AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene064902

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064902.t1 MTR_1g045550 98.807 503 6 0 1 503 1 503 0.0 1013
MS.gene064902.t1 MTR_7g098930 74.948 483 119 2 1 482 1 482 0.0 736
MS.gene064902.t1 MTR_1g079760 74.359 507 118 5 1 498 1 504 0.0 734
MS.gene064902.t1 MTR_7g113340 73.444 482 126 2 1 481 1 481 0.0 719
MS.gene064902.t1 MTR_3g463730 66.372 452 147 4 45 493 11 460 0.0 594
MS.gene064902.t1 MTR_7g115050 27.007 411 239 13 113 501 100 471 1.92e-23 103
MS.gene064902.t1 MTR_1g036410 27.097 465 272 16 48 482 24 451 1.26e-22 101
MS.gene064902.t1 MTR_8g074750 25.979 485 295 15 48 502 30 480 4.84e-20 93.2
MS.gene064902.t1 MTR_7g069640 26.638 473 280 16 48 485 22 462 1.38e-18 89.0
MS.gene064902.t1 MTR_8g095040 23.958 480 305 11 48 496 29 479 1.05e-16 83.2
MS.gene064902.t1 MTR_4g017820 26.971 482 281 16 48 495 22 466 3.50e-15 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064902.t1 AT4G13510 80.478 502 88 3 5 499 2 500 0.0 828
MS.gene064902.t1 AT3G24300 77.465 497 99 4 6 497 7 495 0.0 779
MS.gene064902.t1 AT3G24290 79.237 472 95 1 6 477 7 475 0.0 769
MS.gene064902.t1 AT1G64780 76.190 483 104 7 27 500 31 511 0.0 738
MS.gene064902.t1 AT4G28700 74.745 491 116 4 5 489 6 494 0.0 712
MS.gene064902.t1 AT2G38290 27.233 459 283 13 48 485 20 448 5.45e-23 102
MS.gene064902.t1 AT2G38290 27.809 356 221 10 136 485 7 332 1.41e-18 87.8

Find 152 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGACCCTATGGGTTATTTA+TGG 0.176472 1.4:-29828155 MS.gene064902:CDS
GGCACATGTGATACAAATTT+TGG 0.187812 1.4:-29828110 MS.gene064902:CDS
TGGTGGTATTGCTGGTTTAT+GGG 0.202357 1.4:-29828876 MS.gene064902:CDS
AGTGCCTCTCTTGTTGTTCT+TGG 0.219537 1.4:-29828782 MS.gene064902:CDS
TTGGTGGTATTGCTGGTTTA+TGG 0.237577 1.4:-29828877 MS.gene064902:CDS
CCAGGTTTATCCGGGTAAAC+TGG 0.249185 1.4:-29828179 MS.gene064902:CDS
TTCATTGGAAAACATTTCTT+TGG 0.268354 1.4:-29829178 MS.gene064902:CDS
ATTCTCAGTCCTTATAATTC+AGG 0.269091 1.4:-29828704 MS.gene064902:intron
CTGTGGTTTCTCATTGGTTT+TGG 0.272044 1.4:-29828994 MS.gene064902:CDS
GCTTCGGCGTTGGTGGCGTT+GGG 0.277122 1.4:+29829466 None:intergenic
TTCTTGGCACTTTCATGCTT+TGG 0.277934 1.4:-29828766 MS.gene064902:CDS
GGTGGTGGAGGGAAATTATT+AGG 0.301790 1.4:-29828133 MS.gene064902:CDS
CATATGGTTGGTGGTATTGC+TGG 0.320936 1.4:-29828884 MS.gene064902:CDS
GGCTTCGGCGTTGGTGGCGT+TGG 0.324200 1.4:+29829465 None:intergenic
ACACCTTCAAATGGTTTCAT+TGG 0.325076 1.4:-29829193 MS.gene064902:CDS
GATGAAATTTGCGGCGGCTT+CGG 0.336367 1.4:+29829450 None:intergenic
CTTATTGAAGGTCCGCGAAT+TGG 0.341909 1.4:-29828848 MS.gene064902:CDS
ATTGGACGGTTCGATCATTC+TGG 0.345994 1.4:-29828830 MS.gene064902:CDS
TTTACCCTGTGGTTTCTCAT+TGG 0.351616 1.4:-29829000 MS.gene064902:CDS
CGACCCTATGGGTTATTTAT+GGG 0.352551 1.4:-29828154 MS.gene064902:CDS
GGCACTTTCATGCTTTGGTT+TGG 0.354842 1.4:-29828761 MS.gene064902:CDS
TGTTTGACACTGCTGTGAAC+TGG 0.360310 1.4:+29829422 None:intergenic
TGTAATTGTGCTGCTTCTAA+TGG 0.364233 1.4:+29828265 None:intergenic
GATGAAGGATCACATAGAAA+TGG 0.373243 1.4:-29827947 MS.gene064902:CDS
CTTTCATGCTTTGGTTTGGT+TGG 0.380571 1.4:-29828757 MS.gene064902:CDS
TGGGTGGGTAAGTGCAACTA+TGG 0.381238 1.4:-29828080 MS.gene064902:CDS
TGGCTCCGGTGTTGTTCATA+TGG 0.393312 1.4:-29828900 MS.gene064902:CDS
GTATGTGAACCAGGTTTATC+CGG 0.405498 1.4:-29828188 MS.gene064902:CDS
GGTAAACTGGGTCGACCCTA+TGG 0.406965 1.4:-29828166 MS.gene064902:CDS
CGTTAGAGCTGCCGTGCAAC+CGG 0.414595 1.4:+29828483 None:intergenic
TCGCAGCTCGCGCAGTTCAT+CGG 0.418885 1.4:-29829490 MS.gene064902:CDS
TGAAGATGAACTTGCGGGTA+TGG 0.421273 1.4:-29828008 MS.gene064902:CDS
AGTTAACTACCTGAATTATA+AGG 0.425481 1.4:+29828695 None:intergenic
TCAACTGAAGATGAACTTGC+GGG 0.428559 1.4:-29828013 MS.gene064902:CDS
AAAGAATTCTCTCCGGTCAC+TGG 0.431120 1.4:-29828447 MS.gene064902:CDS
ATGCTTTGGTTTGGTTGGTA+TGG 0.433222 1.4:-29828752 MS.gene064902:CDS
TACAATCACAAATCTGTAGT+TGG 0.437561 1.4:+29827878 None:intergenic
AGAATCTTGGTGAAGGAACC+AGG 0.441335 1.4:+29828722 None:intergenic
GTAAGCTGAGAATAGAAGAT+AGG 0.441751 1.4:+29829366 None:intergenic
ATTTGCGGCGGCTTCGGCGT+TGG 0.442397 1.4:+29829456 None:intergenic
TACACGGTGGGTGTGGCGCG+TGG 0.444248 1.4:-29828246 MS.gene064902:CDS
GGTTGGTATGGTTTCAACCC+TGG 0.447548 1.4:-29828740 MS.gene064902:CDS
GGTGTTCCGTTGTAGAACCT+TGG 0.448411 1.4:-29828369 MS.gene064902:CDS
AAAACATTTCTTTGGTCTGA+AGG 0.450771 1.4:-29829170 MS.gene064902:CDS
GGGTTATTTATGGGTGGTGG+AGG 0.451374 1.4:-29828145 MS.gene064902:CDS
TTTCCAATGAAACCATTTGA+AGG 0.457318 1.4:+29829190 None:intergenic
TGCGGCGGCTTCGGCGTTGG+TGG 0.461817 1.4:+29829459 None:intergenic
GTGTTCCGTTGTAGAACCTT+GGG 0.462761 1.4:-29828368 MS.gene064902:CDS
TATGGGTTATTTATGGGTGG+TGG 0.467977 1.4:-29828148 MS.gene064902:CDS
TTATTCAAAACAAAGAATAG+TGG 0.471377 1.4:+29828055 None:intergenic
ATGAAGGATCACATAGAAAT+GGG 0.473794 1.4:-29827946 MS.gene064902:CDS
AACGTCCTCGACGCTGCTGC+TGG 0.476966 1.4:-29829259 MS.gene064902:CDS
ATAAGCAAACCCACCATGTC+GGG 0.481927 1.4:+29827979 None:intergenic
ACACGGTGGGTGTGGCGCGT+GGG 0.483461 1.4:-29828245 MS.gene064902:CDS
ATGGAGTGCGGTGGGGAGGA+CGG 0.485440 1.4:-29828524 MS.gene064902:CDS
GGTGGTATTGCTGGTTTATG+GGG 0.486687 1.4:-29828875 MS.gene064902:CDS
ATTTACTCATCGTTTCTCAC+AGG 0.487248 1.4:-29829028 MS.gene064902:CDS
TCCGGTGTTGTTCATATGGT+TGG 0.488732 1.4:-29828896 MS.gene064902:CDS
CACGGTGGGTGTGGCGCGTG+GGG 0.489854 1.4:-29828244 MS.gene064902:CDS
GCTTATGCTTATGAAGATGA+AGG 0.489963 1.4:-29827962 MS.gene064902:CDS
GTTTGACACTGCTGTGAACT+GGG 0.490561 1.4:+29829423 None:intergenic
GTTGGTAAGCATGATATTCA+TGG 0.495119 1.4:+29829279 None:intergenic
GGTTTCGCTGCGATAACCGC+CGG 0.495174 1.4:-29828391 MS.gene064902:CDS
ATTATAAGGACTGAGAATCT+TGG 0.495971 1.4:+29828709 None:intergenic
TGAATAAATTGAAGTTGTTG+AGG 0.497576 1.4:-29828039 MS.gene064902:CDS
TAGGTACTTATTATGGGCAA+TGG 0.500219 1.4:-29828543 MS.gene064902:CDS
GGCAATGGAGTGCGGTGGGG+AGG 0.501855 1.4:-29828528 MS.gene064902:CDS
GTGGTGGAGGGAAATTATTA+GGG 0.501970 1.4:-29828132 MS.gene064902:CDS
TTATGGGGAGCACTTATTGA+AGG 0.501971 1.4:-29828860 MS.gene064902:CDS
TATGGGCAATGGAGTGCGGT+GGG 0.503029 1.4:-29828532 MS.gene064902:CDS
AAACAAAAGGTCCCCGGTGT+TGG 0.506596 1.4:+29828946 None:intergenic
TGACTCCGGTGCCAAACAAA+AGG 0.510137 1.4:+29828933 None:intergenic
TGGGCTAGCGCAACCAACAC+CGG 0.511581 1.4:-29828959 MS.gene064902:CDS
ATCGGTCACGTTCCAGTGAC+CGG 0.514328 1.4:+29828435 None:intergenic
AGCTGCGAAACAGAGCATTC+CGG 0.515010 1.4:+29829505 None:intergenic
GGTCGAGCCGTGGCACTTCG+CGG 0.515352 1.4:-29828809 MS.gene064902:CDS
CCAGTTTACCCGGATAAACC+TGG 0.516204 1.4:+29828179 None:intergenic
CAGGTTTATCCGGGTAAACT+GGG 0.517085 1.4:-29828178 MS.gene064902:CDS
TTCGGGAAAAGAATTCTCTC+CGG 0.517363 1.4:-29828454 MS.gene064902:CDS
TTGAAGGTCCGCGAATTGGA+CGG 0.518069 1.4:-29828844 MS.gene064902:CDS
GATGTCTGCAACGGATTACT+CGG 0.521512 1.4:-29828415 MS.gene064902:CDS
GTGCAACAAACTCGCGGAGA+AGG 0.523705 1.4:-29828302 MS.gene064902:CDS
TGCGCTAGCCCAACCATCAC+CGG 0.524846 1.4:+29828970 None:intergenic
GGTTATTTATGGGTGGTGGA+GGG 0.528393 1.4:-29828144 MS.gene064902:CDS
GGGCTAGCGCAACCAACACC+GGG 0.530553 1.4:-29828958 MS.gene064902:CDS
CCCACCAAACATGTCGCTGC+CGG 0.531903 1.4:-29829524 None:intergenic
GTTTCGCTGCGATAACCGCC+GGG 0.534726 1.4:-29828390 MS.gene064902:CDS
GCGGTGACGACGACATTAGC+CGG 0.538079 1.4:-29828502 MS.gene064902:CDS
TGATCGAACCGTCCAATTCG+CGG 0.541228 1.4:+29828836 None:intergenic
CCCTATGGGTTATTTATGGG+TGG 0.544513 1.4:-29828151 MS.gene064902:CDS
GCACAATTACACGGTGGGTG+TGG 0.544851 1.4:-29828253 MS.gene064902:CDS
CCTTGGGCAGCAATTGTATG+TGG 0.545119 1.4:-29828352 MS.gene064902:CDS
TTTACTCATCGTTTCTCACA+GGG 0.546630 1.4:-29829027 MS.gene064902:CDS
ATCTGTAGTTGGAGTAGGAG+TGG 0.549034 1.4:+29827889 None:intergenic
TCGTTCCGCAATAGAGCCGC+TGG 0.550227 1.4:+29829072 None:intergenic
ATAGAAATGGGACTCAATTG+AGG 0.554439 1.4:-29827934 MS.gene064902:CDS
AGGTTCTACAACGGAACACC+CGG 0.560703 1.4:+29828372 None:intergenic
GACGACATTAGCCGGTTGCA+CGG 0.562542 1.4:-29828494 MS.gene064902:CDS
ACTGTGTCCGCGAAGTGCCA+CGG 0.564948 1.4:+29828802 None:intergenic
GTCCTCGACGCTGCTGCTGG+TGG 0.568629 1.4:-29829256 MS.gene064902:CDS
CAAGTTGCATAGAGAAAACA+AGG 0.569214 1.4:+29829344 None:intergenic
AAAAGAAGAGTATGTGAACC+AGG 0.570298 1.4:-29828197 MS.gene064902:CDS
CTTCGGCGTTGGTGGCGTTG+GGG 0.570819 1.4:+29829467 None:intergenic
AACTGAACCAGCACAAAGCA+TGG 0.571080 1.4:+29829315 None:intergenic
CACAGGGTTTGTTTACCCTG+TGG 0.571684 1.4:-29829011 MS.gene064902:CDS
CACAAATCTGTAGTTGGAGT+AGG 0.574103 1.4:+29827884 None:intergenic
TATGTGAACCAGGTTTATCC+GGG 0.576473 1.4:-29828187 MS.gene064902:CDS
TTCAACTGAAGATGAACTTG+CGG 0.585243 1.4:-29828014 MS.gene064902:CDS
TGAAGGTGTTCCAAAAGCAA+AGG 0.585376 1.4:+29829207 None:intergenic
GGTGTTGTTCATATGGTTGG+TGG 0.587357 1.4:-29828893 MS.gene064902:CDS
GTAAACTGGGTCGACCCTAT+GGG 0.589828 1.4:-29828165 MS.gene064902:CDS
TAGGGTCGACCCAGTTTACC+CGG 0.590994 1.4:+29828169 None:intergenic
TATCACCAGCGGCTCTATTG+CGG 0.592562 1.4:-29829077 MS.gene064902:CDS
GCCAGCAAAATCAATGACTC+CGG 0.596809 1.4:+29828919 None:intergenic
GGGTGGGTAAGTGCAACTAT+GGG 0.598468 1.4:-29828079 MS.gene064902:CDS
TGGATTTGACCCGACATGGT+GGG 0.598703 1.4:-29827988 MS.gene064902:CDS
GGTGCCAAACAAAAGGTCCC+CGG 0.599775 1.4:+29828940 None:intergenic
CATAAGCAAACCCACCATGT+CGG 0.601038 1.4:+29827978 None:intergenic
CAAAACCAATGAGAAACCAC+AGG 0.603230 1.4:+29828995 None:intergenic
ACCACCCATAAATAACCCAT+AGG 0.609604 1.4:+29828150 None:intergenic
TTATGGGCAATGGAGTGCGG+TGG 0.609738 1.4:-29828533 MS.gene064902:CDS
CTGGGTGCAGATGAAATTTG+CGG 0.612969 1.4:+29829441 None:intergenic
GAGTGCGGTGGGGAGGACGG+CGG 0.614067 1.4:-29828521 MS.gene064902:CDS
CGATCATTCTGGTCGAGCCG+TGG 0.616254 1.4:-29828819 MS.gene064902:CDS
CGCCACCAGCAGCAGCGTCG+AGG 0.620566 1.4:+29829254 None:intergenic
AGGACTGAGAATCTTGGTGA+AGG 0.622311 1.4:+29828715 None:intergenic
CCACCCATAAATAACCCATA+GGG 0.625187 1.4:+29828151 None:intergenic
GGTATGGATTTGACCCGACA+TGG 0.625832 1.4:-29827992 MS.gene064902:CDS
GAGTAATCCGTTGCAGACAT+CGG 0.629600 1.4:+29828417 None:intergenic
AGCAGCAGCGTCGAGGACGT+TGG 0.631989 1.4:+29829261 None:intergenic
AAGCAGCACAATTACACGGT+GGG 0.632622 1.4:-29828258 MS.gene064902:CDS
AACGTGACCGATGTCTGCAA+CGG 0.633445 1.4:-29828424 MS.gene064902:CDS
ATGGATTTGACCCGACATGG+TGG 0.636723 1.4:-29827989 MS.gene064902:CDS
TGGTGGAGGGAAATTATTAG+GGG 0.638481 1.4:-29828131 MS.gene064902:CDS
CCACATACAATTGCTGCCCA+AGG 0.640235 1.4:+29828352 None:intergenic
ACCAACCATATGAACAACAC+CGG 0.643450 1.4:+29828895 None:intergenic
AATCGCGTGCAACAAACTCG+CGG 0.668565 1.4:-29828308 MS.gene064902:CDS
TTAGAAGCAGCACAATTACA+CGG 0.668867 1.4:-29828262 MS.gene064902:CDS
GGTGCAGATGAAATTTGCGG+CGG 0.669046 1.4:+29829444 None:intergenic
ATGGGCAATGGAGTGCGGTG+GGG 0.682220 1.4:-29828531 MS.gene064902:CDS
TTATTATGGGCAATGGAGTG+CGG 0.683210 1.4:-29828536 MS.gene064902:CDS
GAATCTTGGTGAAGGAACCA+GGG 0.685698 1.4:+29828723 None:intergenic
GTCTGCAACGGATTACTCGG+CGG 0.686227 1.4:-29828412 MS.gene064902:CDS
AGAGCCGCTGGTGATACCAG+CGG 0.694743 1.4:+29829084 None:intergenic
AGTGCCAAGAACAACAAGAG+AGG 0.695048 1.4:+29828778 None:intergenic
TGCTGCCCAAGGTTCTACAA+CGG 0.701646 1.4:+29828363 None:intergenic
GCAGCCGCTGGTATCACCAG+CGG 0.703921 1.4:-29829088 MS.gene064902:CDS
GCACGCGATTAAAACAATAG+CGG 0.740384 1.4:+29828321 None:intergenic
GGCTAGCGCAACCAACACCG+GGG 0.741776 1.4:-29828957 MS.gene064902:CDS
GAAGCAGCACAATTACACGG+TGG 0.750534 1.4:-29828259 MS.gene064902:CDS
TTCTACAACGGAACACCCGG+CGG 0.760823 1.4:+29828375 None:intergenic
AAAACCAATGAGAAACCACA+GGG 0.764570 1.4:+29828996 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAAATTAAAATTTGAAA+AGG + chr1.4:29828816-29828835 None:intergenic 10.0%
!!! TTAAATTTTTATGGAATTTT+AGG - chr1.4:29828835-29828854 MS.gene064902:CDS 10.0%
!!! ATACAAATTTTGGTTATTAT+TGG - chr1.4:29829297-29829316 MS.gene064902:CDS 15.0%
!!! TACAAATTTTGGTTATTATT+GGG - chr1.4:29829298-29829317 MS.gene064902:CDS 15.0%
!! TTATTCAAAACAAAGAATAG+TGG + chr1.4:29829345-29829364 None:intergenic 20.0%
!!! GAATTTTAGGTACTTATTAT+GGG - chr1.4:29828848-29828867 MS.gene064902:CDS 20.0%
! AGTTAACTACCTGAATTATA+AGG + chr1.4:29828705-29828724 None:intergenic 25.0%
! TGAATAAATTGAAGTTGTTG+AGG - chr1.4:29829358-29829377 MS.gene064902:CDS 25.0%
! TTCATTGGAAAACATTTCTT+TGG - chr1.4:29828219-29828238 MS.gene064902:CDS 25.0%
!! ACTAACTACTTTCATTGTTT+TGG - chr1.4:29828739-29828758 MS.gene064902:CDS 25.0%
!! CTGTAATCAAAATTTTCAGT+CGG + chr1.4:29828258-29828277 None:intergenic 25.0%
!!! AAATTTTGGTTATTATTGGG+TGG - chr1.4:29829301-29829320 MS.gene064902:CDS 25.0%
!!! AATTTTGGTTATTATTGGGT+GGG - chr1.4:29829302-29829321 MS.gene064902:CDS 25.0%
!!! ACAGTTATTTTCTCTATCAA+TGG - chr1.4:29828274-29828293 MS.gene064902:CDS 25.0%
!!! CAGTTATTTTCTCTATCAAT+GGG - chr1.4:29828275-29828294 MS.gene064902:CDS 25.0%
!!! GGAATTTTAGGTACTTATTA+TGG - chr1.4:29828847-29828866 MS.gene064902:CDS 25.0%
AAAACATTTCTTTGGTCTGA+AGG - chr1.4:29828227-29828246 MS.gene064902:CDS 30.0%
ATGAAGGATCACATAGAAAT+GGG - chr1.4:29829451-29829470 MS.gene064902:CDS 30.0%
ATTATAAGGACTGAGAATCT+TGG + chr1.4:29828691-29828710 None:intergenic 30.0%
ATTCTCAGTCCTTATAATTC+AGG - chr1.4:29828693-29828712 MS.gene064902:intron 30.0%
TTTCCAATGAAACCATTTGA+AGG + chr1.4:29828210-29828229 None:intergenic 30.0%
AAAACCAATGAGAAACCACA+GGG + chr1.4:29828404-29828423 None:intergenic 35.0%
ACACCTTCAAATGGTTTCAT+TGG - chr1.4:29828204-29828223 MS.gene064902:CDS 35.0%
ATAGAAATGGGACTCAATTG+AGG - chr1.4:29829463-29829482 MS.gene064902:CDS 35.0%
ATTTACTCATCGTTTCTCAC+AGG - chr1.4:29828369-29828388 MS.gene064902:CDS 35.0%
CAAGTTGCATAGAGAAAACA+AGG + chr1.4:29828056-29828075 None:intergenic 35.0%
GATGAAGGATCACATAGAAA+TGG - chr1.4:29829450-29829469 MS.gene064902:CDS 35.0%
GCTTATGCTTATGAAGATGA+AGG - chr1.4:29829435-29829454 MS.gene064902:CDS 35.0%
GTAAGCTGAGAATAGAAGAT+AGG + chr1.4:29828034-29828053 None:intergenic 35.0%
TAGGTACTTATTATGGGCAA+TGG - chr1.4:29828854-29828873 MS.gene064902:CDS 35.0%
TTAGAAGCAGCACAATTACA+CGG - chr1.4:29829135-29829154 MS.gene064902:CDS 35.0%
TTCAACAAGAGCTTTCCATT+AGG + chr1.4:29828770-29828789 None:intergenic 35.0%
TTCAACTGAAGATGAACTTG+CGG - chr1.4:29829383-29829402 MS.gene064902:CDS 35.0%
TTTACTCATCGTTTCTCACA+GGG - chr1.4:29828370-29828389 MS.gene064902:CDS 35.0%
! AAAAGAAGAGTATGTGAACC+AGG - chr1.4:29829200-29829219 MS.gene064902:CDS 35.0%
! CTCTTCTTTTGCAAACAAAG+CGG + chr1.4:29829190-29829209 None:intergenic 35.0%
! GGCACATGTGATACAAATTT+TGG - chr1.4:29829287-29829306 MS.gene064902:CDS 35.0%
! GTTGGTAAGCATGATATTCA+TGG + chr1.4:29828121-29828140 None:intergenic 35.0%
!! CTTGTTTTCTCTATGCAACT+TGG - chr1.4:29828054-29828073 MS.gene064902:CDS 35.0%
!! TGTAATTGTGCTGCTTCTAA+TGG + chr1.4:29829135-29829154 None:intergenic 35.0%
!!! ATTGTTTTGGATGCACCTAA+TGG - chr1.4:29828752-29828771 MS.gene064902:CDS 35.0%
ACCAACCATATGAACAACAC+CGG + chr1.4:29828505-29828524 None:intergenic 40.0%
ACCACCCATAAATAACCCAT+AGG + chr1.4:29829250-29829269 None:intergenic 40.0%
CAAAACCAATGAGAAACCAC+AGG + chr1.4:29828405-29828424 None:intergenic 40.0%
CACAAATCTGTAGTTGGAGT+AGG + chr1.4:29829516-29829535 None:intergenic 40.0%
CCACCCATAAATAACCCATA+GGG + chr1.4:29829249-29829268 None:intergenic 40.0%
GCACGCGATTAAAACAATAG+CGG + chr1.4:29829079-29829098 None:intergenic 40.0%
GTATGTGAACCAGGTTTATC+CGG - chr1.4:29829209-29829228 MS.gene064902:CDS 40.0%
TATGTGAACCAGGTTTATCC+GGG - chr1.4:29829210-29829229 MS.gene064902:CDS 40.0%
TCAACTGAAGATGAACTTGC+GGG - chr1.4:29829384-29829403 MS.gene064902:CDS 40.0%
TGGTGGAGGGAAATTATTAG+GGG - chr1.4:29829266-29829285 MS.gene064902:CDS 40.0%
TTATTATGGGCAATGGAGTG+CGG - chr1.4:29828861-29828880 MS.gene064902:CDS 40.0%
TTCGGGAAAAGAATTCTCTC+CGG - chr1.4:29828943-29828962 MS.gene064902:CDS 40.0%
TTCTTGGCACTTTCATGCTT+TGG - chr1.4:29828631-29828650 MS.gene064902:intron 40.0%
TTTACCCTGTGGTTTCTCAT+TGG - chr1.4:29828397-29828416 MS.gene064902:CDS 40.0%
! CGACCCTATGGGTTATTTAT+GGG - chr1.4:29829243-29829262 MS.gene064902:CDS 40.0%
! GGCGTTTTCTACTATCTCTT+CGG - chr1.4:29828162-29828181 MS.gene064902:CDS 40.0%
! GTGGTGGAGGGAAATTATTA+GGG - chr1.4:29829265-29829284 MS.gene064902:CDS 40.0%
! TATGGGTTATTTATGGGTGG+TGG - chr1.4:29829249-29829268 MS.gene064902:CDS 40.0%
! TCGACCCTATGGGTTATTTA+TGG - chr1.4:29829242-29829261 MS.gene064902:CDS 40.0%
! TTCGGTTTTGCCTTTGCTTT+TGG - chr1.4:29828180-29828199 MS.gene064902:CDS 40.0%
!! ATGCTTTGGTTTGGTTGGTA+TGG - chr1.4:29828645-29828664 MS.gene064902:intron 40.0%
!! CTTTCATGCTTTGGTTTGGT+TGG - chr1.4:29828640-29828659 MS.gene064902:intron 40.0%
!! TGAAGGTGTTCCAAAAGCAA+AGG + chr1.4:29828193-29828212 None:intergenic 40.0%
!! TGGTGGTATTGCTGGTTTAT+GGG - chr1.4:29828521-29828540 MS.gene064902:CDS 40.0%
!! TTATGGGGAGCACTTATTGA+AGG - chr1.4:29828537-29828556 MS.gene064902:CDS 40.0%
!! TTGGTGGTATTGCTGGTTTA+TGG - chr1.4:29828520-29828539 MS.gene064902:CDS 40.0%
!!! CTGTGGTTTCTCATTGGTTT+TGG - chr1.4:29828403-29828422 MS.gene064902:CDS 40.0%
!!! GCTTTTGGAACACCTTCAAA+TGG - chr1.4:29828195-29828214 MS.gene064902:CDS 40.0%
!!! GTTTCTCATTGGTTTTGGTC+CGG - chr1.4:29828408-29828427 MS.gene064902:CDS 40.0%
AAAGAATTCTCTCCGGTCAC+TGG - chr1.4:29828950-29828969 MS.gene064902:CDS 45.0%
AACTGAACCAGCACAAAGCA+TGG + chr1.4:29828085-29828104 None:intergenic 45.0%
AAGCAGCACAATTACACGGT+GGG - chr1.4:29829139-29829158 MS.gene064902:CDS 45.0%
AGAATCTTGGTGAAGGAACC+AGG + chr1.4:29828678-29828697 None:intergenic 45.0%
AGGACTGAGAATCTTGGTGA+AGG + chr1.4:29828685-29828704 None:intergenic 45.0%
AGTGCCAAGAACAACAAGAG+AGG + chr1.4:29828622-29828641 None:intergenic 45.0%
ATAAGCAAACCCACCATGTC+GGG + chr1.4:29829421-29829440 None:intergenic 45.0%
ATCTGTAGTTGGAGTAGGAG+TGG + chr1.4:29829511-29829530 None:intergenic 45.0%
ATTGGACGGTTCGATCATTC+TGG - chr1.4:29828567-29828586 MS.gene064902:intron 45.0%
CAGGTTTATCCGGGTAAACT+GGG - chr1.4:29829219-29829238 MS.gene064902:CDS 45.0%
CATAAGCAAACCCACCATGT+CGG + chr1.4:29829422-29829441 None:intergenic 45.0%
CTGGGTGCAGATGAAATTTG+CGG + chr1.4:29827959-29827978 None:intergenic 45.0%
CTTATTGAAGGTCCGCGAAT+TGG - chr1.4:29828549-29828568 MS.gene064902:intron 45.0%
GAATCTTGGTGAAGGAACCA+GGG + chr1.4:29828677-29828696 None:intergenic 45.0%
GAGTAATCCGTTGCAGACAT+CGG + chr1.4:29828983-29829002 None:intergenic 45.0%
GCCAGCAAAATCAATGACTC+CGG + chr1.4:29828481-29828500 None:intergenic 45.0%
GGCACTTTCATGCTTTGGTT+TGG - chr1.4:29828636-29828655 MS.gene064902:intron 45.0%
GTGTTCCGTTGTAGAACCTT+GGG - chr1.4:29829029-29829048 MS.gene064902:CDS 45.0%
GTTTGACACTGCTGTGAACT+GGG + chr1.4:29827977-29827996 None:intergenic 45.0%
TGAAGATGAACTTGCGGGTA+TGG - chr1.4:29829389-29829408 MS.gene064902:CDS 45.0%
TGTTTGACACTGCTGTGAAC+TGG + chr1.4:29827978-29827997 None:intergenic 45.0%
! CCCTATGGGTTATTTATGGG+TGG - chr1.4:29829246-29829265 MS.gene064902:CDS 45.0%
!! AGTGCCTCTCTTGTTGTTCT+TGG - chr1.4:29828615-29828634 MS.gene064902:intron 45.0%
!! CAGCTCTAACGACACTTTTC+GGG - chr1.4:29828926-29828945 MS.gene064902:CDS 45.0%
!! CATATGGTTGGTGGTATTGC+TGG - chr1.4:29828513-29828532 MS.gene064902:CDS 45.0%
!! GATGTCTGCAACGGATTACT+CGG - chr1.4:29828982-29829001 MS.gene064902:CDS 45.0%
!! GCAGCTCTAACGACACTTTT+CGG - chr1.4:29828925-29828944 MS.gene064902:CDS 45.0%
!! GGTGGTATTGCTGGTTTATG+GGG - chr1.4:29828522-29828541 MS.gene064902:CDS 45.0%
!! GGTGGTGGAGGGAAATTATT+AGG - chr1.4:29829264-29829283 MS.gene064902:CDS 45.0%
!! GGTGTTGTTCATATGGTTGG+TGG - chr1.4:29828504-29828523 MS.gene064902:CDS 45.0%
!! GGTTATTTATGGGTGGTGGA+GGG - chr1.4:29829253-29829272 MS.gene064902:CDS 45.0%
!! TCCGGTGTTGTTCATATGGT+TGG - chr1.4:29828501-29828520 MS.gene064902:CDS 45.0%
!!! ACCGGAGTCATTGATTTTGC+TGG - chr1.4:29828477-29828496 MS.gene064902:CDS 45.0%
!!! GTCATTGATTTTGCTGGCTC+CGG - chr1.4:29828483-29828502 MS.gene064902:CDS 45.0%
!!! TAATTTCTTTTAAATTTTTA+TGG - chr1.4:29828826-29828845 MS.gene064902:CDS 5.0%
AAACAAAAGGTCCCCGGTGT+TGG + chr1.4:29828454-29828473 None:intergenic 50.0%
AACGTGACCGATGTCTGCAA+CGG - chr1.4:29828973-29828992 MS.gene064902:CDS 50.0%
AATCGCGTGCAACAAACTCG+CGG - chr1.4:29829089-29829108 MS.gene064902:CDS 50.0%
AGGTTCTACAACGGAACACC+CGG + chr1.4:29829028-29829047 None:intergenic 50.0%
ATGGATTTGACCCGACATGG+TGG - chr1.4:29829408-29829427 MS.gene064902:CDS 50.0%
CCACATACAATTGCTGCCCA+AGG + chr1.4:29829048-29829067 None:intergenic 50.0%
CCAGGTTTATCCGGGTAAAC+TGG - chr1.4:29829218-29829237 MS.gene064902:CDS 50.0%
CCAGTTTACCCGGATAAACC+TGG + chr1.4:29829221-29829240 None:intergenic 50.0%
CCTTGGGCAGCAATTGTATG+TGG - chr1.4:29829045-29829064 MS.gene064902:CDS 50.0%
GAAGCAGCACAATTACACGG+TGG - chr1.4:29829138-29829157 MS.gene064902:CDS 50.0%
GATGAAATTTGCGGCGGCTT+CGG + chr1.4:29827950-29827969 None:intergenic 50.0%
GGGTGGGTAAGTGCAACTAT+GGG - chr1.4:29829318-29829337 MS.gene064902:CDS 50.0%
GGTATGGATTTGACCCGACA+TGG - chr1.4:29829405-29829424 MS.gene064902:CDS 50.0%
GGTGCAGATGAAATTTGCGG+CGG + chr1.4:29827956-29827975 None:intergenic 50.0%
GGTGTTCCGTTGTAGAACCT+TGG - chr1.4:29829028-29829047 MS.gene064902:CDS 50.0%
GTAAACTGGGTCGACCCTAT+GGG - chr1.4:29829232-29829251 MS.gene064902:CDS 50.0%
TATCACCAGCGGCTCTATTG+CGG - chr1.4:29828320-29828339 MS.gene064902:CDS 50.0%
TGATCGAACCGTCCAATTCG+CGG + chr1.4:29828564-29828583 None:intergenic 50.0%
TGCTGCCCAAGGTTCTACAA+CGG + chr1.4:29829037-29829056 None:intergenic 50.0%
TGGATTTGACCCGACATGGT+GGG - chr1.4:29829409-29829428 MS.gene064902:CDS 50.0%
TGGGTGGGTAAGTGCAACTA+TGG - chr1.4:29829317-29829336 MS.gene064902:CDS 50.0%
TTGAAGGTCCGCGAATTGGA+CGG - chr1.4:29828553-29828572 MS.gene064902:intron 50.0%
! AACACCGGGGACCTTTTGTT+TGG - chr1.4:29828453-29828472 MS.gene064902:CDS 50.0%
! AGCTGCGAAACAGAGCATTC+CGG + chr1.4:29827895-29827914 None:intergenic 50.0%
! CACAGGGTTTGTTTACCCTG+TGG - chr1.4:29828386-29828405 MS.gene064902:CDS 50.0%
! GGGTTATTTATGGGTGGTGG+AGG - chr1.4:29829252-29829271 MS.gene064902:CDS 50.0%
! GGTTGGTATGGTTTCAACCC+TGG - chr1.4:29828657-29828676 MS.gene064902:intron 50.0%
! GGTTTTGCCATGCTTTGTGC+TGG - chr1.4:29828075-29828094 MS.gene064902:CDS 50.0%
! TGACTCCGGTGCCAAACAAA+AGG + chr1.4:29828467-29828486 None:intergenic 50.0%
!! GTTTTGGTCCGGTGATGGTT+GGG - chr1.4:29828419-29828438 MS.gene064902:CDS 50.0%
!! TGGCTCCGGTGTTGTTCATA+TGG - chr1.4:29828497-29828516 MS.gene064902:CDS 50.0%
!!! CATTGGTTTTGGTCCGGTGA+TGG - chr1.4:29828414-29828433 MS.gene064902:CDS 50.0%
ATCGGTCACGTTCCAGTGAC+CGG + chr1.4:29828965-29828984 None:intergenic 55.0%
GACGACATTAGCCGGTTGCA+CGG - chr1.4:29828903-29828922 MS.gene064902:CDS 55.0%
GCACAATTACACGGTGGGTG+TGG - chr1.4:29829144-29829163 MS.gene064902:CDS 55.0%
GGTAAACTGGGTCGACCCTA+TGG - chr1.4:29829231-29829250 MS.gene064902:CDS 55.0%
GGTGCCAAACAAAAGGTCCC+CGG + chr1.4:29828460-29828479 None:intergenic 55.0%
GTGCAACAAACTCGCGGAGA+AGG - chr1.4:29829095-29829114 MS.gene064902:CDS 55.0%
TAGGGTCGACCCAGTTTACC+CGG + chr1.4:29829231-29829250 None:intergenic 55.0%
TATGGGCAATGGAGTGCGGT+GGG - chr1.4:29828865-29828884 MS.gene064902:CDS 55.0%
TTATGGGCAATGGAGTGCGG+TGG - chr1.4:29828864-29828883 MS.gene064902:CDS 55.0%
TTCTACAACGGAACACCCGG+CGG + chr1.4:29829025-29829044 None:intergenic 55.0%
!! GCTTTTGCTATAGCAGCCGC+TGG - chr1.4:29828297-29828316 MS.gene064902:CDS 55.0%
!! GGGGACCTTTTGTTTGGCAC+CGG - chr1.4:29828459-29828478 MS.gene064902:CDS 55.0%
!! GGTTTTGGTCCGGTGATGGT+TGG - chr1.4:29828418-29828437 MS.gene064902:CDS 55.0%
!! GTCTGCAACGGATTACTCGG+CGG - chr1.4:29828985-29829004 MS.gene064902:CDS 55.0%
AGAGCCGCTGGTGATACCAG+CGG + chr1.4:29828316-29828335 None:intergenic 60.0%
ATGGGCAATGGAGTGCGGTG+GGG - chr1.4:29828866-29828885 MS.gene064902:CDS 60.0%
CGATCATTCTGGTCGAGCCG+TGG - chr1.4:29828578-29828597 MS.gene064902:intron 60.0%
CGTTAGAGCTGCCGTGCAAC+CGG + chr1.4:29828917-29828936 None:intergenic 60.0%
GCGGTGACGACGACATTAGC+CGG - chr1.4:29828895-29828914 MS.gene064902:CDS 60.0%
GGTTTCGCTGCGATAACCGC+CGG - chr1.4:29829006-29829025 MS.gene064902:CDS 60.0%
GTTTCGCTGCGATAACCGCC+GGG - chr1.4:29829007-29829026 MS.gene064902:CDS 60.0%
TCGCAGCTCGCGCAGTTCAT+CGG - chr1.4:29827907-29827926 MS.gene064902:CDS 60.0%
TCGTTCCGCAATAGAGCCGC+TGG + chr1.4:29828328-29828347 None:intergenic 60.0%
TGCGCTAGCCCAACCATCAC+CGG + chr1.4:29828430-29828449 None:intergenic 60.0%
! ACTGTGTCCGCGAAGTGCCA+CGG + chr1.4:29828598-29828617 None:intergenic 60.0%
!! TGGGCTAGCGCAACCAACAC+CGG - chr1.4:29828438-29828457 MS.gene064902:CDS 60.0%
AACGTCCTCGACGCTGCTGC+TGG - chr1.4:29828138-29828157 MS.gene064902:CDS 65.0%
ATGGAGTGCGGTGGGGAGGA+CGG - chr1.4:29828873-29828892 MS.gene064902:CDS 65.0%
ATTTGCGGCGGCTTCGGCGT+TGG + chr1.4:29827944-29827963 None:intergenic 65.0%
GCAGCCGCTGGTATCACCAG+CGG - chr1.4:29828309-29828328 MS.gene064902:CDS 65.0%
GGCTAGCGCAACCAACACCG+GGG - chr1.4:29828440-29828459 MS.gene064902:CDS 65.0%
! AGCAGCAGCGTCGAGGACGT+TGG + chr1.4:29828139-29828158 None:intergenic 65.0%
! GGGCTAGCGCAACCAACACC+GGG - chr1.4:29828439-29828458 MS.gene064902:CDS 65.0%
!! CTTCGGCGTTGGTGGCGTTG+GGG + chr1.4:29827933-29827952 None:intergenic 65.0%
!! GCTTCGGCGTTGGTGGCGTT+GGG + chr1.4:29827934-29827953 None:intergenic 65.0%
ACACGGTGGGTGTGGCGCGT+GGG - chr1.4:29829152-29829171 MS.gene064902:CDS 70.0%
GGCAATGGAGTGCGGTGGGG+AGG - chr1.4:29828869-29828888 MS.gene064902:CDS 70.0%
GGTCGAGCCGTGGCACTTCG+CGG - chr1.4:29828588-29828607 MS.gene064902:intron 70.0%
GTCCTCGACGCTGCTGCTGG+TGG - chr1.4:29828141-29828160 MS.gene064902:CDS 70.0%
TACACGGTGGGTGTGGCGCG+TGG - chr1.4:29829151-29829170 MS.gene064902:CDS 70.0%
!! GGCTTCGGCGTTGGTGGCGT+TGG + chr1.4:29827935-29827954 None:intergenic 70.0%
CACGGTGGGTGTGGCGCGTG+GGG - chr1.4:29829153-29829172 MS.gene064902:CDS 75.0%
CGCCACCAGCAGCAGCGTCG+AGG + chr1.4:29828146-29828165 None:intergenic 75.0%
GAGTGCGGTGGGGAGGACGG+CGG - chr1.4:29828876-29828895 MS.gene064902:CDS 75.0%
! TGCGGCGGCTTCGGCGTTGG+TGG + chr1.4:29827941-29827960 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 29827883 29829536 29827883 ID=MS.gene064902
chr1.4 mRNA 29827883 29829536 29827883 ID=MS.gene064902.t1;Parent=MS.gene064902
chr1.4 exon 29828705 29829536 29828705 ID=MS.gene064902.t1.exon1;Parent=MS.gene064902.t1
chr1.4 CDS 29828705 29829536 29828705 ID=cds.MS.gene064902.t1;Parent=MS.gene064902.t1
chr1.4 exon 29827883 29828562 29827883 ID=MS.gene064902.t1.exon2;Parent=MS.gene064902.t1
chr1.4 CDS 29827883 29828562 29827883 ID=cds.MS.gene064902.t1;Parent=MS.gene064902.t1
Gene Sequence

>MS.gene064902

ATGTCGCTGCCGGAATGCTCTGTTTCGCAGCTCGCGCAGTTCATCGGCCCCAACGCCACCAACGCCGAAGCCGCCGCAAATTTCATCTGCACCCAGTTCACAGCAGTGTCAAACAGATTCGTCGACACACAATTCGCTGTTGACAACACCTATCTTCTATTCTCAGCTTACCTTGTTTTCTCTATGCAACTTGGTTTTGCCATGCTTTGTGCTGGTTCAGTTCGTGCTAAGAACACCATGAATATCATGCTTACCAACGTCCTCGACGCTGCTGCTGGTGGCGTTTTCTACTATCTCTTCGGTTTTGCCTTTGCTTTTGGAACACCTTCAAATGGTTTCATTGGAAAACATTTCTTTGGTCTGAAGGATGTTCCGACTGAAAATTTTGATTACAGTTATTTTCTCTATCAATGGGCTTTTGCTATAGCAGCCGCTGGTATCACCAGCGGCTCTATTGCGGAACGAACTCAGTTCGTTGCGTATCTTATTTACTCATCGTTTCTCACAGGGTTTGTTTACCCTGTGGTTTCTCATTGGTTTTGGTCCGGTGATGGTTGGGCTAGCGCAACCAACACCGGGGACCTTTTGTTTGGCACCGGAGTCATTGATTTTGCTGGCTCCGGTGTTGTTCATATGGTTGGTGGTATTGCTGGTTTATGGGGAGCACTTATTGAAGGTCCGCGAATTGGACGGTTCGATCATTCTGGTCGAGCCGTGGCACTTCGCGGACACAGTGCCTCTCTTGTTGTTCTTGGCACTTTCATGCTTTGGTTTGGTTGGTATGGTTTCAACCCTGGTTCCTTCACCAAGATTCTCAGTCCTTATAATTCAGGTAGTTAACTATCTTACTTTTTGTACTAACTACTTTCATTGTTTTGGATGCACCTAATGGAAAGCTCTTGTTGAAAAATAATATTTACTTTTTCTTCTCCTTTTCAAATTTTAATTTCTTTTAAATTTTTATGGAATTTTAGGTACTTATTATGGGCAATGGAGTGCGGTGGGGAGGACGGCGGTGACGACGACATTAGCCGGTTGCACGGCAGCTCTAACGACACTTTTCGGGAAAAGAATTCTCTCCGGTCACTGGAACGTGACCGATGTCTGCAACGGATTACTCGGCGGTTTCGCTGCGATAACCGCCGGGTGTTCCGTTGTAGAACCTTGGGCAGCAATTGTATGTGGCTTCATCGCCGCTATTGTTTTAATCGCGTGCAACAAACTCGCGGAGAAGGTGCGTTACGATGATCCATTAGAAGCAGCACAATTACACGGTGGGTGTGGCGCGTGGGGAATAATATTCACCGCTTTGTTTGCAAAAGAAGAGTATGTGAACCAGGTTTATCCGGGTAAACTGGGTCGACCCTATGGGTTATTTATGGGTGGTGGAGGGAAATTATTAGGGGCACATGTGATACAAATTTTGGTTATTATTGGGTGGGTAAGTGCAACTATGGGTCCACTATTCTTTGTTTTGAATAAATTGAAGTTGTTGAGGATTTCAACTGAAGATGAACTTGCGGGTATGGATTTGACCCGACATGGTGGGTTTGCTTATGCTTATGAAGATGAAGGATCACATAGAAATGGGACTCAATTGAGGAAAATTGATCATAATTCTTCTTCCACTCCTACTCCAACTACAGATTTGTGA
Protein sequence

>MS.gene064902.t1

MSLPECSVSQLAQFIGPNATNAEAAANFICTQFTAVSNRFVDTQFAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGVFYYLFGFAFAFGTPSNGFIGKHFFGLKDVPTENFDYSYFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFVYPVVSHWFWSGDGWASATNTGDLLFGTGVIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDHSGRAVALRGHSASLVVLGTFMLWFGWYGFNPGSFTKILSPYNSGTYYGQWSAVGRTAVTTTLAGCTAALTTLFGKRILSGHWNVTDVCNGLLGGFAAITAGCSVVEPWAAIVCGFIAAIVLIACNKLAEKVRYDDPLEAAQLHGGCGAWGIIFTALFAKEEYVNQVYPGKLGRPYGLFMGGGGKLLGAHVIQILVIIGWVSATMGPLFFVLNKLKLLRISTEDELAGMDLTRHGGFAYAYEDEGSHRNGTQLRKIDHNSSSTPTPTTDL