Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066571.t1 | XP_003626280.1 | 94.3 | 193 | 11 | 0 | 1 | 193 | 218 | 410 | 7.90E-91 | 343.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066571.t1 | Q9FZ33 | 57.3 | 192 | 78 | 1 | 2 | 193 | 213 | 400 | 1.0e-54 | 214.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066571.t1 | G7KYN0 | 94.3 | 193 | 11 | 0 | 1 | 193 | 218 | 410 | 5.7e-91 | 343.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049190 | MS.gene066571 | 0.803649 | 3.15E-49 | -1.69E-46 |
| MS.gene049418 | MS.gene066571 | 0.82365 | 1.24E-53 | -1.69E-46 |
| MS.gene049887 | MS.gene066571 | 0.814249 | 1.70E-51 | -1.69E-46 |
| MS.gene050630 | MS.gene066571 | 0.82155 | 3.80E-53 | -1.69E-46 |
| MS.gene050807 | MS.gene066571 | 0.808995 | 2.35E-50 | -1.69E-46 |
| MS.gene05157 | MS.gene066571 | 0.808316 | 3.29E-50 | -1.69E-46 |
| MS.gene051720 | MS.gene066571 | 0.811474 | 6.88E-51 | -1.69E-46 |
| MS.gene052665 | MS.gene066571 | 0.829366 | 5.36E-55 | -1.69E-46 |
| MS.gene052732 | MS.gene066571 | 0.839258 | 1.76E-57 | -1.69E-46 |
| MS.gene053144 | MS.gene066571 | 0.852729 | 3.81E-61 | -1.69E-46 |
| MS.gene053174 | MS.gene066571 | 0.820853 | 5.51E-53 | -1.69E-46 |
| MS.gene053581 | MS.gene066571 | 0.802895 | 4.51E-49 | -1.69E-46 |
| MS.gene053599 | MS.gene066571 | 0.811404 | 7.12E-51 | -1.69E-46 |
| MS.gene054550 | MS.gene066571 | 0.832285 | 1.03E-55 | -1.69E-46 |
| MS.gene054584 | MS.gene066571 | 0.870223 | 1.72E-66 | -1.69E-46 |
| MS.gene055465 | MS.gene066571 | 0.810935 | 9.00E-51 | -1.69E-46 |
| MS.gene055468 | MS.gene066571 | 0.805947 | 1.04E-49 | -1.69E-46 |
| MS.gene055469 | MS.gene066571 | 0.804674 | 1.93E-49 | -1.69E-46 |
| MS.gene055537 | MS.gene066571 | 0.834896 | 2.30E-56 | -1.69E-46 |
| MS.gene055580 | MS.gene066571 | -0.846616 | 1.94E-59 | -1.69E-46 |
| MS.gene055891 | MS.gene066571 | 0.833257 | 5.92E-56 | -1.69E-46 |
| MS.gene056043 | MS.gene066571 | -0.835968 | 1.23E-56 | -1.69E-46 |
| MS.gene056797 | MS.gene066571 | 0.803465 | 3.44E-49 | -1.69E-46 |
| MS.gene060194 | MS.gene066571 | 0.805984 | 1.03E-49 | -1.69E-46 |
| MS.gene060587 | MS.gene066571 | 0.834106 | 3.63E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066571.t1 | MTR_7g113420 | 94.819 | 193 | 10 | 0 | 1 | 193 | 218 | 410 | 5.92e-120 | 346 |
| MS.gene066571.t1 | MTR_7g113420 | 94.819 | 193 | 10 | 0 | 1 | 193 | 218 | 410 | 1.45e-118 | 345 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066571.t1 | AT1G54990 | 60.938 | 192 | 71 | 1 | 2 | 193 | 213 | 400 | 3.40e-75 | 233 |
Find 55 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAAAGTTTGTTACTCATTC+TGG | 0.188700 | 7.4:+3268766 | MS.gene066571:CDS |
| GGTTTCTGAGAATTTAAATT+TGG | 0.212168 | 7.4:+3268369 | MS.gene066571:CDS |
| GTGAATAAACCTATTGATTT+AGG | 0.213075 | 7.4:+3268241 | MS.gene066571:CDS |
| TGAAGAGATTGGAAAAGTTT+TGG | 0.215909 | 7.4:+3268267 | MS.gene066571:CDS |
| CTTTAGGGTTTACTGCAAAT+TGG | 0.234894 | 7.4:+3268347 | MS.gene066571:CDS |
| GCCCGTGCCACCTTCAAATT+CGG | 0.300130 | 7.4:+3268417 | MS.gene066571:CDS |
| AGGATTAGCTCCTGTTCATT+TGG | 0.321069 | 7.4:+3268309 | MS.gene066571:CDS |
| GGGTTGCTGGTGCTCTTCCT+TGG | 0.322738 | 7.4:+3268743 | MS.gene066571:CDS |
| GGAGAGGTTATTGCTACATT+AGG | 0.331260 | 7.4:+3268289 | MS.gene066571:CDS |
| CGCGAAGGGAATCGGGTTGC+TGG | 0.337738 | 7.4:+3268730 | MS.gene066571:CDS |
| GGAACATCCAAAACCCAAAT+AGG | 0.346484 | 7.4:-3268448 | None:intergenic |
| CATAGGGTGCTGTTGAAGTC+TGG | 0.362685 | 7.4:+3268568 | MS.gene066571:CDS |
| TCTTCAGGACCTAAATCAAT+AGG | 0.364136 | 7.4:-3268250 | None:intergenic |
| GGTTGCTGGTGCTCTTCCTT+GGG | 0.365766 | 7.4:+3268744 | MS.gene066571:CDS |
| AATGGAGTCGCGAAGGGAAT+CGG | 0.375141 | 7.4:+3268722 | MS.gene066571:CDS |
| TAGCTTTGATTTAGCTGAAT+GGG | 0.376044 | 7.4:+3268636 | MS.gene066571:CDS |
| TACTCATTCTGGAGGCCGTT+GGG | 0.379063 | 7.4:+3268777 | MS.gene066571:CDS |
| TTTAGGGTTTACTGCAAATT+GGG | 0.387005 | 7.4:+3268348 | MS.gene066571:CDS |
| AGGAAAGCGGTTGTGGCTAT+TGG | 0.398736 | 7.4:+3268598 | MS.gene066571:CDS |
| TTCTGGAGGCCGTTGGGCTC+AGG | 0.412514 | 7.4:+3268783 | MS.gene066571:CDS |
| TTCTAAGAGAATTGGTGGTT+TGG | 0.414206 | 7.4:+3268534 | MS.gene066571:CDS |
| CCCGTGCCACCTTCAAATTC+GGG | 0.415386 | 7.4:+3268418 | MS.gene066571:CDS |
| ATGGAGTCGCGAAGGGAATC+GGG | 0.426920 | 7.4:+3268723 | MS.gene066571:CDS |
| GATTTAGGTCCTGAAGAGAT+TGG | 0.432271 | 7.4:+3268256 | MS.gene066571:CDS |
| TACTGTTCTAAGAGAATTGG+TGG | 0.443206 | 7.4:+3268529 | MS.gene066571:CDS |
| TTACTCATTCTGGAGGCCGT+TGG | 0.460405 | 7.4:+3268776 | MS.gene066571:CDS |
| TCATGCAAAACCAAATGAAC+AGG | 0.463756 | 7.4:-3268319 | None:intergenic |
| ATAGGGTGCTGTTGAAGTCT+GGG | 0.467959 | 7.4:+3268569 | MS.gene066571:CDS |
| CTAGCTTTGATTTAGCTGAA+TGG | 0.469276 | 7.4:+3268635 | MS.gene066571:CDS |
| GCTGAATGGGGTGGTTCTGA+TGG | 0.473143 | 7.4:+3268649 | MS.gene066571:CDS |
| GGATGGAAGGAAAGCGGTTG+TGG | 0.475697 | 7.4:+3268591 | MS.gene066571:CDS |
| ATATGCCAATGCAACTGATC+TGG | 0.483350 | 7.4:+3268680 | MS.gene066571:CDS |
| CCCGAATTTGAAGGTGGCAC+GGG | 0.505835 | 7.4:-3268418 | None:intergenic |
| GGAAAAGCACCCGAATTTGA+AGG | 0.517603 | 7.4:-3268427 | None:intergenic |
| AACTGATCTGGTCTAGTGAT+TGG | 0.528070 | 7.4:+3268692 | MS.gene066571:CDS |
| CTAGTGATTGGTCTGTAGAA+TGG | 0.529699 | 7.4:+3268704 | MS.gene066571:CDS |
| TTTGGATGCTGATGCACATA+GGG | 0.533671 | 7.4:+3268552 | MS.gene066571:CDS |
| AGCTTTGATTTAGCTGAATG+GGG | 0.545831 | 7.4:+3268637 | MS.gene066571:CDS |
| CTAGACCAGATCAGTTGCAT+TGG | 0.554156 | 7.4:-3268685 | None:intergenic |
| GTTTGGATGCTGATGCACAT+AGG | 0.560246 | 7.4:+3268551 | MS.gene066571:CDS |
| ACCCGAATTTGAAGGTGGCA+CGG | 0.569982 | 7.4:-3268419 | None:intergenic |
| AAAACTACCTCTCTAATCAA+AGG | 0.579564 | 7.4:-3268469 | None:intergenic |
| GGATGTTCCTTTGATTAGAG+AGG | 0.589634 | 7.4:+3268462 | MS.gene066571:CDS |
| CTGTAGAATGGAGTCGCGAA+GGG | 0.589757 | 7.4:+3268716 | MS.gene066571:CDS |
| GTGCTGTTGAAGTCTGGGGA+TGG | 0.594356 | 7.4:+3268574 | MS.gene066571:CDS |
| TTTGATTTAGCTGAATGGGG+TGG | 0.595613 | 7.4:+3268640 | MS.gene066571:CDS |
| AAGTTTGTTACTCATTCTGG+AGG | 0.604446 | 7.4:+3268769 | MS.gene066571:CDS |
| TTCTAGCTAGAATGTCAAAG+AGG | 0.614198 | 7.4:+3268215 | None:intergenic |
| TCTGTAGAATGGAGTCGCGA+AGG | 0.618026 | 7.4:+3268715 | MS.gene066571:CDS |
| TGTTGAAGTCTGGGGATGGA+AGG | 0.618688 | 7.4:+3268578 | MS.gene066571:CDS |
| GTCTGGGGATGGAAGGAAAG+CGG | 0.630612 | 7.4:+3268585 | MS.gene066571:CDS |
| TGAGTAACAAACTTTGCCCA+AGG | 0.635006 | 7.4:-3268760 | None:intergenic |
| TTGTGCACACCTGAGCCCAA+CGG | 0.637219 | 7.4:-3268792 | None:intergenic |
| AAAGCACCCGAATTTGAAGG+TGG | 0.688232 | 7.4:-3268424 | None:intergenic |
| TAGGGTGCTGTTGAAGTCTG+GGG | 0.693836 | 7.4:+3268570 | MS.gene066571:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GGTTTCTGAGAATTTAAATT+TGG | + | chr7.4:3268369-3268388 | MS.gene066571:CDS | 25.0% |
| !!! | GTGAATAAACCTATTGATTT+AGG | + | chr7.4:3268241-3268260 | MS.gene066571:CDS | 25.0% |
| !!! | TTTTACTGTTCTAAGAGAAT+TGG | + | chr7.4:3268526-3268545 | MS.gene066571:CDS | 25.0% |
| AAAACTACCTCTCTAATCAA+AGG | - | chr7.4:3268472-3268491 | None:intergenic | 30.0% | |
| ! | AAAACTTTTCCAATCTCTTC+AGG | - | chr7.4:3268268-3268287 | None:intergenic | 30.0% |
| ! | GAAGAGATTGGAAAAGTTTT+GGG | + | chr7.4:3268268-3268287 | MS.gene066571:CDS | 30.0% |
| ! | TGAAGAGATTGGAAAAGTTT+TGG | + | chr7.4:3268267-3268286 | MS.gene066571:CDS | 30.0% |
| ! | TTGATTAGAGAGGTAGTTTT+AGG | + | chr7.4:3268472-3268491 | MS.gene066571:CDS | 30.0% |
| ! | TTTAGGGTTTACTGCAAATT+GGG | + | chr7.4:3268348-3268367 | MS.gene066571:CDS | 30.0% |
| ! | TTTTGCATGATTCAGCTTTA+GGG | + | chr7.4:3268332-3268351 | MS.gene066571:CDS | 30.0% |
| !! | ACCACTTTTGCAAAAACATA+AGG | - | chr7.4:3268502-3268521 | None:intergenic | 30.0% |
| !! | TAGCTTTGATTTAGCTGAAT+GGG | + | chr7.4:3268636-3268655 | MS.gene066571:CDS | 30.0% |
| !! | TCCTTATGTTTTTGCAAAAG+TGG | + | chr7.4:3268498-3268517 | MS.gene066571:CDS | 30.0% |
| !! | TGATTAGAGAGGTAGTTTTA+GGG | + | chr7.4:3268473-3268492 | MS.gene066571:CDS | 30.0% |
| TCATGCAAAACCAAATGAAC+AGG | - | chr7.4:3268322-3268341 | None:intergenic | 35.0% | |
| TCTTCAGGACCTAAATCAAT+AGG | - | chr7.4:3268253-3268272 | None:intergenic | 35.0% | |
| ! | AGCTTTGATTTAGCTGAATG+GGG | + | chr7.4:3268637-3268656 | MS.gene066571:CDS | 35.0% |
| ! | GTTTTGCATGATTCAGCTTT+AGG | + | chr7.4:3268331-3268350 | MS.gene066571:CDS | 35.0% |
| ! | TTCTAAGAGAATTGGTGGTT+TGG | + | chr7.4:3268534-3268553 | MS.gene066571:CDS | 35.0% |
| !! | AAGTTTGTTACTCATTCTGG+AGG | + | chr7.4:3268769-3268788 | MS.gene066571:CDS | 35.0% |
| !! | CTAGCTTTGATTTAGCTGAA+TGG | + | chr7.4:3268635-3268654 | MS.gene066571:CDS | 35.0% |
| !! | CTTTAGGGTTTACTGCAAAT+TGG | + | chr7.4:3268347-3268366 | MS.gene066571:CDS | 35.0% |
| !! | GCAAAGTTTGTTACTCATTC+TGG | + | chr7.4:3268766-3268785 | MS.gene066571:CDS | 35.0% |
| !! | TACTGTTCTAAGAGAATTGG+TGG | + | chr7.4:3268529-3268548 | MS.gene066571:CDS | 35.0% |
| !! | TGCTTTTCCTATTTGGGTTT+TGG | + | chr7.4:3268441-3268460 | MS.gene066571:CDS | 35.0% |
| AACTGATCTGGTCTAGTGAT+TGG | + | chr7.4:3268692-3268711 | MS.gene066571:CDS | 40.0% | |
| ATATGCCAATGCAACTGATC+TGG | + | chr7.4:3268680-3268699 | MS.gene066571:CDS | 40.0% | |
| GATTTAGGTCCTGAAGAGAT+TGG | + | chr7.4:3268256-3268275 | MS.gene066571:CDS | 40.0% | |
| GGAACATCCAAAACCCAAAT+AGG | - | chr7.4:3268451-3268470 | None:intergenic | 40.0% | |
| GGAGAGGTTATTGCTACATT+AGG | + | chr7.4:3268289-3268308 | MS.gene066571:CDS | 40.0% | |
| GGATGTTCCTTTGATTAGAG+AGG | + | chr7.4:3268462-3268481 | MS.gene066571:CDS | 40.0% | |
| TGAGTAACAAACTTTGCCCA+AGG | - | chr7.4:3268763-3268782 | None:intergenic | 40.0% | |
| TTTGGATGCTGATGCACATA+GGG | + | chr7.4:3268552-3268571 | MS.gene066571:CDS | 40.0% | |
| ! | ATTCGGGTGCTTTTCCTATT+TGG | + | chr7.4:3268434-3268453 | MS.gene066571:CDS | 40.0% |
| ! | TTCGGGTGCTTTTCCTATTT+GGG | + | chr7.4:3268435-3268454 | MS.gene066571:CDS | 40.0% |
| ! | TTTGATTTAGCTGAATGGGG+TGG | + | chr7.4:3268640-3268659 | MS.gene066571:CDS | 40.0% |
| !! | AGGATTAGCTCCTGTTCATT+TGG | + | chr7.4:3268309-3268328 | MS.gene066571:CDS | 40.0% |
| !! | CTAGTGATTGGTCTGTAGAA+TGG | + | chr7.4:3268704-3268723 | MS.gene066571:CDS | 40.0% |
| !!! | GATTGGAAAAGTTTTGGGAG+AGG | + | chr7.4:3268273-3268292 | MS.gene066571:CDS | 40.0% |
| CTAGACCAGATCAGTTGCAT+TGG | - | chr7.4:3268688-3268707 | None:intergenic | 45.0% | |
| GTTTGGATGCTGATGCACAT+AGG | + | chr7.4:3268551-3268570 | MS.gene066571:CDS | 45.0% | |
| ! | AAAGCACCCGAATTTGAAGG+TGG | - | chr7.4:3268427-3268446 | None:intergenic | 45.0% |
| !! | ATAGGGTGCTGTTGAAGTCT+GGG | + | chr7.4:3268569-3268588 | MS.gene066571:CDS | 45.0% |
| !! | GGAAAAGCACCCGAATTTGA+AGG | - | chr7.4:3268430-3268449 | None:intergenic | 45.0% |
| AATGGAGTCGCGAAGGGAAT+CGG | + | chr7.4:3268722-3268741 | MS.gene066571:CDS | 50.0% | |
| ACCCGAATTTGAAGGTGGCA+CGG | - | chr7.4:3268422-3268441 | None:intergenic | 50.0% | |
| AGGAAAGCGGTTGTGGCTAT+TGG | + | chr7.4:3268598-3268617 | MS.gene066571:CDS | 50.0% | |
| CTGTAGAATGGAGTCGCGAA+GGG | + | chr7.4:3268716-3268735 | MS.gene066571:CDS | 50.0% | |
| TCTGTAGAATGGAGTCGCGA+AGG | + | chr7.4:3268715-3268734 | MS.gene066571:CDS | 50.0% | |
| TGTTGAAGTCTGGGGATGGA+AGG | + | chr7.4:3268578-3268597 | MS.gene066571:CDS | 50.0% | |
| ! | TACTCATTCTGGAGGCCGTT+GGG | + | chr7.4:3268777-3268796 | MS.gene066571:CDS | 50.0% |
| ! | TTACTCATTCTGGAGGCCGT+TGG | + | chr7.4:3268776-3268795 | MS.gene066571:CDS | 50.0% |
| !! | CATAGGGTGCTGTTGAAGTC+TGG | + | chr7.4:3268568-3268587 | MS.gene066571:CDS | 50.0% |
| !! | TAGGGTGCTGTTGAAGTCTG+GGG | + | chr7.4:3268570-3268589 | MS.gene066571:CDS | 50.0% |
| ATGGAGTCGCGAAGGGAATC+GGG | + | chr7.4:3268723-3268742 | MS.gene066571:CDS | 55.0% | |
| CCCGAATTTGAAGGTGGCAC+GGG | - | chr7.4:3268421-3268440 | None:intergenic | 55.0% | |
| CCCGTGCCACCTTCAAATTC+GGG | + | chr7.4:3268418-3268437 | MS.gene066571:CDS | 55.0% | |
| GCCCGTGCCACCTTCAAATT+CGG | + | chr7.4:3268417-3268436 | MS.gene066571:CDS | 55.0% | |
| GGATGGAAGGAAAGCGGTTG+TGG | + | chr7.4:3268591-3268610 | MS.gene066571:CDS | 55.0% | |
| GGTTGCTGGTGCTCTTCCTT+GGG | + | chr7.4:3268744-3268763 | MS.gene066571:CDS | 55.0% | |
| GTCTGGGGATGGAAGGAAAG+CGG | + | chr7.4:3268585-3268604 | MS.gene066571:CDS | 55.0% | |
| ! | GTGCTGTTGAAGTCTGGGGA+TGG | + | chr7.4:3268574-3268593 | MS.gene066571:CDS | 55.0% |
| !! | GCTGAATGGGGTGGTTCTGA+TGG | + | chr7.4:3268649-3268668 | MS.gene066571:CDS | 55.0% |
| GGGTTGCTGGTGCTCTTCCT+TGG | + | chr7.4:3268743-3268762 | MS.gene066571:CDS | 60.0% | |
| ! | CGCGAAGGGAATCGGGTTGC+TGG | + | chr7.4:3268730-3268749 | MS.gene066571:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 3268226 | 3268804 | 3268226 | ID=MS.gene066571 |
| chr7.4 | mRNA | 3268226 | 3268804 | 3268226 | ID=MS.gene066571.t1;Parent=MS.gene066571 |
| chr7.4 | exon | 3268226 | 3268804 | 3268226 | ID=MS.gene066571.t1.exon1;Parent=MS.gene066571.t1 |
| chr7.4 | CDS | 3268226 | 3268804 | 3268226 | ID=cds.MS.gene066571.t1;Parent=MS.gene066571.t1 |
| Gene Sequence |
| Protein sequence |
