Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063045.t1 | XP_003615024.1 | 99.5 | 386 | 2 | 0 | 1 | 386 | 1 | 386 | 9.20E-224 | 785.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063045.t1 | Q803I2 | 52.7 | 393 | 162 | 4 | 5 | 386 | 4 | 383 | 1.8e-119 | 430.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063045.t1 | G7KA80 | 99.5 | 386 | 2 | 0 | 1 | 386 | 1 | 386 | 6.6e-224 | 785.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060595 | MS.gene063045 | 0.804365 | 2.24E-49 | -1.69E-46 |
| MS.gene060929 | MS.gene063045 | 0.829658 | 4.55E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene25743 | MS.gene063045 | PPI |
| MS.gene69767 | MS.gene063045 | PPI |
| MS.gene70505 | MS.gene063045 | PPI |
| MS.gene044295 | MS.gene063045 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063045.t1 | MTR_5g062720 | 99.482 | 386 | 2 | 0 | 1 | 386 | 1 | 386 | 0.0 | 802 |
| MS.gene063045.t1 | MTR_5g062720 | 99.340 | 303 | 2 | 0 | 1 | 303 | 1 | 303 | 0.0 | 634 |
| MS.gene063045.t1 | MTR_4g094255 | 68.312 | 385 | 120 | 1 | 1 | 385 | 1 | 383 | 0.0 | 592 |
| MS.gene063045.t1 | MTR_4g094255 | 66.554 | 296 | 97 | 1 | 90 | 385 | 1 | 294 | 7.10e-159 | 448 |
| MS.gene063045.t1 | MTR_3g081480 | 36.148 | 379 | 199 | 5 | 8 | 378 | 7 | 350 | 2.33e-76 | 240 |
| MS.gene063045.t1 | MTR_4g080800 | 32.683 | 205 | 97 | 9 | 202 | 384 | 290 | 475 | 1.74e-19 | 90.1 |
| MS.gene063045.t1 | MTR_4g080800 | 38.318 | 107 | 65 | 1 | 6 | 111 | 5 | 111 | 9.51e-16 | 79.0 |
| MS.gene063045.t1 | MTR_2g083260 | 28.470 | 281 | 159 | 10 | 124 | 384 | 220 | 478 | 1.65e-18 | 87.4 |
| MS.gene063045.t1 | MTR_2g083260 | 37.273 | 110 | 68 | 1 | 3 | 111 | 2 | 111 | 3.36e-15 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063045.t1 | AT1G36050 | 81.865 | 386 | 70 | 0 | 1 | 386 | 1 | 386 | 0.0 | 697 |
| MS.gene063045.t1 | AT1G36050 | 81.675 | 382 | 70 | 0 | 1 | 382 | 1 | 382 | 0.0 | 689 |
| MS.gene063045.t1 | AT1G22200 | 77.979 | 386 | 85 | 0 | 1 | 386 | 1 | 386 | 0.0 | 656 |
| MS.gene063045.t1 | AT1G22200 | 77.015 | 335 | 77 | 0 | 1 | 335 | 1 | 335 | 0.0 | 565 |
| MS.gene063045.t1 | AT3G22290 | 36.031 | 383 | 202 | 10 | 4 | 379 | 3 | 349 | 4.72e-75 | 237 |
| MS.gene063045.t1 | AT3G22290 | 36.031 | 383 | 202 | 10 | 4 | 379 | 3 | 349 | 4.72e-75 | 237 |
| MS.gene063045.t1 | AT4G27080 | 31.841 | 201 | 104 | 7 | 202 | 384 | 293 | 478 | 1.97e-21 | 96.3 |
| MS.gene063045.t1 | AT4G27080 | 37.963 | 108 | 66 | 1 | 6 | 112 | 5 | 112 | 5.87e-16 | 79.7 |
| MS.gene063045.t1 | AT4G27080 | 31.841 | 201 | 104 | 7 | 202 | 384 | 345 | 530 | 3.38e-21 | 95.5 |
| MS.gene063045.t1 | AT4G27080 | 38.614 | 101 | 61 | 1 | 13 | 112 | 64 | 164 | 1.04e-13 | 72.8 |
| MS.gene063045.t1 | AT3G20560 | 30.693 | 202 | 108 | 7 | 202 | 384 | 293 | 481 | 5.13e-18 | 85.9 |
| MS.gene063045.t1 | AT3G20560 | 40.187 | 107 | 63 | 1 | 7 | 112 | 6 | 112 | 1.82e-17 | 84.3 |
| MS.gene063045.t1 | AT1G50950 | 26.690 | 281 | 167 | 9 | 124 | 384 | 221 | 482 | 6.70e-17 | 82.4 |
| MS.gene063045.t1 | AT1G50950 | 39.815 | 108 | 64 | 1 | 6 | 112 | 5 | 112 | 1.40e-16 | 81.6 |
| MS.gene063045.t1 | AT1G50950 | 26.690 | 281 | 167 | 9 | 124 | 384 | 226 | 487 | 6.74e-17 | 82.4 |
| MS.gene063045.t1 | AT1G50950 | 39.815 | 108 | 64 | 1 | 6 | 112 | 5 | 112 | 1.49e-16 | 81.6 |
Find 81 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTATAGTCGCACTCTTTC+TGG | 0.133582 | 5.3:-49420160 | MS.gene063045:CDS |
| CTACGACCTTTCTCCAATTA+AGG | 0.195998 | 5.3:-49410451 | MS.gene063045:intron |
| ATTACGAAGCTTGTTCATTA+TGG | 0.253518 | 5.3:+49420213 | None:intergenic |
| TCTTCTTCTTGATAGCCTTT+TGG | 0.307819 | 5.3:+49409424 | None:intergenic |
| GGCCGATTGCAGTCCCTCCC+TGG | 0.324323 | 5.3:-49410486 | MS.gene063045:CDS |
| CATTTGTATACCTCCAACTA+TGG | 0.339863 | 5.3:+49409610 | None:intergenic |
| TCTCTGTGACAGAACACTTT+AGG | 0.345410 | 5.3:-49410521 | MS.gene063045:CDS |
| TACCGCCTCAGCACCATAAC+AGG | 0.379587 | 5.3:+49418098 | None:intergenic |
| CTAGATTCATTTATCTATCA+TGG | 0.385708 | 5.3:-49409447 | MS.gene063045:CDS |
| GCCGATTGCAGTCCCTCCCT+GGG | 0.388413 | 5.3:-49410485 | MS.gene063045:CDS |
| GGGAAAAGCTTTCAGCAATC+TGG | 0.414672 | 5.3:-49415847 | MS.gene063045:CDS |
| CCCCAGGGAGGGACTGCAAT+CGG | 0.422047 | 5.3:+49410484 | None:intergenic |
| CAAACCTGGTCAATTGAATC+CGG | 0.433588 | 5.3:+49416821 | None:intergenic |
| GATCAATAACCTGGTTTGAC+TGG | 0.437886 | 5.3:+49410619 | None:intergenic |
| ACTTATTGCGGATCCTGTTA+TGG | 0.450203 | 5.3:-49418111 | MS.gene063045:CDS |
| TTGGAAGTAAACAAGGTGGC+AGG | 0.459413 | 5.3:-49415889 | MS.gene063045:CDS |
| CGTACATGTGCATGACCTGC+TGG | 0.468545 | 5.3:-49415824 | MS.gene063045:CDS |
| CTGCCTCCATGTCTTTGCAA+GGG | 0.472630 | 5.3:+49418150 | None:intergenic |
| GAAAAGCAAGAGCATGAGAA+TGG | 0.476070 | 5.3:+49420114 | None:intergenic |
| CACATCAACAGAATAGCCTT+TGG | 0.482453 | 5.3:-49413424 | MS.gene063045:CDS |
| CCTGCCTCCATGTCTTTGCA+AGG | 0.483999 | 5.3:+49418149 | None:intergenic |
| CATTAGCGGAGAACAACATC+TGG | 0.491394 | 5.3:-49418764 | MS.gene063045:CDS |
| GAAAGAGAAATTACGAAGCT+CGG | 0.495749 | 5.3:+49420087 | None:intergenic |
| GAAAGAATCAAGGAAGAAGA+AGG | 0.501222 | 5.3:-49415937 | MS.gene063045:CDS |
| AAGCATATCGAAAGAAAGGT+TGG | 0.504184 | 5.3:-49416856 | MS.gene063045:CDS |
| CGTGAAGCATATCGAAAGAA+AGG | 0.504663 | 5.3:-49416860 | MS.gene063045:CDS |
| GAAACAAGGCAAGATGGAAT+CGG | 0.513498 | 5.3:-49418574 | MS.gene063045:CDS |
| ATTTATCTATCATGGCCAAA+AGG | 0.514131 | 5.3:-49409439 | MS.gene063045:CDS |
| GTGATAGAAACAAGGCAAGA+TGG | 0.530527 | 5.3:-49418580 | MS.gene063045:CDS |
| ACCAATGTGTGTGCCATAGT+TGG | 0.531594 | 5.3:-49409623 | MS.gene063045:CDS |
| TCTTGATTATCAGTGTGCAT+TGG | 0.532026 | 5.3:-49413280 | MS.gene063045:intron |
| AAAACACCTTAATTGGAGAA+AGG | 0.535317 | 5.3:+49410445 | None:intergenic |
| AAAGGCTATCAAGAAGAAGA+TGG | 0.538983 | 5.3:-49409421 | MS.gene063045:CDS |
| TTGTTCTCCGCTAATGTCCA+TGG | 0.540332 | 5.3:+49418771 | None:intergenic |
| CTCGATGCCATGGACATTAG+CGG | 0.540952 | 5.3:-49418778 | MS.gene063045:CDS |
| GTCAAGAGGGTTTACAACAC+CGG | 0.543744 | 5.3:+49413390 | None:intergenic |
| CTCACGTCAGTGTACATAGT+TGG | 0.544532 | 5.3:+49410654 | None:intergenic |
| ATCACTTAGTGCAAAAGAGA+AGG | 0.545278 | 5.3:-49415967 | MS.gene063045:intron |
| TCCAACTATGGCACACACAT+TGG | 0.549757 | 5.3:+49409622 | None:intergenic |
| AGGGTTCTTAGAAAGAATCA+AGG | 0.554292 | 5.3:-49415947 | MS.gene063045:CDS |
| AAAATACTGATACATCCCAC+TGG | 0.555074 | 5.3:+49413244 | None:intergenic |
| AGCATATCGAAAGAAAGGTT+GGG | 0.557532 | 5.3:-49416855 | MS.gene063045:CDS |
| CCTTGCAAAGACATGGAGGC+AGG | 0.557759 | 5.3:-49418149 | MS.gene063045:CDS |
| AGGCTATTCTGTTGATGTGG+TGG | 0.558845 | 5.3:+49413428 | None:intergenic |
| AGTATAGAACACGCTAGTGC+AGG | 0.559960 | 5.3:+49418805 | None:intergenic |
| TATACTTAGTCTCGATGCCA+TGG | 0.561660 | 5.3:-49418788 | MS.gene063045:CDS |
| GGTGAAGGATGCAATGTGTA+TGG | 0.569301 | 5.3:-49415916 | MS.gene063045:CDS |
| AAGAAAAGAATAGACTCTCA+TGG | 0.572185 | 5.3:-49418607 | MS.gene063045:CDS |
| GTGTTGTAAACCCTCTTGAC+CGG | 0.574958 | 5.3:-49413387 | MS.gene063045:intron |
| AGGTTGGGCACTCTCAAGCC+CGG | 0.575134 | 5.3:-49416840 | MS.gene063045:CDS |
| AAACCTGGTCAATTGAATCC+GGG | 0.575289 | 5.3:+49416822 | None:intergenic |
| AAGCCCGGATTCAATTGACC+AGG | 0.575812 | 5.3:-49416825 | MS.gene063045:intron |
| ATCAAGGAAGAAGAAGGTGA+AGG | 0.577027 | 5.3:-49415931 | MS.gene063045:CDS |
| CAAAGGCTATTCTGTTGATG+TGG | 0.582521 | 5.3:+49413425 | None:intergenic |
| TAAAGAGCTCACCGGTCAAG+AGG | 0.585229 | 5.3:+49413376 | None:intergenic |
| TCAAGAGGGTTTACAACACC+GGG | 0.589163 | 5.3:+49413391 | None:intergenic |
| TGCTTCACGAACTTCCTCAC+AGG | 0.590612 | 5.3:+49416874 | None:intergenic |
| TCACTTAGTGCAAAAGAGAA+GGG | 0.593153 | 5.3:-49415966 | MS.gene063045:intron |
| TGGAAGTAAACAAGGTGGCA+GGG | 0.593479 | 5.3:-49415888 | MS.gene063045:CDS |
| ATCAAGAAGAAGATGGAACT+CGG | 0.594892 | 5.3:-49409414 | MS.gene063045:CDS |
| TTTGCGCTTGCAGTCAGATG+AGG | 0.596325 | 5.3:-49416909 | MS.gene063045:intron |
| CACTTTAGGACGGCAGATGT+AGG | 0.596476 | 5.3:-49410507 | MS.gene063045:CDS |
| CTTGTTGTAGATACTTCTAG+AGG | 0.598128 | 5.3:-49419950 | MS.gene063045:CDS |
| GAGCACAATGAGACTTATTG+CGG | 0.606344 | 5.3:-49418123 | MS.gene063045:CDS |
| CTATGTACACTGACGTGAGT+GGG | 0.606656 | 5.3:-49410650 | MS.gene063045:CDS |
| AAAGAGCTCACCGGTCAAGA+GGG | 0.621153 | 5.3:+49413377 | None:intergenic |
| ATCCTGTTATGGTGCTGAGG+CGG | 0.626803 | 5.3:-49418100 | MS.gene063045:intron |
| TATAGTCGCACTCTTTCTGG+CGG | 0.635746 | 5.3:-49420157 | MS.gene063045:CDS |
| GAACACTATCCAGTCAAACC+AGG | 0.636074 | 5.3:-49410628 | MS.gene063045:intron |
| ACCGGGAAAATAGTCTCCAA+AGG | 0.641478 | 5.3:+49413408 | None:intergenic |
| CGGATCCTGTTATGGTGCTG+AGG | 0.643851 | 5.3:-49418103 | MS.gene063045:CDS |
| AAATACTGATACATCCCACT+GGG | 0.661329 | 5.3:+49413245 | None:intergenic |
| TGTGACAGAACACTTTAGGA+CGG | 0.663702 | 5.3:-49410517 | MS.gene063045:CDS |
| ACTATGTACACTGACGTGAG+TGG | 0.665706 | 5.3:-49410651 | MS.gene063045:CDS |
| CCGATTGCAGTCCCTCCCTG+GGG | 0.667693 | 5.3:-49410484 | MS.gene063045:CDS |
| AATGTGTGTGCCATAGTTGG+AGG | 0.674458 | 5.3:-49409620 | MS.gene063045:intron |
| ATGGCAATGTGATAGAAACA+AGG | 0.687509 | 5.3:-49418588 | MS.gene063045:CDS |
| AAACCCTTGCAAAGACATGG+AGG | 0.692409 | 5.3:-49418153 | MS.gene063045:CDS |
| GGACACAAGAAACACCCAGT+GGG | 0.693082 | 5.3:-49413259 | MS.gene063045:CDS |
| TGGACACAAGAAACACCCAG+TGG | 0.786125 | 5.3:-49413260 | MS.gene063045:CDS |
| GGAATGTTGTAATTCCTGTG+AGG | 0.801380 | 5.3:-49416888 | MS.gene063045:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 49409401 | 49420242 | 49409401 | ID=MS.gene063045 |
| chr5.3 | mRNA | 49409401 | 49420242 | 49409401 | ID=MS.gene063045.t1;Parent=MS.gene063045 |
| chr5.3 | exon | 49420101 | 49420242 | 49420101 | ID=MS.gene063045.t1.exon1;Parent=MS.gene063045.t1 |
| chr5.3 | CDS | 49420101 | 49420242 | 49420101 | ID=cds.MS.gene063045.t1;Parent=MS.gene063045.t1 |
| chr5.3 | exon | 49419931 | 49420004 | 49419931 | ID=MS.gene063045.t1.exon2;Parent=MS.gene063045.t1 |
| chr5.3 | CDS | 49419931 | 49420004 | 49419931 | ID=cds.MS.gene063045.t1;Parent=MS.gene063045.t1 |
| chr5.3 | exon | 49418759 | 49418842 | 49418759 | ID=MS.gene063045.t1.exon3;Parent=MS.gene063045.t1 |
| chr5.3 | CDS | 49418759 | 49418842 | 49418759 | ID=cds.MS.gene063045.t1;Parent=MS.gene063045.t1 |
| chr5.3 | exon | 49418564 | 49418644 | 49418564 | ID=MS.gene063045.t1.exon4;Parent=MS.gene063045.t1 |
| chr5.3 | CDS | 49418564 | 49418644 | 49418564 | ID=cds.MS.gene063045.t1;Parent=MS.gene063045.t1 |
| chr5.3 | exon | 49418101 | 49418181 | 49418101 | ID=MS.gene063045.t1.exon5;Parent=MS.gene063045.t1 |
| chr5.3 | CDS | 49418101 | 49418181 | 49418101 | ID=cds.MS.gene063045.t1;Parent=MS.gene063045.t1 |
| chr5.3 | exon | 49416826 | 49416918 | 49416826 | ID=MS.gene063045.t1.exon6;Parent=MS.gene063045.t1 |
| chr5.3 | CDS | 49416826 | 49416918 | 49416826 | ID=cds.MS.gene063045.t1;Parent=MS.gene063045.t1 |
| chr5.3 | exon | 49415801 | 49415980 | 49415801 | ID=MS.gene063045.t1.exon7;Parent=MS.gene063045.t1 |
| chr5.3 | CDS | 49415801 | 49415980 | 49415801 | ID=cds.MS.gene063045.t1;Parent=MS.gene063045.t1 |
| chr5.3 | exon | 49413388 | 49413455 | 49413388 | ID=MS.gene063045.t1.exon8;Parent=MS.gene063045.t1 |
| chr5.3 | CDS | 49413388 | 49413455 | 49413388 | ID=cds.MS.gene063045.t1;Parent=MS.gene063045.t1 |
| chr5.3 | exon | 49413238 | 49413289 | 49413238 | ID=MS.gene063045.t1.exon9;Parent=MS.gene063045.t1 |
| chr5.3 | CDS | 49413238 | 49413289 | 49413238 | ID=cds.MS.gene063045.t1;Parent=MS.gene063045.t1 |
| chr5.3 | exon | 49410629 | 49410682 | 49410629 | ID=MS.gene063045.t1.exon10;Parent=MS.gene063045.t1 |
| chr5.3 | CDS | 49410629 | 49410682 | 49410629 | ID=cds.MS.gene063045.t1;Parent=MS.gene063045.t1 |
| chr5.3 | exon | 49410452 | 49410544 | 49410452 | ID=MS.gene063045.t1.exon11;Parent=MS.gene063045.t1 |
| chr5.3 | CDS | 49410452 | 49410544 | 49410452 | ID=cds.MS.gene063045.t1;Parent=MS.gene063045.t1 |
| chr5.3 | exon | 49409621 | 49409687 | 49409621 | ID=MS.gene063045.t1.exon12;Parent=MS.gene063045.t1 |
| chr5.3 | CDS | 49409621 | 49409687 | 49409621 | ID=cds.MS.gene063045.t1;Parent=MS.gene063045.t1 |
| chr5.3 | exon | 49409401 | 49409492 | 49409401 | ID=MS.gene063045.t1.exon13;Parent=MS.gene063045.t1 |
| chr5.3 | CDS | 49409401 | 49409492 | 49409401 | ID=cds.MS.gene063045.t1;Parent=MS.gene063045.t1 |
| Gene Sequence |
| Protein sequence |
