AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058382


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058382.t1 MTR_8g045520 94.268 157 9 0 1 157 1 157 5.37e-109 307
MS.gene058382.t1 MTR_8g045490 80.892 157 29 1 1 157 1 156 6.84e-92 264
MS.gene058382.t1 MTR_8g045695 80.255 157 30 1 1 157 1 156 2.09e-90 260
MS.gene058382.t1 MTR_8g045400 79.618 157 31 1 1 157 1 156 2.88e-90 259
MS.gene058382.t1 MTR_8g045555 76.159 151 36 0 1 151 1 151 1.73e-86 250
MS.gene058382.t1 MTR_8g045665 77.070 157 35 1 1 157 1 156 9.11e-86 248
MS.gene058382.t1 MTR_8g045300 75.000 156 39 0 1 156 1 156 1.09e-85 248
MS.gene058382.t1 MTR_8g045570 76.159 151 36 0 1 151 1 151 2.18e-85 247
MS.gene058382.t1 MTR_8g045640 75.796 157 37 1 1 157 1 156 2.05e-84 244
MS.gene058382.t1 MTR_8g045735 74.839 155 38 1 1 155 1 154 5.63e-84 244
MS.gene058382.t1 MTR_8g045560 73.248 157 41 1 1 157 1 156 3.39e-81 236
MS.gene058382.t1 MTR_1g030810 42.208 154 86 2 1 152 1 153 3.23e-39 130
MS.gene058382.t1 MTR_1g030820 38.312 154 92 2 1 152 1 153 1.02e-37 126
MS.gene058382.t1 MTR_1g030840 36.538 156 91 4 1 149 1 155 4.35e-28 102
MS.gene058382.t1 MTR_8g012550 32.000 150 99 3 6 154 8 155 6.68e-26 96.7
MS.gene058382.t1 MTR_1g031640 35.374 147 86 5 7 151 7 146 2.54e-24 92.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058382.t1 AT5G28010 34.211 152 97 3 3 152 15 165 1.30e-26 98.6
MS.gene058382.t1 AT5G28010 34.211 152 97 3 3 152 15 165 1.30e-26 98.6
MS.gene058382.t1 AT1G70890 36.184 152 94 3 3 152 7 157 2.51e-26 97.8
MS.gene058382.t1 AT1G23120 32.639 144 92 3 10 153 10 148 3.47e-24 92.0
MS.gene058382.t1 AT1G70840 32.895 152 99 3 3 152 20 170 4.45e-24 92.4
MS.gene058382.t1 AT1G23120 32.639 144 92 3 10 153 60 198 1.51e-23 91.7
MS.gene058382.t1 AT1G70830 32.895 152 99 3 3 152 22 172 3.52e-23 90.1
MS.gene058382.t1 AT1G70830 32.895 152 99 3 3 152 22 172 6.63e-23 90.1
MS.gene058382.t1 AT1G70870 37.086 151 82 4 1 151 1 138 8.12e-23 88.2
MS.gene058382.t1 AT5G28000 31.579 152 101 3 3 152 13 163 9.40e-23 89.0
MS.gene058382.t1 AT1G70830 32.895 152 99 3 3 152 22 172 2.56e-22 89.7
MS.gene058382.t1 AT1G70830 32.895 152 99 3 3 152 137 287 5.23e-22 89.7
MS.gene058382.t1 AT1G70830 28.421 95 67 1 59 152 31 125 3.31e-11 60.1
MS.gene058382.t1 AT1G70830 32.895 152 99 3 3 152 22 172 6.44e-22 90.1
MS.gene058382.t1 AT1G70830 32.895 152 99 3 3 152 184 334 1.57e-21 89.0
MS.gene058382.t1 AT1G70850 32.450 151 99 3 3 151 9 158 7.96e-22 88.2
MS.gene058382.t1 AT1G70850 32.450 151 99 3 3 151 9 158 2.31e-21 88.2
MS.gene058382.t1 AT1G70850 33.099 142 92 3 13 152 175 315 2.96e-21 88.2
MS.gene058382.t1 AT1G70850 32.450 151 99 3 3 151 9 158 2.31e-21 88.2
MS.gene058382.t1 AT1G70850 33.099 142 92 3 13 152 175 315 2.96e-21 88.2
MS.gene058382.t1 AT1G24020 33.117 154 99 3 1 152 1 152 1.98e-19 80.1
MS.gene058382.t1 AT1G24020 33.117 154 99 3 1 152 1 152 1.98e-19 80.1
MS.gene058382.t1 AT4G23670 34.839 155 92 4 1 151 1 150 2.44e-19 79.7
MS.gene058382.t1 AT1G23130 27.152 151 108 2 3 152 10 159 1.49e-18 77.8
MS.gene058382.t1 AT1G70880 28.289 152 106 3 3 152 8 158 2.92e-18 77.0
MS.gene058382.t1 AT1G14930 30.263 152 103 3 1 151 1 150 1.13e-17 75.5
MS.gene058382.t1 AT2G01520 29.605 152 104 3 1 151 1 150 1.51e-16 72.4
MS.gene058382.t1 AT1G35260 28.082 146 101 3 9 152 8 151 4.15e-16 71.2
MS.gene058382.t1 AT4G23680 30.323 155 99 4 1 151 1 150 4.61e-16 71.2
MS.gene058382.t1 AT4G14060 30.323 155 99 4 1 151 1 150 6.21e-15 68.2
MS.gene058382.t1 AT1G14940 29.139 151 104 3 1 150 1 149 4.58e-14 65.9
MS.gene058382.t1 AT1G35310 26.056 142 102 3 13 153 12 151 4.84e-14 65.9
MS.gene058382.t1 AT2G01530 28.289 152 106 3 1 151 1 150 6.94e-14 65.5
MS.gene058382.t1 AT1G14950 28.750 160 105 4 1 156 1 155 1.12e-13 65.1
MS.gene058382.t1 AT1G30990 30.464 151 101 4 1 150 1 148 1.36e-13 64.7
MS.gene058382.t1 AT3G26460 31.013 158 95 6 1 151 1 151 1.35e-11 59.3
MS.gene058382.t1 AT1G14960 26.797 153 109 3 1 152 1 151 7.94e-11 57.4

Find 35 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGGTACTTACAAGCTCTT+TGG 0.306715 3.2:+2644519 MS.gene058382:CDS
ATGGCACTGCTGCTGCTAAA+TGG 0.310932 3.2:+2644604 MS.gene058382:CDS
AAGGTACTTACAAGCTCTTT+GGG 0.349654 3.2:+2644520 MS.gene058382:CDS
CACCCTTATCAATTACTTCA+AGG 0.382574 3.2:-2644581 None:intergenic
AGATATTGATGAGCACTATA+AGG 0.414096 3.2:+2644545 MS.gene058382:CDS
GGTAAACTTATTACTGAACT+TGG 0.415223 3.2:+2643717 MS.gene058382:CDS
CATCCTTGAAGTAATTGATA+AGG 0.438738 3.2:+2644578 MS.gene058382:CDS
ATCTTCACCCTCATGCAGCT+TGG 0.445602 3.2:-2643826 None:intergenic
AAGTAGTCCATCCAGCCATT+TGG 0.456227 3.2:-2644666 None:intergenic
AAACACTGGACTTATGTCAT+AGG 0.470664 3.2:+2643870 MS.gene058382:CDS
ATCCTTGAAGTAATTGATAA+GGG 0.486541 3.2:+2644579 MS.gene058382:CDS
TGAAATAACAACAGATGGTA+AGG 0.489687 3.2:+2644449 MS.gene058382:intron
AGCTGCATGAGGGTGAAGAT+TGG 0.493681 3.2:+2643829 MS.gene058382:CDS
TATGACTTATAGAGCAACCT+TGG 0.515175 3.2:-2644733 None:intergenic
TGATGCTCATCTTGTCAAGC+AGG 0.523708 3.2:+2644710 MS.gene058382:CDS
ATATTGACCCTCCAAATGGC+TGG 0.527845 3.2:+2644655 MS.gene058382:CDS
GAGGATATTGACCCTCCAAA+TGG 0.539495 3.2:+2644651 MS.gene058382:CDS
GTAAACTTATTACTGAACTT+GGG 0.542207 3.2:+2643718 MS.gene058382:CDS
AGATTGGCATCACACTGATT+CGG 0.545548 3.2:+2643845 MS.gene058382:CDS
ATTGATGAACAAAACAAGAA+AGG 0.549109 3.2:+2644501 MS.gene058382:CDS
GAAGTAATTGATAAGGGTGA+TGG 0.549119 3.2:+2644585 MS.gene058382:CDS
TAGTCCATCCAGCCATTTGG+AGG 0.551025 3.2:-2644663 None:intergenic
ACACTGATTCGGTCAAACAC+TGG 0.556043 3.2:+2643856 MS.gene058382:CDS
TGACCCTCCAAATGGCTGGA+TGG 0.557517 3.2:+2644659 MS.gene058382:CDS
GGATCAAAACACCCGCTGAA+AGG 0.578575 3.2:+2643739 MS.gene058382:CDS
TCATCTTGTCAAGCAGGCCA+AGG 0.591246 3.2:+2644716 MS.gene058382:CDS
TCATGAAACCAAGCTGCATG+AGG 0.602712 3.2:+2643818 MS.gene058382:CDS
AGGTACTTACAAGCTCTTTG+GGG 0.621914 3.2:+2644521 MS.gene058382:CDS
CATGAAACCAAGCTGCATGA+GGG 0.630684 3.2:+2643819 MS.gene058382:CDS
AGTTTGAAGAACCTTTCAGC+GGG 0.631322 3.2:-2643750 None:intergenic
GGTACTTACAAGCTCTTTGG+GGG 0.640678 3.2:+2644522 MS.gene058382:CDS
GGTACACACATGTCATGAGA+AGG 0.648648 3.2:+2644470 MS.gene058382:CDS
AGTCCATCCAGCCATTTGGA+GGG 0.649199 3.2:-2644662 None:intergenic
TAGTTTGAAGAACCTTTCAG+CGG 0.662028 3.2:-2643751 None:intergenic
TGAATATGAGAAGATCAATG+AGG 0.699788 3.2:+2644632 MS.gene058382:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAATATTTGTCTAATA+TGG + chr3.2:2644262-2644281 MS.gene058382:intron 10.0%
!! AATCTTACTAAAAAATGTTA+AGG - chr3.2:2644385-2644404 None:intergenic 15.0%
!! TAGACAAATATTATTATTGA+TGG - chr3.2:2644259-2644278 None:intergenic 15.0%
!!! TATTTTATTGAAAATAGCAA+CGG - chr3.2:2644042-2644061 None:intergenic 15.0%
!! AATTAAAGAGATGTACAAAA+AGG + chr3.2:2644354-2644373 MS.gene058382:intron 20.0%
!! ACAAATATTATTATTGATGG+TGG - chr3.2:2644256-2644275 None:intergenic 20.0%
!!! AAACTGCTGTTATTTTTTTT+TGG + chr3.2:2644413-2644432 MS.gene058382:intron 20.0%
!!! GATCTCTAAAAAATGTTTAA+GGG + chr3.2:2644284-2644303 MS.gene058382:intron 20.0%
! AGCAAAAAATTGTACAATAC+TGG - chr3.2:2644220-2644239 None:intergenic 25.0%
! ATCCTTGAAGTAATTGATAA+GGG + chr3.2:2644579-2644598 MS.gene058382:CDS 25.0%
! ATTGATGAACAAAACAAGAA+AGG + chr3.2:2644501-2644520 MS.gene058382:CDS 25.0%
! GATCCTTATTAAAAATGGAA+AGG - chr3.2:2644198-2644217 None:intergenic 25.0%
! GTAAACTTATTACTGAACTT+GGG + chr3.2:2643718-2643737 MS.gene058382:CDS 25.0%
! TACTGGATCCTTATTAAAAA+TGG - chr3.2:2644203-2644222 None:intergenic 25.0%
! TGTTGTACTACAAATGATAA+CGG - chr3.2:2644314-2644333 None:intergenic 25.0%
!! GGATCTCTAAAAAATGTTTA+AGG + chr3.2:2644283-2644302 MS.gene058382:intron 25.0%
!!! GATCCTTTCCATTTTTAATA+AGG + chr3.2:2644192-2644211 MS.gene058382:intron 25.0%
AGATATTGATGAGCACTATA+AGG + chr3.2:2644545-2644564 MS.gene058382:CDS 30.0%
CATCCTTGAAGTAATTGATA+AGG + chr3.2:2644578-2644597 MS.gene058382:CDS 30.0%
GGTAAACTTATTACTGAACT+TGG + chr3.2:2643717-2643736 MS.gene058382:CDS 30.0%
TGAAATAACAACAGATGGTA+AGG + chr3.2:2644449-2644468 MS.gene058382:intron 30.0%
TGAATATGAGAAGATCAATG+AGG + chr3.2:2644632-2644651 MS.gene058382:CDS 30.0%
!! ACCATCTGTTGTTATTTCAT+GGG - chr3.2:2644448-2644467 None:intergenic 30.0%
!! TACCATCTGTTGTTATTTCA+TGG - chr3.2:2644449-2644468 None:intergenic 30.0%
AAACACTGGACTTATGTCAT+AGG + chr3.2:2643870-2643889 MS.gene058382:CDS 35.0%
CAAAATCATCTTGTTGGACT+TGG + chr3.2:2643925-2643944 MS.gene058382:intron 35.0%
CACCCTTATCAATTACTTCA+AGG - chr3.2:2644584-2644603 None:intergenic 35.0%
GAAGTAATTGATAAGGGTGA+TGG + chr3.2:2644585-2644604 MS.gene058382:CDS 35.0%
TAGTTTGAAGAACCTTTCAG+CGG - chr3.2:2643754-2643773 None:intergenic 35.0%
TGCCAACAAAATCATCTTGT+TGG + chr3.2:2643919-2643938 MS.gene058382:intron 35.0%
! AAGGTACTTACAAGCTCTTT+GGG + chr3.2:2644520-2644539 MS.gene058382:CDS 35.0%
! GTCCAACAAGATGATTTTGT+TGG - chr3.2:2643924-2643943 None:intergenic 35.0%
!! AAAGGTACTTACAAGCTCTT+TGG + chr3.2:2644519-2644538 MS.gene058382:CDS 35.0%
AGGTACTTACAAGCTCTTTG+GGG + chr3.2:2644521-2644540 MS.gene058382:CDS 40.0%
AGTTTGAAGAACCTTTCAGC+GGG - chr3.2:2643753-2643772 None:intergenic 40.0%
GCCCATGAAATAACAACAGA+TGG + chr3.2:2644444-2644463 MS.gene058382:intron 40.0%
! AGATTGGCATCACACTGATT+CGG + chr3.2:2643845-2643864 MS.gene058382:CDS 40.0%
AAGTAGTCCATCCAGCCATT+TGG - chr3.2:2644669-2644688 None:intergenic 45.0%
ATATTGACCCTCCAAATGGC+TGG + chr3.2:2644655-2644674 MS.gene058382:CDS 45.0%
CATGAAACCAAGCTGCATGA+GGG + chr3.2:2643819-2643838 MS.gene058382:CDS 45.0%
GAGGATATTGACCCTCCAAA+TGG + chr3.2:2644651-2644670 MS.gene058382:CDS 45.0%
GGTACACACATGTCATGAGA+AGG + chr3.2:2644470-2644489 MS.gene058382:CDS 45.0%
GGTACTTACAAGCTCTTTGG+GGG + chr3.2:2644522-2644541 MS.gene058382:CDS 45.0%
TCATGAAACCAAGCTGCATG+AGG + chr3.2:2643818-2643837 MS.gene058382:CDS 45.0%
TGATGCTCATCTTGTCAAGC+AGG + chr3.2:2644710-2644729 MS.gene058382:CDS 45.0%
!! ACACTGATTCGGTCAAACAC+TGG + chr3.2:2643856-2643875 MS.gene058382:CDS 45.0%
AGCTGCATGAGGGTGAAGAT+TGG + chr3.2:2643829-2643848 MS.gene058382:CDS 50.0%
AGTCCATCCAGCCATTTGGA+GGG - chr3.2:2644665-2644684 None:intergenic 50.0%
ATCTTCACCCTCATGCAGCT+TGG - chr3.2:2643829-2643848 None:intergenic 50.0%
ATGGCACTGCTGCTGCTAAA+TGG + chr3.2:2644604-2644623 MS.gene058382:CDS 50.0%
GGATCAAAACACCCGCTGAA+AGG + chr3.2:2643739-2643758 MS.gene058382:CDS 50.0%
TAGTCCATCCAGCCATTTGG+AGG - chr3.2:2644666-2644685 None:intergenic 50.0%
TCATCTTGTCAAGCAGGCCA+AGG + chr3.2:2644716-2644735 MS.gene058382:CDS 50.0%
TGACCCTCCAAATGGCTGGA+TGG + chr3.2:2644659-2644678 MS.gene058382:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 2643705 2644749 2643705 ID=MS.gene058382
chr3.2 mRNA 2643705 2644749 2643705 ID=MS.gene058382.t1;Parent=MS.gene058382
chr3.2 exon 2643705 2643891 2643705 ID=MS.gene058382.t1.exon1;Parent=MS.gene058382.t1
chr3.2 CDS 2643705 2643891 2643705 ID=cds.MS.gene058382.t1;Parent=MS.gene058382.t1
chr3.2 exon 2644463 2644749 2644463 ID=MS.gene058382.t1.exon2;Parent=MS.gene058382.t1
chr3.2 CDS 2644463 2644749 2644463 ID=cds.MS.gene058382.t1;Parent=MS.gene058382.t1
Gene Sequence

>MS.gene058382

ATGGTGCTTGCTGGTAAACTTATTACTGAACTTGGGATCAAAACACCCGCTGAAAGGTTCTTCAAACTATTTGCATCAGAACTTCATGAAGTGCAAAAACACTGTGAAAGAGTTCATGAAACCAAGCTGCATGAGGGTGAAGATTGGCATCACACTGATTCGGTCAAACACTGGACTTATGTCATAGGTAATTATATTTCTAACTAATTTAACTTGCCAACAAAATCATCTTGTTGGACTTGGATTTTTCTATCATTTAAGTTTATTTACGTGTTGATCACATTACTTAGTTTAGTTTATTATGTTTCATCACAAAAATAAAGCTTCAAGATAGCCGTTGCTATTTTCAATAAAATAGTAAGTTTAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAAAAGATATGTAAAAAAGCAGATCCTTTCCATTTTTAATAAGGATCCAGTATTGTACAATTTTTTGCTGTTTATATTAGATCCACCATCAATAATAATATTTGTCTAATATGGATCTCTAAAAAATGTTTAAGGGTGAACCGTTATCATTTGTAGTACAACAATGAATTTAATAATTAAAAAAATTAAAGAGATGTACAAAAAGGTAGATCCTTAACATTTTTTAGTAAGATTGCACTCTAAAACTGCTGTTATTTTTTTTTGGTATTAATTGCCCATGAAATAACAACAGATGGTAAGGTACACACATGTCATGAGAAGGTTGAAGAAATTGATGAACAAAACAAGAAAGGTACTTACAAGCTCTTTGGGGGAGATATTGATGAGCACTATAAGGACTTTAAGCTCATCCTTGAAGTAATTGATAAGGGTGATGGCACTGCTGCTGCTAAATGGACAGTTGAATATGAGAAGATCAATGAGGATATTGACCCTCCAAATGGCTGGATGGACTACTTATGCAAATGTACTCGAGATATTGATGCTCATCTTGTCAAGCAGGCCAAGGTTGCTCTATAA

Protein sequence

>MS.gene058382.t1

MVLAGKLITELGIKTPAERFFKLFASELHEVQKHCERVHETKLHEGEDWHHTDSVKHWTYVIDGKVHTCHEKVEEIDEQNKKGTYKLFGGDIDEHYKDFKLILEVIDKGDGTAAAKWTVEYEKINEDIDPPNGWMDYLCKCTRDIDAHLVKQAKVAL