | MS.gene05031:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 66091477 | 66092218 | 66091477 | ID=MS.gene05031 |
| chr3.4 | mRNA | 66091477 | 66092218 | 66091477 | ID=MS.gene05031.t1;Parent=MS.gene05031 |
| chr3.4 | exon | 66091477 | 66091742 | 66091477 | ID=MS.gene05031.t1.exon1;Parent=MS.gene05031.t1 |
| chr3.4 | CDS | 66091477 | 66091742 | 66091477 | ID=cds.MS.gene05031.t1;Parent=MS.gene05031.t1 |
| chr3.4 | exon | 66091867 | 66092218 | 66091867 | ID=MS.gene05031.t1.exon2;Parent=MS.gene05031.t1 |
| chr3.4 | CDS | 66091867 | 66092218 | 66091867 | ID=cds.MS.gene05031.t1;Parent=MS.gene05031.t1 |
| Gene Sequence |
| Protein sequence |
