AlfalfaGEDB | MS.gene065969

M. sativa cultivar XinJiangDaYe / MS.gene065969

KEGG: -
GO: GO:0000166 GO:0003674 GO:0005488 GO:0017076 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0036094 GO:0043167 GO:0043168 GO:0043531 GO:0097159 GO:0097367 GO:1901265 GO:1901363
PFAM: PF00931.25

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065969.t1	RHN42691.1	92.7	192	10	3	1	190	183	372	1.60E-88	335.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065969.t1	G7L7A4	92.7	192	10	3	1	190	65	254	1.2e-88	335.5

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050787	MS.gene065969	0.812425	4.27E-51	-1.69E-46
MS.gene051860	MS.gene065969	0.815453	9.17E-52	-1.69E-46
MS.gene054184	MS.gene065969	0.824955	6.10E-54	-1.69E-46
MS.gene054185	MS.gene065969	0.824253	8.92E-54	-1.69E-46
MS.gene054473	MS.gene065969	0.815906	7.27E-52	-1.69E-46
MS.gene054542	MS.gene065969	0.817402	3.36E-52	-1.69E-46
MS.gene055356	MS.gene065969	0.804609	1.99E-49	-1.69E-46
MS.gene055358	MS.gene065969	0.858228	9.51E-63	-1.69E-46

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PPI:

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065969.t1	MTR_8g087590	92.708	192	10	3	1	190	65	254	1.14e-123	349
MS.gene065969.t1	MTR_8g087650	79.781	183	35	1	13	193	13	195	1.33e-105	301
MS.gene065969.t1	MTR_8g087610	81.714	175	32	0	19	193	35	209	2.08e-104	299
MS.gene065969.t1	MTR_8g087640	80.447	179	32	1	11	189	58	233	2.25e-99	287
MS.gene065969.t1	MTR_8g087640	80.447	179	32	1	11	189	102	277	3.06e-99	288
MS.gene065969.t1	MTR_8g087630	78.889	180	36	1	14	193	14	191	1.08e-98	284
MS.gene065969.t1	MTR_8g087570	75.000	180	26	2	11	190	94	254	7.19e-86	253
MS.gene065969.t1	MTR_8g087570	75.000	180	26	2	11	190	56	216	8.68e-86	252
MS.gene065969.t1	MTR_8g087570	75.000	180	26	2	11	190	56	216	8.68e-86	252
MS.gene065969.t1	MTR_8g087600	65.789	152	23	1	25	176	7	129	4.77e-63	197

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 33 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATATAATTGGATTATGC+GGG	0.519176	4.3:+13363399	MS.gene065969:CDS
ACAAAGATTCAAACACAAAT+TGG	0.268354	4.3:+13363530	MS.gene065969:CDS
ACACAAATTGGTGATGCAAT+AGG	0.433183	4.3:+13363542	MS.gene065969:CDS
ACCATTATGATCTTCACCAA+AGG	0.590609	4.3:-13363750	None:intergenic
AGACTCGATTTAAGTGAGAT+TGG	0.459620	4.3:+13363722	MS.gene065969:CDS
AGAGATTGGTTCGGCAATCC+GGG	0.458252	4.3:+13363288	MS.gene065969:CDS
AGTGAGATTGGTATCCCCTT+TGG	0.416147	4.3:+13363734	MS.gene065969:CDS
ATGCAACAAGTTGATTCAGC+CGG	0.577268	4.3:-13363590	None:intergenic
ATTGCTCACATCTACAAGTT+TGG	0.339784	4.3:+13363781	MS.gene065969:CDS
CAATCCGGGAACAGTTTGGG+GGG	0.685818	4.3:+13363302	MS.gene065969:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GACCAAGGAGAAAATATAAT+TGG	+	chr4.3:13363389-13363408	MS.gene065969:CDS	30.0%
TTTATGAACCTTCATTTGCT+CGG	-	chr4.3:13363816-13363835	None:intergenic	30.0%
ACCATTATGATCTTCACCAA+AGG	-	chr4.3:13363753-13363772	None:intergenic	35.0%
AGACTCGATTTAAGTGAGAT+TGG	+	chr4.3:13363722-13363741	MS.gene065969:CDS	35.0%
ATTGCTCACATCTACAAGTT+TGG	+	chr4.3:13363781-13363800	MS.gene065969:CDS	35.0%
CATTATGATCTTCACCAAAG+GGG	-	chr4.3:13363751-13363770	None:intergenic	35.0%
CCATTATGATCTTCACCAAA+GGG	-	chr4.3:13363752-13363771	None:intergenic	35.0%
GAAGATCATACAGTACAACT+AGG	-	chr4.3:13363697-13363716	None:intergenic	35.0%
GTTGTACTGTATGATCTTCA+TGG	+	chr4.3:13363698-13363717	MS.gene065969:CDS	35.0%
CCCTTTGGTGAAGATCATAA+TGG	+	chr4.3:13363749-13363768	MS.gene065969:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	13363278	13363859	13363278	ID=MS.gene065969
chr4.3	mRNA	13363278	13363859	13363278	ID=MS.gene065969.t1;Parent=MS.gene065969
chr4.3	exon	13363278	13363859	13363278	ID=MS.gene065969.t1.exon1;Parent=MS.gene065969.t1
chr4.3	CDS	13363278	13363859	13363278	ID=cds.MS.gene065969.t1;Parent=MS.gene065969.t1

Gene Sequence

>MS.gene065969

ATGTCGTTTGAGAGATTGGTTCGGCAATCCGGGAACAGTTTGGGGGGCATTTATATTCCCAGCGTGGATGATAAGGTTAAAGAACAAATTATGAGTGCACTAAGAGTGAGAGACCAAGGAGAAAATATAATTGGATTATGCGGGCCAGAAAAAAGAGTCAAGCATTCTGCAAAAACAGCTATAAGAAGAGCTGAGAGAGATCAATTGTTCCAAAAGATTGTCACAGCAACTGTGACAAAGAAGCCAGATATTACAAAGATTCAAACACAAATTGGTGATGCAATAGGTCTGAATTTTGATGATAAAACGGATCCGGCTGAATCAACTTGTTGCATGTTTTTTGGTAATAGAAAAAGAATGGCAACTGCAGAAAGAGCTCATCTTTTGTGTGCTAAGATGAAAGGGCTGCAAACAGTCCTAGTTGTACTGTATGATCTTCATGGCAGACTCGATTTAAGTGAGATTGGTATCCCCTTTGGTGAAGATCATAATGGTTGCAAGATATTGCTCACATCTACAAGTTTGGAGGTTTTATCCGAGCAAATGAAGGTTCATAAATTGATACAATTGTCAGAGACATGA

Protein sequence

>MS.gene065969.t1

MSFERLVRQSGNSLGGIYIPSVDDKVKEQIMSALRVRDQGENIIGLCGPEKRVKHSAKTAIRRAERDQLFQKIVTATVTKKPDITKIQTQIGDAIGLNFDDKTDPAESTCCMFFGNRKRMATAERAHLLCAKMKGLQTVLVVLYDLHGRLDLSEIGIPFGEDHNGCKILLTSTSLEVLSEQMKVHKLIQLSET