AlfalfaGEDB | MS.gene067820

M. sativa cultivar XinJiangDaYe / MS.gene067820

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067820.t1	XP_003624637.1	100	33	0	0	38	70	206	238	3.30E-07	63.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067820.t1	Q2HVN6	100.0	33	0	0	38	70	206	238	2.4e-07	63.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04915	MS.gene067820	0.837567	4.82E-57	-1.69E-46
MS.gene04917	MS.gene067820	0.838412	2.92E-57	-1.69E-46
MS.gene04998	MS.gene067820	0.805711	1.17E-49	-1.69E-46
MS.gene050654	MS.gene067820	0.804687	1.92E-49	-1.69E-46
MS.gene051184	MS.gene067820	0.803547	3.31E-49	-1.69E-46
MS.gene051676	MS.gene067820	0.810122	1.35E-50	-1.69E-46
MS.gene052296	MS.gene067820	0.86052	1.95E-63	-1.69E-46
MS.gene052342	MS.gene067820	0.814772	1.30E-51	-1.69E-46
MS.gene052434	MS.gene067820	0.816857	4.45E-52	-1.69E-46
MS.gene056752	MS.gene067820	0.814816	1.27E-51	-1.69E-46
MS.gene058429	MS.gene067820	0.807737	4.37E-50	-1.69E-46
MS.gene06011	MS.gene067820	0.809958	1.46E-50	-1.69E-46
MS.gene060259	MS.gene067820	0.836234	1.05E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067820.t1	MTR_7g085850	100.000	47	0	0	24	70	192	238	2.56e-24	90.9
MS.gene067820.t1	MTR_6g028130	71.429	42	12	0	21	62	364	405	2.85e-12	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 14 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCTTCTGTTTCAGCTTACT+AGG	0.366926	7.1:+19325263	None:intergenic
TTTCAGCTTACTAGGATCAT+TGG	0.393959	7.1:+19325271	None:intergenic
TGCAAAGTTAGAAGCACTCT+TGG	0.412053	7.1:-19325228	MS.gene067820:CDS
CAGATTAAAGAAGGTGAAGA+TGG	0.424543	7.1:-19325182	MS.gene067820:CDS
AGAAGCACTCTTGGAAAAGA+AGG	0.514342	7.1:-19325219	MS.gene067820:CDS
AAAAGAAGGCTGATCTAGAA+AGG	0.535925	7.1:-19325205	MS.gene067820:CDS
CTAGAAAGGCAGATTAAAGA+AGG	0.541104	7.1:-19325191	MS.gene067820:CDS
ATCCTCAATCTTGATTTGAG+TGG	0.542150	7.1:+19325949	None:intergenic
AAGATTGAGGATAAATCTGC+AGG	0.542679	7.1:-19325938	MS.gene067820:CDS
AGGTGGTGTAAAAGAAGATA+AGG	0.558774	7.1:-19325918	MS.gene067820:intron
CTCCACTCAAATCAAGATTG+AGG	0.573595	7.1:-19325951	MS.gene067820:CDS
AGGTCAAGATATTAACGTGA+AGG	0.638283	7.1:-19325159	MS.gene067820:CDS
ATTAAAGAAGGTGAAGATGG+AGG	0.660144	7.1:-19325179	MS.gene067820:CDS
ATTGAGGATAAATCTGCAGG+TGG	0.690052	7.1:-19325935	MS.gene067820:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGTAAGAAAAAGAAGAATA+AGG	-	chr7.1:19325624-19325643	MS.gene067820:intron	20.0%
!	AAAGAAGATGATAAGAAAGA+AGG	-	chr7.1:19325751-19325770	MS.gene067820:intron	25.0%
!	ATAAGGATGAAGATAAAGAT+GGG	-	chr7.1:19325266-19325285	MS.gene067820:CDS	25.0%
!	GAAAAAGAAAAAGGAAAAGA+AGG	-	chr7.1:19325483-19325502	MS.gene067820:intron	25.0%
!	GAAAAAGAAGGATAAAAATG+AGG	-	chr7.1:19325657-19325676	MS.gene067820:intron	25.0%
	AAAAAGGAAAAGAAGGAGAA+GGG	-	chr7.1:19325490-19325509	MS.gene067820:intron	30.0%
	AAAGAAGGATAAAAATGAGG+AGG	-	chr7.1:19325660-19325679	MS.gene067820:intron	30.0%
	AGAGAAAGAGAAGAAACATA+AGG	-	chr7.1:19325249-19325268	MS.gene067820:CDS	30.0%
	CATAAGGATGAAGATAAAGA+TGG	-	chr7.1:19325265-19325284	MS.gene067820:CDS	30.0%
	GAAAAAGGAAAAGAAGGATA+AGG	-	chr7.1:19325291-19325310	MS.gene067820:intron	30.0%
	GAAGAAATCTAAGAAAGACA+AGG	-	chr7.1:19325543-19325562	MS.gene067820:intron	30.0%
	GAAGAAGAAAAAAGAAGACA+AGG	-	chr7.1:19325783-19325802	MS.gene067820:intron	30.0%
	GAAGAAGAAAAAGGAAAAGA+AGG	-	chr7.1:19325390-19325409	MS.gene067820:intron	30.0%
	GACAAGAATGAAGATGATAA+GGG	-	chr7.1:19325586-19325605	MS.gene067820:intron	30.0%
	GGATAAGAAAGAGAAAAAGA+AGG	-	chr7.1:19325645-19325664	MS.gene067820:intron	30.0%
	GGATAAGGAGAAAAAGAAAA+AGG	-	chr7.1:19325474-19325493	MS.gene067820:intron	30.0%
	TAAGGATGAAGATAAAGATG+GGG	-	chr7.1:19325267-19325286	MS.gene067820:CDS	30.0%
!!	ATCTTCTTTTTCACATTTCG+CGG	+	chr7.1:19325740-19325759	None:intergenic	30.0%
	AAAAGAAGGCTGATCTAGAA+AGG	-	chr7.1:19325905-19325924	MS.gene067820:intron	35.0%
	AAAGAAGATGGAGACAAAGA+TGG	-	chr7.1:19325514-19325533	MS.gene067820:intron	35.0%
	AAGATTGAGGATAAATCTGC+AGG	-	chr7.1:19325172-19325191	MS.gene067820:CDS	35.0%
	AGATAAAGATGGGGAGAAAA+AGG	-	chr7.1:19325276-19325295	MS.gene067820:CDS	35.0%
	AGGTCAAGATATTAACGTGA+AGG	-	chr7.1:19325951-19325970	MS.gene067820:CDS	35.0%
	AGGTGGTGTAAAAGAAGATA+AGG	-	chr7.1:19325192-19325211	MS.gene067820:CDS	35.0%
	ATTAAAGAAGGTGAAGATGG+AGG	-	chr7.1:19325931-19325950	MS.gene067820:CDS	35.0%
	CAGATTAAAGAAGGTGAAGA+TGG	-	chr7.1:19325928-19325947	MS.gene067820:CDS	35.0%
	CTAGAAAGGCAGATTAAAGA+AGG	-	chr7.1:19325919-19325938	MS.gene067820:CDS	35.0%
	GAAAAAGGAAAAGAAGGAGA+AGG	-	chr7.1:19325489-19325508	MS.gene067820:intron	35.0%
	GAAAGACAAGGAGAAGAAAA+AGG	-	chr7.1:19325555-19325574	MS.gene067820:intron	35.0%
	GAAGAAGAAGAAATCTGGAA+AGG	-	chr7.1:19325810-19325829	MS.gene067820:intron	35.0%
	GAAGAGAAGAAGAAGAAATC+TGG	-	chr7.1:19325805-19325824	MS.gene067820:intron	35.0%
	GGACAAGAATGAAGATGATA+AGG	-	chr7.1:19325585-19325604	MS.gene067820:intron	35.0%
	GGAGAAGAAAAAGGAAAAGA+AGG	-	chr7.1:19325564-19325583	MS.gene067820:intron	35.0%
	GGTTGAGATAGAAAAAGATC+TGG	-	chr7.1:19325213-19325232	MS.gene067820:CDS	35.0%
	GTTGATGTAGAGAATGTCAA+GGG	-	chr7.1:19325427-19325446	MS.gene067820:intron	35.0%
	TTTCAGCTTACTAGGATCAT+TGG	+	chr7.1:19325842-19325861	None:intergenic	35.0%
!	AGAAGATGGTGATGAAAAGA+AGG	-	chr7.1:19325453-19325472	MS.gene067820:intron	35.0%
!	AGTTGATGTAGAGAATGTCA+AGG	-	chr7.1:19325426-19325445	MS.gene067820:intron	35.0%
!	ATCCTCAATCTTGATTTGAG+TGG	+	chr7.1:19325164-19325183	None:intergenic	35.0%
!	TGGTGATGAAAAGAAGGATA+AGG	-	chr7.1:19325459-19325478	MS.gene067820:intron	35.0%
	AAATGAGGGCAAGGATAAGA+AGG	-	chr7.1:19325360-19325379	MS.gene067820:intron	40.0%
	AAGGAGAAGGGAAAAGAAGA+TGG	-	chr7.1:19325502-19325521	MS.gene067820:intron	40.0%
	AATGTCAAGGGTGAAGAAGA+TGG	-	chr7.1:19325439-19325458	MS.gene067820:intron	40.0%
	AGCTTCTGTTTCAGCTTACT+AGG	+	chr7.1:19325850-19325869	None:intergenic	40.0%
	ATTGAGGATAAATCTGCAGG+TGG	-	chr7.1:19325175-19325194	MS.gene067820:CDS	40.0%
	CAAAACTGATGTAGAGGAGA+AGG	-	chr7.1:19325336-19325355	MS.gene067820:intron	40.0%
	CTCCACTCAAATCAAGATTG+AGG	-	chr7.1:19325159-19325178	MS.gene067820:CDS	40.0%
	GAAGGACAAAACTGATGTAG+AGG	-	chr7.1:19325330-19325349	MS.gene067820:intron	40.0%
	GAAGGATAAAAATGAGGAGG+AGG	-	chr7.1:19325663-19325682	MS.gene067820:intron	40.0%
	GAAGGATAAGGAGAAGAAGA+AGG	-	chr7.1:19325303-19325322	MS.gene067820:intron	40.0%
	GAATGAAGATGATAAGGGTG+AGG	-	chr7.1:19325591-19325610	MS.gene067820:intron	40.0%
	GGACAAGGAGAAGAAGAAAA+AGG	-	chr7.1:19325381-19325400	MS.gene067820:intron	40.0%
	GGAGAAGAAGAAGGAAAAGA+AGG	-	chr7.1:19325312-19325331	MS.gene067820:intron	40.0%
	GTAGAGGAGAAGGAAAATGA+GGG	-	chr7.1:19325346-19325365	MS.gene067820:intron	40.0%
	TGGGGAGAAAAAGGAAAAGA+AGG	-	chr7.1:19325285-19325304	MS.gene067820:intron	40.0%
	TGTAGAGGAGAAGGAAAATG+AGG	-	chr7.1:19325345-19325364	MS.gene067820:intron	40.0%
!	AAGAGGGCAAAGTATCTGTA+AGG	-	chr7.1:19325695-19325714	MS.gene067820:intron	40.0%
!	AGAGGGCAAAGTATCTGTAA+GGG	-	chr7.1:19325696-19325715	MS.gene067820:intron	40.0%
!!	AGAAGCACTCTTGGAAAAGA+AGG	-	chr7.1:19325891-19325910	MS.gene067820:intron	40.0%
!!	TGCAAAGTTAGAAGCACTCT+TGG	-	chr7.1:19325882-19325901	MS.gene067820:intron	40.0%
	GAGGAGGATGAGAAAGAAGA+GGG	-	chr7.1:19325679-19325698	MS.gene067820:intron	45.0%
	GATAAGGGTGAGGATGTTGA+TGG	-	chr7.1:19325601-19325620	MS.gene067820:intron	45.0%
	GGAGAAGGAAAATGAGGGCA+AGG	-	chr7.1:19325351-19325370	MS.gene067820:intron	50.0%
	GGAGGAGGATGAGAAAGAAG+AGG	-	chr7.1:19325678-19325697	MS.gene067820:intron	50.0%
	GGGCAAGGATAAGAAGGACA+AGG	-	chr7.1:19325366-19325385	MS.gene067820:intron	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	19325151	19325981	19325151	ID=MS.gene067820
chr7.1	mRNA	19325151	19325981	19325151	ID=MS.gene067820.t1;Parent=MS.gene067820
chr7.1	exon	19325919	19325981	19325919	ID=MS.gene067820.t1.exon1;Parent=MS.gene067820.t1
chr7.1	CDS	19325919	19325981	19325919	ID=cds.MS.gene067820.t1;Parent=MS.gene067820.t1
chr7.1	exon	19325151	19325300	19325151	ID=MS.gene067820.t1.exon2;Parent=MS.gene067820.t1
chr7.1	CDS	19325151	19325300	19325151	ID=cds.MS.gene067820.t1;Parent=MS.gene067820.t1

Gene Sequence

>MS.gene067820

ATGGAAGACTCCACTCAAATCAAGATTGAGGATAAATCTGCAGGTGGTGTAAAAGAAGATAAGGTTGAGATAGAAAAAGATCTGGAGATTAAATCAGTAGAGAAAGAGAAGAAACATAAGGATGAAGATAAAGATGGGGAGAAAAAGGAAAAGAAGGATAAGGAGAAGAAGAAGGAAAAGAAGGACAAAACTGATGTAGAGGAGAAGGAAAATGAGGGCAAGGATAAGAAGGACAAGGAGAAGAAGAAAAAGGAAAAGAAGGAAAAAGATGACAAAGTTGATGTAGAGAATGTCAAGGGTGAAGAAGATGGTGATGAAAAGAAGGATAAGGAGAAAAAGAAAAAGGAAAAGAAGGAGAAGGGAAAAGAAGATGGAGACAAAGATGGCGAAGAGAAGAAATCTAAGAAAGACAAGGAGAAGAAAAAGGAAAAGAAGGACAAGAATGAAGATGATAAGGGTGAGGATGTTGATGGAAGTAAGAAAAAGAAGAATAAGGATAAGAAAGAGAAAAAGAAGGATAAAAATGAGGAGGAGGATGAGAAAGAAGAGGGCAAAGTATCTGTAAGGGATATTGATATAGAAGAAACCGCGAAATGTGAAAAAGAAGATGATAAGAAAGAAGGTAAAGAGAAGAAGAAGAAAAAAGAAGACAAGGAAGAGAAGAAGAAGAAATCTGGAAAGGAGAAGACCAATGATCCTAGTAAGCTGAAACAGAAGCTTGAAAAAGTGAATGCAAAGTTAGAAGCACTCTTGGAAAAGAAGGCTGATCTAGAAAGGCAGATTAAAGAAGGTGAAGATGGAGGTCAAGATATTAACGTGAAGGACAAGTAA

Protein sequence

>MS.gene067820.t1

MEDSTQIKIEDKSAGGVKEDKEKTNDPSKLKQKLEKVNAKLEALLEKKADLERQIKEGEDGGQDINVKDK