Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062604.t1 | XP_003619776.1 | 98.1 | 157 | 3 | 0 | 1 | 157 | 1 | 157 | 1.20E-76 | 295.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062604.t1 | Q6L4D2 | 45.5 | 143 | 64 | 3 | 8 | 149 | 12 | 141 | 3.2e-22 | 106.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062604.t1 | G7KPB9 | 98.1 | 157 | 3 | 0 | 1 | 157 | 1 | 157 | 8.4e-77 | 295.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050868 | MS.gene062604 | -0.802094 | 6.60E-49 | -1.69E-46 |
| MS.gene050871 | MS.gene062604 | -0.804556 | 2.04E-49 | -1.69E-46 |
| MS.gene051838 | MS.gene062604 | -0.806516 | 7.92E-50 | -1.69E-46 |
| MS.gene053203 | MS.gene062604 | -0.800527 | 1.38E-48 | -1.69E-46 |
| MS.gene056387 | MS.gene062604 | -0.800657 | 1.30E-48 | -1.69E-46 |
| MS.gene058157 | MS.gene062604 | -0.821599 | 3.71E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062604.t1 | MTR_6g068860 | 98.089 | 157 | 3 | 0 | 1 | 157 | 1 | 157 | 1.35e-110 | 311 |
| MS.gene062604.t1 | MTR_2g049340 | 75.524 | 143 | 35 | 0 | 1 | 143 | 6 | 148 | 8.39e-71 | 210 |
| MS.gene062604.t1 | MTR_4g117600 | 45.985 | 137 | 74 | 0 | 16 | 152 | 21 | 157 | 3.78e-35 | 120 |
| MS.gene062604.t1 | MTR_1g094035 | 42.857 | 154 | 73 | 5 | 2 | 154 | 6 | 145 | 3.83e-27 | 100 |
| MS.gene062604.t1 | MTR_4g117600 | 46.939 | 98 | 52 | 0 | 16 | 113 | 21 | 118 | 3.65e-23 | 88.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062604.t1 | AT5G46530 | 71.812 | 149 | 42 | 0 | 1 | 149 | 6 | 154 | 1.77e-76 | 225 |
| MS.gene062604.t1 | AT1G29520 | 78.523 | 149 | 32 | 0 | 1 | 149 | 6 | 154 | 1.64e-75 | 222 |
| MS.gene062604.t1 | AT5G18970 | 43.506 | 154 | 87 | 0 | 2 | 155 | 7 | 160 | 1.07e-37 | 127 |
| MS.gene062604.t1 | AT1G04560 | 43.590 | 156 | 74 | 4 | 2 | 156 | 7 | 149 | 8.11e-31 | 110 |
Find 29 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTCCCATTGGGAAGAAAA+TGG | 0.238328 | 6.4:-47325021 | None:intergenic |
| TGCATGTATGTCATTGTTCT+AGG | 0.248721 | 6.4:+47324747 | MS.gene062604:CDS |
| CTCTACATAGTTGCAATTCA+TGG | 0.272940 | 6.4:+47325978 | MS.gene062604:CDS |
| TTGAAGCTCAATCTCTTTCC+AGG | 0.305770 | 6.4:-47325809 | None:intergenic |
| GGTGCTGCATCGACAATTTC+AGG | 0.318250 | 6.4:+47325092 | MS.gene062604:CDS |
| TGCTGCTTCAATTGCTACCT+TGG | 0.341425 | 6.4:+47325157 | MS.gene062604:CDS |
| CCAATGGGAAATGCTTCAAC+TGG | 0.374176 | 6.4:+47325032 | MS.gene062604:CDS |
| CTTCAATTGCTACCTTGGCT+TGG | 0.378873 | 6.4:+47325162 | MS.gene062604:CDS |
| GACTCTTACACTGCTTGCCA+TGG | 0.388463 | 6.4:+47325184 | MS.gene062604:CDS |
| GTTACGATTTCTTTGTTAGC+TGG | 0.398205 | 6.4:+47325062 | MS.gene062604:CDS |
| TCTTTGTTAGCTGGAGTAGT+TGG | 0.415192 | 6.4:+47325071 | MS.gene062604:CDS |
| TTGTTCTAGGCATTGGTGCA+TGG | 0.424644 | 6.4:+47324760 | MS.gene062604:CDS |
| CAGTTGAAGCATTTCCCATT+GGG | 0.433142 | 6.4:-47325031 | None:intergenic |
| TGAGCTGGAAGATTTAATTC+CGG | 0.439380 | 6.4:-47324990 | None:intergenic |
| TGTTCTAGGCATTGGTGCAT+GGG | 0.449144 | 6.4:+47324761 | MS.gene062604:CDS |
| ACTCTTACACTGCTTGCCAT+GGG | 0.453490 | 6.4:+47325185 | MS.gene062604:CDS |
| TTTCCCATTGGGAAGAAAAT+GGG | 0.461124 | 6.4:-47325020 | None:intergenic |
| GAGTCAAACATATCTGTTCA+TGG | 0.465531 | 6.4:+47325114 | MS.gene062604:CDS |
| TCAAATTAGAAATGCACGTC+TGG | 0.465623 | 6.4:+47325828 | MS.gene062604:CDS |
| CCAGTTGAAGCATTTCCCAT+TGG | 0.492597 | 6.4:-47325032 | None:intergenic |
| GATAATTATGAAAGCCTCCA+TGG | 0.495765 | 6.4:-47325937 | None:intergenic |
| TATGTCATTGTTCTAGGCAT+TGG | 0.506725 | 6.4:+47324753 | MS.gene062604:CDS |
| GTCCATAGCAACACTTCTCT+TGG | 0.510981 | 6.4:+47324713 | MS.gene062604:CDS |
| CACCAAGAGAAGTGTTGCTA+TGG | 0.525202 | 6.4:-47324715 | None:intergenic |
| ATGAATAGAGCAATAGATCA+TGG | 0.550315 | 6.4:+47324786 | MS.gene062604:CDS |
| AGAGAAGCTGTGTAACTGAA+AGG | 0.596446 | 6.4:-47325959 | None:intergenic |
| CTCTTACACTGCTTGCCATG+GGG | 0.615745 | 6.4:+47325186 | MS.gene062604:CDS |
| GTAGCAATTGAAGCAGCAGA+TGG | 0.618253 | 6.4:-47325152 | None:intergenic |
| CAGTGTAAGAGTCCAAGCCA+AGG | 0.634224 | 6.4:-47325174 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTAAATGAGCATATTAA+AGG | + | chr6.4:47325506-47325525 | MS.gene062604:intron | 15.0% |
| !! | AATTAAATGAGCATATTAAA+GGG | + | chr6.4:47325507-47325526 | MS.gene062604:intron | 15.0% |
| !!! | GAATCATTTAATAATTTTCT+TGG | + | chr6.4:47325864-47325883 | MS.gene062604:intron | 15.0% |
| !!! | GTTTTAATTGAAAACAAATT+TGG | - | chr6.4:47325726-47325745 | None:intergenic | 15.0% |
| !! | CTCCAAATAATAGTAAAAAA+TGG | + | chr6.4:47325250-47325269 | MS.gene062604:intron | 20.0% |
| !!! | ATTTTGTTATGAAATTATGC+AGG | + | chr6.4:47324966-47324985 | MS.gene062604:intron | 20.0% |
| !!! | CATTTTTTACTATTATTTGG+AGG | - | chr6.4:47325252-47325271 | None:intergenic | 20.0% |
| !!! | TGCCATTTTTTACTATTATT+TGG | - | chr6.4:47325255-47325274 | None:intergenic | 20.0% |
| !!! | TTCTGACATTCTAAAATATT+TGG | - | chr6.4:47324854-47324873 | None:intergenic | 20.0% |
| ! | CTATCAATCATCTATAAGAA+TGG | - | chr6.4:47325437-47325456 | None:intergenic | 25.0% |
| ! | TATTACTTGTGTAGAAAACA+TGG | - | chr6.4:47325356-47325375 | None:intergenic | 25.0% |
| ! | TTAGAATGTCAGAAAAACAA+TGG | + | chr6.4:47324860-47324879 | MS.gene062604:intron | 25.0% |
| ! | TTTACTATTATTTGGAGGTA+TGG | - | chr6.4:47325247-47325266 | None:intergenic | 25.0% |
| !! | TATCTTACCTAGTTTAAGTT+TGG | + | chr6.4:47325611-47325630 | MS.gene062604:intron | 25.0% |
| !! | TGTAACTTTTGTCAAAATCT+CGG | + | chr6.4:47325692-47325711 | MS.gene062604:intron | 25.0% |
| !!! | ATAGATCATGGTTTTATCAT+AGG | + | chr6.4:47324798-47324817 | MS.gene062604:CDS | 25.0% |
| AATAGTAAAAAATGGCAACC+CGG | + | chr6.4:47325258-47325277 | MS.gene062604:intron | 30.0% | |
| ATGAATAGAGCAATAGATCA+TGG | + | chr6.4:47324786-47324805 | MS.gene062604:CDS | 30.0% | |
| ATTTGGCAAATGTGAAAGAT+AGG | - | chr6.4:47324837-47324856 | None:intergenic | 30.0% | |
| ! | TACCTAGTTTAAGTTTGGAT+TGG | + | chr6.4:47325616-47325635 | MS.gene062604:intron | 30.0% |
| !!! | GTATGCTGAAGTTTTAATTC+CGG | - | chr6.4:47324944-47324963 | None:intergenic | 30.0% |
| AAAATGGGTGAAAAATGAGC+TGG | - | chr6.4:47325008-47325027 | None:intergenic | 35.0% | |
| AAAGAGAGAAATTTACCCCA+TGG | - | chr6.4:47325204-47325223 | None:intergenic | 35.0% | |
| ATTTCCCATTGGGAAGAAAA+TGG | - | chr6.4:47325024-47325043 | None:intergenic | 35.0% | |
| ATTTGGCAAAATCATGGTAG+TGG | + | chr6.4:47325645-47325664 | MS.gene062604:intron | 35.0% | |
| CGATGAATTTGGCAAAATCA+TGG | + | chr6.4:47325639-47325658 | MS.gene062604:intron | 35.0% | |
| CTCTACATAGTTGCAATTCA+TGG | + | chr6.4:47325978-47325997 | MS.gene062604:CDS | 35.0% | |
| GAGTCAAACATATCTGTTCA+TGG | + | chr6.4:47325114-47325133 | MS.gene062604:CDS | 35.0% | |
| GATAATTATGAAAGCCTCCA+TGG | - | chr6.4:47325940-47325959 | None:intergenic | 35.0% | |
| GTTACGATTTCTTTGTTAGC+TGG | + | chr6.4:47325062-47325081 | MS.gene062604:CDS | 35.0% | |
| GTTATGAAATTATGCAGGTC+CGG | + | chr6.4:47324971-47324990 | MS.gene062604:intron | 35.0% | |
| TCAAATTAGAAATGCACGTC+TGG | + | chr6.4:47325828-47325847 | MS.gene062604:CDS | 35.0% | |
| TGAGCTGGAAGATTTAATTC+CGG | - | chr6.4:47324993-47325012 | None:intergenic | 35.0% | |
| TGCATGTATGTCATTGTTCT+AGG | + | chr6.4:47324747-47324766 | MS.gene062604:CDS | 35.0% | |
| TTTCCCATTGGGAAGAAAAT+GGG | - | chr6.4:47325023-47325042 | None:intergenic | 35.0% | |
| ! | TATGTCATTGTTCTAGGCAT+TGG | + | chr6.4:47324753-47324772 | MS.gene062604:CDS | 35.0% |
| ! | TCTCTTTTTGCAGAAAACCA+TGG | + | chr6.4:47325920-47325939 | MS.gene062604:intron | 35.0% |
| AAAATAATGAGACCCCTTCC+CGG | - | chr6.4:47325312-47325331 | None:intergenic | 40.0% | |
| AACGTGTGAGTTAGGTTTAC+CGG | + | chr6.4:47324917-47324936 | MS.gene062604:intron | 40.0% | |
| AGAGAAGCTGTGTAACTGAA+AGG | - | chr6.4:47325962-47325981 | None:intergenic | 40.0% | |
| CGCCAATCCAAACTTAAACT+AGG | - | chr6.4:47325621-47325640 | None:intergenic | 40.0% | |
| TCTTTGTTAGCTGGAGTAGT+TGG | + | chr6.4:47325071-47325090 | MS.gene062604:CDS | 40.0% | |
| TTGAAGCTCAATCTCTTTCC+AGG | - | chr6.4:47325812-47325831 | None:intergenic | 40.0% | |
| ! | CAGTTGAAGCATTTCCCATT+GGG | - | chr6.4:47325034-47325053 | None:intergenic | 40.0% |
| ! | CTTTTTGCAGAAAACCATGG+AGG | + | chr6.4:47325923-47325942 | MS.gene062604:intron | 40.0% |
| ! | TCACCCATTTTCTTCCCAAT+GGG | + | chr6.4:47325017-47325036 | MS.gene062604:CDS | 40.0% |
| ! | TTAACTTTTGCAGCTTTGCC+TGG | + | chr6.4:47325791-47325810 | MS.gene062604:intron | 40.0% |
| ! | TTCACCCATTTTCTTCCCAA+TGG | + | chr6.4:47325016-47325035 | MS.gene062604:CDS | 40.0% |
| !! | GTTTGGATTGGCGATGAATT+TGG | + | chr6.4:47325628-47325647 | MS.gene062604:intron | 40.0% |
| !!! | CTGAAGTTTTAATTCCGGTC+CGG | - | chr6.4:47324939-47324958 | None:intergenic | 40.0% |
| ACTCTTACACTGCTTGCCAT+GGG | + | chr6.4:47325185-47325204 | MS.gene062604:CDS | 45.0% | |
| CCAATGGGAAATGCTTCAAC+TGG | + | chr6.4:47325032-47325051 | MS.gene062604:CDS | 45.0% | |
| CTTCAATTGCTACCTTGGCT+TGG | + | chr6.4:47325162-47325181 | MS.gene062604:CDS | 45.0% | |
| GTAGCAATTGAAGCAGCAGA+TGG | - | chr6.4:47325155-47325174 | None:intergenic | 45.0% | |
| GTCCATAGCAACACTTCTCT+TGG | + | chr6.4:47324713-47324732 | MS.gene062604:CDS | 45.0% | |
| TGCGTAGTAACGTGTGAGTT+AGG | + | chr6.4:47324909-47324928 | MS.gene062604:intron | 45.0% | |
| ! | CCAGTTGAAGCATTTCCCAT+TGG | - | chr6.4:47325035-47325054 | None:intergenic | 45.0% |
| ! | TGCTGCTTCAATTGCTACCT+TGG | + | chr6.4:47325157-47325176 | MS.gene062604:CDS | 45.0% |
| !! | CACCAAGAGAAGTGTTGCTA+TGG | - | chr6.4:47324718-47324737 | None:intergenic | 45.0% |
| !! | TGTTCTAGGCATTGGTGCAT+GGG | + | chr6.4:47324761-47324780 | MS.gene062604:CDS | 45.0% |
| !! | TTGTTCTAGGCATTGGTGCA+TGG | + | chr6.4:47324760-47324779 | MS.gene062604:CDS | 45.0% |
| CAGTGTAAGAGTCCAAGCCA+AGG | - | chr6.4:47325177-47325196 | None:intergenic | 50.0% | |
| CTCTTACACTGCTTGCCATG+GGG | + | chr6.4:47325186-47325205 | MS.gene062604:CDS | 50.0% | |
| GACTCTTACACTGCTTGCCA+TGG | + | chr6.4:47325184-47325203 | MS.gene062604:CDS | 50.0% | |
| GTGAGTTAGGTTTACCGGAC+CGG | + | chr6.4:47324922-47324941 | MS.gene062604:intron | 50.0% | |
| ! | GGTGCTGCATCGACAATTTC+AGG | + | chr6.4:47325092-47325111 | MS.gene062604:CDS | 50.0% |
| AACCCGGTGCACTAAAGCTC+CGG | + | chr6.4:47325274-47325293 | MS.gene062604:intron | 55.0% | |
| CTAAAGCTCCGGCATACGCA+CGG | + | chr6.4:47325285-47325304 | MS.gene062604:intron | 55.0% | |
| ! | ATGCCGGAGCTTTAGTGCAC+CGG | - | chr6.4:47325280-47325299 | None:intergenic | 55.0% |
| ! | TGCCGGAGCTTTAGTGCACC+GGG | - | chr6.4:47325279-47325298 | None:intergenic | 60.0% |
| CATACGCACGGTCCGGGAAG+GGG | + | chr6.4:47325297-47325316 | MS.gene062604:intron | 65.0% | |
| GCATACGCACGGTCCGGGAA+GGG | + | chr6.4:47325296-47325315 | MS.gene062604:intron | 65.0% | |
| TTCCCGGACCGTGCGTATGC+CGG | - | chr6.4:47325296-47325315 | None:intergenic | 65.0% | |
| CTCCGGCATACGCACGGTCC+GGG | + | chr6.4:47325291-47325310 | MS.gene062604:intron | 70.0% | |
| GCTCCGGCATACGCACGGTC+CGG | + | chr6.4:47325290-47325309 | MS.gene062604:intron | 70.0% | |
| GGCATACGCACGGTCCGGGA+AGG | + | chr6.4:47325295-47325314 | MS.gene062604:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 47324708 | 47326028 | 47324708 | ID=MS.gene062604 |
| chr6.4 | mRNA | 47324708 | 47326028 | 47324708 | ID=MS.gene062604.t1;Parent=MS.gene062604 |
| chr6.4 | exon | 47324708 | 47324819 | 47324708 | ID=MS.gene062604.t1.exon1;Parent=MS.gene062604.t1 |
| chr6.4 | CDS | 47324708 | 47324819 | 47324708 | ID=cds.MS.gene062604.t1;Parent=MS.gene062604.t1 |
| chr6.4 | exon | 47324988 | 47325207 | 47324988 | ID=MS.gene062604.t1.exon2;Parent=MS.gene062604.t1 |
| chr6.4 | CDS | 47324988 | 47325207 | 47324988 | ID=cds.MS.gene062604.t1;Parent=MS.gene062604.t1 |
| chr6.4 | exon | 47325804 | 47325849 | 47325804 | ID=MS.gene062604.t1.exon3;Parent=MS.gene062604.t1 |
| chr6.4 | CDS | 47325804 | 47325849 | 47325804 | ID=cds.MS.gene062604.t1;Parent=MS.gene062604.t1 |
| chr6.4 | exon | 47325933 | 47326028 | 47325933 | ID=MS.gene062604.t1.exon4;Parent=MS.gene062604.t1 |
| chr6.4 | CDS | 47325933 | 47326028 | 47325933 | ID=cds.MS.gene062604.t1;Parent=MS.gene062604.t1 |
| Gene Sequence |
| Protein sequence |
