AlfalfaGEDB | MS.gene068035

M. sativa cultivar XinJiangDaYe / MS.gene068035

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068035.t1	XP_003608424.1	97.1	35	1	0	1	35	1	35	3.00E-06	61.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068035.t1	G7JV73	97.1	35	1	0	1	35	1	35	2.2e-06	61.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050082	MS.gene068035	0.893059	8.94E-75	-1.69E-46
MS.gene051422	MS.gene068035	0.813985	1.94E-51	-1.69E-46
MS.gene053568	MS.gene068035	0.98617	6.67E-166	-1.69E-46
MS.gene053569	MS.gene068035	0.931429	3.83E-94	-1.69E-46
MS.gene057717	MS.gene068035	0.827538	1.48E-54	-1.69E-46
MS.gene058067	MS.gene068035	0.810171	1.32E-50	-1.69E-46
MS.gene058068	MS.gene068035	0.858046	1.08E-62	-1.69E-46
MS.gene061044	MS.gene068035	0.810222	1.28E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068035.t1	MTR_4g093940	93.750	128	3	1	1	128	1	123	1.89e-67	199
MS.gene068035.t1	MTR_4g093950	93.750	112	7	0	1	112	1	112	1.12e-49	155
MS.gene068035.t1	MTR_4g093910	92.857	112	8	0	1	112	1	112	1.10e-48	152
MS.gene068035.t1	MTR_4g093920	91.743	109	8	1	1	109	1	108	2.19e-47	149
MS.gene068035.t1	MTR_5g064530	65.132	152	27	4	1	128	1	150	8.34e-42	135
MS.gene068035.t1	MTR_5g064510	66.667	123	21	4	1	105	1	121	4.06e-36	121
MS.gene068035.t1	MTR_4g094678	69.620	79	6	3	17	92	1	64	1.75e-22	84.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 44 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGATTTCCTTGAGGCATATT+TGG	0.193132	4.3:+20784349	None:intergenic
GTGGGAATGGTAGGTAAATT+TGG	0.271860	4.3:+20784412	None:intergenic
GCAATAGATGGCGAGCTTCT+AGG	0.294792	4.3:+20784449	None:intergenic
GGTGGTAATGTAGTTGGTTT+TGG	0.298989	4.3:+20784385	None:intergenic
TAAAGCTGCAATGAAGGATT+TGG	0.355352	4.3:+20784501	None:intergenic
AGATGGAATTGTTGGAATAT+TGG	0.374933	4.3:+20784261	None:intergenic
GTGTTGTTGTTTGCAATAGA+TGG	0.376065	4.3:+20784437	None:intergenic
GGGAAAGTGGTTGGGATTGA+AGG	0.382492	4.3:+20784193	None:intergenic
GGCTAGAAGTTGAAGGTGGT+GGG	0.405223	4.3:+20784152	None:intergenic
TGAGGCATATTTGGAATTGA+AGG	0.405314	4.3:+20784358	None:intergenic
ATAGTGAGTTTAGGCATAGA+AGG	0.436994	4.3:+20784307	None:intergenic
GGGAAGGAGGGGAAAGTGGT+TGG	0.439412	4.3:+20784184	None:intergenic
GGAAGGAGGGGAAAGTGGTT+GGG	0.444157	4.3:+20784185	None:intergenic
GGCAAAGGTGGTAATGTAGT+TGG	0.444412	4.3:+20784379	None:intergenic
GGTTGGGATTGAAGGAAGGT+TGG	0.454159	4.3:+20784201	None:intergenic
AGTGGTGGCATAGTGAGTTT+AGG	0.457061	4.3:+20784298	None:intergenic
AAATGGGAAGGAGGGGAAAG+TGG	0.467197	4.3:+20784180	None:intergenic
GTTGGGATTGAAGGAAGGTT+GGG	0.469467	4.3:+20784202	None:intergenic
TTAGGCATAGAAGGGATAGT+AGG	0.471087	4.3:+20784316	None:intergenic
GGGCTAGAAGTTGAAGGTGG+TGG	0.472490	4.3:+20784151	None:intergenic
AAGAAGTAAAGCTGCAATGA+AGG	0.485067	4.3:+20784495	None:intergenic
GTTTAAGGGCTAGAAGTTGA+AGG	0.490262	4.3:+20784145	None:intergenic
GGAATATTGGTAGGAAGACT+TGG	0.503031	4.3:+20784274	None:intergenic
AAGTGGTTGGGATTGAAGGA+AGG	0.508086	4.3:+20784197	None:intergenic
TGCAATGAAGGATTTGGTTG+AGG	0.510590	4.3:+20784507	None:intergenic
TTTGGTAAAGTGGGAATGGT+AGG	0.519398	4.3:+20784403	None:intergenic
AGTGAAGTGGCTGCTGGCAA+TGG	0.520284	4.3:+20784226	None:intergenic
GGAATTGTTGGAATATTGGT+AGG	0.523994	4.3:+20784265	None:intergenic
ATGTTGAGAGATGGAATTGT+TGG	0.529497	4.3:+20784253	None:intergenic
TTGGTAGGAAGACTTGGAAG+TGG	0.530626	4.3:+20784280	None:intergenic
GAAGGGATAGTAGGCAATGG+AGG	0.560608	4.3:+20784325	None:intergenic
GAAGTGGCTGCTGGCAATGG+TGG	0.580562	4.3:+20784229	None:intergenic
TTTGGAATTGAAGGCAAAGG+TGG	0.580888	4.3:+20784367	None:intergenic
TTGGGAAGTGAAGTGGCTGC+TGG	0.584437	4.3:+20784220	None:intergenic
TCAATTCCAAATATGCCTCA+AGG	0.589431	4.3:-20784355	MS.gene068035:CDS
GCTAGAAGTTGAAGGTGGTG+GGG	0.609650	4.3:+20784153	None:intergenic
GTAGGAAGACTTGGAAGTGG+TGG	0.611131	4.3:+20784283	None:intergenic
TAAGGGCTAGAAGTTGAAGG+TGG	0.613687	4.3:+20784148	None:intergenic
ATATTTGGAATTGAAGGCAA+AGG	0.615183	4.3:+20784364	None:intergenic
TAGTGAGTTTAGGCATAGAA+GGG	0.616158	4.3:+20784308	None:intergenic
ATAGAAGGGATAGTAGGCAA+TGG	0.624968	4.3:+20784322	None:intergenic
AGGAAGGTTGGGAAGTGAAG+TGG	0.630070	4.3:+20784213	None:intergenic
AATGGTGGAATGTTGAGAGA+TGG	0.653205	4.3:+20784244	None:intergenic
AATGGAGGAAGATTTCCTTG+AGG	0.717776	4.3:+20784340	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGATGGAATTGTTGGAATAT+TGG	+	chr4.3:20784400-20784419	None:intergenic	30.0%
!	ATATTTGGAATTGAAGGCAA+AGG	+	chr4.3:20784297-20784316	None:intergenic	30.0%
	AAGAAGTAAAGCTGCAATGA+AGG	+	chr4.3:20784166-20784185	None:intergenic	35.0%
	AGATTTCCTTGAGGCATATT+TGG	+	chr4.3:20784312-20784331	None:intergenic	35.0%
	ATAGTGAGTTTAGGCATAGA+AGG	+	chr4.3:20784354-20784373	None:intergenic	35.0%
	ATGTTGAGAGATGGAATTGT+TGG	+	chr4.3:20784408-20784427	None:intergenic	35.0%
	GGAATTGTTGGAATATTGGT+AGG	+	chr4.3:20784396-20784415	None:intergenic	35.0%
	TAAAGCTGCAATGAAGGATT+TGG	+	chr4.3:20784160-20784179	None:intergenic	35.0%
	TAGTGAGTTTAGGCATAGAA+GGG	+	chr4.3:20784353-20784372	None:intergenic	35.0%
	TCAATTCCAAATATGCCTCA+AGG	-	chr4.3:20784303-20784322	MS.gene068035:CDS	35.0%
!	TGAGGCATATTTGGAATTGA+AGG	+	chr4.3:20784303-20784322	None:intergenic	35.0%
!!	GTGTTGTTGTTTGCAATAGA+TGG	+	chr4.3:20784224-20784243	None:intergenic	35.0%
!!!	GTAGTTGGTTTTGGTAAAGT+GGG	+	chr4.3:20784267-20784286	None:intergenic	35.0%
!!!	TGTAGTTGGTTTTGGTAAAG+TGG	+	chr4.3:20784268-20784287	None:intergenic	35.0%
	AATGGAGGAAGATTTCCTTG+AGG	+	chr4.3:20784321-20784340	None:intergenic	40.0%
	ATAGAAGGGATAGTAGGCAA+TGG	+	chr4.3:20784339-20784358	None:intergenic	40.0%
	GGAATATTGGTAGGAAGACT+TGG	+	chr4.3:20784387-20784406	None:intergenic	40.0%
	GTGGGAATGGTAGGTAAATT+TGG	+	chr4.3:20784249-20784268	None:intergenic	40.0%
	TTAGGCATAGAAGGGATAGT+AGG	+	chr4.3:20784345-20784364	None:intergenic	40.0%
	TTTGGTAAAGTGGGAATGGT+AGG	+	chr4.3:20784258-20784277	None:intergenic	40.0%
!	AATGGTGGAATGTTGAGAGA+TGG	+	chr4.3:20784417-20784436	None:intergenic	40.0%
!	TGCAATGAAGGATTTGGTTG+AGG	+	chr4.3:20784154-20784173	None:intergenic	40.0%
!	TTTGGAATTGAAGGCAAAGG+TGG	+	chr4.3:20784294-20784313	None:intergenic	40.0%
!!	AAGGTGGTGGGGAAAAAAAT+GGG	+	chr4.3:20784497-20784516	None:intergenic	40.0%
!!	GGTGGTAATGTAGTTGGTTT+TGG	+	chr4.3:20784276-20784295	None:intergenic	40.0%
!!!	TGGTTTTGGTAAAGTGGGAA+TGG	+	chr4.3:20784262-20784281	None:intergenic	40.0%
	AAGTGGTTGGGATTGAAGGA+AGG	+	chr4.3:20784464-20784483	None:intergenic	45.0%
	AGTGGTGGCATAGTGAGTTT+AGG	+	chr4.3:20784363-20784382	None:intergenic	45.0%
	GGGAAAAAAATGGGAAGGAG+GGG	+	chr4.3:20784488-20784507	None:intergenic	45.0%
	GGGGAAAAAAATGGGAAGGA+GGG	+	chr4.3:20784489-20784508	None:intergenic	45.0%
	GTTGGGATTGAAGGAAGGTT+GGG	+	chr4.3:20784459-20784478	None:intergenic	45.0%
	TAAGGGCTAGAAGTTGAAGG+TGG	+	chr4.3:20784513-20784532	None:intergenic	45.0%
	TGGGGAAAAAAATGGGAAGG+AGG	+	chr4.3:20784490-20784509	None:intergenic	45.0%
	TGGTGGGGAAAAAAATGGGA+AGG	+	chr4.3:20784493-20784512	None:intergenic	45.0%
	TTGGTAGGAAGACTTGGAAG+TGG	+	chr4.3:20784381-20784400	None:intergenic	45.0%
!	GGCAAAGGTGGTAATGTAGT+TGG	+	chr4.3:20784282-20784301	None:intergenic	45.0%
!!	GAAGGTGGTGGGGAAAAAAA+TGG	+	chr4.3:20784498-20784517	None:intergenic	45.0%
	AAATGGGAAGGAGGGGAAAG+TGG	+	chr4.3:20784481-20784500	None:intergenic	50.0%
	AGGAAGGTTGGGAAGTGAAG+TGG	+	chr4.3:20784448-20784467	None:intergenic	50.0%
	GAAGGGATAGTAGGCAATGG+AGG	+	chr4.3:20784336-20784355	None:intergenic	50.0%
	GCAATAGATGGCGAGCTTCT+AGG	+	chr4.3:20784212-20784231	None:intergenic	50.0%
	GCTAGAAGTTGAAGGTGGTG+GGG	+	chr4.3:20784508-20784527	None:intergenic	50.0%
	GGCTAGAAGTTGAAGGTGGT+GGG	+	chr4.3:20784509-20784528	None:intergenic	50.0%
	GGGAAAGTGGTTGGGATTGA+AGG	+	chr4.3:20784468-20784487	None:intergenic	50.0%
	GGTTGGGATTGAAGGAAGGT+TGG	+	chr4.3:20784460-20784479	None:intergenic	50.0%
	GTAGGAAGACTTGGAAGTGG+TGG	+	chr4.3:20784378-20784397	None:intergenic	50.0%
	AGTGAAGTGGCTGCTGGCAA+TGG	+	chr4.3:20784435-20784454	None:intergenic	55.0%
	GGAAGGAGGGGAAAGTGGTT+GGG	+	chr4.3:20784476-20784495	None:intergenic	55.0%
	GGGCTAGAAGTTGAAGGTGG+TGG	+	chr4.3:20784510-20784529	None:intergenic	55.0%
	TTGGGAAGTGAAGTGGCTGC+TGG	+	chr4.3:20784441-20784460	None:intergenic	55.0%
	GAAGTGGCTGCTGGCAATGG+TGG	+	chr4.3:20784432-20784451	None:intergenic	60.0%
	GGGAAGGAGGGGAAAGTGGT+TGG	+	chr4.3:20784477-20784496	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	20784147	20784533	20784147	ID=MS.gene068035
chr4.3	mRNA	20784147	20784533	20784147	ID=MS.gene068035.t1;Parent=MS.gene068035
chr4.3	exon	20784147	20784533	20784147	ID=MS.gene068035.t1.exon1;Parent=MS.gene068035.t1
chr4.3	CDS	20784147	20784533	20784147	ID=cds.MS.gene068035.t1;Parent=MS.gene068035.t1

Gene Sequence

>MS.gene068035

ATGGCCTCAACCAAATCCTTCATTGCAGCTTTACTTCTTGTTGTCACAATGTCAAGCATGAGCCTAGAAGCTCGCCATCTATTGCAAACAACAACACAACCAAATTTACCTACCATTCCCACTTTACCAAAACCAACTACATTACCACCTTTGCCTTCAATTCCAAATATGCCTCAAGGAAATCTTCCTCCATTGCCTACTATCCCTTCTATGCCTAAACTCACTATGCCACCACTTCCAAGTCTTCCTACCAATATTCCAACAATTCCATCTCTCAACATTCCACCATTGCCAGCAGCCACTTCACTTCCCAACCTTCCTTCAATCCCAACCACTTTCCCCTCCTTCCCATTTTTTTCCCCACCACCTTCAACTTCTAGCCCTTAA

Protein sequence

>MS.gene068035.t1

MASTKSFIAALLLVVTMSSMSLEARHLLQTTTQPNLPTIPTLPKPTTLPPLPSIPNMPQGNLPPLPTIPSMPKLTMPPLPSLPTNIPTIPSLNIPPLPAATSLPNLPSIPTTFPSFPFFSPPPSTSSP