Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066574.t1 | XP_003613998.1 | 97.3 | 557 | 15 | 0 | 292 | 848 | 4 | 560 | 0.00E+00 | 1095.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066574.t1 | A7T167 | 37.7 | 491 | 269 | 9 | 348 | 831 | 27 | 487 | 9.2e-84 | 313.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066574.t1 | G7JXV5 | 97.3 | 557 | 15 | 0 | 292 | 848 | 4 | 560 | 0.0e+00 | 1095.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049427 | MS.gene066574 | 0.818471 | 1.93E-52 | -1.69E-46 |
| MS.gene049805 | MS.gene066574 | 0.81193 | 5.47E-51 | -1.69E-46 |
| MS.gene052421 | MS.gene066574 | 0.811852 | 5.69E-51 | -1.69E-46 |
| MS.gene055412 | MS.gene066574 | 0.821336 | 4.26E-53 | -1.69E-46 |
| MS.gene057011 | MS.gene066574 | 0.815946 | 7.12E-52 | -1.69E-46 |
| MS.gene058019 | MS.gene066574 | 0.815293 | 9.96E-52 | -1.69E-46 |
| MS.gene059455 | MS.gene066574 | 0.806586 | 7.65E-50 | -1.69E-46 |
| MS.gene059960 | MS.gene066574 | 0.819147 | 1.35E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066574.t1 | MTR_5g043560 | 98.743 | 557 | 7 | 0 | 292 | 848 | 4 | 560 | 0.0 | 1143 |
| MS.gene066574.t1 | MTR_5g043560 | 98.321 | 536 | 9 | 0 | 292 | 827 | 4 | 539 | 0.0 | 1093 |
| MS.gene066574.t1 | MTR_4g082640 | 83.005 | 559 | 89 | 3 | 291 | 848 | 3 | 556 | 0.0 | 960 |
| MS.gene066574.t1 | MTR_5g043580 | 97.110 | 173 | 5 | 0 | 1 | 173 | 1 | 173 | 2.70e-116 | 350 |
| MS.gene066574.t1 | MTR_6g025065 | 72.840 | 81 | 20 | 1 | 557 | 637 | 76 | 154 | 1.15e-30 | 118 |
| MS.gene066574.t1 | MTR_4g008800 | 56.566 | 99 | 41 | 1 | 555 | 651 | 69 | 167 | 6.82e-25 | 103 |
| MS.gene066574.t1 | MTR_1g007640 | 35.465 | 172 | 96 | 6 | 354 | 514 | 42 | 209 | 6.52e-19 | 87.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066574.t1 | AT1G69340 | 78.919 | 555 | 115 | 1 | 296 | 848 | 8 | 562 | 0.0 | 919 |
| MS.gene066574.t1 | AT1G69340 | 78.919 | 555 | 115 | 1 | 296 | 848 | 8 | 562 | 0.0 | 919 |
| MS.gene066574.t1 | AT2G03420 | 74.038 | 104 | 27 | 0 | 69 | 172 | 63 | 166 | 2.24e-47 | 166 |
| MS.gene066574.t1 | AT2G40600 | 32.667 | 150 | 88 | 5 | 370 | 508 | 79 | 226 | 6.62e-15 | 76.3 |
Find 203 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAATTTCAGATAGATTTA+AGG | 0.122409 | 5.1:+32610694 | None:intergenic |
| CTTGAGCTTCTAATTGAAAA+TGG | 0.164198 | 5.1:-32613050 | MS.gene066574:CDS |
| GTATAAGGCTTCTGTATTAT+TGG | 0.242635 | 5.1:+32609585 | None:intergenic |
| CTTATTATTGAGTTTGATTA+TGG | 0.243105 | 5.1:-32618865 | MS.gene066574:CDS |
| ATCATATTTAGATTCATTTC+TGG | 0.247633 | 5.1:-32610903 | MS.gene066574:CDS |
| TTGGCGTTCCAATCGGGAAC+TGG | 0.301774 | 5.1:-32618311 | MS.gene066574:CDS |
| AAGTGATGAAACTAGTTTCT+TGG | 0.301809 | 5.1:+32620631 | None:intergenic |
| TTTGCTAATTTGCTTGGATT+TGG | 0.302487 | 5.1:-32610791 | MS.gene066574:CDS |
| TACATCCTTCACCCAACTTT+AGG | 0.314369 | 5.1:-32608216 | MS.gene066574:CDS |
| CTTCAATTGCAATTCTTCTC+TGG | 0.318920 | 5.1:-32623185 | MS.gene066574:CDS |
| CTGCTTACGAGGGGCGGAAT+GGG | 0.329826 | 5.1:-32621802 | MS.gene066574:CDS |
| ACCTTCATTGATATTATAGA+TGG | 0.346577 | 5.1:+32620557 | None:intergenic |
| CGTCCTGTCATGGTTGTTGT+GGG | 0.348484 | 5.1:-32609875 | MS.gene066574:CDS |
| TGAAAGTATACAATAGTATA+AGG | 0.348667 | 5.1:+32609570 | None:intergenic |
| ATGAATTTGTCCCTTGCCTC+TGG | 0.354711 | 5.1:+32621747 | None:intergenic |
| TCAGATATACATTGCCTTTG+AGG | 0.355219 | 5.1:-32620662 | MS.gene066574:intron |
| TGCTCCAGACGTCTTTCATC+AGG | 0.355280 | 5.1:+32610851 | None:intergenic |
| GATTGATGCCGAGCATATAT+TGG | 0.357659 | 5.1:-32618384 | MS.gene066574:CDS |
| GCCAAAGGATCAGGGAAGTA+TGG | 0.366202 | 5.1:+32618329 | None:intergenic |
| TTAAAGGGGCCAAACCTTGC+AGG | 0.373155 | 5.1:+32614348 | None:intergenic |
| TCCATACTTCCCTGATCCTT+TGG | 0.380479 | 5.1:-32618330 | MS.gene066574:CDS |
| TTGCATGCTGCAGCCGGACC+TGG | 0.386360 | 5.1:-32614861 | MS.gene066574:CDS |
| CCTCAAAAGAAGGTTCAGTT+TGG | 0.387035 | 5.1:-32623294 | MS.gene066574:CDS |
| GATTGGAACGCCAAAGGATC+AGG | 0.387624 | 5.1:+32618320 | None:intergenic |
| TGATCCTTTGGCGTTCCAAT+CGG | 0.388724 | 5.1:-32618318 | MS.gene066574:CDS |
| TCCTGTTAGTGATGTTGGCT+TGG | 0.389424 | 5.1:-32611782 | MS.gene066574:CDS |
| AGTTGACCAGCCAATTCTTC+AGG | 0.389659 | 5.1:-32620787 | MS.gene066574:intron |
| ATAACCGCGAATTTATTTCA+TGG | 0.390336 | 5.1:+32618249 | None:intergenic |
| ACTGGTTCTCTAGGAGGTTT+CGG | 0.390604 | 5.1:+32611811 | None:intergenic |
| CAAAGCTTCCTGCAGATGTT+GGG | 0.392306 | 5.1:-32611903 | MS.gene066574:CDS |
| TTGATACCGAGATATATAAA+AGG | 0.394184 | 5.1:-32612117 | MS.gene066574:intron |
| TCGGGAACTGGGAGTGGTGC+AGG | 0.398214 | 5.1:-32618299 | MS.gene066574:CDS |
| CAGACTTAGGTTGGATGAAA+AGG | 0.402207 | 5.1:-32608370 | MS.gene066574:CDS |
| TTTGCTCAGATGTCTTGATC+TGG | 0.402468 | 5.1:-32609843 | MS.gene066574:CDS |
| GAATTTATTTCATGGTCAAC+AGG | 0.404059 | 5.1:+32618257 | None:intergenic |
| ATCTGTGGAGAGGGGATCCT+TGG | 0.405492 | 5.1:-32618226 | MS.gene066574:CDS |
| CTGGTGTTTGTGTTTGTCAT+AGG | 0.408691 | 5.1:-32618705 | MS.gene066574:intron |
| GCAGATGTTGGGGATGAAAA+TGG | 0.408765 | 5.1:-32611892 | MS.gene066574:CDS |
| GTGCAGGTGGTTCGGTGTCT+AGG | 0.408892 | 5.1:-32618283 | MS.gene066574:CDS |
| GGACAGGGTGGATGTCGAAC+AGG | 0.413491 | 5.1:-32614402 | MS.gene066574:intron |
| GTGATGTTGGCTTGGTTCGC+AGG | 0.417771 | 5.1:-32611774 | MS.gene066574:intron |
| ATGGATTACCTGATGACAAC+GGG | 0.423181 | 5.1:-32618442 | MS.gene066574:CDS |
| TTGAGCTTCTAATTGAAAAT+GGG | 0.428672 | 5.1:-32613049 | MS.gene066574:CDS |
| TGATCTAATACCTGAAGAAT+TGG | 0.435554 | 5.1:+32620777 | None:intergenic |
| ACCAAGCCAACATCACTAAC+AGG | 0.435940 | 5.1:+32611781 | None:intergenic |
| TTTATATATCTCGGTATCAA+TGG | 0.437653 | 5.1:+32612120 | None:intergenic |
| AAGTCAATCTGAGGCAGCTC+AGG | 0.441780 | 5.1:+32622679 | None:intergenic |
| GTCAACTTGGTGATCAAGAA+TGG | 0.441997 | 5.1:+32620803 | None:intergenic |
| TTCTTCAGCAAGACCAGGTC+CGG | 0.443155 | 5.1:+32614848 | None:intergenic |
| ACAAAGTCCCCGTTGTCATC+AGG | 0.444127 | 5.1:+32618434 | None:intergenic |
| GATCTTCCTGTTAGTGATGT+TGG | 0.446214 | 5.1:-32611787 | MS.gene066574:CDS |
| TTCCAATCGGGAACTGGGAG+TGG | 0.446703 | 5.1:-32618305 | MS.gene066574:CDS |
| GCGTCCTGTCATGGTTGTTG+TGG | 0.449127 | 5.1:-32609876 | MS.gene066574:CDS |
| CTGTTCAAGCACAACGAAGC+TGG | 0.449286 | 5.1:-32610817 | MS.gene066574:CDS |
| GCCATCTATAATATCAATGA+AGG | 0.453373 | 5.1:-32620558 | MS.gene066574:intron |
| ATTACAGTGATTGTGGAACT+TGG | 0.455076 | 5.1:-32618898 | MS.gene066574:intron |
| TGAAACCATTAAAACGTTTG+AGG | 0.455321 | 5.1:+32622656 | None:intergenic |
| AAACCTTGCAGGAAGGTCAT+AGG | 0.455325 | 5.1:+32614359 | None:intergenic |
| TGGGAGTGGTGCAGGTGGTT+CGG | 0.455865 | 5.1:-32618291 | MS.gene066574:CDS |
| TGCTGAAGAATGTGCAACTT+TGG | 0.456288 | 5.1:-32614835 | MS.gene066574:intron |
| CCGGGGTTGCATGCTGCAGC+CGG | 0.456318 | 5.1:-32614867 | MS.gene066574:CDS |
| ACAGGACTAGAGTCTGTTCC+TGG | 0.456712 | 5.1:-32622703 | MS.gene066574:CDS |
| CTCCTCCTCATGTTTATCCC+GGG | 0.461639 | 5.1:+32611936 | None:intergenic |
| CCTCCTCCTCATGTTTATCC+CGG | 0.461976 | 5.1:+32611935 | None:intergenic |
| GATGAAGCACACAGCAGCCC+GGG | 0.464045 | 5.1:-32614885 | MS.gene066574:CDS |
| CTACATGTGCAGCTGGTTCA+CGG | 0.464684 | 5.1:+32612874 | None:intergenic |
| ATTACAGTGATTGTGGAACT+TGG | 0.467495 | 5.1:-32618569 | MS.gene066574:intron |
| GATCCTTTGGCGTTCCAATC+GGG | 0.469064 | 5.1:-32618317 | MS.gene066574:CDS |
| ATGAAAGACGTCTGGAGCAG+TGG | 0.470136 | 5.1:-32610847 | MS.gene066574:CDS |
| GTTACATTCGGTGTTGTGTC+TGG | 0.472426 | 5.1:-32618724 | MS.gene066574:CDS |
| ACAAAGCTTCCTGCAGATGT+TGG | 0.477079 | 5.1:-32611904 | MS.gene066574:CDS |
| GTAAGAATGATGAGAAGATA+AGG | 0.480601 | 5.1:+32623239 | None:intergenic |
| GCTGCTGTGTGCTTCATCCA+AGG | 0.484294 | 5.1:+32614890 | None:intergenic |
| TACATGTGCAGCTGGTTCAC+GGG | 0.484754 | 5.1:+32612875 | None:intergenic |
| CATACTTTGGACCAACTGTA+TGG | 0.486282 | 5.1:+32613129 | None:intergenic |
| CCGGCTGCAGCATGCAACCC+CGG | 0.487599 | 5.1:+32614867 | None:intergenic |
| AGATTCAAGCTTGCAATCAA+AGG | 0.487986 | 5.1:+32620582 | None:intergenic |
| TGCAGGAAGGTCATAGGCAT+TGG | 0.488102 | 5.1:+32614365 | None:intergenic |
| CGCGGTTATATCTGTGGAGA+GGG | 0.489553 | 5.1:-32618235 | MS.gene066574:CDS |
| CGAGGGGCGGAATGGGGAGT+TGG | 0.490686 | 5.1:-32621795 | MS.gene066574:CDS |
| GCTCAAGCAGTTTCTTCACC+AGG | 0.491458 | 5.1:+32622488 | None:intergenic |
| TGGTACTTCACAGCATACTT+TGG | 0.492620 | 5.1:+32613116 | None:intergenic |
| TAGAAAGATATCTAGAGTGC+AGG | 0.492958 | 5.1:+32610726 | None:intergenic |
| AGACCCTGATGAAAGACGTC+TGG | 0.498552 | 5.1:-32610855 | MS.gene066574:CDS |
| ACTGCTTACGAGGGGCGGAA+TGG | 0.499290 | 5.1:-32621803 | MS.gene066574:CDS |
| GGAAGATCCACTGGTTCTCT+AGG | 0.500592 | 5.1:+32611802 | None:intergenic |
| ATGCATAGGCAACCAGACTT+AGG | 0.500794 | 5.1:-32608383 | MS.gene066574:intron |
| GGATGAAGCACACAGCAGCC+CGG | 0.504734 | 5.1:-32614886 | MS.gene066574:CDS |
| TCCTGTCATGGTTGTTGTGG+GGG | 0.508530 | 5.1:-32609873 | MS.gene066574:CDS |
| TTGCTTGGATTTGGTGACCT+AGG | 0.510192 | 5.1:-32610782 | MS.gene066574:CDS |
| CATAGCAATGTTGAAGGAGA+AGG | 0.511435 | 5.1:-32621727 | MS.gene066574:intron |
| TTAGAAGTGAATGGAGGAAA+GGG | 0.512744 | 5.1:-32607020 | MS.gene066574:CDS |
| ATTCTAGGTTTGGAAGAAAG+TGG | 0.513838 | 5.1:-32607811 | MS.gene066574:intron |
| CGGCTGCAGCATGCAACCCC+GGG | 0.517512 | 5.1:+32614868 | None:intergenic |
| CATGGAGGGGTGGACAGTGA+GGG | 0.519000 | 5.1:-32609899 | MS.gene066574:CDS |
| GCACATGCTGGATTCATGTC+AGG | 0.519014 | 5.1:-32622730 | MS.gene066574:CDS |
| TGGCGTTCCAATCGGGAACT+GGG | 0.519175 | 5.1:-32618310 | MS.gene066574:CDS |
| CTTATAGCTACATGTGCAGC+TGG | 0.522334 | 5.1:+32612867 | None:intergenic |
| CACCACTCCCAGTTCCCGAT+TGG | 0.523193 | 5.1:+32618303 | None:intergenic |
| ATCTGAGGCAGCTCAGGACC+AGG | 0.523597 | 5.1:+32622685 | None:intergenic |
| CAAATTCATAGCAATGTTGA+AGG | 0.527626 | 5.1:-32621733 | MS.gene066574:CDS |
| GAAGAATTGGCTGGTCAACT+TGG | 0.528509 | 5.1:+32620790 | None:intergenic |
| ACTCAGCAATCCTCAAAAGA+AGG | 0.529490 | 5.1:-32623304 | MS.gene066574:CDS |
| AGGCATGACTTAGAAGTGAA+TGG | 0.531983 | 5.1:-32607029 | MS.gene066574:CDS |
| ATTCTTCTCTGGTCAACTCC+AGG | 0.534134 | 5.1:-32623174 | MS.gene066574:intron |
| TTGTTCCATTACAGTGATTG+TGG | 0.535253 | 5.1:-32618905 | MS.gene066574:intron |
| CTCAAGCAGTTTCTTCACCA+GGG | 0.535692 | 5.1:+32622489 | None:intergenic |
| AAAAGGTTACAACAAATACT+TGG | 0.536580 | 5.1:-32608353 | MS.gene066574:CDS |
| TTGTTACATTACAGTGATTG+TGG | 0.537916 | 5.1:-32618576 | MS.gene066574:intron |
| GTGGAAATCTGAATGAAAGA+TGG | 0.538239 | 5.1:+32623339 | None:intergenic |
| CAGTGATTGTGGAACTTGGA+AGG | 0.539095 | 5.1:-32618894 | MS.gene066574:CDS |
| GTGGCTGACGAAGCAGCCAC+AGG | 0.539335 | 5.1:+32618473 | None:intergenic |
| TAATTGAAAATGGGCTTCGA+AGG | 0.542031 | 5.1:-32613040 | MS.gene066574:intron |
| TTGACCATGAAATAAATTCG+CGG | 0.545534 | 5.1:-32618253 | MS.gene066574:CDS |
| GTCATTCTCCAATATATGCT+CGG | 0.545830 | 5.1:+32618376 | None:intergenic |
| CTTAGAAGTGAATGGAGGAA+AGG | 0.546609 | 5.1:-32607021 | MS.gene066574:CDS |
| CTAATACCTGAAGAATTGGC+TGG | 0.546682 | 5.1:+32620781 | None:intergenic |
| GTCCTGTCATGGTTGTTGTG+GGG | 0.548197 | 5.1:-32609874 | MS.gene066574:CDS |
| TTAGTCCTAAAGTTGGGTGA+AGG | 0.549515 | 5.1:+32608211 | None:intergenic |
| GGGAATGACTCCAGAGGCAA+GGG | 0.550474 | 5.1:-32621757 | MS.gene066574:CDS |
| GGATTCATGTCAGGAATCAC+AGG | 0.551319 | 5.1:-32622721 | MS.gene066574:CDS |
| CAGTGAGGGGCGTCCTGTCA+TGG | 0.555153 | 5.1:-32609885 | MS.gene066574:CDS |
| TCAACTTGGTGATCAAGAAT+GGG | 0.558649 | 5.1:+32620804 | None:intergenic |
| TGCTTACATGCAGATTATGC+TGG | 0.559098 | 5.1:+32608321 | None:intergenic |
| TCAAGCAGTTTCTTCACCAG+GGG | 0.567433 | 5.1:+32622490 | None:intergenic |
| TGCTTACGAGGGGCGGAATG+GGG | 0.568619 | 5.1:-32621801 | MS.gene066574:CDS |
| GTCTTTAATCAAAGACATGA+AGG | 0.568809 | 5.1:+32610874 | None:intergenic |
| TGGAGGAAAGGGTCTTATTG+TGG | 0.570246 | 5.1:-32607009 | MS.gene066574:CDS |
| TTAATCAAAGACATGAAGGC+AGG | 0.570540 | 5.1:+32610878 | None:intergenic |
| AGGATCAGGGAAGTATGGAC+TGG | 0.571908 | 5.1:+32618334 | None:intergenic |
| TCTTCAGCGGCAGACAATGA+AGG | 0.572725 | 5.1:+32610755 | None:intergenic |
| TCATGGAGGGGTGGACAGTG+AGG | 0.573225 | 5.1:-32609900 | MS.gene066574:CDS |
| GGATAAACATGAGGAGGAGG+TGG | 0.573551 | 5.1:-32611932 | MS.gene066574:CDS |
| TATCTCGGTATCAATGGTGT+TGG | 0.575060 | 5.1:+32612126 | None:intergenic |
| AATGAAAGATGGTAATGAAG+AGG | 0.576236 | 5.1:+32623350 | None:intergenic |
| AGGGAATGACTCCAGAGGCA+AGG | 0.577514 | 5.1:-32621758 | MS.gene066574:CDS |
| GCACATTCTTCAGCAAGACC+AGG | 0.578730 | 5.1:+32614843 | None:intergenic |
| GTGATTGTGGAACTTGGAAG+AGG | 0.581455 | 5.1:-32618563 | MS.gene066574:intron |
| TTATCCCGGGGAAAATAAAG+TGG | 0.581679 | 5.1:+32611949 | None:intergenic |
| TGAAAGACGTCTGGAGCAGT+GGG | 0.582514 | 5.1:-32610846 | MS.gene066574:CDS |
| TTACATTAGATCAAGTTCCA+AGG | 0.582948 | 5.1:-32618409 | MS.gene066574:CDS |
| GAGAACAAGTACTGCTTACG+AGG | 0.584541 | 5.1:-32621813 | MS.gene066574:CDS |
| GCTCCAGACGTCTTTCATCA+GGG | 0.584744 | 5.1:+32610852 | None:intergenic |
| TGCTCGGCATCAATCCACCT+TGG | 0.584925 | 5.1:+32618392 | None:intergenic |
| CCGGGATAAACATGAGGAGG+AGG | 0.587303 | 5.1:-32611935 | MS.gene066574:CDS |
| TCACTAACAGGAAGATCCAC+TGG | 0.587528 | 5.1:+32611793 | None:intergenic |
| CATATATTGGAGAATGACAA+TGG | 0.587766 | 5.1:-32618371 | MS.gene066574:CDS |
| TCCCCGGGATAAACATGAGG+AGG | 0.589908 | 5.1:-32611938 | MS.gene066574:CDS |
| ATGGAGGGGTGGACAGTGAG+GGG | 0.590872 | 5.1:-32609898 | MS.gene066574:CDS |
| AGATCCACTGGTTCTCTAGG+AGG | 0.591085 | 5.1:+32611805 | None:intergenic |
| GTTGGTGATTGTTCAGCAGA+GGG | 0.594398 | 5.1:-32621777 | MS.gene066574:CDS |
| TAAATTCGCGGTTATATCTG+TGG | 0.596309 | 5.1:-32618241 | MS.gene066574:CDS |
| CGTGCATACATAGTTACATT+CGG | 0.598134 | 5.1:-32618736 | MS.gene066574:CDS |
| AGAAAGATATCTAGAGTGCA+GGG | 0.600496 | 5.1:+32610727 | None:intergenic |
| ATGCCTATGACCTTCCTGCA+AGG | 0.600890 | 5.1:-32614362 | MS.gene066574:intron |
| TCTTGGATAGCATTCCTCAA+AGG | 0.601149 | 5.1:+32620648 | None:intergenic |
| GCATCCACCTCAAGGTTCCA+AGG | 0.601317 | 5.1:+32618209 | None:intergenic |
| CAGACAATGAAGGTCCGCCT+AGG | 0.601609 | 5.1:+32610765 | None:intergenic |
| ATTGGAACGCCAAAGGATCA+GGG | 0.603696 | 5.1:+32618321 | None:intergenic |
| AGGAAAGTTATCCATACAGT+TGG | 0.606138 | 5.1:-32613140 | MS.gene066574:CDS |
| AGTTGGTGATTGTTCAGCAG+AGG | 0.606424 | 5.1:-32621778 | MS.gene066574:CDS |
| GGGTGGATGTCGAACAGGGA+TGG | 0.607011 | 5.1:-32614397 | MS.gene066574:CDS |
| TCGTCAGCCACATCTCAAGA+TGG | 0.609805 | 5.1:-32618461 | MS.gene066574:CDS |
| GGAACTGGGAGTGGTGCAGG+TGG | 0.609947 | 5.1:-32618296 | MS.gene066574:CDS |
| CATTAGATCAAGTTCCAAGG+TGG | 0.610843 | 5.1:-32618406 | MS.gene066574:CDS |
| CAAGTACTGCTTACGAGGGG+CGG | 0.612792 | 5.1:-32621808 | MS.gene066574:CDS |
| CTCGGAAATGTACAGGCCTG+TGG | 0.615964 | 5.1:-32618489 | MS.gene066574:intron |
| ATAGGCAACCAGACTTAGGT+TGG | 0.617470 | 5.1:-32608379 | MS.gene066574:intron |
| AGGGGATCCTTGGAACCTTG+AGG | 0.618486 | 5.1:-32618216 | MS.gene066574:CDS |
| AAAGCTTCCTGCAGATGTTG+GGG | 0.620045 | 5.1:-32611902 | MS.gene066574:CDS |
| GACAGGGTGGATGTCGAACA+GGG | 0.620338 | 5.1:-32614401 | MS.gene066574:intron |
| TCACCACGGCATCCACCTCA+AGG | 0.620482 | 5.1:+32618201 | None:intergenic |
| TCCTCCTCATGTTTATCCCG+GGG | 0.622744 | 5.1:+32611937 | None:intergenic |
| GGTTACAACAAATACTTGGA+CGG | 0.626704 | 5.1:-32608349 | MS.gene066574:CDS |
| CTTGGATTTGGTGACCTAGG+CGG | 0.628988 | 5.1:-32610779 | MS.gene066574:CDS |
| TTTATTCAGGAACTGTGCGG+CGG | 0.630616 | 5.1:-32612198 | MS.gene066574:intron |
| ATTGCAATTGAAGCAGCCAA+AGG | 0.635712 | 5.1:+32623195 | None:intergenic |
| AGGTAATCCATCTTGAGATG+TGG | 0.637564 | 5.1:+32618454 | None:intergenic |
| AGGGGCCAAACCTTGCAGGA+AGG | 0.639018 | 5.1:+32614352 | None:intergenic |
| TCGCGGTTATATCTGTGGAG+AGG | 0.639885 | 5.1:-32618236 | MS.gene066574:CDS |
| GGATCCTTGGAACCTTGAGG+TGG | 0.640816 | 5.1:-32618213 | MS.gene066574:CDS |
| GTTCCCGATTGGAACGCCAA+AGG | 0.641045 | 5.1:+32618314 | None:intergenic |
| TTTCAGCTATCATGGAGGGG+TGG | 0.641446 | 5.1:-32609909 | MS.gene066574:intron |
| GATGGATTACCTGATGACAA+CGG | 0.641640 | 5.1:-32618443 | MS.gene066574:CDS |
| CTCGTTTGTTGAATTCACCA+CGG | 0.641898 | 5.1:+32618187 | None:intergenic |
| ACATGTGCAGCTGGTTCACG+GGG | 0.650199 | 5.1:+32612876 | None:intergenic |
| GGGAAGTATGGACTGGAAAG+CGG | 0.652905 | 5.1:+32618341 | None:intergenic |
| CGTGGTGAATTCAACAAACG+AGG | 0.654661 | 5.1:-32618186 | MS.gene066574:intron |
| GAACCTTGAGGTGGATGCCG+TGG | 0.655173 | 5.1:-32618204 | MS.gene066574:CDS |
| CGTTTGAGGAAGTCAATCTG+AGG | 0.656409 | 5.1:+32622670 | None:intergenic |
| ATGAAGCACACAGCAGCCCG+GGG | 0.658339 | 5.1:-32614884 | MS.gene066574:CDS |
| GAAACCTCCTAGAGAACCAG+TGG | 0.660058 | 5.1:-32611809 | MS.gene066574:CDS |
| GGGATGGTCAACTGCTCACG+CGG | 0.664051 | 5.1:+32607750 | None:intergenic |
| AGAACAAGTACTGCTTACGA+GGG | 0.671236 | 5.1:-32621812 | MS.gene066574:CDS |
| GTGCAGGGAATATTCTTCAG+CGG | 0.675378 | 5.1:+32610742 | None:intergenic |
| ACAGTTGCTTGTCGACAATG+TGG | 0.676189 | 5.1:-32608169 | MS.gene066574:intron |
| AAGATTTAAAGAGACACCCC+TGG | 0.680049 | 5.1:-32622506 | MS.gene066574:CDS |
| CATGACTTAGAAGTGAATGG+AGG | 0.680744 | 5.1:-32607026 | MS.gene066574:CDS |
| AGCAGAGGGAATGACTCCAG+AGG | 0.703314 | 5.1:-32621763 | MS.gene066574:CDS |
| GCGGTTATATCTGTGGAGAG+GGG | 0.707385 | 5.1:-32618234 | MS.gene066574:CDS |
| AAGCATTCTCTGCAGCAGTG+TGG | 0.712836 | 5.1:+32613096 | None:intergenic |
| GAACAAGTACTGCTTACGAG+GGG | 0.743478 | 5.1:-32621811 | MS.gene066574:CDS |
| TGGATTACCTGATGACAACG+GGG | 0.769830 | 5.1:-32618441 | MS.gene066574:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 32606974 | 32623379 | 32606974 | ID=MS.gene066574 |
| chr5.1 | mRNA | 32606974 | 32623379 | 32606974 | ID=MS.gene066574.t1;Parent=MS.gene066574 |
| chr5.1 | exon | 32623175 | 32623379 | 32623175 | ID=MS.gene066574.t1.exon1;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32623175 | 32623379 | 32623175 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32622662 | 32622751 | 32622662 | ID=MS.gene066574.t1.exon2;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32622662 | 32622751 | 32622662 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32622466 | 32622562 | 32622466 | ID=MS.gene066574.t1.exon3;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32622466 | 32622562 | 32622466 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32621728 | 32621851 | 32621728 | ID=MS.gene066574.t1.exon4;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32621728 | 32621851 | 32621728 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32620788 | 32620885 | 32620788 | ID=MS.gene066574.t1.exon5;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32620788 | 32620885 | 32620788 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32620559 | 32620680 | 32620559 | ID=MS.gene066574.t1.exon6;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32620559 | 32620680 | 32620559 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32618860 | 32618913 | 32618860 | ID=MS.gene066574.t1.exon7;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32618860 | 32618913 | 32618860 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32618706 | 32618766 | 32618706 | ID=MS.gene066574.t1.exon8;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32618706 | 32618766 | 32618706 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32618564 | 32618584 | 32618564 | ID=MS.gene066574.t1.exon9;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32618564 | 32618584 | 32618564 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32618187 | 32618496 | 32618187 | ID=MS.gene066574.t1.exon10;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32618187 | 32618496 | 32618187 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32614836 | 32614913 | 32614836 | ID=MS.gene066574.t1.exon11;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32614836 | 32614913 | 32614836 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32614363 | 32614418 | 32614363 | ID=MS.gene066574.t1.exon12;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32614363 | 32614418 | 32614363 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32613041 | 32613160 | 32613041 | ID=MS.gene066574.t1.exon13;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32613041 | 32613160 | 32613041 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32612868 | 32612938 | 32612868 | ID=MS.gene066574.t1.exon14;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32612868 | 32612938 | 32612868 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32612118 | 32612211 | 32612118 | ID=MS.gene066574.t1.exon15;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32612118 | 32612211 | 32612118 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32611775 | 32611978 | 32611775 | ID=MS.gene066574.t1.exon16;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32611775 | 32611978 | 32611775 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32610680 | 32610931 | 32610680 | ID=MS.gene066574.t1.exon17;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32610680 | 32610931 | 32610680 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32609817 | 32609925 | 32609817 | ID=MS.gene066574.t1.exon18;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32609817 | 32609925 | 32609817 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32609549 | 32609616 | 32609549 | ID=MS.gene066574.t1.exon19;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32609549 | 32609616 | 32609549 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32608328 | 32608397 | 32608328 | ID=MS.gene066574.t1.exon20;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32608328 | 32608397 | 32608328 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32608170 | 32608244 | 32608170 | ID=MS.gene066574.t1.exon21;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32608170 | 32608244 | 32608170 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32607735 | 32607826 | 32607735 | ID=MS.gene066574.t1.exon22;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32607735 | 32607826 | 32607735 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| chr5.1 | exon | 32606974 | 32607049 | 32606974 | ID=MS.gene066574.t1.exon23;Parent=MS.gene066574.t1 |
| chr5.1 | CDS | 32606974 | 32607049 | 32606974 | ID=cds.MS.gene066574.t1;Parent=MS.gene066574.t1 |
| Gene Sequence |
| Protein sequence |
