AlfalfaGEDB | MS.gene065969

M. sativa cultivar XinJiangDaYe / MS.gene065969

KEGG: -
GO: GO:0000166 GO:0003674 GO:0005488 GO:0017076 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0036094 GO:0043167 GO:0043168 GO:0043531 GO:0097159 GO:0097367 GO:1901265 GO:1901363
PFAM: PF00931.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065969.t1	RHN42691.1	92.7	192	10	3	1	190	183	372	1.60E-88	335.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065969.t1	G7L7A4	92.7	192	10	3	1	190	65	254	1.2e-88	335.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050787	MS.gene065969	0.812425	4.27E-51	-1.69E-46
MS.gene051860	MS.gene065969	0.815453	9.17E-52	-1.69E-46
MS.gene054184	MS.gene065969	0.824955	6.10E-54	-1.69E-46
MS.gene054185	MS.gene065969	0.824253	8.92E-54	-1.69E-46
MS.gene054473	MS.gene065969	0.815906	7.27E-52	-1.69E-46
MS.gene054542	MS.gene065969	0.817402	3.36E-52	-1.69E-46
MS.gene055356	MS.gene065969	0.804609	1.99E-49	-1.69E-46
MS.gene055358	MS.gene065969	0.858228	9.51E-63	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065969.t1	MTR_8g087590	92.708	192	10	3	1	190	65	254	1.14e-123	349
MS.gene065969.t1	MTR_8g087650	79.781	183	35	1	13	193	13	195	1.33e-105	301
MS.gene065969.t1	MTR_8g087610	81.714	175	32	0	19	193	35	209	2.08e-104	299
MS.gene065969.t1	MTR_8g087640	80.447	179	32	1	11	189	58	233	2.25e-99	287
MS.gene065969.t1	MTR_8g087640	80.447	179	32	1	11	189	102	277	3.06e-99	288
MS.gene065969.t1	MTR_8g087630	78.889	180	36	1	14	193	14	191	1.08e-98	284
MS.gene065969.t1	MTR_8g087570	75.000	180	26	2	11	190	94	254	7.19e-86	253
MS.gene065969.t1	MTR_8g087570	75.000	180	26	2	11	190	56	216	8.68e-86	252
MS.gene065969.t1	MTR_8g087570	75.000	180	26	2	11	190	56	216	8.68e-86	252
MS.gene065969.t1	MTR_8g087600	65.789	152	23	1	25	176	7	129	4.77e-63	197
MS.gene065969.t1	MTR_8g087600	61.392	158	30	3	38	193	178	306	5.43e-54	174
MS.gene065969.t1	MTR_8g087555	66.892	148	21	1	46	193	4	123	1.15e-56	175
MS.gene065969.t1	MTR_3g013790	35.714	168	85	4	29	192	152	300	5.69e-22	93.2
MS.gene065969.t1	MTR_3g013790	35.714	168	85	4	29	192	152	300	7.92e-22	92.8
MS.gene065969.t1	MTR_2g072080	38.060	134	65	2	57	190	189	304	1.89e-21	91.7
MS.gene065969.t1	MTR_2g072820	35.821	134	68	1	57	190	187	302	7.27e-21	90.1
MS.gene065969.t1	MTR_2g072020	35.821	134	68	1	57	190	189	304	9.92e-21	89.7
MS.gene065969.t1	MTR_2g072150	37.313	134	66	3	57	190	189	304	1.58e-20	89.0
MS.gene065969.t1	MTR_2g072030	35.542	166	86	3	26	190	159	304	3.14e-20	88.2
MS.gene065969.t1	MTR_1g069615	37.903	124	59	2	63	186	79	184	5.13e-20	83.6
MS.gene065969.t1	MTR_1g036750	39.837	123	55	2	64	186	165	268	1.11e-19	86.7
MS.gene065969.t1	MTR_2g072110	35.821	134	68	1	57	190	189	304	1.49e-19	86.3
MS.gene065969.t1	MTR_2g072110	35.821	134	68	1	57	190	189	304	1.51e-19	86.3
MS.gene065969.t1	MTR_1g037010	39.837	123	55	2	64	186	197	300	2.47e-19	85.5
MS.gene065969.t1	MTR_2g071820	35.526	152	77	3	29	179	162	293	4.25e-19	84.7
MS.gene065969.t1	MTR_1g037150	37.805	164	76	6	26	186	147	287	8.27e-19	84.0
MS.gene065969.t1	MTR_2g071940	36.719	128	63	2	63	190	199	308	8.32e-19	84.0
MS.gene065969.t1	MTR_1g044020	36.196	163	78	4	27	186	161	300	1.09e-18	83.6
MS.gene065969.t1	MTR_2g071870	36.719	128	63	2	63	190	199	308	1.37e-18	83.6
MS.gene065969.t1	MTR_2g072090	35.938	128	64	2	63	190	199	308	2.31e-18	82.8
MS.gene065969.t1	MTR_0006s0240	37.805	164	76	6	26	186	160	300	2.75e-18	82.4
MS.gene065969.t1	MTR_1g041550	36.585	164	78	5	26	186	160	300	3.00e-18	82.4
MS.gene065969.t1	MTR_1g036860	35.795	176	84	7	19	186	145	299	3.22e-18	82.0
MS.gene065969.t1	MTR_2g071900	35.938	128	64	2	63	190	195	304	3.38e-18	82.0
MS.gene065969.t1	MTR_2g071890	35.526	152	77	3	29	179	162	293	3.79e-18	82.0
MS.gene065969.t1	MTR_0069s0040	34.146	164	82	4	26	186	159	299	7.22e-18	81.3
MS.gene065969.t1	MTR_0006s0080	39.024	123	56	2	64	186	197	300	9.66e-18	80.9
MS.gene065969.t1	MTR_1g051585	36.585	123	59	2	64	186	196	299	1.29e-17	80.5
MS.gene065969.t1	MTR_7g026250	32.386	176	94	6	18	190	119	272	1.43e-17	80.5
MS.gene065969.t1	MTR_0006s0230	39.837	123	55	3	64	186	197	300	1.58e-17	80.5
MS.gene065969.t1	MTR_1g037130	36.522	115	55	1	64	178	196	292	2.62e-17	79.7
MS.gene065969.t1	MTR_2g071980	35.938	128	64	2	63	190	195	304	5.21e-17	79.0
MS.gene065969.t1	MTR_2g071850	35.156	128	65	2	63	190	199	308	6.83e-17	78.6
MS.gene065969.t1	MTR_3g063010	31.818	176	95	6	18	190	144	297	2.20e-16	77.0
MS.gene065969.t1	MTR_6g463150	36.697	109	51	2	64	172	196	286	3.50e-16	76.3
MS.gene065969.t1	MTR_3g062420	31.902	163	87	5	18	177	144	285	4.69e-16	76.3
MS.gene065969.t1	MTR_8g059345	38.211	123	57	2	64	186	196	299	1.07e-15	75.1
MS.gene065969.t1	MTR_3g062220	31.818	176	95	6	18	190	144	297	1.31e-15	74.7
MS.gene065969.t1	MTR_3g062970	30.682	176	97	6	18	190	144	297	1.55e-15	74.3
MS.gene065969.t1	MTR_0006s0140	38.211	123	57	4	64	186	196	299	2.55e-15	73.2
MS.gene065969.t1	MTR_5g062770	29.487	156	90	3	36	190	147	283	5.15e-15	72.4
MS.gene065969.t1	MTR_3g467640	30.769	143	80	2	36	177	6	130	5.33e-15	72.8
MS.gene065969.t1	MTR_1g037250	33.918	171	82	7	24	186	158	305	9.02e-15	72.4
MS.gene065969.t1	MTR_0043s0110	30.286	175	99	5	18	190	143	296	2.83e-14	70.9
MS.gene065969.t1	MTR_1g036800	33.333	168	82	6	24	186	157	299	5.14e-14	70.1
MS.gene065969.t1	MTR_0068s0080	33.333	168	82	6	24	186	157	299	5.23e-14	70.1
MS.gene065969.t1	MTR_0068s0080	33.333	168	82	6	24	186	157	299	6.10e-14	70.1
MS.gene065969.t1	MTR_2g089975	31.481	162	89	4	26	186	159	299	6.86e-14	69.7
MS.gene065969.t1	MTR_0912s0010	29.609	179	95	6	18	190	143	296	8.19e-14	69.3
MS.gene065969.t1	MTR_0067s0070	28.358	134	77	2	57	190	183	297	1.98e-13	68.6
MS.gene065969.t1	MTR_3g451650	29.714	175	100	5	18	190	143	296	3.78e-13	67.4
MS.gene065969.t1	MTR_3g464840	29.762	168	87	5	28	190	156	297	6.43e-13	67.0
MS.gene065969.t1	MTR_3g062990	29.341	167	86	6	18	177	202	343	7.33e-13	66.6
MS.gene065969.t1	MTR_3g451150	27.841	176	102	5	18	190	144	297	9.08e-13	66.6
MS.gene065969.t1	MTR_3g452250	27.841	176	102	5	18	190	144	297	9.08e-13	66.6
MS.gene065969.t1	MTR_2g071910	48.387	62	32	0	125	186	11	72	1.68e-12	65.5
MS.gene065969.t1	MTR_2g071970	48.387	62	32	0	125	186	11	72	1.68e-12	65.5
MS.gene065969.t1	MTR_3g466580	28.571	168	90	5	28	190	156	298	4.24e-12	64.3
MS.gene065969.t1	MTR_3g048690	32.231	121	63	2	70	190	135	236	4.47e-12	64.3
MS.gene065969.t1	MTR_5g092410	29.936	157	78	6	24	174	148	278	4.55e-12	64.3
MS.gene065969.t1	MTR_3g466750	28.409	176	102	6	17	190	130	283	5.75e-12	64.3
MS.gene065969.t1	MTR_6g464050	37.500	96	42	2	83	178	832	909	2.42e-11	62.4
MS.gene065969.t1	MTR_3g464920	28.402	169	91	5	27	190	155	298	2.42e-11	62.4
MS.gene065969.t1	MTR_3g467340	27.273	143	85	2	36	177	161	285	5.50e-11	61.2
MS.gene065969.t1	MTR_3g062010	28.834	163	94	4	29	190	156	297	6.48e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 33 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGCAATCCGGGAACAGTTT+GGG	0.217269	4.3:+13363299	MS.gene065969:CDS
GACCAAGGAGAAAATATAAT+TGG	0.266280	4.3:+13363389	MS.gene065969:CDS
ACAAAGATTCAAACACAAAT+TGG	0.268354	4.3:+13363530	MS.gene065969:CDS
GTTTGAATCTTTGTAATATC+TGG	0.300279	4.3:-13363521	None:intergenic
CAGTTGCTGTGACAATCTTT+TGG	0.304172	4.3:-13363487	None:intergenic
TCGGCAATCCGGGAACAGTT+TGG	0.321768	4.3:+13363298	MS.gene065969:CDS
ATTGCTCACATCTACAAGTT+TGG	0.339784	4.3:+13363781	MS.gene065969:CDS
CATTATGTCGTTTGAGAGAT+TGG	0.354751	4.3:+13363274	None:intergenic
GTTGTACTGTATGATCTTCA+TGG	0.377791	4.3:+13363698	MS.gene065969:CDS
CCCTTTGGTGAAGATCATAA+TGG	0.415861	4.3:+13363749	MS.gene065969:CDS
AGTGAGATTGGTATCCCCTT+TGG	0.416147	4.3:+13363734	MS.gene065969:CDS
TATTCCCAGCGTGGATGATA+AGG	0.422594	4.3:+13363331	MS.gene065969:CDS
ACACAAATTGGTGATGCAAT+AGG	0.433183	4.3:+13363542	MS.gene065969:CDS
GAGAGATTGGTTCGGCAATC+CGG	0.440927	4.3:+13363287	MS.gene065969:CDS
GGCAATCCGGGAACAGTTTG+GGG	0.442842	4.3:+13363300	MS.gene065969:CDS
AGAGATTGGTTCGGCAATCC+GGG	0.458252	4.3:+13363288	MS.gene065969:CDS
AGACTCGATTTAAGTGAGAT+TGG	0.459620	4.3:+13363722	MS.gene065969:CDS
GCTCACATCTACAAGTTTGG+AGG	0.498220	4.3:+13363784	MS.gene065969:CDS
TTTATGAACCTTCATTTGCT+CGG	0.509437	4.3:-13363813	None:intergenic
AAAATATAATTGGATTATGC+GGG	0.519176	4.3:+13363399	MS.gene065969:CDS
CCATTATGATCTTCACCAAA+GGG	0.522614	4.3:-13363749	None:intergenic
GAAAATATAATTGGATTATG+CGG	0.532748	4.3:+13363398	MS.gene065969:CDS
TGTCGTTTGAGAGATTGGTT+CGG	0.553620	4.3:+13363279	MS.gene065969:CDS
ATGCAACAAGTTGATTCAGC+CGG	0.577268	4.3:-13363590	None:intergenic
TTAACCTTATCATCCACGCT+GGG	0.579493	4.3:-13363335	None:intergenic
GCAATCCGGGAACAGTTTGG+GGG	0.584978	4.3:+13363301	MS.gene065969:CDS
ACCATTATGATCTTCACCAA+AGG	0.590609	4.3:-13363750	None:intergenic
GCACTAAGAGTGAGAGACCA+AGG	0.594788	4.3:+13363374	MS.gene065969:CDS
TTTAACCTTATCATCCACGC+TGG	0.639042	4.3:-13363336	None:intergenic
GAAGATCATACAGTACAACT+AGG	0.653715	4.3:-13363694	None:intergenic
GGGCATTTATATTCCCAGCG+TGG	0.677027	4.3:+13363322	MS.gene065969:CDS
CAATCCGGGAACAGTTTGGG+GGG	0.685818	4.3:+13363302	MS.gene065969:CDS
CATTATGATCTTCACCAAAG+GGG	0.695532	4.3:-13363748	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATATAATTGGATTATGC+GGG	+	chr4.3:13363399-13363418	MS.gene065969:CDS	20.0%
!!	GAAAATATAATTGGATTATG+CGG	+	chr4.3:13363398-13363417	MS.gene065969:CDS	20.0%
!!!	TCTGAATTTTGATGATAAAA+CGG	+	chr4.3:13363565-13363584	MS.gene065969:CDS	20.0%
!!!	TTTTGGTAATAGAAAAAGAA+TGG	+	chr4.3:13363616-13363635	MS.gene065969:CDS	20.0%
!	ACAAAGATTCAAACACAAAT+TGG	+	chr4.3:13363530-13363549	MS.gene065969:CDS	25.0%
!	GTTTGAATCTTTGTAATATC+TGG	-	chr4.3:13363524-13363543	None:intergenic	25.0%
!!	ATCCAATTATATTTTCTCCT+TGG	-	chr4.3:13363394-13363413	None:intergenic	25.0%
	GACCAAGGAGAAAATATAAT+TGG	+	chr4.3:13363389-13363408	MS.gene065969:CDS	30.0%
	TTTATGAACCTTCATTTGCT+CGG	-	chr4.3:13363816-13363835	None:intergenic	30.0%
!	TTTTGTGTGCTAAGATGAAA+GGG	+	chr4.3:13363660-13363679	MS.gene065969:CDS	30.0%
!!	TTTTGATGATAAAACGGATC+CGG	+	chr4.3:13363571-13363590	MS.gene065969:CDS	30.0%
!!!	TCAACTTGTTGCATGTTTTT+TGG	+	chr4.3:13363599-13363618	MS.gene065969:CDS	30.0%
	ACCATTATGATCTTCACCAA+AGG	-	chr4.3:13363753-13363772	None:intergenic	35.0%
	AGACTCGATTTAAGTGAGAT+TGG	+	chr4.3:13363722-13363741	MS.gene065969:CDS	35.0%
	ATTGCTCACATCTACAAGTT+TGG	+	chr4.3:13363781-13363800	MS.gene065969:CDS	35.0%
	CATTATGATCTTCACCAAAG+GGG	-	chr4.3:13363751-13363770	None:intergenic	35.0%
	CCATTATGATCTTCACCAAA+GGG	-	chr4.3:13363752-13363771	None:intergenic	35.0%
	GAAGATCATACAGTACAACT+AGG	-	chr4.3:13363697-13363716	None:intergenic	35.0%
	GTTGTACTGTATGATCTTCA+TGG	+	chr4.3:13363698-13363717	MS.gene065969:CDS	35.0%
!	CTTTTGTGTGCTAAGATGAA+AGG	+	chr4.3:13363659-13363678	MS.gene065969:CDS	35.0%
!!	ACACAAATTGGTGATGCAAT+AGG	+	chr4.3:13363542-13363561	MS.gene065969:CDS	35.0%
!!!	GAATGCTTGACTCTTTTTTC+TGG	-	chr4.3:13363425-13363444	None:intergenic	35.0%
	CCCTTTGGTGAAGATCATAA+TGG	+	chr4.3:13363749-13363768	MS.gene065969:CDS	40.0%
	TTAACCTTATCATCCACGCT+GGG	-	chr4.3:13363338-13363357	None:intergenic	40.0%
	TTTAACCTTATCATCCACGC+TGG	-	chr4.3:13363339-13363358	None:intergenic	40.0%
!	CAGTTGCTGTGACAATCTTT+TGG	-	chr4.3:13363490-13363509	None:intergenic	40.0%
!	TGTCGTTTGAGAGATTGGTT+CGG	+	chr4.3:13363279-13363298	MS.gene065969:CDS	40.0%
!!	ATGCAACAAGTTGATTCAGC+CGG	-	chr4.3:13363593-13363612	None:intergenic	40.0%
!!	GGTTTTATCCGAGCAAATGA+AGG	+	chr4.3:13363805-13363824	MS.gene065969:CDS	40.0%
	AGTGAGATTGGTATCCCCTT+TGG	+	chr4.3:13363734-13363753	MS.gene065969:CDS	45.0%
	GCTCACATCTACAAGTTTGG+AGG	+	chr4.3:13363784-13363803	MS.gene065969:CDS	45.0%
	TATTCCCAGCGTGGATGATA+AGG	+	chr4.3:13363331-13363350	MS.gene065969:CDS	45.0%
	GCACTAAGAGTGAGAGACCA+AGG	+	chr4.3:13363374-13363393	MS.gene065969:CDS	50.0%
	GGGCATTTATATTCCCAGCG+TGG	+	chr4.3:13363322-13363341	MS.gene065969:CDS	50.0%
!!	AGAGATTGGTTCGGCAATCC+GGG	+	chr4.3:13363288-13363307	MS.gene065969:CDS	50.0%
!!	GAGAGATTGGTTCGGCAATC+CGG	+	chr4.3:13363287-13363306	MS.gene065969:CDS	50.0%
	AATGCCCCCCAAACTGTTCC+CGG	-	chr4.3:13363309-13363328	None:intergenic	55.0%
	CAATCCGGGAACAGTTTGGG+GGG	+	chr4.3:13363302-13363321	MS.gene065969:CDS	55.0%
	CGGCAATCCGGGAACAGTTT+GGG	+	chr4.3:13363299-13363318	MS.gene065969:CDS	55.0%
	GCAATCCGGGAACAGTTTGG+GGG	+	chr4.3:13363301-13363320	MS.gene065969:CDS	55.0%
	GGCAATCCGGGAACAGTTTG+GGG	+	chr4.3:13363300-13363319	MS.gene065969:CDS	55.0%
	TCGGCAATCCGGGAACAGTT+TGG	+	chr4.3:13363298-13363317	MS.gene065969:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	13363278	13363859	13363278	ID=MS.gene065969
chr4.3	mRNA	13363278	13363859	13363278	ID=MS.gene065969.t1;Parent=MS.gene065969
chr4.3	exon	13363278	13363859	13363278	ID=MS.gene065969.t1.exon1;Parent=MS.gene065969.t1
chr4.3	CDS	13363278	13363859	13363278	ID=cds.MS.gene065969.t1;Parent=MS.gene065969.t1

Gene Sequence

>MS.gene065969

ATGTCGTTTGAGAGATTGGTTCGGCAATCCGGGAACAGTTTGGGGGGCATTTATATTCCCAGCGTGGATGATAAGGTTAAAGAACAAATTATGAGTGCACTAAGAGTGAGAGACCAAGGAGAAAATATAATTGGATTATGCGGGCCAGAAAAAAGAGTCAAGCATTCTGCAAAAACAGCTATAAGAAGAGCTGAGAGAGATCAATTGTTCCAAAAGATTGTCACAGCAACTGTGACAAAGAAGCCAGATATTACAAAGATTCAAACACAAATTGGTGATGCAATAGGTCTGAATTTTGATGATAAAACGGATCCGGCTGAATCAACTTGTTGCATGTTTTTTGGTAATAGAAAAAGAATGGCAACTGCAGAAAGAGCTCATCTTTTGTGTGCTAAGATGAAAGGGCTGCAAACAGTCCTAGTTGTACTGTATGATCTTCATGGCAGACTCGATTTAAGTGAGATTGGTATCCCCTTTGGTGAAGATCATAATGGTTGCAAGATATTGCTCACATCTACAAGTTTGGAGGTTTTATCCGAGCAAATGAAGGTTCATAAATTGATACAATTGTCAGAGACATGA

Protein sequence

>MS.gene065969.t1

MSFERLVRQSGNSLGGIYIPSVDDKVKEQIMSALRVRDQGENIIGLCGPEKRVKHSAKTAIRRAERDQLFQKIVTATVTKKPDITKIQTQIGDAIGLNFDDKTDPAESTCCMFFGNRKRMATAERAHLLCAKMKGLQTVLVVLYDLHGRLDLSEIGIPFGEDHNGCKILLTSTSLEVLSEQMKVHKLIQLSET