AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene04921


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene04921.t1 MTR_3g067510 85.211 142 13 2 1 141 1 135 1.29e-84 244
MS.gene04921.t1 MTR_3g067510 81.690 142 13 3 1 141 1 130 9.47e-79 229
MS.gene04921.t1 MTR_3g067580 78.014 141 26 1 1 141 9 144 7.98e-78 227
MS.gene04921.t1 MTR_3g067430 52.381 147 57 4 1 141 1 140 1.10e-44 143
MS.gene04921.t1 MTR_3g067270 53.623 138 55 3 1 137 1 130 3.57e-42 136
MS.gene04921.t1 MTR_3g467750 53.623 138 55 3 1 137 1 130 3.57e-42 136
MS.gene04921.t1 MTR_3g067280 52.899 138 56 3 1 137 1 130 6.58e-42 136
MS.gene04921.t1 MTR_3g067540 50.000 148 59 4 1 141 1 140 1.23e-41 135
MS.gene04921.t1 MTR_3g067555 50.000 148 59 4 1 141 1 140 1.23e-41 135
MS.gene04921.t1 MTR_3g067437 54.815 135 54 3 1 134 1 129 1.61e-41 134
MS.gene04921.t1 MTR_3g467760 47.445 137 41 2 1 137 1 106 6.44e-37 122
MS.gene04921.t1 MTR_3g067555 50.794 126 52 3 23 141 5 127 1.24e-36 122
MS.gene04921.t1 MTR_3g067500 53.043 115 53 1 27 141 1 114 3.92e-36 120
MS.gene04921.t1 MTR_3g067535 47.945 146 61 7 1 141 1 136 6.73e-36 120
MS.gene04921.t1 MTR_3g067550 50.820 122 50 3 27 141 1 119 1.37e-34 117
MS.gene04921.t1 MTR_3g067555 50.820 122 50 3 27 141 1 119 1.37e-34 117
MS.gene04921.t1 MTR_3g067445 50.000 124 49 4 1 118 1 117 4.73e-33 113
MS.gene04921.t1 MTR_3g463570 43.662 142 71 4 1 140 1 135 5.59e-31 108
MS.gene04921.t1 MTR_6g036620 47.183 142 66 4 1 141 1 134 1.38e-30 107
MS.gene04921.t1 MTR_3g067570 60.000 95 35 2 27 121 1 92 1.46e-30 105
MS.gene04921.t1 MTR_7g056803 41.259 143 77 3 1 141 1 138 4.34e-29 103
MS.gene04921.t1 MTR_3g463570 41.322 121 68 2 22 141 38 156 7.35e-26 95.9
MS.gene04921.t1 MTR_6g017150 44.681 141 66 8 1 137 1 133 7.55e-26 95.5
MS.gene04921.t1 MTR_6g047880 39.286 140 75 4 1 137 1 133 4.19e-25 93.6
MS.gene04921.t1 MTR_3g436100 37.580 157 73 6 1 139 1 150 6.14e-25 93.6
MS.gene04921.t1 MTR_6g017170 42.400 125 61 4 1 122 1 117 1.94e-24 91.3
MS.gene04921.t1 MTR_8g022430 41.135 141 71 6 1 137 1 133 2.02e-24 91.7
MS.gene04921.t1 MTR_6g047900 40.288 139 72 5 1 137 1 130 2.52e-24 91.3
MS.gene04921.t1 MTR_7g056817 37.931 145 80 4 1 141 1 139 7.87e-24 90.1
MS.gene04921.t1 MTR_7g044980 38.411 151 71 4 1 139 1 141 3.82e-22 85.9
MS.gene04921.t1 MTR_3g067830 45.946 111 46 5 1 105 1 103 9.08e-22 84.0
MS.gene04921.t1 MTR_7g044920 39.103 156 70 6 1 139 1 148 1.52e-21 84.7
MS.gene04921.t1 MTR_1g019650 40.625 128 57 5 1 127 1 110 2.07e-21 83.2
MS.gene04921.t1 MTR_8g022400 40.000 140 73 6 1 137 1 132 2.37e-21 84.0
MS.gene04921.t1 MTR_8g022420 41.727 139 66 8 1 134 1 129 2.49e-21 84.0
MS.gene04921.t1 MTR_8g461020 39.640 111 61 3 14 122 10 116 2.88e-21 83.6
MS.gene04921.t1 MTR_4g026590 39.394 132 58 5 20 137 16 139 1.82e-19 79.3
MS.gene04921.t1 MTR_7g029540 36.434 129 72 5 1 126 1 122 3.54e-16 70.1
MS.gene04921.t1 MTR_8g056800 42.667 75 42 1 62 136 9 82 6.34e-14 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAATTCTGATATCAAATA+TGG 0.235651 3.4:+67752800 MS.gene04921:CDS
CCATATTTGATATCAGAATT+AGG 0.279617 3.4:-67752800 None:intergenic
GTCTTCTTGCTTGCTGTATT+CGG 0.286162 3.4:+67752254 MS.gene04921:CDS
ACGGATGCTTTGACTCTTCC+TGG 0.296264 3.4:+67752665 MS.gene04921:CDS
ATCAATCAATGGCTAATGTT+AGG 0.420426 3.4:+67752216 None:intergenic
ACTTACTTCTATTGAAAAGA+AGG 0.442919 3.4:+67752700 MS.gene04921:CDS
GCATGAGACCAACATAAGTT+AGG 0.446047 3.4:-67752734 None:intergenic
TTCTCGATGAAGAAAATAGA+AGG 0.459780 3.4:+67752569 MS.gene04921:CDS
ATGCTTTGACTCTTCCTGGC+TGG 0.478086 3.4:+67752669 MS.gene04921:CDS
GTAAGTGTGGCGCACCAGCC+AGG 0.501795 3.4:-67752683 None:intergenic
GTCTTCTGCTTCAGAGATAG+AGG 0.504252 3.4:-67752834 None:intergenic
TCATGCTGAATGCATAAGAA+AGG 0.509862 3.4:+67752751 MS.gene04921:CDS
AAAATAGAAGGAGCAAAGTG+TGG 0.520085 3.4:+67752581 MS.gene04921:CDS
AAATAGAAGGAGCAAAGTGT+GGG 0.528749 3.4:+67752582 MS.gene04921:CDS
TTCTATTGAAAAGAAGGTTG+AGG 0.541325 3.4:+67752706 MS.gene04921:CDS
TGGTTGTCGTTGCTTTGTCA+CGG 0.545803 3.4:+67752646 MS.gene04921:CDS
AGGAACTACCTAACTTATGT+TGG 0.546452 3.4:+67752726 MS.gene04921:CDS
CATGCTGAATGCATAAGAAA+GGG 0.550324 3.4:+67752752 MS.gene04921:CDS
AATGCATAAGAAAGGGTAGT+GGG 0.610428 3.4:+67752759 MS.gene04921:CDS
AGCAGAAGACTCATTCAAGA+TGG 0.618191 3.4:+67752847 MS.gene04921:CDS
AATAGAAGGAGCAAAGTGTG+GGG 0.621188 3.4:+67752583 MS.gene04921:CDS
GAATGCATAAGAAAGGGTAG+TGG 0.639235 3.4:+67752758 MS.gene04921:CDS
AGAAGGAGCAAAGTGTGGGG+AGG 0.684675 3.4:+67752586 MS.gene04921:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTTTTTTTTTTAATTAG+CGG - chr3.4:67752401-67752420 None:intergenic 10.0%
!! AATGCAAGAATGATATATAT+TGG - chr3.4:67752304-67752323 None:intergenic 20.0%
!!! TTTTTTTTTTTAATTAGCGG+AGG - chr3.4:67752398-67752417 None:intergenic 20.0%
! ACTTACTTCTATTGAAAAGA+AGG + chr3.4:67752700-67752719 MS.gene04921:CDS 25.0%
! CCATATTTGATATCAGAATT+AGG - chr3.4:67752803-67752822 None:intergenic 25.0%
! CCTAATTCTGATATCAAATA+TGG + chr3.4:67752800-67752819 MS.gene04921:CDS 25.0%
ACCCATTATCATATCATATG+TGG + chr3.4:67752351-67752370 MS.gene04921:intron 30.0%
GCCACATATGATATGATAAT+GGG - chr3.4:67752355-67752374 None:intergenic 30.0%
TGCCACATATGATATGATAA+TGG - chr3.4:67752356-67752375 None:intergenic 30.0%
TTACCCACAAAATTTAGCAA+CGG + chr3.4:67752424-67752443 MS.gene04921:intron 30.0%
TTCTATTGAAAAGAAGGTTG+AGG + chr3.4:67752706-67752725 MS.gene04921:CDS 30.0%
TTCTCGATGAAGAAAATAGA+AGG + chr3.4:67752569-67752588 MS.gene04921:CDS 30.0%
! ATTTCCGTTGCTAAATTTTG+TGG - chr3.4:67752431-67752450 None:intergenic 30.0%
! CTTTTCAATAGAAGTAAGTG+TGG - chr3.4:67752699-67752718 None:intergenic 30.0%
! TTTCCGTTGCTAAATTTTGT+GGG - chr3.4:67752430-67752449 None:intergenic 30.0%
AAAATAGAAGGAGCAAAGTG+TGG + chr3.4:67752581-67752600 MS.gene04921:CDS 35.0%
AAATAGAAGGAGCAAAGTGT+GGG + chr3.4:67752582-67752601 MS.gene04921:CDS 35.0%
AATGCATAAGAAAGGGTAGT+GGG + chr3.4:67752759-67752778 MS.gene04921:CDS 35.0%
AGGAACTACCTAACTTATGT+TGG + chr3.4:67752726-67752745 MS.gene04921:CDS 35.0%
CATGCTGAATGCATAAGAAA+GGG + chr3.4:67752752-67752771 MS.gene04921:CDS 35.0%
TCATGCTGAATGCATAAGAA+AGG + chr3.4:67752751-67752770 MS.gene04921:CDS 35.0%
!! CAATTTAATTTTTGCGACGC+TGG + chr3.4:67752620-67752639 MS.gene04921:CDS 35.0%
AATAGAAGGAGCAAAGTGTG+GGG + chr3.4:67752583-67752602 MS.gene04921:CDS 40.0%
AGCAGAAGACTCATTCAAGA+TGG + chr3.4:67752847-67752866 MS.gene04921:CDS 40.0%
GAATGCATAAGAAAGGGTAG+TGG + chr3.4:67752758-67752777 MS.gene04921:CDS 40.0%
GCATGAGACCAACATAAGTT+AGG - chr3.4:67752737-67752756 None:intergenic 40.0%
!! GTCTTCTTGCTTGCTGTATT+CGG + chr3.4:67752254-67752273 MS.gene04921:CDS 40.0%
!! TTTTAATTAGCGGAGGACAG+AGG - chr3.4:67752391-67752410 None:intergenic 40.0%
GTCTTCTGCTTCAGAGATAG+AGG - chr3.4:67752837-67752856 None:intergenic 45.0%
! AATTTTTGCGACGCTGGTGA+TGG + chr3.4:67752626-67752645 MS.gene04921:CDS 45.0%
! TGGTTGTCGTTGCTTTGTCA+CGG + chr3.4:67752646-67752665 MS.gene04921:CDS 45.0%
!! TTATTCTTATAATATAATAA+TGG + chr3.4:67752497-67752516 MS.gene04921:intron 5.0%
ACGGATGCTTTGACTCTTCC+TGG + chr3.4:67752665-67752684 MS.gene04921:CDS 50.0%
ATGCTTTGACTCTTCCTGGC+TGG + chr3.4:67752669-67752688 MS.gene04921:CDS 50.0%
AGAAGGAGCAAAGTGTGGGG+AGG + chr3.4:67752586-67752605 MS.gene04921:CDS 55.0%
TAGCGGAGGACAGAGGCAAG+CGG - chr3.4:67752384-67752403 None:intergenic 60.0%
GTAAGTGTGGCGCACCAGCC+AGG - chr3.4:67752686-67752705 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 67752224 67752922 67752224 ID=MS.gene04921
chr3.4 mRNA 67752224 67752922 67752224 ID=MS.gene04921.t1;Parent=MS.gene04921
chr3.4 exon 67752224 67752275 67752224 ID=MS.gene04921.t1.exon1;Parent=MS.gene04921.t1
chr3.4 CDS 67752224 67752275 67752224 ID=cds.MS.gene04921.t1;Parent=MS.gene04921.t1
chr3.4 exon 67752549 67752922 67752549 ID=MS.gene04921.t1.exon2;Parent=MS.gene04921.t1
chr3.4 CDS 67752549 67752922 67752549 ID=cds.MS.gene04921.t1;Parent=MS.gene04921.t1
Gene Sequence

>MS.gene04921

ATGGCTAATGTTAGGCTTGCTTCTTTTGCTGTCTTCTTGCTTGCTGTATTCGGTATATATTTCAATTATCTTTCTCACCAATATATATCATTCTTGCATTTCTATTTATGTTTAATAGAAATTAACTACCCATTATCATATCATATGTGGCATGTATCCGCTTGCCTCTGTCCTCCGCTAATTAAAAAAAAAAAACTAAATTACCCACAAAATTTAGCAACGGAAATAATAAATTTCAGTTTTTTTTTTTCTATCTAAAGTTAGTAAATAATTTTATTCTTATAATATAATAATGGTATGTGTATGTTGTTCAAACTTTAATTAGCTTTGTCATTTACAGTAATGTTCTCGATGAAGAAAATAGAAGGAGCAAAGTGTGGGGAGGCTTGTGACACGCAATTTAATTTTTGCGACGCTGGTGATGGTTGTCGTTGCTTTGTCACGGATGCTTTGACTCTTCCTGGCTGGTGCGCCACACTTACTTCTATTGAAAAGAAGGTTGAGGAACTACCTAACTTATGTTGGTCTCATGCTGAATGCATAAGAAAGGGTAGTGGGAATTATTGTGCTCATCTTCCTAATTCTGATATCAAATATGGCTTCTGTTTTGCCTCTATCTCTGAAGCAGAAGACTCATTCAAGATGGCAGCTAGCACTATATACAAAAGAGACTTCTTGAAGATGTCTGTTTCTGCTTAA

Protein sequence

>MS.gene04921.t1

MANVRLASFAVFLLAVFALSFTVMFSMKKIEGAKCGEACDTQFNFCDAGDGCRCFVTDALTLPGWCATLTSIEKKVEELPNLCWSHAECIRKGSGNYCAHLPNSDIKYGFCFASISEAEDSFKMAASTIYKRDFLKMSVSA