Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067428.t1 | XP_003597930.2 | 99.4 | 175 | 1 | 0 | 1 | 175 | 1 | 175 | 3.40E-93 | 350.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067428.t1 | P42322 | 37.8 | 172 | 100 | 2 | 1 | 167 | 1 | 170 | 1.6e-27 | 124.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067428.t1 | B7FH75 | 99.4 | 175 | 1 | 0 | 1 | 175 | 1 | 175 | 2.5e-93 | 350.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05548 | MS.gene067428 | 0.822456 | 2.35E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene067428 | MS.gene82121 | PPI |
| MS.gene067428 | MS.gene37035 | PPI |
| MS.gene067428 | MS.gene83474 | PPI |
| MS.gene067428 | MS.gene020092 | PPI |
| MS.gene022826 | MS.gene067428 | PPI |
| MS.gene067428 | MS.gene028111 | PPI |
| MS.gene067428 | MS.gene72809 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067428.t1 | MTR_2g104130 | 99.429 | 175 | 1 | 0 | 1 | 175 | 1 | 175 | 5.87e-128 | 356 |
| MS.gene067428.t1 | MTR_4g130410 | 70.286 | 175 | 52 | 0 | 1 | 175 | 1 | 175 | 1.37e-91 | 264 |
| MS.gene067428.t1 | MTR_4g130410 | 71.429 | 161 | 46 | 0 | 1 | 161 | 1 | 161 | 5.36e-84 | 245 |
| MS.gene067428.t1 | MTR_1g076650 | 27.517 | 149 | 94 | 5 | 22 | 161 | 3 | 146 | 1.56e-11 | 59.7 |
| MS.gene067428.t1 | MTR_1g071150 | 27.517 | 149 | 95 | 5 | 21 | 161 | 2 | 145 | 2.35e-11 | 58.9 |
| MS.gene067428.t1 | MTR_1g041285 | 26.797 | 153 | 98 | 5 | 22 | 165 | 3 | 150 | 2.44e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067428.t1 | AT3G18430 | 78.857 | 175 | 37 | 0 | 1 | 175 | 1 | 175 | 1.01e-101 | 290 |
| MS.gene067428.t1 | AT3G18430 | 78.857 | 175 | 37 | 0 | 1 | 175 | 1 | 175 | 1.01e-101 | 290 |
| MS.gene067428.t1 | AT3G18430 | 78.857 | 175 | 37 | 0 | 1 | 175 | 1 | 175 | 1.01e-101 | 290 |
| MS.gene067428.t1 | AT3G22930 | 27.152 | 151 | 96 | 5 | 24 | 165 | 28 | 173 | 5.16e-12 | 61.6 |
Find 24 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACAGGTTCTTGGCCATTC+TGG | 0.182957 | 2.4:-1484580 | MS.gene067428:intron |
| TGTACTAAATGCAGATAAAA+AGG | 0.239607 | 2.4:+1485588 | None:intergenic |
| GACATTGGATGATTTCATTA+AGG | 0.296420 | 2.4:-1484776 | MS.gene067428:intron |
| TTAAATTTGTCACAGGTTCT+TGG | 0.342309 | 2.4:-1484589 | MS.gene067428:intron |
| TTTGATTTCTCAGGAAGTCT+TGG | 0.366861 | 2.4:-1484842 | MS.gene067428:intron |
| TTGATTTCTCAGGAAGTCTT+GGG | 0.378929 | 2.4:-1484841 | MS.gene067428:intron |
| AGGTTACTCAAGATGGTTGA+TGG | 0.393812 | 2.4:-1485634 | MS.gene067428:CDS |
| TGTTATGAATCCACTTTCTC+AGG | 0.398536 | 2.4:-1486003 | MS.gene067428:intron |
| GGTGTCTTTCAAAGACATAT+TGG | 0.407546 | 2.4:-1485253 | MS.gene067428:CDS |
| ATTTGATTCAGTTTCTCAAC+AGG | 0.411656 | 2.4:-1486117 | MS.gene067428:intron |
| GAAGTTCTAAAAGATTTGTC+TGG | 0.444669 | 2.4:-1485231 | MS.gene067428:CDS |
| ATCCTGCTTCTTTCAGAACC+TGG | 0.474604 | 2.4:+1484818 | None:intergenic |
| TTCTCAGGAAGTCTTGGGCC+AGG | 0.475351 | 2.4:-1484836 | MS.gene067428:intron |
| CAACTTGACAGAAATGCTAA+AGG | 0.478282 | 2.4:-1486065 | MS.gene067428:CDS |
| TTTGCAGAGGTTACTCAAGA+TGG | 0.478552 | 2.4:-1485641 | MS.gene067428:intron |
| TGATATGATACCTGAGAAAG+TGG | 0.501757 | 2.4:+1485993 | None:intergenic |
| TCTGCATTTAGTACAAAAGC+TGG | 0.505330 | 2.4:-1485580 | MS.gene067428:CDS |
| GCTAACTCAATATGATATTG+AGG | 0.520808 | 2.4:-1486255 | MS.gene067428:CDS |
| GGCCAGGTTCTGAAAGAAGC+AGG | 0.530644 | 2.4:-1484820 | MS.gene067428:CDS |
| GTATATGATTCAGATCGTAA+TGG | 0.556045 | 2.4:-1485279 | MS.gene067428:CDS |
| AAAAGACTGCTACTTGACAT+TGG | 0.563411 | 2.4:-1484791 | MS.gene067428:CDS |
| AGTGGATTCATAACAAACTC+AGG | 0.575670 | 2.4:+1486011 | None:intergenic |
| TGATTCAGATCGTAATGGAA+AGG | 0.613377 | 2.4:-1485274 | MS.gene067428:CDS |
| CATGTCTGATGATCAAAGAG+AGG | 0.654475 | 2.4:-1485202 | MS.gene067428:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATGATTATCTAAATTATTT+AGG | + | chr2.4:1485377-1485396 | None:intergenic | 10.0% |
| !! | TTTATATTTAAATTTGTCAC+AGG | - | chr2.4:1486225-1486244 | MS.gene067428:intron | 15.0% |
| !!! | ATTTTCTTATATATCTCTTT+TGG | - | chr2.4:1486075-1486094 | MS.gene067428:CDS | 15.0% |
| !!! | TTTTATGAAAATGGTATATT+TGG | + | chr2.4:1485010-1485029 | None:intergenic | 15.0% |
| !!! | TTTTATTGGCTTTTATTTAA+TGG | + | chr2.4:1485879-1485898 | None:intergenic | 15.0% |
| !! | AAGAAAAAAAAAGATAAGGA+AGG | + | chr2.4:1484633-1484652 | None:intergenic | 20.0% |
| !! | ACACATTTATTTAATTGAGT+CGG | + | chr2.4:1484942-1484961 | None:intergenic | 20.0% |
| !! | ACTCAATTAAATAAATGTGT+TGG | - | chr2.4:1484942-1484961 | MS.gene067428:intron | 20.0% |
| !! | ATCATAAATTAGAAGATTTC+AGG | - | chr2.4:1485391-1485410 | MS.gene067428:intron | 20.0% |
| !! | GAAATGAAATATTCGTATAT+AGG | + | chr2.4:1485857-1485876 | None:intergenic | 20.0% |
| !! | GAAGAAGAAAAAAAAAGATA+AGG | + | chr2.4:1484637-1484656 | None:intergenic | 20.0% |
| !! | TATTCTTTATTAGTAGTGTT+TGG | - | chr2.4:1485325-1485344 | MS.gene067428:intron | 20.0% |
| !!! | ATGTGTTTACTATTTTATAC+AGG | - | chr2.4:1485112-1485131 | MS.gene067428:intron | 20.0% |
| !!! | GTTCTTTCTAATTGTAATTT+TGG | - | chr2.4:1486180-1486199 | MS.gene067428:intron | 20.0% |
| !!! | TGTGGAAAAAAGTTTTTTAT+TGG | + | chr2.4:1485893-1485912 | None:intergenic | 20.0% |
| !!! | TTTGAATTTTAAAGACTTTG+TGG | - | chr2.4:1485210-1485229 | MS.gene067428:CDS | 20.0% |
| !!! | TTTTTTTGAAGAAACTCTTT+TGG | - | chr2.4:1486110-1486129 | MS.gene067428:intron | 20.0% |
| ! | ACATTTATTTAATTGAGTCG+GGG | + | chr2.4:1484940-1484959 | None:intergenic | 25.0% |
| ! | ACTATAAGTACCCTAATTAA+AGG | + | chr2.4:1485489-1485508 | None:intergenic | 25.0% |
| ! | AGTATATCAAACTCAACATA+GGG | + | chr2.4:1485764-1485783 | None:intergenic | 25.0% |
| ! | CACATTTATTTAATTGAGTC+GGG | + | chr2.4:1484941-1484960 | None:intergenic | 25.0% |
| ! | TAGTATATCAAACTCAACAT+AGG | + | chr2.4:1485765-1485784 | None:intergenic | 25.0% |
| ! | TCAAGTAAGTCCTTTAATTA+GGG | - | chr2.4:1485476-1485495 | MS.gene067428:intron | 25.0% |
| ! | TGTACTAAATGCAGATAAAA+AGG | + | chr2.4:1485236-1485255 | None:intergenic | 25.0% |
| ! | TTCAAGTAAGTCCTTTAATT+AGG | - | chr2.4:1485475-1485494 | MS.gene067428:intron | 25.0% |
| !! | AAGAATAAAATCTGAAGCAA+TGG | + | chr2.4:1485312-1485331 | None:intergenic | 25.0% |
| !! | TACAGAAGCATATAGTTATT+TGG | + | chr2.4:1485650-1485669 | None:intergenic | 25.0% |
| AACATTAAGCAATCAAAGCA+AGG | + | chr2.4:1485053-1485072 | None:intergenic | 30.0% | |
| AATCATTTATGTATGTCCTG+TGG | - | chr2.4:1484606-1484625 | MS.gene067428:intron | 30.0% | |
| AGCAAAGTAAGTTTCCATTA+AGG | - | chr2.4:1485712-1485731 | MS.gene067428:intron | 30.0% | |
| ATTTGATTCAGTTTCTCAAC+AGG | - | chr2.4:1484704-1484723 | MS.gene067428:intron | 30.0% | |
| CTTTATTAGTAGTGTTTGGA+TGG | - | chr2.4:1485329-1485348 | MS.gene067428:intron | 30.0% | |
| GACATTGGATGATTTCATTA+AGG | - | chr2.4:1486045-1486064 | MS.gene067428:CDS | 30.0% | |
| GCTAACTCAATATGATATTG+AGG | - | chr2.4:1484566-1484585 | MS.gene067428:CDS | 30.0% | |
| GTATATGATTCAGATCGTAA+TGG | - | chr2.4:1485542-1485561 | MS.gene067428:intron | 30.0% | |
| TAACAATTGATGCACCTTAA+TGG | + | chr2.4:1485729-1485748 | None:intergenic | 30.0% | |
| TTAAATTTGTCACAGGTTCT+TGG | - | chr2.4:1486232-1486251 | MS.gene067428:CDS | 30.0% | |
| ! | AAAACGTACGTTCAACTTTT+TGG | + | chr2.4:1485273-1485292 | None:intergenic | 30.0% |
| ! | AAATGGTATATTTGGAACAG+TGG | + | chr2.4:1485002-1485021 | None:intergenic | 30.0% |
| ! | TTACTGTTCTTTGATTTCTC+AGG | - | chr2.4:1485970-1485989 | MS.gene067428:intron | 30.0% |
| !! | GAAGTTCTAAAAGATTTGTC+TGG | - | chr2.4:1485590-1485609 | MS.gene067428:CDS | 30.0% |
| AGAAGATGTCCACTATAAGT+AGG | - | chr2.4:1485076-1485095 | MS.gene067428:intron | 35.0% | |
| CAACTTGACAGAAATGCTAA+AGG | - | chr2.4:1484756-1484775 | MS.gene067428:intron | 35.0% | |
| GAAGATGTCCACTATAAGTA+GGG | - | chr2.4:1485077-1485096 | MS.gene067428:intron | 35.0% | |
| GGTGTCTTTCAAAGACATAT+TGG | - | chr2.4:1485568-1485587 | MS.gene067428:CDS | 35.0% | |
| GTTTGTGTACTTATTTGCAG+AGG | - | chr2.4:1485167-1485186 | MS.gene067428:intron | 35.0% | |
| TCTGCATTTAGTACAAAAGC+TGG | - | chr2.4:1485241-1485260 | MS.gene067428:CDS | 35.0% | |
| TGATATGATACCTGAGAAAG+TGG | + | chr2.4:1484831-1484850 | None:intergenic | 35.0% | |
| TGTTATGAATCCACTTTCTC+AGG | - | chr2.4:1484818-1484837 | MS.gene067428:CDS | 35.0% | |
| TTGATTTCTCAGGAAGTCTT+GGG | - | chr2.4:1485980-1485999 | MS.gene067428:intron | 35.0% | |
| ! | AAAAGACTGCTACTTGACAT+TGG | - | chr2.4:1486030-1486049 | MS.gene067428:CDS | 35.0% |
| ! | AGTGGATTCATAACAAACTC+AGG | + | chr2.4:1484813-1484832 | None:intergenic | 35.0% |
| ! | TGATTCAGATCGTAATGGAA+AGG | - | chr2.4:1485547-1485566 | MS.gene067428:intron | 35.0% |
| ! | TTTGATTTCTCAGGAAGTCT+TGG | - | chr2.4:1485979-1485998 | MS.gene067428:intron | 35.0% |
| !! | CATCCATTTTTACTCCAGAA+TGG | + | chr2.4:1486258-1486277 | None:intergenic | 35.0% |
| AAAAGATAAGGAAGGACCAC+AGG | + | chr2.4:1484625-1484644 | None:intergenic | 40.0% | |
| AGGTTACTCAAGATGGTTGA+TGG | - | chr2.4:1485187-1485206 | MS.gene067428:intron | 40.0% | |
| CATGTCTGATGATCAAAGAG+AGG | - | chr2.4:1485619-1485638 | MS.gene067428:CDS | 40.0% | |
| CGAATACAGCACAACTGAAA+AGG | + | chr2.4:1485920-1485939 | None:intergenic | 40.0% | |
| TATCCTAGCCCTACTTATAG+TGG | + | chr2.4:1485088-1485107 | None:intergenic | 40.0% | |
| TTTGCAGAGGTTACTCAAGA+TGG | - | chr2.4:1485180-1485199 | MS.gene067428:intron | 40.0% | |
| !! | TGGCCATTCTGGAGTAAAAA+TGG | - | chr2.4:1486252-1486271 | MS.gene067428:CDS | 40.0% |
| ATCCTGCTTCTTTCAGAACC+TGG | + | chr2.4:1486006-1486025 | None:intergenic | 45.0% | |
| TGTCCACTATAAGTAGGGCT+AGG | - | chr2.4:1485082-1485101 | MS.gene067428:intron | 45.0% | |
| ! | CACAACTGAAAAGGTGTGTG+TGG | + | chr2.4:1485911-1485930 | None:intergenic | 45.0% |
| !!! | TTTATTTTATTTTATGAAAA+TGG | + | chr2.4:1485019-1485038 | None:intergenic | 5.0% |
| TCACAGGTTCTTGGCCATTC+TGG | - | chr2.4:1486241-1486260 | MS.gene067428:CDS | 50.0% | |
| TTCTCAGGAAGTCTTGGGCC+AGG | - | chr2.4:1485985-1486004 | MS.gene067428:intron | 55.0% | |
| !! | GGCCAGGTTCTGAAAGAAGC+AGG | - | chr2.4:1486001-1486020 | MS.gene067428:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 1484546 | 1486297 | 1484546 | ID=MS.gene067428 |
| chr2.4 | mRNA | 1484546 | 1486297 | 1484546 | ID=MS.gene067428.t1;Parent=MS.gene067428 |
| chr2.4 | exon | 1486228 | 1486297 | 1486228 | ID=MS.gene067428.t1.exon1;Parent=MS.gene067428.t1 |
| chr2.4 | CDS | 1486228 | 1486297 | 1486228 | ID=cds.MS.gene067428.t1;Parent=MS.gene067428.t1 |
| chr2.4 | exon | 1486004 | 1486128 | 1486004 | ID=MS.gene067428.t1.exon2;Parent=MS.gene067428.t1 |
| chr2.4 | CDS | 1486004 | 1486128 | 1486004 | ID=cds.MS.gene067428.t1;Parent=MS.gene067428.t1 |
| chr2.4 | exon | 1485560 | 1485656 | 1485560 | ID=MS.gene067428.t1.exon3;Parent=MS.gene067428.t1 |
| chr2.4 | CDS | 1485560 | 1485656 | 1485560 | ID=cds.MS.gene067428.t1;Parent=MS.gene067428.t1 |
| chr2.4 | exon | 1485203 | 1485312 | 1485203 | ID=MS.gene067428.t1.exon4;Parent=MS.gene067428.t1 |
| chr2.4 | CDS | 1485203 | 1485312 | 1485203 | ID=cds.MS.gene067428.t1;Parent=MS.gene067428.t1 |
| chr2.4 | exon | 1484777 | 1484851 | 1484777 | ID=MS.gene067428.t1.exon5;Parent=MS.gene067428.t1 |
| chr2.4 | CDS | 1484777 | 1484851 | 1484777 | ID=cds.MS.gene067428.t1;Parent=MS.gene067428.t1 |
| chr2.4 | exon | 1484546 | 1484596 | 1484546 | ID=MS.gene067428.t1.exon6;Parent=MS.gene067428.t1 |
| chr2.4 | CDS | 1484546 | 1484596 | 1484546 | ID=cds.MS.gene067428.t1;Parent=MS.gene067428.t1 |
| Gene Sequence |
| Protein sequence |
