AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065102

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065102.t1 MTR_1g111350 94.748 457 20 1 15 471 15 467 0.0 838
MS.gene065102.t1 MTR_6g085010 55.876 485 191 6 5 471 12 491 3.61e-168 483
MS.gene065102.t1 MTR_7g117430 56.098 410 168 6 73 471 76 484 6.22e-142 416
MS.gene065102.t1 MTR_1g115210 45.279 466 235 7 21 471 26 486 4.69e-138 406
MS.gene065102.t1 MTR_1g039270 45.578 441 227 4 44 471 29 469 1.29e-126 376
MS.gene065102.t1 MTR_8g103880 42.334 437 221 6 31 464 40 448 1.21e-112 340
MS.gene065102.t1 MTR_3g097150 40.235 425 230 11 68 471 25 446 3.46e-87 274
MS.gene065102.t1 MTR_3g046300 38.542 384 197 9 120 471 82 458 1.73e-73 239
MS.gene065102.t1 MTR_5g091130 38.583 381 202 9 120 471 176 553 2.80e-73 241
MS.gene065102.t1 MTR_4g007740 34.735 452 253 12 53 471 35 477 3.31e-68 226
MS.gene065102.t1 MTR_3g053710 36.211 417 225 14 76 471 70 466 7.31e-67 222
MS.gene065102.t1 MTR_2g028190 33.566 429 246 14 74 471 31 451 8.82e-63 211
MS.gene065102.t1 MTR_5g080890 36.082 388 220 15 101 471 55 431 4.50e-61 206
MS.gene065102.t1 MTR_5g080960 36.340 388 219 15 101 471 55 431 2.58e-60 204
MS.gene065102.t1 MTR_3g053980 35.644 404 223 10 98 471 89 485 5.78e-60 204
MS.gene065102.t1 MTR_5g044770 36.056 355 199 13 134 471 85 428 1.69e-59 201
MS.gene065102.t1 MTR_8g096990 31.340 418 246 13 86 471 21 429 4.66e-59 200
MS.gene065102.t1 MTR_8g038280 33.254 421 243 16 74 471 28 433 6.09e-58 197
MS.gene065102.t1 MTR_0036s0110 32.938 422 222 19 80 471 50 440 3.53e-55 190
MS.gene065102.t1 MTR_0036s0070 33.333 411 237 15 78 471 34 424 6.15e-55 189
MS.gene065102.t1 MTR_5g008110 36.313 358 198 13 134 471 66 413 1.01e-54 188
MS.gene065102.t1 MTR_5g008150 35.854 357 202 11 134 471 62 410 1.28e-53 185
MS.gene065102.t1 MTR_5g038360 32.195 410 247 14 84 471 34 434 1.10e-52 184
MS.gene065102.t1 MTR_2g059490 35.097 359 203 14 135 471 58 408 1.29e-51 181
MS.gene065102.t1 MTR_2g059460 34.831 356 203 12 135 471 59 404 2.23e-51 179
MS.gene065102.t1 MTR_5g008070 35.593 354 205 10 133 471 79 424 1.75e-50 177
MS.gene065102.t1 MTR_8g038250 32.220 419 225 15 86 471 21 413 2.35e-50 177
MS.gene065102.t1 MTR_3g028120 30.193 414 260 12 78 471 32 436 2.57e-50 177
MS.gene065102.t1 MTR_2g055840 32.961 358 212 9 140 471 18 373 5.38e-50 175
MS.gene065102.t1 MTR_0036s0050 32.553 427 228 17 78 471 34 433 4.35e-47 169
MS.gene065102.t1 MTR_0024s0040 32.527 372 228 11 116 471 44 408 5.59e-47 167
MS.gene065102.t1 MTR_8g038310 29.557 406 255 12 74 471 29 411 1.04e-46 167
MS.gene065102.t1 MTR_5g044570 33.711 353 193 13 131 471 86 409 1.05e-45 164
MS.gene065102.t1 MTR_6g090290 32.891 377 227 10 114 471 65 434 4.29e-45 163
MS.gene065102.t1 MTR_2g067830 30.874 366 208 15 134 471 56 404 1.62e-43 158
MS.gene065102.t1 MTR_1g037630 33.333 363 213 9 124 471 80 428 1.80e-42 156
MS.gene065102.t1 MTR_3g014290 33.058 363 214 9 124 471 68 416 1.83e-42 155
MS.gene065102.t1 MTR_8g039540 32.951 349 215 10 133 471 94 433 7.45e-42 154
MS.gene065102.t1 MTR_4g109760 33.711 353 205 13 136 471 56 396 1.77e-40 150
MS.gene065102.t1 MTR_3g100500 32.405 395 242 10 90 471 55 437 1.73e-38 145
MS.gene065102.t1 MTR_2g070430 28.864 440 264 14 71 470 22 452 6.20e-38 144
MS.gene065102.t1 MTR_7g105850 29.820 389 218 13 130 471 85 465 8.17e-38 144
MS.gene065102.t1 MTR_1g060750 29.208 404 223 18 94 471 8 374 1.02e-37 142
MS.gene065102.t1 MTR_2g105300 30.588 340 197 15 154 468 54 379 4.29e-37 140
MS.gene065102.t1 MTR_5g077850 31.268 355 214 12 142 471 88 437 4.89e-37 141
MS.gene065102.t1 MTR_2g063110 28.638 426 235 16 104 471 94 508 5.18e-37 142
MS.gene065102.t1 MTR_3g068000 31.551 374 204 15 140 471 78 441 9.73e-37 141
MS.gene065102.t1 MTR_4g045857 34.800 250 137 9 116 345 43 286 9.32e-36 134
MS.gene065102.t1 MTR_5g012490 29.592 392 204 14 145 471 85 469 3.63e-35 137
MS.gene065102.t1 MTR_6g088760 28.680 394 230 15 86 438 21 404 6.22e-35 137
MS.gene065102.t1 MTR_3g065160 30.748 361 208 14 140 471 79 426 8.30e-35 135
MS.gene065102.t1 MTR_4g100990 28.640 419 220 16 120 471 64 470 6.83e-34 133
MS.gene065102.t1 MTR_7g062360 30.997 371 208 16 140 471 64 425 1.46e-32 129
MS.gene065102.t1 MTR_4g056420 28.495 372 223 14 133 471 75 436 1.03e-30 124
MS.gene065102.t1 MTR_5g077920 29.949 394 242 12 107 471 38 426 8.44e-30 121
MS.gene065102.t1 MTR_7g102750 28.533 368 226 12 134 471 80 440 6.42e-29 119
MS.gene065102.t1 MTR_5g008090 33.224 304 170 13 190 471 20 312 2.90e-28 114
MS.gene065102.t1 MTR_2g084550 28.763 372 221 14 134 471 76 437 1.02e-26 112
MS.gene065102.t1 MTR_1g078240 26.579 380 234 14 127 471 61 430 1.27e-26 112
MS.gene065102.t1 MTR_7g080230 27.937 383 223 17 127 471 63 430 1.45e-26 112
MS.gene065102.t1 MTR_1g078240 26.121 379 236 14 127 471 61 429 1.86e-25 109
MS.gene065102.t1 MTR_4g095270 28.889 360 221 14 133 471 78 423 2.27e-25 109
MS.gene065102.t1 MTR_5g016260 27.451 357 230 11 133 471 86 431 2.42e-25 109
MS.gene065102.t1 MTR_4g095270 28.889 360 221 14 133 471 78 423 2.68e-25 109
MS.gene065102.t1 MTR_5g016260 27.451 357 230 11 133 471 86 431 2.97e-25 109
MS.gene065102.t1 MTR_1g061940 26.613 372 234 12 132 471 80 444 5.78e-25 107
MS.gene065102.t1 MTR_7g102750 25.935 401 254 12 76 446 28 415 6.79e-25 107
MS.gene065102.t1 MTR_8g075090 28.184 369 210 17 136 471 113 459 7.68e-24 104
MS.gene065102.t1 MTR_8g075090 28.184 369 210 17 136 471 113 459 8.06e-24 104
MS.gene065102.t1 MTR_8g075010 26.486 370 217 12 136 471 101 449 1.34e-23 103
MS.gene065102.t1 MTR_5g022880 27.056 377 206 19 136 471 113 461 4.12e-22 99.8
MS.gene065102.t1 MTR_1g078240 27.469 324 198 12 127 422 61 375 2.12e-21 96.3
MS.gene065102.t1 MTR_7g021510 24.611 386 234 14 127 471 64 433 2.14e-21 97.1
MS.gene065102.t1 MTR_6g084710 26.316 380 235 17 126 471 182 550 6.56e-21 95.9
MS.gene065102.t1 MTR_8g075090 29.293 297 161 14 136 402 113 390 2.10e-20 94.0
MS.gene065102.t1 MTR_4g023650 27.273 385 224 18 126 471 52 419 9.10e-20 91.7
MS.gene065102.t1 MTR_2g022450 23.171 410 246 13 111 467 18 411 4.21e-19 89.7
MS.gene065102.t1 MTR_1g072450 25.672 409 230 19 114 467 26 415 4.25e-19 89.7
MS.gene065102.t1 MTR_3g087780 28.226 372 210 17 136 471 100 450 8.91e-19 89.4
MS.gene065102.t1 MTR_6g084710 28.794 257 155 11 126 364 182 428 1.21e-18 89.0
MS.gene065102.t1 MTR_1g072380 23.389 419 238 16 112 467 34 432 1.21e-18 88.6
MS.gene065102.t1 MTR_5g061690 27.624 362 222 14 136 471 102 449 1.73e-18 88.6
MS.gene065102.t1 MTR_1g029810 27.100 369 223 17 134 471 56 409 2.79e-18 87.0
MS.gene065102.t1 MTR_1g029810 27.100 369 223 17 134 471 56 409 6.35e-18 86.3
MS.gene065102.t1 MTR_2g022320 24.611 386 220 13 136 467 37 405 1.26e-16 82.0
MS.gene065102.t1 MTR_1g072420 24.697 413 230 18 114 467 26 416 1.33e-16 82.0
MS.gene065102.t1 MTR_4g023630 25.393 382 235 17 126 471 52 419 2.01e-16 81.6
MS.gene065102.t1 MTR_5g080330 27.114 343 207 15 151 471 1 322 4.95e-15 77.4
MS.gene065102.t1 MTR_2g022380 25.000 380 210 15 144 467 49 409 1.20e-14 76.3
MS.gene065102.t1 MTR_2g022430 24.297 391 217 16 136 467 40 410 5.86e-14 73.9
MS.gene065102.t1 MTR_7g080230 26.829 328 192 16 177 471 48 360 1.98e-13 72.4
MS.gene065102.t1 MTR_2g022370 23.896 385 221 15 136 467 40 405 2.35e-13 72.0
MS.gene065102.t1 MTR_3g087780 27.469 324 181 14 178 471 9 308 2.93e-13 71.6
MS.gene065102.t1 MTR_3g006820 22.249 418 258 17 92 471 24 412 1.85e-11 66.2
MS.gene065102.t1 MTR_2g022480 23.810 378 216 17 136 458 50 410 3.36e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065102.t1 AT1G25510 61.783 471 150 5 22 471 22 483 0.0 575
MS.gene065102.t1 AT3G18490 54.825 487 194 7 10 471 15 500 4.08e-176 503
MS.gene065102.t1 AT1G01300 55.072 414 169 7 74 471 72 484 2.34e-136 402
MS.gene065102.t1 AT3G61820 54.135 399 158 9 97 471 86 483 5.27e-127 378
MS.gene065102.t1 AT3G20015 48.077 416 204 3 68 471 55 470 7.18e-122 364
MS.gene065102.t1 AT5G10770 37.619 420 218 12 87 471 63 473 1.67e-78 253
MS.gene065102.t1 AT2G03200 38.251 366 204 10 125 471 96 458 6.83e-73 238
MS.gene065102.t1 AT3G59080 35.142 424 240 11 78 471 114 532 2.26e-69 230
MS.gene065102.t1 AT5G10760 38.971 408 226 11 76 471 68 464 2.55e-68 226
MS.gene065102.t1 AT2G42980 33.843 458 257 14 51 471 76 524 7.80e-67 223
MS.gene065102.t1 AT3G59080 33.732 418 218 10 78 471 114 496 4.73e-60 205
MS.gene065102.t1 AT3G25700 32.558 430 247 15 82 471 23 449 4.18e-58 198
MS.gene065102.t1 AT5G33340 36.816 402 224 16 91 471 42 434 2.05e-56 193
MS.gene065102.t1 AT1G79720 31.849 449 254 12 64 471 45 482 1.18e-55 192
MS.gene065102.t1 AT1G64830 35.775 355 201 12 134 471 84 428 4.41e-55 190
MS.gene065102.t1 AT3G54400 35.246 366 213 12 119 471 70 424 2.32e-54 187
MS.gene065102.t1 AT1G09750 32.337 368 228 11 119 471 87 448 4.16e-46 166
MS.gene065102.t1 AT1G31450 32.949 434 231 19 85 471 21 441 4.22e-46 166
MS.gene065102.t1 AT2G35615 31.954 435 243 16 83 471 16 443 1.63e-45 164
MS.gene065102.t1 AT2G28040 31.897 348 205 8 135 471 55 381 3.25e-43 157
MS.gene065102.t1 AT2G28040 31.897 348 205 8 135 471 64 390 4.59e-43 157
MS.gene065102.t1 AT2G28010 29.830 352 206 8 136 471 65 391 2.05e-42 155
MS.gene065102.t1 AT5G07030 28.049 410 249 12 85 471 68 454 4.25e-41 153
MS.gene065102.t1 AT2G28220 30.000 350 212 9 136 471 67 397 3.90e-39 146
MS.gene065102.t1 AT2G28225 30.595 353 206 10 136 471 67 397 9.06e-39 145
MS.gene065102.t1 AT3G12700 28.855 454 277 16 55 471 16 460 1.03e-38 146
MS.gene065102.t1 AT2G28030 30.114 352 205 11 136 471 61 387 1.88e-38 144
MS.gene065102.t1 AT3G52500 29.306 389 225 13 129 471 83 467 2.29e-38 145
MS.gene065102.t1 AT4G30030 30.398 352 219 13 136 471 78 419 1.86e-37 142
MS.gene065102.t1 AT3G02740 27.211 441 264 15 63 471 24 439 1.26e-36 141
MS.gene065102.t1 AT3G02740 29.348 368 215 14 136 471 10 364 2.32e-36 139
MS.gene065102.t1 AT5G37540 32.123 358 210 13 142 471 86 438 1.02e-34 135
MS.gene065102.t1 AT5G36260 27.568 370 224 14 134 471 76 433 2.20e-34 135
MS.gene065102.t1 AT2G39710 29.459 370 211 14 140 469 69 428 2.61e-34 134
MS.gene065102.t1 AT4G30040 30.966 352 213 13 136 466 85 427 5.30e-34 132
MS.gene065102.t1 AT2G23945 26.824 425 248 18 72 459 38 436 8.64e-34 132
MS.gene065102.t1 AT5G02190 30.352 369 207 15 145 471 82 442 2.26e-31 125
MS.gene065102.t1 AT5G43100 27.577 359 226 12 133 471 73 417 7.25e-31 126
MS.gene065102.t1 AT1G66180 30.110 362 212 14 140 471 76 426 9.07e-31 124
MS.gene065102.t1 AT5G43100 27.577 359 226 12 133 471 73 417 1.00e-30 124
MS.gene065102.t1 AT2G36670 27.520 367 230 12 134 471 98 457 1.55e-29 121
MS.gene065102.t1 AT2G36670 27.273 385 243 13 116 471 86 462 2.07e-29 121
MS.gene065102.t1 AT2G36670 27.397 365 229 12 136 471 34 391 3.00e-29 119
MS.gene065102.t1 AT2G36670 27.397 365 229 12 136 471 34 391 3.00e-29 119
MS.gene065102.t1 AT1G65240 26.216 370 226 16 134 471 72 426 2.39e-28 117
MS.gene065102.t1 AT5G45120 26.866 402 222 15 136 471 83 478 1.74e-27 115
MS.gene065102.t1 AT3G50050 27.654 358 229 11 133 471 90 436 3.68e-27 115
MS.gene065102.t1 AT1G05840 27.404 416 236 18 104 471 41 438 3.87e-27 114
MS.gene065102.t1 AT5G22850 26.812 414 254 16 93 471 41 440 5.00e-26 110
MS.gene065102.t1 AT2G17760 28.022 364 216 15 136 471 104 449 2.61e-25 108
MS.gene065102.t1 AT3G51330 26.575 365 226 14 136 471 102 453 8.96e-25 107
MS.gene065102.t1 AT4G12920 26.525 377 229 12 116 471 38 387 1.21e-24 105
MS.gene065102.t1 AT3G51330 26.575 365 226 14 136 471 102 453 1.49e-24 107
MS.gene065102.t1 AT1G08210 27.027 370 227 15 134 471 130 488 6.97e-23 102
MS.gene065102.t1 AT1G08210 27.027 370 227 15 134 471 116 474 7.96e-23 101
MS.gene065102.t1 AT1G08210 27.027 370 227 15 134 471 82 440 9.59e-23 101
MS.gene065102.t1 AT1G77480 26.440 382 226 17 126 471 57 419 1.19e-22 100
MS.gene065102.t1 AT1G77480 26.440 382 226 17 126 471 57 419 2.45e-22 99.8
MS.gene065102.t1 AT3G51350 26.027 365 227 13 136 471 102 452 4.75e-22 99.4
MS.gene065102.t1 AT4G33490 26.494 385 221 18 126 471 50 411 6.50e-22 98.2
MS.gene065102.t1 AT3G25700 26.885 305 166 11 82 331 23 325 1.74e-21 95.9
MS.gene065102.t1 AT3G25700 36.296 135 77 6 343 471 216 347 6.58e-13 70.5
MS.gene065102.t1 AT3G51340 25.549 364 224 14 136 471 103 447 2.33e-21 97.4
MS.gene065102.t1 AT3G51330 26.686 341 208 14 136 447 102 429 6.75e-21 95.5
MS.gene065102.t1 AT3G42550 25.000 352 214 11 133 471 75 389 2.87e-20 93.2
MS.gene065102.t1 AT1G08210 27.928 333 198 14 134 435 82 403 4.62e-20 93.2
MS.gene065102.t1 AT4G35880 24.377 361 232 11 136 471 107 451 1.49e-19 91.7
MS.gene065102.t1 AT4G16563 27.889 398 202 16 153 471 100 491 1.29e-18 89.0
MS.gene065102.t1 AT4G33490 28.920 287 156 14 126 386 47 311 1.76e-18 87.8
MS.gene065102.t1 AT5G10080 25.067 375 221 14 136 471 100 453 2.39e-18 88.2
MS.gene065102.t1 AT1G49050 22.400 375 243 18 136 471 30 395 4.71e-18 86.7
MS.gene065102.t1 AT3G51340 25.085 295 181 10 136 404 103 383 9.52e-17 82.4
MS.gene065102.t1 AT3G51340 23.592 373 232 14 136 471 103 459 1.71e-16 82.4
MS.gene065102.t1 AT3G51360 23.744 438 282 16 68 471 26 445 4.12e-16 81.3
MS.gene065102.t1 AT3G51360 23.744 438 282 16 68 471 16 435 5.41e-16 80.5
MS.gene065102.t1 AT3G51340 25.524 286 173 10 136 395 103 374 5.57e-16 80.1
MS.gene065102.t1 AT3G51360 24.309 362 233 13 136 471 5 351 2.97e-15 77.8
MS.gene065102.t1 AT5G48430 32.121 165 104 4 307 467 229 389 4.67e-15 77.4
MS.gene065102.t1 AT3G51360 24.455 413 260 16 68 446 26 420 5.20e-14 74.3
MS.gene065102.t1 AT3G51360 24.455 413 260 16 68 446 16 410 5.83e-14 73.9
MS.gene065102.t1 AT3G51360 24.800 375 233 14 68 411 26 382 1.04e-13 73.2
MS.gene065102.t1 AT3G51360 24.800 375 233 14 68 411 16 372 1.06e-13 73.2
MS.gene065102.t1 AT3G51360 24.620 329 201 12 68 367 26 336 6.18e-11 64.3

Find 129 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAACACAGAGAAACTTTC+TGG 0.178463 1.2:-82454696 MS.gene065102:CDS
TGATGCAGTTGGCTCGAAAA+TGG 0.248018 1.2:+82454515 None:intergenic
TTGATTTAACTTAGTTGTTA+TGG 0.248364 1.2:+82454722 None:intergenic
GTTGCTGTTGTTTAAAGTTT+TGG 0.252744 1.2:+82454892 None:intergenic
TTGACTTGTTCCGGAGATAA+TGG 0.270765 1.2:+82454671 None:intergenic
TGGCTCACCGATTCCGATTC+TGG 0.285369 1.2:+82454629 None:intergenic
GGCTTGTTCGTTGGTGCAGC+TGG 0.291445 1.2:-82454294 MS.gene065102:CDS
GCAATTAATTAGCAACTGTT+AGG 0.292112 1.2:+82453640 None:intergenic
AGTTACCATCGGCGTTAATA+AGG 0.302292 1.2:-82454355 MS.gene065102:CDS
AACATCAAGTAGTGTGGTTT+TGG 0.306517 1.2:+82454956 None:intergenic
ATCACTTCTTTGTCGATTAT+CGG 0.311520 1.2:-82453727 MS.gene065102:CDS
CACAATAATCCCTCCATTCC+CGG 0.318603 1.2:+82454014 None:intergenic
GTTGGTGCAGCTGGCTTAAT+CGG 0.352535 1.2:-82454285 MS.gene065102:CDS
CCGACGGTGGCGTTTCATTT+CGG 0.354586 1.2:-82453838 MS.gene065102:CDS
GACCTTGCAAATTCATTAGT+TGG 0.361959 1.2:-82453670 MS.gene065102:CDS
GGAGGGATTATTGTGGATTC+CGG 0.378793 1.2:-82454006 MS.gene065102:CDS
ATGTTCCGAGAATGTGCTAA+TGG 0.381944 1.2:+82454990 None:intergenic
GCCGCTACCTTGACTTGTTC+CGG 0.382831 1.2:+82454662 None:intergenic
GTTCGTGATGCGTTTGTTAA+GGG 0.397061 1.2:-82453943 MS.gene065102:CDS
GGCTGAGTCGCGGTCGAGTC+GGG 0.408483 1.2:+82454755 None:intergenic
CGGCGAGTTGCTTGCAATAC+CGG 0.408895 1.2:-82454061 MS.gene065102:CDS
TGACTTGTTCCGGAGATAAT+GGG 0.411902 1.2:+82454672 None:intergenic
ATCGGCTTGGCGGTAGCAAT+CGG 0.415933 1.2:+82454539 None:intergenic
TACCATTGCGACACTGAGAT+TGG 0.416758 1.2:+82454442 None:intergenic
GGTTCGTGATGCGTTTGTTA+AGG 0.425062 1.2:-82453944 MS.gene065102:CDS
TCACTTCTTTGTCGATTATC+GGG 0.427363 1.2:-82453726 MS.gene065102:CDS
GAAAATGAAGAGGATGAAGT+TGG 0.428777 1.2:+82455025 None:intergenic
ACGCCGTTACCGCGCCGTTA+CGG 0.430716 1.2:-82454140 MS.gene065102:CDS
TTTCGGAGAAGGGAAAGTTT+TGG 0.431549 1.2:-82453821 MS.gene065102:CDS
TGTTCCGAGAATGTGCTAAT+GGG 0.432218 1.2:+82454991 None:intergenic
GAGGTGGATTCCGCCGGGAA+TGG 0.433765 1.2:-82454027 MS.gene065102:CDS
TTGGCGGTAGCAATCGGCGC+AGG 0.438339 1.2:+82454545 None:intergenic
ACACTGGAAGCGACATAAGC+TGG 0.442713 1.2:-82454581 MS.gene065102:CDS
GATGCAGTTGGCTCGAAAAT+GGG 0.447920 1.2:+82454516 None:intergenic
CAATTAATTAGCAACTGTTA+GGG 0.451342 1.2:+82453641 None:intergenic
TTACCTGGTACCGGTTGATT+CGG 0.452296 1.2:-82453782 MS.gene065102:CDS
TTTCCCCATTAGCACATTCT+CGG 0.461537 1.2:-82454995 MS.gene065102:CDS
GGCTTAATCGGCTTAGGCGG+TGG 0.470335 1.2:-82454273 MS.gene065102:CDS
TGTAATCAGCATGTGAAGAA+AGG 0.480027 1.2:+82454793 None:intergenic
GTTACGGCGTAATCCGCAAT+TGG 0.482972 1.2:-82454124 MS.gene065102:CDS
GGGCTGAGTCGCGGTCGAGT+CGG 0.486283 1.2:+82454754 None:intergenic
GCAGCTGGCTTAATCGGCTT+AGG 0.488301 1.2:-82454279 MS.gene065102:CDS
GGCGAGTATTTCTCCAGAAT+CGG 0.492819 1.2:-82454642 MS.gene065102:CDS
CTGGTACCGGTTGATTCGGT+AGG 0.494194 1.2:-82453778 MS.gene065102:CDS
GTAATCAGCATGTGAAGAAA+GGG 0.507550 1.2:+82454794 None:intergenic
TGGCGTTTCATTTCGGAGAA+GGG 0.507830 1.2:-82453831 MS.gene065102:CDS
TATTACTATGTGGGACTGGT+AGG 0.508043 1.2:-82454096 MS.gene065102:CDS
GCATACATGGTTCTCGACAC+TGG 0.512255 1.2:-82454597 MS.gene065102:CDS
GTGGCGTTTCATTTCGGAGA+AGG 0.515188 1.2:-82453832 MS.gene065102:CDS
GTGGATTCCGCCGGGAATGG+AGG 0.519178 1.2:-82454024 MS.gene065102:CDS
AAAGTCCCTACCGAATCAAC+CGG 0.525956 1.2:+82453772 None:intergenic
CTTTCTGGACCCATTATCTC+CGG 0.526305 1.2:-82454681 MS.gene065102:CDS
AAGGTTAAGAATGTAGCGCT+AGG 0.528751 1.2:-82454336 MS.gene065102:CDS
CAACCGTGTCACCGCCGTGC+CGG 0.529136 1.2:+82453987 None:intergenic
TATCAAGTCTCCTACGGTGA+TGG 0.529585 1.2:-82454411 MS.gene065102:CDS
CTTAACCTTATTAACGCCGA+TGG 0.530526 1.2:+82454350 None:intergenic
GGCGAAGAATTACCTGGTAC+CGG 0.532948 1.2:-82453791 MS.gene065102:CDS
AGTGGACCGTGATTCTGACT+TGG 0.537420 1.2:-82454199 MS.gene065102:CDS
TGGATTCCGCCGGGAATGGA+GGG 0.538719 1.2:-82454023 MS.gene065102:CDS
CGAAAATGGGATCGGCTTGG+CGG 0.539880 1.2:+82454529 None:intergenic
ATCGACAAAGAAGTGATCGT+TGG 0.539947 1.2:+82453733 None:intergenic
TCCACCTCAAAACTCGTCTC+CGG 0.540119 1.2:+82454042 None:intergenic
AGTTGTTATGGATTTGACTC+GGG 0.540361 1.2:+82454734 None:intergenic
CTACCGAATCAACCGGTACC+AGG 0.541898 1.2:+82453779 None:intergenic
TGGCGGTAGCAATCGGCGCA+GGG 0.543315 1.2:+82454546 None:intergenic
AACGACACGTCATCCGTCGC+GGG 0.546298 1.2:+82453907 None:intergenic
GACGTCGAATTGAGCTGTGC+CGG 0.546577 1.2:+82454237 None:intergenic
TTCGTGATGCGTTTGTTAAG+GGG 0.547844 1.2:-82453942 MS.gene065102:CDS
ACGCCGTAACGGCGCGGTAA+CGG 0.550364 1.2:+82454137 None:intergenic
GTAACGGCGTCACGAGAAAA+CGG 0.553971 1.2:+82454153 None:intergenic
AGCTGTGCCGGAAAAGAAAG+TGG 0.554624 1.2:+82454249 None:intergenic
AAGAATGTAGCGCTAGGGTG+TGG 0.556353 1.2:-82454330 MS.gene065102:CDS
CACTGGAAGCGACATAAGCT+GGG 0.556372 1.2:-82454580 MS.gene065102:CDS
ATTCCGGCACGGCGGTGACA+CGG 0.556428 1.2:-82453990 MS.gene065102:CDS
TATTTCTCCAGAATCGGAAT+CGG 0.558867 1.2:-82454636 MS.gene065102:CDS
AACAACGAAGGCTTGTTCGT+TGG 0.564849 1.2:-82454303 MS.gene065102:CDS
TAGTTGTTATGGATTTGACT+CGG 0.565798 1.2:+82454733 None:intergenic
GTCAAGATATTTGCATTGAG+CGG 0.567683 1.2:+82454464 None:intergenic
GCCACCGAGTCAAGCATACA+TGG 0.568072 1.2:-82454610 MS.gene065102:CDS
CACGTATTACTATGTGGGAC+TGG 0.571523 1.2:-82454100 MS.gene065102:CDS
TTGGACACGTATTACTATGT+GGG 0.577629 1.2:-82454105 MS.gene065102:CDS
GCAGAGCGATGTTTATAATG+TGG 0.578021 1.2:-82453965 MS.gene065102:CDS
GACAAAGGATTTGCCCGCGA+CGG 0.579763 1.2:-82453920 MS.gene065102:CDS
GACCAATCTCAGTGTCGCAA+TGG 0.580078 1.2:-82454444 MS.gene065102:CDS
GCTCGAAAATGGGATCGGCT+TGG 0.582434 1.2:+82454526 None:intergenic
ATCGGGAATATTCAACAGCA+AGG 0.582778 1.2:-82453709 MS.gene065102:CDS
GCTGGCTTAATCGGCTTAGG+CGG 0.584430 1.2:-82454276 MS.gene065102:CDS
ATTGGACACGTATTACTATG+TGG 0.586834 1.2:-82454106 MS.gene065102:CDS
TAGTAATACGTGTCCAATTG+CGG 0.587592 1.2:+82454111 None:intergenic
ATCCAACTAATGAATTTGCA+AGG 0.593800 1.2:+82453668 None:intergenic
ATTATCTCCGGAACAAGTCA+AGG 0.596309 1.2:-82454669 MS.gene065102:CDS
TGGTACCGGTTGATTCGGTA+GGG 0.596750 1.2:-82453777 MS.gene065102:CDS
CTGGTAGGGATAAGCGTCGG+CGG 0.597239 1.2:-82454081 MS.gene065102:CDS
TGTCTCTATCAAGTCTCCTA+CGG 0.597378 1.2:-82454417 MS.gene065102:CDS
TGCGTTTGTTAAGGGGACAA+AGG 0.599551 1.2:-82453935 MS.gene065102:CDS
AACGCCGATGGTAACTGTCT+CGG 0.600797 1.2:+82454362 None:intergenic
ATTACTATGTGGGACTGGTA+GGG 0.607196 1.2:-82454095 MS.gene065102:CDS
TCCGGAACAAGTCAAGGTAG+CGG 0.616809 1.2:-82454663 MS.gene065102:CDS
TGTGTATGAGCCATCACCGT+AGG 0.617012 1.2:+82454401 None:intergenic
ACCATGTATGCTTGACTCGG+TGG 0.621193 1.2:+82454609 None:intergenic
ATTTGACTCGGGCTGAGTCG+CGG 0.623914 1.2:+82454745 None:intergenic
GACAAGCGTGGAAGTTCCGA+CGG 0.625543 1.2:-82453854 MS.gene065102:CDS
AGGTTAAGAATGTAGCGCTA+GGG 0.626074 1.2:-82454335 MS.gene065102:CDS
AATTAATTAGCAACTGTTAG+GGG 0.626928 1.2:+82453642 None:intergenic
CGGGAATGGAGGGATTATTG+TGG 0.628617 1.2:-82454013 MS.gene065102:CDS
GGTACCAGGTAATTCTTCGC+CGG 0.631903 1.2:+82453793 None:intergenic
GTTCCGAGAATGTGCTAATG+GGG 0.633466 1.2:+82454992 None:intergenic
AGTTGGCTCGAAAATGGGAT+CGG 0.634624 1.2:+82454521 None:intergenic
CAACGACACGTCATCCGTCG+CGG 0.637283 1.2:+82453906 None:intergenic
CCGAAATGAAACGCCACCGT+CGG 0.637864 1.2:+82453838 None:intergenic
TCGGGAATATTCAACAGCAA+GGG 0.638017 1.2:-82453708 MS.gene065102:CDS
GTGTAGGAAGCTGATGCAGT+TGG 0.639824 1.2:+82454504 None:intergenic
AAACCCATAAGAAAATGAAG+AGG 0.641768 1.2:+82455015 None:intergenic
TTTGTCGTCGAAGACAAGCG+TGG 0.646119 1.2:-82453866 MS.gene065102:CDS
GATTATTGTGGATTCCGGCA+CGG 0.647183 1.2:-82454001 MS.gene065102:CDS
GCTTCCGGCGAAGAATTACC+TGG 0.648010 1.2:-82453797 MS.gene065102:CDS
AATTGCGGATTACGCCGTAA+CGG 0.648916 1.2:+82454126 None:intergenic
GGTGATGGCTCATACACAGT+AGG 0.649403 1.2:-82454396 MS.gene065102:CDS
AGAACCATGTATGCTTGACT+CGG 0.649421 1.2:+82454606 None:intergenic
AGCAGAAACATCAAGTAGTG+TGG 0.652436 1.2:+82454950 None:intergenic
GTTACCGAGACAGTTACCAT+CGG 0.672706 1.2:-82454366 MS.gene065102:CDS
TTGAAACCAAGTCAGAATCA+CGG 0.674620 1.2:+82454193 None:intergenic
TTCACAAGAGAGCTGCGTGT+AGG 0.678677 1.2:+82454488 None:intergenic
AAGCGTGGAAGTTCCGACGG+TGG 0.688631 1.2:-82453851 MS.gene065102:CDS
CGGATTACGCCGTAACGGCG+CGG 0.703290 1.2:+82454131 None:intergenic
GGACTGGTAGGGATAAGCGT+CGG 0.705431 1.2:-82454084 MS.gene065102:CDS
GGGTGTGGTCATAACAACGA+AGG 0.707295 1.2:-82454315 MS.gene065102:CDS
TATTGTGGATTCCGGCACGG+CGG 0.740065 1.2:-82453998 MS.gene065102:CDS
AATAATCCCTCCATTCCCGG+CGG 0.769319 1.2:+82454017 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTTGATATGTTTTTTGAA+TGG + chr1.2:82453766-82453785 None:intergenic 20.0%
!!! TTGATTTAACTTAGTTGTTA+TGG + chr1.2:82453970-82453989 None:intergenic 20.0%
AAACCCATAAGAAAATGAAG+AGG + chr1.2:82453677-82453696 None:intergenic 30.0%
ATCCAACTAATGAATTTGCA+AGG + chr1.2:82455024-82455043 None:intergenic 30.0%
TAGTTGTTATGGATTTGACT+CGG + chr1.2:82453959-82453978 None:intergenic 30.0%
! ATCACTTCTTTGTCGATTAT+CGG - chr1.2:82454962-82454981 MS.gene065102:CDS 30.0%
! CATCCTCTTCATTTTCTTAT+GGG - chr1.2:82453671-82453690 MS.gene065102:CDS 30.0%
! TCATCCTCTTCATTTTCTTA+TGG - chr1.2:82453670-82453689 MS.gene065102:CDS 30.0%
!! GTTGCTGTTGTTTAAAGTTT+TGG + chr1.2:82453800-82453819 None:intergenic 30.0%
AGTTGTTATGGATTTGACTC+GGG + chr1.2:82453958-82453977 None:intergenic 35.0%
ATTGGACACGTATTACTATG+TGG - chr1.2:82454583-82454602 MS.gene065102:CDS 35.0%
GAAAATGAAGAGGATGAAGT+TGG + chr1.2:82453667-82453686 None:intergenic 35.0%
GACCTTGCAAATTCATTAGT+TGG - chr1.2:82455019-82455038 MS.gene065102:CDS 35.0%
GTAATCAGCATGTGAAGAAA+GGG + chr1.2:82453898-82453917 None:intergenic 35.0%
GTCAAGATATTTGCATTGAG+CGG + chr1.2:82454228-82454247 None:intergenic 35.0%
TAGTAATACGTGTCCAATTG+CGG + chr1.2:82454581-82454600 None:intergenic 35.0%
TATTTCTCCAGAATCGGAAT+CGG - chr1.2:82454053-82454072 MS.gene065102:CDS 35.0%
TGTAATCAGCATGTGAAGAA+AGG + chr1.2:82453899-82453918 None:intergenic 35.0%
TTCAACACAGAGAAACTTTC+TGG - chr1.2:82453993-82454012 MS.gene065102:CDS 35.0%
TTGAAACCAAGTCAGAATCA+CGG + chr1.2:82454499-82454518 None:intergenic 35.0%
TTGGACACGTATTACTATGT+GGG - chr1.2:82454584-82454603 MS.gene065102:CDS 35.0%
! AACATCAAGTAGTGTGGTTT+TGG + chr1.2:82453736-82453755 None:intergenic 35.0%
! TCACTTCTTTGTCGATTATC+GGG - chr1.2:82454963-82454982 MS.gene065102:CDS 35.0%
AAGGTTAAGAATGTAGCGCT+AGG - chr1.2:82454353-82454372 MS.gene065102:CDS 40.0%
AGAACCATGTATGCTTGACT+CGG + chr1.2:82454086-82454105 None:intergenic 40.0%
AGCAGAAACATCAAGTAGTG+TGG + chr1.2:82453742-82453761 None:intergenic 40.0%
AGGTTAAGAATGTAGCGCTA+GGG - chr1.2:82454354-82454373 MS.gene065102:CDS 40.0%
AGTTACCATCGGCGTTAATA+AGG - chr1.2:82454334-82454353 MS.gene065102:CDS 40.0%
ATCGACAAAGAAGTGATCGT+TGG + chr1.2:82454959-82454978 None:intergenic 40.0%
ATCGGGAATATTCAACAGCA+AGG - chr1.2:82454980-82454999 MS.gene065102:CDS 40.0%
ATGTTCCGAGAATGTGCTAA+TGG + chr1.2:82453702-82453721 None:intergenic 40.0%
ATTATCTCCGGAACAAGTCA+AGG - chr1.2:82454020-82454039 MS.gene065102:CDS 40.0%
CTTAACCTTATTAACGCCGA+TGG + chr1.2:82454342-82454361 None:intergenic 40.0%
GCAGAGCGATGTTTATAATG+TGG - chr1.2:82454724-82454743 MS.gene065102:CDS 40.0%
TCGGGAATATTCAACAGCAA+GGG - chr1.2:82454981-82455000 MS.gene065102:CDS 40.0%
TGTCTCTATCAAGTCTCCTA+CGG - chr1.2:82454272-82454291 MS.gene065102:CDS 40.0%
TGTTCCGAGAATGTGCTAAT+GGG + chr1.2:82453701-82453720 None:intergenic 40.0%
TTTCCCCATTAGCACATTCT+CGG - chr1.2:82453694-82453713 MS.gene065102:CDS 40.0%
! ATTACTATGTGGGACTGGTA+GGG - chr1.2:82454594-82454613 MS.gene065102:CDS 40.0%
! GTTCGTGATGCGTTTGTTAA+GGG - chr1.2:82454746-82454765 MS.gene065102:CDS 40.0%
! TATTACTATGTGGGACTGGT+AGG - chr1.2:82454593-82454612 MS.gene065102:CDS 40.0%
! TTCGTGATGCGTTTGTTAAG+GGG - chr1.2:82454747-82454766 MS.gene065102:CDS 40.0%
! TTTCGGAGAAGGGAAAGTTT+TGG - chr1.2:82454868-82454887 MS.gene065102:CDS 40.0%
!! GTCGTTTTCGTATTGCTTAG+TGG - chr1.2:82454472-82454491 MS.gene065102:CDS 40.0%
!! TGACTTGTTCCGGAGATAAT+GGG + chr1.2:82454020-82454039 None:intergenic 40.0%
!! TTGACTTGTTCCGGAGATAA+TGG + chr1.2:82454021-82454040 None:intergenic 40.0%
!!! TAGTGTGGTTTTGGCATTGT+GGG + chr1.2:82453727-82453746 None:intergenic 40.0%
AAAGTCCCTACCGAATCAAC+CGG + chr1.2:82454920-82454939 None:intergenic 45.0%
CACAATAATCCCTCCATTCC+CGG + chr1.2:82454678-82454697 None:intergenic 45.0%
CACGTATTACTATGTGGGAC+TGG - chr1.2:82454589-82454608 MS.gene065102:CDS 45.0%
CTTTCTGGACCCATTATCTC+CGG - chr1.2:82454008-82454027 MS.gene065102:CDS 45.0%
GATTATTGTGGATTCCGGCA+CGG - chr1.2:82454688-82454707 MS.gene065102:CDS 45.0%
GGAGGGATTATTGTGGATTC+CGG - chr1.2:82454683-82454702 MS.gene065102:CDS 45.0%
GGCGAGTATTTCTCCAGAAT+CGG - chr1.2:82454047-82454066 MS.gene065102:CDS 45.0%
GTTACCGAGACAGTTACCAT+CGG - chr1.2:82454323-82454342 MS.gene065102:CDS 45.0%
GTTCCGAGAATGTGCTAATG+GGG + chr1.2:82453700-82453719 None:intergenic 45.0%
TACCATTGCGACACTGAGAT+TGG + chr1.2:82454250-82454269 None:intergenic 45.0%
TATCAAGTCTCCTACGGTGA+TGG - chr1.2:82454278-82454297 MS.gene065102:CDS 45.0%
TGGCGTTTCATTTCGGAGAA+GGG - chr1.2:82454858-82454877 MS.gene065102:CDS 45.0%
! AACAACGAAGGCTTGTTCGT+TGG - chr1.2:82454386-82454405 MS.gene065102:CDS 45.0%
! AATACCGGAGACGAGTTTTG+AGG - chr1.2:82454643-82454662 MS.gene065102:CDS 45.0%
! AATTGCGGATTACGCCGTAA+CGG + chr1.2:82454566-82454585 None:intergenic 45.0%
! GGTTCGTGATGCGTTTGTTA+AGG - chr1.2:82454745-82454764 MS.gene065102:CDS 45.0%
! TGCGTTTGTTAAGGGGACAA+AGG - chr1.2:82454754-82454773 MS.gene065102:CDS 45.0%
!! AGTTGGCTCGAAAATGGGAT+CGG + chr1.2:82454171-82454190 None:intergenic 45.0%
!! GATGCAGTTGGCTCGAAAAT+GGG + chr1.2:82454176-82454195 None:intergenic 45.0%
!! TGATGCAGTTGGCTCGAAAA+TGG + chr1.2:82454177-82454196 None:intergenic 45.0%
!! TTACCTGGTACCGGTTGATT+CGG - chr1.2:82454907-82454926 MS.gene065102:CDS 45.0%
!!! AGGGAAAGTTTTGGTGCTTC+CGG - chr1.2:82454877-82454896 MS.gene065102:CDS 45.0%
!!! AGTGTGGTTTTGGCATTGTG+GGG + chr1.2:82453726-82453745 None:intergenic 45.0%
!!! GTAGTGTGGTTTTGGCATTG+TGG + chr1.2:82453728-82453747 None:intergenic 45.0%
AACGCCGATGGTAACTGTCT+CGG + chr1.2:82454330-82454349 None:intergenic 50.0%
AAGAATGTAGCGCTAGGGTG+TGG - chr1.2:82454359-82454378 MS.gene065102:CDS 50.0%
AATAATCCCTCCATTCCCGG+CGG + chr1.2:82454675-82454694 None:intergenic 50.0%
ACACTGGAAGCGACATAAGC+TGG - chr1.2:82454108-82454127 MS.gene065102:CDS 50.0%
AGCTGTGCCGGAAAAGAAAG+TGG + chr1.2:82454443-82454462 None:intergenic 50.0%
AGTGGACCGTGATTCTGACT+TGG - chr1.2:82454490-82454509 MS.gene065102:CDS 50.0%
CACTGGAAGCGACATAAGCT+GGG - chr1.2:82454109-82454128 MS.gene065102:CDS 50.0%
CGGGAATGGAGGGATTATTG+TGG - chr1.2:82454676-82454695 MS.gene065102:CDS 50.0%
GACCAATCTCAGTGTCGCAA+TGG - chr1.2:82454245-82454264 MS.gene065102:CDS 50.0%
GCATACATGGTTCTCGACAC+TGG - chr1.2:82454092-82454111 MS.gene065102:CDS 50.0%
GGCGAAGAATTACCTGGTAC+CGG - chr1.2:82454898-82454917 MS.gene065102:CDS 50.0%
GGGTGTGGTCATAACAACGA+AGG - chr1.2:82454374-82454393 MS.gene065102:CDS 50.0%
GGTACCAGGTAATTCTTCGC+CGG + chr1.2:82454899-82454918 None:intergenic 50.0%
GTAACGGCGTCACGAGAAAA+CGG + chr1.2:82454539-82454558 None:intergenic 50.0%
GTGGCGTTTCATTTCGGAGA+AGG - chr1.2:82454857-82454876 MS.gene065102:CDS 50.0%
GTGTAGGAAGCTGATGCAGT+TGG + chr1.2:82454188-82454207 None:intergenic 50.0%
GTTACGGCGTAATCCGCAAT+TGG - chr1.2:82454565-82454584 MS.gene065102:CDS 50.0%
TCCACCTCAAAACTCGTCTC+CGG + chr1.2:82454650-82454669 None:intergenic 50.0%
TCCGGAACAAGTCAAGGTAG+CGG - chr1.2:82454026-82454045 MS.gene065102:CDS 50.0%
TGTGTATGAGCCATCACCGT+AGG + chr1.2:82454291-82454310 None:intergenic 50.0%
TTCACAAGAGAGCTGCGTGT+AGG + chr1.2:82454204-82454223 None:intergenic 50.0%
TTTGTCGTCGAAGACAAGCG+TGG - chr1.2:82454823-82454842 MS.gene065102:CDS 50.0%
! CGGTGGTCCACTTTCTTTTC+CGG - chr1.2:82454433-82454452 MS.gene065102:CDS 50.0%
! GGTGATGGCTCATACACAGT+AGG - chr1.2:82454293-82454312 MS.gene065102:CDS 50.0%
! GTTGGTGCAGCTGGCTTAAT+CGG - chr1.2:82454404-82454423 MS.gene065102:CDS 50.0%
!! ACCATGTATGCTTGACTCGG+TGG + chr1.2:82454083-82454102 None:intergenic 50.0%
!! TGGTACCGGTTGATTCGGTA+GGG - chr1.2:82454912-82454931 MS.gene065102:CDS 50.0%
!!! AGTTTTGAGGTGGATTCCGC+CGG - chr1.2:82454656-82454675 MS.gene065102:CDS 50.0%
CCGAAATGAAACGCCACCGT+CGG + chr1.2:82454854-82454873 None:intergenic 55.0%
CCGACGGTGGCGTTTCATTT+CGG - chr1.2:82454851-82454870 MS.gene065102:CDS 55.0%
CGGCGAGTTGCTTGCAATAC+CGG - chr1.2:82454628-82454647 MS.gene065102:CDS 55.0%
CTACCGAATCAACCGGTACC+AGG + chr1.2:82454913-82454932 None:intergenic 55.0%
GACAAAGGATTTGCCCGCGA+CGG - chr1.2:82454769-82454788 MS.gene065102:CDS 55.0%
GACAAGCGTGGAAGTTCCGA+CGG - chr1.2:82454835-82454854 MS.gene065102:CDS 55.0%
GACGTCGAATTGAGCTGTGC+CGG + chr1.2:82454455-82454474 None:intergenic 55.0%
GCCACCGAGTCAAGCATACA+TGG - chr1.2:82454079-82454098 MS.gene065102:CDS 55.0%
GCTCGAAAATGGGATCGGCT+TGG + chr1.2:82454166-82454185 None:intergenic 55.0%
GCTTCCGGCGAAGAATTACC+TGG - chr1.2:82454892-82454911 MS.gene065102:CDS 55.0%
TATTGTGGATTCCGGCACGG+CGG - chr1.2:82454691-82454710 MS.gene065102:CDS 55.0%
! GCAGCTGGCTTAATCGGCTT+AGG - chr1.2:82454410-82454429 MS.gene065102:CDS 55.0%
! GCTGGCTTAATCGGCTTAGG+CGG - chr1.2:82454413-82454432 MS.gene065102:CDS 55.0%
! GGACTGGTAGGGATAAGCGT+CGG - chr1.2:82454605-82454624 MS.gene065102:CDS 55.0%
!! ATCGGCTTGGCGGTAGCAAT+CGG + chr1.2:82454153-82454172 None:intergenic 55.0%
!! ATTTGACTCGGGCTGAGTCG+CGG + chr1.2:82453947-82453966 None:intergenic 55.0%
!! CGAAAATGGGATCGGCTTGG+CGG + chr1.2:82454163-82454182 None:intergenic 55.0%
!! CTGGTACCGGTTGATTCGGT+AGG - chr1.2:82454911-82454930 MS.gene065102:CDS 55.0%
!! GCCGCTACCTTGACTTGTTC+CGG + chr1.2:82454030-82454049 None:intergenic 55.0%
!! TGGCTCACCGATTCCGATTC+TGG + chr1.2:82454063-82454082 None:intergenic 55.0%
!!! ACCGGAGACGAGTTTTGAGG+TGG - chr1.2:82454646-82454665 MS.gene065102:CDS 55.0%
!!! GTTTTGAGGTGGATTCCGCC+GGG - chr1.2:82454657-82454676 MS.gene065102:CDS 55.0%
AACGACACGTCATCCGTCGC+GGG + chr1.2:82454785-82454804 None:intergenic 60.0%
AAGCGTGGAAGTTCCGACGG+TGG - chr1.2:82454838-82454857 MS.gene065102:CDS 60.0%
CAACGACACGTCATCCGTCG+CGG + chr1.2:82454786-82454805 None:intergenic 60.0%
TGGATTCCGCCGGGAATGGA+GGG - chr1.2:82454666-82454685 MS.gene065102:CDS 60.0%
! GGCTTAATCGGCTTAGGCGG+TGG - chr1.2:82454416-82454435 MS.gene065102:CDS 60.0%
! GGCTTGTTCGTTGGTGCAGC+TGG - chr1.2:82454395-82454414 MS.gene065102:CDS 60.0%
!! CTGGTAGGGATAAGCGTCGG+CGG - chr1.2:82454608-82454627 MS.gene065102:CDS 60.0%
ACGCCGTAACGGCGCGGTAA+CGG + chr1.2:82454555-82454574 None:intergenic 65.0%
ACGCCGTTACCGCGCCGTTA+CGG - chr1.2:82454549-82454568 MS.gene065102:CDS 65.0%
ATTCCGGCACGGCGGTGACA+CGG - chr1.2:82454699-82454718 MS.gene065102:CDS 65.0%
CGGATTACGCCGTAACGGCG+CGG + chr1.2:82454561-82454580 None:intergenic 65.0%
GTGGATTCCGCCGGGAATGG+AGG - chr1.2:82454665-82454684 MS.gene065102:CDS 65.0%
TGGCGGTAGCAATCGGCGCA+GGG + chr1.2:82454146-82454165 None:intergenic 65.0%
TTGGCGGTAGCAATCGGCGC+AGG + chr1.2:82454147-82454166 None:intergenic 65.0%
! GAGGTGGATTCCGCCGGGAA+TGG - chr1.2:82454662-82454681 MS.gene065102:CDS 65.0%
CAACCGTGTCACCGCCGTGC+CGG + chr1.2:82454705-82454724 None:intergenic 70.0%
GGCTGAGTCGCGGTCGAGTC+GGG + chr1.2:82453937-82453956 None:intergenic 70.0%
GGGCTGAGTCGCGGTCGAGT+CGG + chr1.2:82453938-82453957 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 82453648 82455063 82453648 ID=MS.gene065102
chr1.2 mRNA 82453648 82455063 82453648 ID=MS.gene065102.t1;Parent=MS.gene065102
chr1.2 exon 82453648 82455063 82453648 ID=MS.gene065102.t1.exon1;Parent=MS.gene065102.t1
chr1.2 CDS 82453648 82455063 82453648 ID=cds.MS.gene065102.t1;Parent=MS.gene065102.t1
Gene Sequence

>MS.gene065102

ATGGCTCATAAATTTTCCAACTTCATCCTCTTCATTTTCTTATGGGTTTCCCCATTAGCACATTCTCGGAACATACCCCACAATGCCAAAACCACACTACTTGATGTTTCTGCTTCCATTCAAAAAACATATCAAGTTCTCAACTTCAGCCAAAACTTTAAACAACAGCAACAACAACAACAACAACAAAAGAGTTCATTTACTTCATCAACATCAACACTCTCTTTGCAACTTCATTCACGTGCATCCCTTTCTTCACATGCTGATTACAAGTCACTAACATTATCCCGACTCGACCGCGACTCAGCCCGAGTCAAATCCATAACAACTAAGTTAAATCAACACTTCAACACAGAGAAACTTTCTGGACCCATTATCTCCGGAACAAGTCAAGGTAGCGGCGAGTATTTCTCCAGAATCGGAATCGGTGAGCCACCGAGTCAAGCATACATGGTTCTCGACACTGGAAGCGACATAAGCTGGGTACAATGCGCACCCTGCGCCGATTGCTACCGCCAAGCCGATCCCATTTTCGAGCCAACTGCATCAGCTTCCTACACGCAGCTCTCTTGTGAAGCCGCTCAATGCAAATATCTTGACCAATCTCAGTGTCGCAATGGTAACTGTCTCTATCAAGTCTCCTACGGTGATGGCTCATACACAGTAGGAGACTTTGTTACCGAGACAGTTACCATCGGCGTTAATAAGGTTAAGAATGTAGCGCTAGGGTGTGGTCATAACAACGAAGGCTTGTTCGTTGGTGCAGCTGGCTTAATCGGCTTAGGCGGTGGTCCACTTTCTTTTCCGGCACAGCTCAATTCGACGTCGTTTTCGTATTGCTTAGTGGACCGTGATTCTGACTTGGTTTCAACGTTAGAGTTTGATTCACCGTTTTCTCGTGACGCCGTTACCGCGCCGTTACGGCGTAATCCGCAATTGGACACGTATTACTATGTGGGACTGGTAGGGATAAGCGTCGGCGGCGAGTTGCTTGCAATACCGGAGACGAGTTTTGAGGTGGATTCCGCCGGGAATGGAGGGATTATTGTGGATTCCGGCACGGCGGTGACACGGTTGCAGAGCGATGTTTATAATGTGGTTCGTGATGCGTTTGTTAAGGGGACAAAGGATTTGCCCGCGACGGATGACGTGTCGTTGTTTGATACGTGTTATGATTTGTCGTCGAAGACAAGCGTGGAAGTTCCGACGGTGGCGTTTCATTTCGGAGAAGGGAAAGTTTTGGTGCTTCCGGCGAAGAATTACCTGGTACCGGTTGATTCGGTAGGGACTTTCTGTTTTGCGTTTGCTCCAACGATCACTTCTTTGTCGATTATCGGGAATATTCAACAGCAAGGGACACGTGTCAGTTTTGACCTTGCAAATTCATTAGTTGGATTTTCCCCTAACAGTTGCTAA
Protein sequence

>MS.gene065102.t1

MAHKFSNFILFIFLWVSPLAHSRNIPHNAKTTLLDVSASIQKTYQVLNFSQNFKQQQQQQQQQKSSFTSSTSTLSLQLHSRASLSSHADYKSLTLSRLDRDSARVKSITTKLNQHFNTEKLSGPIISGTSQGSGEYFSRIGIGEPPSQAYMVLDTGSDISWVQCAPCADCYRQADPIFEPTASASYTQLSCEAAQCKYLDQSQCRNGNCLYQVSYGDGSYTVGDFVTETVTIGVNKVKNVALGCGHNNEGLFVGAAGLIGLGGGPLSFPAQLNSTSFSYCLVDRDSDLVSTLEFDSPFSRDAVTAPLRRNPQLDTYYYVGLVGISVGGELLAIPETSFEVDSAGNGGIIVDSGTAVTRLQSDVYNVVRDAFVKGTKDLPATDDVSLFDTCYDLSSKTSVEVPTVAFHFGEGKVLVLPAKNYLVPVDSVGTFCFAFAPTITSLSIIGNIQQQGTRVSFDLANSLVGFSPNSC