AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063298

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063298.t1 MTR_1g021380 87.342 316 29 1 1 316 36 340 0.0 572
MS.gene063298.t1 MTR_1g021400 68.923 325 83 6 1 316 45 360 8.81e-154 437
MS.gene063298.t1 MTR_1g021400 91.111 45 4 0 315 359 227 271 5.06e-18 84.7
MS.gene063298.t1 MTR_1g021435 65.579 337 86 6 1 316 37 364 2.59e-150 428
MS.gene063298.t1 MTR_1g021410 66.667 327 88 7 1 316 37 353 1.59e-144 413
MS.gene063298.t1 MTR_1g021440 53.678 367 120 7 1 321 37 399 8.85e-126 367
MS.gene063298.t1 MTR_1g021330 56.083 337 116 7 1 315 41 367 1.02e-120 353
MS.gene063298.t1 MTR_1g021440 51.099 364 116 5 1 321 37 381 1.54e-119 350
MS.gene063298.t1 MTR_1g021360 55.312 320 101 6 1 316 197 478 1.04e-110 332
MS.gene063298.t1 MTR_1g021360 70.543 129 38 0 1 129 40 168 2.99e-55 188
MS.gene063298.t1 MTR_1g021320 46.763 417 116 7 1 315 197 609 1.79e-107 333
MS.gene063298.t1 MTR_1g021320 72.093 129 36 0 1 129 40 168 1.17e-55 195
MS.gene063298.t1 MTR_1g021320 35.952 331 178 10 2 316 523 835 1.01e-47 172
MS.gene063298.t1 MTR_1g021440 58.712 264 71 5 1 230 37 296 8.57e-96 287
MS.gene063298.t1 MTR_1g021350 47.633 338 111 8 1 315 36 330 6.99e-91 276
MS.gene063298.t1 MTR_1g021440 55.172 261 67 3 1 230 37 278 1.64e-89 270
MS.gene063298.t1 MTR_1g021270 37.389 337 179 9 2 315 31 358 1.51e-57 197
MS.gene063298.t1 MTR_1g021270 35.030 334 179 9 2 315 272 587 6.96e-48 171
MS.gene063298.t1 MTR_1g021290 56.316 190 69 3 1 190 170 345 3.74e-57 189
MS.gene063298.t1 MTR_1g021290 76.289 97 23 0 1 97 42 138 1.24e-45 159
MS.gene063298.t1 MTR_1g021500 33.889 360 146 13 1 293 41 375 9.87e-44 157
MS.gene063298.t1 MTR_1g021500 34.167 360 145 13 1 293 41 375 1.66e-43 157
MS.gene063298.t1 MTR_1g021295 51.724 116 52 2 201 312 102 217 3.00e-29 112
MS.gene063298.t1 MTR_7g050710 27.760 317 184 10 2 310 29 308 3.85e-23 98.6
MS.gene063298.t1 MTR_3g098790 24.843 318 175 10 2 304 29 297 4.57e-18 84.3
MS.gene063298.t1 MTR_4g068320 21.038 366 207 6 2 296 22 376 2.55e-15 77.0
MS.gene063298.t1 MTR_8g103940 23.810 315 200 11 2 310 55 335 4.59e-15 76.6
MS.gene063298.t1 MTR_7g050640 37.333 75 47 0 2 76 29 103 1.96e-12 63.9
MS.gene063298.t1 MTR_6g043890 25.260 289 160 10 18 294 47 291 2.51e-12 67.8
MS.gene063298.t1 MTR_1g035460 24.183 306 207 9 2 304 2 285 2.74e-12 67.8
MS.gene063298.t1 MTR_4g119990 25.260 289 166 12 18 294 47 297 1.29e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063298.t1 AT4G33280 31.879 298 165 9 1 293 38 302 5.93e-34 128
MS.gene063298.t1 AT4G33280 29.909 331 189 10 1 293 38 363 2.64e-33 128
MS.gene063298.t1 AT4G33280 50.459 109 53 1 1 108 38 146 1.88e-28 112
MS.gene063298.t1 AT3G18990 26.480 321 206 13 2 302 22 332 1.15e-22 97.8
MS.gene063298.t1 AT3G18990 26.480 321 206 13 2 302 22 332 1.40e-22 97.8
MS.gene063298.t1 AT4G34400 35.294 85 55 0 1 85 30 114 2.83e-15 77.0
MS.gene063298.t1 AT4G34400 35.294 85 55 0 1 85 30 114 2.83e-15 77.0
MS.gene063298.t1 AT5G18000 24.483 290 179 6 18 305 19 270 7.32e-13 68.6
MS.gene063298.t1 AT5G18000 24.315 292 177 7 18 305 54 305 1.54e-12 68.2
MS.gene063298.t1 AT5G42700 33.028 109 71 1 203 311 73 179 1.51e-11 63.2
MS.gene063298.t1 AT5G60130 37.500 88 50 2 1 83 28 115 4.12e-11 62.8
MS.gene063298.t1 AT5G60130 37.500 88 50 2 1 83 2 89 5.73e-11 63.2
MS.gene063298.t1 AT5G60130 37.500 88 50 2 1 83 28 115 7.22e-11 62.8
MS.gene063298.t1 AT5G60130 37.500 88 50 2 1 83 28 115 7.44e-11 63.2
MS.gene063298.t1 AT5G60130 37.500 88 50 2 1 83 25 112 7.87e-11 63.2

Find 0 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
8663 gene 5632 13366 5632 ID=MS.gene063298
8663 mRNA 5632 13366 5632 ID=MS.gene063298.t1;Parent=MS.gene063298
8663 exon 5632 6205 5632 ID=MS.gene063298.t1.exon1;Parent=MS.gene063298.t1
8663 CDS 5632 6205 5632 ID=cds.MS.gene063298.t1;Parent=MS.gene063298.t1
8663 exon 6573 6688 6573 ID=MS.gene063298.t1.exon2;Parent=MS.gene063298.t1
8663 CDS 6573 6688 6573 ID=cds.MS.gene063298.t1;Parent=MS.gene063298.t1
8663 exon 6841 7104 6841 ID=MS.gene063298.t1.exon3;Parent=MS.gene063298.t1
8663 CDS 6841 7104 6841 ID=cds.MS.gene063298.t1;Parent=MS.gene063298.t1
8663 exon 13244 13366 13244 ID=MS.gene063298.t1.exon4;Parent=MS.gene063298.t1
8663 CDS 13244 13366 13244 ID=cds.MS.gene063298.t1;Parent=MS.gene063298.t1
Gene Sequence

>MS.gene063298

GCACTTCCTAAAACATTCTCAGACAATTTAAAGAAGAAGTTGCCAGAAAATGTGACCCTTAAAGGTCCTAGTGGAGTTGTGTGGAATATAGGGCTGAAAACTAGAGATAATACTGTTTACTTCGTGGACGGTTGGCGTCAGTTTGCAAGAGATCATTTTCTGAAAGAGAATGATTTCCTGGTCTTTAAGTACAATGGTGAATCACTATTCAACGTTTTGACCTTTCAAGGAGAAAGCTTTTGTGAGAAAGCGGCTTCCTATTTTGTTGGAAAATGTGGGAATGCTGAAACTGAACAAAAGGACATCAGGGAGAACGAAACAAACAAGTCTGTTGAAGAAGTCAATACTGTTCTTAATGCTGGTGTTGAGTCTGCTTCCCCTGAGAAGTTCACGGCTAATGCAGTCAGTATAGTGCTTCCCTCCCAATCAACCGGAAAAAGAACCAAGCAGCCTGTTAATGAGGTTACACCTGTCCAGACTAAGAAGCGAGGAAGACCACCGAAAAAAGCTGATTATTCTGTTGAGAGAGAAGCGCATGATTATGATTTGGTGGCTTGCAACAAGGAACACTCAGGTTAAAATATTTATTTATTTTTCATTAACTTTTACTATCTTGATTTTGGCTTTTCCCGTAATGGGTCCTTTTATACTCTTTCTCAGTCATGTAATCTCATGTAACCATAATATAATCAACATAAACAAAAAATACAATTAGTTTTCCTCCTTGTGAATATTTGAAGAAAAGATTGTGATTTTCTTGACTTTGCTCATAGCCCTTTGATTTCATTCTAGGTCTCAAAACTATGGATTAAAACTCATCTGATCTTCCCTAGCAAACTGATACTTGTCTAGGAAAGAGTATTTTGCATTCAAATCCTTATATTTGTGTCAGAGCTTATTATTTTGTTTAAAATTCATGATTGAACTGTTCAATTCCACAGAGGCATCCATCAGTGGGAAGGAAGATGAGAAAAAGATAGCCCACTCATTTACCTCCTCTTTTCCATATTTTGTTATGATAATGAAATCCTGCAATATCAGTGGTTCACGTACTCTGGTGAGTTCTTCCCCTTTGTGATGCTTTGTTTTACTCCCAAATAATGTTGTTTTATCTCTCCACCCTTATCTTTATGTAACTTATGTAGTGTTTCATCTGCCTGAGAAACTAATCCAGTTGGAGTATAATATTGAATACTTGTTATATTGTAGAGAATCCCATGTCATTTCTCTGCTACACATCTCCCTGCCTGCAGAACAGAAGTTACGCTTCGCAATGCAAGAGGCGAATGTTGGACTGTGACCTCCATCCCAGTGGGAGCAATACGCGCCTTTTGTGGGGGATGGATGGCCTTTGTTTGTGATAATGGCATCAATGTTGGCGACACATGCACTTTTGAGCTCATTTCTAATTTTGTGATGAAGGTTTATATATCTGGGGTTGGAAAAGAAGGTCTTGACTATCAAAATCGGGATGTGAAACTTAATAGCTAGTTACATCTACCTGCCAATGTGCTTGTTTGTGAGACAGATAAATCAATCTTAGATGAAGATTTTTTGTGGCATGAGTTGCCAAGATTTGATACATTTTATAGATGATGATTTTTTGTGGCATGAGTTGCCGAGATACATTTTATAGATGATGATTTTTTGTTACGCTGCTGCATTTTAGTTCATTTCATTGCGAAATGTTGGTGATACGACTCAATTCGAGTAGGTACACTTCCGAATTTAGGGAGAGAAGTTAGAACAAAAAAGAGGAATTTTATTATATTTTTCATTGATTTTAATCATGAGACAATGCAATGGTTATATAAGTTAGTGCATGCTCGTAGTAAGATAATTACATCAATCGCACAATATCACTATTACCAAACAAACCAATAAAGTATGCATAAAAGATCCCTTCTAGAATAAATAATTATATGAGTTATCATTCTCTTATTTTAAGGGTTGTAAAAGAATGGTCTCAACACATGATGACCTCAAAAGCTACGTGCACATATTCTTAGATTTATACAAATCATTGCATCATTGTTTGCAAGGCAATTTACTACATCAATGTATGCAAGGGAAGCTCTATCTGGGAGCATTTTTTTTCAGCCAATGCCTTTCCAGGTGTTGCAGCATACGGCGGAACTTTGTCAGACTCACCACCCCACAATTGCCAAACAATAAAAATATCTTAAACCATATTCATTTGCAACCCAATCAGTATGAACTATCTGCCTTAAATCCTTGAAAAACTATTTGTTTTGCATAGTTTTGACTCACAGTTTCTCAAGCAAAGCAACAGTCTTAGGATCAACAAGATGAAAGGAAGTTGAGCTCCTTCGCCTTTCACGGTAGTCACTGCCCATGCAGCTAAAAAAAATTCTTCCTGACTGTAGCCATGGATAAAGAAATGGACATATAATAATGAGGTTCCAATATTGAAGGTGTATATGGCTGGTTCTTGGAAATATTTACTTTTACAGTGCCATGCTTGAACACATATTTCACACTTTTCATCACATTAAAAACAAGATGCTCCATGTAGCCAAATACATGAAAATCTTTACTTATAAATTCAATCGAACTCGACAAATTAGCAATGCTTGAGAAACCAAGGGCAGTACTCACAATAGCTCTTGCCCTCATTCACTTGCCTTCCTTTGTTGTGATCCAAAAGAACTTGTCTGCAACCTCATCCACACAATTACCAAGAGTAGCACATTGGTCTCCCTCAAGTGATAGTTGAACTTATCTGCAAGGTTCGGATTATCAAGTCTAACTTCATTAATAACACAAAAACTTGGCTAGTGTACCAAAACTCAATTGTTTCTACAACATCAACAATTGATTGAACCTCCGTAGTAGTTGAAGTTGTACCCTCTCTGAACTGTGATTGTACACGTTGATCATTTTCCGTTGCCTCCATCGTTGGAACTTTTCGCATCATAAAGCAATAATATTGGTGAGATGAATTGTCCTCAAGTATAGTATACTATCAATAGACTATAGAGGAAGAGAAGCGCTAAAATTGAAAGAGAATTTGAAAATGAGCAAAAGGAATTAGTTTTCTTTATTTTGTTCTAACTATTAGTAGGACAACATTTTGACTGAATAACCTATTTCGTCCTCAAATTTGTACGTATGGTCGTTTTTATCATCTAATTTTTCGAAATAGTCGATATGTCCATGAATTTTATAAATGTCAATCAATTTAGTCCCTTTTACAAGTCTTGGGTTAACAGTGTTGACGTGTCCCGCTAAACGCTAACGTGGAGTTCCAAGTCGGCTTCCACGGGTACTGGGACGAATTTGATCGGAAAAAAATTTAAAATTATTTTTTGGACTTTTTTTTAAAATTATTCATTAAAAATCAAGTTGGTCCCTAAATTATGTTACTTGCTATCAAATCGATCCCTGAATTATTTATCTTATCTATTAATATAATCCCTGGTACTGTCAAATAAATGTTAAAAACTAAAATAATGTAAAGAATGTGTTTAACCGTTGTTTATTACCTTTTTTCATGTTGAAATATCCATCTTATTTAAACTCGACCTTTAGCAAATTAACACCTACAAAATTTAAAAATCTTGGCATACAGTTTAGCAGCAGAACCAACTATTCAGGCATAGATGCAAAGTGTAACACAGAGAATTGATCCTAGAGCTTTATGAGTATTGGATAAGTCGTTGACAAATATATAAACTCCTAAAAAATGATACTCTAGCAAGCTCCAAACAGTTAAAAAGTCTCTGTGATATTTAAATGATATTTTCAAGAAGATGGATGTAAATGTTCTCTCTCCTGATCGATCAGGCCTTTCAGCTTTAACAACTTGTTACAAGAAATGCTTTGGCAGCGGAAAAACTTGTATTTGAAGAACTGTTGTGAAGAAATTCATGACATGGAGATCATTAATTTTCTAATATGAAAACTCAAACCACTTGTTAATTTCAAACACTAGAGGATAAATTATATCATCATAAATATAAAAGTCAAATAATTCTAAAACCCTACAAACTATTAACCACCATGGAAAGCTTTAATTTAAGATCGAATACTACACTAGGTATTTGTTTAGCACAAACCAAACTTATGCAACAAGATAATAAGCTCAAATCAGTATGATAGTTCATAAAAGTTCAAATCTTTCTGGAGAGCAGATAATTTTCAAGAGTACATAAGAAGAAACTGGTGTGATAAAAATAGCATGCAAATGAAATTATTGCAAAGAGAAATAAGTAGAGTGAAAAGAAGGTTATAACTATCTAATAATAATACATAGAAATTTGAGGATGAAATAGTTTATTCACTCATAATTTTTTTGTGCAACATGTCAAAAAGTTATAGGTGGATAACTTATTACTAAATTTAACATATATAAGATTATTTCTATAAAATAATAATGTGTTCAAATTAAATAATATCTTTCATTTAAAATATTATTTTTGAATAAAATATAAACAAAAATTATACATCTTCATATGACTATTACAAGTAAAAATAAACATTTTACATTCATTCATTAAATAATATATGTGACTTATAATAAAGATTATATACGTCGTTAATTGATTGAATTTAAGATGTAATTTTTGTTTATAATAGTGACGGGAAGAGGTAGTTAAAAAGATAAAGTTGAACTAGCCTTTCACTTCCTCGTGTACTGTTCAAGGATTAAAGCAGCCTGGAACTTTCTTTCTTGCTCGTACCCTTTCTTTCCTGCTACAACAAACTGACTGTTTCAAATGGGAGGAAGAGAAGGAGGACAAAACTGGGATGGAAGCAGATCTTGGGAGGAAGATATTTACTGGAACCATTTTCAATTCATCCATTTCACCCTATTTCTTAAAACTACCACCAATTTTCAACAACAACTTGTAAGTACTTCTTCTTTTCCTTCCATCTTCTAAAGTATTCATATTTGGGTGTTCACTGTGGAGGCAGTACATACATGCACTTCTTCCATGTACTATTTTTCATTGTTTTTACTTGCCCACCTTTTCAATTTTTTATCTTTTTATAATTTCTTTAACTGCAATTAGTTTTTTTTGTATAAAGTTTTCAGCTTTTGATTTTTGGATATGTTGTTGAAGTTTTTTTTTTTTATATATTTTATAGATACAGATATGCTTGTTTTTAAATTATGGCTTAGGGACTGTGGTGGCAGTAGTTGTAGATCTGCTTCTGAGTTTACATTGAGATATGCTTCTTGTGTCCAACCTTCATAACTTAATGGACCAAAATGAAATGAAAAAACTTAAGGGAGCAAATTGTATAATGTTTGTAACTTGAGTGACCAAAGTGAAACAGAACGGATTTAAAGGACCAGAGCAAAACCTAAAAATAACTGAAGGGCCAAAGGTGTAATTTACCCTTGTTGAGTGTGACTTAAAATCTCACCATGTTTCAAGAGAAGGAGGAAAGAATTATGAAAGAGGGTTTTGGTTCTGCCTCGTGCGCTAGGCATGTGAAGTTTGTGTGCTAGGCGCATGGTTTGTGTTTTTTGGCAGAATTTTGATTCTGTCTCATGTGGAGGCGCGCGGCCAGCTGTCAGTTTTGTGCTATACGCGTCCAATCATGTGCTAGGCACGTGTCACGGGTTTTGGACCTCGATTTAAAGATTTTTCCCACTCTTGTGTAATCTCTTGGAACCAAAGAATGCAACTTTTAAGAACTTAGGGCCCGTTTGGTGTGTAGGATATGATAGAGACATGATAGAATAGAGAGTTGGATAAGAATAAGATATGACAGTGATAGGATAGACTTTTTTTCTATCATATGTTTGGATGTACACATGATAAGAAATATGATAGACAGCTAAATGATGATCCTACCCCAACAAACACAATCATACAATCCGACCCGTTATAGACATTTAGAGCTTGAGGGTCTTTGGGTGAGACTCTTAGGTTGGGAAACATTGTAAACACCTCACGTGAGTGAAAATCTTTGAGTTTCTTGTTCATGTGAAGAGATTGGGAAATGAGTAGAAATTAGGTTGTTCTTGGAGAACTTATTGAAGTTAATTACTTGTTTCTCTTTTGTATATCTTTGTAAGAAACTCATTGATAGTGAATTGGAGAGCTCTCTCCCTCAAACATAGGTCATTGTTGGGCCGAACTGGGTAATCAAATTGTAGTCTGTTTGTTCCTCTACTCTCTTTATCTTGTTGCTTTGATTAAGGTTTTGATTTACACTTGGTTGCTAGATCTAGATATGTTGTTGTTGCTTGAGGCTATTTGGTTATTGGTTGCCATTGATACTTGCTCCACAGATTTGAGTCTGAATTCACAACAATTATTCTTTTCCTTGTTCTGCAAGTAGTAAATCATGCACTTTTATTTAAATAAATTGGGGAAGAGGCAAATTTTTATCAATTAAATTGACACTGATAACCATTTTAAATTTGTCCCCGATAAGATTTGAACTCTGTACATTGAGGTTACAAGACCAAGCTCTTACATTAAGCTAGCACCTCAGGTGTATACAAATCGATGTACTTCTTTTTTTATCAATTTGTAAATCATGTACTAGAGATGATTAATAGCTGTTTTTCAAGCAAATAATTATCTTCAGCTCAATGTATCTTTTAATTTCAGATTAAAATTTATATTTTAATTGCAGGCACTTCCTAAAACATTTTCAGACAATTTAAAGAAGAAGTTGCCAGAAAATGTGACTCTTAAAGGTCCTAGTGGAGTTGTGTGGAATATAGGACTGACAACTAGAGATGATACTGTTTACTTCGTGGACAGTTGGCAACAATTCGTGAACGATCATTCTTTGAAAGAGAATGATTTCCTTGTCTTTAAGTACAATGGTGAATCACACTTTGAGGTTTTAATCTTTGATGGAAATAGTTTTTGTGAGAAGGCAACTTCTTATTTTGTCGGAAAATGTGGACATGCTCAAACTGAACAAGGGGATAGCAAGGCAAAGGACAATAACACTTCTGCTTTTAACGCTGGTGTTGAGTCTGCTTCCCCTCAGGTGGCTGATGCAGTCACTAAGACCACACCTGCTGCTGTTCCATCCCAAACAACCAGCAAAAGGACCAAGAAGAAGCCTGTTGTTGAAGTTACGCCTGTCCAGACTAAGAAGCGAGGAAGACCACCAAAATCAGATGATTCTGGTGAGAAGCTACTACGTGATTTGGTGGCTTGTAACAAGGAACACTCAGGTTCAAATATTTCTTCCTTTTTCATTAACCTTTACTATATTTTACAAATTATTTAGTAACTATAACTAATATGTATATCTCTCTCCCTCTCTCTTAACTAACTTGCCAACAAGCACTCTCCTCTGTATTTTGTCCGCTCCCTTAATGTGTCCTATTAAACTCTCTCTCTCAGTCAAGTAATCTCCTGTGACCATTGTATCTTCAACATTACAATCAGTTTTCCTCCTTGTGAATGTTGGAAGAAAAGATTGTGATTTCCTCCTTGTGATTTGATTTCATTCTAGATCTCAATATTAATAACATGTATATCATCTGATCTTCCCTAGCAAACTGATACTTGTCTAGGAAAGAGTATTTTGCATTCAAATCCTTATATTTATGCCAAAGCTTATTATTTTGCGTAAAACTCATGATTGAACTGTTCAATTCCTCACAGAGGCATCCACCCTGGATAGAATCAGGAAGGAAGATGAGAAAAAAATAGCCGAATCATTTACCTCCTCTTTTCCATATTTTGTTAAAATACTTAAAGCCGGCAATGTCGGTGGTTCACGTACTCTG
Protein sequence

>MS.gene063298.t1

ALPKTFSDNLKKKLPENVTLKGPSGVVWNIGLKTRDNTVYFVDGWRQFARDHFLKENDFLVFKYNGESLFNVLTFQGESFCEKAASYFVGKCGNAETEQKDIRENETNKSVEEVNTVLNAGVESASPEKFTANAVSIVLPSQSTGKRTKQPVNEVTPVQTKKRGRPPKKADYSVEREAHDYDLVACNKEHSEASISGKEDEKKIAHSFTSSFPYFVMIMKSCNISGSRTLRIPCHFSATHLPACRTEVTLRNARGECWTVTSIPVGAIRAFCGGWMAFVCDNGINVGDTCTFELISNFVMKVYISGVGKEGLDYQNRDASTLDRIRKEDEKKIAESFTSSFPYFVKILKAGNVGGSRTL