AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24344

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24344.t1 MTR_1g021380 80.063 316 24 2 1 288 36 340 0.0 505
MS.gene24344.t1 MTR_1g021400 63.497 326 73 7 1 289 45 361 1.88e-132 382
MS.gene24344.t1 MTR_1g021400 91.111 45 4 0 287 331 227 271 2.63e-18 85.1
MS.gene24344.t1 MTR_1g021435 59.763 338 78 7 1 289 37 365 2.12e-126 367
MS.gene24344.t1 MTR_1g021410 60.976 328 79 8 1 289 37 354 3.36e-123 358
MS.gene24344.t1 MTR_1g021440 50.681 367 103 8 1 293 37 399 1.68e-112 332
MS.gene24344.t1 MTR_1g021440 48.077 364 99 6 1 293 37 381 3.51e-106 315
MS.gene24344.t1 MTR_1g021330 52.212 339 102 8 1 289 41 369 4.22e-105 313
MS.gene24344.t1 MTR_1g021360 51.875 320 84 7 1 288 197 478 1.07e-96 295
MS.gene24344.t1 MTR_1g021360 70.543 129 38 0 1 129 40 168 7.21e-56 189
MS.gene24344.t1 MTR_1g021440 58.209 268 74 5 1 234 37 300 1.80e-96 288
MS.gene24344.t1 MTR_1g021320 43.427 426 106 9 1 295 197 618 2.98e-92 293
MS.gene24344.t1 MTR_1g021320 72.093 129 36 0 1 129 40 168 1.91e-56 196
MS.gene24344.t1 MTR_1g021320 30.931 333 168 12 2 290 523 837 1.11e-28 117
MS.gene24344.t1 MTR_1g021440 55.172 261 67 3 1 230 37 278 4.92e-90 271
MS.gene24344.t1 MTR_1g021350 50.962 312 115 10 1 289 36 332 1.17e-89 272
MS.gene24344.t1 MTR_1g021290 56.316 190 69 3 1 190 170 345 6.40e-58 191
MS.gene24344.t1 MTR_1g021290 76.289 97 23 0 1 97 42 138 2.45e-46 160
MS.gene24344.t1 MTR_1g021270 33.923 339 164 10 2 289 31 360 2.95e-43 157
MS.gene24344.t1 MTR_1g021270 29.882 338 171 10 2 291 272 591 1.76e-28 115
MS.gene24344.t1 MTR_1g021500 43.318 217 59 6 1 171 41 239 1.06e-41 150
MS.gene24344.t1 MTR_1g021500 43.318 217 59 6 1 171 41 239 1.97e-41 150
MS.gene24344.t1 MTR_7g050710 25.000 300 186 10 2 282 29 308 5.34e-14 72.0
MS.gene24344.t1 MTR_3g098790 24.497 298 173 12 2 276 29 297 6.65e-14 72.0
MS.gene24344.t1 MTR_7g050640 37.333 75 47 0 2 76 29 103 4.68e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24344.t1 AT4G33280 31.579 285 155 11 1 265 38 302 4.84e-30 117
MS.gene24344.t1 AT4G33280 27.964 329 170 9 1 265 38 363 6.18e-29 115
MS.gene24344.t1 AT4G33280 50.459 109 53 1 1 108 38 146 7.05e-29 112
MS.gene24344.t1 AT3G18990 27.500 320 176 16 2 274 22 332 6.87e-17 80.9
MS.gene24344.t1 AT3G18990 27.500 320 176 16 2 274 22 332 9.33e-17 80.5
MS.gene24344.t1 AT4G34400 35.294 85 55 0 1 85 30 114 1.41e-15 77.4
MS.gene24344.t1 AT4G34400 35.294 85 55 0 1 85 30 114 1.41e-15 77.4
MS.gene24344.t1 AT5G60130 37.500 88 50 2 1 83 28 115 2.02e-11 63.5
MS.gene24344.t1 AT5G60130 37.500 88 50 2 1 83 2 89 2.57e-11 63.9
MS.gene24344.t1 AT5G60130 37.500 88 50 2 1 83 28 115 3.49e-11 63.5
MS.gene24344.t1 AT5G60130 37.500 88 50 2 1 83 25 112 3.52e-11 63.9
MS.gene24344.t1 AT5G60130 37.500 88 50 2 1 83 28 115 3.54e-11 63.9

Find 0 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
8662 gene 7420 15186 7420 ID=MS.gene24344
8662 mRNA 7420 15186 7420 ID=MS.gene24344.t1;Parent=MS.gene24344
8662 exon 14613 15186 14613 ID=MS.gene24344.t1.exon1;Parent=MS.gene24344.t1
8662 CDS 14613 15186 14613 ID=cds.MS.gene24344.t1;Parent=MS.gene24344.t1
8662 exon 14130 14245 14130 ID=MS.gene24344.t1.exon2;Parent=MS.gene24344.t1
8662 CDS 14130 14245 14130 ID=cds.MS.gene24344.t1;Parent=MS.gene24344.t1
8662 exon 13714 13893 13714 ID=MS.gene24344.t1.exon3;Parent=MS.gene24344.t1
8662 CDS 13714 13893 13714 ID=cds.MS.gene24344.t1;Parent=MS.gene24344.t1
8662 exon 7420 7542 7420 ID=MS.gene24344.t1.exon4;Parent=MS.gene24344.t1
8662 CDS 7420 7542 7420 ID=cds.MS.gene24344.t1;Parent=MS.gene24344.t1
Gene Sequence

>MS.gene24344

GCACTTCCTAAAACATTCTCAGACAATTTAAAGAAGAAGTTGCCAGAAAATGTGACCCTTAAAGGTCCTAGTGGAGTTGTGTGGAATATAGGGCTGAAAACTAGAGATAATACTGTTTACTTCGTGGACGGTTGGCGTCAGTTTGCAAGAGATCATTTTCTGAAAGAGAATGATTTCCTGGTCTTTAAGTACAATGGTGAATCACTATTCAACGTTTTGACCTTTCAAGGAGAAAGCTTTTGTGAGAAAGCGGCTTCCTATTTTGTTGGAAAATGTGGGAATGCTGAAACTGAACAAAAGGACATCAGGGAGAACGAAACAAACAAGTCTGTTGAAGAAGTCAATACTGTTCTTAATGCTGGTGTTGAGTCTGCTTCCCCTGAGAAGTTCACGGCTAATGCAGTCAGTATAGTGCTTCCCTCCCAATCAACCGGAAAAAGAACCAAGCAGCCTGTTAATGAGGTTACACCTGTCCAGACTAAGAAGCGAGGAAGACCACCGAAAAAAGCTGATTATTCTGTTGAGAGAGAAGCGCATGATTATGATTTGGTGGCTTGCAACAAGGAACACTCAGGTTAAAATATTTATTTATTTTTCATTAACTTTTACTATCTTGATTTTGGCTTTTCCCTTAATGGGTCCTTTTATACTCTTTCTCAGTCATGTAATCTCATGTAACCATAATATAATCAACATAAACAAAAAATACAATTAGTTTTCCTCCTTGTGAATATTTGAAGAAAAGATTGTGATTTTCTTGACTTTGCTCATAGCCCTTTGATTTCATTCTAGGTCTCAAAACTATGGATTAAAACTCATCTGATCTTCCCTAGCAAACTGATACTTGTCTAGGAAAGAGTATTTTGCATTCAAATCCTTATATTTGTGTCAGAGCTTATTATTTTGTTTAAAATTCATGATTGAACTGTTCAATTCCACAGAGGCATCCATCAGTGGGAAGGAAGATGAGAAAAAGATAGCCCACTCATTTACCTCCTCTTTTCCATATTTTGTTATGATAATGAAATCCTGCAATATCAGTGGTTCACGTACTCTGGTGAGTTCTTCCCCTTTGTGATGCTTTATTTTACTCCCAAATAATGTTGTTTTATCTCTCCACCCTTATCTTTAAGTAACTTATGTAGTGTTTCATCTGCTTGAGAAACTAATCCAGTTGGAGTATAATATTGAATACTTGTTATATTGTAGAGAATCCCATGTCATTTCTCTGCTACACATCTCCCTGCCTGCAGAACAGAAGTTACGCTTCGAAATGCAAGAGGCGAATGTTAGACTGTGACCTCCATCCCAGTGGGAGCAACACGCGCCTTTTGTGGGGGATGGATGGCCTTTGTTCGTGATAATGGCATCAATGTTGGCGACACATGCACTTTTGAGCTCGTTTCTAATTTTGTGATGAAGGTTTATATATCTGGGGTTGGAAAAGAAGGTCTTGACTATCAAAATGGAGATGTGAAACTTAATAGCTAGTTACATCTACCTGCCAATGTGCTTGTTTGTGAGACAGATAAATCAATCTTAGATGAAGTTTTTTTGTGGATGAGTTGCCAAGACTTGATACATTTTATAGATGATGATTTTTTGTGGCATGAGTTGCCAAGACTTGATACATTTTATAGATGATGATTTTTTGTTACGCTGCTGCATTTTAGTTCATGTCATTGCGAAATGTTGGTGATACGACTCAATTCGAGAAGGGATCACACTTCTGAATTTAGGGAGAGAAGTTAGAACGAAAAAGGGGAATTTGATTATATTTTTCATTGATTTTAATCATGAGACAATACAAATGCTTATGTAAGCTAGTGCATACTCATAGTAAGATAACTACATCAATCACACAATGTCACTATTACCAAACAAACCAATAAAGTATGCATAAAAGATCCCTTCTAGAATAAATAATTATGAGTTATCATTCTCTTATTTTAAGGGTTGTAAAAAAATGGTCTCAACACATCATAGACCTCAAAAGCTACGTGCACATATTCTTAGATTTATACAAATCATTGCATCATTGTATGCAAGGCAATTTACTGCATCATTGTATGCAAGGGAAGCTCTATCTGGGAGCATTTTTTTCCAGCTAATGTGTTTCCAAGCGTCACACCATACGGCCAAACTTTGTCACACTCACCACCCCACAATTGCCAAACAATAAAAATATCTCAAACCATTATCATTTGCAACCCAATTAGCATTGAACTATCTGCCTTAAATCCTTGAAAACTATCTGTTTTGCATAGTGTTGACTCACAGTTTCTCAAGCAAAGCAACAGTCTTAGGATCAACAAGATGAAAGGAAGTTGAGCTCCTTCGCCTTTCACGGTAGTCACTGCCCATGCAGCTAAAAACACTTTCTTCCTGATTGTAGCCTTGGATAAAGAAATGGACGTATAGTATTGAAGGTGTATATAGTTGGTTCGTGGAAATACTTACCTTTACAGTGCCATGCTTGAACACATAATTCACACTTTTCATCACATTAAAAACAAGATGCTCCATGTAGCCAAATACATGAAAATCTTTACTTATAAATTCAATCGAACTCGTCAAATTAGCAATGCTTGAGAAACCAAGGGCAGTACTCACAATAGCTCTTGCCCTCATTCACTTGCCTTCCTTTGTTGTGATCCAAAAGAACTTGTCTGCAACCTCATCCACACAATTACCAAGAGTAGCACATTGGTCTCCCTCTAGTGATAGTTGAACTTATCTGCAAGGTTCGGATTATCAAGTCTAACTTCATTAATGACACAAAAACTTGGCTAGTGTACCAAAACTCCATTGTTTCAACAACATCAGCAATTGATTGAACCTCCGTAGTAGTTGAAGTTGTACCCTCTCTGAACTGTGATTGCACACGTTGATCATTTTCCATTGCCTCCATCGTTGGAACTTTTCGCATCATAAAGCAATAATATTGGTGAGATGAATTGTCCTCAAGTATAGTATACAATCAACAGACTATAGAGGAAGAGAAGCGCTAAAATTGAAAGAGAATTTGAAAATGAGCAAGAGGAATTAGTTTTCTTTATTTTGTTCTAACTTAGTAGGATAACATTTTGACGGAATAACCTATTTCGTCCTCAAATTTGTACGTATGGTCATTTTTATCATCTAATTTTTCGAAATAGTGGATATGTCCATGAATTTTATAAATGTCAATCAATTTAGTCCCTTTTACAAGTCTTGGGTTAACAGTGTTGACGTGTCCCATTAAACGCTAACGTGGAGTTCCAAGTCGGCTTCCACGAGTATTGGGACGAATTTGATCGAAAAAAATTTAAAATTAATTTTTGTACTTTTTTTTAAATGAAAGTCATTGTAAATTGTTACGATGTCCAATCGATCCATGAATTATTCATTAAAAATCAAGTTGGTCCCTAAATTATGTTACTCGCTATCAAACCGATCCCTGAATTATTTATCTTATCTATTAATATAATCCTTGGTACTATCAAATAAATGTTAAAAACTAAAATAATGTAAAGAATGTGTTTAACCGTTGTTTATTACCTTTTTTCATGTTGAAATATTACATGACCATCTTATTTAAACTCGACCTTTAGCAAATTAACACCTACAAAATTTAAAAATCTTGGCATACAGTTTAGCAGCAGAACCAACTATTCAGGCGTAGATGCAAAGTGTAACACAAAGAATTGATCCTAAAGCTTTATGAGTATTGGATAAGTCGTTGACAAATATATAAACTCCTAAAAAATGATACTCTAGCAAGCTCCAAACAGTTAAAAAGTCTCTGTGATATTTAAATGATATTTTCAAGAAGATGGATGTAAATGTTCTCTCTCCTGATCGATCAGGCCTTTCAGCTTTAACAACTTGTTACAAGAAATGTTTTGGCAGCGGAAAAACTTGTATTTGAAGAACTGTTGTGAAGAAATTCATGACATGGAGATCATTAATTTTCTAATATGAAAACTCAAACCACTTGTTAATTTCAAACACTAGAGGATAAATTATATCATCATAAATATAAAAGTCAAATAATTCTAAAACCCTACAAACTATTAACCACCATGGAAAGCTTTAATTTAAGATCGAATACTACACTAGGTATTTGTTTAGCACAAACCAAACTTATGCAACAAGATAATAAGCTCAAATCAGTATGATAGTTCATAAAAGTTCAAATCTTTCTGGAGAGCAGATAATTTTCAAGAGTACATAAGAAGAAACTGGTGTGATAAAAATAGCATGCAAATGAAATTATTGCAAAGAGAAATAAGTAGAGTGAAAAGAAGGTTATAACTATCTAATAATAATACATAGAAATTTGAGGATGAAATAGTTTATTCACTCATAATTTTTTTGTGCAACATGTCAAAAAGTTATAGGTGGATAACTTATTACTAAATTTAACATATATAAGATTATTTCTATAAAATAATAATGTGTTCAAATTAAATAATATCTTTCATTTAAAATATTATTTTTGAATAAAATATAAACAAAAATTATACATTTTCATATGACTATTACAAGTAAAAATAAACATTTTACATTCATTCATTAAATAATATATGTGACTTATAATAAAGATTATATACATCGTTAATTGATTGAATTTAAGATGTTTATAATAGTGACTGGAAGAGGTAGTTAAAAAGATAAAGTTGAACTAGCCTTTCACTTCCTCGTGTACTGTTCAAGGATTAAAGCAGCCTGGAACTTTCTTTCTTGCTCGTACCCTTTCTTTCATGCTAAAACAAACTGACTGTTTCAAATGGGAGGAAGAGAAGGAGGACAAAACTGGGATGGAAGCAGATCTTGGGAGGAAGATATTTACTGGAACCATTTTCAATTCATCCATTTCACCCTATTTCTTAAAACTACCACCAATTTTCAACAACAACTTGTAAGTACTTCTTCTTTTCCTTCCATCTTCTAAAGTATTCATATTTGGGTGTTCACTGTGGAGGCAGTACATACATGCACTTCTTCCATGTACTATTTTTCATTGTTTTTACTTGCCCACCTTTTCAATTTTTTATCTTTTTATAATTTCTTTAACTGCAATTAGTTTTTTTTGTATAAAGTTTTCAGCTTTTGATTTTTGGATATGTTGTTGAAGTTTTTTTTTTTTATATATTTTATAGATACAGATATGCTTGTTTTTAAATTATGGCTTAGGGACTGTGGTGGCAGTAGTTGTAGATCTGCTTCTGAGTTTACATTGAGATATGCTTCTTGTGTCCAACCTTCATAACTTAATGGACCAAAATGAAATGAAAAAACTTAAGGGAGCAAATTGTATAATGTTTGTAACTTGAGTGACCAAAGTGAAACAGAACGGATTTAAAGGACCAGAGCAAAACCTAAAAATAACTGAAGGGCCAAAGGTGTAATTTACCCTTGTTGAGTGTGACTTAAAATCTCACCATGTTTCAAGAGAAGGAGGAAAGAATTATGAAAGAGGGTTTTGGTTCTGCCTCGTGCGCTAGGCATGTGAAGTTTGTGTGCTAGGCGCATGGTTTGTGTTTTTTGGCAGAATTTTGATTCTGTCTCATGTGGAGGCGCGCGGCCAGCTGTCAGTTTTGTGCTATACGCGTCCAATCATGTGCTAGGCACGTGTCACGGGTTTTGGACCTCGATTTAAAGATTTTTCCCACTCTTGTGTAATCTCTTGGAACCAAAGAATGCAACTTTTAAGAACTTAGGGCCCGTTTGGTGTGTAGGATATGATAGAGACATGATAGAATAGAGAGTTGGATAAGAATAAGATATGACAGTGATAGGATAGACTTTTTTTCTATCATATGTTTGGATGTACACATGATAAGAAATATGATAGACAGCTAAATGATGATCCTACCCCAACAAACACAATCATACAATCCGACCCGTTATAGACATTTAGAGCTTGAGGGTCTTTGGGTGAGACTCTTAGGTTGGGAAACATTGTAAACACCTCACGTGAGTGAAAATCTTTGAGTTTCTTGTTCATGTGAAGAGATTGGGAAATGAGTAGAAATTAGGTTGTTCTTGGAGAACTTATTGAAGTTAATTACTTGTTTCTCTTTTGTATATCTTTGTAAGAAACTCATTGATAGTGAATTGGAGAGCTCTCTCCCTCAAACATAGGTCATTGTTGGGCCGAACTGGGTAATCAAATTGTAGTCTGTTTGTTCCTCTACTCTCTTTATCTTGTTGCTTTGATTAAGGTTTTGATTTACACTTGGTTGCTAGATCTAGATATGTTGTTGTTGCTTGAGGCTATTTGGTTATTGGTTGCCATTGATACTTGCTCCACAGATTTGAGTCTGAATTCACAACAATTATTCTTTTCCTTGTTCTGCAAGTAGTAAATCATGCACTTTTATTTAAATAAATTGGGGAAGAGGCAAATTTTTATCAATTAAATTGACACTGATAACCATTTTAAATTTGTCCCCGATAAGATTTGAACTCTGTACATTGAGGTTACAAGACCAAGCTCTTACATTAAGCTAGCACCTCAGGTGTATACAAATCGATGTACTTCTTTTTTTATCAATTTGTAAATCATGTACTAGAGATGATTAATAGCTGTTTTTCAAGCAAATAATTATCTTCAGCTCAATGTATCTTTTAATTTCAGATTAAAATTTATATTTTAATTGCAGGCACTTCCTAAAACATTTTCAGACAATTTAAAGAAGAAGTTGCCAGAAAATGTGACTCTTAAAGGTCCTAGTGGAGTTGTGTGGAATATAGGACTGACAACTAGAGATGATACTGTTTACTTCGTGGACAGTTGGCAACAATTCGTGAACGATCATTCTTTGAAAGAGAATGATTTCCTTGTCTTTAAGTACAATGGTGAATCACACTTTGAGGTTTTAATCTTTGATGGAAATAGTTTTTGTGAGAAGGCAACTTCTTATTTTGTCGGAAAATGTGGACATGCTCAAACTGAACAAGGGGATAGCAAGGCAAAGGACAATAACACTTCTGCTTTTAACGCTGGTGTTGAGTCTGCTTCCCCTCAGGTGGCTGATGCAGTCACTAAGACCACACCTGCTGCTGTTCCATCCCAAACAACCAGCAAAAGGACCAAGAAGAAGCCTGTTGTTGAAGTTACGCCTGTCCAGACTAAGAAGCGAGGAAGACCACCAAAATCAGATGATTCTGGTGAGAAGCTACTACGTGATTTGGTGGCTTGTAACAAGGAACACTCAGGTTCAAATATTTCTTCCTTTTTCATTAACCTTTACTATATTTTACAAATTATTTAGTAACTATAACTAATATGTATATCTCTCTCCCTCTCTCTTAACTAACTTGCCAACAAGCACTCTCCTCTGTATTTTGTCCGCTCCCTTAATGTGTCCTATTAAACTCTCTCTCTCAGTCAAGTAATCTCCTGTGACCATTGTATCTTCAACATTACAATCAGTTTTCCTCCTTGTGAATGTTGGAAGAAAAGATTGTGATTTCCTCCTTGTGATTTGATTTCATTCTAGATCTCAATATTAATAACATGTATATCATCTGATCTTCCCTAGCAAACTGATACTTGTCTAGGAAAGAGTATTTTGCATTCAAATCCTTATATTTATGCCAAAGCTTATTATTTTGCGTAAAACTCATGATTGAACTGTTCAATTCCTCACAGAGGCATCCACCCTGGATAGAATCAGGAAGGAAGATGAGAAAAAAATAGCCGAATCATTTACCTCCTCTTTTCCATATTTTGTTAAAATACTTAAAGCCGGCAATGTCGGTGGTTCACGTACTCTG
Protein sequence

>MS.gene24344.t1

ALPKTFSDNLKKKLPENVTLKGPSGVVWNIGLKTRDNTVYFVDGWRQFARDHFLKENDFLVFKYNGESLFNVLTFQGESFCEKAASYFVGKCGNAETEQKDIRENETNKSVEEVNTVLNAGVESASPEKFTANAVSIVLPSQSTGKRTKQPVNEVTPVQTKKRGRPPKKADYSVEREAHDYDLVACNKEHSEASISGKEDEKKIAHSFTSSFPYFVMIMKSCNISGSRTLTVTSIPVGATRAFCGGWMAFVRDNGINVGDTCTFELVSNFVMKVYISGVGKEGLDYQNGDASTLDRIRKEDEKKIAESFTSSFPYFVKILKAGNVGGSRTL