Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061903.t1 | XP_003625515.1 | 100 | 143 | 0 | 0 | 1 | 143 | 1 | 143 | 4.90E-74 | 287 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061903.t1 | O82201 | 91.5 | 141 | 12 | 0 | 1 | 141 | 1 | 141 | 2.2e-70 | 266.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061903.t1 | G7L0L8 | 100.0 | 143 | 0 | 0 | 1 | 143 | 1 | 143 | 3.6e-74 | 287.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene061903 | MS.gene64216 | PPI |
| MS.gene061903 | MS.gene75781 | PPI |
| MS.gene061903 | MS.gene64013 | PPI |
| MS.gene061903 | MS.gene021005 | PPI |
| MS.gene061903 | MS.gene27073 | PPI |
| MS.gene061903 | MS.gene017498 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061903.t1 | MTR_7g100010 | 100.000 | 143 | 0 | 0 | 1 | 143 | 1 | 143 | 2.71e-104 | 294 |
| MS.gene061903.t1 | MTR_8g102180 | 100.000 | 143 | 0 | 0 | 1 | 143 | 1 | 143 | 2.71e-104 | 294 |
| MS.gene061903.t1 | MTR_5g098940 | 42.754 | 138 | 79 | 0 | 3 | 140 | 2 | 139 | 6.08e-39 | 129 |
| MS.gene061903.t1 | MTR_1g025190 | 46.763 | 139 | 74 | 0 | 3 | 141 | 2 | 140 | 2.03e-38 | 127 |
| MS.gene061903.t1 | MTR_5g087820 | 42.537 | 134 | 77 | 0 | 3 | 136 | 26 | 159 | 4.96e-35 | 120 |
| MS.gene061903.t1 | MTR_8g077600 | 37.838 | 148 | 79 | 3 | 3 | 141 | 2 | 145 | 7.50e-31 | 108 |
| MS.gene061903.t1 | MTR_8g077600 | 36.559 | 93 | 46 | 3 | 3 | 86 | 2 | 90 | 8.93e-12 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061903.t1 | AT2G19790 | 90.845 | 142 | 13 | 0 | 1 | 142 | 1 | 142 | 1.27e-96 | 275 |
| MS.gene061903.t1 | AT1G47830 | 47.482 | 139 | 73 | 0 | 3 | 141 | 2 | 140 | 3.80e-39 | 129 |
| MS.gene061903.t1 | AT2G17380 | 42.446 | 139 | 80 | 0 | 3 | 141 | 2 | 140 | 9.73e-39 | 129 |
| MS.gene061903.t1 | AT4G35410 | 42.446 | 139 | 80 | 0 | 3 | 141 | 2 | 140 | 1.94e-37 | 125 |
| MS.gene061903.t1 | AT3G50860 | 40.278 | 144 | 81 | 1 | 3 | 141 | 2 | 145 | 3.65e-35 | 120 |
| MS.gene061903.t1 | AT3G50860 | 40.278 | 144 | 81 | 1 | 3 | 141 | 2 | 145 | 3.72e-35 | 119 |
| MS.gene061903.t1 | AT4G35410 | 46.392 | 97 | 52 | 0 | 3 | 99 | 2 | 98 | 1.37e-25 | 94.0 |
Find 20 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAGAATGAGCTTGCTATTT+TGG | 0.125124 | 7.2:-12013607 | MS.gene061903:intron |
| GAAACCATGGATCGACATTT+TGG | 0.210891 | 7.2:-12013564 | MS.gene061903:CDS |
| TCTGACTCCTATTCAGCTTA+TGG | 0.236679 | 7.2:-12013257 | MS.gene061903:CDS |
| GGGGATCCGATTCGTGTTAA+TGG | 0.262612 | 7.2:-12014998 | MS.gene061903:CDS |
| TACGAACGATTTCGCCTTCA+AGG | 0.388788 | 7.2:+12014908 | None:intergenic |
| GAAGAACGACGAACCCTTGA+AGG | 0.434936 | 7.2:-12014922 | MS.gene061903:CDS |
| TGTTCACCATTAACACGAAT+CGG | 0.437301 | 7.2:+12014992 | None:intergenic |
| TATCATGTTTCATTTAGAAA+AGG | 0.473226 | 7.2:-12013344 | MS.gene061903:CDS |
| GCAACTACAAAATTGTATAC+AGG | 0.518035 | 7.2:-12014004 | MS.gene061903:CDS |
| ACGAACGATTTCGCCTTCAA+GGG | 0.529007 | 7.2:+12014909 | None:intergenic |
| TTTCTGGTTGGAGTTGATGA+CGG | 0.545713 | 7.2:-12013963 | MS.gene061903:CDS |
| GGTCATGAATGGTTGTATTG+TGG | 0.573456 | 7.2:-12013299 | MS.gene061903:CDS |
| CCTCGCCCGTAATGAAAACC+AGG | 0.578527 | 7.2:-12014881 | MS.gene061903:intron |
| CCATGGTTTCGACTAAGAGA+TGG | 0.580792 | 7.2:+12013577 | None:intergenic |
| GTTGCCAAAATGTCGATCCA+TGG | 0.581731 | 7.2:+12013560 | None:intergenic |
| GTGTTAATGGTGAACAAACA+AGG | 0.585214 | 7.2:-12014985 | MS.gene061903:CDS |
| TTGGAGGAAATGGTCATGAA+TGG | 0.600198 | 7.2:-12013310 | MS.gene061903:CDS |
| CCATCTCTTAGTCGAAACCA+TGG | 0.604583 | 7.2:-12013577 | MS.gene061903:CDS |
| GGTTGGAGTTGATGACGGCG+AGG | 0.687099 | 7.2:-12013958 | MS.gene061903:intron |
| TTCGTCGTTCTTCAACAGTG+AGG | 0.697820 | 7.2:+12014932 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTTAATAAAATAAAAAACTT+AGG | - | chr7.2:12013978-12013997 | MS.gene061903:CDS | 10.0% |
| !! | ATAAATAATAAACAATGACA+AGG | + | chr7.2:12013456-12013475 | None:intergenic | 15.0% |
| !! | ATGAAAATAAAAGAAAAGAA+TGG | + | chr7.2:12013395-12013414 | None:intergenic | 15.0% |
| !!! | TTGAAATTTTTATCATAGAT+GGG | - | chr7.2:12013845-12013864 | MS.gene061903:intron | 15.0% |
| !!! | TTTGAAATTTTTATCATAGA+TGG | - | chr7.2:12013844-12013863 | MS.gene061903:intron | 15.0% |
| !! | ACAATACTAATAATAACTTC+AGG | + | chr7.2:12014500-12014519 | None:intergenic | 20.0% |
| !! | ATTAGCTAAAATTGAATGTA+AGG | + | chr7.2:12013430-12013449 | None:intergenic | 20.0% |
| !! | CGATATCAATTTATTTACAA+TGG | + | chr7.2:12014563-12014582 | None:intergenic | 20.0% |
| !! | TAAATAAGATATAGATGTCT+TGG | - | chr7.2:12013811-12013830 | MS.gene061903:intron | 20.0% |
| !! | TATCATGTTTCATTTAGAAA+AGG | - | chr7.2:12014899-12014918 | MS.gene061903:CDS | 20.0% |
| !! | TCAAAATTGAAACAAAAACT+AGG | - | chr7.2:12013538-12013557 | MS.gene061903:intron | 20.0% |
| !! | TTTACAACAGAAAATTCATT+CGG | + | chr7.2:12014469-12014488 | None:intergenic | 20.0% |
| !!! | ATTCTAGTTGATTTATACAA+AGG | - | chr7.2:12014332-12014351 | MS.gene061903:intron | 20.0% |
| ! | AGAAACAAAAAATCAGTGTT+TGG | + | chr7.2:12014840-12014859 | None:intergenic | 25.0% |
| ! | GAAGAAAACAATAGACATTA+TGG | + | chr7.2:12014791-12014810 | None:intergenic | 25.0% |
| ! | GTTAGTTACCAAAAACATAT+AGG | - | chr7.2:12014134-12014153 | MS.gene061903:intron | 25.0% |
| !!! | AGAAAAGGTACATTTTATGT+TGG | - | chr7.2:12014914-12014933 | MS.gene061903:CDS | 25.0% |
| !!! | ATTTATTCAACCCTAGTTTT+AGG | - | chr7.2:12013470-12013489 | MS.gene061903:intron | 25.0% |
| !!! | TTTATTCAACCCTAGTTTTA+GGG | - | chr7.2:12013471-12013490 | MS.gene061903:intron | 25.0% |
| AATCATTGACCTAATACCTT+AGG | + | chr7.2:12014047-12014066 | None:intergenic | 30.0% | |
| AATCTATCATGGAGAACTTA+TGG | + | chr7.2:12013658-12013677 | None:intergenic | 30.0% | |
| AATTGATATCGCTTCAAATC+TGG | - | chr7.2:12014572-12014591 | MS.gene061903:intron | 30.0% | |
| ACAGTTGACAATAATAACAC+AGG | + | chr7.2:12013958-12013977 | None:intergenic | 30.0% | |
| AGTTGACAATAATAACACAG+GGG | + | chr7.2:12013956-12013975 | None:intergenic | 30.0% | |
| CAGTTGACAATAATAACACA+GGG | + | chr7.2:12013957-12013976 | None:intergenic | 30.0% | |
| CTAAAGCCAATTTCAGAATA+TGG | + | chr7.2:12014817-12014836 | None:intergenic | 30.0% | |
| GCAACTACAAAATTGTATAC+AGG | - | chr7.2:12014239-12014258 | MS.gene061903:intron | 30.0% | |
| TAAGATATAGATGTCTTGGA+AGG | - | chr7.2:12013815-12013834 | MS.gene061903:intron | 30.0% | |
| TATACAGGAATGAAAGAAAG+CGG | + | chr7.2:12014010-12014029 | None:intergenic | 30.0% | |
| TCCTGTATATCAATACAACA+TGG | - | chr7.2:12014021-12014040 | MS.gene061903:CDS | 30.0% | |
| TTACAATGGAAGTGATACAT+GGG | + | chr7.2:12014549-12014568 | None:intergenic | 30.0% | |
| TTTACAATGGAAGTGATACA+TGG | + | chr7.2:12014550-12014569 | None:intergenic | 30.0% | |
| ! | ACATTTTATGTTGGAGGAAA+TGG | - | chr7.2:12014923-12014942 | MS.gene061903:CDS | 30.0% |
| ! | CAACTGAACCTATATGTTTT+TGG | + | chr7.2:12014145-12014164 | None:intergenic | 30.0% |
| ! | CTAGTTGATTTATACAAAGG+TGG | - | chr7.2:12014335-12014354 | MS.gene061903:intron | 30.0% |
| ! | GCTGATTTCATAATCTATCA+TGG | + | chr7.2:12013669-12013688 | None:intergenic | 30.0% |
| ! | GTTTTATCCATAAGCTGAAT+AGG | + | chr7.2:12014996-12015015 | None:intergenic | 30.0% |
| !! | AAAGGTACATTTTATGTTGG+AGG | - | chr7.2:12014917-12014936 | MS.gene061903:CDS | 30.0% |
| !! | TTCCTTCCATATTCTGAAAT+TGG | - | chr7.2:12014808-12014827 | MS.gene061903:intron | 30.0% |
| !!! | CTATGCATCTTTGTTTTTTC+TGG | - | chr7.2:12014264-12014283 | MS.gene061903:intron | 30.0% |
| AGCCAATTTCAGAATATGGA+AGG | + | chr7.2:12014813-12014832 | None:intergenic | 35.0% | |
| ATTGTTCATTCTACCTTCTG+TGG | - | chr7.2:12014605-12014624 | MS.gene061903:intron | 35.0% | |
| GACATTATGGAAGTAATGTG+AGG | + | chr7.2:12014778-12014797 | None:intergenic | 35.0% | |
| GGAAAAGAAAGTAGTTAGTG+AGG | - | chr7.2:12013907-12013926 | MS.gene061903:intron | 35.0% | |
| GTGTTAATGGTGAACAAACA+AGG | - | chr7.2:12013258-12013277 | MS.gene061903:CDS | 35.0% | |
| TATCAATACAACATGGCCTA+AGG | - | chr7.2:12014028-12014047 | MS.gene061903:intron | 35.0% | |
| TCTGCAAAATTAACCACAGA+AGG | + | chr7.2:12014621-12014640 | None:intergenic | 35.0% | |
| TGTTCACCATTAACACGAAT+CGG | + | chr7.2:12013254-12013273 | None:intergenic | 35.0% | |
| ! | CTACTTTCTTTTCCCCAATT+TGG | + | chr7.2:12013901-12013920 | None:intergenic | 35.0% |
| ! | GCCATGTTGTATTGATATAC+AGG | + | chr7.2:12014025-12014044 | None:intergenic | 35.0% |
| ! | TGGCTGATACATTTTTGTCA+TGG | - | chr7.2:12014193-12014212 | MS.gene061903:intron | 35.0% |
| !! | AGCATACCTGGTTTTCATTA+CGG | + | chr7.2:12013371-12013390 | None:intergenic | 35.0% |
| !!! | GCATCTTTGTTTTTTCTGGT+TGG | - | chr7.2:12014268-12014287 | MS.gene061903:intron | 35.0% |
| ATTACGCTGAAGCTGAAATG+TGG | + | chr7.2:12014524-12014543 | None:intergenic | 40.0% | |
| CATAGATGGGAGAAAATAGC+TGG | - | chr7.2:12013858-12013877 | MS.gene061903:intron | 40.0% | |
| CGTAAACACTTGTGAGACTA+TGG | - | chr7.2:12013590-12013609 | MS.gene061903:CDS | 40.0% | |
| GGTCATGAATGGTTGTATTG+TGG | - | chr7.2:12014944-12014963 | MS.gene061903:CDS | 40.0% | |
| GTCATGGCTTCAAATGTGAT+CGG | - | chr7.2:12014419-12014438 | MS.gene061903:intron | 40.0% | |
| TCGGTTTCTTTAGACAAGCA+AGG | + | chr7.2:12014450-12014469 | None:intergenic | 40.0% | |
| TCTGACTCCTATTCAGCTTA+TGG | - | chr7.2:12014986-12015005 | MS.gene061903:CDS | 40.0% | |
| TGTATCAGCCAGATCAATCA+AGG | + | chr7.2:12014184-12014203 | None:intergenic | 40.0% | |
| TTGGAGGAAATGGTCATGAA+TGG | - | chr7.2:12014933-12014952 | MS.gene061903:CDS | 40.0% | |
| ! | ACAACATGGCCTAAGGTATT+AGG | - | chr7.2:12014035-12014054 | MS.gene061903:intron | 40.0% |
| ! | CTTTCTTTTCCCCAATTTGG+CGG | + | chr7.2:12013898-12013917 | None:intergenic | 40.0% |
| ! | GAAAAGAATGGAAGCATACC+TGG | + | chr7.2:12013383-12013402 | None:intergenic | 40.0% |
| ! | GAAACCATGGATCGACATTT+TGG | - | chr7.2:12014679-12014698 | MS.gene061903:intron | 40.0% |
| ! | TTTCTTTTCCCCAATTTGGC+GGG | + | chr7.2:12013897-12013916 | None:intergenic | 40.0% |
| !! | GCATACCTGGTTTTCATTAC+GGG | + | chr7.2:12013370-12013389 | None:intergenic | 40.0% |
| !! | GTTTTGATCTAACTGACCAG+TGG | - | chr7.2:12014397-12014416 | MS.gene061903:intron | 40.0% |
| !! | TTTCTGGTTGGAGTTGATGA+CGG | - | chr7.2:12014280-12014299 | MS.gene061903:intron | 40.0% |
| !!! | GCAGAATGAGCTTGCTATTT+TGG | - | chr7.2:12014636-12014655 | MS.gene061903:intron | 40.0% |
| ACGAACGATTTCGCCTTCAA+GGG | + | chr7.2:12013337-12013356 | None:intergenic | 45.0% | |
| ATCTAACTGACCAGTGGTCA+TGG | - | chr7.2:12014403-12014422 | MS.gene061903:intron | 45.0% | |
| CCAAGCATGCCCTAAAACTA+GGG | + | chr7.2:12013483-12013502 | None:intergenic | 45.0% | |
| CCATCTCTTAGTCGAAACCA+TGG | - | chr7.2:12014666-12014685 | MS.gene061903:intron | 45.0% | |
| CCATGGTTTCGACTAAGAGA+TGG | + | chr7.2:12014669-12014688 | None:intergenic | 45.0% | |
| CTGCTGTGACTAAGAGTCTT+TGG | - | chr7.2:12014716-12014735 | MS.gene061903:intron | 45.0% | |
| CTTTCATACCCCGCCAAATT+GGG | - | chr7.2:12013885-12013904 | MS.gene061903:intron | 45.0% | |
| GCCAGATCAATCAAGGTGAA+CGG | + | chr7.2:12014177-12014196 | None:intergenic | 45.0% | |
| GTTGCCAAAATGTCGATCCA+TGG | + | chr7.2:12014686-12014705 | None:intergenic | 45.0% | |
| TACGAACGATTTCGCCTTCA+AGG | + | chr7.2:12013338-12013357 | None:intergenic | 45.0% | |
| TCCAAGCATGCCCTAAAACT+AGG | + | chr7.2:12013484-12013503 | None:intergenic | 45.0% | |
| TCCGTTCACCTTGATTGATC+TGG | - | chr7.2:12014173-12014192 | MS.gene061903:intron | 45.0% | |
| TTCGTCGTTCTTCAACAGTG+AGG | + | chr7.2:12013314-12013333 | None:intergenic | 45.0% | |
| TTTCATACCCCGCCAAATTG+GGG | - | chr7.2:12013886-12013905 | MS.gene061903:intron | 45.0% | |
| ! | ACATTTGAAGCCATGACCAC+TGG | + | chr7.2:12014416-12014435 | None:intergenic | 45.0% |
| ! | TTCTTTTCCCCAATTTGGCG+GGG | + | chr7.2:12013896-12013915 | None:intergenic | 45.0% |
| !! | TTAATAAAATAAAAAACTTA+GGG | - | chr7.2:12013979-12013998 | MS.gene061903:CDS | 5.0% |
| CCAATTTGGCGGGGTATGAA+AGG | + | chr7.2:12013887-12013906 | None:intergenic | 50.0% | |
| CCTTTCATACCCCGCCAAAT+TGG | - | chr7.2:12013884-12013903 | MS.gene061903:intron | 50.0% | |
| GAAGAACGACGAACCCTTGA+AGG | - | chr7.2:12013321-12013340 | MS.gene061903:CDS | 50.0% | |
| ! | GGGGATCCGATTCGTGTTAA+TGG | - | chr7.2:12013245-12013264 | MS.gene061903:CDS | 50.0% |
| !! | GGATCGACATTTTGGCAACG+TGG | - | chr7.2:12014687-12014706 | MS.gene061903:intron | 50.0% |
| !!! | CCCTAGTTTTAGGGCATGCT+TGG | - | chr7.2:12013480-12013499 | MS.gene061903:intron | 50.0% |
| CCTCGCCCGTAATGAAAACC+AGG | - | chr7.2:12013362-12013381 | MS.gene061903:intron | 55.0% | |
| GGGCATGCTTGGATCATCGA+AGG | - | chr7.2:12013491-12013510 | MS.gene061903:intron | 55.0% | |
| !! | CCTGGTTTTCATTACGGGCG+AGG | + | chr7.2:12013365-12013384 | None:intergenic | 55.0% |
| !! | GGTTGGAGTTGATGACGGCG+AGG | - | chr7.2:12014285-12014304 | MS.gene061903:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 12013243 | 12015022 | 12013243 | ID=MS.gene061903 |
| chr7.2 | mRNA | 12013243 | 12015022 | 12013243 | ID=MS.gene061903.t1;Parent=MS.gene061903 |
| chr7.2 | exon | 12014882 | 12015022 | 12014882 | ID=MS.gene061903.t1.exon1;Parent=MS.gene061903.t1 |
| chr7.2 | CDS | 12014882 | 12015022 | 12014882 | ID=cds.MS.gene061903.t1;Parent=MS.gene061903.t1 |
| chr7.2 | exon | 12013959 | 12014045 | 12013959 | ID=MS.gene061903.t1.exon2;Parent=MS.gene061903.t1 |
| chr7.2 | CDS | 12013959 | 12014045 | 12013959 | ID=cds.MS.gene061903.t1;Parent=MS.gene061903.t1 |
| chr7.2 | exon | 12013557 | 12013625 | 12013557 | ID=MS.gene061903.t1.exon3;Parent=MS.gene061903.t1 |
| chr7.2 | CDS | 12013557 | 12013625 | 12013557 | ID=cds.MS.gene061903.t1;Parent=MS.gene061903.t1 |
| chr7.2 | exon | 12013243 | 12013377 | 12013243 | ID=MS.gene061903.t1.exon4;Parent=MS.gene061903.t1 |
| chr7.2 | CDS | 12013243 | 12013377 | 12013243 | ID=cds.MS.gene061903.t1;Parent=MS.gene061903.t1 |
| Gene Sequence |
| Protein sequence |
