Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064890.t1 | XP_003603905.1 | 89.2 | 74 | 8 | 0 | 1 | 74 | 33 | 106 | 2.30E-29 | 139.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064890.t1 | G7J6J7 | 89.2 | 74 | 8 | 0 | 1 | 74 | 33 | 106 | 1.6e-29 | 139.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04921 | MS.gene064890 | 0.802321 | 5.93E-49 | -1.69E-46 |
| MS.gene050338 | MS.gene064890 | 0.82045 | 6.82E-53 | -1.69E-46 |
| MS.gene052702 | MS.gene064890 | 0.80433 | 2.28E-49 | -1.69E-46 |
| MS.gene056694 | MS.gene064890 | 0.831816 | 1.35E-55 | -1.69E-46 |
| MS.gene056697 | MS.gene064890 | 0.83367 | 4.67E-56 | -1.69E-46 |
| MS.gene058334 | MS.gene064890 | 0.807684 | 4.48E-50 | -1.69E-46 |
| MS.gene058382 | MS.gene064890 | 0.844859 | 5.82E-59 | -1.69E-46 |
| MS.gene05926 | MS.gene064890 | 0.821492 | 3.92E-53 | -1.69E-46 |
| MS.gene061044 | MS.gene064890 | 0.832772 | 7.81E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064890.t1 | MTR_3g116440 | 84.985 | 333 | 41 | 3 | 1 | 330 | 33 | 359 | 0.0 | 520 |
| MS.gene064890.t1 | MTR_3g034610 | 64.489 | 352 | 102 | 3 | 1 | 329 | 1 | 352 | 3.76e-143 | 410 |
| MS.gene064890.t1 | MTR_3g034610 | 69.258 | 283 | 81 | 2 | 53 | 329 | 122 | 404 | 1.49e-129 | 376 |
| MS.gene064890.t1 | MTR_3g034640 | 51.813 | 386 | 122 | 5 | 1 | 328 | 33 | 412 | 1.39e-113 | 338 |
| MS.gene064890.t1 | MTR_3g034640 | 60.638 | 282 | 105 | 2 | 53 | 328 | 209 | 490 | 8.10e-108 | 323 |
| MS.gene064890.t1 | MTR_3g034640 | 62.222 | 270 | 96 | 2 | 65 | 328 | 169 | 438 | 7.30e-107 | 321 |
| MS.gene064890.t1 | MTR_3g034640 | 60.227 | 264 | 103 | 1 | 47 | 308 | 229 | 492 | 2.71e-100 | 304 |
| MS.gene064890.t1 | MTR_3g034640 | 54.639 | 97 | 40 | 1 | 237 | 329 | 57 | 153 | 1.79e-20 | 92.4 |
| MS.gene064890.t1 | MTR_3g116430 | 56.125 | 351 | 127 | 6 | 1 | 328 | 33 | 379 | 1.12e-112 | 333 |
| MS.gene064890.t1 | MTR_3g116430 | 58.077 | 260 | 106 | 2 | 47 | 305 | 173 | 430 | 2.27e-89 | 274 |
| MS.gene064890.t1 | MTR_3g107810 | 49.102 | 334 | 148 | 4 | 1 | 328 | 33 | 350 | 8.84e-93 | 284 |
| MS.gene064890.t1 | MTR_3g107810 | 49.286 | 280 | 140 | 2 | 53 | 330 | 199 | 478 | 6.97e-80 | 251 |
| MS.gene064890.t1 | MTR_4g069810 | 35.440 | 364 | 180 | 17 | 1 | 328 | 34 | 378 | 4.80e-43 | 152 |
| MS.gene064890.t1 | MTR_4g069810 | 37.799 | 209 | 120 | 7 | 127 | 330 | 77 | 280 | 6.56e-32 | 123 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 63 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTGGTTCAAAATCTTTAT+TGG | 0.220637 | 3.3:-94850904 | None:intergenic |
| TCTTGGTTCAAAATCTTTAT+TGG | 0.225126 | 3.3:-94850982 | None:intergenic |
| TCTTGGTTCAAAATCTTTAT+TGG | 0.225126 | 3.3:-94851060 | None:intergenic |
| AGACCTAACATTTCAGCTTA+TGG | 0.239620 | 3.3:+94851236 | MS.gene064890:CDS |
| AGACCCAATGTTTCAGCTTA+TGG | 0.248284 | 3.3:+94850690 | MS.gene064890:CDS |
| AGACCCAATGTTTCAGCTTA+TGG | 0.248284 | 3.3:+94850846 | MS.gene064890:CDS |
| AGACCCAATGTTTCAGCTTA+TGG | 0.248284 | 3.3:+94851002 | MS.gene064890:CDS |
| AGACCTAATGTTTCAGCTTA+TGG | 0.249885 | 3.3:+94850612 | MS.gene064890:CDS |
| GACCTAACATTTCAGCTTAT+GGG | 0.310475 | 3.3:+94851237 | MS.gene064890:CDS |
| AGGTCTTGGTTCAAAATCTT+TGG | 0.310679 | 3.3:-94851219 | None:intergenic |
| CATTTATGCATCATATTTAG+TGG | 0.320826 | 3.3:-94851312 | None:intergenic |
| GTTCCATAAGCTGAAACATT+AGG | 0.327800 | 3.3:-94850615 | None:intergenic |
| AAACAATTACATTGATGTTA+TGG | 0.330758 | 3.3:+94851181 | MS.gene064890:CDS |
| ATATTTAGTGGCACTTGGTC+GGG | 0.347002 | 3.3:-94851300 | None:intergenic |
| AGACAATCGTGTTGAAGTTA+AGG | 0.379767 | 3.3:+94850791 | MS.gene064890:CDS |
| AGACAATGGTATTGATGTTA+AGG | 0.392683 | 3.3:+94850869 | MS.gene064890:CDS |
| AGACAATGGTATTGATGTTA+AGG | 0.392683 | 3.3:+94851025 | MS.gene064890:CDS |
| ATTGATGTTAAGGAAAATAA+AGG | 0.395870 | 3.3:+94850879 | MS.gene064890:CDS |
| ATTGATGTTAAGGAAAATAA+AGG | 0.395870 | 3.3:+94851035 | MS.gene064890:CDS |
| AGACCCAATGTTTCAGCATA+TGG | 0.405735 | 3.3:+94851080 | MS.gene064890:CDS |
| ATGAATGATGTCATTGTCTA+TGG | 0.405969 | 3.3:-94850478 | None:intergenic |
| ATTGATGTTACAGAAAATAA+AGG | 0.415243 | 3.3:+94850957 | MS.gene064890:CDS |
| ACACCCAATGTTTCAGCATA+TGG | 0.418154 | 3.3:+94850768 | MS.gene064890:CDS |
| TCTCCATATGCTGAAATGTT+TGG | 0.423109 | 3.3:-94850927 | None:intergenic |
| TATGCTGAAACATTGGGTCT+TGG | 0.433141 | 3.3:-94851155 | None:intergenic |
| TAAGCTGAAACATTGGGTCT+TGG | 0.435355 | 3.3:-94850843 | None:intergenic |
| TATGCTGAAATGTTTGGTCT+TGG | 0.439257 | 3.3:-94850921 | None:intergenic |
| GACAATCGTGTTGAAGTTAA+GGG | 0.442091 | 3.3:+94850792 | MS.gene064890:CDS |
| AGACCAAACATTTCAGCATA+TGG | 0.445583 | 3.3:+94850924 | MS.gene064890:CDS |
| CATATTTAGTGGCACTTGGT+CGG | 0.446372 | 3.3:-94851301 | None:intergenic |
| GTCTCCATATGCTGAAACAT+TGG | 0.449066 | 3.3:-94851084 | None:intergenic |
| TAAGCTGAAATGTTAGGTCT+TGG | 0.458122 | 3.3:-94851233 | None:intergenic |
| AAACCTTTGAAAGACTGCTT+CGG | 0.462349 | 3.3:-94850411 | None:intergenic |
| TAAGCTGAAACATTAGGTCT+TGG | 0.463292 | 3.3:-94850609 | None:intergenic |
| TAAGCTGAAACATTGGGTCT+TGG | 0.464372 | 3.3:-94850687 | None:intergenic |
| TAAGCTGAAACATTGGGTCT+TGG | 0.464372 | 3.3:-94850999 | None:intergenic |
| TATGAAGATAATAACATTGA+GGG | 0.464536 | 3.3:+94850558 | MS.gene064890:CDS |
| ATTCATGCTAAGAGAAAGAA+AGG | 0.472696 | 3.3:+94850495 | MS.gene064890:CDS |
| TATGCTGAAACATTGGGTCT+TGG | 0.475638 | 3.3:-94851077 | None:intergenic |
| TCTCCATATGCTGAAACATT+GGG | 0.476305 | 3.3:-94851083 | None:intergenic |
| AACAAATGAAATTGATGCTA+AGG | 0.478202 | 3.3:+94850635 | MS.gene064890:CDS |
| GTCTCCATAAGCTGAAACAT+TGG | 0.487144 | 3.3:-94850694 | None:intergenic |
| GTCTCCATAAGCTGAAACAT+TGG | 0.487144 | 3.3:-94850850 | None:intergenic |
| GTCTCCATAAGCTGAAACAT+TGG | 0.487144 | 3.3:-94851006 | None:intergenic |
| TCCCCATAAGCTGAAATGTT+AGG | 0.487972 | 3.3:-94851239 | None:intergenic |
| TATTTAGTGGCACTTGGTCG+GGG | 0.498218 | 3.3:-94851299 | None:intergenic |
| TCTCCATAAGCTGAAACATT+GGG | 0.499953 | 3.3:-94850693 | None:intergenic |
| TCTCCATAAGCTGAAACATT+GGG | 0.499953 | 3.3:-94850849 | None:intergenic |
| TCTCCATAAGCTGAAACATT+GGG | 0.499953 | 3.3:-94851005 | None:intergenic |
| GTCTCCATATGCTGAAACAT+TGG | 0.510420 | 3.3:-94850772 | None:intergenic |
| GCATCATATTTAGTGGCACT+TGG | 0.527772 | 3.3:-94851305 | None:intergenic |
| AATTGATGCTAAGGAAAAGA+AGG | 0.533314 | 3.3:+94850644 | MS.gene064890:CDS |
| ACTCCGAAGCAGTCTTTCAA+AGG | 0.542773 | 3.3:+94850408 | MS.gene064890:CDS |
| TCTCCATATGCTGAAACATT+GGG | 0.551421 | 3.3:-94850771 | None:intergenic |
| ACCTAACATTTCAGCTTATG+GGG | 0.562671 | 3.3:+94851238 | MS.gene064890:CDS |
| GACATGCGAAAAGAAATTCA+AGG | 0.567894 | 3.3:+94850351 | MS.gene064890:CDS |
| GTTTCAGCTTATGGAGACAA+TGG | 0.577650 | 3.3:+94850855 | MS.gene064890:CDS |
| GTTTCAGCTTATGGAGACAA+TGG | 0.577650 | 3.3:+94851011 | MS.gene064890:CDS |
| ATATGAAGATAATAACATTG+AGG | 0.587671 | 3.3:+94850557 | MS.gene064890:CDS |
| TATGCTGAAACATTGGGTGT+TGG | 0.621863 | 3.3:-94850765 | None:intergenic |
| ACATGCGAAAAGAAATTCAA+GGG | 0.625015 | 3.3:+94850352 | MS.gene064890:CDS |
| TATTGATGTTGAATTCACAA+AGG | 0.628277 | 3.3:+94851268 | MS.gene064890:CDS |
| ACTTGTGTGTCTATTACAAG+TGG | 0.633990 | 3.3:-94850453 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACAATTACATTGATGTTA+TGG | + | chr3.3:94851181-94851200 | MS.gene064890:CDS | 20.0% |
| !! | ATATGAAGATAATAACATTG+AGG | + | chr3.3:94850557-94850576 | MS.gene064890:CDS | 20.0% |
| !! | ATTGATGTTAAGGAAAATAA+AGG | + | chr3.3:94850879-94850898 | MS.gene064890:CDS | 20.0% |
| !! | ATTGATGTTACAGAAAATAA+AGG | + | chr3.3:94850957-94850976 | MS.gene064890:CDS | 20.0% |
| !! | TATGAAGATAATAACATTGA+GGG | + | chr3.3:94850558-94850577 | MS.gene064890:CDS | 20.0% |
| !!! | ATCTTCATATTTTGAAACAT+TGG | - | chr3.3:94850547-94850566 | None:intergenic | 20.0% |
| !!! | TCTTCATATTTTGAAACATT+GGG | - | chr3.3:94850546-94850565 | None:intergenic | 20.0% |
| ! | AACAAATGAAATTGATGCTA+AGG | + | chr3.3:94850635-94850654 | MS.gene064890:CDS | 25.0% |
| ! | TATTGATGCTAAGAAAAAGA+AGG | + | chr3.3:94850722-94850741 | MS.gene064890:CDS | 25.0% |
| ! | TATTGATGTTGAATTCACAA+AGG | + | chr3.3:94851268-94851287 | MS.gene064890:CDS | 25.0% |
| ! | TCTTGGTTCAAAATCTTTAT+TGG | - | chr3.3:94850907-94850926 | None:intergenic | 25.0% |
| !! | TTTTCATATGCTGAAACATT+GGG | - | chr3.3:94851164-94851183 | None:intergenic | 25.0% |
| AATTGATGCTAAGGAAAAGA+AGG | + | chr3.3:94850644-94850663 | MS.gene064890:CDS | 30.0% | |
| ACATGCGAAAAGAAATTCAA+GGG | + | chr3.3:94850352-94850371 | MS.gene064890:CDS | 30.0% | |
| ATGAATGATGTCATTGTCTA+TGG | - | chr3.3:94850481-94850500 | None:intergenic | 30.0% | |
| ATTCATGCTAAGAGAAAGAA+AGG | + | chr3.3:94850495-94850514 | MS.gene064890:CDS | 30.0% | |
| ! | GTTTTCATATGCTGAAACAT+TGG | - | chr3.3:94851165-94851184 | None:intergenic | 30.0% |
| !! | AGACAATGGTATTGATGTTA+AGG | + | chr3.3:94850869-94850888 | MS.gene064890:CDS | 30.0% |
| !!! | TATTTTGAAACATTGGGTCT+TGG | - | chr3.3:94850540-94850559 | None:intergenic | 30.0% |
| ACCTAACATTTCAGCTTATG+GGG | + | chr3.3:94851238-94851257 | MS.gene064890:CDS | 35.0% | |
| ACTTGTGTGTCTATTACAAG+TGG | - | chr3.3:94850456-94850475 | None:intergenic | 35.0% | |
| AGACAATCGTGTTGAAGTTA+AGG | + | chr3.3:94850791-94850810 | MS.gene064890:CDS | 35.0% | |
| AGACCAAACATTTCAGCATA+TGG | + | chr3.3:94850924-94850943 | MS.gene064890:CDS | 35.0% | |
| AGACCTAACATTTCAGCTTA+TGG | + | chr3.3:94851236-94851255 | MS.gene064890:CDS | 35.0% | |
| AGACCTAATGTTTCAGCTTA+TGG | + | chr3.3:94850612-94850631 | MS.gene064890:CDS | 35.0% | |
| AGGTCTTGGTTCAAAATCTT+TGG | - | chr3.3:94851222-94851241 | None:intergenic | 35.0% | |
| GACAATCGTGTTGAAGTTAA+GGG | + | chr3.3:94850792-94850811 | MS.gene064890:CDS | 35.0% | |
| GACATGCGAAAAGAAATTCA+AGG | + | chr3.3:94850351-94850370 | MS.gene064890:CDS | 35.0% | |
| GACCTAACATTTCAGCTTAT+GGG | + | chr3.3:94851237-94851256 | MS.gene064890:CDS | 35.0% | |
| GTTCCATAAGCTGAAACATT+AGG | - | chr3.3:94850618-94850637 | None:intergenic | 35.0% | |
| TAAGCTGAAACATTAGGTCT+TGG | - | chr3.3:94850612-94850631 | None:intergenic | 35.0% | |
| TAAGCTGAAATGTTAGGTCT+TGG | - | chr3.3:94851236-94851255 | None:intergenic | 35.0% | |
| TATGCTGAAATGTTTGGTCT+TGG | - | chr3.3:94850924-94850943 | None:intergenic | 35.0% | |
| TCTCCATAAGCTGAAACATT+GGG | - | chr3.3:94850696-94850715 | None:intergenic | 35.0% | |
| TCTCCATATGCTGAAACATT+GGG | - | chr3.3:94850774-94850793 | None:intergenic | 35.0% | |
| TCTCCATATGCTGAAATGTT+TGG | - | chr3.3:94850930-94850949 | None:intergenic | 35.0% | |
| ! | AAACCTTTGAAAGACTGCTT+CGG | - | chr3.3:94850414-94850433 | None:intergenic | 35.0% |
| !! | CTTGAGATTCTTTTTCGTCT+CGG | - | chr3.3:94850388-94850407 | None:intergenic | 35.0% |
| ACACCCAATGTTTCAGCATA+TGG | + | chr3.3:94850768-94850787 | MS.gene064890:CDS | 40.0% | |
| AGACCCAATGTTTCAGCATA+TGG | + | chr3.3:94851080-94851099 | MS.gene064890:CDS | 40.0% | |
| AGACCCAATGTTTCAGCTTA+TGG | + | chr3.3:94850690-94850709 | MS.gene064890:CDS | 40.0% | |
| ATATTTAGTGGCACTTGGTC+GGG | - | chr3.3:94851303-94851322 | None:intergenic | 40.0% | |
| CATATTTAGTGGCACTTGGT+CGG | - | chr3.3:94851304-94851323 | None:intergenic | 40.0% | |
| GCATCATATTTAGTGGCACT+TGG | - | chr3.3:94851308-94851327 | None:intergenic | 40.0% | |
| GTCTCCATAAGCTGAAACAT+TGG | - | chr3.3:94850697-94850716 | None:intergenic | 40.0% | |
| GTCTCCATATGCTGAAACAT+TGG | - | chr3.3:94850775-94850794 | None:intergenic | 40.0% | |
| GTTTCAGCTTATGGAGACAA+TGG | + | chr3.3:94850699-94850718 | MS.gene064890:CDS | 40.0% | |
| TAAGCTGAAACATTGGGTCT+TGG | - | chr3.3:94850690-94850709 | None:intergenic | 40.0% | |
| TATGCTGAAACATTGGGTCT+TGG | - | chr3.3:94851080-94851099 | None:intergenic | 40.0% | |
| TATGCTGAAACATTGGGTGT+TGG | - | chr3.3:94850768-94850787 | None:intergenic | 40.0% | |
| TCCCCATAAGCTGAAATGTT+AGG | - | chr3.3:94851242-94851261 | None:intergenic | 40.0% | |
| ACTCCGAAGCAGTCTTTCAA+AGG | + | chr3.3:94850408-94850427 | MS.gene064890:CDS | 45.0% | |
| TATTTAGTGGCACTTGGTCG+GGG | - | chr3.3:94851302-94851321 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 94850339 | 94851331 | 94850339 | ID=MS.gene064890 |
| chr3.3 | mRNA | 94850339 | 94851331 | 94850339 | ID=MS.gene064890.t1;Parent=MS.gene064890 |
| chr3.3 | exon | 94850339 | 94851331 | 94850339 | ID=MS.gene064890.t1.exon1;Parent=MS.gene064890.t1 |
| chr3.3 | CDS | 94850339 | 94851331 | 94850339 | ID=cds.MS.gene064890.t1;Parent=MS.gene064890.t1 |
| Gene Sequence |
| Protein sequence |
