Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067838.t1 | XP_003624662.1 | 90.5 | 168 | 15 | 1 | 25 | 191 | 35 | 202 | 8.60E-74 | 286.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067838.t1 | G7L0H2 | 90.5 | 168 | 15 | 1 | 25 | 191 | 35 | 202 | 6.2e-74 | 286.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene067838 | 0.845833 | 3.17E-59 | -1.69E-46 |
| MS.gene049350 | MS.gene067838 | 0.817022 | 4.09E-52 | -1.69E-46 |
| MS.gene049711 | MS.gene067838 | 0.811045 | 8.52E-51 | -1.69E-46 |
| MS.gene051719 | MS.gene067838 | 0.815966 | 7.05E-52 | -1.69E-46 |
| MS.gene051720 | MS.gene067838 | 0.809404 | 1.92E-50 | -1.69E-46 |
| MS.gene052463 | MS.gene067838 | 0.805156 | 1.53E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene067838 | 0.807096 | 5.97E-50 | -1.69E-46 |
| MS.gene05458 | MS.gene067838 | 0.806208 | 9.20E-50 | -1.69E-46 |
| MS.gene055144 | MS.gene067838 | 0.829434 | 5.16E-55 | -1.69E-46 |
| MS.gene055313 | MS.gene067838 | 0.803887 | 2.81E-49 | -1.69E-46 |
| MS.gene055465 | MS.gene067838 | 0.803752 | 3.00E-49 | -1.69E-46 |
| MS.gene055466 | MS.gene067838 | 0.802703 | 4.95E-49 | -1.69E-46 |
| MS.gene055469 | MS.gene067838 | 0.803113 | 4.07E-49 | -1.69E-46 |
| MS.gene056308 | MS.gene067838 | 0.803783 | 2.96E-49 | -1.69E-46 |
| MS.gene058339 | MS.gene067838 | 0.853469 | 2.34E-61 | -1.69E-46 |
| MS.gene058360 | MS.gene067838 | 0.817532 | 3.14E-52 | -1.69E-46 |
| MS.gene058852 | MS.gene067838 | 0.809227 | 2.10E-50 | -1.69E-46 |
| MS.gene059171 | MS.gene067838 | 0.802781 | 4.77E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067838.t1 | MTR_7g086130 | 89.286 | 168 | 17 | 1 | 25 | 191 | 35 | 202 | 1.53e-87 | 256 |
| MS.gene067838.t1 | MTR_7g086130 | 85.475 | 179 | 24 | 2 | 15 | 191 | 29 | 207 | 4.55e-87 | 255 |
| MS.gene067838.t1 | MTR_6g478190 | 43.750 | 64 | 36 | 0 | 24 | 87 | 37 | 100 | 1.92e-13 | 65.5 |
| MS.gene067838.t1 | MTR_5g087450 | 41.270 | 63 | 37 | 0 | 25 | 87 | 38 | 100 | 7.58e-12 | 62.8 |
| MS.gene067838.t1 | MTR_5g087470 | 37.634 | 93 | 49 | 3 | 25 | 108 | 37 | 129 | 8.79e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067838.t1 | AT1G54400 | 41.791 | 67 | 39 | 0 | 25 | 91 | 38 | 104 | 1.23e-11 | 60.8 |
Find 28 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATCTTCATTGCAATATTT+TGG | 0.150093 | 7.1:+19190394 | None:intergenic |
| TCTGGCATTTGTTCTTCTTC+TGG | 0.224256 | 7.1:+19190295 | None:intergenic |
| ACATATCAGAGTTTGGAATT+TGG | 0.240193 | 7.1:-19190238 | MS.gene067838:CDS |
| TACACAACAGACAACAAGTT+TGG | 0.336570 | 7.1:-19190169 | MS.gene067838:CDS |
| AGAGGTTGCTCTTAAATTCA+TGG | 0.354880 | 7.1:-19190122 | MS.gene067838:CDS |
| GTCCATTTGATGGAACTAAA+TGG | 0.359566 | 7.1:-19190444 | MS.gene067838:CDS |
| ATATCCTCATCTTCAAGTTC+TGG | 0.365913 | 7.1:+19190277 | None:intergenic |
| TTCCATTTAGTTCCATCAAA+TGG | 0.385038 | 7.1:+19190442 | None:intergenic |
| CGAATTCAAATCAACCATCT+TGG | 0.396315 | 7.1:-19190490 | MS.gene067838:CDS |
| AGTGGAGAGCGTCCATTTGA+TGG | 0.411520 | 7.1:-19190454 | MS.gene067838:CDS |
| GGTTGTGATAATTGTTGTAA+TGG | 0.450646 | 7.1:-19190101 | MS.gene067838:CDS |
| TCTTCTTCTGGTTCCTCTTG+AGG | 0.457813 | 7.1:+19190307 | None:intergenic |
| CCCTCGAGCTGGCTAAGACT+TGG | 0.471677 | 7.1:-19190982 | MS.gene067838:CDS |
| GGTTATTGTAAATTATCTTG+TGG | 0.496692 | 7.1:-19190080 | MS.gene067838:CDS |
| TGCAATGAAGATGCCATTCG+TGG | 0.501501 | 7.1:-19190385 | MS.gene067838:CDS |
| TATCAGAGTTTGGAATTTGG+AGG | 0.521412 | 7.1:-19190235 | MS.gene067838:CDS |
| TCCAAGTCTTAGCCAGCTCG+AGG | 0.535627 | 7.1:+19190981 | None:intergenic |
| TTACTATATACCCCTCGAGC+TGG | 0.546007 | 7.1:-19190993 | MS.gene067838:CDS |
| TTGCATGAAATTGCCACGAA+TGG | 0.562966 | 7.1:+19190372 | None:intergenic |
| AGCAAAACCGTCATGGCTCA+AGG | 0.573165 | 7.1:-19190052 | MS.gene067838:CDS |
| AATGCCAGAACTTGAAGATG+AGG | 0.591611 | 7.1:-19190281 | MS.gene067838:CDS |
| AGTTGATCTGATACCTCAAG+AGG | 0.599832 | 7.1:-19190320 | MS.gene067838:CDS |
| TGATTGACCTTGAGCCATGA+CGG | 0.602155 | 7.1:+19190045 | None:intergenic |
| CAAGTCTTAGCCAGCTCGAG+GGG | 0.609969 | 7.1:+19190983 | None:intergenic |
| CCAAGTCTTAGCCAGCTCGA+GGG | 0.618955 | 7.1:+19190982 | None:intergenic |
| GGATATGAGCAAAACCGTCA+TGG | 0.621622 | 7.1:-19190059 | MS.gene067838:CDS |
| GAGCTGGCTAAGACTTGGAA+AGG | 0.622032 | 7.1:-19190977 | MS.gene067838:intron |
| TGATGTAGAAACTACTAGAG+AGG | 0.643103 | 7.1:-19190140 | MS.gene067838:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATGTATCTAAATTATATA+TGG | - | chr7.1:19190357-19190376 | MS.gene067838:CDS | 10.0% |
| !!! | CATTGTTTTTGTTAAATTTA+CGG | + | chr7.1:19190460-19190479 | None:intergenic | 15.0% |
| !! | AAATGAAATTGAACTAAACA+AGG | + | chr7.1:19190384-19190403 | None:intergenic | 20.0% |
| !! | CAAAGATTTGTGAAATAAAA+TGG | - | chr7.1:19190224-19190243 | MS.gene067838:CDS | 20.0% |
| !! | CGATGATCAATAAATTTATT+AGG | + | chr7.1:19190195-19190214 | None:intergenic | 20.0% |
| !!! | CAATAAATTTATTAGGTTGA+AGG | + | chr7.1:19190188-19190207 | None:intergenic | 20.0% |
| !!! | CATTTTATTTCACAAATCTT+TGG | + | chr7.1:19190226-19190245 | None:intergenic | 20.0% |
| ! | GAAAAATACATATCAGAGTT+TGG | - | chr7.1:19190813-19190832 | MS.gene067838:intron | 25.0% |
| ! | GGTTATTGTAAATTATCTTG+TGG | - | chr7.1:19190978-19190997 | MS.gene067838:CDS | 25.0% |
| !!! | ATTTTGATCCTGTTTTTAAG+TGG | - | chr7.1:19190280-19190299 | MS.gene067838:CDS | 25.0% |
| ATACATAAAATTGACATGCC+TGG | + | chr7.1:19190345-19190364 | None:intergenic | 30.0% | |
| GATCAAAATCTTCATAAGAC+CGG | + | chr7.1:19190269-19190288 | None:intergenic | 30.0% | |
| GGAATTAAACCTACTAATGA+AGG | + | chr7.1:19190167-19190186 | None:intergenic | 30.0% | |
| GGTTGTGATAATTGTTGTAA+TGG | - | chr7.1:19190957-19190976 | MS.gene067838:intron | 30.0% | |
| TTATCACCAAAAAACCAAGA+TGG | + | chr7.1:19190585-19190604 | None:intergenic | 30.0% | |
| TTCAAATTTGCCTTAAGAGT+TGG | + | chr7.1:19190134-19190153 | None:intergenic | 30.0% | |
| ! | ACATATCAGAGTTTGGAATT+TGG | - | chr7.1:19190820-19190839 | MS.gene067838:intron | 30.0% |
| ! | CTTTATTTGAGAGTTATGAG+GGG | - | chr7.1:19190507-19190526 | MS.gene067838:CDS | 30.0% |
| ! | GCATCTTCATTGCAATATTT+TGG | + | chr7.1:19190667-19190686 | None:intergenic | 30.0% |
| ! | TTCCATTTAGTTCCATCAAA+TGG | + | chr7.1:19190619-19190638 | None:intergenic | 30.0% |
| !! | GCTTTATTTGAGAGTTATGA+GGG | - | chr7.1:19190506-19190525 | MS.gene067838:CDS | 30.0% |
| !!! | AATCAACCATCTTGGTTTTT+TGG | - | chr7.1:19190576-19190595 | MS.gene067838:intron | 30.0% |
| !!! | GGGGTTATTAATTTGTTTTC+AGG | - | chr7.1:19190526-19190545 | MS.gene067838:intron | 30.0% |
| !!! | GGTATCAGATCAACTTTTTT+TGG | + | chr7.1:19190733-19190752 | None:intergenic | 30.0% |
| !!! | TAGACTTTTTTCACAGACTA+GGG | - | chr7.1:19190484-19190503 | MS.gene067838:CDS | 30.0% |
| !!! | TTAGACTTTTTTCACAGACT+AGG | - | chr7.1:19190483-19190502 | MS.gene067838:CDS | 30.0% |
| AACACAAACATGCACATAAC+CGG | - | chr7.1:19190247-19190266 | MS.gene067838:CDS | 35.0% | |
| AAGACTTGGAAAGGTTAACA+AGG | - | chr7.1:19190090-19190109 | MS.gene067838:CDS | 35.0% | |
| AGAGGTTGCTCTTAAATTCA+TGG | - | chr7.1:19190936-19190955 | MS.gene067838:intron | 35.0% | |
| CGAATTCAAATCAACCATCT+TGG | - | chr7.1:19190568-19190587 | MS.gene067838:intron | 35.0% | |
| GTCCATTTGATGGAACTAAA+TGG | - | chr7.1:19190614-19190633 | MS.gene067838:intron | 35.0% | |
| TACACAACAGACAACAAGTT+TGG | - | chr7.1:19190889-19190908 | MS.gene067838:intron | 35.0% | |
| TAGCTCATAACCAACTCTTA+AGG | - | chr7.1:19190121-19190140 | MS.gene067838:CDS | 35.0% | |
| TGATGTAGAAACTACTAGAG+AGG | - | chr7.1:19190918-19190937 | MS.gene067838:intron | 35.0% | |
| ! | ATATCCTCATCTTCAAGTTC+TGG | + | chr7.1:19190784-19190803 | None:intergenic | 35.0% |
| ! | TATCAGAGTTTGGAATTTGG+AGG | - | chr7.1:19190823-19190842 | MS.gene067838:intron | 35.0% |
| !! | GGCTTTATTTGAGAGTTATG+AGG | - | chr7.1:19190505-19190524 | MS.gene067838:CDS | 35.0% |
| !!! | CTTGGTTTTTTGGTGATAAG+TGG | - | chr7.1:19190586-19190605 | MS.gene067838:intron | 35.0% |
| AATGCCAGAACTTGAAGATG+AGG | - | chr7.1:19190777-19190796 | MS.gene067838:intron | 40.0% | |
| AGTTGATCTGATACCTCAAG+AGG | - | chr7.1:19190738-19190757 | MS.gene067838:intron | 40.0% | |
| TCTCTACGCCACTTAAAAAC+AGG | + | chr7.1:19190291-19190310 | None:intergenic | 40.0% | |
| TCTGGCATTTGTTCTTCTTC+TGG | + | chr7.1:19190766-19190785 | None:intergenic | 40.0% | |
| TTGCATGAAATTGCCACGAA+TGG | + | chr7.1:19190689-19190708 | None:intergenic | 40.0% | |
| ! | AACCTACTAATGAAGGCACA+AGG | + | chr7.1:19190160-19190179 | None:intergenic | 40.0% |
| ! | ACCTACTAATGAAGGCACAA+GGG | + | chr7.1:19190159-19190178 | None:intergenic | 40.0% |
| GGATATGAGCAAAACCGTCA+TGG | - | chr7.1:19190999-19191018 | MS.gene067838:CDS | 45.0% | |
| TGATTGACCTTGAGCCATGA+CGG | + | chr7.1:19191016-19191035 | None:intergenic | 45.0% | |
| TGCAATGAAGATGCCATTCG+TGG | - | chr7.1:19190673-19190692 | MS.gene067838:intron | 45.0% | |
| TTACTATATACCCCTCGAGC+TGG | - | chr7.1:19190065-19190084 | MS.gene067838:CDS | 45.0% | |
| !! | TCTTCTTCTGGTTCCTCTTG+AGG | + | chr7.1:19190754-19190773 | None:intergenic | 45.0% |
| ACCGTTGAGCTTCATCTTCC+AGG | - | chr7.1:19190324-19190343 | MS.gene067838:CDS | 50.0% | |
| AGCAAAACCGTCATGGCTCA+AGG | - | chr7.1:19191006-19191025 | MS.gene067838:CDS | 50.0% | |
| GAGCTGGCTAAGACTTGGAA+AGG | - | chr7.1:19190081-19190100 | MS.gene067838:CDS | 50.0% | |
| GCCCTTGTGCCTTCATTAGT+AGG | - | chr7.1:19190155-19190174 | MS.gene067838:CDS | 50.0% | |
| GCCTGGAAGATGAAGCTCAA+CGG | + | chr7.1:19190328-19190347 | None:intergenic | 50.0% | |
| ! | GATGAAGCTCAACGGTGTCA+CGG | + | chr7.1:19190320-19190339 | None:intergenic | 50.0% |
| !! | AGTGGAGAGCGTCCATTTGA+TGG | - | chr7.1:19190604-19190623 | MS.gene067838:intron | 50.0% |
| CAAGTCTTAGCCAGCTCGAG+GGG | + | chr7.1:19190078-19190097 | None:intergenic | 55.0% | |
| CCAAGTCTTAGCCAGCTCGA+GGG | + | chr7.1:19190079-19190098 | None:intergenic | 55.0% | |
| TCCAAGTCTTAGCCAGCTCG+AGG | + | chr7.1:19190080-19190099 | None:intergenic | 55.0% | |
| CCCTCGAGCTGGCTAAGACT+TGG | - | chr7.1:19190076-19190095 | MS.gene067838:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 19190030 | 19191050 | 19190030 | ID=MS.gene067838 |
| chr7.1 | mRNA | 19190030 | 19191050 | 19190030 | ID=MS.gene067838.t1;Parent=MS.gene067838 |
| chr7.1 | exon | 19190978 | 19191050 | 19190978 | ID=MS.gene067838.t1.exon1;Parent=MS.gene067838.t1 |
| chr7.1 | CDS | 19190978 | 19191050 | 19190978 | ID=cds.MS.gene067838.t1;Parent=MS.gene067838.t1 |
| chr7.1 | exon | 19190030 | 19190532 | 19190030 | ID=MS.gene067838.t1.exon2;Parent=MS.gene067838.t1 |
| chr7.1 | CDS | 19190030 | 19190532 | 19190030 | ID=cds.MS.gene067838.t1;Parent=MS.gene067838.t1 |
| Gene Sequence |
| Protein sequence |
